CN105037045A - Soybean nutrient solution as well as preparation and using methods of soybean nutrient solution - Google Patents
Soybean nutrient solution as well as preparation and using methods of soybean nutrient solution Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a soybean nutrient solution as well as preparation and using methods of the soybean nutrient solution. The preparation method of the soybean nutrient solution comprises the following steps: (1) respectively weighing 40-60 parts by weight of smashed crop straws, 20-30 parts by weight of poultry excrements and 20-30 parts by weight of mushroom residues, mixing and uniformly stirring to obtain a mixed material; (2) adding a functional fungicide into the mixed material, then, adding water until the mixed material contains 50-65% of water, and fermenting for 3-7 days under the condition of controlling the temperature at lower than or equal to 50 DEG C, wherein the functional fungicide accounts for 10-20% of the mass of the mixed material; and (3) spraying water into the fermented mixed material according to the ratio of 5-25L/kg, collecting leachate and filtering to obtain the soybean nutrient solution. The raw materials of the soybean nutrient solution disclosed by the invention are extracted from agricultural wastes, so that the soybean nutrient solution is low in cost and preparation expense, convenient to operate, capable of effectively promoting the growth of soybeans and improving the diseases resistance.
Description
Technical field
The present invention relates to agricultural technology field, be specifically related to a kind of soybean nutritional liquid and preparation method thereof and using method.
Background technology
Containing multiple elements such as a certain amount of carbon, nitrogen, phosphorus, potassium, calcium, silicon in agricultural crop straw, simultaneously containing organic substances such as abundant Mierocrystalline cellulose, hemicellulose, xylogen and protein.Containing a large amount of nutritive ingredient that can utilize for animals and plants in stalk, in stalk, carbon content is about 40 ~ 60%, nitrogen content about 0.6 ~ 1.1%, is the renewable biological source with comprehensive utilization value.Wherein maize straw is a kind of important residue biomass resource in China's agriculture production, and annual production is about 2.1 hundred million tons, and has ever-increasing trend.Along with the development of agriculture production and the transformation of life of farmers mode, and Development of China's Urbanization, the residual content of maize straw increases year by year, has the maize straw exceeding half to fail to be utilized effectively.The improper disposal (such as open incineration) of maize straw, not only causes the waste of resource, and easily causes environmental problem.Part monitoring data shows, and it is one of the main cause causing atmospheric pollution particularly serious haze that straw from village burns, China's many ground prohibit burning out in the open stalk in succession.
Meanwhile, Mushroom production is solid phase fermentation industry maximum in the world, and mushroom residue refers to the solid waste that cultured mushroom is left later.Owing to containing a large amount of remaining nutrition in mushroom residue as carbohydrate, organic acid, biologically active substance and enzyme etc., various nutritive substance can be provided for microbial growth.
At present, fodder, compost, anaerobic methane production, generating/pyrolysis and making matrix material etc. are the main paties that stalk resource utilizes.Stalk aerobic compost is current rather cost-effective method, but because stalk is mainly containing hard-degraded substances such as Mierocrystalline cellulose, hemicellulose, xylogen, composting cycle is longer, and large quantifier elimination concentrates on the microbiobacterial agent accelerating straw decomposing at present.
Chinese patent literature " a kind of microbiobacterial agent of degrading straw " (publication number CN102174424A, September 7 2011 publication date), disclose a kind of microbiobacterial agent of efficient degradation crop material, it is by Bacillus licheniformis JLC38(
bacilluslicheniformis), Wei Deng Shitaiteng genus bacillus JLC43(
bacillusweihenstephanensis) etc. 2 kinds of bacterial strains composition.
Chinese patent literature " complex microorganism preparations and answering in the microbiological deterioration of crop material thereof " (publication number CN101898907A, December 01 2010 publication date), disclose a kind of complex microorganism preparations and application thereof, wherein complex microorganism preparations is that cellulose-decomposing bacterium and surfactant formulatory form, cellulose-decomposing bacterium belongs to Trichoderma, can increase the degradation efficiency of stalk.
Chinese patent literature " utilizing the method for the waste material bacterium slag production microbial organic bacterial manure of stalk edible mushrooms " (publication number CN103570383A, on 02 12nd, 2014 publication date), disclose a kind of method utilizing stalk to produce the waste material bacterium slag production microbial organic bacterial manure after edible mushrooms, the waste material bacterium slag after edible mushrooms is produced for raw material with stalk, adopt the pre-treatment of bacterium slag, prepare the processing step production microbial organic bacterial manure of activation fermented liquid and microorganism Secondary Fermentation, its product uses as microbial-bacterial fertilizer.
Although traditional straw compost technology, the utilization of resources that can be stalk provides feasible technical scheme.But because straw compost exists many deficiencies: the compost time of (1) stalk is still long, the required cycle of becoming thoroughly decomposed reaches the several months; (2) there is no advantage compared to chemical fertilizer, because straw compost fertilizer efficiency is lower, applied amount is comparatively large, increase with fertile cost etc.Therefore, exploitation is applicable to specific farm crop, and preparation cycle is shorter and have the nutritive medium of specific function (as disease resistance etc.), has significant Practical significance.
Summary of the invention
The object of the present invention is to provide a kind of for growth promoting effects, the soybean nutritional liquid improving disease resistance, in order to solve the deficiency that needed for existing composting technology, the cycle is long, fertilizer efficiency is low and amount of application is large.
For achieving the above object, the invention provides a kind of preparation method of soybean nutritional liquid.Particularly, the method comprises the steps:
(1) with parts by weight, get agricultural crop straw 40-60 part after crushed, feces of livestock and poultry 20-30 part, mushroom residue 20-30 part respectively and mix and stir, obtain mixture;
(2) add the function microbial inoculum being equivalent to its quality 10-20% to described mixture, then adding water to described mixture water ratio is 50% ~ 65%, and control temperature is not higher than 50 DEG C of bottom fermentations 3 ~ 7 days;
(3) in the ratio of 5-25L/kg to spray water in the mixture after fermentation, collect and drench fluid and also filter, to obtain final product.
Preferably, the preparation method of described soybean nutritional liquid comprises the steps:
(1) with parts by weight, get agricultural crop straw 45-55 part after crushed, feces of livestock and poultry 23-27 part, mushroom residue 23-27 part respectively and mix and stir, obtain mixture;
(2) with parts by weight, add 15 portions of function microbial inoculums to described mixture, then adding water to described mixture water ratio is 55% ~ 60%, and control temperature is not higher than 50 DEG C of bottom fermentations 4 ~ 6 days;
(3) in the ratio of 10-20L/kg to spray water in the mixture after fermentation, collect and drench fluid and also filter, to obtain final product.
The soybean nutritional liquid obtained by aforesaid method of the present invention has growth promoting effects, improves the function of disease resistance, especially the biomass of soybean, root nodule numbers and output is improved, strengthen the biological nitrogen fixation ability of soybean, and improve the Be very effective of Chinese People's Anti-Japanese Military and Political College's beans bacterial plaque exanthema.
Wherein, described agricultural crop straw is one or more in maize straw, wheat stalk, rice straw, is preferably maize straw, and the particle diameter≤5cm after pulverizing, be preferably≤2cm; Described feces of livestock and poultry is one or more in pig manure, cow dung, sheep excrement, chicken manure, duck excrement, is preferably pig manure, and the particle diameter≤2cm after pulverizing, be preferably≤1cm; Particle diameter≤2cm after described mushroom residue pulverizing, is preferably≤1cm.
Wherein, mushroom residue is for plucking the solid waste remained by mushroom after cultured mushroom.
Agricultural crop straw used in the present invention, feces of livestock and poultry and mushroom residue wide material sources, and the resource utilization recycling being agricultural wastes, therefore obtained soybean nutritional liquid is cheap.
Wherein, described function microbial inoculum is obtained by following methods:
In 100 parts of solid mediums, add 90-110 part water and 0.5-2 part ammonium sulfate carries out sterilizing, after sterilizing, add 8-12 part mixed bacteria liquid, then put into 27-29 DEG C of incubator quiescent culture 6-8 days, to obtain final product.
Preferably, described function microbial inoculum is obtained by following methods:
In 100 parts of solid mediums, add 100 parts of water and 1 part of ammonium sulfate carries out sterilizing, after sterilizing, add 10 parts of mixed bacteria liquids, then put into 28 DEG C of incubator quiescent culture 7 days, to obtain final product.
Wherein, described solid medium, with parts by weight, is prepared from by the raw material comprising following component: 5-7 part wheat bran, 2-4 part chaff, 0.5-2 part rice husk.Preferably be made up of 6 parts of wheat brans, 3 parts of chaffs and 1 part of rice husk.These solid medium raw material sources are extensive, cheap.
Wherein, in described mixed bacteria liquid containing be selected from viride (
trichodermaviride), aspergillus niger (
aspergillusniger), Phomopsis (
phompsisliquidambari), subtilis (
bacillussubtilis) two kinds or more of microorganisms, wherein in mixed bacteria liquid, total viable count is 0.1 ~ 10 × 10
10cFU/mL.This mixed bacteria liquid be conducive to humic acid and fulvic acid material formation and can the conversion of biological utilisation phosphorus and potassium, and can during the fermentation with the indigenous microorganism acting in conjunction in mushroom residue, derive a large amount of functional fungi, bacterium and actinomycetes, there is the advantage optimized structure of soil microbial community, strengthen soybean nitrogen fixing capacity.
Preferably, in described mixed bacterium containing viable count ratio be 0.5:1 ~ 4:1 Phomopsis (
phompsisliquidambari) and subtilis (
bacillussubtilis), in mixed bacteria liquid, total viable count is 0.5 ~ 6 × 10
10cFU/mL.
Invention also provides the preparation-obtained soybean nutritional liquid of aforesaid method.This soybean nutritional liquid is used compared to solid substrate is more convenient and is beneficial to root absorption.
In addition, present invention also offers the application of above-mentioned soybean nutritional liquid in soybean is cultivated; The using method of preferred described application is: divide 1-5 time (preferably 3 times) to apply described soybean nutritional liquid to soybean seedling root soil with the amount of 20-100mL/ strain (preferred 40-80mL/ strain).
The inventive method tool has the following advantages:
1, the raw material that soybean nutritional liquid of the present invention uses is agricultural crop straw, feces of livestock and poultry and mushroom residue, wide material sources, and be that the resource utilization of agricultural wastes recycles, and therefore, soybean nutritional liquid is cheap;
In the preparation method of 2, soybean nutritional liquid of the present invention, agricultural crop straw, feces of livestock and poultry and mushroom residue mixture, only need ferment 3 ~ 7 days at room temperature environment, the time is short, efficiency is high, and nutritive medium is used compared to solid substrate is more convenient;
3, soybean nutritional liquid of the present invention, has the function promoting soybeans they grow, improve Soybean Resistance characteristic of disease.And owing to containing abundant humic acid and fulvic acid material in nutritive medium of the present invention, and the element such as phosphorus, potassium, and be rich in a large amount of functional fungi derivative in fermenting process, bacterium and actinomycetes, structure of soil microbial community can be changed, improve soybean in seedling stage and soil microbe quantity, the enzymic activity of spending the pod phase, and the biological nitrogen fixation ability of soybean, promote the root dross of soybean, improve output, and the performance of soybean opposing bacterial plaque exanthema can be improved simultaneously.
Embodiment
Below in conjunction with embodiment, embodiments of the present invention are described in further detail.Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The present invention is raw materials used all can buy.Phomopsis can purchased from China General Microbiological culture presevation administrative center; Viride, black-koji mould and subtilis can purchased from Guangdong Province's Culture Collection; Wherein above-mentioned bacterial classification can use PDA culture medium culturing, is stored in 4 DEG C of environment.
embodiment 1
The present embodiment provides a kind of soybean nutritional liquid, and wherein, described soybean nutritional liquid prepares by the following method, specifically comprises the steps:
(1) after getting the maize straw pulverizing rear particle diameter≤2cm, the pig manure pulverizing rear particle diameter≤1cm, pulverizing, the mushroom residue of particle diameter≤1cm mixes by the mass ratio of 6:2:2 and stirs, and obtains mixture;
(2) add the function microbial inoculum being equivalent to mixture quality 15% to described mixture, then adding water to described mixture water ratio is 60%, and control temperature is no more than 50 DEG C of bottom fermentations and cultivates 6 days;
Wherein, described function microbial inoculum prepares by the following method: with parts by weight, get 100 parts of solid mediums (wheat bran: chaff: rice husk is 6:3:1), add 100 parts of water, then add after 1 part of ammonium sulfate carries out sterilizing, add 10 parts of mixed bacteria liquids, then put into 28 DEG C of incubator quiescent culture 7 days, to obtain final product.
Wherein, in described mixed bacteria liquid containing viable count ratio be 3:1 Phomopsis (
phompsisliquidambari) and subtilis (
bacillussubtilis), in mixed bacteria liquid, total viable count is 4.6 × 10
10cFU/mL.
(3) in the ratio of 15L/kg to spray water in the mixture after fermentation, collect and drench fluid with 5 micron openings membrane filtrations, to obtain final product.
embodiment 2
The present embodiment provides a kind of soybean nutritional liquid, and wherein, described soybean nutritional liquid prepares by the following method, specifically comprises the steps:
(1) get pulverize after particle diameter≤5cm maize straw, pulverize after particle diameter≤2cm pig manure, pulverize after particle diameter≤2cm mushroom residue in mass ratio 4:3:3 mix and stir, obtain mixture;
(2) add the function microbial inoculum being equivalent to mixture quality 15% to described mixture, then adding water to described mixture water ratio is 65%, and control temperature does not cultivate 5 days higher than 50 DEG C of bottom fermentations;
Wherein, described function microbial inoculum prepares by the following method: with parts by weight, get 100 parts of solid mediums (wheat bran: chaff: rice husk is 6:3:1), add 100 parts of water, then add after 2 parts of ammonium sulfate carry out sterilizing, add 10 parts of mixed bacteria liquids, then put into 29 DEG C of incubator quiescent culture 8 days, to obtain final product.
Wherein, contain Phomopsis, aspergillus niger and viride that viable count ratio is 2.5:1:1 in above-mentioned mixed bacteria liquid, in mixed bacteria liquid, total viable count is 6.4 × 10
10cFU/mL.
(3) in the ratio of 25L/kg to spray water in the mixture after fermentation, collect and drench fluid with 5 micron openings membrane filtrations, to obtain final product.
embodiment 3
The present embodiment provides a kind of soybean nutritional liquid, and wherein, described soybean nutritional liquid prepares by the following method, specifically comprises the steps:
(1) after getting the maize straw pulverizing rear particle diameter≤4cm, the pig manure pulverizing rear particle diameter≤1cm, pulverizing, the mushroom residue of particle diameter≤1cm mixes by the mass ratio of 5:2.5:2.5 and stirs, and obtains mixture;
(2) add the function microbial inoculum being equivalent to mixture quality 15% to described mixture, then adding water to described mixture water ratio is 50%, and control temperature is not higher than 50 DEG C of bottom fermentations 4 days;
Wherein, described function microbial inoculum prepares by the following method: with parts by weight, get 100 parts of solid mediums (wheat bran: chaff: rice husk is 6:3:1), add 100 parts of water, then add after 1 part of ammonium sulfate carries out sterilizing, add 10 parts of mixed bacteria liquids, then put into 28 DEG C of incubator quiescent culture 7 days, to obtain final product.
Wherein, be the viride of 0.5:1:2:2, aspergillus niger, Phomopsis and subtilis containing viable count ratio in above-mentioned mixed bacteria liquid, in mixed bacteria liquid, total viable count is 7.2 × 10
10cFU/mL.
(3) in the ratio of 5L/kg to spray water in the mixture after fermentation, collect and drench fluid with 5 micron openings membrane filtrations, to obtain final product.
embodiment 4
Compared with embodiment 1, distinctive points is only: in the present embodiment, described function microbial inoculum prepares by the following method: with parts by weight, get 90 parts of solid mediums, add 90 parts of water, then add after 0.5 part of ammonium sulfate carries out sterilizing, add 8 parts of mixed bacteria liquids, then put into 27 DEG C of incubator quiescent culture 6 days, to obtain final product.
embodiment 5
Compared with embodiment 1, distinctive points is only: in the present embodiment, described function microbial inoculum prepares by the following method: with parts by weight, get 110 parts of solid mediums, add 110 parts of water, then add after 2 parts of ammonium sulfate carry out sterilizing, add 12 parts of mixed bacteria liquids, then put into 29 DEG C of incubator quiescent culture 8 days, to obtain final product.
experimental example 1
The soybean varieties of this experimental example is raw white bird morning, gets 400 strain soybean seedlings and plants in flowerpot.Soil collecting is from pollution-free farmland, and pH is about 6.0.Be divided into blank group and five groups of control groups, often organize 100 strains, its empty group does not add soybean nutritional liquid, and control group 1-5 uses the soybean nutritional liquid obtained by embodiment 1-5 respectively, control group divides 3 applying 60mL soybean nutritional liquid to individual plant soybean seedling root soil, each 20mL.Blank group and control group all apply chemical fertilizer, and individual plant applied amount is nitrogenous fertilizer 1.0g/ strain, phosphate fertilizer 2.0g/ strain, KCl2.0g/ strain, K
2sO
41.0g/ strain.
Disease resistance is investigated: adopt the blank group of method qualification of blade back spraying and upper blade frictional inoculation and the anti-sense of control group to bacterial pustule of soybean bacterial strain to react, cause of disease be rape Xanthomonas campestris soybean pvs oryzae and oryzicola (
xanthomonascampestrispvglycines).Disease index=100 × ∑ (the scab number of sheets × sick level typical value)/(investigating total number of sheets × the highest sick level value).The wherein sick leaf disease index of soybean, grade scale is as follows:
0 grade: without scab;
1 grade: lesion area accounts for whole leaf area less than 5%;
2 grades: scab leaf area accounts for 6% ~ 10% of whole leaf area;
3 grades: scab leaf area accounts for whole leaf area 11% ~ 25%;
4 grades: scab leaf area accounts for whole leaf area 26% ~ 50%;
5 grades: scab leaf area accounts for whole leaf area more than 50%.
Soybean Seedlings Growth is after 60 days, and measure the biomass of soybean in blank group and control group, soybean yields, root nodule numbers and soybean disease index, test result is calculating mean value, and result is as shown in table 1-table 5.
Table 1
| Sickness rate | Disease index | Plant fresh weight (gram) | Root nodule numbers (individual) | Total beanpod fresh weight (gram) | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 2 | 47 | 21 | 13.5 | 129 | 91 |
Table 1 is that the embodiment of the present invention 1 is to promoting soybeans they grow, improving the effect of Soybean Resistance characteristic of disease.As can be seen from Table 1, after adding soybean nutritional liquid, the plant fresh weight of soybean and root nodule numbers increase by 108% and 158% respectively, and total beanpod fresh weight is increased to 91g/ strain from 63g/ strain.In addition, bacterial pustule of soybean sickness rate is down to 47% from 86%.
Table 2
| Sickness rate | Disease index | Plant fresh weight (gram) | Root nodule numbers (individual) | Total beanpod fresh weight (gram) | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 1 | 56 | 29 | 11 | 143 | 86 |
Table 2 is that the embodiment of the present invention 2 is to promoting soybeans they grow, improving the effect of Soybean Resistance characteristic of disease.As can be seen from Table 2, after adding soybean nutritional liquid, the plant fresh weight of soybean and root nodule numbers increase by 69% and 186% respectively, and total beanpod fresh weight increases to 86g/ strain from 63g/ strain.In addition, bacterial pustule of soybean sickness rate is down to 56% from 86%.
Table 3
| Sickness rate | Disease index | Plant fresh weight (gram) | Root nodule numbers (individual) | Total beanpod fresh weight (gram) | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 3 | 64 | 34 | 9.7 | 87 | 79 |
Table 3 is that the embodiment of the present invention 3 is to promoting soybeans they grow, improving the effect of Soybean Resistance characteristic of disease.As can be seen from Table 3, after adding soybean nutritional liquid, the plant fresh weight of soybean and root nodule numbers increase by 49% and 74% respectively, and total beanpod fresh weight increases to 79g/ strain from 63g/ strain.In addition, bacterial pustule of soybean sickness rate is down to 64% from 86%.
Table 4
| Sickness rate | Disease index | Plant fresh weight (gram) | Root nodule numbers (individual) | Total beanpod fresh weight (gram) | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 2 | 51 | 24 | 13.7 | 116 | 87 |
Table 4 is that the embodiment of the present invention 4 is to promoting soybeans they grow, improving the effect of Soybean Resistance characteristic of disease.As can be seen from Table 4, after adding soybean nutritional liquid, the plant fresh weight of soybean and root nodule numbers increase by 111% and 132% respectively, and total beanpod fresh weight is increased to 87g/ strain from 63g/ strain.In addition, bacterial pustule of soybean sickness rate is down to 51% from 86%.
Table 5
| Sickness rate | Disease index | Plant fresh weight (gram) | Root nodule numbers (individual) | Total beanpod fresh weight (gram) | |
| Blank group | 86 | 57 | 6.5 | 50 | 63 |
| Control group 2 | 49 | 27 | 12.8 | 123 | 89 |
Table 5 is that the embodiment of the present invention 5 is to promoting soybeans they grow, improving the effect of Soybean Resistance characteristic of disease.As can be seen from Table 5, after adding soybean nutritional liquid, the plant fresh weight of soybean and root nodule numbers increase by 97% and 146% respectively, and total beanpod fresh weight is increased to 89g/ strain from 63g/ strain.In addition, bacterial pustule of soybean sickness rate is down to 49% from 86%.
Although the present invention is described in detail above to have used general explanation and specific embodiment, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a preparation method for soybean nutritional liquid, is characterized in that, the method comprises the following steps:
(1) with parts by weight, get agricultural crop straw 40-60 part after crushed, feces of livestock and poultry 20-30 part, mushroom residue 20-30 part respectively and mix and stir, obtain mixture;
(2) add the function microbial inoculum being equivalent to its quality 10-20% to described mixture, then adding water to described mixture water ratio is 50% ~ 65%, and control temperature is not higher than 50 DEG C of bottom fermentations 3 ~ 7 days;
(3) in the ratio of 5-25L/kg to spray water in the mixture after fermentation, collect and drench fluid and also filter, to obtain final product.
2. method according to claim 1, is characterized in that, the method comprises the steps:
(1) with parts by weight, get agricultural crop straw 45-55 part after crushed, feces of livestock and poultry 23-27 part, mushroom residue 23-27 part respectively and mix and stir, obtain mixture;
(2) add the function microbial inoculum being equivalent to its quality 15% to described mixture, then adding water to described mixture water ratio is 55% ~ 60%, and control temperature is not higher than 50 DEG C of bottom fermentations 4 ~ 6 days;
(3) in the ratio of 10-20L/kg to spray water in the mixture after fermentation, collect and drench fluid and also filter, to obtain final product.
3. method according to claim 1 and 2, is characterized in that, described agricultural crop straw is one or more in maize straw, wheat stalk, rice straw, and the particle diameter≤5cm after pulverizing; Described feces of livestock and poultry is one or more in pig manure, cow dung, sheep excrement, chicken manure, duck excrement, and the particle diameter≤2cm after pulverizing; Particle diameter≤2cm after described mushroom residue pulverizing.
4. method according to claim 1 or 2, is characterized in that, described in be filtered into employing 5 micrometer Millipore filtering membrane and filter.
5. method according to claim 1 or 2, is characterized in that, described function microbial inoculum is obtained by following methods:
In 100 parts of solid mediums, add 90-110 part water and 0.5-2 part ammonium sulfate carries out sterilizing, after sterilizing, add 8-12 part mixed bacteria liquid, then put into 27-29 DEG C of incubator quiescent culture 6-8 days, to obtain final product.
6. method according to claim 5, is characterized in that: described solid medium, with parts by weight, is prepared from by the raw material comprising following component: 5-7 part wheat bran, 2-4 part chaff and 0.5-2 part rice husk; Preferably be made up of 6 parts of wheat brans, 3 parts of chaffs and 1 part of rice husk.
7. method according to claim 5, is characterized in that: in described mixed bacteria liquid containing viride (
trichodermaviride), aspergillus niger (
aspergillusniger), Phomopsis (
phompsisliquidambari), subtilis (
bacillussubtilis) two kinds or more of, in described mixed bacteria liquid, total viable count is 0.1 ~ 10 × 10
10cFU/mL.
8. method according to claim 7, is characterized in that: in described mixed bacteria liquid containing viable count ratio be 0.5:1 ~ 4:1 Phomopsis (
phompsisliquidambari) and subtilis (
bacillussubtilis), in mixed bacteria liquid, total viable count is 0.5 ~ 6 × 10
10cFU/mL.
9. the soybean nutritional liquid that the method according to any one of claim 1-8 is obtained.
10. the application of soybean nutritional liquid according to claim 9 in soybean is cultivated: the using method of preferred described application is: divide 1-5 time (preferably 3 times) to apply described soybean nutritional liquid to soybean seedling root soil with the amount of 20-100mL/ strain (preferred 40-80mL/ strain).
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| CN115196999A (en) * | 2018-06-20 | 2022-10-18 | 五月阳光生物科技(浙江)有限公司 | Bacteria, fertilizer and method for cultivating peach rich in SOD (superoxide dismutase) |
| CN108440219A (en) * | 2018-06-28 | 2018-08-24 | 全椒县马塘湖农作物种植专业合作社 | A kind of soybean planting environment-friendly fertilizer and preparation method thereof |
| CN113999079A (en) * | 2021-11-22 | 2022-02-01 | 辽宁农业职业技术学院 | Biomass dissolving liquid and preparation method and application thereof |
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