CN104232497A - EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation - Google Patents
EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation Download PDFInfo
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Abstract
The invention belongs to the field of microorganisms, and in particular relates to an EM flora preparation for improving water quality by compounding a plurality of strains and a preparation method of the EM flora preparation. The EM flora preparation contains rhodopseudomonas palustris, lactobacillus acidophilus, a wine brewing yeast, actinomycete aspergillus and bacillus subtilis, wherein the total bacterial concentration in the preparation is more than 30*10<8>cfu/ml, and the content of rhodopseudomonas palustris is more than or equal to 60%; the EM flora preparation is prepared from the strains by virtue of fermentation and dilution. The invention pointedly selects the EM flora preparation for improving water quality by compounding the plurality of strains specifically aiming at the environment of aquatic product breeding; by adopting the EM flora preparation, harmful amino nitrogen and nitroso nitrogen in water can be rapidly and completely degraded, and harmful substances such as hydrogen sulfide and the like can be removed; residual feeds and excrement in the water can be efficiently decomposed, water bottom sludge can be removed, and the water quality can be improved; and the number of probiotics in the water can be kept.
Description
Technical field
The invention belongs to microorganism field, particularly a kind of multi-cultur es compound EM flora preparation improving water quality and preparation method thereof.
Background technology
Along with society is for the raising of fishery products awareness, increasing fishery products become the delicious food in people's dish.Culture fishery is also increasingly flourishing.But due to being on the rise of nowadays environmental pollution, cultivation density and scale are also constantly soaring, propose more and more higher requirement to cultural technique.
EM bacterium, its English name is " Effective Microorganisms ", it is the active bacteria formulation being selected from more than 10 genus 80 multiple-microorganism compound criteria such as photosynthetic bacteria, actinomycetes, yeast, compound lactobacillus, genus bacillus, the mutual symbiosis of various microorganism, interaction in EM microbial inoculum, common development, give play to several functions, the organic material decomposition such as animals and plants residue can have been promoted rapidly, suppress the growth of spoilage organism and other germ.
The present invention is specially for the environment of aquaculture, and a kind of multi-cultur es compound of seed selection improves the EM flora preparation of water quality targetedly, can fast, thoroughly harmful amino nitrogen, nitroso-group nitrogen in degradation water, the objectionable impuritiess such as removal of hydrogen sulfide; Residual bait and movement in efficient-decomposition water, eliminate sludge underwater, improve water quality; Probiotic bacterium quantity in water can also be kept, suppress field planting and the invasion and attack of other pathogenic bacterium; Degradation of organic substances, active adsorption objectionable impurities, reduces water body acidity, stablizes water pH value, trace element supplement, improves water transparency, purifies water.
Summary of the invention
The technical problem to be solved in the present invention is: EM flora preparation providing a kind of multi-cultur es compound to improve water quality and preparation method thereof.
In a first aspect of the present invention, provide the EM flora preparation that a kind of multi-cultur es compound improves water quality, containing Rhodopseudomonas palustris, Lactobacterium acidophilum, yeast saccharomyces cerevisiae, unwrapping wire aspergillus, subtilis, the total bacteria concentration in preparation is greater than 30 × 10
8cfu/ml, wherein content>=60% of Rhodopseudomonas palustris.
In a second aspect of the present invention, provide the preparation method that a kind of multi-cultur es compound improves the EM flora preparation of water quality, it is characterized in that comprising following steps:
(1) preparation of nutrient solution
Ammonia chloride (NH
4cl): sodium bicarbonate (NaHCO
3): dipotassium hydrogen phosphate (K
2hPO
4): sodium-acetate (CH
3cOONa): magnesium chloride (MgCl
2): sodium-chlor (NaCl): glucose (C
6h
12o
6): distilled water (H
2o)=15:20:10:35:8:40:200:19672, fully dissolves, and makes nutrient solution for subsequent use;
(2) preparation of composite probiotics preparations
Get the Rhodopseudomonas palustris (Rhodopseudomonas palustris) in photosynthetic bacterium, bacterial classification content is 30 × 10
8cfu/ml or more; Lactobacterium acidophilum (Lactobacillus acidophilus) in milk-acid bacteria, bacterial classification content is 5 × 10
8cfu/ml or more; Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) in yeast, bacterial classification content is 20 × 10
8cfu/ml or more; Unwrapping wire aspergillus (Asporgillus cellulosae) bacterial classification content in filamentous fungus is 5 × 10
6cfu/ml or more; Subtilis (Bacillus subtilis), bacterial classification content is 60 × 10
8cfu/ml or more; Get the Rhodopseudomonas palustris of 8-12 part (volume), 4-6 part (volume) Lactobacterium acidophilum, 1-3 part (volume) brewer's yeast, 1-2 part (volume) unwrapping wire aspergillus, it is for subsequent use that 1-3 part (volume) subtilis obtains bacterial classification;
(3) preparation of composite bacteria mother liquor
The ratio of getting step (2) gained bacterial classification and step (1) gained nutrient solution 1:10-20 is by volume poured in fermentor tank, and keep temperature 25-30 DEG C, anaerobically fermenting cultivates 4-10 days, obtained composite bacteria mother liquor;
(4) getting the composite bacteria mother liquor that step (3) obtains carries out composite with 40-60 times of deionized water, and obtained composite bacteria diluent, obtains the EM flora preparation that multi-cultur es compound improves water quality.
Because the fermentation of EM bacterium is due to the difference of season and fermentation time, the product that color is different is obtained, in order to make product have homogeneous color and luster and outward appearance, the dyestuff harmless to biology can also be used to dye to the composite bacteria diluent obtained in step (4).
Except indicating especially, the number in the present invention refers to mass fraction.
Beneficial effect of the present invention: the efficacious microbial colony preparation that multi-cultur es compound of the present invention improves aquaculture water water quality is by screening efficient water purification viable bacteria bacterial classification.This product liquid preparation, Viable detection is high, directly splashes, successful, uses very convenient; And amino nitrogen, nitroso-group nitrogen can be harmful to, the objectionable impuritiess such as removal of hydrogen sulfide in quick, thorough degradation water; Residual bait and movement in efficient-decomposition water, eliminate sludge underwater, improve water quality; Probiotic bacterium quantity in water can also be kept, suppress field planting and the invasion and attack of other pathogenic bacterium; Degradation of organic substances, active adsorption objectionable impurities, reduces water body acidity, stablizes water pH value, trace element supplement, improves water transparency, purifies water.
The efficacious microbial colony preparation that compound of the present invention improves aquaculture water water quality has following effect:
1, Abwehrkraft des Koepers is promoted: microorganism species preparation of the present invention has the mobility of good promotion aquaculture product, improves the ability of its resist the disease;
2, substrate is improved: residual bait, ight soil, animals and plants corpse and organic debris at the bottom of degraded pond;
3, purify water: the objectionable impuritiess such as ammonia nitrogen, nitrite, hydrogen sulfide, Algae toxins at the bottom of active adsorption pond;
4, keep probiotic bacterium and planktonic organism proportional balancing method in water, suppress the flourish of harmful algae;
5, water transparency is improved.
The efficacious microbial colony preparation that compound of the present invention improves aquaculture water water quality has following features:
1, this product liquid preparation, the survival rate of thalline is high, easy to use.
2, this product can be preserved at normal temperatures, avoids troublesome preserving type.
3, this product strong adaptability, has stronger tolerance to chemical disinfection medicine, insecticidal materials and antibiotic etc.
Embodiment
Embodiment 1
(1) preparation of nutrient solution
Ammonia chloride (NH
4cl): sodium bicarbonate (NaHCO
3): dipotassium hydrogen phosphate (K
2hPO
4): sodium-acetate (CH
3cOONa): magnesium chloride (MgCl
2): sodium-chlor (NaCl): glucose (C
6h
12o
6): distilled water (H
2o)=15:20:10:35:8:40:200:19672, fully dissolves, and makes nutrient solution for subsequent use;
(2) preparation of composite probiotics preparations
Get the Rhodopseudomonas palustris (Rhodopseudomonas palustris) in photosynthetic bacterium, bacterial classification content is 30 × 10
8cfu/ml; Lactobacterium acidophilum (Lactobacillus acidophilus) in milk-acid bacteria, bacterial classification content is 5 × 10
8cfu/ml; Brewer's yeast (Saccharomyces cerevisiae) in yeast, bacterial classification content is 20 × 10
8cfu/ml; Unwrapping wire aspergillus (Asporgillus cellulosae) bacterial classification content in filamentous fungus is 5 × 10
6cfu/ml; Subtilis (Bacillus subtilis), bacterial classification content is 60 × 10
8cfu/ml; Get the Rhodopseudomonas palustris of 12 parts of volumes, the Lactobacterium acidophilum of 5 parts of volumes, the brewer's yeast of 2 parts of volumes, the unwrapping wire aspergillus of 1 part of volume, it is for subsequent use that the subtilis of 2 parts of volumes obtains bacterial classification;
(3) preparation of composite bacteria mother liquor
The ratio of getting step (2) gained bacterial classification and step (1) gained nutrient solution 1:19 is by volume poured in fermentor tank, and keep temperature 25-30 DEG C, anaerobically fermenting cultivates 6 days, obtained composite bacteria mother liquor;
(4) getting the composite bacteria mother liquor that step (3) obtains carries out composite with 49 times of deionized waters, obtained composite bacteria diluent, get gardenia blue pigment and composite bacteria diluent mixes according to the ratio of mass ratio 1:1000 is composite, make blue composite bacteria diluent and namely obtain the EM flora preparation that multi-cultur es compound improves water quality.
Embodiment 2
Process water body: the Penaeus vannamei pool of two adjacent 25 mu of waters surface, the depth of water is about 1 meter, the shrimp seedling release time is April 28, injected volume is 40,000 tails/mu, be divided into experimental group and control group, treatment cycle is 2 months, within first 20 days every 10 days, uses 1 time, the EM flora preparation 200ml that every mu of water surface embodiment 1 obtains; Latter 40 days every 8 days with 1 time, every mu of water surface this product 450ml.Experimental session control group and experimental group to be thrown something and fed feed twice equal every day, and the morning, 9:00 threw something and fed once, and 17:00 throws something and feeds once at dusk, and other rearing conditions are all identical.Within 20 days after putting seedling, rise, survey control group and experimental group pond water quality index, measured once every 5 days, tie-in 6 times, averaged (seeing the following form):
Claims (3)
1. multi-cultur es compound improves an EM flora preparation for water quality, it is characterized in that, containing Rhodopseudomonas palustris, Lactobacterium acidophilum, yeast saccharomyces cerevisiae, unwrapping wire aspergillus, subtilis, the total bacteria concentration in preparation is greater than 30 × 10
8cfu/ml, wherein content>=60% of Rhodopseudomonas palustris.
2. prepare a method for EM flora preparation as claimed in claim 1, it is characterized in that comprising following steps:
(1) preparation of nutrient solution
Ammonia chloride (NH
4cl): sodium bicarbonate (NaHCO
3): dipotassium hydrogen phosphate (K
2hPO
4): sodium-acetate (CH
3cOONa): magnesium chloride (MgCl
2): sodium-chlor (NaCl): glucose (C
6h
12o
6): distilled water (H
2o)=15:20:10:35:8:40:200:19672, fully dissolves, and makes nutrient solution for subsequent use;
(2) preparation of composite probiotics preparations
Get the Rhodopseudomonas palustris in photosynthetic bacterium, bacterial classification content is 30 × 10
8cfu/ml or more; Lactobacterium acidophilum in milk-acid bacteria, bacterial classification content is 5 × 10
8cfu/ml or more; Yeast saccharomyces cerevisiae in yeast, bacterial classification content is 20 × 10
8cfu/ml or more; Unwrapping wire aspergillus bacterial classification content in filamentous fungus is 5 × 10
6cfu/ml or more; Subtilis, bacterial classification content is 60 × 10
8cfu/ml or more; Get the Rhodopseudomonas palustris of 8-12 part, 4-6 part Lactobacterium acidophilum, 1-3 part brewer's yeast, 1-2 part unwrapping wire aspergillus, it is for subsequent use that 1-3 part subtilis obtains bacterial classification;
(3) preparation of composite bacteria mother liquor
The ratio of getting step (2) gained bacterial classification and step (1) gained nutrient solution 1:10-20 is by volume poured in fermentor tank, and keep temperature 25-30 DEG C, anaerobically fermenting cultivates 4-10 days, obtained composite bacteria mother liquor;
(4) getting the composite bacteria mother liquor that step (3) obtains carries out composite with 40-60 times of deionized water, and obtained composite bacteria diluent, obtains the EM flora preparation that multi-cultur es compound improves water quality.
3. prepare as claimed in claim 2 the method for EM flora preparation, characterized by further comprising and get gardenia blue pigment and composite bacteria diluent according to the composite step mixed of the ratio of mass ratio 1:1000.
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| CN104745487A (en) * | 2015-03-25 | 2015-07-01 | 广东海纳川药业股份有限公司 | Complex microbial inoculant for water quality purification and application thereof |
| CN107364975A (en) * | 2017-07-10 | 2017-11-21 | 芜湖市木子生态园有限公司 | A kind of Aquacultural water modifying agent |
| CN107535393A (en) * | 2017-09-08 | 2018-01-05 | 无为县慧富水蛭养殖专业合作社 | A kind of degraded dispensing of hirudiniculture |
| CN108094283A (en) * | 2017-12-29 | 2018-06-01 | 海南中正水产科技有限公司 | A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling |
| CN108587966A (en) * | 2018-05-07 | 2018-09-28 | 江苏大有生物科技发展有限公司 | A kind of application of pure plant source EM bacterium culture mediums and its fermented product in aquaculture |
| CN109136121A (en) * | 2017-06-15 | 2019-01-04 | 财团法人食品工业发展研究所 | Lactobacillus, method for preparing pigment using the same, lactobacillus culture and pigment composition comprising the same |
| CN109430579A (en) * | 2018-12-10 | 2019-03-08 | 上海泓宝绿色水产股份有限公司 | EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method |
| CN109607818A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of microorganism improver of water quality and preparation method thereof |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN112280711A (en) * | 2020-11-02 | 2021-01-29 | 无锡泓宝微生物科技有限公司 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
| CN112812984A (en) * | 2019-11-18 | 2021-05-18 | 无锡泓宝微生物科技有限公司 | Preparation method and application method of compound microbial preparation for comprehensive planting and breeding of rice and fish |
| CN112850913A (en) * | 2020-12-31 | 2021-05-28 | 上海泓宝绿色水产股份有限公司 | Bdellodicus bdellovi and preparation method and use method thereof |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745487A (en) * | 2015-03-25 | 2015-07-01 | 广东海纳川药业股份有限公司 | Complex microbial inoculant for water quality purification and application thereof |
| CN109136121A (en) * | 2017-06-15 | 2019-01-04 | 财团法人食品工业发展研究所 | Lactobacillus, method for preparing pigment using the same, lactobacillus culture and pigment composition comprising the same |
| CN107364975A (en) * | 2017-07-10 | 2017-11-21 | 芜湖市木子生态园有限公司 | A kind of Aquacultural water modifying agent |
| CN107535393A (en) * | 2017-09-08 | 2018-01-05 | 无为县慧富水蛭养殖专业合作社 | A kind of degraded dispensing of hirudiniculture |
| CN108094283A (en) * | 2017-12-29 | 2018-06-01 | 海南中正水产科技有限公司 | A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling |
| CN108587966B (en) * | 2018-05-07 | 2020-09-22 | 江苏大有生物科技发展有限公司 | Pure natural plant source EM (effective microorganisms) culture medium and application of fermentation product thereof in aquaculture |
| CN108587966A (en) * | 2018-05-07 | 2018-09-28 | 江苏大有生物科技发展有限公司 | A kind of application of pure plant source EM bacterium culture mediums and its fermented product in aquaculture |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN109430579A (en) * | 2018-12-10 | 2019-03-08 | 上海泓宝绿色水产股份有限公司 | EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method |
| CN109607818A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of microorganism improver of water quality and preparation method thereof |
| CN112812984A (en) * | 2019-11-18 | 2021-05-18 | 无锡泓宝微生物科技有限公司 | Preparation method and application method of compound microbial preparation for comprehensive planting and breeding of rice and fish |
| CN112280711A (en) * | 2020-11-02 | 2021-01-29 | 无锡泓宝微生物科技有限公司 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
| CN112850913A (en) * | 2020-12-31 | 2021-05-28 | 上海泓宝绿色水产股份有限公司 | Bdellodicus bdellovi and preparation method and use method thereof |
| CN112850913B (en) * | 2020-12-31 | 2023-04-18 | 上海泓宝绿色水产股份有限公司 | Bdellodicus bdellovi and preparation method and use method thereof |
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