CN110713944A - Deodorizing microbial preparation for breeding house and manufacturing process - Google Patents
Deodorizing microbial preparation for breeding house and manufacturing process Download PDFInfo
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- CN110713944A CN110713944A CN201810766504.2A CN201810766504A CN110713944A CN 110713944 A CN110713944 A CN 110713944A CN 201810766504 A CN201810766504 A CN 201810766504A CN 110713944 A CN110713944 A CN 110713944A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 59
- 238000009395 breeding Methods 0.000 title claims abstract description 24
- 230000001488 breeding effect Effects 0.000 title claims abstract description 24
- 230000000813 microbial effect Effects 0.000 title claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 14
- 230000001877 deodorizing effect Effects 0.000 title claims description 18
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 70
- 241000894006 Bacteria Species 0.000 claims abstract description 64
- 239000001963 growth medium Substances 0.000 claims abstract description 43
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 35
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 35
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 35
- 241000194031 Enterococcus faecium Species 0.000 claims abstract description 35
- 239000004310 lactic acid Substances 0.000 claims abstract description 35
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 35
- 239000007787 solid Substances 0.000 claims abstract description 35
- 241001446247 uncultured actinomycete Species 0.000 claims abstract description 35
- 241001509286 Thiobacillus denitrificans Species 0.000 claims abstract description 29
- 238000002156 mixing Methods 0.000 claims abstract description 27
- 238000007789 sealing Methods 0.000 claims abstract description 18
- 238000012258 culturing Methods 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims description 36
- 230000001954 sterilising effect Effects 0.000 claims description 36
- 238000004659 sterilization and disinfection Methods 0.000 claims description 36
- 230000001580 bacterial effect Effects 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 27
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 26
- 230000008569 process Effects 0.000 claims description 14
- 235000010265 sodium sulphite Nutrition 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 230000003321 amplification Effects 0.000 claims description 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 238000007689 inspection Methods 0.000 claims description 6
- 239000008101 lactose Substances 0.000 claims description 6
- 230000008520 organization Effects 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 239000008223 sterile water Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 4
- 238000007711 solidification Methods 0.000 claims description 4
- 230000008023 solidification Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 18
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 6
- 229910021529 ammonia Inorganic materials 0.000 abstract description 3
- 238000004332 deodorization Methods 0.000 abstract description 3
- 229910017464 nitrogen compound Inorganic materials 0.000 abstract description 3
- 150000002830 nitrogen compounds Chemical class 0.000 abstract description 3
- 241000228212 Aspergillus Species 0.000 abstract description 2
- 239000002781 deodorant agent Substances 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 12
- 241000605118 Thiobacillus Species 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
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Abstract
The invention discloses a deodorant microbial preparation for breeding houses and a preparation process thereof, wherein the preparation comprises 12-27% of solid lactic acid bacteria, 8-13% of bacillus subtilis, 7-12% of thiobacillus denitrificans, 13-28% of candida utilis, 14-19% of enterococcus faecium and 16-21% of actinomycete; the manufacturing process comprises the following steps: step one, selecting strains; step two, preparing a culture medium; step three, culturing strains; step four, mixing strains; step five, preparing a preparation artificially; and step six, sealing and storing. According to the invention, the preparation prepared from solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and aspergillus actinomycete is selected, the preparation method is simple and easy to operate, the deodorization effect is good through multi-strain mixed preparation, nitrogen compounds can be decomposed, the ammonia odor of the breeding house is effectively reduced, and the odor environment of the breeding house is effectively improved.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a deodorizing microbial preparation for a breeding house and a preparation process thereof.
Background
In the process of cultivation, stink pollution is an important factor influencing the sanitary conditions of a cultivation house, a large amount of sewage and excrement can be accumulated in the cultivation house, so that the stink pollution in the cultivation house is serious, if the cultivation house is not deodorized, a large amount of bacteria can be bred due to long-term fermentation of the sewage and excrement, the cultivation safety of cultivation types is seriously influenced, and large economic loss is caused.
Disclosure of Invention
The invention aims to provide a culture house deodorizing microbial preparation and a manufacturing process thereof, which aim to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a deodorizing microbial preparation for breeding houses comprises 12-27% of solid lactic acid bacteria, 8-13% of bacillus subtilis, 7-12% of thiobacillus denitrificans, 13-28% of candida utilis, 14-19% of enterococcus faecium and 16-21% of actinomycete.
A manufacturing process of a deodorizing microbial preparation for a breeding house comprises the following steps: step one, selecting strains; step two, preparing a culture medium; step three, culturing strains; step four, mixing strains; step five, preparing a preparation artificially; step six, sealing and storing;
in the first step, purchasing solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete bacteria from a professional bacteria organization, screening the bacteria, removing deteriorated and damaged bacteria, and ensuring that the selected bacteria are proper in age, pure and healthy and have no diseases;
in the second step, peptone, beef extract, yeast extract and agar are added into water, heated and dissolved, and then K is added2PO4Adding water after dissolution, adjusting the pH value to be alkalescent, then adding lactose, uniformly mixing and dissolving, sealing by using a sealing film, sleeving a rubber band, putting into a sterilization pot for damp-heat sterilization, opening the sterilization pot to take out the culture medium when the pressure of a pressure instrument is reduced to zero after the sterilization is finished, weighing sodium sulfite into a sterile empty test tube, dissolving the sodium sulfite by using a small amount of sterile water, boiling in a water bath, immediately dropwise adding into an alkaline re-reddened ethanol solution, completely adding the mixed solution into the sterilized culture medium which is still in a molten state, uniformly mixing, immediately pouring a flat plate, and refrigerating for later use after solidification;
in the third step, the culture medium obtained in the second step is uniformly divided into seven parts, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete selected in the first step are independently added into the culture medium, amplification culture is carried out by a scribing method, the colony growth condition on the culture medium is observed, a single colony which is well dispersed is selected for standby, and the single colony is prepared and stored according to the proportion of 12-27% of the solid lactic acid bacteria, 8-13% of the bacillus subtilis, 7-12% of the thiobacillus denitrificans, 13-28% of the candida utilis, 14-19% of the enterococcus faecium and 16-21% of the actinomycete;
in the fourth step, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete obtained in the third step are put into a liquid stirrer to be stirred to obtain mixed bacterial colonies, and the bacterial colonies are uniformly stirred in the stirring process without obvious blocks;
in the fifth step, the mixed bacterial colony obtained in the fourth step is placed in a low-temperature environment for low-temperature drying, the water content after drying is 6%, and the preparation is obtained after drying;
in the sixth step, the preparation obtained in the fifth step is packaged, the appearance of the packaging bag is observed to be complete, and the preparation qualified in quality inspection is stored at low temperature.
According to the technical scheme, in the second step, the temperature of the damp-heat sterilization is 115 ℃, and the sterilization time is 20 min.
According to the technical scheme, in the fourth step, the rotating speed of the liquid stirrer is 20-30 r/min.
According to the technical scheme, in the fifth step, the low-temperature drying temperature is 4 ℃.
According to the technical scheme, in the sixth step, the storage temperature of the preparation is 4-10 ℃.
Compared with the prior art, the invention has the beneficial effects that:
1. the preparation prepared from solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and aspergillus actinomycete is selected, and the preparation has good deodorization effect through multi-strain mixing, can decompose nitrogen compounds, effectively relieves the ammonia odor of a breeding house, and effectively improves the odor environment of the breeding house;
2. the method comprises the steps of independently adding selected solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete into a culture medium, carrying out amplification culture by a scribing method, observing the growth condition of bacterial colonies on the culture medium, selecting single bacterial colonies with good dispersion for later use, mixing the bacterial colonies, stirring, and drying at low temperature to prepare the deodorant microbial preparation.
Drawings
FIG. 1 is a flow chart of the fabrication process of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution: a culture house deodorization microbial preparation and a preparation process thereof are as follows:
example 1:
a microbial preparation for deodorizing breeding houses comprises 20% of solid lactic acid bacteria, 11% of bacillus subtilis, 10% of thiobacillus denitrificans, 24% of candida utilis, 16% of enterococcus faecium and 19% of actinomycete.
A manufacturing process of a deodorizing microbial preparation for a breeding house comprises the following steps: step one, selecting strains; step two, preparing a culture medium; step three, culturing strains; step four, mixing strains; step five, preparing a preparation artificially; step six, sealing and storing;
in the first step, purchasing solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete bacteria from a professional bacteria organization, screening the bacteria, removing deteriorated and damaged bacteria, and ensuring that the selected bacteria are proper in age, pure and healthy and have no diseases;
in the second step, peptone, beef extract, yeast extract and agar are added into water, heated and dissolved, and then K is added2PO4Adding water after dissolution, adjusting pH value to alkalescence, adding lactose, mixing uniformly, sealing with sealing film, covering with rubber band, placing into a sterilization pot for wet heat sterilization, opening the sterilization pot to take out the culture medium when the pressure of the pressure instrument is reduced to zero after sterilization is completed, and weighing sodium sulfite until the culture medium is sterileDissolving in a small amount of sterile water in an empty test tube, boiling in water bath, immediately dripping in alkaline ethanol solution, adding the mixed solution into the sterilized culture medium, mixing, immediately pouring into a flat plate, solidifying, and refrigerating;
in the third step, the culture medium obtained in the second step is uniformly divided into seven parts, the solid lactic acid bacteria, the bacillus subtilis, the denitrified thiobacillus, the candida utilis, the enterococcus faecium and the actinomycete selected in the first step are independently added into the culture medium, amplification culture is carried out by a scribing method, the colony growth condition on the culture medium is observed, a single colony with better dispersion is selected for standby, and the single colony is prepared according to the proportion of 20% of solid lactic acid bacteria, 11% of bacillus subtilis, 10% of denitrified thiobacillus, 24% of candida utilis, 16% of enterococcus faecium and 19% of actinomycete and stored;
in the fourth step, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete obtained in the third step are put into a liquid stirrer to be stirred to obtain mixed bacterial colonies, and the bacterial colonies are uniformly stirred in the stirring process without obvious blocks;
in the fifth step, the mixed bacterial colony obtained in the fourth step is placed in a low-temperature environment for low-temperature drying, the water content after drying is 6%, and the preparation is obtained after drying;
in the sixth step, the preparation obtained in the fifth step is packaged, the appearance of the packaging bag is observed to be complete, and the preparation qualified in quality inspection is stored at low temperature.
In the second step, the temperature of the moist heat sterilization is 115 ℃, the sterilization time is 20min, the bacteria in the culture medium can be thoroughly killed, and the bacteria are prevented from polluting the subsequent enlarged culture of bacterial colonies; in the fourth step, the rotating speed of the liquid stirrer is 20-30r/min, which is beneficial to improving the mixing effect of bacterial colonies and enabling the mixing to be more uniform; in the fifth step, the low-temperature drying temperature is 4 ℃, and the drying effect can be improved by low-temperature drying, so that the inactivation of microorganisms caused by high-temperature drying is avoided; in the sixth step, the preservation temperature of the preparation is 4-10 ℃, the activity of microorganisms can be improved, and the loss of activity of microorganisms due to improper temperature in life can be avoided.
Example 2:
a deodorizing microbial preparation for breeding houses comprises solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete, wherein the solid lactic acid bacteria, the bacillus subtilis 12, the thiobacillus denitrificans 11, the candida utilis 20, the enterococcus faecium 14 and the actinomycete 18 are mixed in proportion.
A manufacturing process of a deodorizing microbial preparation for a breeding house comprises the following steps: step one, selecting strains; step two, preparing a culture medium; step three, culturing strains; step four, mixing strains; step five, preparing a preparation artificially; step six, sealing and storing;
in the first step, purchasing solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete bacteria from a professional bacteria organization, screening the bacteria, removing deteriorated and damaged bacteria, and ensuring that the selected bacteria are proper in age, pure and healthy and have no diseases;
in the second step, peptone, beef extract, yeast extract and agar are added into water, heated and dissolved, and then K is added2PO4Adding water after dissolution, adjusting the pH value to be alkalescent, then adding lactose, uniformly mixing and dissolving, sealing by using a sealing film, sleeving a rubber band, putting into a sterilization pot for damp-heat sterilization, opening the sterilization pot to take out the culture medium when the pressure of a pressure instrument is reduced to zero after the sterilization is finished, weighing sodium sulfite into a sterile empty test tube, dissolving the sodium sulfite by using a small amount of sterile water, boiling in a water bath, immediately dropwise adding into an alkaline re-reddened ethanol solution, completely adding the mixed solution into the sterilized culture medium which is still in a molten state, uniformly mixing, immediately pouring a flat plate, and refrigerating for later use after solidification;
in the third step, the culture medium obtained in the second step is uniformly divided into seven parts, the solid lactic acid bacteria, the bacillus subtilis, the denitrified thiobacillus, the candida utilis, the enterococcus faecium and the actinomycete selected in the first step are independently added into the culture medium, amplification culture is carried out by a scribing method, the colony growth condition on the culture medium is observed, a single colony with better dispersion is selected for standby, and the single colony is prepared according to the proportion of 25 percent of solid lactic acid bacteria, 12 percent of bacillus subtilis, 11 percent of denitrified thiobacillus, 20 percent of candida utilis, 14 percent of enterococcus faecium and 18 percent of actinomycete and stored;
in the fourth step, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete obtained in the third step are put into a liquid stirrer to be stirred to obtain mixed bacterial colonies, and the bacterial colonies are uniformly stirred in the stirring process without obvious blocks;
in the fifth step, the mixed bacterial colony obtained in the fourth step is placed in a low-temperature environment for low-temperature drying, the water content after drying is 6%, and the preparation is obtained after drying;
in the sixth step, the preparation obtained in the fifth step is packaged, the appearance of the packaging bag is observed to be complete, and the preparation qualified in quality inspection is stored at low temperature.
In the second step, the temperature of the moist heat sterilization is 115 ℃, the sterilization time is 20min, the bacteria in the culture medium can be thoroughly killed, and the bacteria are prevented from polluting the subsequent enlarged culture of bacterial colonies; in the fourth step, the rotating speed of the liquid stirrer is 20-30r/min, which is beneficial to improving the mixing effect of bacterial colonies and enabling the mixing to be more uniform; in the fifth step, the low-temperature drying temperature is 4 ℃, and the drying effect can be improved by low-temperature drying, so that the inactivation of microorganisms caused by high-temperature drying is avoided; in the sixth step, the preservation temperature of the preparation is 4-10 ℃, the activity of microorganisms can be improved, and the loss of activity of microorganisms due to improper temperature in life can be avoided.
Example 3:
a microbial preparation for deodorizing breeding houses comprises solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete, wherein the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete are 15%, 9%, 8%, 28%, 19% and 21% respectively.
A manufacturing process of a deodorizing microbial preparation for a breeding house comprises the following steps: step one, selecting strains; step two, preparing a culture medium; step three, culturing strains; step four, mixing strains; step five, preparing a preparation artificially; step six, sealing and storing;
in the first step, purchasing solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete bacteria from a professional bacteria organization, screening the bacteria, removing deteriorated and damaged bacteria, and ensuring that the selected bacteria are proper in age, pure and healthy and have no diseases;
in the second step, peptone, beef extract, yeast extract and agar are added into water, heated and dissolved, and then K is added2PO4Adding water after dissolution, adjusting the pH value to be alkalescent, then adding lactose, uniformly mixing and dissolving, sealing by using a sealing film, sleeving a rubber band, putting into a sterilization pot for damp-heat sterilization, opening the sterilization pot to take out the culture medium when the pressure of a pressure instrument is reduced to zero after the sterilization is finished, weighing sodium sulfite into a sterile empty test tube, dissolving the sodium sulfite by using a small amount of sterile water, boiling in a water bath, immediately dropwise adding into an alkaline re-reddened ethanol solution, completely adding the mixed solution into the sterilized culture medium which is still in a molten state, uniformly mixing, immediately pouring a flat plate, and refrigerating for later use after solidification;
in the third step, the culture medium obtained in the second step is uniformly divided into seven parts, the solid lactic acid bacteria, the bacillus subtilis, the denitrified thiobacillus, the candida utilis, the enterococcus faecium and the actinomycete selected in the first step are independently added into the culture medium, amplification culture is carried out by a scribing method, the colony growth condition on the culture medium is observed, a single colony with better dispersion is selected for standby, and the single colony is prepared according to the proportion of 15 percent of solid lactic acid bacteria, 9 percent of bacillus subtilis, 8 percent of denitrified thiobacillus, 28 percent of candida utilis, 19 percent of enterococcus faecium and 21 percent of actinomycete and stored;
in the fourth step, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete obtained in the third step are put into a liquid stirrer to be stirred to obtain mixed bacterial colonies, and the bacterial colonies are uniformly stirred in the stirring process without obvious blocks;
in the fifth step, the mixed bacterial colony obtained in the fourth step is placed in a low-temperature environment for low-temperature drying, the water content after drying is 6%, and the preparation is obtained after drying;
in the sixth step, the preparation obtained in the fifth step is packaged, the appearance of the packaging bag is observed to be complete, and the preparation qualified in quality inspection is stored at low temperature.
In the second step, the temperature of the moist heat sterilization is 115 ℃, the sterilization time is 20min, the bacteria in the culture medium can be thoroughly killed, and the bacteria are prevented from polluting the subsequent enlarged culture of bacterial colonies; in the fourth step, the rotating speed of the liquid stirrer is 20-30r/min, which is beneficial to improving the mixing effect of bacterial colonies and enabling the mixing to be more uniform; in the fifth step, the low-temperature drying temperature is 4 ℃, and the drying effect can be improved by low-temperature drying, so that the inactivation of microorganisms caused by high-temperature drying is avoided; in the sixth step, the preservation temperature of the preparation is 4-10 ℃, the activity of microorganisms can be improved, and the loss of activity of microorganisms due to improper temperature in life can be avoided.
Based on the above, the method has the advantages that the method purchases solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete strains from a professional strain organization, screens the strains, removes deteriorated and damaged strains, ensures that the selected strains are suitable for age, pure and healthy, and has no diseases; adding peptone, beef extract, yeast extract and agar into water, heating for dissolving, and adding K2PO4Adding water after dissolving, adjusting pH to alkalescence, adding lactose, mixing, sealing with sealing film, covering with rubber band, and placing into a containerCarrying out damp-heat sterilization in a sterilization pot, wherein the temperature of the damp-heat sterilization is 115 ℃, the sterilization time is 20min, bacteria in a culture medium can be thoroughly killed, the bacteria are prevented from polluting the subsequent enlarged culture of bacterial colonies, after the sterilization is finished, the sterilization pot is opened to take out the culture medium when the pressure of a pressure instrument is reduced to zero, then sodium sulfite is weighed into a sterile empty test tube, a small amount of sterile water is used for dissolving the sodium sulfite, the sodium sulfite is boiled in a water bath, the sodium sulfite is immediately dripped into an alkaline re-reddening ethanol solution, the mixed solution is completely added into the sterilized culture medium which still keeps a melting state, the mixed solution is immediately poured into a flat plate after being uniformly mixed, and the mixed solution is refrigerated for standby after being solidified; uniformly dividing the obtained culture medium into seven parts, independently adding the selected solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete into the culture medium, carrying out amplification culture by a scribing method, observing the growth condition of bacterial colonies on the culture medium, selecting well-dispersed single bacterial colonies for later use, preparing according to the proportion of 15% of solid lactic acid bacteria, 9% of bacillus subtilis, 8% of thiobacillus denitrificans, 28% of candida utilis, 19% of enterococcus faecium and 21% of actinomycete, and storing; the obtained solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete are put into a liquid stirrer for stirring, the rotating speed of the liquid stirrer is 20-30r/min, the mixing effect of bacterial colonies is favorably improved, the mixing is more uniform, mixed bacterial colonies are obtained, and the bacterial colonies are uniformly stirred in the stirring process without obvious blocks; placing the obtained mixed bacterial colony in a low-temperature environment for low-temperature drying at the temperature of 4 ℃, wherein the drying effect can be improved by low-temperature drying, the inactivation of microorganisms caused by high-temperature drying is avoided, the water content after drying is 6%, and the preparation is obtained after drying; the preparation is bagged, the appearance of the packaging bag is observed to be complete, the preparation qualified by quality inspection is stored at low temperature, and the activity of microorganisms can be improvedThe odor effect is good, nitrogen compounds can be decomposed, the ammonia odor of the breeding house is effectively reduced, and the odor environment of the breeding house is effectively improved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A deodorizing microbial preparation for breeding houses comprises 12-27% of solid lactic acid bacteria, 8-13% of bacillus subtilis, 7-12% of thiobacillus denitrificans, 13-28% of candida utilis, 14-19% of enterococcus faecium and 16-21% of actinomycete.
2. A manufacturing process of a deodorizing microbial preparation for a breeding house comprises the following steps: step one, selecting strains; step two, preparing a culture medium; step three, culturing strains; step four, mixing strains; step five, preparing a preparation artificially; step six, sealing and storing; the method is characterized in that:
in the first step, purchasing solid lactic acid bacteria, bacillus subtilis, thiobacillus denitrificans, candida utilis, enterococcus faecium and actinomycete bacteria from a professional bacteria organization, screening the bacteria, removing deteriorated and damaged bacteria, and ensuring that the selected bacteria are proper in age, pure and healthy and have no diseases;
in the second step, peptone, beef extract, yeast extract and agar are added into water, heated and dissolved, and then K is added2PO4Adding water after dissolution, adjusting the pH value to be alkalescent, then adding lactose, uniformly mixing and dissolving, sealing by using a sealing film, sleeving a rubber band, putting into a sterilization pot for damp-heat sterilization, opening the sterilization pot to take out the culture medium when the pressure of a pressure instrument is reduced to zero after the sterilization is finished, weighing sodium sulfite into a sterile empty test tube, dissolving the sodium sulfite by using a small amount of sterile water, boiling in a water bath, immediately dropwise adding into an alkaline re-reddened ethanol solution, completely adding the mixed solution into the sterilized culture medium which is still in a molten state, uniformly mixing, immediately pouring a flat plate, and refrigerating for later use after solidification;
in the third step, the culture medium obtained in the second step is uniformly divided into seven parts, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete selected in the first step are independently added into the culture medium, amplification culture is carried out by a scribing method, the colony growth condition on the culture medium is observed, a single colony which is well dispersed is selected for standby, and the single colony is prepared and stored according to the proportion of 12-27% of the solid lactic acid bacteria, 8-13% of the bacillus subtilis, 7-12% of the thiobacillus denitrificans, 13-28% of the candida utilis, 14-19% of the enterococcus faecium and 16-21% of the actinomycete;
in the fourth step, the solid lactic acid bacteria, the bacillus subtilis, the thiobacillus denitrificans, the candida utilis, the enterococcus faecium and the actinomycete obtained in the third step are put into a liquid stirrer to be stirred to obtain mixed bacterial colonies, and the bacterial colonies are uniformly stirred in the stirring process without obvious blocks;
in the fifth step, the mixed bacterial colony obtained in the fourth step is placed in a low-temperature environment for low-temperature drying, the water content after drying is 6%, and the preparation is obtained after drying;
in the sixth step, the preparation obtained in the fifth step is packaged, the appearance of the packaging bag is observed to be complete, and the preparation qualified in quality inspection is stored at low temperature.
3. The process for preparing a deodorizing microbial preparation for a breeding house according to claim 2, wherein the process comprises the following steps: in the second step, the temperature of damp-heat sterilization is 115 ℃, and the sterilization time is 20 min.
4. The process for preparing a deodorizing microbial preparation for a breeding house according to claim 2, wherein the process comprises the following steps: in the fourth step, the rotating speed of the liquid stirrer is 20-30 r/min.
5. The process for preparing a deodorizing microbial preparation for a breeding house according to claim 2, wherein the process comprises the following steps: in the fifth step, the low-temperature drying temperature is 4 ℃.
6. The process for preparing a deodorizing microbial preparation for a breeding house according to claim 2, wherein the process comprises the following steps: in the sixth step, the preservation temperature of the preparation is 4-10 ℃.
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