CN105037045B - A kind of soybean nutritional liquid and preparation method thereof and application method - Google Patents
A kind of soybean nutritional liquid and preparation method thereof and application method Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a kind of soybean nutritional liquid and preparation method thereof and application method.The preparation method of the soybean nutritional liquid includes the following steps：(1) in parts by weight, 20 30 parts of 20 30 parts of 40 60 parts of agricultural crop straw, feces of livestock and poultry, mushroom residue respectively after crushed is taken to be mixed and stirred for uniformly, obtaining mixed material；(2) the function microbial inoculum to mixed material addition equivalent to its quality 10 20%, it is 50% ~ 65% then to add water to the mixed material moisture content, and control temperature is fermented 3 ~ 7 days at not higher than 50 DEG C；(3) in the ratio of 5 25 L/kg, into the mixed material after fermentation, spray water, collection leachates simultaneously filter, to obtain the final product.Soybean nutritional liquid of the present invention, raw material is taken from agricultural wastes, of low cost, and preparation expense is low, easy to operate, can effectively facilitate soybean growth, and improve disease resistance.
Description
Technical field
The present invention relates to agricultural technology field, and in particular to a kind of soybean nutritional liquid and preparation method thereof and application method.
Background technology
Containing multiple elements such as a certain amount of carbon, nitrogen, phosphorus, potassium, calcium, silicon in agricultural crop straw, at the same time containing abundant fibre
Tie up the organic substances such as element, hemicellulose, lignin and protein.In stalk contain for the substantial amounts of nutrition that animals and plants utilize into
Point, phosphorus content is about 40~60% in stalk, nitrogen content about 0.6 ~ 1.1%, is that the recyclable organism with comprehensive utilization value provides
Source.Wherein maize straw is a kind of important residual biomass resource in China's agricultural production, and annual output is about 2.1 hundred million
Ton, and there is ever-increasing trend.With the development of agricultural production and the transformation of life of farmers mode, and urbanization into
Journey, the surplus of maize straw increase year by year, and the maize straw for having more than half fails to be utilized effectively.Maize straw is not
Suitable disposal（Such as open incineration）, the waste of resource is not only caused, but also be easy to cause environmental problem.Part monitoring data are shown
Show, it is to cause air pollution to be particularly one of main cause of serious haze that straw from village, which burns, and China forbids outdoor burn in more ground in succession
Burn stalk.
Meanwhile Mushroom production is solid phase fermentation industry maximum in the world, and mushroom residue refers to be left after cultured mushroom
Solid waste.Due to containing substantial amounts of remaining nutrients such as carbohydrate, organic acid, bioactive substance and enzyme in mushroom residue
Deng various nutriments can be provided for the growth of microorganism.
At present, fodder, compost, anaerobic methane production, power generation/pyrolysis and making composite material etc. are stalk resource profits
Main path.Stalk aerobic compost is current rather cost-effective method, but since stalk mainly contains cellulose, half
The hard-degraded substances such as cellulose, lignin, composting cycle is longer, and substantial amounts of research at present concentrates on the micro- life for accelerating straw decomposing
Thing microbial inoculum.
Chinese patent literature《A kind of microbial bacterial agent of degrading straw》（Publication number CN102174424A, publication date
On September 7th, 2011）, a kind of microbial bacterial agent of efficient degradation crop material is disclosed, it is by bacillus licheniformis JLC38
（Bacillus licheniformis）, Wei Deng Shitaiteng bacillus JLC43（Bacillus weihenstephanensis）
Formed Deng 2 kinds of bacterial strains.
Chinese patent literature《Complex microorganism preparations and its answering in the microbial degradation of crop material》（Publication number
CN101898907A, December 01 2010 publication date）, a kind of complex microorganism preparations and its application are disclosed, wherein multiple
It is that cellulose-decomposing bacterium and surfactant formulatory form to close microorganism formulation, and cellulose-decomposing bacterium belongs to trichoderma, can increase
The degradation efficiency of stalk.
Chinese patent literature《Utilize the method for the waste material bacteria residue production microbial organic bacterial manure of stalk edible mushroom》（It is public
The number of opening CN103570383A, February 12 2014 publication date）, after disclosing a kind of production edible mushroom using stalk
The method that waste material bacteria residue produces microbial organic bacterial manure, the waste material bacteria residue after producing edible mushroom using stalk is raw material, using bacteria residue
Pretreatment, the processing step production microbial organic bacterial manure for preparing activation zymotic fluid and microorganism secondary fermentation, its product conduct
Microbial-bacterial fertilizer uses.
Although traditional straw compost technology, but provide feasible technical solution for the utilization of resources of stalk.But by
In straw compost, there are many deficiencies：（1）The compost time of stalk is still long, and the decomposed required cycle is up to the several months；（2）Compare
There is no advantage in chemical fertilizer, since straw compost fertilizer efficiency is relatively low, applied amount is larger, increase fertile cost etc..Therefore, exploitation is suitable for
Specific crops, manufacturing cycle is shorter and has specific function（Such as disease resistance）Nutrient solution, there is significant practical anticipate
Justice.
The content of the invention
It is used to promote the soybean nutritional liquid for growing, improving disease resistance it is an object of the invention to provide a kind of, to solve
The deficiency that required cycle of existing composting technology is long, fertilizer efficiency is low and amount of application is big.
To achieve the above object, the present invention provides a kind of preparation method of soybean nutritional liquid.Specifically, this method is included such as
Lower step：
(1) in parts by weight, 20-30 parts of 40-60 parts of agricultural crop straw, feces of livestock and poultry after crushed, mushroom respectively are taken
20-30 parts of slag is mixed and stirred for uniformly, obtaining mixed material；
(2) to function microbial inoculum of the mixed material addition equivalent to its quality 10-20%, the mixing is then added water to
Material water ratio is 50% ~ 65%, and control temperature, which is not higher than at 50 DEG C, ferments 3 ~ 7 days；
(3) in the ratio of 5-25 L/kg, into the mixed material after fermentation, spray water, collection leachates simultaneously filter, to obtain the final product.
Preferably, the preparation method of the soybean nutritional liquid includes the following steps：
(1) in parts by weight, 23-27 parts of 45-55 parts of agricultural crop straw, feces of livestock and poultry after crushed, mushroom respectively are taken
23-27 parts of slag is mixed and stirred for uniformly, obtaining mixed material；
(2) in parts by weight, 15 portions of function microbial inoculums are added to the mixed material, then adds water to the mixed material
Moisture content is 55% ~ 60%, and controls temperature to be not higher than at 50 DEG C and ferment 4 ~ 6 days；
(3) in the ratio of 10-20 L/kg, into the mixed material after fermentation, spray water, collection leachates simultaneously filter, i.e.,
.
The soybean nutritional liquid as made from the above method of the present invention has the function of to promote growth, improves disease resistance, especially
Biomass, root nodule numbers and the yield of soybean are improved, strengthens the biological nitrogen fixation ability of soybean, and improve anti-soybean bacterium macula
The significant effect of disease.
Wherein, the agricultural crop straw is the one or more in maize straw, wheat stalk, rice straw, is preferably
Maize straw, and particle diameter≤5cm after crushing, are preferably≤2cm；The feces of livestock and poultry is pig manure, cow dung, sheep manure, chicken manure, duck
One or more in excrement, preferably pig manure, and particle diameter≤2cm after crushing, are preferably≤1cm；After the mushroom residue crushes
Particle diameter≤2cm, be preferably≤1cm.
Wherein, mushroom residue falls the solid waste remained by mushroom for picking after cultured mushroom.
Agricultural crop straw, feces of livestock and poultry and mushroom residue used in the present invention derive from a wealth of sources, and are the money of agricultural wastes
Sourceization recycles, therefore obtained soybean nutritional liquid is cheap.
Wherein, the function microbial inoculum is made by following methods：
90-110 parts of water are added in 100 parts of solid mediums and 0.5-2 parts of ammonium sulfate sterilize, and after sterilizing, are added
8-12 parts of mixed bacteria liquids, are then placed in quiescent culture 6-8 days in 27-29 DEG C of incubator, to obtain the final product.
Preferably, the function microbial inoculum is made by following methods：
100 parts of water are added in 100 parts of solid mediums and 1 part of ammonium sulfate sterilizes, and after sterilizing, add 10 parts of mixing
Bacterium solution, is then placed in quiescent culture 7 days in 28 DEG C of incubators, to obtain the final product.
Wherein, the solid medium in parts by weight, is prepared by the raw material including following component：5-7 parts of wheat brans,
2-4 parts of chaffs, 0.5-2 parts of rice husks.It is preferred that it is made of 6 parts of wheat brans, 3 parts of chaffs and 1 part of rice husk.The solid medium raw material sources are wide
It is general, it is cheap.
Wherein, in the mixed bacteria liquid contain selected from Trichoderma viride (Trichoderma viride), aspergillus niger
(Aspergillus niger), Phomopsis (Phompsis liquidambari), bacillus subtilis (Bacillus subtilis) two kinds or more of microorganisms, wherein in mixed bacteria liquid total viable count be 0.1 ~ 10 × 1010CFU/mL.This is mixed
Close bacterium solution and be conducive to humic acid and the formation of fulvic acid material and the conversion of bioavailable phosphorus and potassium, and can be in fermentation process
In with the indigenous microorganism collective effect in mushroom residue, derive a large amount of feature fungi, bacterium and actinomyces, have optimization soil
The advantages of earth biological community structure, enhancing soybean nitrogen fixing capacity.
Preferably, it is 0.5 containing viable count ratio in the Mixed Microbes:1~4:1 Phomopsis (Phompsis liquidambari) and bacillus subtilis (Bacillus subtilis), total viable count is 0.5 ~ 6 × 10 in mixed bacteria liquid10
CFU/mL。
Invention also provides the preparation-obtained soybean nutritional liquid of the above method.The soybean nutritional liquid phase is compared with solid
Matrix is more convenient to apply and beneficial to root absorption.
In addition, the application present invention also offers above-mentioned soybean nutritional liquid in soybean is cultivated；It is preferred that the application
Application method is：Divide 1-5 times (preferably 3 times) to soybean seedling root soil with the amount of 20-100 mL/ plants (preferably 40-80mL/ plants)
Earth applies the soybean nutritional liquid.
The method of the present invention has the following advantages that：
1st, the raw material that soybean nutritional liquid of the invention uses is agricultural crop straw, feces of livestock and poultry and mushroom residue, is derived from a wealth of sources,
And recycled for the recycling of agricultural wastes, therefore, soybean nutritional liquid is cheap；
2nd, in the preparation method of soybean nutritional liquid of the present invention, agricultural crop straw, feces of livestock and poultry and mushroom residue mixture, it is only necessary to
Ferment 3 ~ 7 days in room temperature environment, the time is short, efficient, and nutrient solution is more convenient to apply compared to solid matrix；
3rd, soybean nutritional liquid of the invention, has the function of to promote soybean growth, improves soybean disease resistance.And due to this hair
Containing abundant humic acid and fulvic acid material, and the element such as phosphorus, potassium in bright nutrient solution, and it is rich in fermentation process and spreads out
Raw a large amount of feature fungi, bacterium and actinomyces, thus it is possible to vary structure of soil microbial community, improves soybean in seedling stage and flower
The soil microbe quantity of pod phase, enzymatic activity, and the biological nitrogen fixation ability of soybean, promote the root dross of soybean, improve production
Amount, and the performance of soybean resistance bacterial plaque exanthema can be improved at the same time.
Embodiment
Embodiments of the present invention are described in further detail with reference to embodiment.Following embodiments are used to illustrate this
Invention, but it is not limited to the scope of the present invention.
The present invention is raw materials used commercially available.Phomopsis is purchased from China General Microbiological culture presevation administrative center；
Trichoderma viride, black-koji mould and bacillus subtilis are purchased from Guangdong Province's Culture Collection；Wherein above-mentioned strain can
Using PDA culture medium culture, 4 DEG C of environment are stored in.
Embodiment 1
The present embodiment provides a kind of soybean nutritional liquid, wherein, the soybean nutritional liquid is prepared by the following method, specifically
Include the following steps：
(1) take crush after particle diameter≤2cm maize straw, crush after particle diameter≤1cm pig manure, crush after particle diameter≤1cm
Mushroom residue press 6:2:2 mass ratio is mixed and stirred for uniformly, obtaining mixed material；
(2) the function microbial inoculum to mixed material addition equivalent to mixed material quality 15%, then adds water to described mixed
It is 60% to close material water ratio, and control temperature is no more than fermented and cultured 6 days at 50 DEG C；
Wherein, the function microbial inoculum is prepared by the following method：In parts by weight, 100 parts of solid mediums are taken（Bran
Skin：Chaff：Rice husk is 6:3:1）, 100 parts of water are added, after then 1 part of ammonium sulfate of addition is sterilized, add 10 parts of mixed bacteria liquids,
It is then placed in quiescent culture 7 days in 28 DEG C of incubators, to obtain the final product.
Wherein, it is 3 containing viable count ratio in the mixed bacteria liquid：1 Phomopsis (Phompsis liquidambari) and bacillus subtilis (Bacillus subtilis), total viable count is 4.6 × 10 in mixed bacteria liquid10
CFU/mL。
(3) in the ratio of 15L/kg, into the mixed material after fermentation, spray water, collection leachates are simultaneously filtered with 5 micron openings
Membrane filtration, to obtain the final product.
Embodiment 2
The present embodiment provides a kind of soybean nutritional liquid, wherein, the soybean nutritional liquid is prepared by the following method, specifically
Include the following steps：
(1) take crush after particle diameter≤5cm maize straw, crush after particle diameter≤2cm pig manure, crush after particle diameter≤2cm
Mushroom residue in mass ratio 4:3:3 are mixed and stirred for uniformly, obtaining mixed material；
(2) the function microbial inoculum to mixed material addition equivalent to mixed material quality 15%, then adds water to described
Mixed material moisture content is 65%, and control temperature is not higher than fermented and cultured 5 days at 50 DEG C；
Wherein, the function microbial inoculum is prepared by the following method：In parts by weight, 100 parts of solid mediums are taken（Bran
Skin：Chaff：Rice husk is 6:3:1）, 100 parts of water are added, after then 2 parts of ammonium sulfate of addition are sterilized, add 10 parts of mixed bacteria liquids,
It is then placed in quiescent culture 8 days in 29 DEG C of incubators, to obtain the final product.
Wherein, it is 2.5 containing viable count ratio in above-mentioned mixed bacteria liquid：1：1 Phomopsis, aspergillus niger and green wood
Mould, total viable count is 6.4 × 10 in mixed bacteria liquid10 CFU/mL。
(3) in the ratio of 25L/kg, into the mixed material after fermentation, spray water, collection leachates are simultaneously filtered with 5 micron openings
Membrane filtration, to obtain the final product.
Embodiment 3
The present embodiment provides a kind of soybean nutritional liquid, wherein, the soybean nutritional liquid is prepared by the following method, specifically
Include the following steps：
(1) take crush after particle diameter≤4cm maize straw, crush after particle diameter≤1cm pig manure, crush after particle diameter≤1cm
Mushroom residue press 5:2.5:2.5 mass ratio is mixed and stirred for uniformly, obtaining mixed material；
(2) the function microbial inoculum to mixed material addition equivalent to mixed material quality 15%, then adds water to described
Mixed material moisture content is 50%, and control temperature, which is not higher than at 50 DEG C, ferments 4 days；
Wherein, the function microbial inoculum is prepared by the following method：In parts by weight, 100 parts of solid mediums are taken（Bran
Skin：Chaff：Rice husk is 6:3:1）, 100 parts of water are added, after then 1 part of ammonium sulfate of addition is sterilized, add 10 parts of mixed bacteria liquids,
It is then placed in quiescent culture 7 days in 28 DEG C of incubators, to obtain the final product.
Wherein, it is 0.5 containing viable count ratio in above-mentioned mixed bacteria liquid：1：2：2 Trichoderma viride, aspergillus niger, intend stem point
Mould and bacillus subtilis, total viable count is 7.2 × 10 in mixed bacteria liquid10 CFU/mL。
(3) in the ratio of 5L/kg, into the mixed material after fermentation, spray water, collection leachates are simultaneously filtered with 5 micron openings
Membrane filtration, to obtain the final product.
Embodiment 4
Compared with Example 1, distinctive points are only that：In the present embodiment, the function microbial inoculum is prepared by the following method：
In parts by weight, 90 parts of solid mediums are taken, add 90 parts of water, after then 0.5 part of ammonium sulfate of addition is sterilized, add 8 parts
Mixed bacteria liquid, is then placed in quiescent culture 6 days in 27 DEG C of incubators, to obtain the final product.
Embodiment 5
Compared with Example 1, distinctive points are only that:In the present embodiment, the function microbial inoculum is prepared by the following method：
In parts by weight, 110 parts of solid mediums are taken, add 110 parts of water, after then 2 parts of ammonium sulfate of addition are sterilized, add 12 parts
Mixed bacteria liquid, is then placed in quiescent culture 8 days in 29 DEG C of incubators, to obtain the final product.
Experimental example 1
The soybean varieties of this experimental example are early raw white bird, take 400 plants of soybean seedling plantations in flowerpot.Soil collection is from nothing
Polluted agricultural land, pH are about 6.0.It is divided into blank group and five groups of control groups, every group 100 plants, wherein blank group does not add soybean nutritional
Liquid, control group 1-5 apply the soybean nutritional liquid as made from embodiment 1-5 respectively, and control group is to single plant soybean seedling root soil
Divide 3 60 mL soybean nutritional liquid of application, every time 20 mL.Blank group and control group apply chemical fertilizer, and single plant applied amount is nitrogenous fertilizer
1.0 g/strain, phosphate fertilizer 2.0g/strain, KCl 2.0g/strain, K2SO41.0g/strain.
Disease-resistant sex investigation：Using the method identification blank group and control group of blade back spraying and upper blade frictional inoculation to big
The anti-sense reaction of beans bacterial speck bacterial strain, cause of disease is rape Xanthomonas campestris soybean pvs oryzae and oryzicola
（Xanthomonascampestrispv glycines）.Disease index=100 × ∑（The scab number of sheets × disease level typical value）/
(investigation total number of sheets × highest disease level value).Wherein soybean disease leaf disease index, grade scale are as follows：
0 grade：Disease-free spot;
1 grade：Lesion area accounts for whole leaf area below 5%；
2 grades：Scab leaf area accounts for the 6% ~ 10% of whole leaf area；
3 grades：Scab leaf area accounts for whole leaf area 11% ~ 25%；
4 grades：Scab leaf area accounts for whole leaf area 26% ~ 50%；
5 grades：Scab leaf area accounts for whole leaf area more than 50%.
After Soybean Seedlings Growth 60 days, the biomass of soybean, soybean yields, root nodule numbers in blank group and control group are measured
With soybean disease index, test result is calculates average value, as a result as shown in table 1- tables 5.
Table 1
| Incidence | Disease index | Plant fresh weight（Gram） | Root nodule numbers（It is a） | Total beanpod fresh weight（Gram） | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 2 | 47 | 21 | 13.5 | 129 | 91 |
Table 1 is the embodiment of the present invention 1 to promoting soybean to grow, improving the effect of soybean disease resistance.As can be seen from Table 1,
After adding soybean nutritional liquid, the plant fresh weight and root nodule numbers of soybean increase by 108% and 158% respectively, and total beanpod fresh weight is from 63 g/
Strain increases to 91g/ plants.In addition, bacterial pustule of soybean incidence is down to 47% from 86%.
Table 2
| Incidence | Disease index | Plant fresh weight（Gram） | Root nodule numbers（It is a） | Total beanpod fresh weight（Gram） | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 1 | 56 | 29 | 11 | 143 | 86 |
Table 2 is the embodiment of the present invention 2 to promoting soybean to grow, improving the effect of soybean disease resistance.As can be seen from Table 2,
After adding soybean nutritional liquid, the plant fresh weight and root nodule numbers of soybean increase by 69% and 186% respectively, and total beanpod fresh weight is from 63 g/
Strain increases to 86g/ plants.In addition, bacterial pustule of soybean incidence is down to 56% from 86%.
Table 3
| Incidence | Disease index | Plant fresh weight（Gram） | Root nodule numbers（It is a） | Total beanpod fresh weight（Gram） | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 3 | 64 | 34 | 9.7 | 87 | 79 |
Table 3 is the embodiment of the present invention 3 to promoting soybean to grow, improving the effect of soybean disease resistance.As can be seen from Table 3,
After adding soybean nutritional liquid, the plant fresh weight and root nodule numbers of soybean increase by 49% and 74% respectively, and total beanpod fresh weight is from 63 g/ plants
Increase to 79g/ plants.In addition, bacterial pustule of soybean incidence is down to 64% from 86%.
Table 4
| Incidence | Disease index | Plant fresh weight（Gram） | Root nodule numbers（It is a） | Total beanpod fresh weight（Gram） | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 2 | 51 | 24 | 13.7 | 116 | 87 |
Table 4 is the embodiment of the present invention 4 to promoting soybean to grow, improving the effect of soybean disease resistance.As can be seen from Table 4,
After adding soybean nutritional liquid, the plant fresh weight and root nodule numbers of soybean increase by 111% and 132% respectively, and total beanpod fresh weight is from 63 g/
Strain increases to 87g/ plants.In addition, bacterial pustule of soybean incidence is down to 51% from 86%.
Table 5
| Incidence | Disease index | Plant fresh weight（Gram） | Root nodule numbers（It is a） | Total beanpod fresh weight（Gram） | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 2 | 49 | 27 | 12.8 | 123 | 89 |
Table 5 is the embodiment of the present invention 5 to promoting soybean to grow, improving the effect of soybean disease resistance.As can be seen from Table 5,
After adding soybean nutritional liquid, the plant fresh weight and root nodule numbers of soybean increase by 97% and 146% respectively, and total beanpod fresh weight is from 63 g/
Strain increases to 89g/ plants.In addition, bacterial pustule of soybean incidence is down to 49% from 86%.
Although above with general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (11)
1. a kind of preparation method of soybean nutritional liquid, it is characterised in that this method comprises the following steps：
(1) in parts by weight, 20-30 parts of 40-60 parts of agricultural crop straw, feces of livestock and poultry, mushroom residue 20- respectively after crushed is taken
30 parts are mixed and stirred for uniformly, obtaining mixed material；
(2) to function microbial inoculum of the mixed material addition equivalent to its quality 10-20%, the mixed material is then added water to
Moisture content is 50% ~ 65%, and control temperature, which is not higher than at 50 DEG C, ferments 3 ~ 7 days；
(3) in the ratio of 5-25 L/kg, into the mixed material after fermentation, spray water, collection leachates simultaneously filter, to obtain the final product；
The function microbial inoculum is made by following methods：
90-110 parts of water are added in 100 parts of solid mediums and 0.5-2 parts of ammonium sulfate sterilize, and after sterilizing, add 8-12
Part mixed bacteria liquid, is then placed in quiescent culture 6-8 days in 27-29 DEG C of incubator, to obtain the final product；
In the mixed bacteria liquid containing Trichoderma viride (Trichoderma viride), aspergillus niger (Aspergillus niger)、
Phomopsis (Phompsis liquidambari), bacillus subtilis (Bacillus subtilis) two kinds or it is more
Kind, one of which for Phomopsis (Phompsis liquidambari), in the mixed bacteria liquid total viable count for 0.1 ~ 10 ×
1010 CFU/mL。
2. according to the method described in claim 1, it is characterized in that, this method comprises the following steps：
(1) in parts by weight, 23-27 parts of 45-55 parts of agricultural crop straw, feces of livestock and poultry, mushroom residue 23- respectively after crushed is taken
27 parts are mixed and stirred for uniformly, obtaining mixed material；
(2) then add water to the mixed material equivalent to the function microbial inoculum of its quality 15% to mixed material addition and contain
Water rate is 55% ~ 60%, and controls temperature to ferment 4 ~ 6 days at not higher than 50 DEG C；
(3) in the ratio of 10-20 L/kg, into the mixed material after fermentation, spray water, collection leachates simultaneously filter, to obtain the final product.
3. method according to claim 1 or 2, it is characterised in that the agricultural crop straw is maize straw, Wheat Straw
One or more in stalk, rice straw, and particle diameter≤5cm after crushing；The feces of livestock and poultry is pig manure, cow dung, sheep manure, chicken
One or more in excrement, duck excrement, and particle diameter≤2cm after crushing；Particle diameter≤2cm after the mushroom residue crushing.
4. method according to claim 1 or claim 2, it is characterised in that described be filtered into was carried out using 5 micrometer Millipore filter membranes
Filter.
5. according to the method described in claim 1, it is characterized in that：The solid medium in parts by weight, by including as follows
The raw material of component is prepared：5-7 parts of wheat brans, 2-4 parts of chaffs and 0.5-2 parts of rice husks.
6. according to the method described in claim 5, it is characterized in that：The solid medium in parts by weight, by 6 parts of wheat brans, 3
Part chaff and 1 part of rice husk are formed.
7. according to the method described in claim 1, it is characterized in that：It is 0.5 ~ 4 to contain viable count ratio in the mixed bacteria liquid:
1 Phomopsis (Phompsis liquidambari) and bacillus subtilis (Bacillus subtilis), mixed bacteria liquid
In total viable count be 0.5 ~ 6 × 1010 CFU/mL。
8. soybean nutritional liquid made from the method any one of claim 1-7.
9. application of the soybean nutritional liquid in soybean is cultivated described in claim 8.
10. application according to claim 9, it is characterised in that the application method of the application is：With 20-100 mL/
The amount of strain is divided 1-5 times applies the soybean nutritional liquid to soybean seedling root soil.
11. application according to claim 10, it is characterised in that the application method of the application is：With 40-80 mL/
The amount of strain is divided 3 times applies the soybean nutritional liquid to soybean seedling root soil.
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| CN107445777A (en) * | 2017-09-28 | 2017-12-08 | 房坤华 | A kind of duck excrement compost method suitable for soybean planting |
| CN108782132B (en) * | 2018-06-20 | 2022-03-18 | 五月阳光生物科技(浙江)有限公司 | Cultivation method of fruit rich in SOD |
| CN108440219A (en) * | 2018-06-28 | 2018-08-24 | 全椒县马塘湖农作物种植专业合作社 | A kind of soybean planting environment-friendly fertilizer and preparation method thereof |
| CN113999079A (en) * | 2021-11-22 | 2022-02-01 | 辽宁农业职业技术学院 | Biomass dissolving liquid and preparation method and application thereof |
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| CN103073343A (en) * | 2013-01-08 | 2013-05-01 | 李庆康 | Low-carbon, environment-friendly and efficient organic vegetable culture medium and preparation method thereof |
| CN104692912A (en) * | 2015-01-09 | 2015-06-10 | 广德志云笋业有限公司 | Multi-effect liquid surface fertilizer for petasites palmata |
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| 拟茎点霉B3与有机肥配施对连作草莓生长的影响;郝玉敏等;《生态学报》;20121130;第6695-6704页 * |
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