CN102963985B - Biological preparation for balancing water quality for aquaculture - Google Patents
Biological preparation for balancing water quality for aquaculture Download PDFInfo
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- CN102963985B CN102963985B CN 201210502289 CN201210502289A CN102963985B CN 102963985 B CN102963985 B CN 102963985B CN 201210502289 CN201210502289 CN 201210502289 CN 201210502289 A CN201210502289 A CN 201210502289A CN 102963985 B CN102963985 B CN 102963985B
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Abstract
The invention discloses a biological preparation for balancing water quality for aquaculture. The biological preparation comprises the components of: sodium humate accounting for 49 percent, sodium thiosulfate accounting for 35 percent, biological flocculants accounting for 10 percent, bacillus subtilis accounting for 3 percent, streptococcus faecium accounting for 3 percent, wherein the bacillus subtilis is 3,000,000,000/g and the streptococcus faecium is 3,000,000,000/g. According to the biological preparation, the nutrition of a water body is balanced, the water quality is improved, the blue-green algae outburst is controlled, the growth of beneficial algae is activated, dissolved oxygen of the water body is increased, the content of beneficial microorganisms in the water body is increased, the occurrence of chronic nitrate and nitrous acid poison incidents of fishes can be prevented, the immune globulin and the antibody level of the fishes are improved, and the herd immunity of the fishes is improved; and by adding the streptococcus faecium, the true nutritive value of feed is improved, the concentration of ammoniacal nitrogen in excrements also can be reduced, and the pollution to the water quality is reduced.
Description
Technical field
The present invention relates to technical field of aquaculture, relate in particular to a kind of biotechnological formulation of used for aquiculture Balance Water Quality.
Background technology
Fast development along with culture fishery; particularly greatly develop today of mass-producing high-density breeding; the proportion of aquaculture in aquaculture continues to increase, and due to the continuous expansion of the market requirement, the cultivation density at aquaculture family is also continuing to increase; be accompanied by the lasting increasing of aquaculture density; the water quality deterioration situation of bringing can not be ignored, and is mainly manifested in: the water pollution that fish faeces causes, and human lives's sewage; the impact of rural sewage, cause water quality deterioration.Some raiser's water quality severe exacerbations, the cultivation such as the ammonia nitrogen in water body, nitrite, dissolved oxygen amount parameter continues to worsen; Useful algae activity level greatly reduces, and aging and situation stagnant water is day by day serious, and photosynthesis reduces, and shows that in one day, pH value does not change, or changes atomicly, is not suitable for the life growth of fish.Just because of the lasting deterioration of water quality environment, fish disease takes place frequently, and the raiser adopts medical treatment in a large number, particularly antibiotic abuse degree increases, thereby accelerates the deterioration of water quality situation, and the fish meat is had a negative impact, germ develops immunity to drugs, and the control of fish disease is brought to larger difficulty.
Aquaculture needed " fertile, alive, tender, refreshing ", need to be in Balance Water Quality could realize under the uniform condition of mushroom, algae.Mushroom is beneficial microorganism shared ratio in water quality, and form according to the certain bacterial strain of the needs of aquaculture, current highdensity cultivation causes water body Mesichthyes excrement discharge to strengthen, add in the situation that use in a large number feces of livestock and poultry as fish guano in freshwater aquiculture, in water body, ammonia nitrogen, nitrite content increase, and are necessary to keep in water body having a large amount of decomposition to absorb the bacterial strain of ight soil composition.
Algae is contained useful algae in aquaculture water, diatom and green alga are the bait of fish, whether the quantity of diatom and green alga and kind balance, can maintain sufficient photosynthesis, the breeding that increases dissolved oxygen amount and control harmful algae, it is large that the dissolved oxygen in water body is mainly that the photosynthesis of algae maintains accounting, dissolved oxygen accounting artificial and that atmosphere infiltrates water body by wind is few, maintain balance and the quality of algae, balanced useful algae need to keep pH value benign cycle in water body.But practical situation are the deteriorations along with water body environment, useful algae constantly reduces, harmful algae constantly increases, due to the ammonia nitrogen that can not control in water body, promoted the breeding of harmful algae, harmful algae consumes the oxygen in water in a large number, the fish harmful algae of can't ingesting, cause harmful algae quantity too much, the corpse after harmful algae death decomposes generation stench and objectionable impurities, continues to worsen water quality.
Cultivate good breeding environment, enhance productivity, reduce production costs and become the essential condition of aquaculture.And the existing kind of the biotechnological formulation for the aquaculture Balance Water Quality is seldom, too much drop at present antibiotics in the aquaculture process, the long-time use can be reduced water quality, affects the growth of aquaculture Mesichthyes.
Summary of the invention
Technical problem solved by the invention is to provide a kind of biotechnological formulation of used for aquiculture Balance Water Quality, and the balance water nutrition is controlled blue algae bloom, activate useful algal grown, increase the water body dissolved oxygen amount, improve beneficial microorganism content, to solve the shortcoming in the above-mentioned background technology.
Technical problem solved by the invention realizes by the following technical solutions:
A kind of biotechnological formulation of used for aquiculture Balance Water Quality, comprise Sodium salts humic acids (HA-Na), Sulfothiorine, biological flocculant, subtilis, streptococcus faecium; Wherein, Sodium salts humic acids accounts for 49%, and Sulfothiorine accounts for 35%, and biological flocculant accounts for 10%, and subtilis accounts for 3%, and streptococcus faecium accounts for 3%.
In the present invention, subtilis is preferably 30,000,000,000/gram.
In the present invention, streptococcus faecium is preferably 30,000,000,000/gram.
In the present invention, Sodium salts humic acids is a plant growth regulators, and its effect is similar to xanthohumic acid, therefore add in biotechnological formulation, to activate useful algal grown in water body, increases the water body dissolved oxygen amount.
In the present invention, Sulfothiorine is as the chronic nitrate of fish and the acidosic preventive of nitrous, current highdensity cultivation causes water body Mesichthyes excrement discharge to strengthen, add in the situation that use in a large number feces of livestock and poultry as fish guano in freshwater aquiculture, ammonia nitrogen in water body, nitrite content increases, to impel fish that poisoning probability occurs increases, in Nitrite and fish body, oxyphorase forms rapidly methemoglobin, in methemoglobin ferric ion in body free or cyano group that be combined with Terminal oxidase in conjunction with forming unsettled cyanmethemoglobin, and make enzyme avoid suppressing, cyanmethemoglobin can dissociate cryanide ion again at several minutes, therefore need supply rapidly the sulphur agent as Sulfothiorine, so that cryanide ion changes the low toxicity thiocyanate-into and excrete, and then removed the generation of fish poisoning.In the present invention, microbial flocculant is comprised of the macromolecule organic with flocculation activity of microorganisms, mainly contain glycoprotein, mucopolysaccharide, Mierocrystalline cellulose and nucleic acid etc., because microbial flocculant has overcome inorganic polymer and the intrinsic defect of Syn-Organic flocculants itself, the characteristic of high, the safe and harmless and readily biodegradable of its flocculation activity, finally realize non-pollution discharge, therefore add in biotechnological formulation, to improve water quality, the balance water nutrition.
In the present invention, produce subtilyne, polymyxin, nystatin, linear gramicidins isoreactivity material in subtilis thalli growth process, fish are had to obvious restraining effect the conditioned pathogen of pathogenic bacterium or autogenous infection; Subtilis can consume rapidly the free oxygen in environment, causes the enteron aisle hypoxemia, promotes the growth of useful anerobe, and produces the organic acid such as lactic acid, reduces the enteron aisle pH value, indirectly suppresses other pathogenic bacterium growth; Stimulate growing of fish immunity organ, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen cellular immunization and humoral immune function, improve the fish herd immunity; The multiple vitamin B group such as subtilis thalline energy synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising Fish internal interference element and scavenger cell.In the present invention, the lactic acid of streptococcus faecium secretion is L-type lactic acid, is called again nature lactic acid or physiology lactic acid, can by fish, be absorbed fully.Streptococcus faecium also can be resolved into acid amides and amino acid to partial protein, and the nitrogen-free extract of most carbohydrate is converted into to lactic acid, and makes the fiber deliquescing in feed, and the conversion specific absorption of feed is high.These special nutrition compositions that streptococcus faecium is decomposed have made up the auxotrophy of conventional bait, and the various cultured fishes young have been played to very important nutritional fortification and growth promoting function.In aquaculture system, added the digestibility that streptococcus faecium not only is conducive to improve feed, the also corresponding concentration that reduces the ammonia-state nitrogen in the movement, reduced excremental indigestion thing simultaneously, improved feeding environment, reduced pollution.
In the present invention, the antimicrobial substance that streptococcus faecium produces is peptide or protein mostly, is called as bacteriocin.Streptococcus faecium can secrete two kind bacteriocins, and a class only just has restraining effect to relevant bacterium, and antimicrobial spectrum is narrower, can hinder pathogenic micro-organism contact intestinal mucosa cells; Another kind of have a broad spectrum antibiotic activity, and they have good restraining effect to pathogenic bacterium as: Salmonellas, congratulate Salmonella and pseudomonas.So, add for a long time the generation that streptococcus faecium can reduce the diseases such as fish enteritis in feed.
Biotechnological formulation component based on above-mentioned a kind of used for aquiculture Balance Water Quality, its biotechnological formulation complete processing is: after the Sodium salts humic acids drying and crushing, hybrid packed with biological flocculant, adopt the dry sealing of Sulfothiorine; Subtilis and streptococcus faecium are hybrid packed, then by two kinds of hybrid packed biotechnological formulations that are encapsulated as.
beneficial effect
Balance water nutrition of the present invention, improve water quality, control blue algae bloom, activate useful algal grown, increase the water body dissolved oxygen amount, improve beneficial microorganism content in water body, the generation of the prevention chronic nitrate of fish and nitrous acid poisoning, and improve study on immunoglobulin of fishes and antibody horizontal, strengthen cellular immunization and humoral immune function, and then improve the fish herd immunity; The digestibility that not only is conducive to improve feed by adding streptococcus faecium, the while has also been reduced the concentration of the ammonia-state nitrogen in the movement, has reduced excremental indigestion thing, has improved feeding environment, has reduced the pollution to water quality.
Embodiment
Below give an actual example and describe the present invention.
In the present embodiment, a kind of biotechnological formulation of used for aquiculture Balance Water Quality comprises following component:
Sodium salts humic acids, Sulfothiorine, biological flocculant, subtilis, streptococcus faecium; Wherein, in biotechnological formulation, with 100 parts of weight, calculate, Sodium salts humic acids accounts for 49 parts, and Sulfothiorine accounts for 35 parts, and biological flocculant accounts for 10 parts, and subtilis accounts for 3 parts, and streptococcus faecium accounts for 3 parts.
In the present embodiment, subtilis is preferably 30,000,000,000/gram.
In the present embodiment, streptococcus faecium is preferably 30,000,000,000/gram.
In the present embodiment, Sodium salts humic acids is a plant growth regulators, and its effect is similar to xanthohumic acid, therefore add in biotechnological formulation, to activate useful algal grown in water body, increases the water body dissolved oxygen amount.
In the present embodiment, Sulfothiorine is as the chronic nitrate of fish and the acidosic preventive of nitrous, current highdensity cultivation causes water body Mesichthyes excrement discharge to strengthen, add in the situation that use in a large number feces of livestock and poultry as fish guano in freshwater aquiculture, ammonia nitrogen in water body, nitrite content increases, to impel fish that poisoning probability occurs increases, in Nitrite and fish body, oxyphorase forms rapidly methemoglobin, in methemoglobin ferric ion in body free or cyano group that be combined with Terminal oxidase in conjunction with forming unsettled cyanmethemoglobin, and make enzyme avoid suppressing, cyanmethemoglobin can dissociate cryanide ion again at several minutes, therefore need supply rapidly the sulphur agent as Sulfothiorine, so that cryanide ion changes the low toxicity thiocyanate-into and excrete, and then removed the generation of fish poisoning.In the present embodiment, microbial flocculant is comprised of the macromolecule organic with flocculation activity of microorganisms, mainly contain glycoprotein, mucopolysaccharide, Mierocrystalline cellulose and nucleic acid etc., because microbial flocculant has overcome inorganic polymer and the intrinsic defect of Syn-Organic flocculants itself, the characteristic of high, the safe and harmless and readily biodegradable of its flocculation activity, finally realize non-pollution discharge, therefore add in biotechnological formulation, to improve water quality, the balance water nutrition.
In the present embodiment, produce subtilyne, polymyxin, nystatin, linear gramicidins isoreactivity material in subtilis thalli growth process, fish are had to obvious restraining effect the conditioned pathogen of pathogenic bacterium or autogenous infection; Subtilis can consume rapidly the free oxygen in environment, causes the enteron aisle hypoxemia, promotes the growth of useful anerobe, and produces the organic acid such as lactic acid, reduces the enteron aisle pH value, indirectly suppresses other pathogenic bacterium growth; Stimulate growing of fish immunity organ, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen cellular immunization and humoral immune function, improve the fish herd immunity; The multiple vitamin B group such as subtilis thalline energy synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising Fish internal interference element and scavenger cell.In the present embodiment, the lactic acid of streptococcus faecium secretion is L-type lactic acid, is called again nature lactic acid or physiology lactic acid, can by fish, be absorbed fully.Streptococcus faecium also can be resolved into acid amides and amino acid to partial protein, and the nitrogen-free extract of most carbohydrate is converted into to lactic acid, and makes the fiber deliquescing in feed, and the conversion specific absorption of feed is high.These special nutrition compositions that streptococcus faecium is decomposed have made up the auxotrophy of conventional bait, and the various cultured fishes young have been played to very important nutritional fortification and growth promoting function.In aquaculture system, added the digestibility that streptococcus faecium not only is conducive to improve feed, the also corresponding concentration that reduces the ammonia-state nitrogen in the movement, reduced excremental indigestion thing simultaneously, improved feeding environment, reduced pollution.
In the present embodiment, the antimicrobial substance that streptococcus faecium produces is peptide or protein mostly, is called as bacteriocin.Streptococcus faecium can secrete two kind bacteriocins, and a class only just has restraining effect to relevant bacterium, and antimicrobial spectrum is narrower, can hinder pathogenic micro-organism contact intestinal mucosa cells; Another kind of have a broad spectrum antibiotic activity, and they have good restraining effect to pathogenic bacterium as: Salmonellas, congratulate Salmonella and pseudomonas.So, add for a long time the generation that streptococcus faecium can reduce the diseases such as fish enteritis in feed.
In the present embodiment, concrete using method is: first day use Sodium salts humic acids and biological flocculant are hybrid packed, consumption 1kg/ mu, midfeather one day, used subtilis and streptococcus faecium hybrid packed on the 3rd day, consumption 1kg/30 mu, in use, the changing conditions of close supervision water quality and fish, take corresponding measure in time, guarantees dissolved oxygen amount abundance in water body.
Above demonstration and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (4)
1. the biotechnological formulation of a used for aquiculture Balance Water Quality, is characterized in that, comprises Sodium salts humic acids (HA-Na), Sulfothiorine, biological flocculant, subtilis, streptococcus faecium; Wherein, Sodium salts humic acids accounts for 49%, and Sulfothiorine accounts for 35%, and biological flocculant accounts for 10%, and subtilis accounts for 3%, and streptococcus faecium accounts for 3%.
2. the biotechnological formulation of a kind of used for aquiculture Balance Water Quality according to claim 1, is characterized in that, subtilis is 30,000,000,000/gram.
3. the biotechnological formulation of a kind of used for aquiculture Balance Water Quality according to claim 1, is characterized in that, streptococcus faecium is 30,000,000,000/gram.
4. the biotechnological formulation of a kind of used for aquiculture Balance Water Quality according to claim 1, is characterized in that, complete processing is: after the Sodium salts humic acids drying and crushing, hybrid packed with biological flocculant, adopt the dry sealing of Sulfothiorine; Subtilis and streptococcus faecium are hybrid packed, then by two kinds of hybrid packed biotechnological formulations that are encapsulated as.
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Cited By (1)
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| CN103992187B (en) * | 2014-06-16 | 2016-04-20 | 蒋常德 | A kind of bio-bacterial manure for preventing and treating water body moss and preparation method thereof |
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| MY188872A (en) * | 2018-12-27 | 2022-01-11 | Univ Putra Malaysia | A composition for an immune?enhancing aquaculture pellet |
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Denomination of invention: Biological preparation for balancing water quality for aquaculture Effective date of registration: 20180315 Granted publication date: 20131225 Pledgee: China Merchants Bank Limited by Share Ltd Nanchang branch Pledgor: Jiangxi Province Hongji Bio-tech Co., Ltd. Registration number: 2018990000198 |