CN107779415A - A kind of complex micro organism fungicide of efficient degradation animal wastes - Google Patents
A kind of complex micro organism fungicide of efficient degradation animal wastes Download PDFInfo
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- CN107779415A CN107779415A CN201610785674.6A CN201610785674A CN107779415A CN 107779415 A CN107779415 A CN 107779415A CN 201610785674 A CN201610785674 A CN 201610785674A CN 107779415 A CN107779415 A CN 107779415A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The present invention relates to a kind of complex micro organism fungicide of efficient degradation animal wastes, mixed by bacillus subtilis, candida utili bacterium, aspergillus oryzae, Pediococcus acidilactici, lactobacillus acidophilus.The beneficial effects of the invention are as follows：Microbial bacteria agent prescription of the present invention is reasonable, it is easy to use, high-efficiency fermenting can be carried out to animal wastes, decomposed the protein of simultaneously synthesized micro-organism itself, decomposable process will not cause secondary pollution, effectively reduce ammonia, nitrogen etc. in animal-breeding field to discharge containing gas frowzy, avoid plant from rinsing secondary pollution of the sewage of animal wastes discharge to environment, so as to realize animal fecaluria to zero environmental zero-emission.
Description
Technical field
The present invention relates to a kind of complex micro organism fungicide of efficient degradation animal wastes, belong to microbial technology field.
Background technology
Combined according to Environmental Protection Department, State Statistics Bureau, the Ministry of Agriculture on 2 9th, 2010 and issued《The whole nation is dirty for the first time
Generally investigate publication in dye source》It has been shown that, in agricultural sources pollution, than it is more prominent be livestock and poultry breeding industry pollution problem, livestock and poultry breeding industry
COD, total nitrogen and total phosphorus discharge account for 96%, 38% and the 56% of agricultural sources respectively.In recent years, China's livestock breeding industry
Development it is swift and violent, so as to cause the pollution summation load of animal excrement and urine waste to exceed industrial wastewater and sanitary sewage summation.With
Area of the aquaculture as pillar industry, environment and economic benefit turn into the game between fish and bear's paw.How effectively to solve to support
The pollution problem of industry is grown, while and can takes into account economic development, turns into aquaculture urgent problem to be solved.
The content of the invention
It is an object of the invention to provide a kind of complex micro organism fungicide of efficient degradation animal wastes and degraded animal excreta
Just method, to prepare organic fertilizer.
The technical scheme that the present invention takes is as follows：
A kind of complex micro organism fungicide of efficient degradation animal wastes, by bacillus subtilis, candida utili bacterium, rice
Aspergillus, Pediococcus acidilactici, lactobacillus acidophilus composition.
The complex micro organism fungicide each component volume fraction of the efficient degradation animal wastes is：Bacillus subtilis
20%th, candida utili bacterium 20%, aspergillus oryzae 20%, Pediococcus acidilactici 20%, lactobacillus acidophilus 20%.
The preparation method of the complex micro organism fungicide of the efficient degradation animal wastes, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, bacillus subtilis nutrient broth respectively
Agar medium, candida utili corn Tween 80 agar medium, aspergillus oryzae sabouraud's agar, lactic acid sheet ball
Bacterium and lactobacillus acidophilus MRS agar mediums, respectively obtain generation bacterial strain；
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions；
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the efficient degradation animal wastes after being mixed by volume equal proportion
Complex micro organism fungicide.
28-32 DEG C of the step 1 activation culture temperature, incubation time 2-5 days.
It is 28-37 DEG C that the step 2, which expands cultivation temperature, incubation time 2-5 days.
A kind of method of efficient degradation animal wastes, including step is：
(1) pretreatment of animal wastes：Animal wastes are mixed with appropriate corn flour, dregs of beans, make system content moisture
40%~60%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg plan fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.0-1.5L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 7-10 days, when temperature is 10~20 DEG C, fermentation produces organic fertilizer in 10~15 days
Material.
The weight of described plan fermentation substrate is than composition：Animal wastes 95%, corn flour 3%, dregs of beans 2%.
Beneficial effects of the present invention：The present invention is by bacillus subtilis, candida utili bacterium, aspergillus oryzae, lactic acid sheet ball
Bacterium, lactobacillus acidophilus mix.The microbial bacteria agent prescription of the present invention is reasonable, easy to use, and animal wastes can be carried out
High-efficiency fermenting, is decomposed and the protein of synthesized micro-organism itself, decomposable process will not cause secondary pollution, is effectively reduced dynamic
Ammonia, nitrogen etc. discharge containing gas frowzy in thing plant, avoid plant from rinsing the sewage of animal wastes discharge to environment
Secondary pollution, so as to realize animal fecaluria to zero environmental zero-emission.
Embodiment
In order that those skilled in the art more understand technical scheme and determine the technique effect of the present invention, first
Preferred embodiment is provided, but following preferred embodiments are not limited to or relied in the realization of the present invention.
Embodiment 1
A kind of complex micro organism fungicide of efficient degradation animal wastes, by bacillus subtilis, candida utili bacterium, rice
Aspergillus, Pediococcus acidilactici, lactobacillus acidophilus composition.
The complex micro organism fungicide each component volume fraction of the efficient degradation animal wastes is：Bacillus subtilis
20%th, candida utili bacterium 20%, aspergillus oryzae 20%, Pediococcus acidilactici 20%, lactobacillus acidophilus 20%.
The preparation method of the complex micro organism fungicide of the efficient degradation animal wastes, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, bacillus subtilis nutrient broth respectively
Agar medium, candida utili corn Tween 80 agar medium, aspergillus oryzae sabouraud's agar, lactic acid sheet ball
Bacterium and lactobacillus acidophilus MRS agar mediums, respectively obtain generation bacterial strain；
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions；
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the efficient degradation animal wastes after being mixed by volume equal proportion
Complex micro organism fungicide.
28 DEG C of the step 1 activation culture temperature, incubation time 2 days.
It is 28 DEG C that the step 2, which expands cultivation temperature, incubation time 5 days.
A kind of method of efficient degradation animal wastes, including step is：
(1) pretreatment of animal wastes：Animal wastes are mixed with appropriate corn flour, dregs of beans, make system content moisture
40%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg plan fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.0L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 7-10 days, when temperature is 10~20 DEG C, fermentation produces organic fertilizer in 10~15 days
Material.
Embodiment 2
A kind of complex micro organism fungicide of efficient degradation animal wastes, by bacillus subtilis, candida utili bacterium, rice
Aspergillus, Pediococcus acidilactici, lactobacillus acidophilus composition.
The complex micro organism fungicide each component volume fraction of the efficient degradation animal wastes is：Bacillus subtilis
20%th, candida utili bacterium 20%, aspergillus oryzae 20%, Pediococcus acidilactici 20%, lactobacillus acidophilus 20%.
The preparation method of the complex micro organism fungicide of the efficient degradation animal wastes, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, bacillus subtilis nutrient broth respectively
Agar medium, candida utili corn Tween 80 agar medium, aspergillus oryzae sabouraud's agar, lactic acid sheet ball
Bacterium and lactobacillus acidophilus MRS agar mediums, respectively obtain generation bacterial strain；
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions；
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the efficient degradation animal wastes after being mixed by volume equal proportion
Complex micro organism fungicide.
32 DEG C of the step 1 activation culture temperature, incubation time 5 days.
It is 37 DEG C that the step 2, which expands cultivation temperature, incubation time 5 days.
A kind of method of efficient degradation animal wastes, including step is：
(1) pretreatment of animal wastes：Animal wastes are mixed with appropriate corn flour, dregs of beans, make system content moisture
60%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg plan fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.5L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 7-10 days, when temperature is 10~20 DEG C, fermentation produces organic fertilizer in 10~15 days
Material.
The weight of described plan fermentation substrate is than composition：Animal wastes 95%, corn flour 3%, dregs of beans 2%.
Embodiment 3
A kind of complex micro organism fungicide of efficient degradation animal wastes, by bacillus subtilis, candida utili bacterium, rice
Aspergillus, Pediococcus acidilactici, lactobacillus acidophilus composition.
The complex micro organism fungicide each component volume fraction of the efficient degradation animal wastes is：Bacillus subtilis
20%th, candida utili bacterium 20%, aspergillus oryzae 20%, Pediococcus acidilactici 20%, lactobacillus acidophilus 20%.
The preparation method of the complex micro organism fungicide of the efficient degradation animal wastes, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, bacillus subtilis nutrient broth respectively
Agar medium, candida utili corn Tween 80 agar medium, aspergillus oryzae sabouraud's agar, lactic acid sheet ball
Bacterium and lactobacillus acidophilus MRS agar mediums, respectively obtain generation bacterial strain；
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, to be expanded
Culture obtains five kinds of bacterium solutions；
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain the efficient degradation animal wastes after being mixed by volume equal proportion
Complex micro organism fungicide.
30 DEG C of the step 1 activation culture temperature, incubation time 4 days.
It is 32.5 DEG C that the step 2, which expands cultivation temperature, incubation time 4 days.
A kind of method of efficient degradation animal wastes, including step is：
(1) pretreatment of animal wastes：Animal wastes are mixed with appropriate corn flour, dregs of beans, make system content moisture
50%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg plan fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.25L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering is thin
Film, when temperature is 25 DEG C~36 DEG C, ferment 7-10 days, when temperature is 10~20 DEG C, fermentation produces organic fertilizer in 10~15 days
Material.
The weight of described plan fermentation substrate is than composition：Animal wastes 95%, corn flour 3%, dregs of beans 2%.
Complex micro organism fungicide of efficient degradation animal wastes described above and preparation method thereof and fermentation process, meet this
The proportion of the microorganism agent is invented, and the action effect of the microbial bacterial agent can be reached.
Claims (7)
1. the present invention relates to a kind of complex micro organism fungicide of efficient degradation animal wastes, by bacillus subtilis, Candida utilis
Saccharomycete, aspergillus oryzae, Pediococcus acidilactici, lactobacillus acidophilus composition.
2. the complex micro organism fungicide of a kind of efficient degradation animal wastes according to claim 1, it is characterised in that described
The complex micro organism fungicide each component volume fraction of efficient degradation animal wastes is：Bacillus subtilis 20%, Candida utilis ferment
Female bacterium 20%, aspergillus oryzae 20%, Pediococcus acidilactici 20%, lactobacillus acidophilus 20%.
3. the preparation method of the complex micro organism fungicide of efficient degradation animal wastes according to claim 1, including step is：
Step 1：Five kinds of strains are inoculated into activation culture in corresponding culture medium, bacillus subtilis nutrient broth agar respectively
Culture medium, candida utili corn Tween 80 agar medium, aspergillus oryzae sabouraud's agar, Pediococcus acidilactici and
Lactobacillus acidophilus MRS agar mediums, respectively obtain generation bacterial strain；
Step 2：Five kinds of generation strains that step 1 is obtained, which are individually inoculated into nutrient broth agar culture medium, expands culture
Obtain five kinds of bacterium solutions；
Step 3：Five kinds of bacterium solutions obtained by step 2 obtain answering for the efficient degradation animal wastes after being mixed by volume equal proportion
Close microbial bacterial agent.
4. a kind of preparation method of the complex micro organism fungicide of efficient degradation animal wastes according to claim 3, it is special
Sign is, 28-32 DEG C of the step 1 activation culture temperature, incubation time 2-5 days.
5. a kind of preparation method of the complex micro organism fungicide of efficient degradation animal wastes according to claim 3, it is special
Sign is that it is 28-37 DEG C that the step 2, which expands cultivation temperature, incubation time 2-5 days.
6. a kind of method of efficient degradation animal wastes, including step is：
(1) pretreatment of animal wastes：Animal wastes are mixed with appropriate corn flour, dregs of beans, system content moisture is existed
40%~60%, it is made and intends fermentation substrate；
(2) work seed bacterium solution：Microbial bacterial agent is inoculated into by every 100kg plan fermentation substrates with 0.15L dosage and contained
Activated in the mixed aqueous solution 1.0-1.5L of 0.85% salt and 1% glucose, work seed bacterium solution is made；
(3) mix and intend fermentation substrate：Above-mentioned work seed bacterium solution is well mixed with intending fermentation substrate, and plastic covering film,
When temperature is 25 DEG C~36 DEG C, ferment 7-10 days, when temperature is 10~20 DEG C, fermentation produces organic fertilizer in 10~15 days.
7. the method for a kind of efficient degradation animal wastes according to claim 6, it is characterised in that described sends out ferment bottom
The weight of thing is than composition：Animal wastes 95%, corn flour 3%, dregs of beans 2%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439610A (en) * | 2018-11-16 | 2019-03-08 | 安徽骏旌环保科技有限公司 | A kind of human feces decomposition culture medium |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN113088464A (en) * | 2021-03-26 | 2021-07-09 | 张掖兰标生物科技有限公司 | Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof |
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| CN101905985A (en) * | 2010-07-30 | 2010-12-08 | 河北省微生物研究所 | Method for preparing microorganism-decomposing agent used for producing biological organic fertilizer by livestock and poultry manure fermentation |
| CN103146606A (en) * | 2013-03-01 | 2013-06-12 | 贵州省畜牧兽医研究所 | Microbial agent for high-efficiency straw fermentation |
| CN103374523A (en) * | 2012-04-13 | 2013-10-30 | 蒋爱国 | Fermentation bed compound microorganism capable of rapidly decomposing animal waste |
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2016
- 2016-08-30 CN CN201610785674.6A patent/CN107779415A/en active Pending
Patent Citations (4)
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| CN101905985A (en) * | 2010-07-30 | 2010-12-08 | 河北省微生物研究所 | Method for preparing microorganism-decomposing agent used for producing biological organic fertilizer by livestock and poultry manure fermentation |
| CN103374523A (en) * | 2012-04-13 | 2013-10-30 | 蒋爱国 | Fermentation bed compound microorganism capable of rapidly decomposing animal waste |
| CN103146606A (en) * | 2013-03-01 | 2013-06-12 | 贵州省畜牧兽医研究所 | Microbial agent for high-efficiency straw fermentation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN109439610A (en) * | 2018-11-16 | 2019-03-08 | 安徽骏旌环保科技有限公司 | A kind of human feces decomposition culture medium |
| CN113088464A (en) * | 2021-03-26 | 2021-07-09 | 张掖兰标生物科技有限公司 | Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof |
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Application publication date: 20180309 |