CN103936174B - Biological agent for preventing and treating mosses in water body and preparation method of biological agent - Google Patents

Biological agent for preventing and treating mosses in water body and preparation method of biological agent Download PDF

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CN103936174B
CN103936174B CN201410205077.2A CN201410205077A CN103936174B CN 103936174 B CN103936174 B CN 103936174B CN 201410205077 A CN201410205077 A CN 201410205077A CN 103936174 B CN103936174 B CN 103936174B
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water body
biotechnological formulation
biological agent
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CN103936174A (en
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徐秀良
王登广
刘龙
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China Railway Construction Ecological Environment Co ltd
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CHINA RAILWAY CONSTRUCTION ECO-ENVIRONMENTAL DESIGN AND RESEARCH Co Ltd
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Abstract

The invention provides a biological agent for preventing and treating mosses in a water body and a preparation method of the biological agent. The biological agent is prepared from the following raw materials: food-grade brilliant blue, bacillus subtilis, yeast and sodium silicate. The brilliant blue in the biological agent provided by the invention is dissolved into water to enable the water to become light blue, so that the sunlight is shielded, and mosses and mud cakes at the bottom of a pool are dead of losing illumination; bacillus subtilis and yeast in the biological agent generate various enzyme systems to decompose and utilize the mosses, mud cakes and residual feeds in the water body and organic matters in faeces and provide nutrients for the growth of algae, and sodium silicate induces useful algae and diatoms to rapidly growth to form a preponderant algae species. The biological agent plays various biological water regulating roles in killing the mosses, decomposing the organic matters and promoting the growth of the useful algae. The biological agent can be used for preventing and treating mosses in a culture pond, a landscape water body and offshore seawater and has the advantages of no environment pollution and public hazards, biodegradablity, low dosage, simplicity in operation, wide application range, long duration time and the like.

Description

A kind of biotechnological formulation for preventing and treating water body moss and preparation method thereof
Technical field
The present invention relates to and a kind ofly prevent and treat biotechnological formulation of water body moss and preparation method thereof, belong to technical field of aquaculture.
Background technology
Moss, also known as blue mud tongue, is the common name of several silkweeds such as water silk floss, double star algae, flap algae.The cultivation of moss prawn crab can have a negative impact: in the floating water of moss silk, affects the growth of shrimp crab and ingests, if be strayed into wherein, being often wound, making its limitation of activity, the soft shell shrimp crab after shelling and more weak dead because not escaping of physique; Moss absorbs the nutritive substances such as the N P and K in water body, consumes nutrient, makes water degradation; Affect the absorption of planktonic organism to luminous energy, planktonic organism is reduced, hinder the raising of water temperature and dissolving in of oxygen; Moss and pasture and water are intertwined, and directly affect the growth of pasture and water; At high temperature season, moss is risen death in pond, and it produces hazardous and noxious substances in decomposition course, and as hydrogen sulfide, ammonia-state nitrogen etc., very easily cause shrimp, crab, shellfish anoxic, be poisoned to death.
At present, there is the measure of moss in prevention culturing pool: after annual aquaculture product has been gathered in the crops, Jiang Chishui drains, and cuts out the clear pond sterilization of top layer mud with dozer; Receive spring liquid manure pond time, look the pond liquid manure degree rational application of fertilizer, receiving water can not be shallow, and fertilizer will dissolve, and full pool spilling head is even, avoids fertilizer to sink to the bottom.The treatment process after moss is there is: when culturing pool is not put fishery products seedling in a suitable place to breed and occurred a large amount of moss, every mu of water surface will sprinkle a few kilogram of raw lime and be killed by moss, then drains Chi Shui, again Na Shuifeichi in culturing pool.When culturing pool has been put fishery products seedling in a suitable place to breed and occurred a small amount of moss, raise pond water water level as far as possible, breeding Micro Algae, makes Chi Shui grow fat, and suppresses moss breeding.When pond has put fishery products seedling and moss in a suitable place to breed in pond during amount reproduction, the medicines such as prometryn, moss are clean, copper sulfate, sodium pentachlorophenate be used to remove.But copper sulfate use is excessive, be easy to produce poisoning, usage quantity is few, inoperative again; Prometryn is failed to understand the aquatic animal mechanism of action, prohibits the use.In addition, powership also can be adopted to travel and in pond, to produce turbid wave, or yellow mud of splashing, make pool water become muddy, reduce water transparence, suppress moss growth, and adopt pole or special rake, net drags for etc., and instrument manually skims, and there is hand labor intensity so large.Liu Guangmou etc. disclose a kind of prevention and controls of lichen in sea water culture pond in patent 200910014398.3, namely utilize the ocean macro of hanging culture to keep the sun off, and restrained effectively moss growth.But its applicable sea farming.The gorgeous rosy clouds of human relations disclose a kind of composition and method of making the same for removing moss in patent 201210260062.7, described composition is biological algal control element, plant ash and clinoptilolite powder, moss at the bottom of pond can be killed by it, but the moss killed, or corrupt and contaminated sediment at the bottom of pond, does not degrade targetedly.
Summary of the invention
In order to overcome prior art Chinese traditional medicine, to prevent and treat moss cost high with operation, and easily breed variety worked the mischief and have side effects, artificial suppression, to remove moss hand labor intensity large, after killing moss, substrate degenerates, and be confined to the deficiency of scope of sea farming, the invention provides a kind of biotechnological formulation and the preparation method that prevent and treat water body moss.This biotechnological formulation is easy to use, simple to operate, and use range is wide, and can decompose dead moss in cultivating pool, organic, a kind of multifunctional bio water transfer preparation that useful algae can be promoted to grow.
Another object of the present invention is to provide the preparation method of this biotechnological formulation.
To achieve these goals, the technical solution adopted in the present invention is: a kind of biotechnological formulation for preventing and treating water body moss, is characterized in that, the raw material preparing this biotechnological formulation is by weight: food grade light blue 40-70 part, genus bacillus 20-40 part, yeast 5-15 part, water glass 5-15 part;
In order to achieve the above object, the preparation method of the biotechnological formulation for preventing and treating water body moss of the present invention, is characterized in that, comprise the steps:
(1), raw materials pretreatment: after 5-15 part water glass and the pre-mixing of 40-70 part food grade light blue, pulverized 100 orders;
(2), stirring and evenly mixing: add 20-40 part genus bacillus bacterium powder successively in stirred pot, 5-15 part yeast powder, pretreated light blue and water glass powder, open and stir 20-30 minute, can pack after mixing;
(3), double casing: after first being utilized by the biotechnological formulation mixed Soluble Plastic Packing to install by 100 grams/handbag, pack one deck with common plastics more outside, be finished product.
Wherein, the (2) described genus bacillus powder of step is subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus natto, bacillus amyloliquefaciens, bacillus megaterium, a kind of and several mixture in bacillus pumilus; Its spore content is not less than 20,000,000,000/gram.
Wherein, the (2) described yeast of step is yeast saccharomyces cerevisiae, Candida utilis, candida tropicalis, bread yeast, a kind of and several mixture in cereuisiae fermentum, the viable bacteria content of yeast powder is not less than 2,000,000,000/gram.
Wherein, step (1) in water glass and food grade light blue from the market outsourcing obtain.Wherein, the light blue content in commercial food grade light blue is not less than 85%.
Step (2) in genus bacillus, yeast buyable, prior art also can be adopted to cultivate, the invention provides following methods:
The preparation of genus bacillus bacterium powder
Take out culture presevation pipe, draw flat board with LB solid medium and recover, cultivate 48 hours for 37 DEG C.Under flat board in the single colony inoculation of picking 50 milliliters of LB substratum, in incubator, 37 DEG C shake cultivation 24 hours.Seed adopts the inoculum size of 5%, be seeded in the large triangular flask of 5L that (liquid amount is 2L, LB substratum), 20-28 hour is cultivated in 37 DEG C of concussions, detect its bacterial concentration, viable bacteria content is more than 6,000,000,000/milliliter, namely can be used as bacillus liquid body strain inoculation to 1000L fermention medium, inoculum size is 5%, open and stir 120r/min, air flow 1:0.8 (liquid/gas), cultivate 16-24 hour for 37 DEG C, treat that spore content is not less than 25,000,000,000/milliliter, fermentation can be terminated, Plate Filtration dewaters, remaining thalline adds light calcium carbonate, to regulate bacterium dense be 20,000,000,000/gram, pulverize 100 orders and namely obtain genus bacillus bacterium powder.Wherein said LB substratum: peptone 5g, extractum carnis 3g, sodium-chlor 5g, water 1000mL, pH7.2, add agar 2% in solid medium.Wherein said genus bacillus liquid bacterium culture medium: glucose 10g/L, Semen Maydis powder 8 g/L, bean cake powder 25 g/L, magnesium sulfate 0.5 g/L, manganous sulfate 0.5 g/L, SODIUM PHOSPHATE, MONOBASIC 0.5 g/L, Sodium phosphate dibasic 2.3 g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.
The preparation of yeast powder
Take out culture presevation pipe, draw flat board with PDA solid medium and recover, cultivate 48 hours for 30 DEG C.Under flat board in the single colony inoculation of picking 150 milliliters of PDA substratum, in incubator, 30 DEG C shake cultivation 24 hours.Seed adopts the inoculum size of 5%, be seeded in the large triangular flask of 5L that (liquid amount is 2L, PDA substratum), 20-28 hour is cultivated in 30 DEG C of concussions, detect its bacterial concentration, viable bacteria content is more than 1,000,000,000/milliliter, namely can be used as yeast liquid body strain inoculation to 1000L fermention medium, inoculum size is 5%, open and stir 120r/min, air flow 1:0.5 (liquid/gas), cultivate 20-28 hour for 30 DEG C, treat that yeast content is not less than 4,000,000,000/milliliter, fermentation can be terminated, Plate Filtration dewaters, remaining thalline adds light calcium carbonate, to regulate bacterium dense be 2,000,000,000/gram, pulverize 100 orders and namely obtain yeast bacterium powder.Wherein said PDA substratum: potato 200g, sucrose 20g, water 1000mL, add agar 2% in solid medium.Wherein said yeast seed culture medium: glucose 10 g/L, Semen Maydis powder 30g/L, dregs of beans 10g/L, yeast extract paste 3 g/L, peptone 5 g/L, ammonium sulfate 5 g/L, magnesium sulfate 0.5 g/L.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.
Biotechnological formulation of the present invention is when preventing and treating the moss in cultivating pool, apply in pond with the amount of a parcel (100 grams)/mu, the method that applies is for tear outer packaging, the solubility plastics bag of internal packing is not opened, directly the solubility plastics bag of internal packing and biotechnological formulation entirety are placed in the other water of oxygenating machine, drive oxygenating machine and stir water more than 15 minutes, light blue is wherein stirred in whole pond, stir evenly.If landscape water, then add the source of landscape water with the concentration of 0.1 gram of biotechnological formulation/cube water, allow whole water body Homogeneous phase mixing.
Containing food kind level light blue in biotechnological formulation raw material composition of the present invention.Light blue, with red-violet colour particle or the powder of metalluster, odorless, the aqueous solution is in blue, and stable in tartrate, citric acid, alkali resistance is strong, good salt tolerance.Light blue is as foodstuff additive, and use range and usage quantity China " edible additive use hygienic standard " (GB 2760-1996) specify: can be used for color equipment on high candy juice (taste), fruit juice (taste) beverage, soda pop, assembled alcoholic drinks, candy, cake; For green plum, shrimp (taste) sheet, maximum usage quantity 0.025g/kg; For ice-creams, maximum usage quantity 0.022g/kg; For red and green pickled fruit, maximum usage quantity 0.1g/kg, and the light blue of maximum level 70% in biotechnological formulation of the present invention, its content adding pond water to is 0.1g/1000kg water (100 grams of biotechnological formulation/mu ponds, deeply calculate with 1.2 meters of ponds), then its addition is well below the maximum usage quantity of foodstuff additive, is completely safe, harmless.In addition, Jing Shunjie etc. report that light blue is under natural light, and after 9 days, its degradation rate can reach 80%, can not cause water pollution, and this and we are at large pool culture experiment after 10 to 20 days, and it is consistent that the color of the light blue in pond disappears substantially.
Containing genus bacillus in biotechnological formulation raw material composition of the present invention, the organism in the rapid degrading cultivation pond of genus bacillus energy, comprise mud at the bottom of the movement of fish, remaining feed, planktonic algae corpse and pond, make it to generate the inorganic salts such as nitrate, phosphoric acid salt, vitriol, the content of COD, BOD in effective reduction water, ((NH4+-N) and cultured water (NO2--N), sulfide concentration reduce to make the ammonia nitrogen in water body, thus effectively improve water quality, avoid organism in the deposition of culturing pool, maintain good Cultivated water ecotope.Li Zhuojia etc. carry out decomposing, purifying with genus bacillus to the mud in seabed stores, fishpond, receive good effect.Zhang Qing etc. add 1 complex microbial inoculum based on genus bacillus every 25d in tilapia aquaculture water, obviously can improve condition of water quality, effectively reduce ammonia nitrogen and nitrite, build good water colour, promote the growth of tilapia.Genus bacillus of the present invention is through meticulous screening for many years, can breed rapidly in the pond bottom compared with hypoxemia, produce a large amount of amylase, proteolytic enzyme, residual bait in energy efficient degradation pond, dead moss, mud skin, ight soil and other organic waste, be converted into low molecular organic acids, nitrate, the inorganic salts such as phosphoric acid salt, vitriol, for the plant planktons such as algae provide nutrition, reduces bed mud alluvial.Accelerate pond material cycle and flow of energy, the balance setting up plant plankton and natural beneficial bacterium comes stabilizing water quality and water colour.
Containing yeast in biotechnological formulation raw material composition of the present invention, after yeast enters water body, abundant enzyme system can be produced, can dissolved organic matter be utilized fast as organic acid, sugar, peptide etc., make water quality pure and fresh, and the objectionable impuritiess such as Absorbable rod utilizes ammonia-state nitrogen, nitrite, can in sediment of pond anaerobic, the low dissolved oxygen of bottom, pond at the middle and upper levels excess oxygen can grow, and plays water purification, the effect of regulating water quality.
Containing water glass in biotechnological formulation raw material of the present invention composition, for pond waters provides soluble silicon salt, greatly can improve the reproduction speed of useful algae diatom, for it can form advantage algae kind in planktonic algae, and form excellent water colour and create favorable conditions.
The invention has the beneficial effects as follows: after biotechnological formulation of the present invention applies pond, after starting stirring, light blue is dissolved in water body uniformly, water colour shows light blue or blue, and water transparency is very fast becomes about 60-80cm from see the bottom (100-150cm), and the moss of such bottom and mud skin are due to by light blue shading, and lose light source, dead at ensuing 2 to 3 days, and the light blue color time length is long, about 10 to 20 days, and genus bacillus in biotechnological formulation now and yeast have activated in one to two day, start efficiently to produce amylase, residual bait in proteasome degradation pond, dead moss, mud skin, ight soil and other organic waste are converted into low molecular organic acids, nitrate, phosphoric acid salt, the inorganic salts such as vitriol, for the plant planktons such as algae provide nutrition, and the water glass in biotechnological formulation, for pond waters provides soluble silicon salt, greatly can improve the reproduction speed of useful algae diatom, accelerate pond material cycle and flow of energy, the balance setting up planktonic algae and probiotics carrys out stabilizing water quality, the excellent diatom water colour of final formation.
If the thorough clearly pond seen the bottom tongue not green for a long time, then after applying this biotechnological formulation, too due to the interception of light blue, genus bacillus and saccharomycetic decomposition bed mud, organism and promote algal grown, thus form real water colour, also can prevent the growth of moss.
Biotechnological formulation of the present invention serves control water body moss, decomposes organic, promotes the multifunctional biological agent that useful algae grows.The effect of the control moss of biotechnological formulation of the present invention far wins copper sulfate, potassium permanganate, prometryn etc., not only can be used for cultivating pool, also can be used for landscape water body, the control of the moss in the seawater of outbeach.Environmentally safe, toxicological harmless, biodegradable, low consumption, nuisanceless, simple to operate, the advantages such as purposes is wide.This technology tallies with the national condition and aquaculture technology developing direction, and in aquaculture, particularly Shrimp waste cultivation has broad application prospects.
The effect of biotechnological formulation of the present invention control moss is described below in conjunction with test.
One, this biotechnological formulation process mud skin
1, test site: sea-gull island, East, Fanyu, Guangzhou; Breed variety: Penaeus vannamei; Area: 12 mu; Culture style: Tu Tang raises together with; Put number of days in a suitable place to breed: 0 day;
2, problem describes: before putting seedling, water is thin, can see at the bottom of pond, has a large amount of mud skin at the bottom of pond;
3, case study: the pool having mud skin, be generally difficult to rich water, during fertilising, fertilizer absorbs all to mud skin, cause algae grow not, if just put seedling like this, can cause shrimp seedling stress, be lowered into motility rate;
4, solution: every mu uses this biotechnological formulation 100 grams;
5, result: after this biotechnological formulation uses, water colour is light blue, and transparency becomes 80cm from 120cm, the 3rd day, and a large amount of mud skins floats on the surface of the water.Within 3rd day and the 4th day, obviously can see that algae length has been got up, water colour is yellow-green colour;
6, sum up: this biotechnological formulation can reduce the transparency of water, and block sunlight directly shines at the bottom of pond.Mud skin, owing to lacking illumination, cannot carry out photosynthesis, dead floating.After death, the algae in the pool, because not had nutrient competition object, can grow mud skin fast.Simultaneously the genus bacillus of this biotechnological formulation and yeast can decompose mud skin, promote that useful algae grows, prevent water quality from degenerating.
Two, this biotechnological formulation process moss
1, test site: Leizhou City, Zhanjiang, Guangdong Province plant; Breed variety: Penaeus vannamei; Area: eight mu; Culture style: Tu Tang raises together with; Put number of days in a suitable place to breed: 30 days;
2, problem describes: in earlier stage, Changes in weather is fast in cultivation, algae, and water is thin, can see at the bottom of pond, has a large amount of moss at the bottom of several days after-bays;
3, case study: the pool having moss, be generally difficult to rich water, during fertilising, fertilizer absorbs all to mud skin, cause algae grow not, water is thin, and the end is poor, can cause shrimp stress, be lowered into motility rate;
4, solution: every mu uses this biotechnological formulation 100 grams;
5, result: after this biotechnological formulation uses, water colour is light blue, and transparency becomes 80cm from 110cm, the 3rd day, and a large amount of mosses is by green change lark, black.Within 3rd day and the 4th day, obviously can see that algae length has been got up, water colour is yellow-green colour;
6, sum up: this biotechnological formulation can reduce the transparency of water, and block sunlight directly shines at the bottom of pond.Moss, owing to lacking illumination, cannot carry out photosynthesis, dead.After death, the algae in the pool, because not had nutrient competition object, can grow moss fast.Simultaneously the genus bacillus of this biotechnological formulation and yeast can decompose moss, promote that useful algae grows.
Embodiment
Substantive distinguishing features of the present invention can be embodied from the following examples, but these embodiments are only as illustrating, instead of limits the invention.
Embodiment 1
The raw material preparing this biotechnological formulation is by weight: food grade light blue 50 parts, genus bacillus 30 parts, 10 parts, yeast, water glass 10 parts;
The preparation method of the biotechnological formulation of this control water body moss, comprises the steps:
(1), raw materials pretreatment: after 10 parts of water glass and the pre-mixing of 50 parts of food grade light blues, pulverized 100 orders;
(2), stirring and evenly mixing: add 30 parts of genus bacillus bacterium powder successively in stirred pot, 10 parts of yeast powder, pretreated light blue and water glass powder, open and stir 20-30 minute, can pack after mixing;
(3), double casing: after first being utilized by the biotechnological formulation mixed Soluble Plastic Packing to install by 100 grams/handbag, pack one deck with common plastics more outside, be finished product.
Embodiment 2
The raw material preparing this biotechnological formulation is by weight: food grade light blue 60 parts, genus bacillus 25 parts, 5 parts, yeast, water glass 10 parts;
The preparation method of the biotechnological formulation of this control water body moss, comprises the steps:
(1), raw materials pretreatment: after 10 parts of water glass and the pre-mixing of 60 parts of food grade light blues, pulverized 100 orders;
(2), stirring and evenly mixing: add 25 parts of genus bacillus bacterium powder successively in stirred pot, 5 parts of yeast powder, pretreated light blue and water glass powder, open and stir 20-30 minute, can pack after mixing;
(3), double casing: after first being utilized by the biotechnological formulation mixed Soluble Plastic Packing to install by 100 grams/handbag, pack one deck with common plastics more outside, be finished product.

Claims (6)

1. for preventing and treating a biotechnological formulation for water body moss, it is characterized in that, the raw material preparing this biotechnological formulation is by weight: food grade light blue 40-70 part, genus bacillus bacterium powder 20-40 part, yeast powder 5-15 part, water glass 5-15 part.
2. the preparation method of the biotechnological formulation for preventing and treating water body moss according to claim 1, is characterized in that, comprise the steps:
, raw materials pretreatment: after 5-15 part water glass and the pre-mixing of 40-70 part food grade light blue, pulverized 100 mesh sieves;
, stirring and evenly mixing: in stirred pot, add 20-40 part genus bacillus bacterium powder successively, 5-15 part yeast powder, step pretreated light blue and water glass powder, stir 20-30 minute, can pack after mixing;
, double casing: after first being utilized by the biotechnological formulation mixed Soluble Plastic Packing to install by 100 grams/handbag, pack one deck with common plastics more outside, be finished product.
3. biotechnological formulation for preventing and treating water body moss according to claim 1, it is characterized in that, described genus bacillus is subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus natto, bacillus amyloliquefaciens, bacillus megaterium, the mixture of one or more in bacillus pumilus.
4. biotechnological formulation for preventing and treating water body moss according to claim 1, is characterized in that, the spore content of genus bacillus bacterium powder is not less than 20,000,000,000 cfu/g, and it pulverized 100 mesh sieves.
5. biotechnological formulation for preventing and treating water body moss according to claim 1, is characterized in that, described yeast is yeast saccharomyces cerevisiae, Candida utilis, candida tropicalis, bread yeast, the mixture of one or more in cereuisiae fermentum.
6. biotechnological formulation for preventing and treating water body moss according to claim 1, is characterized in that, the viable bacteria content of yeast powder is not less than 2,000,000,000 cfu/g, and it pulverized 100 mesh sieves.
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