CN104276665B - A kind of microbial water-purifying agent for snakeheaded fish aquaculture water and its preparation method - Google Patents
A kind of microbial water-purifying agent for snakeheaded fish aquaculture water and its preparation method Download PDFInfo
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- CN104276665B CN104276665B CN201310572195.2A CN201310572195A CN104276665B CN 104276665 B CN104276665 B CN 104276665B CN 201310572195 A CN201310572195 A CN 201310572195A CN 104276665 B CN104276665 B CN 104276665B
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 37
- 238000009360 aquaculture Methods 0.000 title claims abstract description 24
- 230000000813 microbial effect Effects 0.000 title claims abstract description 24
- 239000012629 purifying agent Substances 0.000 title claims abstract description 24
- 244000144974 aquaculture Species 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 19
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 19
- 239000010703 silicon Substances 0.000 claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 241000223782 Ciliophora Species 0.000 claims abstract description 6
- 241000192700 Cyanobacteria Species 0.000 claims abstract description 6
- 241000192147 Nitrosococcus Species 0.000 claims abstract description 6
- 239000001888 Peptone Substances 0.000 claims abstract description 6
- 241000192707 Synechococcus Species 0.000 claims abstract description 6
- 241000863000 Vitreoscilla Species 0.000 claims abstract description 6
- 241000195493 Cryptophyta Species 0.000 claims abstract description 4
- 241000295146 Gallionellaceae Species 0.000 claims abstract description 4
- 241000223785 Paramecium Species 0.000 claims abstract description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000005864 Sulphur Substances 0.000 claims abstract description 4
- 235000015278 beef Nutrition 0.000 claims abstract description 4
- 239000006916 nutrient agar Substances 0.000 claims abstract description 4
- 241000684093 bacterium 1-5 Species 0.000 claims abstract 2
- 239000006185 dispersion Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 13
- 239000011248 coating agent Substances 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 239000002122 magnetic nanoparticle Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 6
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 6
- 239000004005 microsphere Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 4
- 229910000077 silane Inorganic materials 0.000 claims description 4
- FZMJEGJVKFTGMU-UHFFFAOYSA-N triethoxy(octadecyl)silane Chemical compound CCCCCCCCCCCCCCCCCC[Si](OCC)(OCC)OCC FZMJEGJVKFTGMU-UHFFFAOYSA-N 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical class [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 3
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 235000011091 sodium acetates Nutrition 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 235000013305 food Nutrition 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241001141454 Crenothrix Species 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 241000862991 Leptothrix <Bacteria> Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001495394 Nitrosospira Species 0.000 description 2
- 241000192497 Oscillatoria Species 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000894100 Tetraselmis chuii Species 0.000 description 2
- 241000190807 Thiothrix Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 241000256844 Apis mellifera Species 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- 241001597062 Channa argus Species 0.000 description 1
- 241000256135 Chironomus thummi Species 0.000 description 1
- 241000252185 Cobitidae Species 0.000 description 1
- 240000009087 Crescentia cujete Species 0.000 description 1
- 235000005983 Crescentia cujete Nutrition 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 235000009797 Lagenaria vulgaris Nutrition 0.000 description 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 1
- 241001327110 Macrobrachium rosenbergii Species 0.000 description 1
- 241000646357 Monopterus cuchia Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000238413 Octopus Species 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 241000736919 Pelodiscus sinensis Species 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- 241000241413 Propolis Species 0.000 description 1
- 241000404975 Synchiropus splendidus Species 0.000 description 1
- 241000270708 Testudinidae Species 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 235000013871 bee wax Nutrition 0.000 description 1
- 239000012166 beeswax Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 229940069949 propolis Drugs 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/346—Iron bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Ecology (AREA)
- Botany (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention discloses a kind of microbial water-purifying agent for snakeheaded fish aquaculture water and its preparation method. Described preparation method: the magnetic silicon ball of 20-40 weight part surface finish C 8 and C18 alkyl chain is joined in the water of 200-400 weight part; Add 80-100 weight part microorganism reagent: Synechococcus 60-80 part, greatly quiver blue bacterium 60-80 part, Nitrosococcus 10-30 part, nitrosification leaf bacterium 10-30 part, iron bacteria 10-20 part, fine send out bacterium 10-20 part, send out sulphur bacterium 1-5 part, Vitreoscilla 1-5 part, paramecium 30-70 part, ciliate 30-70 part, flat algae 30-50 part, blue-green algae 30-50 part; Add 50-90 weight part beef extract-peptone nutrient agar again to cultivate. By the microbial water-purifying agent of the present invention, drop in snakeheaded fish cultivation pond, the object of purifying aquaculture Chi Shui can be reached.
Description
Technical field
The present invention relates to a kind of microbial water-purifying agent and its preparation method, particularly relate to a kind of microbial water-purifying agent for snakeheaded fish aquaculture water and its preparation method.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; make special aquatic products aquaculture development swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river, swamp eel, loach have reached the level of large-scale production, define the feature of self. Snakeheaded fish, another name snakehead, snakeheaded fish, calabash shell serving as a dipper fish, its natural disposition is violent, and reproductivity is strong, and appetite is very big, often can eat up other all fish in certain lake or pond. Before 2,000 years just by " Shennong Bencaojing " and stone honey, honeybee, beeswax (propolis), oyster, tortoise plastron, mulberry tick octopus, that extra large clam, clam, carp etc. are classified as worm fish is top grade. Snakeheaded fish is Chinese's " in dish delicacies ".
Snakeheaded fish natural disposition is violent, the seed staple food of body length less than 3 centimetres scratches sufficient class, cladocera and midge larva etc., the seed of long less than 3-8 centimetre of body is taking the larva of aquatic insect, tadpole, shrimp, prelarva etc. as food, the adult fish that body length is more than 20 centimetres is then taking various small fish and frog as prey, nearly 20,000 jin of the per mu yield of snakeheaded fish, 10 times of four large Chinese carps, and price be also four large Chinese carps twice about, dark liking by raiser. Under Artificial feeding conditions, mainly throw in animal bait, when animal feedstuff is not enough, also can taking artifical compound feeds such as soya-bean cake, dish cake, fish meal as food. But in the process propagated artificially, owing to the density of cultivation is considerably beyond the level of nature, the movement of unnecessary bait and snakeheaded fish cannot be processed by water body, cause water body contaminated, dissolved oxygen declines, ammonia nitrogen index raises, and the growth of snakeheaded fish is had a negative impact, and then affects culture benefit.
In current cultural technique, because aquaculture water cannot be carried out enforcement process, it is easy to cause the rising of snakeheaded fish sickness rate, survival rate to reduce, the problems such as output decline. Conventional settling mode carries out interval for some time changing water treatment, but change water and improve production cost on the one hand, and what also easily cause snakeheaded fish on the other hand is not suitable with symptom.
Summary of the invention
For above shortcomings in existing cultural technique, one of technical problem to be solved by this invention is to provide the preparation method of the microbial water-purifying agent of a kind of snakeheaded fish aquaculture water.
The two of technical problem to be solved by this invention are to provide the microbial water-purifying agent of a kind of snakeheaded fish aquaculture water, adopt aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of snakeheaded fish aquaculture water, it is made up of following step:
(1) magnetic silicon ball of 20-40 weight part finishing alkyl chain is joined in the water of 200-400 weight part;
(2) 2-10 turn/second agitation condition under, add 80-100 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Synechococcus (Synechococcus) 60-80 part, indigo plant of quivering greatly bacterium (Oscillatoria) 60-80 part, Nitrosococcus (Nitrosococcus) 10-30 part, nitrosification leaf bacterium (Nitrosolobus) 10-30 part, iron bacteria (Crenothrix) 10-20 part, fine bacterium (Leptothrix) 10-20 part, send out sulphur bacterium (Thiothrix) 1-5 part, Vitreoscilla (Vitreoscilla) 1-5 part, paramecium (Paramoecium) 30-70 part, ciliate (Ciliate) 30-70 part, flat algae (TetraselmisChui) 30-50 part, blue-green algae (Cyanobacteria) 30-50 part,
(3) cultivate 20-30 hour under adding 50-90 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for snakeheaded fish aquaculture water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is surface finish C 8 and the magnetic silicon ball of C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used3O4Magnetic nano-particle: by 1 gram of FeCl3��6H2O, is dissolved in 30ml ethylene glycol, adds 4 grams of anhydrous sodium acetates, and temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe3O4Magnetic nano-particle;
(1-2) by above-mentioned Fe3O4Magnetic nano-particle washes with water, removes water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, remove green clear liquid with magnet separation again, then use 10ml water-dispersion, then collect particle with magnet, remove the clear liquid of light green, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again with obtaining dispersion liquid B after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is 0.1g dimethyl-octa base silane and the mixture of 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g and water 100ml.
Present invention also offers a kind of microbial water-purifying agent for snakeheaded fish aquaculture water, it is prepared from by aforesaid method.
By the microbial water-purifying agent being used for snakeheaded fish aquaculture water of the present invention, putting into cultivation has in the pond of snakeheaded fish, and water purification agent can sink to the water at the bottom of pond-face, settling boundary, can reach the object of purifying aquaculture Chi Shui. Magnetic Nano material owing to have employed specific surface area big does carrier, and this microbial water-purifying agent has and is easy to precipitation, purification efficiency height, effect stability and is easy to the advantages such as process, is applicable to very much the online treatment of snakeheaded fish aquaculture water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used3O4Magnetic nano-particle: by 1 gram of FeCl3��6H2O, is dissolved in 30ml ethylene glycol, adds 4 grams of anhydrous sodium acetates, and temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe3O4Magnetic nano-particle;
(1-2) by above-mentioned Fe3O4Magnetic nano-particle washes with water, removes water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, remove green clear liquid with magnet separation again, then use 10ml water-dispersion, then collect particle with magnet, remove the clear liquid of light green, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again with obtaining dispersion liquid B after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is 0.1g dimethyl-octa base silane and the mixture of 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the 40 above-mentioned surface finish C 8s of weight part and C18 alkyl chain is added in the water of 400 weight parts;
(3) under 8 turns of/second agitation conditions, add the microorganism reagent of 100 weight parts, described microorganism reagent is made up of following weight parts component: Synechococcus (Synechococcus) 80 parts, quiver greatly blue bacterium (Oscillatoria) 70 parts, Nitrosococcus (Nitrosococcus) 30 parts, nitrosification leaf bacterium (Nitrosolobus) 20 parts, iron bacteria (Crenothrix) 20 parts, a fine bacterium (Leptothrix) 15 parts, send out sulphur bacterium (Thiothrix) 5 parts, Vitreoscilla (Vitreoscilla) 3 parts, paramecium (Paramoecium) 50 parts, ciliate (Ciliate) 50 parts, 40 parts, flat algae (TetraselmisChui), blue-green algae (Cyanobacteria) 40 parts,
(4) the 80 common beef-protein mediums of weight part are added again (by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g, water 100ml adopts the industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for snakeheaded fish aquaculture water can be obtained.
Embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for snakeheaded fish aquaculture water, it is different that difference is only in step (1-4) coating materials. Embodiment 2 adopt 0.3g dimethyl-octa base silane be coating materials.
Embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for snakeheaded fish aquaculture water, it is different that difference is only in step (1-4) coating materials. Embodiment 3 adopt 0.3g octadecyltriethoxy silane be coating materials.
Test case:
Getting four piece sizes and the completely identical snakeheaded fish breeding pool (2m �� 2m �� 1.5m) of water quality is tested, the snakeheaded fish seedling 15 of about 10cm is thrown in every block pond. Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
By microbial water-purifying agent according to 10 grams/meter3Ratio carry out full pool spilling head, after 7 days, all snakeheaded fish breeding pools are carried out water quality mensuration. The foundation of water quality measurement is GB11607-89 People's Republic of China (PRC) national standard water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, and testing index and data are as shown in the table.
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparison pond |
| Dissolved oxygen (DO), mg/L | 7.5 | 6.7 | 6.8 | 3.9 |
| Chemical oxygen demand (COD) (COD), mg/L | 67 | 81 | 85 | 122 |
| Five-day BOD (BOD5), mg/L | 12.1 | 14.1 | 13.5 | 18.1 |
| Ammonia nitrogen (NH3-N), mg/L | 0.22 | 0.27 | 0.27 | 0.33 |
| Nitrite (NO2��), mg/L | 0.31 | 0.52 | 0.61 | 1.05 |
| Hydrogen sulfide (H2S), mg/L | 0.22 | 0.34 | 0.35 | 0.42 |
By data in table it may be seen that every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, more obviously it is better than comparison pond.
The above; it is only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention. Therefore, protection scope of the present invention should be as the criterion with the protection domain that claim book limits.
Claims (4)
1. the preparation method for the microbial water-purifying agent of snakeheaded fish aquaculture water, it is characterised in that, it is made up of following step:
(1) magnetic silicon ball of 20-40 weight part finishing alkyl chain is joined in the water of 200-400 weight part;
(2) 2-10 turn/second agitation condition under, adding 80-100 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Synechococcus 60-80 part, greatly quiver blue bacterium 60-80 part, Nitrosococcus 10-30 part, nitrosification leaf bacterium 10-30 part, iron bacteria 10-20 part, fine send out bacterium 10-20 part, send out sulphur bacterium 1-5 part, Vitreoscilla 1-5 part, paramecium 30-70 part, ciliate 30-70 part, flat algae 30-50 part, blue-green algae 30-50 part;
(3) 20-30 hour is cultivated under adding 50-90 weight part beef extract-peptone nutrient agar normal temperature again.
2. the preparation method of the microbial water-purifying agent for snakeheaded fish aquaculture water as claimed in claim 1, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. the preparation method of the microbial water-purifying agent for snakeheaded fish aquaculture water as claimed in claim 2, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is surface finish C 8 and the magnetic silicon ball of C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used3O4Magnetic nano-particle: by 1 gram of FeCl3��6H2O, is dissolved in 30ml ethylene glycol, adds 4 grams of anhydrous sodium acetates, and temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe3O4Magnetic nano-particle;
(1-2) by above-mentioned Fe3O4Magnetic nano-particle washes with water, removes water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, remove green clear liquid with magnet separation again, then use 10ml water-dispersion, then collect particle with magnet, remove the clear liquid of light green, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again with obtaining dispersion liquid B after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is 0.1g dimethyl-octa base silane and the mixture of 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. the microbial water-purifying agent for snakeheaded fish aquaculture water, it is characterised in that: adopt method according to any one of claim 1-3 to be prepared from.
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