CN105669260A - Preparation method of bioorganic fertilizer - Google Patents

Preparation method of bioorganic fertilizer Download PDF

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Publication number
CN105669260A
CN105669260A CN201511028239.0A CN201511028239A CN105669260A CN 105669260 A CN105669260 A CN 105669260A CN 201511028239 A CN201511028239 A CN 201511028239A CN 105669260 A CN105669260 A CN 105669260A
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China
Prior art keywords
fermentation
preparation
prepare
culture medium
substrate
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Pending
Application number
CN201511028239.0A
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Chinese (zh)
Inventor
林伟伟
林生
林文雄
吴林坤
陈军
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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Priority to CN201511028239.0A priority Critical patent/CN105669260A/en
Publication of CN105669260A publication Critical patent/CN105669260A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00Fertilisers from household or town refuse
    • C05F9/04Biological compost
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/30Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using irradiation, e.g. solar or nuclear radiation; using electric or magnetic fields
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a preparation method of a bioorganic fertilizer. The method comprises the following steps: 1, preparing a substrate: naturally solarizing or drying pig dung, carrying out ultraviolet lamp irradiation disinfection for 30-120min, and overturning the pig dung 2-3 times in the irritation process to prepare the fermentation substrate; 2, carrying out two stage fermentation: sterilizing the fermentation substrate at 121DEG C for 20-30min, cooling the sterilized substrate to room temperature, adding water, and uniformly stirring to prepare a solid fermentation medium, inoculating a lactobacillus strain liquid, carrying out anaerobic fermentation for 48h, inoculating a Candida utilis strain liquid, introducing aseptic air, and carrying out aerobic fermentation for 48-72h to obtain a fermentation product; 4, drying: drying the fermentation product at 55-60DEG C; and 5, proportioning: adding a biological regulator to the dried fermentation product according to a weight ratio of 100:(0.2-1) in order to prepare the bioorganic fertilizer, wherein the biological regulator is photosynthetic bacteria and azotobacteria. The bioorganic fertilizer prepared in the invention can greatly improve the output per 666.7m<2> of land of crops.

Description

A kind of preparation method of biological organic fertilizer
Technical field
The present invention relates to the technical field of fertilizer, the preparation method particularly relating to a kind of biological organic fertilizer.
Background technology
Fertilizer, is mainly derived from plant and (or) animal, imposes on the soil carbonaceous material to provide plant nutrient to be its major function. Through biological substance, animals and plants garbage, plant residue processing, eliminate poisonous and harmful substance therein, rich in a large amount of benefit materials, including: multiple organic acid, peptides and include the nutrient enriched of nitrogen, phosphorus, potassium. Full nutrition can not only be provided for crops, and fertilizer efficiency is long, can increase and update the soil organism, promote microbial reproduction, improve physicochemical property and the biological activity of soil, be the Main Nutrients of pollution-free food production.
Application number is: the patent of invention of 201310353254.7, discloses a kind of biological organic fertilizer, relates to agriculture field. Described biological organic fertilizer, adopts and prepares with the following method: by mud, pig manure and straw mixing after fermentation, obtain fermenting mixture; By described fermenting mixture dry after mix with flyash, pulverize after namely obtain Agricultural organic fertilizer. The biological organic fertilizer of the offer of this invention, cost is low, fertilizer efficiency soon, easily absorb.
Application number is: the patent of invention of 201510235968.7, disclose a kind of organic fertilizer, specifically it is made up of following mass percent component: N P and K 5%, fermentation poultry dung 40-50%, fermentation cattle manure 20%, fermentation mushroom bran 10-20%, Maifanitum 15%, wherein the content of organic matter is more than the 45% of total nutrient. The organic fertilizer of this invention, rich in multiple organic matter, trace element, can improve soil, makes plant growth healthy and strong; After inorganic fertilizer compounding application, the utilization rate of inorganic fertilizer can be improved, reduce the consumption of inorganic fertilizer. The organic fertilizer purposes of the present invention is extremely wide, it is adaptable to any soil and crop.
Application number is: the patent of invention of 201410513798.X, disclose a kind of fertilizer, it is solid organic castoff, microbial fermentation inoculum and adjuvant is be uniformly mixed so as to obtain fermentation raw material at 70~75: 29.99~24.99: 0.01 by mass percentage, it is not less than 55 DEG C, the fertilizer that aerobic fermentation obtained no less than 7 days in temperature. The fertilizer of this invention, with fresh chicken manure for dominant fermentation raw material, take the aerobic continuous fermentation of pool, fermented raw material fully becomes thoroughly decomposed, decomposes, deodorization, sterilizing obtain product, wherein beneficial microbe can also secrete multiple metabolite, after contacting with root system of plant, it is possible to stimulates crop growth, regulate metabolism, improve the comprehensive resist ability of crops.
As it has been described above, in the preparation method of organic fertilizer of prior art, or complicated component, or fermentation technology is single, and the fertilizer prepared can not meet the needs of crop growth well. In view of this, the preparation method that the present inventor studies and devises a kind of biological organic fertilizer, this case thus produces.
Summary of the invention
The preparation method that it is an object of the invention to provide a kind of biological organic fertilizer, aerobic fermentation by the anaerobic fermentation of lactobacillus and Candida utilis, the compositions such as the macromole of pig manure organic matter are absorbed by the micro-organisms and convert the little molecular nutrition material such as organic acid, salt to, it is beneficial to the root absorption of crops, it is achieved high yield.
For achieving the above object, this invention address that the technical scheme of its technical problem is:
The preparation method of a kind of biological organic fertilizer, comprises the following steps:
Prepared by step one, substrate: naturally dried by pig manure or dry, and adopts ultra violet lamp sterilization 30-120min, during irradiation, stirs 2-3 time, prepare fermentation substrate;
Step 2, second order fermentation: by 121 DEG C of sterilizing 20-30min of above-mentioned fermentation substrate, be cooled to room temperature, adds water, stirring, prepare solid fermentation culture medium, the water content of solid fermentation culture medium is 5-10%, access the strain liquid of lactobacillus, first anaerobic fermentation 48h, fermentation temperature 25-35 DEG C; Access the strain liquid of Candida utilis again, and pass into filtrated air, aerobic fermentation 48-72h, fermentation temperature 25-45 DEG C; Prepare tunning;
Step 4, dry: tunning being dried, dries temperature 55-60 DEG C, after drying, water content is not higher than 8%;
Step 5, dispensing: the 100:(0.2-1 by weight ratio of tunning after the drying) ratio add biological regulator, described biological regulator is photosynthetic bacteria and nitrogen-fixing bacteria, prepare this biological organic fertilizer.
Optimal way as embodiment, the preparation of the strain liquid of described lactobacillus includes rejuvenation and the amplification culture of lactobacillus, the culture medium that described rejuvenation adopts includes casein peptone 10g/L, glucose 15g/L, dipotassium hydrogen phosphate 2g/L and magnesium sulfate 0.5g/L, and all the other components are water; Described rejuvenation culture medium pH6-7, rejuvenation temperature is 25-35 DEG C, rejuvenation time 24h.
Optimal way as embodiment, the preparation of the strain liquid of described Candida utilis includes strain recovery and amplification culture, the preparation method of the culture medium that the recovery of described strain adopts is: added by 200-300g Semen Maydis powder in distilled water, stir evenly, slow fire boils rear timing 1h, filtered through gauze, adds 15-20g agar post-heating and dissolves, and supplies the water yield to 1000mL. Subpackage, 121 DEG C of sterilizing 20min.
The culture medium adopted as the optimal way of embodiment, described lactobacillus and described Candida utilis amplification culture includes one or more of ground melon skin, distiller's grains of beer, tapioca starch and tea stalks respectively.
After the present invention adopts above-mentioned technical scheme, there is following beneficial effect:
1. take full advantage of pig manure, be greatly saved the consumed resource adopting industrial chemicals to manufacture chemical fertilizer, production technology, cleanliness without any pollution, environmental protection.
2. the method that the present invention adopts second order fermentation, aerobic fermentation by the anaerobic fermentation of lactobacillus and Candida utilis, fully by pig manure fermentation, by the primary metabolite of microorganism and cometabolism, the part macromole organic matter of pig manure is absorbed by the micro-organisms and converts the little molecular nutrition material such as organic acid, salt to, be beneficial to the root absorption of crops.
3., rich in substantial amounts of photosynthetic bacteria and nitrogen-fixing bacteria in this fertilizer, be conducive to the production needs with crops.
Detailed description of the invention
Embodiment 1
Prepared by step one, substrate: naturally dried by pig manure or dry, and adopts ultra violet lamp sterilization 30min, during irradiation, stirs 2 times, prepare fermentation substrate;
Step 2, second order fermentation: by 121 DEG C of sterilizing 20min of above-mentioned fermentation substrate, be cooled to room temperature, adds water, stirring, prepare solid fermentation culture medium, the water content of solid fermentation culture medium is 5%, access the strain liquid of lactobacillus, first anaerobic fermentation 48h, fermentation temperature 25 DEG C; Access the strain liquid of Candida utilis again, and pass into filtrated air, aerobic fermentation 48h, fermentation temperature 25 DEG C; Prepare tunning;
Step 4, dry: tunning being dried, dries temperature 55 DEG C, after drying, water content is not higher than 8%;
Step 5, dispensing: the ratio of the 100:0.2 by weight ratio of tunning after the drying adds biological regulator, and described biological regulator is photosynthetic bacteria and nitrogen-fixing bacteria, prepare this biological organic fertilizer.
Embodiment 2
Prepared by step one, substrate: naturally dried by pig manure or dry, and adopts ultra violet lamp sterilization 80min, during irradiation, stirs 3 times, prepare fermentation substrate;
Step 2, second order fermentation: by 121 DEG C of sterilizing 15min of above-mentioned fermentation substrate, be cooled to room temperature, adds water, stirring, prepare solid fermentation culture medium, the water content of solid fermentation culture medium is 8%, access the strain liquid of lactobacillus, first anaerobic fermentation 48h, fermentation temperature 30 DEG C; Access the strain liquid of Candida utilis again, and pass into filtrated air, aerobic fermentation 60h, fermentation temperature 30 DEG C; Prepare tunning;
Step 4, dry: tunning being dried, dries temperature 55 DEG C, after drying, water content is not higher than 8%;
Step 5, dispensing: the ratio of the 100:0.6 by weight ratio of tunning after the drying adds biological regulator, and described biological regulator is photosynthetic bacteria and nitrogen-fixing bacteria, prepare this biological organic fertilizer.
Embodiment 3
Prepared by step one, substrate: naturally dried by pig manure or dry, and adopts ultra violet lamp sterilization 120min, during irradiation, stirs 3 times, prepare fermentation substrate;
Step 2, second order fermentation: by 121 DEG C of sterilizing 30min of above-mentioned fermentation substrate, be cooled to room temperature, adds water, stirring, prepare solid fermentation culture medium, the water content of solid fermentation culture medium is 10%, access the strain liquid of lactobacillus, first anaerobic fermentation 48h, fermentation temperature 35 DEG C; Access the strain liquid of Candida utilis again, and pass into filtrated air, aerobic fermentation 72h, fermentation temperature 45 DEG C; Prepare tunning;
Step 4, dry: tunning being dried, dries temperature 60 C, after drying, water content is not higher than 8%;
Step 5, dispensing: the ratio of the 100:1 by weight ratio of tunning after the drying adds biological regulator, and described biological regulator is photosynthetic bacteria and nitrogen-fixing bacteria, prepare this biological organic fertilizer.
Optimal way as embodiment 1-3, the preparation of the strain liquid of described lactobacillus includes rejuvenation and the amplification culture of lactobacillus, the culture medium that described rejuvenation adopts includes casein peptone 10g/L, glucose 15g/L, dipotassium hydrogen phosphate 2g/L and magnesium sulfate 0.5g/L, and all the other components are water; Described rejuvenation culture medium pH6-7, rejuvenation temperature is 25-35 DEG C, rejuvenation time 24h.
Optimal way as embodiment 1-3, the preparation of the strain liquid of described Candida utilis includes strain recovery and amplification culture, the preparation method of the culture medium that the recovery of described strain adopts is: added by 200-300g Semen Maydis powder in distilled water, stir evenly, slow fire boils rear timing 1h, filtered through gauze, adds 15-20g agar post-heating and dissolves, and supplies the water yield to 1000mL. Subpackage, 121 DEG C of sterilizing 20min.
The culture medium adopted as the optimal way of embodiment 1-3, described lactobacillus and described Candida utilis amplification culture includes one or more of ground melon skin, distiller's grains of beer, tapioca starch and tea stalks respectively.
After milpa is applied fertilizer by the fertilizer adopting embodiment 1, relative to the milpa of traditional chemical fertilizer fertilising, when other the same terms are identical, per mu yield improves 30%; After milpa is applied fertilizer by the fertilizer adopting embodiment 2, when other the same terms are identical, relative to the milpa of traditional chemical fertilizer fertilising, per mu yield improves 35%; After milpa is applied fertilizer by the fertilizer adopting embodiment 3, when other the same terms are identical, relative to the milpa of traditional chemical fertilizer fertilising, per mu yield improves 32%
All deformation that those of ordinary skill in the art can directly derive from the disclosure of invention or associate, are all considered as protection scope of the present invention.

Claims (4)

1. the preparation method of a biological organic fertilizer, it is characterised in that: comprise the following steps:
Prepared by step one, substrate: naturally dried by pig manure or dry, and adopts ultra violet lamp sterilization 30-120min, during irradiation, stirs 2-3 time, prepare fermentation substrate;
Step 2, second order fermentation: by 121 DEG C of sterilizing 20-30min of above-mentioned fermentation substrate, be cooled to room temperature, adds water, stirring, prepare solid fermentation culture medium, the water content of solid fermentation culture medium is 5-10%, access the strain liquid of lactobacillus, first anaerobic fermentation 48h, fermentation temperature 25-35 DEG C; Access the strain liquid of Candida utilis again, and pass into filtrated air, aerobic fermentation 48-72h, fermentation temperature 25-45 DEG C; Prepare tunning;
Step 4, dry: tunning being dried, dries temperature 55-60 DEG C, after drying, water content is not higher than 8%;
Step 5, dispensing: the 100:(0.2-1 by weight ratio of tunning after the drying) ratio add biological regulator, described biological regulator is photosynthetic bacteria and nitrogen-fixing bacteria, prepare this biological organic fertilizer.
2. the preparation method of a kind of biological organic fertilizer as claimed in claim 1, it is characterized in that: the preparation of the strain liquid of described lactobacillus includes rejuvenation and the amplification culture of lactobacillus, the culture medium that described rejuvenation adopts includes casein peptone 10g/L, glucose 15g/L, dipotassium hydrogen phosphate 2g/L and magnesium sulfate 0.5g/L, and all the other components are water; Described rejuvenation culture medium pH6-7, rejuvenation temperature is 25-35 DEG C, rejuvenation time 24h.
3. the preparation method of a kind of biological organic fertilizer as claimed in claim 1, it is characterized in that: the preparation of the strain liquid of described Candida utilis includes strain recovery and amplification culture, the preparation method of the culture medium that the recovery of described strain adopts is: added by 200-300g Semen Maydis powder in distilled water, stir evenly, slow fire boils rear timing 1h, filtered through gauze, adds 15-20g agar post-heating and dissolves, and supplies the water yield to 1000mL. Subpackage, 121 DEG C of sterilizing 20min.
4. the preparation method of a kind of biological organic fertilizer as claimed in claim 1, it is characterised in that: the culture medium that described lactobacillus and described Candida utilis amplification culture adopt includes one or more of ground melon skin, distiller's grains of beer, tapioca starch and tea stalks respectively.
CN201511028239.0A 2015-12-31 2015-12-31 Preparation method of bioorganic fertilizer Pending CN105669260A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107616050A (en) * 2017-09-13 2018-01-23 界首市汉武家庭农场 A kind of potted Arabian jasmine four seasons management method
CN107640984A (en) * 2017-09-21 2018-01-30 广西吉朋投资有限公司 A kind of processing method of chicken manure
CN108586148A (en) * 2018-06-25 2018-09-28 天柱县森源米业有限责任公司 A kind of Rice Cropping dedicated fertilizer
CN108794256A (en) * 2018-06-25 2018-11-13 天柱县森源米业有限责任公司 A kind of preparation method of Rice Cropping dedicated fertilizer
CN113087557A (en) * 2021-04-07 2021-07-09 葛成娟 Organic fertilizer fermentor and process thereof
CN115259961A (en) * 2022-08-08 2022-11-01 广东省适然环境科技研究有限公司 Method for treating organic fertilizer by horizontal fermentation tank

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1974492A (en) * 2006-12-11 2007-06-06 陈南岭 Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer
CN103086767A (en) * 2013-01-25 2013-05-08 福建省星源农牧科技股份有限公司 High-potassium organic composite fertilizer special for dragon fruit and production method using domestic sludge
CN105152743A (en) * 2015-07-22 2015-12-16 靖边县辰田生物科技有限公司 Preparation method for grass carbon compound biofertilizer
CN105198504A (en) * 2015-11-04 2015-12-30 西南科技大学 Microbial fermentation organic fertilizer and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1974492A (en) * 2006-12-11 2007-06-06 陈南岭 Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer
CN103086767A (en) * 2013-01-25 2013-05-08 福建省星源农牧科技股份有限公司 High-potassium organic composite fertilizer special for dragon fruit and production method using domestic sludge
CN105152743A (en) * 2015-07-22 2015-12-16 靖边县辰田生物科技有限公司 Preparation method for grass carbon compound biofertilizer
CN105198504A (en) * 2015-11-04 2015-12-30 西南科技大学 Microbial fermentation organic fertilizer and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107616050A (en) * 2017-09-13 2018-01-23 界首市汉武家庭农场 A kind of potted Arabian jasmine four seasons management method
CN107640984A (en) * 2017-09-21 2018-01-30 广西吉朋投资有限公司 A kind of processing method of chicken manure
CN108586148A (en) * 2018-06-25 2018-09-28 天柱县森源米业有限责任公司 A kind of Rice Cropping dedicated fertilizer
CN108794256A (en) * 2018-06-25 2018-11-13 天柱县森源米业有限责任公司 A kind of preparation method of Rice Cropping dedicated fertilizer
CN113087557A (en) * 2021-04-07 2021-07-09 葛成娟 Organic fertilizer fermentor and process thereof
CN115259961A (en) * 2022-08-08 2022-11-01 广东省适然环境科技研究有限公司 Method for treating organic fertilizer by horizontal fermentation tank

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