CN113088464A - Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof - Google Patents
Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof Download PDFInfo
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- CN113088464A CN113088464A CN202110324910.5A CN202110324910A CN113088464A CN 113088464 A CN113088464 A CN 113088464A CN 202110324910 A CN202110324910 A CN 202110324910A CN 113088464 A CN113088464 A CN 113088464A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/02—Treatment of water, waste water, or sewage by heating
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The high-efficiency microbial composite microbial inoculum for decomposing and treating feces and a preparation method thereof, which consists of saccharomycetes, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis; the mass ratio of the microorganisms is saccharomycetes, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis is 2:3:1:1:1.5:1:2.5: 2; the preparation method comprises the following steps: (1) respectively breeding mother bacteria, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis which are screened from nature to a high-density state through rejuvenation; (2) and (2) mixing the microorganisms in the step (1) according to the mass ratio of 2:3:1:1:1.5:1:2.5:2 to form the target compound microbial agent. The high-efficiency microbial composite inoculant has the characteristics of complex composition, stable structure and wide functions, can realize harmless treatment of excrement with high efficiency and low consumption, and solves the problems in the conventional excrement treatment process.
Description
Technical Field
The invention relates to the technical field of microbial agent preparation and fecal sewage decomposition treatment, in particular to a high-efficiency microbial compound microbial agent for fecal sewage decomposition and treatment and a preparation method thereof.
Background
The existing ecological toilet with the highest utilization rate is a toilet with excrement decomposed by microorganisms, and the available macromolecular organic compounds in the excrement are biodegraded and converted into bacterial biomass by using growth and reproduction activities, so that pathogenic microorganisms in the excrement are competitively inhibited and killed, odor substances generated in the excrement are adsorbed, degraded and converted, harmless recycling treatment of the excrement is realized, and zero emission is achieved without causing any pollution to the environment. Meanwhile, the high-efficiency bioactive filler containing a large amount of aerobic microorganisms can absorb and convert odor in the excrement and urine, and the decomposition, deodorization and purification integrated treatment of the excrement is realized. And harmful bacteria such as escherichia coli, parasitic ova and the like and miscellaneous bacteria in the excrement are killed or phagocytosed by microbial communities at the high temperature of more than 60 ℃, so that safety, sanitation and harmlessness are realized. However, the present invention has been made to solve the above problems by devising the conventional complex microorganisms to lose their activity in a high temperature environment.
Disclosure of Invention
The invention aims to provide a high-efficiency microbial compound microbial inoculum for decomposing and treating feces.
In order to achieve the purpose, the invention adopts the following technical scheme:
a high-efficiency composite microbial inoculum for decomposing and treating feces is prepared from yeast, lactobacillus, Bacillus subtilis, Aspergillus niger, Aspergillus oryzae, Rhizopus, and Bacillus licheniformis.
Further, the mass ratio of the microorganisms is saccharomycetes, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis is 2:3:1:1:1.5:1:2.5: 2.
The invention also aims to provide a preparation method of the high-efficiency microbial compound inoculant for decomposing and treating the feces.
In order to achieve the purpose, the invention adopts the following technical scheme:
the preparation method of the efficient microbial compound inoculant for decomposing and treating the feces comprises the following steps:
(1) respectively breeding mother bacteria, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis which are screened from nature to a high-density state by rejuvenation, wherein OD600 is more than 1;
(2) and (2) mixing the microorganisms in the step (1) according to the mass ratio of 2:3:1:1:1.5:1:2.5:2 to form the target compound microbial agent.
Further, the preparation method of the solidified microorganism composite microbial inoculum of the high-efficiency microorganism composite microbial inoculum for decomposing and treating the feces comprises the following steps:
processing wheat bran by an ultrafine grinder to prepare fine bran, mixing the fine bran with the efficient microbial compound inoculum for decomposing and processing the feces, then oscillating in an oscillating constant-temperature incubator for 2-4 days, and standing or centrifugally separating solid components to obtain the solidified microbial compound inoculum.
Further, according to the preparation method of the solidified microbial composite inoculant for the efficient microbial composite inoculant for decomposing and treating the feces, 50 ml of nutrient substance mixed liquor required by microbial flora is added into every 200 g of the solidified microbial composite inoculant.
Further, the preparation method of the solidified microbial composite inoculant for the efficient microbial composite inoculant for decomposing and treating the feces comprises the step of mixing industrial brown sugar, antibiotics and amino acid solution according to the mass ratio of 500:1: 1.
Furthermore, the preparation method of the solidified microorganism composite microbial inoculum of the high-efficiency microorganism composite microbial inoculum for decomposing and treating the feces needs to add a filler carrier during use.
Furthermore, in the preparation method of the solidified microorganism composite microbial inoculum of the efficient microorganism composite microbial inoculum for decomposing and treating the feces, the filler carrier is a substance with stronger water absorption.
Further, according to the preparation method of the solidified microbial composite inoculant for the efficient microbial composite inoculant for decomposing and treating the feces, the substances with strong water absorption are sawdust and sawdust, the sawdust and the sawdust are soft in texture, high in water absorption amount and capable of effectively absorbing malodorous components, and the sawdust with impurities and chemical residues can be used only after being aired, cleaned and dried.
The invention has the beneficial effects that:
1. the synergistic effect of different microorganisms in the high-efficiency microbial compound inoculant can effectively adjust the internal flora structure, and avoid the problems of long fermentation period and low composting efficiency in the process of applying the microbial inoculant with single component to manure composting.
2. The carrier is utilized to solidify the compound microbial inoculum, and required nutrient substances are added, so that the solid microbial inoculum is easier to store and transport compared with the traditional liquid microbial inoculum, the microorganisms in the solid microbial inoculum are easier to survive, the storage time is longer, and the solid microbial inoculum can be applied to large-scale production and application.
3. Compared with a method for directly adding a microbial inoculum, the immobilized microbial technology fixes thalli in bran, so that the influence of the microbes impacted by external factors is reduced, the activity is high, the density is high, certain stability is kept, the thalli are continuously proliferated and diffused outwards in vivo, the loss of original thalli is avoided, and the treatment efficiency of excrement is improved.
4. Wheat bran is a byproduct generated in the production process of wheat, has wide source and low price, but has low comprehensive utilization rate due to the problems of rough taste, difficult use and the like of the wheat bran; the wheat bran has the characteristics of high water retention, oil retention, adsorbability and the like, and biological matrixes such as the bran and the like can be mutually adsorbed with microorganisms to protect and improve the activity of the microorganisms; the bran is used for adsorbing the microbial agent, so that the treatment efficiency of the feces can be improved, the economic benefit is good, the resource utilization of the waste can be realized, and the circular economy of treating the waste with the waste is realized.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The strains used in the invention are all purchased from the market.
Examples
Preparing yeast, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis;
(1) respectively cultivating and propagating mother bacteria, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis to a high-density state through rejuvenation, wherein OD600 is more than 1;
(2) and (2) mixing the microorganisms in the step (1) according to the mass ratio of 2:3:1:1:1.5:1:2.5:2 to form the efficient microorganism composite microbial inoculum for decomposing and treating the feces.
When in use, wheat bran is treated by an ultrafine grinder to prepare fine bran, the fine bran is mixed with the efficient microbial compound inoculant, then the mixture is shaken in a shaking constant-temperature incubator for 2 to 4 days, and solid components in the mixture are separated by standing or centrifugation to obtain a solidified microbial compound inoculant; adding 50 ml of nutrient substance mixed solution of industrial brown sugar, antibiotics and amino acid solution required by microbial flora according to the mass ratio of 500:1:1 into every 200 g of solidified microbial composite inoculant; the solidified microbial composite inoculant is added with a filler carrier substance and then placed in a feces box of an ecological toilet for biological treatment of the toilet. Tests show that the high-efficiency microbial compound bacteria agent prepared by the method can keep activity at 70 ℃, and harmful bacteria such as escherichia coli, parasitic ova and the like and miscellaneous bacteria in excrement and urine can be killed at the high temperature of more than 60 ℃, so that safety, sanitation and harmlessness are further realized.
Claims (9)
1. The efficient microbial composite microbial inoculum for decomposing and treating feces is characterized by comprising saccharomycetes, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis.
2. The efficient composite microbial inoculant for decomposing and treating feces according to claim 1, wherein the mass ratio of the microbes is saccharomycetes, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis is 2:3:1:1:1.5:1:2.5: 2.
3. The method for preparing the efficient composite microbial inoculant for decomposing and treating feces according to claim 1 or 2, wherein the method comprises the following steps:
(1) respectively breeding mother bacteria, lactobacillus, bacillus subtilis, aspergillus niger, aspergillus oryzae, rhizopus and bacillus licheniformis which are screened from nature to a high-density state by rejuvenation, wherein OD600 is more than 1;
(2) and (2) mixing the microorganisms in the step (1) according to the mass ratio of 2:3:1:1:1.5:1:2.5:2 to form the target compound microbial agent.
4. The method for preparing solidified composite microbial inoculum of high-efficiency composite microbial inoculum for decomposing and treating feces according to claim 1 or 2, which is characterized by comprising the following steps:
processing wheat bran by an ultrafine grinder to prepare fine bran, mixing the fine bran with the efficient microbial compound inoculum for decomposing and processing the feces, then oscillating in an oscillating constant-temperature incubator for 2-4 days, and standing or centrifugally separating solid components to obtain the solidified microbial compound inoculum.
5. The method for preparing solidified microbial composite inoculum of high-efficiency microbial composite inoculum for decomposing and treating feces according to claim 4, wherein 50 ml of nutrient substance mixed liquor required by microbial flora is added in every 200 g of solidified microbial composite inoculum.
6. The method for preparing the solidified microbial composite inoculum of the high-efficiency microbial composite inoculum for decomposing and treating the feces according to claim 5, wherein the method for preparing the nutrient substance mixed solution is to mix industrial brown sugar, antibiotics and amino acid solution according to the mass ratio of 500:1: 1.
7. The method for preparing solidified composite microbial inoculum of high-efficiency composite microbial inoculum for decomposing and treating feces according to claim 4, wherein a filler carrier is added during use.
8. The method for preparing solidified composite microbial inoculum of high-efficiency composite microbial inoculum for decomposing and treating feces according to claim 7, wherein the filler carrier is a substance with strong water absorption.
9. The method for preparing solidified composite microbial inoculum of high efficiency composite microbial inoculum for decomposing and treating feces according to claim 8, wherein the substances with stronger water absorption are sawdust and wood chips.
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