CN103992187A - Biological bacterial fertilizer for preventing and treating moss in water body and preparation method thereof - Google Patents
Biological bacterial fertilizer for preventing and treating moss in water body and preparation method thereof Download PDFInfo
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- CN103992187A CN103992187A CN201410266595.5A CN201410266595A CN103992187A CN 103992187 A CN103992187 A CN 103992187A CN 201410266595 A CN201410266595 A CN 201410266595A CN 103992187 A CN103992187 A CN 103992187A
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Abstract
The invention provides a biological bacterial fertilizer for preventing and treating moss in a water body and a preparation method thereof. The biological bacterial fertilizer is prepared from sodium humate, amino acid raw powder, Bacillus subtilis and sodium silicate. The sodium humate dissolved in water can turn water into light black or black and perform the function of shielding sunlight, so that the moss or mud cake on the tank bottom dies due to loss of lighting; the free amino acids and silicic acid can quickly promote the growth of the beneficial algae, thereby quickly cultivating the algae and enriching the water; and the Bacillus subtilis in the biological bacterial fertilizer can generate multiple enzymatic systems to decompose and utilize organic matters in the moss, mud cake, residual bait and manure in the culture water body, provides nutrients for algae growth, forms favorable diatom water color and lowers the transparency of the water body. The biological bacterial fertilizer has multiple biological water enrichment functions of killing the moss, decomposing the organic matters and quickly cultivating the beneficial algae. The biological bacterial fertilizer has the advantages of no environmental pollution, no public nuisance, low cost, wide application range and the like, is biodegradable and is simple to operate.
Description
Technical field
The present invention relates to a kind of bio-bacterial manure that prevents and treats water body moss and preparation method thereof, belong to technical field of aquaculture.
Background technology
Moss also claims blue mud tongue, is the common name of several silkweeds such as water silk floss, double star algae, flap algae.The cultivation of moss prawn crab can have a negative impact: in the floating water of moss silk, affect shrimp crab growth and ingest, if be strayed into wherein, being often wound, making its limitation of activity, the soft shell shrimp crab after shelling and physique are dead because not escaping compared with weak; Moss absorbs the nutritive substances such as the nitrogen phosphorus potassium in water body, consumes nutrient, makes water quality variation; Affect the absorption of planktonic organism to luminous energy, planktonic organism is reduced, hinder the raising of water temperature and dissolving in of oxygen; Moss and pasture and water are intertwined, and directly affect the growth of pasture and water; At high temperature season, the moss death of rising in pond, it produces hazardous and noxious substances in decomposition course, and as hydrogen sulfide, ammonia-state nitrogen etc., very easily cause shrimp, crab, shellfish anoxic, are poisoned to death.
At present, there is the measure of moss in prevention culturing pool: after annual aquaculture product has been gathered in the crops, Jiang Chishui drains, and cuts out the clear pond of top layer mud sterilize with dozer; While receiving liquid manure pond spring, look the pond liquid manure degree rational application of fertilizer, receiving water can not be shallow, and fertilizer will dissolve, and full pool spilling head is even, avoids fertilizer to sink to the bottom.In culturing pool, there is the treatment process after moss: in the time that culturing pool is not put fishery products seedling in a suitable place to breed and occurred a large amount of moss, every mu of water surface will sprinkle a few kilogram of raw lime moss is killed, and then drains Chi Shui, again Na Shuifeichi.Put fishery products seedling in a suitable place to breed and while there is a small amount of moss, raised as far as possible pond water water level when culturing pool, breeding Micro Algae, grows fat Chi Shui, suppresses moss breeding.Have the pool of moss, be generally difficult to rich water, when fertilising, fertilizer has all absorbed to moss, causes algae long not, more fertilising, moss looks faster, water is thin, the end, is poor, can cause shrimp stress, be lowered into motility rate.When pond put fishery products seedling in a suitable place to breed and moss in pond when amount reproduction, use the medicines such as prometryn, moss are clean, copper sulfate, sodium pentachlorophenate to remove.But copper sulfate use is excessive, is easy to produce poisoning, usage quantity is few, inoperative again; Prometryn is not clear to the aquatic animal mechanism of action, bans use of.In addition, also can adopt powership to travel and in pond, produce turbid wave, or the yellow mud of splashing, make pool water become muddy, reduce water transparence, suppress moss growth, and adopt pole or special rake, net the instrument such as to drag for manually to skim, exist like this hand labor intensity large.Liu Guangmou etc. disclose a kind of method of preventing and treating of lichen in sea water culture pond in patent 200910014398.3, utilize the ocean macro of hanging culture to keep the sun off, and have effectively suppressed moss growth.But its applicable sea farming.The gorgeous rosy clouds of human relations disclose a kind of for removing the composition and method of making the same of moss in patent 201210260062.7, and described composition is biological algal control element, plant ash and clinoptilolite powder; Fan Jixiu etc. disclose one and have killed tongue algicide by 2-methylthio group-4 in patent 201210449647.3,6-bis-(ethylamino)-1,3,5-triazine, dodecyl benzyl dimethyl ammonium chloride, three kinds of material compositions of isothiazolinone, it can be killed moss at the bottom of pond, but the moss of killing, or corrupt and pollute substrate, not degraded targetedly at the bottom of pond.Up to the present, also do not have one both to have control moss, the patent again with the product of rich water discloses.
Summary of the invention
High with operation control moss cost in order to overcome prior art Chinese traditional medicine, and easily breed variety is worked the mischief and had side effects, manually suppress, remove moss hand labor intensity large, after killing moss, substrate degenerates, and also there is not one both to there is control moss, there is again the technical products of rich water function, the invention provides a kind of bio-bacterial manure and preparation method who had not only there is control water body moss but also there is quick rich water function.This bio-bacterial manure is easy to use, simple to operate, and use range is wide, and cost is low, and can prevent and treat moss, decomposes dead moss in cultivating pool, organic matter, a kind of multifunctional bio rich water product that can the useful algae of fast culture.
Another object of the present invention is to provide the preparation method of this bio-bacterial manure.
To achieve these goals, the technical solution adopted in the present invention is: a kind of for preventing and treating the bio-bacterial manure of water body moss, it is characterized in that, the raw material of preparing this biotechnological formulation is by weight: sodium humate 600-800 part, the former powder 200-400 of amino acid part, subtilis 5-10 part, water glass 1-5 part;
In order to achieve the above object, of the present inventionly it is characterized in that for preventing and treating the preparation method of bio-bacterial manure of water body moss, comprise the steps:
, raw material premix: it is even that 1-5 part water glass and 5-10 part subtilis first carry out premix;
, stirring and evenly mixing: in stirred pot, add sodium humate 600-800 part successively, the former powder 200-400 of amino acid part, the water glass having mixed in advance and subtilis powder, open stir 20-30 minute, after mixing, pulverized 100 orders, can pack.
Wherein, step
its gemma content of described subtilis powder is not less than 20,000,000,000/gram.
Wherein, step (2) the described former powder of amino acid be that free aminoacid content is not less than 30%.
Wherein, the water glass of step (1), in (2) and sodium humate from the market outsourcing obtain.Wherein, the effective content in commercial sodium humate is not less than 85%.
In step
in subtilis buyable, also can adopt prior art cultivate, the invention provides following methods:
The preparation of subtilis bacterium powder
Take out culture presevation pipe, draw flat board with LB solid medium and recover, cultivate 48 hours for 37 DEG C.Under flat board, in 50 milliliters of LB substratum of the single colony inoculation of picking, in incubator, 37 DEG C of concussions are cultivated 24 hours.Seed adopts 5% inoculum size, be seeded to that in the large triangular flask of 5L, (liquid amount is 2L, LB substratum), 20-28 hour is cultivated in 37 DEG C of concussions, detect its bacterial concentration, viable bacteria content exceedes 6,000,000,000/milliliter, can be used as subtilis liquid-spawn inoculation to 1000L fermention medium, inoculum size is 5%, open and stir 120r/min, air flow 1:0.8 (liquid/gas), cultivate 16-24 hour for 37 DEG C, treat that gemma content is not less than 25,000,000,000/milliliter, can finish fermentation, Plate Filtration dewaters, remaining thalline adds light calcium carbonate, regulate that bacterium is dense is 20,000,000,000/gram, pulverize 100 orders and obtained genus bacillus bacterium powder.Wherein said LB substratum: peptone 5g, extractum carnis 3g, sodium-chlor 5g, water 1000mL, pH7.2, adds agar 2% in solid medium.Wherein said genus bacillus liquid bacterium culture medium: glucose 10g/L, Semen Maydis powder 10 g/L, bean cake powder 30 g/L, magnesium sulfate 0.5 g/L, manganous sulfate 0.5 g/L, SODIUM PHOSPHATE, MONOBASIC 0.5 g/L, Sodium phosphate dibasic 2.3 g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.
Bio-bacterial manure of the present invention, in the time of the moss preventing and treating in cultivating pool, applies in pond with the amount of 2000-4000 gram/acre, and the method that applies, for changing water full pool spilling head, is driven oxygenating machine and stirred water more than 15 minutes, and humic acid is wherein stirred in whole pond, stir evenly.
In bio-bacterial manure raw material composition of the present invention, contain sodium humate.Sodium humate applies after water body, first plays interception: in sodium humate, contain Ulmic acids etc. and have melanochrome, molten water is black, and shield lights reduces water transparency; Next has and helps fertile detoxification: in sodium humate, contain SODLUM FULVATE, the multiple functional group such as phenolic hydroxyl group, the carboxyl Adsorption of Heavy Metals of SODLUM FULVATE, nitrite, ammonia-state nitrogen, the nutritive elements such as hydrogen sulfide and slowly-releasing organic matter, trace element, utilization ratio is high and long-acting, prevents algae and the growth of mushroom explosive type and withers away, stabilizing water quality.
In biotechnological formulation raw material composition of the present invention, contain subtilis, subtilis is the organism in degrading cultivation pond rapidly, comprise mud at the bottom of the movement of fish, remaining feed, planktonic algae corpse and pond, make it to generate the inorganic salts such as nitrate, phosphoric acid salt, vitriol, effectively reduce the content of COD, BOD in water, make ammonia nitrogen ((NH4+-N) and nitrous acid nitrogen (NO2--N), sulfide concentration reduction in water body, thereby effectively improve water quality, avoid the deposition of organism at culturing pool, maintain good cultivation water area ecological environment.Li Zhuojia etc. carry out decomposing, purifying with genus bacillus to the mud in seabed stores, fishpond, receive good effect.Zhang Qing etc. add 1 time taking genus bacillus as main complex microbial inoculum every 25d in tilapia aquaculture water, can obviously improve condition of water quality, effectively reduce ammonia nitrogen and nitrite, build good water colour, promote the growth of tilapia.Subtilis of the present invention is the meticulous screening through for many years, can breed rapidly in the pond bottom compared with hypoxemia, produce a large amount of amylase, proteolytic enzyme, residual bait, dead moss in energy efficient degradation pond, mud skin, ight soil and other organic waste, be converted into low molecular organic acids, nitrate, the inorganic salts such as phosphoric acid salt, vitriol, for the plant planktons such as algae provide nutrition, reduces bed mud alluvial.Accelerate pond material cycle and flow of energy, set up the balance of plant plankton and natural probiotics and come stabilizing water quality and water colour.
In biotechnological formulation raw material composition of the present invention, contain a large amount of total free aminoacidss, Xu Nins etc. have studied the utilize characteristic research of marine microalgae to total free aminoacids, think that total free aminoacids can directly be utilized by algae, transform fast, and promote from practice, can play algae photosynthesis low temperature and train fast algae rich water; The front steady water of overcast and rainy, the change of weather; After algae, fast culture plays good water colour.
In biotechnological formulation raw material of the present invention composition, contain water glass, for pond waters provides soluble silicon salt, can greatly improve the reproduction speed of useful algae diatom, for it can form advantage algae kind in planktonic algae, and form good water colour and create favorable conditions.
The invention has the beneficial effects as follows: bio-bacterial manure of the present invention applies behind pond, start after stirring, sodium humate is dissolved in water body uniformly, Ulmic acids in sodium humate makes the aobvious somber of water colour or black, water transparency becomes about 30-80cm from see the bottom (100-150cm) very soon, the moss of bottom and mud skin be due to by black shading like this, and lose light source, death in ensuing 1 to 2 day; And free amino acid and water glass in bio-bacterial manure now directly utilized by algae, transform soon, and promote algae photosynthesis, can play low temperature and train fast algae rich water; And water glass can improve the reproduction speed of useful algae diatom greatly, for it can form advantage algae kind in planktonic algae, and form good water colour and create favorable conditions.
And in subsequently one to two day, subtilis in bio-bacterial manure is activated, start the efficient amylase that produces, residual bait in proteasome degradation pond, dead moss, mud skin, ight soil and other organic waste are converted into low molecular organic acids, nitrate, the inorganic salts such as phosphoric acid salt, vitriol, for the plant planktons such as algae provide nutrition, can greatly improve the reproduction speed of useful algae, accelerate pond material cycle and flow of energy, set up the balance of planktonic algae and probiotics and carry out stabilizing water quality, finally form good diatom water colour.
If the clear thorough pond seeing the bottom tongue not green for a long time, apply after this bio-bacterial manure, too due to the interception of humic acid, and free amino acid and silicic acid can promote fast the growth of useful algae and play quick training algae rich water, reduce water transparency, thereby can prevent the growth of moss.
Bio-bacterial manure of the present invention discloses one first both can prevent and treat water body moss, and rich water fast again decomposes organicly, promotes the multifunctional bio bacterial manure of useful algae growth.The effect of the control moss of bio-bacterial manure of the present invention far wins copper sulfate, potassium permanganate, and prometryns etc., not only can be used for cultivating pool, also can be used for the control of the moss in the seawater of outbeach.Environmentally safe, toxicological harmless, biodegradable, low cost, nuisanceless, simple to operate, the advantage such as purposes is wide.This technology tallies with the national condition and aquaculture technology developing direction, and in aquaculture, particularly shrimp crayfish culture has broad application prospects.
Below in conjunction with the effect of test explanation bio-bacterial manure control of the present invention moss.
One,
mud skin is processed in this bio-bacterial manure
1, test site: sea-gull island, Panyu District of Guangzhou City; Breed variety: Penaeus vannamei; Area: eight mu; Culture style: Tu Tang raises together with; Put number of days in a suitable place to breed: 0 day;
2, problem is described: put before seedling, water is thin, at the bottom of can seeing pond, has a large amount of mud skins at the bottom of pond;
3, case study: have the pool of mud skin, be generally difficult to rich water, when fertilising, fertilizer has absorbed all to mud skin, cause algae grow not, if so just put seedling, can cause shrimp seedling stress, be lowered into motility rate;
4, solution: every mu uses 2000 grams of this bio-bacterial manures;
5, result: after this bio-bacterial manure is used, water colour is light/dark balance, and transparency becomes 60cm from 120cm, and the 3rd day, a large amount of mud skins floated on the surface of the water.Within the 3rd day and the 4th day, can obviously see that algae has grown, water colour is yellow-green colour;
6, sum up: this bio-bacterial manure can reduce the transparency of water, at the bottom of block sunlight directly shines on pond.Mud skin, owing to lacking illumination, cannot carry out photosynthesis, dead floating.After death, the algae in the pool, can Fast Growth because not had nutrient competition object for mud skin.The subtilis of this bio-bacterial manure simultaneously can be decomposed mud skin, promotes useful algae growth, prevents that water quality from degenerating.
Two, moss is processed in this bio-bacterial manure
1, test site: sea-gull island, Panyu District of Guangzhou City; Breed variety: Penaeus vannamei; Area: 16 mu; Culture style: Tu Tang raises together with; Put number of days in a suitable place to breed: 20 days;
2, problem is described: in earlier stage, Changes in weather is fast in cultivation, algae, and water is thin, at the bottom of can seeing pond, has a large amount of mosses at the bottom of several days after-bays;
3, case study: have the pool of moss, be generally difficult to rich water, when fertilising, fertilizer has absorbed all to mud skin, cause algae grow not, water is thin, the end, is poor, can cause shrimp stress, be lowered into motility rate;
4, solution: every mu uses 4000 grams of this bio-bacterial manures;
5, result: after this bio-bacterial manure is used, water colour is black, and transparency becomes 40cm from 110cm, and second day, a large amount of mosses became lark, black by green.Within the 3rd day and the 4th day, can obviously see that algae has grown, water colour is yellow-green colour;
6, sum up: this bio-bacterial manure can reduce the transparency of water, at the bottom of block sunlight directly shines on pond.Moss, owing to lacking illumination, cannot carry out photosynthesis, death.After death, the algae in the pool, because not had nutrient competition object, absorbs amino acid energy Fast Growth to moss.The subtilis of this bio-bacterial manure simultaneously can be decomposed moss, promotes useful algae growth.
Embodiment
Substantive distinguishing features of the present invention can be embodied from the following examples, but only conduct explanation of these embodiment, instead of limit the invention.
Embodiment 1
The raw material of preparing this bio-bacterial manure is by weight: 700 parts of sodium humates, 300 parts, the former powder of amino acid, 5 parts of subtilises, 2 parts of water glass.
The preparation method of the bio-bacterial manure of this control water body moss, comprises the steps:
, raw material premix: it is even that 2 parts of water glass and 5 parts of subtilises first carry out premix;
, stirring and evenly mixing: in stirred pot, add 700 parts of sodium humates successively, 300 parts, the former powder of amino acid, the water glass having mixed in advance and subtilis powder, open stir 20-30 minute, after mixing, pulverized 100 orders, can pack.
Embodiment 2
The raw material of preparing this bio-bacterial manure is by weight: 600 parts of sodium humates, 400 parts, the former powder of amino acid, 10 parts of subtilises, 3 parts of water glass.
The preparation method of the bio-bacterial manure of this control water body moss, comprises the steps:
, raw material premix: it is even that 3 parts of water glass and 10 parts of subtilises first carry out premix;
, stirring and evenly mixing: in stirred pot, add 600 parts of sodium humates successively, 400 parts, the former powder of amino acid, the water glass having mixed in advance and subtilis powder, open stir 20-30 minute, after mixing, pulverized 100 orders, can pack.
Claims (5)
1. for preventing and treating a bio-bacterial manure for water body moss, it is characterized in that, the raw material of preparing this bio-bacterial manure is by weight: sodium humate 600-800 part, the former powder 200-400 of amino acid part, subtilis 5-10 part, water glass 1-5 part.
2. according to claim 1ly it is characterized in that for preventing and treating the preparation method of bio-bacterial manure of water body moss, comprise the steps:
(1), raw material premix: it is even that 1-5 part water glass and 5-10 part subtilis first carry out premix;
(2), stirring and evenly mixing: in stirred pot, add sodium humate 600-800 part successively, the former powder 200-400 of amino acid part, the water glass having mixed in advance and subtilis powder, open stir 20-30 minute, after mixing, pulverized 100 orders, can pack.
3. according to claim 1ly it is characterized in that for preventing and treating the bio-bacterial manure of water body moss, described subtilis, its gemma content is not less than 20,000,000,000/gram.
4. according to claim 1ly it is characterized in that for preventing and treating the bio-bacterial manure of water body moss, the former powder of described amino acid, its free aminoacid content is not less than 30%.
5. according to claim 1ly it is characterized in that for preventing and treating the bio-bacterial manure of water body moss, the effective content in described sodium humate is not less than 85%.
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| CN110698258A (en) * | 2019-10-29 | 2020-01-17 | 徐州快邦生物科技开发有限公司 | Medicine for removing rice field moss and preparation method thereof |
| CN111187731A (en) * | 2019-11-29 | 2020-05-22 | 新洋丰农业科技股份有限公司 | Biological bottom-improving algae-culturing water quality improving microbial inoculum and preparation method thereof |
| CN112794458A (en) * | 2020-12-23 | 2021-05-14 | 南京升鳌生物环保科技有限公司 | Moss inhibitor for biological purifying water body and its preparing method and use |
| CN114617135A (en) * | 2022-03-14 | 2022-06-14 | 中国林业科学研究院林产化学工业研究所 | Biological agent with moss deactivating effect and preparation method and application thereof |
| CN114617135B (en) * | 2022-03-14 | 2024-04-26 | 中国林业科学研究院林产化学工业研究所 | Biological agent with green removing function and preparation method and application thereof |
| CN114751793A (en) * | 2022-04-25 | 2022-07-15 | 武汉都兴隆农业科技有限公司 | New effect of amino acid nitrogen fertilizer in inhibiting growth of moss in shrimp farming field and preparation method thereof |
| CN115895935A (en) * | 2022-08-08 | 2023-04-04 | 上海海洋大学 | Bacillus subtilis and application thereof in prevention and control of lichen |
| CN115895935B (en) * | 2022-08-08 | 2024-02-09 | 上海海洋大学 | Bacillus subtilis and application thereof in moss control |
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