CN101082024A

CN101082024A - Preparation of holothurian pool bacterium and method for restoring sea cucumber cultivation pool environment

Info

Publication number: CN101082024A
Application number: CNA200610027130XA
Authority: CN
Inventors: 邹国忠
Original assignee: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co Ltd
Current assignee: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co Ltd
Priority date: 2006-05-31
Filing date: 2006-05-31
Publication date: 2007-12-05

Abstract

The sea cucumber pond bacterial preparation is environment friendly composite live bacterial preparation developed based on the characteristics of water and bottom material in sea cucumber raising pond and by means of modern biotechnology and for improving water quality and bottom material quality. The sea cucumber pond bacterial preparation has photosynthetic bacteria, bacillus, saccharomycetes and actinomycete as the main bacteria, and contains trace elements essential for culturing marine micro algae for raising sea cucumber. The microbes in the sea cucumber pond bacterial preparation is in dormant state for easy storing, and after being thrown into water, they are revived and disintegrated fast. They propagate to form dominant species to decompose organic pollutant in water, eliminate harmful matter, promote the growth of beneficial algae, inhibit the growth of harmful algae, and improve water and bottom material quality.

Description

Preparation of ginseng pond bacterium and the method for repairing the holothruian cultures pool environment

Bacterium is specially at holothurian cultivation pond water quality and substrate characteristics in the ginseng pond, use the compound viable bacteria water quality of environmental type, the substrate improved products of modern biotechnology exploitation, the photosynthetic bacterium that it goes out with the nature separation and Culture, genus bacillus, yeast, actinomycetes etc. are taken bacterial classification as the leading factor, simultaneously composite have cultivate the needed trace element of marine microalgae (diatom, green alga, chrysophyceae etc.) in the holothruian cultures process.Ginseng pond bacteria microorganism all is in dormant state, stablize and easily preservation, after rendering to water body, the dormancy viable bacteria can very fast recovery and disintegration, and breed into dominant population with multiple, decompose the organic pollutant in the water body rapidly, eliminate objectionable impuritiess such as amino nitrogen, nitroso-group nitrogen, hydrogen sulfide in the water, and be translated into the nutrition source of marine unicellular algae class, promote the growth of good marine algas such as diatom, green alga, chrysophyceae, suppress the breeding of harmful algae, play the effect of cultivating and improveing water quality, simultaneously can increase water transparency and dissolved oxygen, purify substrate.After useful bacterial strain is preponderated in water body, also can pass through nutrient competition, competition for space and secretion class antibiotic substance, suppress field planting and the invasion and attack of other pathogenic bacterium directly or indirectly, also can produce immunologic active material in addition, thereby reduce sickness rate.Ginseng pond bacterium is without any side effects to sea cucumber and other cultured fishes, shrimp, crab, shellfish, is that high-density breeding is cultivated the water body active bait, repairs the pond ecotope, improves the essential product of sea cucumber surviving rate.

[characteristics]

1, ginseng pond bacterium is adopted the dry concentration of vacuum and low temperature quick freezing, viable bacteria content height, long storage time effect stability.

2, ginseng pond bacterium need not activate, behind the input water body, and very fast recovery of dormancy viable bacteria and disintegration, and growth and breeding Cheng Shuizhong dominant microflora.

[physicochemical character] tawny powder.

[main component] photosynthetic bacterium, genus bacillus, yeast, actinomycetes, seawater algae trace element, nutrient salts etc.

[active constituent content] every gram contains 2 * 108 of probiotic bacteriums.

[use range] is applicable to all sea cucumber nursery ponds and growing pond (comprise and raise together with the pond), especially is fit to culture sea cucumber for many years, and mud is more, the aging pond that substrate is relatively poor.

[usage] evenly splashes with full pond, 10-20 times of Chi Shui dilution back.

[consumption]

1, improve water quality: every mu (1 meter of the depth of water) splashes once every 10-15 days with ginseng pond bacterium 100 grams.

2, improvement substrate: every mu (1 meter of the depth of water) splashes once every 10-15 days with ginseng pond bacterium 250 grams.

3, cultivate algae: every mu (1 meter of the depth of water) splashes according to water quality girth of a garment situation later at any time with ginseng pond bacterium 250-500 gram full pool spilling head, and water quality is crossed thin multiplicable splashing.

4, grow seedlings: seedling goes into pond first three day, and every cubic metre of water body 2 grams of splashing after seedling goes into the pond, are looked the water quality situation, splashes once every 2-3 days, and consumption is every cubic metre of water body 0.5-1 gram of splashing.

[attention]

1, throws in " ginseng pond bacterium " bans use of germicidal action in first three day, back five days pharmaceutical chemicals and antibiotic medicine.

Water temperature should reach more than 15 ℃ when 2, throwing in, and throws in back seven days and does not change water.

[summary of the invention]

Probiotics of the present invention and preparation method thereof, as follows:

1. fresh chicken manure is positioned over shady and cool place and carries out air-dryly, when treating that water content is 18-20%, collect standby.

2. 60 mesh sieves select rice bran, and are standby.

3. get EM bacterium bacterium liquid, standby.

4. get 1., 2., 3., brown sugar and water, by 45: 30: 2: 1: 22 mixed stirred.

5. will 4. pour the fermentation hole into, sealing and fermenting 10-15 days, shine dry grinding then, make fertilizer composite bacteria auxiliary material through the choosing of 80 mesh sieves, standby.

6. 80 mesh sieves select powdery saltpetre, lay-by material A; 80 mesh sieves select powdery sodium silicate, lay-by material B; 80 mesh sieves select the powdery ironic citrate, lay-by material C; 80 mesh sieves select powdery b diammonium edta sodium, lay-by material D.

7. get 5. fertilizer composite bacteria auxiliary material and 6. lay-by material A, B, C, D in 46: 30: 20: 2: 2 ratio is composite to mix.

The present invention has strong operability, with low cost, effect is remarkable, to the water surrounding non-secondary pollution, aquatic animal is had no side effect, culture environment of aquatic products there are advantages such as obvious repairing effect, be to comply with current social back to nature, treasure water and life, pursue green food, the development healthy aquaculture, the only way of realizing healthy consumption.

Claims

Bacterium is specially at holothurian cultivation pond water quality and substrate characteristics in the ginseng pond, use the compound viable bacteria water quality of environmental type, the substrate improved products of modern biotechnology exploitation, the photosynthetic bacterium that it goes out with the nature separation and Culture, genus bacillus, yeast, actinomycetes are taken bacterial classification as the leading factor, simultaneously composite have cultivate the needed trace element of marine microalgae (diatom, green alga, chrysophyceae) in the holothruian cultures process.Ginseng pond bacteria microorganism all is in dormant state, stablize and easily preservation, after rendering to water body, the dormancy viable bacteria can very fast recovery and disintegration, and breed into dominant population with multiple, decompose the organic pollutant in the water body rapidly, eliminate objectionable impuritiess such as amino nitrogen, nitroso-group nitrogen, hydrogen sulfide in the water, and be translated into the nutrition source of marine unicellular algae class, promote the growth of good marine algas such as diatom, green alga, chrysophyceae, suppress the breeding of harmful algae, play the effect of cultivating and improveing water quality, simultaneously can increase water transparency and dissolved oxygen, purify substrate.

[summary of the invention]

Probiotics of the present invention and preparation method thereof, as follows:

1. fresh chicken manure is positioned over shady and cool place and carries out air-dryly, when treating that water content is 18-20%, collect standby.

2. 60 mesh sieves select rice bran, and are standby.

3. get EM bacterium bacterium liquid, standby.

4. get 1., 2., 3., brown sugar and water, by 45: 30: 2: 1: 22 mixed stirred.

5. will 4. pour the fermentation hole into, sealing and fermenting 10-15 days, shine dry grinding then, make fertilizer composite bacteria auxiliary material through the choosing of 80 mesh sieves, standby.

6. 80 mesh sieves select powdery saltpetre, lay-by material A; 80 mesh sieves select powdery sodium silicate, lay-by material B; 80 mesh sieves select the powdery ironic citrate, lay-by material C; 80 mesh sieves select powdery b diammonium edta sodium, lay-by material D.

7. get 5. fertilizer composite bacteria auxiliary material and 6. lay-by material A, B, C, D in 46: 30: 20: 2: 2 ratio is composite to mix.