CN104278020A - Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent - Google Patents
Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent Download PDFInfo
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- CN104278020A CN104278020A CN201310572172.1A CN201310572172A CN104278020A CN 104278020 A CN104278020 A CN 104278020A CN 201310572172 A CN201310572172 A CN 201310572172A CN 104278020 A CN104278020 A CN 104278020A
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Abstract
The invention discloses a microbial water purifying agent for weever farming wastewater and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps: adding 40-60 parts by weight of magnetic silica beads with surface modification of C8 and C18 alkyl chains into 200-300 parts by weight of water; adding 200-300 parts by weight of a microbial reagent comprising 50-80 parts of oscillatoria, 40-80 parts of rhodomicrobium, 40-60 parts of nitrosococcus, 30-50 parts of nitrospina, 30-40 parts of crenothrix, 30-40 parts of pseudomonas aeruginosa, 3-6 parts of leucothrix, 3-6 parts of beggiatoa, 40-60 parts of zooflagellates, 40-60 parts of suctorians, 40-50 parts of dunaliella and 40-50 parts of blue-green algae; and further adding 80-100 parts by weight of a beef extract peptone agar culture medium for culture. By putting the microbial water purifying agent disclosed by the invention into a weever farming pond, the purpose of purifying the water in the farming pond can be achieved.
Description
Technical field
The present invention relates to a kind of microbial water-purifying agent and preparation method thereof, particularly relate to a kind of microbial water-purifying agent for perch aquaculture water and preparation method thereof.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; special aquaculture industry is developed swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river Puffer, swamp eel, loach have reached the level of large-scale production, define the feature of self.
Perch is subordinate to Perciformes, normal perch subfamily, eurysaline fishes.China coast all has distribution, aboundresources.Perch has the features such as growth is fast, wide adaptability, disease resistance are strong, delicious meat.The nutritive elements such as perch rich in proteins, vitamin A, vitamin B group, calcium, magnesium, zinc, selenium; There is the effect of invigorating the liver and kidney, beneficial taste, preventing phlegm from forming and stopping coughing, have good benefiting action to the people of deficiency of the liver and kindey; Perch also can be controlled threatened abortion, produce the diseases such as few breast, ready-to-be mother and produce women to eat perch be a kind ofly not only mended body, but also the nutritive foodstuff that can not cause overnutrition and cause fat, and is that body-building is enriched blood, the good merchantable brand of invigorating the spleen and benefiting QI and beneficial body Ankang; Also have more copper in perch blood, copper can maintain neural normal function and participate in the Function of the key enzyme of number of substances metabolism, and people's edible perch that copper lacks is supplemented.Perch possesses so many advantage, thus deeply popular to people, is to entertain and a large amount of one dish adopted in diet, possesses the wide market requirement.
Natural perch yields poorly, and fishes for difficulty, only propagates the demand that perch could meet people artificially.Comprise the mixed feed such as the fresh food such as zooplankton, red worm and fish slurry, buoyancy particle propagating in perch process the main bait thrown in artificially.But because feed injected volume is often greater than the food-intake of perch, unnecessary feed and the movement of perch can not get effective process and easily cause deteriorating water quality, if do not implement process to aquaculture water, easily cause the rising of perch sickness rate, survival rate reduction, the problems such as production declining, have a strong impact on cultivation.Conventional settling mode is that certain interval of time carries out changing water treatment, but changing water improves production and handling cost on the one hand, and what also easily cause perch on the other hand is not suitable with symptom.
Summary of the invention
For above shortcomings in existing perch cultural technique, one of technical problem to be solved by this invention is to provide a kind of preparation method of microbial water-purifying agent of perch aquaculture water.
Two of technical problem to be solved by this invention is to provide a kind of microbial water-purifying agent of perch aquaculture water, adopts aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of perch aquaculture water, be made up of following step:
(1) magnetic silicon ball of 40-60 weight part finishing alkyl chain is joined in the water of 200-300 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 200-300 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: blue bacterium (Oscillatoria) 50-80 part of quivering greatly, red germ (Rhodomicrobium) 40-80 part, Nitrosococcus (Nitrosococcus) 40-60 part, nitrated thorn bacterium (Nitrospina) 30-50 part, former iron bacteria (Crenothrix) 30-40 part, Pseudomonas aeruginosa (P.Aeruginosa) 30-40 part, bright bacterium (Luecothrix) 3-6 part, bayesian sulphur bacterium (Beggiatoa) 3-6 part, zooflagellate (Zoomastigna) 40-60 part, worms on a sucker (Suctoria) 40-60 part, Dunaliella salina (Dunal iel la) 40-50 part, blue-green algae (Cyanobacteria) 40-50 part,
(3) cultivate 20-30 hour under adding 80-100 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for perch aquaculture water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3 g, add the ultrasonic 8min of 5ml 2M HCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5 ml 10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5 ml water-dispersions;
(1-3) get above-mentioned dispersion liquid B 1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl 0.5g, agar 2.0g and water 100 ml.
Present invention also offers a kind of microbial water-purifying agent for perch aquaculture water, be prepared from by aforesaid method.
By the microbial water-purifying agent for perch aquaculture water of the present invention, putting into cultivation has in the pond of perch, and water purification agent can sink to the water-settling interface at the bottom of pond, can reach the object of purifying aquaculture Chi Shui.Because the magnetic Nano material that have employed specific surface area large does carrier, this microbial water-purifying agent has and is easy to precipitation, high, the effect stability of purification efficiency and be easy to the advantages such as process, the online treatment of very applicable perch aquaculture water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3 g, add the ultrasonic 8min of 5ml 2M HCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5 ml 10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5 ml water-dispersions;
(1-3) get above-mentioned dispersion liquid B 1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the above-mentioned surface finish C 8 of 60 weight part and C18 alkyl chain is added in the water of 300 weight parts;
(3) under 5 revolutions per seconds of agitation conditions, add the microorganism reagent of 300 weight parts, described microorganism reagent is made up of following weight parts component: quiver greatly blue bacterium (Oscillatoria) 65 parts, red germ (Rhodomicrobium) 60 parts, Nitrosococcus (Nitrosococcus) 50 parts, nitrated thorn bacterium (Nitrospina) 40 parts, former iron bacteria (Crenothrix) 40 parts, Pseudomonas aeruginosa (P.Aeruginosa) 40 parts, bright bacterium (Luecothrix) 5 parts, bayesian sulphur bacterium (Beggiatoa) 5 parts, zooflagellate (Zoomastigna) 50 parts, worms on a sucker (Suctoria) 50 parts, Dunaliella salina (Dunal iel la) 50 parts, blue-green algae (Cyanobacteria) 50 parts
(4) the common beef-protein medium of 90 weight part is added again (by extractum carnis 0.5g, peptone 1g, NaCl 0.5g, agar 2.0g, water 100 ml adopts industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for perch aquaculture water can be obtained.
embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for perch aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g dimethyl-octa base silane is adopted to be coating materials in embodiment 2.
embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for perch aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g octadecyltriethoxy silane is adopted to be coating materials in embodiment 3.
test case:
(4m × 4m × 2 m) are tested, and the perch seedling 50 of about 10cm is thrown in every block pond to get four piece sizes and the identical perch breeding pool of water quality.Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
200 grams of microbial water-purifying agents are splashed respectively in perch breeding pool, after 7 days, water-quality determination is carried out to all perch breeding pools.Water quality measurement according to being GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data as shown in the table.
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Contrast pond |
| Dissolved oxygen (DO), mg/L | 7.1 | 6.6 | 6.5 | 4.9 |
| Chemical oxygen demand (COD) (COD), mg/L | 75 | 89 | 93 | 122 |
| Five-day BOD (BOD 5)，mg/L | 12.4 | 14.7 | 14.9 | 18.4 |
| Ammonia nitrogen (NH 3-N) ，mg/L | 0.10 | 0.15 | 0.16 | 0.27 |
| Nitrite (NO 2-)，mg/L | 0.05 | 0.08 | 0.08 | 0.16 |
| Hydrogen sulfide (H 2S) ，mg/L | 0.14 | 0.24 | 0.22 | 0.36 |
As can be seen from data in table, every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, is more obviously better than contrasting pond.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (4)
1. for a preparation method for the microbial water-purifying agent of perch aquaculture water, it is characterized in that, be made up of following step:
(1) magnetic silicon ball of 40-60 weight part finishing alkyl chain is joined in the water of 200-300 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 200-300 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: blue bacterium 50-80 part of quivering greatly, red germ 40-80 part, Nitrosococcus 40-60 part, nitrated thorn bacterium 30-50 part, former iron bacteria 30-40 part, Pseudomonas aeruginosa 30-40 part, bright bacterium 3-6 part, bayesian sulphur bacterium 3-6 part, zooflagellate 40-60 part, worms on a sucker 40-60 part, Dunaliella salina 40-50 part, blue-green algae 40-50 part
(3) 20-30 hour is cultivated under adding 80-100 weight part beef extract-peptone nutrient agar normal temperature again.
2. as claimed in claim 1 for the preparation method of the microbial water-purifying agent of perch aquaculture water, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. as claimed in claim 2 for the preparation method of the microbial water-purifying agent of perch aquaculture water, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3 g, add the ultrasonic 8min of 5ml 2M HCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5 ml 10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5 ml water-dispersions;
(1-3) get above-mentioned dispersion liquid B 1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. for a microbial water-purifying agent for perch aquaculture water, it is characterized in that: adopt method described in claim 1 and 2 and 3 to be prepared from.
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| CN201310572172.1A CN104278020A (en) | 2013-11-17 | 2013-11-17 | Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278019A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
| CN105884046A (en) * | 2016-07-06 | 2016-08-24 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | Photosynthetic type microbial preparation for aquaculture |
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| CN101185874A (en) * | 2007-10-11 | 2008-05-28 | 复旦大学 | Surface finish C8 alkyl chain magnetic silicon ball and preparing method and application thereof |
| CN101712511A (en) * | 2009-04-21 | 2010-05-26 | 北京普仁生态技术有限公司 | Method for controlling cyanobacterial bloom by utilizing composite strain and trace element |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102765776A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Active biological water purifying agent and preparation method thereof |
| CN102963985A (en) * | 2012-11-30 | 2013-03-13 | 江西鸿吉生物科技有限公司 | Biological preparation for balancing water quality for aquaculture |
-
2013
- 2013-11-17 CN CN201310572172.1A patent/CN104278020A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185874A (en) * | 2007-10-11 | 2008-05-28 | 复旦大学 | Surface finish C8 alkyl chain magnetic silicon ball and preparing method and application thereof |
| CN101712511A (en) * | 2009-04-21 | 2010-05-26 | 北京普仁生态技术有限公司 | Method for controlling cyanobacterial bloom by utilizing composite strain and trace element |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102765776A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Active biological water purifying agent and preparation method thereof |
| CN102963985A (en) * | 2012-11-30 | 2013-03-13 | 江西鸿吉生物科技有限公司 | Biological preparation for balancing water quality for aquaculture |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278019A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
| CN105884046A (en) * | 2016-07-06 | 2016-08-24 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | Photosynthetic type microbial preparation for aquaculture |
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