CN108410772A - A kind of preparation method of straw biological decomposing agent - Google Patents
A kind of preparation method of straw biological decomposing agent Download PDFInfo
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- CN108410772A CN108410772A CN201810308805.0A CN201810308805A CN108410772A CN 108410772 A CN108410772 A CN 108410772A CN 201810308805 A CN201810308805 A CN 201810308805A CN 108410772 A CN108410772 A CN 108410772A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention is directed to decomposed when current straw directly returning to field is decomposed or organic fertilizer is made in compost be not thorough, lead to the present situation that mushroom activity is low, cumbersome, it provides using a kind of to be using fine organic matter as the manufacturing process of culture medium and the fiber hydrolization biological agent of carrier, this method carries out culture activation first with the surging bacterial strain of rejuvenation, passes through viable bacteria biological agent made of anaerobism and oxygen consumption two-step fermentation.Straw decomposing inoculant produced by the present invention is due to there is effective viable bacteria of the decomposing straw ability of compounding (EM bacterium, bacillus subtilis and basidiomycetes); it can speed up the decomposition and softening for promoting cellulose and lignin; the decomposed accumulated temperature of improving material; kill harmful organism; decompose noxious material; improve microbial diversity; promote nutrient validation; make agricultural crop straw Tanaka can quick composting, straw compost also reach completely it is decomposed, it is often more important that Biological straw decomposition agent efficiently uses the ecological environment for protecting the Nature.
Description
Technical field
The invention belongs to microorganism formulation technical field, specially a kind of preparation method of straw biological decomposing agent.
Background technology
Currently, China's stalk is mostly used in returning to the field, improve soil quality, increases crop yield.Due to agricultural crop straw
Mainly cellulose, hemicellulose, lignin composition, it is decomposed slow, it is difficult to decomposed by the Institute of Micro-biology in natural environment, effectively at
Point this season crop utilization is seldom, while having an effect on soil physical property and field farming, thus limits stalk this is important
The cropland application of organic matter resource.Therefore exploring a kind of straw biological decomposing agent makes crop material quick composting, improves stalk also
The operability in field applies farmland after organic fertilizer is made in straw compost, very heavy to promoting the sustainable development of agricultural to have
The realistic meaning wanted.However existing Biological straw decomposition agent majority be using filter mud, manioc waste, bagasse ash as raw material, exist by
It is low that after stalk is added in decomposing agent, microorganism cannot quickly bring back to life, colonize, enzyme producing microbe is lived, and is not easy to keep stalk quickly rotten
Ripe defect, has much room for improvement.
Invention content
The present invention for current straw directly returning to field is decomposed or when organic fertilizer is made in compost it is decomposed be not thorough, lead to mushroom
Low, the cumbersome present situation of activity, the present invention is intended to provide a kind of degradation time is short, decomposition is stablized, and disease pest is effectively killed,
The preparation method of straw biological decomposing agent with extremely strong decomposition.
For this purpose, the present invention uses following technical scheme:A kind of preparation method of anaerobic fermentation decomposing agent, characterized in that packet
Include following preparation process:
Step 1, by rice bran that Soybean Meal that water content is 10%, water content are 10%, water content be 10% fish meal,
Molasses that strain that water content is 10%, water content are 3% play ferment agent and after water mixes and be sufficiently stirred, are put into closed container
It is interior, start anaerobic fermentation;
Step 2 is added to EM bacterium when fermented material water content is 30%, and it is 4.5 to make the pH value of fermented material, conductance
Rate value is 5.5, and organic acid content reaches 2mM, and lactic acid bacterium number is more than every gram 10,000,000.
As supplement to above-mentioned technical proposal and perfect, the invention also includes following technical characteristics.
The EM bacterium are lactic acid bacteria class, budding fungus, the composite bacteria in anaerobic type actinomyces and photosynthetic bacteria.
A kind of preparation method of oxygen consumption fermentation maturity agent, characterized in that including following preparation process:
Leaf, withered grass, corn, rice is added as raw material in grinds after the empty grain baking sieved using wheat and corn processing
Grass crushing straw be used as aerobic bacteria carrier, leaf, withered grass, corn, straw weight be not less than total weight 1/3, addition matter
The anaerobic fermentation decomposing agent that accounting is 30% is measured, EM bacterium, bacillus subtilis and basidiomycetes fermenting microbe are then accessed, by stirring
Mix or lead to oxygen carry out oxygen consumption fermentation, make water content keep 45% or so, when water content is down to 15% or so, detection it is decomposed
Agent PH is 5.8-6.0, is finally fabricated to straw biological decomposing agent.
As supplement to above-mentioned technical proposal and perfect, the invention also includes following technical characteristics.
The EM bacterium are lactic acid bacteria class, budding fungus, the composite bacteria in anaerobic type actinomyces and photosynthetic bacteria.
Fermentation temperature ferments at 60-70 DEG C, after 7 days to be finished.
Fermentation temperature is room temperature, and fermentation time was at 2-3 months.
The present invention can reach following advantageous effect:The present invention carries out culture activation using the surging bacterial strain of rejuvenation, passes through
Viable bacteria biological agent made of anaerobism and oxygen consumption two-step fermentation.Straw decomposing inoculant produced by the present invention is due to there is the decomposition straw of compounding
Effective viable bacteria (EM bacterium, bacillus subtilis and basidiomycetes) of stalk ability can speed up the decomposition for promoting cellulose and lignin
And softening, the decomposed accumulated temperature of improving material kill harmful organism, decompose noxious material, improve microbial diversity, and nutrient is promoted to have
Effectization, make agricultural crop straw Tanaka can quick composting, straw compost also reach completely decomposed, it is often more important that biological straw is rotten
Ripe dose efficiently uses the ecological environment for protecting the Nature.
Specific implementation mode
Embodiment one:The preparation of anaerobic fermentation decomposing agent:A kind of EM composite bacterias are selected, more than 160 kinds of eubacteria is contained, it is main
The four big floras wanted have:Lactic acid bacteria class, budding fungus, anaerobic type actinomyces and photosynthetic bacteria.Anaerobic digestion can
The content for improving anaerobe can retain all nutritional ingredients, kill harmful substance.
Composition of raw materials is as follows:
300kg Soybean Meaies (water content 10%), 370kg rice brans (water content 10%), 100kg fish meal (water content 10%),
10kg strains (water content 10%), 10kg molasses (water content 3%), 1kg play ferment agent and 209kg water, and 1000 kilograms of total material contains
Water reaches 30%, is sufficiently stirred above-mentioned all mixing materials, is put into closed container, starts anaerobic fermentation.If using small
Scale plastic barrel, it is necessary to fully closed.
When sealed fermenting material moisture content is 30%, to pH value, conductivity value, lactic acid content and lactic acid bacteria number
The influence of amount is advantageous.When water content reaches 30%, be added to EM composite bacterias, pH value can be down to 4.5, conductivity value can on
To 5.5, organic acid content reaches 2mM, and lactic acid bacterium number is considerably beyond every gram 10,000,000.Only under this condition with lactic acid bacteria
Based on probiotics can be with mass propagation.
Embodiment two:The preparation of oxygen consumption fermentation maturity agent:Select one kind can decomposition of cellulose actinomyces (EM bacterium) and divide
Lignin basidiomycetes and Bacillus subtilis strain are solved, high fever, quick composting stalk are generated under aerobic condition.
Composition of raw materials is as follows:It is (similar organic as raw material using wheat and grinds after the empty grain baking that corn processing sieves
Matter provides the sources C), the crushing straws such as appropriate leaf, withered grass, corn, straw are added as aerobic bacteria carrier, weight is not less than total
The 1/3 of weight, since stalk carbon-nitrogen ratio is more than 25:1, but aerobic fermentation needs carbon-nitrogen ratio 25:1, ferment effect is best, need to add
Enter 30% anaerobic fermentation decomposing agent (manufactured anaerobic fermentation decomposing agent in (1) above), to provide the sources N of aerobic fermentation, then
EM bacterium, bacillus subtilis and basidiomycetes fermenting microbe are accessed, oxygen consumption fermentation is carried out by stirring or leading to oxygen, makes water content
Keep 45% or so, temperature at 60-70 DEG C, protease, high fever cellulase and amylase competence exertion enzyme superpower effect, it is husky
Men Shi bacillus has inactivated, and ferments 2 day time, it may appear that a large amount of mycelia ferment after 7 days and finish, if room temperature, fermentation time exists
2-3 months or so, when water content is down to 15% or so, the decomposing agent PH of detection was 5.8-6.0, is finally fabricated to straw biological
Decomposing agent, 20--30 days in advance more decomposed than other room temperature of stalk completely decomposed time.
The present invention is with the applicable field of straw biological decomposing agent:
(1) straw biological decomposing agent produced by the present invention can access straw decomposing inoculant in stalk, promote Straw decomposing,
It applies in soil, improves ground temperature, release gas has carbon dioxide fertilization effect in greenhouse.
(2) straw biological decomposing agent produced by the present invention can also make stalk be directly applied for soil as fertilizer application,
The thick miscellaneous organic matter in soil is decomposed, it is interior containing a large amount of bio-bacterial manure, it improves diversity of soil microorganism and increases having for soil
Machine matter content, it is good organic fertilizer to prevent disease, final product.
(3) straw biological decomposing agent can be used as compost fermentation introduction and waste reduction agent, decomposes rubbish and eliminates peculiar smell,
Sludge underwater distintegrant be can be also used for purifying contaminated waters.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (6)
1. a kind of preparation method of anaerobic fermentation decomposing agent, it is characterised in that including following preparation process:
Step 1, the fish meal, aqueous for being 10% by rice bran that Soybean Meal that water content is 10%, water content are 10%, water content
Amount for 10% strain, water content be 3% molasses, rise after ferment agent and water mixes and be sufficiently stirred, be put into closed container, open
Beginning anaerobic fermentation;
Step 2 is added to EM bacterium when fermented material water content is 30%, and it is 4.5 to make the pH value of fermented material, conductivity value
It is 5.5, organic acid content reaches 2mM, and lactic acid bacterium number is more than every gram 10,000,000.
2. a kind of preparation method of anaerobic fermentation decomposing agent according to claim 1, it is characterised in that:The EM bacterium are
Lactic acid bacteria class, budding fungus, the composite bacteria in anaerobic type actinomyces and photosynthetic bacteria.
3. a kind of preparation method of oxygen consumption fermentation maturity agent, it is characterised in that including following preparation process:
Leaf, withered grass, corn, straw is added as raw material in grinds after the empty grain baking sieved using wheat and corn processing
Crushing straw as aerobic bacteria carrier, leaf, withered grass, corn, straw weight be not less than the 1/3 of total weight, quality is added and accounts for
Than the anaerobic fermentation decomposing agent as described in claim 1 for 30%, EM bacterium, bacillus subtilis and basidiomycetes hair are then accessed
Yeast-like fungi kind carries out oxygen consumption fermentation by stirring or leading to oxygen, so that water content is kept 45% or so, 15% or so is down in water content
When, the decomposing agent PH of detection is 5.8-6.0, is finally fabricated to straw biological decomposing agent.
4. a kind of preparation method of oxygen consumption fermentation maturity agent according to claim 3, it is characterised in that:The EM bacterium are
Lactic acid bacteria class, budding fungus, the composite bacteria in anaerobic type actinomyces and photosynthetic bacteria.
5. a kind of preparation method of oxygen consumption fermentation maturity agent according to claim 4, it is characterised in that:Fermentation temperature exists
It ferments and finishes at 60-70 DEG C, after 7 days.
6. a kind of preparation method of oxygen consumption fermentation maturity agent according to claim 4, it is characterised in that:Fermentation temperature is normal
Temperature, fermentation time was at 2-3 months.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109168393A (en) * | 2018-09-26 | 2019-01-11 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | A method of in being applicable in, heavy salinized improve |
CN109777750A (en) * | 2019-01-18 | 2019-05-21 | 黑龙江雄微生物科技有限公司 | A kind of complex microorganism straw decomposing inoculant and preparation method thereof |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109168393A (en) * | 2018-09-26 | 2019-01-11 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | A method of in being applicable in, heavy salinized improve |
CN109777750A (en) * | 2019-01-18 | 2019-05-21 | 黑龙江雄微生物科技有限公司 | A kind of complex microorganism straw decomposing inoculant and preparation method thereof |
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