CN109439610A - A kind of human feces decomposition culture medium - Google Patents
A kind of human feces decomposition culture medium Download PDFInfo
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- CN109439610A CN109439610A CN201811366251.6A CN201811366251A CN109439610A CN 109439610 A CN109439610 A CN 109439610A CN 201811366251 A CN201811366251 A CN 201811366251A CN 109439610 A CN109439610 A CN 109439610A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of human feces to decompose culture medium, and raw material includes: 20-30 portions of corn flour, 15-25 parts of mulberry leaf powders, 6-12 parts of dehydrated potato powders, 2-6 parts of charcoals, 5-10 parts of plant ash, 3-7 portions of bagasse, 2-4 portions of sucrose, 3-5 parts of boraxs, 1-3 parts of amino acid, 0.3-0.8 parts of glucose, 0.6-1.0 parts of magnesium sulfate, 1-2 parts of dipotassium hydrogen phosphates, 6-10 parts of agar, 10-16 parts of complex micro organism fungicides, 50-90 parts of water by weight.Excrement of the invention decomposes culture medium by scientific and reasonable formula and proportion, so that the complex micro organism fungicide speed of growth with higher and decomposition efficiency, can effectively improve the decomposition efficiency to organic matter, realizes innoxious, the recycling treatment of excrement.
Description
Technical field
The present invention relates to microorganisms technical fields, and in particular to a kind of human feces decomposition culture medium.
Background technique
Bio-toilet is one kind in environment protection water closet, refers to have and does not pollute the environment, and can be made full use of each
Kind resource, emphasizes a kind of lavatory of pollutant self-cleaning and resource circulation utilization concept and function.Current bio-toilet utilization rate
Most is the lavatory that microorganism fungus kind decomposes excrement, it has macromolecular available in excrement using its growth and breeding activity
Machine compound carries out biodegrade and is converted into Fungal biodiversity, and emulative inhibition simultaneously kills the micro- life of the pathogenicity in excrement
Object realizes innoxious, the recycling treatment of excrement.The function that zero-emission can be reached does not cause any pollution to environment completely.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of human feces to decompose culture medium, can promote
The fast-growth of complex micro organism fungicide can also carry out biodegrade to the organic compound in human feces and inhibit in excrement
Pathogenic microorganism growth, realize the harmless processing of excrement.
The invention proposes a kind of human feces decompose culture medium, raw material include: by weight 20-30 portions of corn flour,
15-25 parts of mulberry leaf powders, 6-12 parts of dehydrated potato powders, 2-6 parts of charcoals, 5-10 parts of plant ash, 3-7 portions of bagasse, 2-4 portions of sucrose,
3-5 parts of boraxs, 1-3 parts of amino acid, 0.3-0.8 parts of glucose, 0.6-1.0 parts of magnesium sulfate, 1-2 parts of dipotassium hydrogen phosphates, 6-10 parts
Agar, 10-16 part complex micro organism fungicide, 60-120 parts of water.
Preferably, the complex micro organism fungicide include bacillus, it is probiotics, lactobacillus acidophilus, enterococcus faecalis, thermophilic
Streptococcus, saccharomyces cerevisiae, aspergillus oryzae.
Preferably, the complex micro organism fungicide includes: 12-20% bacillus, 10-18% prebiotic by weight percentage
Bacterium, 10-16% lactobacillus acidophilus, 11-15% enterococcus faecalis, 10-14% streptococcus thermophilus, 5-9% saccharomyces cerevisiae, surplus are rice
Aspergillus.
Preferably, the corn flour, mulberry leaf powder, dehydrated potato powder, charcoal, plant ash, bagasse, complex micro organism fungicide
Weight ratio be 22-28:17-24:8-11:3-5:6-9:4-6:11-15.
Preferably, the sucrose, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar, complex microorganism
The weight ratio of microbial inoculum is 2.5-3.5:3-4:2-3:0.4-0.7:0.7-0.8:1.2-2:7-9:11-15.
Preferably, the raw material of the charcoal in peanut shell, corn stover, mulberry tree branch, cocoanut shell, hazelnut shell extremely
Few one kind.
Preferably, the human feces decompose the preparation method of culture medium, include the following steps: to crush bagasse, mistake
50-100 mesh is mixed with corn flour, mulberry leaf powder, dehydrated potato powder, charcoal, plant ash, is stirred evenly, and water is added, and stirs 5-
10min adds sucrose, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar, stirs 15- at 70-85 DEG C
30min is cooled to room temperature, and complex micro organism fungicide is added, and is uniformly mixed.
The present invention can carry out biodegrade to the organic compound in human feces, promote complex micro organism fungicide itself
Growth, while may also suppress the growth of the pathogenic microorganism in excrement, realize the harmless processing of excrement, avoid making environment
At pollution;The present invention uses corn flour, mulberry leaf powder, dehydrated potato powder, bagasse, plant ash for the main composition of culture medium, addition
Sucrose, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate supplement the nutrients, to promote the growth of complex micro organism fungicide,
Charcoal is added, synergy is formed with other raw materials, so that complex micro organism fungicide fast-growth is at ecological bacterium colony, and then makes
Its efficiency with higher for decomposing human feces, realizes the harmless processing of excrement.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A kind of human feces decomposition culture medium, raw material includes: 30 portions of corn flour, 15 parts of mulberry leaf powders, 12 parts by weight
Dehydrated potato powder, 2 parts of charcoals, 10 parts of plant ash, 3 portions of bagasse, 4 portions of sucrose, 3 parts of boraxs, 3 parts of amino acid, 0.3 portion of grape
Sugar, 1.0 parts of magnesium sulfate, 1 part of dipotassium hydrogen phosphate, 10 parts of agar, 10 parts of complex micro organism fungicides, 120 parts of water;
Wherein, the complex micro organism fungicide includes: 12% bacillus, 18% probiotics, 10% by weight percentage
Lactobacillus acidophilus, 15% enterococcus faecalis, 10% streptococcus thermophilus, 9% saccharomyces cerevisiae, surplus are aspergillus oryzae;The charcoal
Raw material selects peanut shell;
The human feces decompose the preparation method of culture medium, include the following steps: to crush bagasse, cross 50 meshes,
It mixes, stirs evenly with corn flour, mulberry leaf powder, dehydrated potato powder, charcoal, plant ash, water is added, stir 10min, add sugarcane
Sugar, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar stir 30min at 70 DEG C, are cooled to room temperature, adds
Enter complex micro organism fungicide, is uniformly mixed.
Embodiment 2
A kind of human feces decomposition culture medium, raw material includes: 20 portions of corn flour, 25 parts of mulberry leaf powders, 6 portions of horses by weight
Bell potato powder, 6 parts of charcoals, 5 parts of plant ash, 7 portions of bagasse, 2 portions of sucrose, 5 parts of boraxs, 1 part of amino acid, 0.8 part of glucose,
0.6 part of magnesium sulfate, 2 parts of dipotassium hydrogen phosphates, 6 parts of agar, 16 parts of complex micro organism fungicides, 60 parts of water;
Wherein, the complex micro organism fungicide includes: 20% bacillus, 10% probiotics, 16% by weight percentage
Lactobacillus acidophilus, 11% enterococcus faecalis, 14% streptococcus thermophilus, 5% saccharomyces cerevisiae, surplus are aspergillus oryzae;The charcoal
Raw material selects corn stover;
The human feces decompose the preparation method of culture medium, include the following steps: to crush bagasse, sieve with 100 mesh sieve,
It mixes, stirs evenly with corn flour, mulberry leaf powder, dehydrated potato powder, charcoal, plant ash, water is added, stir 5min, add sugarcane
Sugar, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar stir 15min at 85 DEG C, are cooled to room temperature, adds
Enter complex micro organism fungicide, is uniformly mixed.
Embodiment 3
A kind of human feces decomposition culture medium, raw material includes: 28 portions of corn flour, 17 parts of mulberry leaf powders, 11 parts by weight
Dehydrated potato powder, 3 parts of charcoals, 9 parts of plant ash, 4 portions of bagasse, 3.5 portions of sucrose, 3 parts of boraxs, 3 parts of amino acid, 0.4 portion of grape
Sugar, 0.8 part of magnesium sulfate, 1.2 parts of dipotassium hydrogen phosphates, 9 parts of agar, 11 parts of complex micro organism fungicides, 100 parts of water;
Wherein, the complex micro organism fungicide includes: 14% bacillus, 16% probiotics, 13% by weight percentage
Lactobacillus acidophilus, 13% enterococcus faecalis, 11% streptococcus thermophilus, 8% saccharomyces cerevisiae, surplus are aspergillus oryzae;The charcoal
Raw material selects mulberry tree branch and cocoanut shell;
The human feces decompose the preparation method of culture medium, include the following steps: to crush bagasse, cross 60 meshes,
It mixes, stirs evenly with corn flour, mulberry leaf powder, dehydrated potato powder, charcoal, plant ash, water is added, stir 9min, add sugarcane
Sugar, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar stir 25min at 75 DEG C, are cooled to room temperature, adds
Enter complex micro organism fungicide, is uniformly mixed.
Embodiment 4
A kind of human feces decomposition culture medium, raw material includes: 22 portions of corn flour, 24 parts of mulberry leaf powders, 8 portions of horses by weight
Bell potato powder, 5 parts of charcoals, 6 parts of plant ash, 6 portions of bagasse, 2.5 portions of sucrose, 4 parts of boraxs, 2 parts of amino acid, 0.7 part of glucose,
0.7 part of magnesium sulfate, 2 parts of dipotassium hydrogen phosphates, 7 parts of agar, 15 parts of complex micro organism fungicides, 75 parts of water;
Wherein, the complex micro organism fungicide includes: 18% bacillus, 12% probiotics, 15% by weight percentage
Lactobacillus acidophilus, 12% enterococcus faecalis, 13% streptococcus thermophilus, 6% saccharomyces cerevisiae, surplus are aspergillus oryzae;The charcoal
Raw material selects peanut shell and hazelnut shell;
The human feces decompose the preparation method of culture medium, include the following steps: to crush bagasse, cross 80 meshes,
It mixes, stirs evenly with corn flour, mulberry leaf powder, dehydrated potato powder, charcoal, plant ash, water is added, stir 6min, add sugarcane
Sugar, borax, amino acid, glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar stir 20min at 80 DEG C, are cooled to room temperature, adds
Enter complex micro organism fungicide, is uniformly mixed.
Test example 1
The human feces being respectively adopted in 1-4 of the embodiment of the present invention decompose culture medium and mankind's liquid manure by weight 1:300
Mixing carries out fermentation test, and test result is as shown in table 1.
Table 1
Project | Organic matter decomposition rate (%) | Deodorizing rate (%) |
Embodiment 1 | 93 | 90 |
Embodiment 2 | 95 | 89 |
Embodiment 3 | 96 | 92 |
Embodiment 4 | 97 | 94 |
As can be seen from Table 1, human feces of the invention decompose culture medium, and the effect with higher for decomposing excrement is real
Innoxious, the recycling treatment of existing excrement, do not pollute the environment.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of human feces decompose culture medium, which is characterized in that its raw material includes: 20-30 parts of corn flour, 15- by weight
25 parts of mulberry leaf powders, 6-12 parts of dehydrated potato powders, 2-6 parts of charcoals, 5-10 parts of plant ash, 3-7 portions of bagasse, 2-4 parts of sucrose, 3-5
Part borax, 1-3 parts of amino acid, 0.3-0.8 parts of glucose, 0.6-1.0 parts of magnesium sulfate, 1-2 parts of dipotassium hydrogen phosphates, 6-10 parts of fine jades
Rouge, 10-16 part complex micro organism fungicide, 60-120 parts of water.
2. human feces according to claim 1 decompose culture medium, which is characterized in that the complex micro organism fungicide includes
Bacillus, probiotics, lactobacillus acidophilus, enterococcus faecalis, streptococcus thermophilus, saccharomyces cerevisiae, aspergillus oryzae.
3. human feces according to claim 1 decompose culture medium, which is characterized in that the complex micro organism fungicide is by weight
Amount percentage include: 12-20% bacillus, 10-18% probiotics, 10-16% lactobacillus acidophilus, 11-15% enterococcus faecalis,
10-14% streptococcus thermophilus, 5-9% saccharomyces cerevisiae, surplus are aspergillus oryzae.
4. human feces according to claim 1 decompose culture medium, which is characterized in that the corn flour, mulberry leaf powder, Ma Ling
Potato powder, charcoal, plant ash, bagasse, complex micro organism fungicide weight ratio be 22-28:17-24:8-11:3-5:6-9:4-
6:11-15.
5. human feces according to claim 1 decompose culture medium, which is characterized in that the sucrose, borax, amino acid,
Glucose, magnesium sulfate, dipotassium hydrogen phosphate, agar, complex micro organism fungicide weight ratio be 2.5-3.5:3-4:2-3:0.4-
0.7:0.7-0.8:1.2-2:7-9:11-15.
6. human feces according to claim 1 decompose culture medium, which is characterized in that the raw material of the charcoal is selected from flower
At least one of raw shell, corn stover, mulberry tree branch, cocoanut shell, hazelnut shell.
7. human feces according to claim 1 decompose culture medium, which is characterized in that the human feces decompose culture medium
Preparation method, include the following steps: to crush bagasse, cross 50-100 mesh, with corn flour, mulberry leaf powder, dehydrated potato powder, life
Object charcoal, plant ash mixing, stir evenly, water are added, stir 5-10min, add sucrose, borax, amino acid, glucose, sulphur
Sour magnesium, dipotassium hydrogen phosphate, agar stir 15-30min at 70-85 DEG C, are cooled to room temperature, and complex micro organism fungicide is added, and mix
It closes uniform.
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CN201811366251.6A CN109439610A (en) | 2018-11-16 | 2018-11-16 | A kind of human feces decomposition culture medium |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109880773A (en) * | 2019-03-29 | 2019-06-14 | 上海华杰生态环境工程有限公司 | The complex microorganism preparations and preparation method thereof of efficient-decomposition human and animal excreta |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101591624A (en) * | 2009-05-08 | 2009-12-02 | 上海华杰生态环境工程有限公司 | A kind of preparation method who handles the effectively compound microbial inoculant of human and animal excreta |
CN106995788A (en) * | 2016-01-22 | 2017-08-01 | 江苏佳净洁环境科技有限公司 | A kind of complex microorganism preparations and preparation method and application for decomposing human feces |
CN107779415A (en) * | 2016-08-30 | 2018-03-09 | 蒋爱国 | A kind of complex micro organism fungicide of efficient degradation animal wastes |
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2018
- 2018-11-16 CN CN201811366251.6A patent/CN109439610A/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101591624A (en) * | 2009-05-08 | 2009-12-02 | 上海华杰生态环境工程有限公司 | A kind of preparation method who handles the effectively compound microbial inoculant of human and animal excreta |
CN106995788A (en) * | 2016-01-22 | 2017-08-01 | 江苏佳净洁环境科技有限公司 | A kind of complex microorganism preparations and preparation method and application for decomposing human feces |
CN107779415A (en) * | 2016-08-30 | 2018-03-09 | 蒋爱国 | A kind of complex micro organism fungicide of efficient degradation animal wastes |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109880773A (en) * | 2019-03-29 | 2019-06-14 | 上海华杰生态环境工程有限公司 | The complex microorganism preparations and preparation method thereof of efficient-decomposition human and animal excreta |
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Application publication date: 20190308 |