CN104073445A - Microbial agent for pretreatment of straws and manure biogas - Google Patents

Microbial agent for pretreatment of straws and manure biogas Download PDF

Info

Publication number
CN104073445A
CN104073445A CN201410257058.4A CN201410257058A CN104073445A CN 104073445 A CN104073445 A CN 104073445A CN 201410257058 A CN201410257058 A CN 201410257058A CN 104073445 A CN104073445 A CN 104073445A
Authority
CN
China
Prior art keywords
stalk
cfu
ight soil
microbiobacterial agent
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410257058.4A
Other languages
Chinese (zh)
Other versions
CN104073445B (en
Inventor
乔维川
张瑞
张晓玉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Yuanyi Environment Biological Engineering Co Ltd
Original Assignee
Nanjing Yuanyi Environment Biological Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Yuanyi Environment Biological Engineering Co Ltd filed Critical Nanjing Yuanyi Environment Biological Engineering Co Ltd
Priority to CN201410257058.4A priority Critical patent/CN104073445B/en
Publication of CN104073445A publication Critical patent/CN104073445A/en
Application granted granted Critical
Publication of CN104073445B publication Critical patent/CN104073445B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/20—Sludge processing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a microbial agent for pretreatment of straws and manure biogas. The microbial agent jointly comprises the following strains: 2-8*10<10>cfu/g of Penicillium glaucum, 2-8*10<10>cfu/g of Rhizopus oryzae, 2-8*10<10>cfu/g of Phanerochaete chrysosporium, 2-8*10<10>cfu/g of Aspergillus flavus and 2-8*10<10>cfu/g of Spirillospora. By virtue of the microbial agent, the mixed anaerobic fermentation process of straws and manure can be promoted, the fermentation period is shortened, the biodegradation of organic materials can be accelerated, and a preparation method of the microbial agent is simple and easily implemented, the content of flora in feedstock per unit is large and the activity is high. The microbial agent can be used for the fermentation of different manure and straw raw materials in biogas fermentation enterprises and thus the harmless, reduction and resource utilization of manure and straws are achieved.

Description

A kind of stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent
Technical field
The invention belongs to agricultural waste fermentation pre-treatment microbial inoculum technical field, be specifically related to a kind of stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent.
Background technology
At present, approximately 700,000,000 tons of the stalks of the annual output of China, approximately 17.3 hundred million tons of livestock and poultry breeding industry ight soil quantity dischargeds.A large amount of agricultural wastes can not get rational utilization, are mostly directly burned or are discarded in field, river, so not only caused the waste of resource but also ecotope has been caused to serious destruction.Research shows, is about 0.45m taking stalk as fermenting raw materials production potential 3/ (KgTs), suitable with manure fermentation rate, its production potential is very huge.Produce biogas taking stalk as fermenting raw materials, both solved the problem of rural energy deficiency, opened up a new approach for solving " crop straw burning " problem again.Simultaneously the natural pond slag after biogas fermentation can production high-quality biogas residue organic fertilizer, for improving soil physical and chemical property, increases soil fertility, and can reduce the use of chemical fertilizer, promotes ecological agriculture construction.But because the main component in stalk is Mierocrystalline cellulose, hemicellulose and xylogen, if by direct stalk pit entry fermentation, the not destroyed and easy floating crust of the wax layer on stalk surface, causes aerogenesis not good.Therefore needed it to carry out pre-treatment before stalk enters pond.In recent years, correlative study report about degrading straw is more, but mostly concentrate on the research of physics, chemical process and enzyme solution etc., the research of screening, qualification and combination of deeply carrying out straw degradative bacterium is less, and the conventional straw degradative microbial inoculum in market mostly is the composite fungus agent that is applicable to field at present, but not the special bacteria agent of stalk marsh gas Biological Pretreatment.Stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent also face following problem at present:
(1) source of microorganism is extensive not enough.
Microbe species is various, distributed more widely, almost ubiquitous on earth.The place that germs collect is maximum is soil, and soil is the base camp of various microorganism growth breedings, gets arbitrarily handful of soil or a soil, be exactly a microbial world 1, no matter quantity or kind are all a lot.In fertile soil, every gram of soil contains 2,000,000,000 microorganisms, even dead soil also contains hundred million microorganisms of 3-5 in every gram of soil.Up to now, the microorganism known to us approximately has 100,000 kinds, but has only developed and found 1% of microbial species number in human being's production and life.When laboratory gathers bacterial classification sample, the principle of following is that the source of material is more extensive, more likely obtains new bacterial classification.Therefore, the efficient biogas fermentation pre-treatment of development of new microbial inoculum, must extensively draw materials, and sets certain specific environmental factors in laboratory, as characteristic of temperature, selected materials etc., so just can filter out microbial strains a large amount of and that contribute to fermenting process.
(2) composition of microbiobacterial agent is simple
At present microbiobacterial agent is taking screening simple function bacterium as main, or difference in functionality bacterium carried out simply composite.There is larger problem in this mode: first these single bacterium really have certain function in the time of laboratory culture, but at the complex condition fermenting, be but difficult to reach ideal effect.This be due to many single fungus strains can not be in actual environment growth and breeding or be easily limited by other microbial growths; Secondly,, owing to existing many kinds to interact between different microbial populations, the single bacterium of certain function that what laboratory screening got have can not fully prove that it has identical effect in a complicated colony; The 3rd, some microorganism biogas fermentation microbial inoculum, just fermentation strain is simply mixed, not thorough to the antagonistic action research between bacterial strain, this has just caused microbial strains during the fermentation because the antagonistic action between bacterial strain does not reach desired effect; The 4th, can not effectively utilize the effect of the various enzymes of microorganisms.In organic degradation process, the Degradation of enzyme is very crucial, therefore, only contains microorganism, and the microbiobacterial agent that does not contain enzyme can not be brought into play larger effect.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, the object of this invention is to provide a kind of stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent, promote stalk ight soil mixing anaerobic biogas fermentation process, shorten fermentation period, accelerate the organic biological degradation of raw material.For the fermentation of different ight soil and stalk, make that ight soil and stalk are innoxious, minimizing, resource utilization.Another object of the present invention is to provide the application of mentioned microorganism microbial inoculum.
Technical scheme: in order to realize foregoing invention object, the technical solution used in the present invention is as follows:
A kind of stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent, be made up of jointly following bacterial classification: Penicillum glaucum ( penicillium glaucum) 2 ~ 8 × 10 10cfu/g, Rhizopus oryzae ( rhizopus oryzae) 2 ~ 8 × 10 10cfu/g, Phanerochaete chrysosporium ( phanerochaete chrysosporium) 2 ~ 8 × 10 10cfu/g, flavus ( aspergillus flavus) 2 ~ 8 × 10 10cfu/g, spiral shell spore bacterium ( spirillospora) 2 ~ 8 × 10 10cfu/g.
A fermentation process for stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent, specific as follows:
1) glucose is dissolved in water, then adds microbial inoculum fully to stir evenly, microbial inoculum is melted, then diluent is evenly splashed on stalk, and fully stir evenly;
2) stalk after treatment step 1) is dropped in fermentor tank, add ight soil, carry out the producing methane through anaerobic fermentation of stalk and ight soil;
Wherein, the part by weight of material composition: ight soil 20% ~ 25%, stalk 1% ~ 2%, glucose 0.01% ~ 0.02%, microbiobacterial agent 0.01% ~ 0.02%, tap water 70% ~ 80%; Material total moisture should be controlled at 90 ~ 92%, pH and be neutrality or weakly alkaline, and material particular diameter is at 5 ~ 20mm.
By regulate the pH value of material with soda-lime or acetic acid.
Leavening temperature is 37 DEG C, and stir 1-2 every day.
The mass ratio of microbiobacterial agent and stalk is 1:50.
Beneficial effect: compared with prior art, stalk of the present invention and ight soil biogas fermentation pre-treatment microbiobacterial agent, can promote stalk ight soil hybrid anaerobic fermentation process, shorten fermentation period, can accelerate the organic biological degradation of raw material, and the making method of microbial inoculum is simple, easy to implement, in unit raw material, micro-flora content is large, active high.Can be applicable to the fermentation of biogas fermentation enterprise for different ight soil and stalk, thereby make ight soil and stalk is innoxious, minimizing, resource utilization.There are following performance index:
(1) product appearance and the quality guaranteed period: product is that khaki color is Powdered, odorless, shady and cool (20 DEG C are following) dry place preserves, validity period 1 year.
(2) use fermentation maturity phase of microbial inoculum: stalk (mixing with ight soil) is 10-16 days.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
Mainly various cellulose-degrading bacterias, the lignin-degrading bacteria from fermentation biogas liquid of microorganism in stalk and ight soil biogas fermentation pre-treatment microbiobacterial agent, screenings such as various bacteriums in ripe compost, trees soil, actinomycetes, fungi and obtaining.
(1) isolation and screening of microorganism
The isolation and screening of cellulose-degrading bacteria, lignin-degrading bacteria, it is microbe-derived getting the existing marsh-gas fermentation tank in laboratory, make sole carbon source with stalk powder, preparation minimal medium, adopt dilution-plate method to separate different microorganisms, 32 DEG C of cultivations, isolate each 12 strains of aerobic and double type anerobe.Then, stalk is carried out to pre-treatment with every kind of microorganism, by measuring its overall degradation rate to Mierocrystalline cellulose, hemicellulose, xylogen, filter out higher six kinds of effect, be numbered h4, h10, h13, y2, y7 and y11.
The isolation and screening of various bacteriums in ripe compost, trees soil, actinomycetes, fungi, getting compost and soil is bacterial classification source, adopt dilution-plate method to separate different microorganisms, wherein substratum adopts respectively beef-protein medium, Gause I substratum, Cha Shi substratum and PDA substratum to isolate respectively bacterium, actinomycetes, mould and fungi.32 DEG C of cultivations, and measure by increment, filter out respectively the microorganism of increment maximum, and number consecutively is n4, n6, g1, g8, zd5, zd10, zt4, m2, m9 and m10.
(2) microorganism is sieved again
Above-mentioned 16 kinds of microbial inoculants that filter out are grown in the liquid substratum of the inorganic salt of making sole carbon source with stalk powder, then measure cellulase activity and the laccase activity of bacterium liquid.By the size of living of enzyme relatively, filter out larger 12 kinds of Enzyme activity: h4, h10, h13, y2, y11, m9, m10, zd5, zd10, zt4, g1 and g8.
(3) antagonistic experiment between different microorganisms
Above-mentioned 12 kinds of microorganisms that filter out are inoculated in agar nutrition flat board between two, 30 DEG C of illumination cultivation, through the cultivation of three days, observe the growing state of different microorganisms, wherein there is microorganism to grow if found, illustrate that its growth is subject to the inhibition of other microorganisms, can filter out thus competitive strong microorganism.Experimental result discovery, competitive strong microorganism is wherein 10 kinds: h4, h10, h13, y2, y11, m9, m10, zd10, g1 and g8.
(4) bacterial classification Experiment of Compatibility
Above-mentioned 10 kinds of microorganisms that filter out are carried out to compatibility, have 12 groups (as table 1).
Table 1 bacterial strain compatibility
These 12 groups of microorganisms are inoculated into respectively to the mixed-culture medium (mass ratio of basic medium and wheat bran, substratum: wheat bran=10:1) in, carrying out common fermentation enlarged culturing, culture condition is shaking table shaking culture, 30 DEG C of illumination, then measure cellulase activity and the laccase activity of bacterium liquid.By relatively enzyme is alive big or small, filter out 4 groups of microorganism compatibilities that Enzyme activity is larger.Again nutrient solution is carried out to centrifugation (5000r/min rotating speed), obtain microbial cells, finally vacuum-drying 5h at 60 DEG C, obtain 4 kinds of microbiobacterial agents, it is respectively No. 2 microbial inoculums (containing bacterium m10, g8, h13, g1 and y2), No. 6 microbial inoculums (containing bacterium y11, h13, zd10, h10 and g1), No. 8 microbial inoculums (containing bacterium m10, h8, zd10 and h10) and No. 12 microbial inoculums (containing bacterium m10, m9, h4, h13 and h10).
Product is that khaki color is Powdered, odorless, and shady and cool (20 DEG C are following) dry place preserves, validity period 1 year.
Embodiment 2
4 kinds of microbial bacterias prepared by Application Example 1 carry out pre-treatment to stalk, more pretreated stalk is dropped in fermentor tank and carries out anaerobically fermenting, filter out the one that gas production rate is the highest.Specific as follows:
1) weight of material ratio composition
(wetting) ight soil 20% ~ 25%, stalk 1% ~ 2%, glucose 0.01% ~ 0.02%, microbiobacterial agent 0.01% ~ 0.02%, tap water 70% ~ 80%.
2) making processes
(1) reactivation process of microbial inoculum
First glucose is dissolved in water, then adds microbial inoculum fully to stir evenly, microbial inoculum is melted, then diluent is evenly splashed on stalk, and fully stir (with stirrer) evenly.
(2) fermenting process
Pretreated stalk is dropped in fermentor tank and carries out anaerobically fermenting.Taking the identical material that do not add microbiobacterial agent as contrast, carry out stalk and swine waste marsh gas fermentation, the size of contrast gas production rate every day.
Material moisture should be controlled at 90 ~ 92%, pH and be neutrality or weakly alkaline, and material particular diameter is at 5 ~ 20mm.When anaerobically fermenting, microbial inoculum, the stalk of smashing, pig manure and tap water will fully be uniformly mixed.The end of collecting methane pipe is immersed in water.Fermentor tank is placed in 37 DEG C of thermostats, and from fermentation, stir 1-2 every day certainly.
By regulate the pH value of material with soda-lime or acetic acid.
Gas production rate size is shown in Fig. 1, visible stalk of the present invention and ight soil biogas fermentation pre-treatment microbiobacterial agent (No. 6 microbial inoculums) can promote stalk ight soil hybrid anaerobic fermentation process, shorten fermentation period (10-16 days), can accelerate the organic biological degradation of raw material, and the making method of microbial inoculum is simple, easy to implement, in unit raw material, micro-flora content is large, active high.Can be applicable to the fermentation of biogas fermentation enterprise for different ight soil and stalk, thereby make ight soil and stalk is innoxious, minimizing, resource utilization.
Finally, 5 kinds of microorganisms in No. 6 microorganism compatibilities are identified, it is respectively: Penicillum glaucum ( penicillium glaucum) 2 ~ 8 × 10 10cfu/g, Rhizopus oryzae ( rhizopus oryzae) 2 ~ 8 × 10 10cfu/g, Phanerochaete chrysosporium ( phanerochaete chrysosporium) 2 ~ 8 × 10 10cfu/g, flavus ( aspergillus flavus) 2 ~ 8 × 10 10cfu/g, spiral shell spore bacterium ( spirillospora) 2 ~ 8 × 10 10cfu/g.Be common bacterial classification.
Embodiment 3
The advantage microbial inoculum (No. 6 microorganisms of embodiment 2) filtering out according to gas production rate size, mixes with stalk by different ratios, carries out pre-treatment, puts into 37 DEG C of constant incubators, anaerobically fermenting.By the size of biogas amount, finally determine the optimum charging ratio (mass ratio) of microbial inoculum and stalk.
The mass ratio of dry bacteria and stalk is got respectively No. 1 1:1000, No. 2 1:500, No. 3 1:100, No. 4 1:50 and No. 5 1:20, wherein the unified 15g that adds of stalk.
Experiment shows: when the mass ratio of dry bacteria and stalk is 1:50, gas production rate is the highest.Therefore, selecting 1:50 is optimum charging ratio.

Claims (5)

1. stalk and an ight soil biogas fermentation pre-treatment microbiobacterial agent, is characterized in that, is jointly made up of: Penicillum glaucum 2 ~ 8 × 10 following bacterial classification 10cfu/g, Rhizopus oryzae 2 ~ 8 × 10 10cfu/g, Phanerochaete chrysosporium 2 ~ 8 × 10 10cfu/g, flavus 2 ~ 8 × 10 10cfu/g, spiral shell spore bacterium 2 ~ 8 × 10 10cfu/g.
2. the fermentation process of stalk claimed in claim 1 and ight soil biogas fermentation pre-treatment microbiobacterial agent, its feature is specific as follows:
1) glucose is dissolved in water, then adds microbial inoculum fully to stir evenly, microbial inoculum is melted, then diluent is evenly splashed on stalk, and fully stir evenly;
2) stalk after treatment step 1) is dropped in fermentor tank, add ight soil, carry out the producing methane through anaerobic fermentation of stalk and ight soil;
Wherein, the part by weight of material composition: ight soil 20% ~ 25%, stalk 1% ~ 2%, glucose 0.01% ~ 0.02%, microbiobacterial agent 0.01% ~ 0.02%, tap water 70% ~ 80%; Material total moisture should be controlled at 90 ~ 92%, pH and be neutrality or weakly alkaline, and material particular diameter is at 5 ~ 20mm.
3. the compost method of stalk according to claim 2 and ight soil hybrid composting microbiobacterial agent, is characterized in that: by regulate the pH value of material with soda-lime or acetic acid.
4. the compost method of stalk according to claim 2 and ight soil hybrid composting microbiobacterial agent, is characterized in that: leavening temperature is 37 DEG C, and stir 1-2 every day.
5. the compost method of stalk according to claim 2 and ight soil hybrid composting microbiobacterial agent, is characterized in that: the mass ratio of microbiobacterial agent and stalk is 1:50.
CN201410257058.4A 2014-06-11 2014-06-11 A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation Expired - Fee Related CN104073445B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410257058.4A CN104073445B (en) 2014-06-11 2014-06-11 A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410257058.4A CN104073445B (en) 2014-06-11 2014-06-11 A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation

Publications (2)

Publication Number Publication Date
CN104073445A true CN104073445A (en) 2014-10-01
CN104073445B CN104073445B (en) 2016-08-24

Family

ID=51595052

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410257058.4A Expired - Fee Related CN104073445B (en) 2014-06-11 2014-06-11 A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation

Country Status (1)

Country Link
CN (1) CN104073445B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105543287A (en) * 2016-02-24 2016-05-04 齐芳 Treatment method for preparing methane from brewing wastewater
CN109328514A (en) * 2018-10-23 2019-02-15 湖南杂交水稻研究中心 A kind of saline and alkali land improvement method and its application
CN110002706A (en) * 2019-04-23 2019-07-12 上海舒圣投资管理有限公司 A kind of pig farm excreta method for innocent treatment
CN113088464A (en) * 2021-03-26 2021-07-09 张掖兰标生物科技有限公司 Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102008011A (en) * 2010-11-16 2011-04-13 马德顺 Straw degradation process
CN102173507A (en) * 2011-01-12 2011-09-07 南京加德绿色能源研发有限公司 Method for comprehensively recycling fecal sewage of large-scale livestock and poultry farms and farmland crop straws
CN103232944A (en) * 2013-04-25 2013-08-07 南京林业大学 Microorganism bacterium agent for straw and excrement mixed composting

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102008011A (en) * 2010-11-16 2011-04-13 马德顺 Straw degradation process
CN102173507A (en) * 2011-01-12 2011-09-07 南京加德绿色能源研发有限公司 Method for comprehensively recycling fecal sewage of large-scale livestock and poultry farms and farmland crop straws
CN103232944A (en) * 2013-04-25 2013-08-07 南京林业大学 Microorganism bacterium agent for straw and excrement mixed composting

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张瑞 等: "秸秆沼气发酵预处理微生物菌剂的筛选与特征研究", 《安徽农业科学》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105543287A (en) * 2016-02-24 2016-05-04 齐芳 Treatment method for preparing methane from brewing wastewater
CN109328514A (en) * 2018-10-23 2019-02-15 湖南杂交水稻研究中心 A kind of saline and alkali land improvement method and its application
CN109328514B (en) * 2018-10-23 2021-11-30 湖南杂交水稻研究中心 Saline-alkali soil improvement method and application thereof
CN110002706A (en) * 2019-04-23 2019-07-12 上海舒圣投资管理有限公司 A kind of pig farm excreta method for innocent treatment
CN110002706B (en) * 2019-04-23 2022-08-16 上海舒圣投资管理有限公司 Harmless treatment method for excrement of pig farm
CN113088464A (en) * 2021-03-26 2021-07-09 张掖兰标生物科技有限公司 Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof

Also Published As

Publication number Publication date
CN104073445B (en) 2016-08-24

Similar Documents

Publication Publication Date Title
CN103232944B (en) Microorganism bacterium agent for straw and excrement mixed composting
CN110066746B (en) High-temperature-resistant bacillus bacterium NJAU-ND8 for accelerating compost maturity and application thereof
CN103011916B (en) Method for preparing organic bacteria liquid, organic bacteria liquid prepared by method and application of organic bacteria liquid
CN101775359A (en) Special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof
CN105524858B (en) A kind of high temperature resistant decomposing microbial inoculum of organic waste and its application
CN105936881B (en) One kind is for alignic thermophilic sugared bacillus and its application method of degrading
CN102442849A (en) Disease-preventing bio-organic fertilizer prepared from straw and its preparation method
CN109880771B (en) One plant of bacillus thermophilic bacteria NJAU-N30 for accelerating compost maturity and its application
CN111363684B (en) Composite microbial inoculum for efficiently degrading wood fibers and application thereof in composting
CN103484410B (en) Biological agent for compost treatment of livestock manure
CN106350469A (en) Bacillus with high temperature resistance and cellulose degradation capacity and application thereof
CN103087973A (en) Screening and domesticating method of composite microbial system for low-temperature degradation of straws
CN111073839B (en) Siam bacillus, microbial inoculum and application thereof
CN103396182B (en) Method for producing solanaceous vegetable seedling growing substrate by utilizing salvaged material of cassava processing
CN104177138B (en) Biological bacterial fertilizer prepared through solid fermentation technology and application of biological bacterial fertilizer
CN101864363B (en) Complex bacterial preparation and application thereof
CN104073445B (en) A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation
CN101519644B (en) Sinorhizobium sp. and application thereof
CN108865927B (en) Bacterial strain for low-temperature glycolysis of corn straw and fermentation culture method and application thereof
CN110819555B (en) High-temperature-resistant Brevibacillus reuteri NJAU-N20 for efficiently degrading wood fibers and application thereof
CN101993832A (en) Composite bacterial agent for pig manure compost treatment and application method
CN110622825A (en) Tobacco seedling culture medium and preparation method and application thereof
CN108410772A (en) A kind of preparation method of straw biological decomposing agent
CN113481111A (en) Efficient biological straw fermentation inoculant and preparation method thereof
CN106754420A (en) Using the method for blue-green algae mud culture Trichoderma

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160824

Termination date: 20180611