CN104073445B - A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation - Google Patents
A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation Download PDFInfo
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- CN104073445B CN104073445B CN201410257058.4A CN201410257058A CN104073445B CN 104073445 B CN104073445 B CN 104073445B CN 201410257058 A CN201410257058 A CN 201410257058A CN 104073445 B CN104073445 B CN 104073445B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/20—Sludge processing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Soil Conditioners And Soil-Stabilizing Materials (AREA)
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Abstract
The invention discloses a kind of stalk and pre-process microbial bacterial agent with ight soil biogas fermentation, following bacterial classification collectively constitute: Penicillum glaucum 2 ~ 8 × 1010Cfu/g, Rhizopus oryzae 2 ~ 8 × 1010Cfu/g, Phanerochaete chrysosporium 2 ~ 8 × 1010Cfu/g, aspergillus flavus 2 ~ 8 × 1010Cfu/g, spiral shell spore bacterium 2 ~ 8 × 1010cfu/g.This microbial bacterial agent, can promote stalk ight soil hybrid anaerobic fermentation process, shortens fermentation period, it is possible to acceleration raw material is organic biodegradable, and the preparation method of microbial inoculum is simple, it is easy to implement, and in unit raw material, micro-flora content is big, and activity is high.Can be applicable to biogas fermentation enterprise for different ight soil and the fermentation of stalk so that ight soil is innoxious with stalk, minimizing, resource.
Description
Technical field
The invention belongs to agricultural waste fermentation pretreatment microbial inoculum technical field, be specifically related to a kind of stalk and pre-process microbial bacterial agent with ight soil biogas fermentation.
Background technology
At present, the stalk of the annual output of China about 700,000,000 tons, livestock and poultry breeding industry ight soil discharge capacity about 17.3 hundred million tons.Substantial amounts of agricultural wastes can not get reasonably utilizing, and is mostly directly burned or be discarded in field, river, the most not only causes the waste of resource but also ecological environment causes serious destruction.Research shows, is about 0.45m with stalk for fermenting raw materials production potential3/ (Kg Ts), suitable with manure fermentation rate, its production potential is the hugest.Produce biogas with stalk for fermenting raw materials, both solved the problem that rural energy is not enough, opened a new approach for solution " crop straw burning " problem again.Biogas residue after biogas fermentation can produce high-quality biogas residue organic fertilizer simultaneously, is used for improving soil physical and chemical property, increases soil fertility, and can reduce the use of chemical fertilizer, promotes ecological agriculture construction.But owing to stalk being mainly composed of cellulose, hemicellulose and lignin, if by direct for stalk pit entry fermentation, the most destroyed and easy floating crust of wax coat on stalk surface, causing aerogenesis the best.Therefore needed it is pre-processed before stalk enters pond.In recent years, correlative study about degrading straw is reported more, but it is mostly focused on the research of physics, chemical method and enzyme solution etc., deeply carry out the screening of straw degradative bacterium, identify and the research combined is less, and the straw degradative microbial inoculum that at present market is conventional mostly is the composite bacteria agent in applicable field, rather than the special bacteria agent of stalk marsh gas Biological Pretreatment.Stalk and ight soil biogas fermentation pretreatment microbial bacterial agent is also faced with problems with at present:
(1) source of microorganism is the most extensive.
Microbe species is various, distributed more widely, the most nearly ubiquitous.The most place of germs collect is soil, and soil is the base camp of various microbial growth, arbitrarily takes handful of soil or a soil, it is simply that a microbial world 1, no matter quantity or kind are the most a lot.In fertile soil, every gram of soil contains 2,000,000,000 microorganisms, even dead soil, also contains hundred million microorganisms of 3-5 in every gram of soil.Up to now, our known microorganism there are about 100,000 kinds, but only developed in human being's production and life and had been found that the 1% of microbial species number.When laboratory gathers bacterial classification sample, it then follows principle be that the source of material is the most extensive, more likely obtain new bacterial classification.Therefore, development of new efficient biogas fermentation pretreatment microbial inoculum, it is necessary to extensively draw materials, and set certain specific environmental factor in laboratory, such as temperature, the characteristic etc. of selected materials, the most just can filter out microorganism fungus kind that is substantial amounts of and that contribute to sweat.
(2) composition of microbial bacterial agent is simple
Microbial bacterial agent is to screen simple function bacterium at present, or is simply compounded by difference in functionality bacterium.There is bigger problem in this mode: first these single bacterium really have certain function when laboratory cultures, but at the complex condition of fermentation, are but extremely difficult to ideal effect.This is owing to many single fungus strains can not growth and breeding or be easily limited by the growth of other microorganisms in the actual environment；Planting interact secondly as there is many between different micropopulations, the single bacterium of certain function that what laboratory screening got have can not fully prove that it has identical effect in a complicated colony；3rd, some microorganism biogas fermentation microbial inoculum, simply fermentation strain is simply mixed, to the antagonism research between bacterial strain not thoroughly, which results in microorganism fungus kind and do not reach desired effect due to the antagonism between bacterial strain during the fermentation；4th, it is impossible to effectively utilize the effect of the various enzymes that microorganism produces.In organic degradation process, the degradation of enzyme is the most crucial, therefore, comprises only microorganism, and the microbial bacterial agent not containing enzyme then can not play bigger effect.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, it is an object of the invention to provide a kind of stalk and pre-process microbial bacterial agent with ight soil biogas fermentation, promote stalk ight soil mixing anaerobic biogas fermentation process, shorten fermentation period, accelerate raw material organic biodegradable.For different ight soil and the fermentation of stalk, make that ight soil and stalk be innoxious, minimizing, resource.It is a further object of the present invention to provide the application of mentioned microorganism microbial inoculum.
Technical scheme: in order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of stalk pre-processes microbial bacterial agent with ight soil biogas fermentation, following bacterial classification collectively constitute: Penicillum glaucum (Penicillium
glaucum) 2 ~ 8 × 1010Cfu/g, Rhizopus oryzae (Rhizopus oryzae) 2 ~ 8 × 1010Cfu/g, Phanerochaete chrysosporium (Phanerochaete
chrysosporium) 2 ~ 8 × 1010Cfu/g, aspergillus flavus (Aspergillus
flavus) 2 ~ 8 × 1010Cfu/g, spiral shell spore bacterium (Spirillospora) 2 ~ 8 × 1010cfu/g。
A kind of stalk and ight soil biogas fermentation pre-process the fermentation process of microbial bacterial agent, specific as follows:
1) glucose is dissolved in water, adds microbial inoculum and fully stir evenly, microbial inoculum is melted, then dilution is uniformly splashed on stalk, and fully stir uniformly；
2) stalk after step 1) being processed puts in fermentation tank, adds ight soil, carries out the producing methane through anaerobic fermentation of stalk and ight soil；
Wherein, the part by weight composition of material: ight soil 20% ~ 25%, stalk 1% ~ 2%, glucose 0.01% ~ 0.02%, microbial bacterial agent 0.01% ~ 0.02%, running water 70% ~ 80%；Material total moisture should control 90 ~ 92%, and pH is in neutral or alkalescent, and material particular diameter is at 5 ~ 20mm.
By regulating the pH value of material with soda lime or acetic acid.
Fermentation temperature is 37 DEG C, and every day stirs 1-2 time.
Microbial bacterial agent is 1:50 with the mass ratio of stalk.
Beneficial effect: compared with prior art, the stalk of the present invention pre-processes microbial bacterial agent with ight soil biogas fermentation, stalk ight soil hybrid anaerobic fermentation process can be promoted, shorten fermentation period, can speed up raw material organic biodegradable, and the preparation method of microbial inoculum is simple, it is easy to implement, in unit raw material, micro-flora content is big, and activity is high.Can be applicable to biogas fermentation enterprise for different ight soil and the fermentation of stalk so that ight soil is innoxious with stalk, minimizing, resource.There are following performance indications:
(1) product appearance and the shelf-life: product is khaki powder, odorless, shady and cool (less than the 20 DEG C) place of being dried preserves, the term of validity 1 year.
(2) use the fermentation maturity phase of microbial inoculum: stalk (mixing with ight soil) is 10-16 days.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
Microorganism in stalk and ight soil biogas fermentation pretreatment the microbial bacterial agent mainly various cellulose-degrading bacterias from fermentation biogas slurry, lignin-degrading bacteria, the screening such as various bacteriums in ripe compost, trees soil, actinomyces, fungi and obtain.
(1) separation of microorganism and screening
Cellulose-degrading bacteria, the separation of lignin-degrading bacteria and screening, it is microbe-derived for taking the existing marsh-gas fermentation tank in laboratory, sole carbon source is made with stalk powder, preparation minimal medium, dilution-plate method is used to separate different microorganisms, 32 DEG C of cultivations, isolate aerobic and each 12 strains of inclusive type anaerobic bacteria.Then, with every kind of microorganism, stalk is pre-processed, by measure its to cellulose, hemicellulose, the overall degradation rate of lignin, filter out that effect is higher six kinds, numbered h4, h10, h13, y2, y7 and y11.
Various bacteriums, actinomyces, the separation of fungi and screening in ripe compost, trees soil, taking compost with soil is bacterial classification source, using dilution-plate method to separate different microorganisms, wherein culture medium is respectively adopted beef-protein medium, Gause I culture medium, Cha Shi culture medium and PDA culture medium and respectively is isolated by bacterium, actinomyces, mould and fungi.32 DEG C of cultivations, and measured by increment, filter out the microorganism that increment is maximum respectively, and number consecutively is n4, n6, g1, g8, zd5, zd10, zt4, m2, m9 and m10.
(2) microorganism is sieved again
The above-mentioned 16 kinds of microbial inoculants filtered out to the inorganic salts liquid culture medium make with stalk powder sole carbon source will grow, then measure cellulase activity and the laccase activity of bacterium solution.Live size by comparing enzyme, filter out that Enzyme activity is bigger 12 kinds: h4, h10, h13, y2, y11, m9, m10, zd5, zd10, zt4, g1 and g8.
(3) antagonistic experiment between different microorganisms
The above-mentioned 12 kinds of microorganisms filtered out are inoculated in agar nutrition flat board two-by-two, 30 DEG C of illumination cultivation, through the cultivation of three days, observe the growing state of different microorganisms, if it find that wherein there is microorganism to grow, then illustrate that its growth is suppressed by other microorganisms, thus can filter out competitive strong microorganism.Experimental result finds, competitive strong microorganism is 10 kinds therein: h4, h10, h13, y2, y11, m9, m10, zd10, g1 and g8.
(4) bacterial classification Experiment of Compatibility
The above-mentioned 10 kinds of microorganisms filtered out are carried out compatibility, has 12 groups (such as tables 1).
Table 1 bacterial strain compatibility
These 12 groups of microorganisms are inoculated into respectively the mixed-culture medium (mass ratio of basal medium and wheat bran, culture medium: wheat bran=10:1) in, carrying out common fermentation and expand cultivation, condition of culture is shaking table shaken cultivation, 30 DEG C of illumination, then measure cellulase activity and the laccase activity of bacterium solution.By comparing enzyme size alive, filter out 4 groups of microorganism compatibilities that Enzyme activity is bigger.It is centrifuged nutrient solution again separating (5000r/min rotating speed), obtain microbial cells, last vacuum drying 5h at 60 DEG C, obtaining 4 kinds of microbial bacterial agents, it is respectively No. 2 microbial inoculums (containing bacterium m10, g8, h13, g1 and y2), No. 6 microbial inoculums (containing bacterium y11, h13, zd10, h10 and g1), No. 8 microbial inoculums (containing bacterium m10, h8, zd10 and h10) and No. 12 microbial inoculums (containing bacterium m10, m9, h4, h13 and h10).
Product is khaki powder, odorless, and shady and cool (less than the 20 DEG C) place of being dried preserves, the term of validity 1 year.
Embodiment 2
Stalk is pre-processed by 4 kinds of microbial bacterias of Application Example 1 preparation, then by carrying out anaerobic fermentation in pretreated stalk input fermentation tank, filters out the one that gas production is the highest.Specific as follows:
1) weight of material ratio composition
(wet) ight soil 20% ~ 25%, stalk 1% ~ 2%, glucose 0.01% ~ 0.02%, microbial bacterial agent 0.01% ~ 0.02%, running water 70% ~ 80%.
2) manufacturing process
(1) activation process of microbial inoculum
First glucose is dissolved in water, adds microbial inoculum and fully stir evenly, microbial inoculum is melted, then dilution is uniformly splashed on stalk, and fully stir (with mixer) uniformly.
(2) sweat
Pretreated stalk is put in fermentation tank and carries out anaerobic fermentation.With the identical material that is not added with microbial bacterial agent for comparison, carry out stalk and ferment with swine waste marsh gas, the size of contrast gas production every day.
Material moisture should control 90 ~ 92%, and pH is in neutral or alkalescent, and material particular diameter is at 5 ~ 20mm.During anaerobic fermentation, microbial inoculum, the stalk smashed, pig manure to be thoroughly mixed with running water.The end of collecting methane pipe is submerged in water.Fermentation tank is placed in 37 DEG C of thermostats, and from fermentation, every day stirs 1-2 time certainly.
By regulating the pH value of material with soda lime or acetic acid.
Gas production size is shown in Fig. 1, the stalk of the visible present invention can promote stalk ight soil hybrid anaerobic fermentation process with ight soil biogas fermentation pretreatment microbial bacterial agent (No. 6 microbial inoculums), shorten fermentation period (10-16 days), can speed up raw material organic biodegradable, and the preparation method of microbial inoculum is simple, easy to implement, in unit raw material, micro-flora content is big, and activity is high.Can be applicable to biogas fermentation enterprise for different ight soil and the fermentation of stalk so that ight soil is innoxious with stalk, minimizing, resource.
Finally, 5 kinds of microorganisms in No. 6 microorganism compatibilities are identified, its be respectively as follows: Penicillum glaucum (Penicillium glaucum) 2 ~ 8 × 1010Cfu/g, Rhizopus oryzae (Rhizopus oryzae) 2 ~ 8 × 1010Cfu/g, Phanerochaete chrysosporium (Phanerochaete
chrysosporium) 2 ~ 8 × 1010Cfu/g, aspergillus flavus (Aspergillus
flavus) 2 ~ 8 × 1010Cfu/g, spiral shell spore bacterium (Spirillospora) 2 ~ 8 × 1010cfu/g.It is common bacterial classification.
Embodiment 3
The advantage microbial inoculum (No. 6 microorganisms of embodiment 2) filtered out according to gas production size, is mixed with stalk by different proportion, pre-processes, put in 37 DEG C of constant incubators, anaerobic fermentation.By the size of methane quantity, finally determine the optimum charging ratio (mass ratio) of microbial inoculum and stalk.
The mass ratio of dry bacteria and stalk takes No. 1 1:1000, No. 2 1:500, No. 3 1:100, No. 4 1:50 and No. 5 1:20 respectively, and wherein stalk is unified adds 15g.
Experiment shows: when the mass ratio of dry bacteria and stalk is 1:50, gas production is the highest.Therefore, selecting 1:50 is optimum charging ratio.
Claims (5)
1. a stalk pre-processes microbial bacterial agent with ight soil biogas fermentation, it is characterised in that collectively constituted by following bacterial classification: Penicillum glaucum (Penicillium
glaucum) 2 ~ 8 × 1010Cfu/g, Rhizopus oryzae (Rhizopus
oryzae) 2 ~ 8 × 1010Cfu/g, Phanerochaete chrysosporium (Phanerochaete
chrysosporium) 2 ~ 8 × 1010Cfu/g, aspergillus flavus (Aspergillus
flavus) 2 ~ 8 × 1010Cfu/g, spiral shell spore bacterium (Spirillospora) 2 ~ 8 × 1010cfu/g。
2. the stalk described in claim 1 and ight soil biogas fermentation pre-process the fermentation process of microbial bacterial agent, and its feature is specific as follows:
1) glucose is dissolved in water, adds microbial inoculum and fully stir evenly, microbial inoculum is melted, then dilution is uniformly splashed on stalk, and fully stir uniformly；
2) stalk after step 1) being processed puts in fermentation tank, adds ight soil, carries out the producing methane through anaerobic fermentation of stalk and ight soil；
Wherein, the part by weight composition of material: ight soil 20% ~ 25%, stalk 1% ~ 2%, glucose 0.01% ~ 0.02%, microbial bacterial agent 0.01% ~ 0.02%, running water 70% ~ 80%；Material total moisture should control 90 ~ 92%, and pH is in neutral or alkalescent, and material particular diameter is at 5 ~ 20mm.
Stalk the most according to claim 2 pre-processes the fermentation process of microbial bacterial agent with ight soil biogas fermentation, it is characterised in that: by regulating the pH value of material with soda lime or acetic acid.
Stalk the most according to claim 2 pre-processes the fermentation process of microbial bacterial agent with ight soil biogas fermentation, it is characterised in that: fermentation temperature is 37 DEG C, and every day stirs 1-2 time.
Stalk the most according to claim 2 pre-processes the fermentation process of microbial bacterial agent with ight soil biogas fermentation, it is characterised in that: microbial bacterial agent is 1:50 with the mass ratio of stalk.
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| CN105543287A (en) * | 2016-02-24 | 2016-05-04 | 齐芳 | Treatment method for preparing methane from brewing wastewater |
| CN109328514B (en) * | 2018-10-23 | 2021-11-30 | 湖南杂交水稻研究中心 | Saline-alkali soil improvement method and application thereof |
| CN110002706B (en) * | 2019-04-23 | 2022-08-16 | 上海舒圣投资管理有限公司 | Harmless treatment method for excrement of pig farm |
| CN113088464A (en) * | 2021-03-26 | 2021-07-09 | 张掖兰标生物科技有限公司 | Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof |
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| CN102008011A (en) * | 2010-11-16 | 2011-04-13 | 马德顺 | Straw degradation process |
| CN102173507A (en) * | 2011-01-12 | 2011-09-07 | 南京加德绿色能源研发有限公司 | Method for comprehensively recycling fecal sewage of large-scale livestock and poultry farms and farmland crop straws |
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Patent Citations (3)
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|---|---|---|---|---|
| CN102008011A (en) * | 2010-11-16 | 2011-04-13 | 马德顺 | Straw degradation process |
| CN102173507A (en) * | 2011-01-12 | 2011-09-07 | 南京加德绿色能源研发有限公司 | Method for comprehensively recycling fecal sewage of large-scale livestock and poultry farms and farmland crop straws |
| CN103232944A (en) * | 2013-04-25 | 2013-08-07 | 南京林业大学 | Microorganism bacterium agent for straw and excrement mixed composting |
Non-Patent Citations (1)
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