CN106754420A - Using the method for blue-green algae mud culture Trichoderma - Google Patents
Using the method for blue-green algae mud culture Trichoderma Download PDFInfo
- Publication number
- CN106754420A CN106754420A CN201710012606.0A CN201710012606A CN106754420A CN 106754420 A CN106754420 A CN 106754420A CN 201710012606 A CN201710012606 A CN 201710012606A CN 106754420 A CN106754420 A CN 106754420A
- Authority
- CN
- China
- Prior art keywords
- blue
- green algae
- algae mud
- trichoderma
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of method of utilization blue-green algae mud culture Trichoderma, comprise the following steps：Broken wall treatment is carried out after blue-green algae mud is redissolved, neutral proteinase is subsequently adding, 2 4h are hydrolyzed at 40 50 DEG C, add alkali protease, 2 5h are hydrolyzed at 50 55 DEG C, obtain blue-green algae mud hydrolyzate；Blue-green algae mud hydrolyzate is configured to fermentation medium, Trichoderma is inoculated into fermentation medium, carry out fermented and cultured.The present invention carries out recycling with blue-green algae mud as raw material to blue-green algae mud, and the blue-green algae hydrolyzate rich in small peptide/amino acid is obtained after Protease Treatment, is made the Trichoderma cultivated after culture medium, is used directly for agricultural production.
Description
Technical field
The present invention relates to resource regeneration and technical field of bioengineering, more particularly to one kind is using blue-green algae mud culture wood
The method of mould.
Background technology
Blue-green algae is a class Gram-negative, atrichia, containing chlorophyll a, do not form chloroplaset, can carry out producing oxygen light cooperation
Prokaryotes.In recent years, frequently, a large amount of blue-green algaes are salvaged up the ground such as Taihu Lake, Dian Chi, Chaohu blue algae bloom, expend big
Amount manpower and financial resources.But the blue-green algae that gets is aqueous to reach more than 98%, it is impossible to preserve, if not in time place comprehend it is smelly,
And then cause secondary pollution.
Processing method common to blue-green algae mud at present includes：(1) compost treatment：Degradable Algae toxins, but peasant does not like
It is joyous to use, and when using with the seasonal mismatch of crops；(2) fermentation methane production：Can together with the rubbish such as pig manure fermentation methane,
But due to carbon containing low, produce that methane quantity is few, biogas residue can produce secondary pollution；(3) drying and processing supply further with：It has been used as
The report of degradation plastic raw material, but drying cost is higher, makes that its stink difficulty after plastics is removed and selling price is not high, it is difficult to support
Disappear salvaging drying cost；(4) phycobniliprotein, Algae toxins, pigment, polysaccharide etc. are extracted, but purifying cost is very high；(5) it is used as mushroom
Mushroom cultivates dispensing or foster yeast single cell protein culture, but Algae toxins cannot be removed, and added value is not high；(6) Su Yun is cultivated
Golden bacillus produces biological insecticides, belongs to direct fermentation blue-green algae mud, and blue-green algae nutritional utilization is not abundant enough.
Blue-green alga bloom outburst needs to pick up blue-green algae from lake rapidly with sudden mainly in annual 5~September, during treatment.
But blue-green algae amount is too big, if cannot all process immediately, because blue-green algae is rich in nutrition (up to more than 40% protein content), meeting
Generation is corrupt to trigger stench and secondary pollution.In order to effectively utilize blue-green algae, drying technology is successfully developed at present, blue-green algae is dried
Blue-green algae mud/powder that moisture is about 5~30% is formed, such blue-green algae would not be smelly, and volume is substantially reduced, be conducive to the later stage blue
Algae utilizes.But, the technology for carrying out high value added product exploitation currently for blue-green algae mud/powder is also immature.For example, patent
The Chinese patent of Application No. 201110430308.6 is that raw material production eats White mushroom with blue-green algae and straw, but blue-green algae is necessary
Addition pseudomonas aeruginosa and Sphingol single-cell carry out aerobic fermentation treatment to remove cyanophycean toxin.Number of patent application is
201510865971.7 Chinese patent is directly used to cultivate thuringiensis as life using fresh blue alga biogas slurry as raw material
Thing insecticide, but the technology is relatively difficult for processing the bloom blue algae of irregular outburst.In addition, Chinese patent
201610320145.9 disclose blue algae mud and stalk, calcareous fertilisers etc. are carried out according to a certain percentage compost fermentation be made it is organic
Fertilizer, then it is mixed and made into the technology of composite microbiological fertilizer according to a certain percentage with urea, ammonium chloride, potassium chloride, attapulgite.And
In technology disclosed above, the recycling treatment process value-added content of product of bloom blue algae is not high.
Trichoderma belongs to Fungi Imperfecti door, and hyphomycetales, trichoderma, common trichoderma has Trichoderma viride, trichodermaharzianum, Trichoderma harzianum
Deng.Trichoderma has number of mechanisms to the antagonism of phytopathogen, it is considered that its mechanism includes competition, hyperparasite, association
Same antagonism, antibiosis, induction of resistance etc..The Trichoderma such as Trichoderma viride, Trichoderma harzianum approved is used as agriculture biocontrol microorganisms, the wood of production
Mould agent is mainly conidium agent；It is more next in green agriculture production because Trichoderma is environmentally safe friendly bacterium
More favored.Trichoderma contains the enzyme systems such as abundant protease, amylase, and such as Lee filters out one plant has powerful starch water
The trichoderma J113 of solution ability, can be used to hydrolyze spirulina polysaccharide (Bioenerg.Res. (2015) 8:1868–1876).But, mesh
The culture of preceding Bio-control Trichoderma relies primarily on grain raw material, such as corn, potato, and its toxigenic capacity is higher.
The content of the invention
To solve the above problems, it is an object of the invention to provide a kind of method of utilization blue-green algae mud culture Trichoderma, this hair
It is bright that recycling is carried out to blue-green algae mud with blue-green algae mud as raw material, the indigo plant rich in small peptide/amino acid is obtained after Protease Treatment
Algae hydrolyzate, is made the Trichoderma cultivated after culture medium, can be directly used as the biocontrol microorganisms of agricultural production, prevents plant disease.
The invention provides a kind of method of utilization blue-green algae mud culture Trichoderma, comprise the following steps：
(1) blue-green algae mud and water are pressed into 5-10:Broken wall treatment is carried out after 1 redissolution, neutral proteinase is subsequently adding, in 40-50
2-4h is hydrolyzed at DEG C, alkali protease is added, 2-5h is hydrolyzed at 50-55 DEG C, obtain blue-green algae mud hydrolyzate；
(2) the blue-green algae mud hydrolyzate for obtaining step (1) is configured to fermentation medium, and Trichoderma is inoculated into fermented and cultured
In base, fermented and cultured is carried out.
Further, in step (1), blue-green algae be Microcystis aeruginosa, nostoc, anabena, sheath silk algae and Closterium in one
Plant or several.Blue-green algae is the blue-green algae mud for salvaging dehydration, and water content is 5-30%.
Further, in step (1), neutral proteinase is the 1-3% of blue-green algae shale amount.
Further, in step (1), alkali protease is the 2-5% of blue-green algae shale amount.
Further, in step (2), Trichoderma be Trichoderma harzianum GIM 3.442, Trichoderma viride CGMCC 3.2942 or
Trichodermaharzianum.
Further, in step (2), fermentation medium is solid-state fermentation culture medium or liquid fermentation medium.
Further, solid-state fermentation culture medium includes each component of following content：10-60 parts of blue-green algae mud hydrolyzate, stalk
Powder 10-30 parts, wheat bran 5-20 parts, glucose 5-15 parts, potassium dihydrogen phosphate 3-10 parts, magnesium sulfate 1.5-5 parts.Blue-green algae mud hydrolyzate
Middle blue-green algae mud is 5-10 with the mass ratio of water:1.
Further, solid-state fermentation culture medium includes each component of following content：50-55 parts of blue-green algae mud hydrolyzate, stalk
Powder 25-30 parts, wheat bran 5-10 parts, glucose 5-10 parts, potassium dihydrogen phosphate 3-4 parts, magnesium sulfate 1.5-2 parts.Blue-green algae mud hydrolyzate
Middle blue-green algae mud is 5-10 with the mass ratio of water:1.
Further, every liter of liquid fermentation medium includes each component of following content：Blue-green algae mud hydrolyzate 25-50g, Portugal
Grape sugar 5-10g, ammonium sulfate or sodium nitrate 1.0-3.0g, potassium dihydrogen phosphate 4.0-6.0g, magnesium sulfate 0.5-2.0g, calcium chloride 0.5-
1.5g, balance of water.Blue-green algae mud and the mass ratio of water are 5-10 in blue-green algae mud hydrolyzate:1.
Further, every liter of liquid fermentation medium includes each component of following quality：Blue-green algae mud hydrolyzate 20-40g, Portugal
Grape sugar 4-18g, ammonium sulfate or sodium nitrate 1.0-3.0g, potassium dihydrogen phosphate 3.0-10.0g, magnesium sulfate 1.0-2.0g, calcium chloride
0.3-1.0g, balance of water.Blue-green algae mud and the mass ratio of water are 5-10 in blue-green algae mud hydrolyzate:1.
Further, in step (2), ventilated culture Trichoderma 5-7 using solid-state fermentation culture medium at 25-28 DEG C
My god.
Further, in step (2), using liquid fermentation medium at 25-30 DEG C, training of being ventilated under pH 6.0-7.0
Support Trichoderma 3-5 days.
By such scheme, the present invention at least has advantages below：
The present invention carries out recycling for bloom blue algae is salvaged, and makes full use of in blue-green algae containing a large amount of albumen (40-
65%), the nutritional characteristic such as polysaccharide (5-10%), after being digested to blue-green algae, blue-green algae mud hydrolyzate is rich in small peptide and amino acid, then
Add other nutrients and be directly made into culture medium, Trichoderma is obtained by solid state fermentation or submerged fermentation, can be used as agriculture life
It is fungi-proofing, crops resistance disease ability is improved, green agriculture development is also beneficial to, promote agricultural sustainable development.
Described above is only the general introduction of technical solution of the present invention, in order to better understand technological means of the invention,
And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate detailed description as after.
Specific embodiment
With reference to embodiment, specific embodiment of the invention is described in further detail.Following examples are used for
The present invention is illustrated, but is not limited to the scope of the present invention.
The Trichoderma that the present invention is used includes：
Trichoderma harzianum GIM 3.442, is purchased from Guangdong province, China's Culture Collection, with PDA culture medium culture and
Preservation；Trichoderma viride CGMCC 3.2942, is purchased from China General Microbiological DSMZ, with PDA culture medium culture and guarantor
Hide.
The preparation of the blue-green algae mud hydrolyzate of embodiment 1
50g butt blue-green algae mud is taken, with water is in mass ratio 6 by it:1 is stirred redissolution, in homogenizer high speed broken wall
Treatment 10min, to neutral proteinase 1% (on the basis of butt blue-green algae mud) is added in liquid, 2h is hydrolyzed at 45 DEG C；Add alkali
Property protease 3 % (on the basis of butt blue-green algae mud), hydrolyzes 3h at 55 DEG C, obtains blue-green algae mud hydrolyzate, measures its albumen water
Xie Du is 65%.
The preparation of the blue-green algae mud hydrolyzate of embodiment 2
50g butt blue-green algae mud is taken, with water is in mass ratio 5 by it:1 is stirred redissolution, in homogenizer high speed broken wall
Treatment 10min, to neutral proteinase 3% (on the basis of butt blue-green algae mud) is added in liquid, 4h is hydrolyzed at 40 DEG C；Add alkali
Property protease 5% (on the basis of butt blue-green algae mud), hydrolyzes 2h at 55 DEG C, obtains blue-green algae mud hydrolyzate.
The preparation of the blue-green algae mud hydrolyzate of embodiment 3
50g butt blue-green algae mud is taken, with water is in mass ratio 10 by it:1 is stirred redissolution, in homogenizer high speed broken wall
Treatment 10min, to neutral proteinase 3% (on the basis of butt blue-green algae mud) is added in liquid, 4h is hydrolyzed at 50 DEG C；Add alkali
Property protease 2% (on the basis of butt blue-green algae mud), hydrolyzes 5h at 50 DEG C, obtains blue-green algae mud hydrolyzate.
The solid state fermentation culture of the Trichoderma harzianum of embodiment 4
To addition powder of straw 25g, wheat bran 10g, glucose 5g, biphosphate in the blue-green algae mud hydrolyzate that embodiment 1 is obtained
Potassium 3g, magnesium sulfate 1.5g, the water content in adjustment culture medium, by the mass percent (on the basis of butt blue-green algae mud) of blue-green algae mud
Control obtains solid-state fermentation culture medium the 30% of gross weight.
Trichoderma harzianum GIM 3.442 is inoculated into above-mentioned solid-state fermentation culture medium according to 8%, in tray solid-state fermentation installation
In 25 DEG C ventilation culture 7 days, interval stirred, final spore content to 1.5 × 109Individual/g.
The solid state fermentation culture of the Trichoderma viride of embodiment 5
To addition powder of straw 30g, wheat bran 5g, glucose 10g, biphosphate in the blue-green algae mud hydrolyzate that embodiment 1 is obtained
Potassium 4g, magnesium sulfate 2g, the water content in adjustment culture medium, the mass percent of blue-green algae mud is controlled 55%, obtains solid-state hair
Ferment culture medium.
Trichoderma viride CGMCC 3.2942 is inoculated into above-mentioned solid-state fermentation culture medium according to 8%, in tray solid state fermentation
26 DEG C of ventilations are cultivated 7 days in device, and interval is stirred, final spore content to 2.2 × 109Individual/g.
The Submerged fermentation of the Trichoderma harzianum of embodiment 6
The blue-green algae mud hydrolyzate prepared using embodiment 1, liquid fermentation medium is prepared according to following formula：Blue-green algae mud
30g/L, glucose 10g/L, ammonium sulfate 2.5g/L, potassium dihydrogen phosphate 6.0g/L, magnesium sulfate 0.8g/L, calcium chloride 0.5g/L, pH
6.0。
Take 4L aforesaid liquid culture mediums to be placed in 7L fermentation tanks, Trichoderma harzianum GIM3.442 is accessed with 5% inoculum concentration, logical
Gas 1vvm, speed of agitator 300rpm, cultivate 4 days under the conditions of 25 DEG C, final spore content is 2.6 × 108Individual/mL.
The Submerged fermentation of the Trichoderma viride of embodiment 7
The blue-green algae mud hydrolyzate prepared using embodiment 1, liquid fermentation medium is prepared according to following formula：Blue-green algae mud
25g/L, glucose 5.0g/L, sodium nitrate 2.0g/L, potassium dihydrogen phosphate 4.0g/L, magnesium sulfate 0.6g/L, calcium chloride 0.5g/L, pH
7.0。
Take 100mL aforesaid liquid culture mediums to be placed in 500mL shaking flasks, Trichoderma viride CGMCC is accessed with 8% inoculum concentration
3.2942, the shaken cultivation 5 days under the conditions of speed of agitator 200rpm, 26 DEG C, final spore content is 9.3 × 107Individual/mL.
The above is only the preferred embodiment of the present invention, is not intended to limit the invention, it is noted that for this skill
For the those of ordinary skill in art field, on the premise of the technology of the present invention principle is not departed from, can also make it is some improvement and
Modification, these are improved and modification also should be regarded as protection scope of the present invention.
Claims (12)
1. a kind of method of utilization blue-green algae mud culture Trichoderma, it is characterised in that comprise the following steps：
(1) broken wall treatment is carried out after blue-green algae mud is redissolved, neutral proteinase is subsequently adding, 2-4h is hydrolyzed at 40-50 DEG C, then add
Enter alkali protease, 2-5h is hydrolyzed at 50-55 DEG C, obtain blue-green algae mud hydrolyzate；
(2) the blue-green algae mud hydrolyzate that step (1) is obtained is configured to fermentation medium, Trichoderma is inoculated into fermented and cultured
In base, fermented and cultured is carried out.
2. the method for utilization blue-green algae mud culture Trichoderma according to claim 1, it is characterised in that：It is blue in step (1)
Algae be Microcystis aeruginosa, nostoc, anabena, sheath silk algae and Closterium in one or more.
3. the method for utilization blue-green algae mud culture Trichoderma according to claim 1, it is characterised in that：In step (1), institute
State the 1-3% that neutral proteinase is blue-green algae shale amount.
4. the method for utilization blue-green algae mud culture Trichoderma according to claim 1, it is characterised in that：In step (1), institute
State the 2-5% that alkali protease is blue-green algae shale amount.
5. the method for utilization blue-green algae mud culture Trichoderma according to claim 1, it is characterised in that：In step (2), institute
Trichoderma is stated for Trichoderma harzianum, Trichoderma viride or trichodermaharzianum.
6. the method for utilization blue-green algae mud culture Trichoderma according to claim 1, it is characterised in that：In step (2), institute
Fermentation medium is stated for solid-state fermentation culture medium or liquid fermentation medium.
7. the method for utilization blue-green algae mud culture Trichoderma according to claim 6, it is characterised in that the solid state fermentation training
Supporting base includes each component of following content：10-60 parts of blue-green algae mud hydrolyzate, powder of straw 10-30 parts, wheat bran 5-20 parts, glucose
5-15 parts, potassium dihydrogen phosphate 3-10 parts, magnesium sulfate 1.5-5 parts.
8. the method for utilization blue-green algae mud culture Trichoderma according to claim 7, it is characterised in that the solid state fermentation training
Supporting base includes each component of following content：50-55 parts of blue-green algae mud hydrolyzate, powder of straw 25-30 parts, wheat bran 5-10 parts, glucose
5-10 parts, potassium dihydrogen phosphate 3-4 parts, magnesium sulfate 1.5-2 parts.
9. the method for utilization blue-green algae mud culture Trichoderma according to claim 6, it is characterised in that every liter of liquid hair
Ferment culture medium includes each component of following content：Blue-green algae mud hydrolyzate 25-50g, glucose 5-10g, ammonium sulfate or sodium nitrate
1.0-3.0g, potassium dihydrogen phosphate 4.0-6.0g, magnesium sulfate 0.5-2.0g, calcium chloride 0.5-1.5g, balance of water.
10. the method for utilization blue-green algae mud culture Trichoderma according to claim 9, it is characterised in that every liter of liquid
Fermentation medium includes each component of following content：Blue-green algae mud hydrolyzate 20-40g, glucose 4-18g, ammonium sulfate or sodium nitrate
1.0-3.0g, potassium dihydrogen phosphate 3.0-10.0g, magnesium sulfate 1.0-2.0g, calcium chloride 0.3-1.0g, balance of water.
The method of 11. utilization blue-green algae mud culture Trichodermas according to claim 6, it is characterised in that：In step (2),
Ventilated at 25-28 DEG C using solid-state fermentation culture medium and cultivate the Trichoderma.
The method of 12. utilization blue-green algae mud culture Trichodermas according to claim 6, it is characterised in that：In step (2),
Ventilated using liquid fermentation medium at a temperature of 25-30 DEG C, under pH 6.0-7.0 and cultivate the Trichoderma.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710012606.0A CN106754420B (en) | 2017-01-09 | 2017-01-09 | Method for culturing trichoderma by using blue algae mud |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710012606.0A CN106754420B (en) | 2017-01-09 | 2017-01-09 | Method for culturing trichoderma by using blue algae mud |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106754420A true CN106754420A (en) | 2017-05-31 |
| CN106754420B CN106754420B (en) | 2021-02-09 |
Family
ID=58950324
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710012606.0A Active CN106754420B (en) | 2017-01-09 | 2017-01-09 | Method for culturing trichoderma by using blue algae mud |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106754420B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810929A (en) * | 2019-03-27 | 2019-05-28 | 江南大学 | A method of utilizing the clay standby active microbial inoculum of cyanobacteria |
| CN113862187A (en) * | 2021-10-14 | 2021-12-31 | 华南农业大学 | Method for producing agricultural microbial agent by treating pig farm biogas slurry through nitrogen-fixing blue algae and trichoderma combined and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106007913A (en) * | 2016-05-13 | 2016-10-12 | 安徽金农惠民生物技术有限公司 | Method for preparing compound microbial fertilizer with cyanophyta mud |
-
2017
- 2017-01-09 CN CN201710012606.0A patent/CN106754420B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106007913A (en) * | 2016-05-13 | 2016-10-12 | 安徽金农惠民生物技术有限公司 | Method for preparing compound microbial fertilizer with cyanophyta mud |
Non-Patent Citations (2)
| Title |
|---|
| 崔亚青: "蓝藻堆肥资源化利用关键技术研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 李克朗: "太湖蓝藻资源化利用可行性研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810929A (en) * | 2019-03-27 | 2019-05-28 | 江南大学 | A method of utilizing the clay standby active microbial inoculum of cyanobacteria |
| CN113862187A (en) * | 2021-10-14 | 2021-12-31 | 华南农业大学 | Method for producing agricultural microbial agent by treating pig farm biogas slurry through nitrogen-fixing blue algae and trichoderma combined and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106754420B (en) | 2021-02-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102174398B (en) | Composite microbiological bacterial agent used for returning maize straws to field and preparation method and applications thereof | |
| CN102276301B (en) | Straw decomposing agent and production method thereof | |
| CN107857689A (en) | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure | |
| CN104671860A (en) | Bioorganic fertilizer using agricultural waste as raw materials and preparation method of bioorganic fertilizer | |
| CN101255402A (en) | Thallus for producing biological fertilizer by employing cane sugar filter mud fermentation | |
| CN105884405A (en) | Preparation method of amino acid-polypeptide liquid fertilizer | |
| CN111620745B (en) | Method for producing bio-organic fertilizer by degrading agricultural wastes with biological agent | |
| CN107176891A (en) | It is a kind of to promote the biological agent and its production technology of stalk fast degradation | |
| CN108130295B (en) | Microbial organic fertilizer fermentation inoculant | |
| CN108017446A (en) | The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium | |
| CN103396182B (en) | Method for producing solanaceous vegetable seedling growing substrate by utilizing salvaged material of cassava processing | |
| CN107603916A (en) | A kind of antimicrobial composition microbial inoculum, its preparation method and application | |
| CN111533586B (en) | Chicken manure bio-organic fertilizer and preparation method thereof | |
| CN103484396A (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN108218570A (en) | A kind of liquid biological bacterial manure and preparation method thereof | |
| CN108046966A (en) | The method of composite bacteria fermentation peach branch crushed material | |
| CN110628675A (en) | Straw field-returning decomposition agent and preparation method thereof | |
| CN107840706A (en) | A kind of microbial manure and its application using the production of cassava alcohol waste water | |
| CN108863462A (en) | It is a kind of using stalk as biological organic fertilizer of raw material and preparation method thereof | |
| CN103184174A (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN112573959A (en) | Efficient and safe bio-organic fertilizer | |
| CN104293719A (en) | Fast decomposing agent for fermentation bed aging padding, organic fertilizer and production method of organic fertilizer | |
| CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
| CN105037045B (en) | A kind of soybean nutritional liquid and preparation method thereof and application method | |
| CN109134114A (en) | A kind of preparation method of organic biofertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |