AU2020100160A4 - Composite microbial agent, biological organic fertilizer, and preparation method and use thereof - Google Patents
Composite microbial agent, biological organic fertilizer, and preparation method and use thereof Download PDFInfo
- Publication number
- AU2020100160A4 AU2020100160A4 AU2020100160A AU2020100160A AU2020100160A4 AU 2020100160 A4 AU2020100160 A4 AU 2020100160A4 AU 2020100160 A AU2020100160 A AU 2020100160A AU 2020100160 A AU2020100160 A AU 2020100160A AU 2020100160 A4 AU2020100160 A4 AU 2020100160A4
- Authority
- AU
- Australia
- Prior art keywords
- parts
- livestock
- fermentation
- poultry manure
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
- Treatment Of Sludge (AREA)
Abstract
The present invention provides a composite microbial agent, a biological organic fertilizer, and a preparation method and use thereof, and belongs to the technical field of microbial agents and organic fertilizers. The composite microbial agent of the present invention includes the following components in parts by mass: 1.5-2.5 parts of Bacillus amyloliquefaciens, 0.5-1 parts of Bacillus lichenformis, 3-4 parts of Bacillus subtilis, 1.5-2 parts of Bacillus megaterium, and 1.5-2.5 parts of Aspergillus oryzae. The composite microbial agent provided by the present invention can enable rapid fermentation of livestock and poultry manure for a required fermentation time of only 10-15 days; also has a good deodorization effect; and enables rapid decomposition of crude fibers in the livestock and poultry manure and effective removal of heavy metals from the livestock and poultry manure.
Description
COMPOSITE MICROBIAL AGENT, BIOLOGICAL ORGANIC FERTILIZER, AND PREPARATION METHOD AND USE THEREOF
TECHNICAL FIELD
The present invention relates to the technical field of microbial agents and organic fertilizers, and in particular to a composite microbial agent, a biological organic fertilizer, and a preparation method and use thereof.
BACKGROUD
At present, the treatment of livestock and poultry manure mostly conducts natural fermentation by adopting original-ecological microbial activating bacteria existing in the nature, and the fermentation time varies greatly according to seasons, where in winter the natural fermentation generally takes 90-120 days or more, and it takes 60-80 days even if heat preservation measures such as plastics are adopted, and in summer it takes mostly 45-50 days. Due to the single variety and insufficient activity of activating strains contained in it, the existing microbial fermentation agent causes a long fermentation time and incomplete treatment of the manure, leading to secondary fermentation after the fermented manure is applied to a farmland, which damages the roots and sprouts of a crop to cause crop death.
Therefore, it is an urgent problem to conduct selective breeding of a microbial fermentation agent for rapid fermentation of livestock and poultry manure.
SUMMARY
In view of this, it would be advantageous if at least preferred embodiments of the present invention to provide a composite microbial agent, a biological organic fertilizer, and a preparation method and use thereof. The composite microbial agent provided by the present invention can enable rapid fermentation of livestock and poultry manure for a required fermentation time of only 10-15 days.
The present invention therefore provides the following technical solutions.
The present invention provides a composite microbial agent, which includes the following components in parts by mass: 1.5-2.5 parts of Bacillus amyloliquefaciens, 0.5-1 parts of Bacillus licheniformis, 3-4 parts of Bacillus subtilis, 1.5-2 parts of Bacillus megaterium, and 1.5-2.5 parts of Aspergillus oryzae.
Preferably, the living bacteria count of the Bacillus amyloliquefaciens is > 2 x 109cfu/g;
2020100160 30 Jan 2020 the living bacteria count of the Bacillus licheniformis is > 0.5 x 109 cfu/g;
the living bacteria count of the Bacillus subtilis is > 1 x 109 cfu/g;
the living bacteria count of the Bacillus megaterium is > 2 x 109cfu/g;
the living spore number of the Aspergillus oryzae is > 10 x 109 cfu/g.
The present invention also provides a fermented product of livestock and poultry manure, which is prepared from the following raw materials in parts by mass: 0.15-0.35 parts of the composite microbial agent of the aforementioned technical solution, 6-19.85 parts of a compostable waste and 79.8-93.85 parts of dehydrated livestock and poultry manure.
Preferably, the compostable waste includes one or more of rice husk, straw and wheat bran.
The present invention also provides a method for preparing the fermented product of livestock and poultry manure of the aforementioned technical solution, including the following steps:
(1) dehydrating livestock and poultry manure to obtain dehydrated livestock and poultry manure with a water content of 55%-60%;
(2) mixing a compostable waste, a composite microbial agent and the dehydrated livestock and poultry manure obtained in the step (1), and then subjecting the mixture to a first fermentation to obtain a first fermented product, wherein the temperature for the first fermentation is 50°C-70°C; and (3) subjecting the fermented product obtained from the step (2) to a second post-aging fermentation to obtain a fermented product of the livestock and poultry manure, wherein the content of organic matters in the fermented product of the livestock and poultry manure is 25%-30%.
The present invention also provides a biological organic fertilizer, which is prepared by mixing 0.1-0.3 parts of the composite microbial agent of the aforementioned technical solution, 74.7-84.9 parts of the fermented product of the livestock and poultry manure, and 15-25 parts of a humic acid.
The present invention provides the use of the composite microbial agent in deodorization of livestock and poultry manure.
The present invention provides the use of the composite microbial agent in decomposing a crude fiber.
The present invention provides the use of the composite microbial agent in degrading a heavy metal.
Preferably, the heavy metal includes one or more of mercury, lead, chromium, cadmium and arsenic.
2020100160 30 Jan 2020
For the composite microbial agent provided by the present invention, the strains contained in it has strong activity, high acid-base tolerance, high capability of dissolving fixed nitrogen, phosphorus and potassium and decomposing organic matters; and there is strong compatibility and no phagocytosis between the strains; this enables rapid fermentation of the livestock and poultry manure for a required fermentation time of only 10-15 days.
DESCRIPTION OF THE EMBODIMENTS
The present invention provides a composite microbial agent, which includes the following components in parts by mass: 1.5-2.5 parts of Bacillus amyloliquefaciens, 0.5-1 parts of Bacillus licheniformis, 3-4 parts of Bacillus subtilis, 1.5-2 parts of Bacillus megaterium, and 1.5-2.5 parts of Aspergillus oryzae.
The composite microbial agent provided by the present invention includes 1.5-2.5 parts by mass, preferably 1.8-2.2 parts by mass, and more preferably 2 parts by mass of Bacillus amyloliquefaciens. The present invention has no specific limitation on the source of the Bacillus amyloliquefaciens, and preferably the Bacillus amyloliquefaciens is screened out from naturally-fermented manure.
In the present invention, the fermentation culture conditions of the Bacillus amyloliquefaciens are not particularly limited, and the following fermentation medium and fermentation conditions are preferably used.
In percentage by mass, the fermentation medium for the Bacillus amyloliquefaciens is preferably composed of 0.2%-0.3% of sucrose, 1.2%-1.8% of soybean meal, 0.008%-0.012% of magnesium chloride, 0.008%-0.012% of manganese sulfate, and the balance of sterile water; and more preferably composed of 0.25% of sucrose, 1.5% of soybean meal, 0.01% of magnesium chloride, 0.01% of manganese sulfate, and 98.23% of sterile water.
The fermentation conditions for the Bacillus amyloliquefaciens preferably include: a fermentation time of preferably 40-60 h, and more preferably 48 h; and a fermentation temperature of preferably 36°C-38°C, and more preferably 37°C.
In the present invention, the living bacteria count of the Bacillus amyloliquefaciens is preferably > 2 x 109 cfu/g, more preferably (2 x 109, 5x 109] cfu/g, and most preferably 3-4 x 109 cfu/g.
In the present invention, the Bacillus amyloliquefaciens has an antifungal protein, a surfactin and a chitinase, and the generated dissolved substance can digest a mycelium and a cell wall, so that the thallus and the organic matters are broken, disintegrated, subjected to cell wall perforation and deformed, so as to play a dissolving role.
2020100160 30 Jan 2020
The composite microbial agent provided by the present invention includes 0.5-1 parts by mass, preferably 0.6-0.9 parts by mass, and more preferably 0.8 parts by mass of Bacillus licheniformis. The present invention has no specific limitation on the source of the Bacillus licheniformis, and preferably the Bacillus licheniformis is screened out from soil.
The present invention has no specific limitation on the fermentation culture conditions of the Bacillus licheniformis, and preferably the following fermentation culture medium and fermentation conditions are adopted:
In percentage by mass, the fermentation medium of the Bacillus licheniformis is preferably composed of 1.2%-1.7% of glucose, 0.8%-1.2% of a yeast extract, 0.28%-0.32% of magnesium sulfate, and the balance of sterile water; and more preferably composed of 1.5% of glucose, 1.0% of a yeast extract, 0.3% of magnesium sulfate, and 97.2% of sterile water.
The fermentation temperature for the Bacillus licheniformis is preferably 36°C-38°C, and more preferably 37°C.
In the present invention, the living bacteria count of the Bacillus licheniformis is preferably > 0.5 x 109 cfu/g, more preferably (0.5 x 109, 2 x 109] cfu/g, and most preferably 1-1.5 x 109 cfu/g.
In the present invention, the Bacillus licheniformis can adjust dysbacteriosis, promote an organism to produce antibacterial active substances and kill pathogenic bacteria; and it also has a unique biological oxygen scavenging action mechanism, and can inhibit the growth and reproduction of pathogenic bacteria.
The composite microbial agent provided by the present invention includes 3-4 parts by mass, preferably 3.2-3.8 parts by mass, and more preferably 3.5 parts by mass of Bacillus subtilis. The present invention has no specific limitation on the source of the Bacillus subtilis, and preferably the Bacillus subtilis is selected from soil or decaying organic matters.
The present invention has no specific limitation on the fermentation culture conditions of the Bacillus subtilis of the present invention, and preferably the following fermentation culture media and fermentation conditions are adopted.
In percentage by mass, the fermentation medium for the Bacillus subtilis is preferably composed of 1.8-2% glucose, 1.4-1.5% of peptone, 0.48-0.52% of sodium chloride, 0.048-0.052% of beef extract, 1.8-2% of agar, and the balance of sterile water; and more preferably composed of 1.9% of glucose, 1.45% of peptone, 0.5% of sodium chloride, 0.05% of beef extract, 1.9% of agar, and 94.2% of sterile water.
The fermentation conditions adopted for culturing Bacillus subtilis preferably include: a fermentation time of preferably 15-25 h, and more preferably 20 h; and a fermentation
2020100160 30 Jan 2020 temperature of preferably 35°C-38°C, and more preferably 36.5°C.
In the present invention, the living bacteria count of the Bacillus subtilis is preferably > 1 x 109 cfu/g, more preferably (1 x 109, 2 x 109] cfu/g, and most preferably 1.5 x 109 cfu/g.
In the present invention, the Bacillus subtilis can generate active substances such as subtilisin, Polymyxin, Nystatin, Gramicidin and the like in the growth process, thereby accelerating the decomposition of organic substances.
The composite microbial agent provided by the present invention includes 1.5-2 parts by mass, preferably 1.6-1.9 parts by mass, and more preferably 1.8 parts by mass of Bacillus megaterium. The present invention has no specific limitation on the source of the Bacillus megaterium, and preferably the Bacillus megaterium is screened out from naturally-fermented manure.
In the present invention, the fermentation culture conditions of the Bacillus megaterium are not particularly limited, and the following fermentation medium and fermentation conditions are preferably used.
In percentage by mass, the fermentation medium for the Bacillus megaterium is preferably composed of 0.8%-1.2% of corn flour, 0.8%-1.2% of soybean peptone, 0.08%-0.12% of calcium chloride dihydrate, 0.045%-0.055% of manganese sulfate, and the balance of sterile water; and more preferably composed of 1% of com flour , 1% of soybean peptone, 0.1% of calcium chloride dihydrate, 0.05% of manganese sulfate, and 97.85% of sterile water.
The fermentation conditions for the Bacillus megaterium preferably include: a fermentation time of preferably 40-60 h, and more preferably 48 h; and a fermentation temperature of preferably 28°C-32°C, and more preferably 30°C.
In the present invention, the living bacteria count of the Bacillus megaterium is preferably > 2 x 109 cfu/g, more preferably (2 x 109, 2.5 x 109] cfu/g, and most preferably 2.25 x 109 cfu/g.
In the present invention, the Bacillus megaterium has the characteristics of rapid propagation, strong vitality, safety, nontoxicity and the like; and can eliminate malodorous gases generated by decomposition of human, livestock and poultry manure. The secreted organic acid substances can dissolve calcium phosphate, iron phosphate, aluminum phosphate and the like compounds which are not easily absorbed by a crop from the soil, facilitating the dissolution and utilization of ineffective phosphorus in the soil.
The composite microbial agent provided by the present invention includes 1.5-2.5 parts by mass, preferably 1.6-2.4 parts by mass, and more preferably 2 parts by mass of Aspergillus oryzae. The present invention has no specific limitation on the source of the Aspergillus oryzae, and preferably the Aspergillus oryzae is screened out from a fermented product of domestic
2020100160 30 Jan 2020 wastes.
The present invention has no specific limitation on the fermentation culture conditions of the Aspergillus oryzae, and the following fermentation medium is preferably used.
In percentage by mass, the fermentation medium for Aspergillus oryzae is preferably composed of 30%-35% of soybean meal, 30% of bran, and the balance of sterile water.
In the present invention, the living spore number of Aspergillus oryzae is preferably > 10 x 109 cfu/g, more preferably (10 x 109, 15 x 109] cfu/g, and most preferably 12-13 x 109 cfu/g.
In the present invention, the Aspergillus oryzae can also produce a amylase, a Diastase, a Cellulase, a Phytase and the like in addition to Complex enzyme; can rapidly and efficiently decompose various organic matters (proteins, cellulose, hemicellulose, lignin and the like) in organic wastes, and has the functions of warming up, deodorizing, eliminating plant diseases and insect pests, weed seeds, and improving nutrients in the biological fermentation process.
The present invention has no specific limitation on the method for preparing the composite microbial agent, and preferably the preparation method includes the following steps:
(1) respectively activating the Bacillus amyloliquefaciens, the Bacillus licheniformis, the Bacillus subtilis, the Bacillus megaterium and the Aspergillus oryzae to respectively obtain activated strains;
(2) mixing and culturing the activated strains respectively obtained in the step (1) each with a seed solution to respectively obtain first-stage seed solutions;
(3) mixing the first-stage seed solutions respectively obtained in the step (2) each with a fermentation culture medium, then respectively subjecting to a first fermentation to respectively obtain first-stage fermentation liquors;
(4) subjecting the first-stage fermentation liquors respectively obtained in the step (3) to a second fermentation to respectively obtain second-stage fermentation liquors;
(5) subjecting the second-stage fermentation liquors respectively obtained in the step (4) to a third fermentation to respectively obtain third-stage fermentation liquors; and (6) mixing the third-stage fermentation liquors respectively obtained in the step (5) to obtain the composite microbial agent.
In the present invention, the Bacillus amyloliquefaciens, the Bacillus licheniformis, the Bacillus subtilis, the Bacillus megaterium and the Aspergillus oryzae are respectively activated to respectively obtain activated strains. The present invention has no specific limitation on the method for activating the strains, and a method conventionally used for activating the aforementioned strains can be adopted.
In the present invention, the respectively obtained activated strains are mixed with a seed
2020100160 30 Jan 2020 solution and then cultured to obtain first-stage seed solutions respectively; The present invention has no specific limitation on the method for culturing the seed solutions, and preferably the seed solutions are subjected to shaking culture; and the rotation speed of the shaking culture is preferably 180-220 r/min, and more preferably 200 r/min.
In the present invention, the respectively obtained first-stage seed solutions are each mixed with a fermentation culture medium, and then respectively subjected to a first fermentation to respectively obtain first-stage fermentation liquors. The present invention has no specific limitation on the method for the first fermentation culture, and preferably the first-stage seed solutions are inoculated into a 30 L first-stage fermentation tank at an inoculation amount of 5%-10% for culture; and the culture conditions are preferably a ventilation ratio of 1:1, a rotation speed of 100 r/min, 37°C, and a culture time of 8-12 h.
In the present invention, the respectively obtained first-stage fermentation liquors are subjected to a second fermentation to respectively obtain second-stage fermentation liquors. The present invention has no specific limitation on the method for second-stage fermentation culture, and preferably the first-stage fermentation liquors are inoculated into a 300 L second-stage fermentation tank for culture; and the culture time is preferably 15-18 h.
In the present invention, the respectively obtained second-stage fermentation liquors are subjected to a third fermentation to obtain third-stage fermentation liquors respectively. The present invention has no specific limitation on the method for the third-stage fermentation culture, and preferably the second-stage fermentation liquors are inoculated into a 3,000 L third-stage fermentation tank for culture; and the culture time is preferably 20-24 h.
In the present invention, the respectively obtained third-stage single strain fermentation liquors are mixed to obtain the composite microbial agent.
In the present invention, before mixing the respectively obtained third-stage bacteria solution, it is preferable to use a dilute spread-plate method to calculate the number of viable bacteria, which is served as a blending base value of the microbial agent compounding amount or used to determine the value of fermentation viable bacteria amount, so as to determine whether to continue to prolong the fermentation time or complete the fermentation, thereby achieving a target value.
In the present invention, for counting the number of viable bacteria through the dilute spread-plate method, preferably 1 g of a bacteria solution is taken and subjected to a conventional dilute spread-plate method to count the number of viable bacteria.
The present invention also provides a fermented product of livestock and poultry manure, which is prepared from the following raw materials in parts by mass: 0.15-0.35 parts of the
2020100160 30 Jan 2020 composite microbial agent of the aforementioned technical solution, 6-19.85 parts of a compostable waste and 79.8-93.85 parts of dehydrated livestock and poultry manure.
The fermented product of livestock and poultry manure as provided by the present invention includes 0.15-0.35 parts by mass, and preferably 0.2 parts by mass of the composite microbial agent.
The fermented product of livestock and poultry manure as provided by the present invention includes 6-19.85 parts by mass, preferably 8-19 parts by mass, and more preferably 15 parts by mass of a compostable waste. The present invention has no specific limitation on the types of the compostable waste, and preferably the compostable waste is one or more of rice husk, straw and wheat bran; and when the compostable waste is preferably two or more of the aforementioned substances, they are preferably mixed in an equal mass ratio.
The present invention also provides a method for preparing the fermented product of livestock and poultry manure of the aforementioned technical solution, including the following steps:
(1) dehydrating livestock and poultry manure to obtain dehydrated livestock and poultry manure with a water content of 55%-60%;
(2) mixing a compostable waste, a composite microbial agent and the dehydrated livestock and poultry manure obtained in the step (1), and then subjecting the mixture to a first fermentation to obtain a first fermented product, wherein the temperature for the first fermentation is 50°C-70°C; and (3) subjecting the fermented product obtained from the step (2) to a second post-aging fermentation to obtain a fermented product of the livestock and poultry manure, wherein the content of organic matters in the fermented product of the livestock and poultry manure is 25%-30%.
In the present invention, the livestock and poultry manure is dehydrated to obtain dehydrated livestock and poultry manure with a water content of 55%-60%. The present invention has no specific limitation on the method for dehydrating the livestock and poultry manure, and preferably a crawler-type dry-wet separator is adopted for dehydration.
In the present invention, the compostable waste, the composite microbial agent and the obtained dehydrated livestock and poultry manure are mixed and then subjected to a first fermentation to obtain a first fermented product; and the temperature for the first fermentation is 50°C-70°C.
In the present invention, when the fermentation temperature reaches 55°C, it is preferable to conduct turning once a day; when the temperature reaches greater than 65°C, it is preferable to
2020100160 30 Jan 2020 conduct turning twice a day; and when the temperature falls below 45°C, the turning is stopped. The present invention has no specific limitation on the turning manner, and preferably a chain-and-slat type fertilizer turner is adopted for turning.
In the present invention, the obtained first fermented product is subjected to a second post-aging fermentation to obtain a fermented product of the livestock and poultry manure, where the content of organic matters in the fermented product of the livestock and poultry manure is 25%-30%. The present invention has no specific limitation on the aging method, and conventional natural stacked aging can be adopted.
The present invention also provides a biological organic fertilizer, which is prepared by mixing 0.1-0.3 parts of the composite microbial agent of the aforementioned technical solution, 74.7-84.9 parts of the fermented product of the livestock and poultry manure, and 15-25 parts of a humic acid.
The biological organic fertilizer provided by the present invention includes 15-25 parts by mass, preferably 18-24 parts by mass, and more preferably 21 parts by mass of a humic acid. The present invention has no specific limitation on the source of the humic acid, and preferably the humic acid has an organic matter content of 75%-85%.
In the present invention, the obtained fermented product of livestock and poultry manure is mixed with the humic acid and the composite microbial agent to obtain the biological organic fertilizer. The present invention has no specific limitation on the mixing manner, and a conventional material mixing method can be adopted.
The present invention provides the use of the composite microbial agent in deodorization of livestock and poultry manure.
The present invention provides the use of the composite microbial agent in decomposing a crude fiber.
The present invention provides the use of the composite microbial agent in degrading a heavy metal.
In the present invention, the heavy metal is preferably one or more of mercury, chromium, cadmium, lead and arsenic.
The technical solutions in the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are merely a part rather than all of the embodiments of the present invention. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.
2020100160 30 Jan 2020
Example 1
A composite microbial agent was prepared by compounding 20% of Bacillus amyloliquefaciens, 10% of Bacillus licheniformis, 35% of Bacillus subtilis, 20% of Bacillus megaterium and 15% of Aspergillus oryzae in percentage by mass. The living bacteria count of the Bacillus amyloliquefaciens was 2 x 109 cfu/g; the living bacteria count of the Bacillus licheniformis is 0.5 x 109 cfu/g; the living bacteria count of the Bacillus subtilis is 1 x 109 cfu/g; the living bacteria count of the Bacillus megaterium is 2 x 109 cfu/g; and the living spore number of the Aspergillus oryzae is 10 x 109 cfu/g.
Example 2
A composite microbial agent was prepared by compounding 20% of Bacillus amyloliquefaciens, 10% of Bacillus licheniformis, 35% of Bacillus subtilis, 20% of Bacillus megaterium and 15% of Aspergillus oryzae in percentage by mass. The living bacteria count of the Bacillus amyloliquefaciens was 5 x 109 cfu/g; the living bacteria count of the Bacillus licheniformis is 2 x 109 cfu/g; the living bacteria count of the Bacillus subtilis is 2 x 109 cfu/g; the living bacteria count of the Bacillus megaterium is 2.5 x 109 cfu/g; and the living spore number of the Aspergillus oryzae is 15 x 109 cfu/g.
Example 3 kg of pig manure, 52 kg of chicken manure and 131 kg of cow manure were mixed and then dehydrated by a crawler-type extrusion dry-wet separator. When the water content was reduced from 90% to 55%, 91.7 kg of mixed manure was obtained, it was added with 8 kg of crushed straw, and then uniformly added with 0.3 kg of the composite microbial agent prepared in Example 1 for inoculation (for the water content of the manure raw material, and the mixing proportion of it to other raw materials, the total C: N content was 1:30). The manure inoculated with the composite microbial agent was introduced into a fermentation tank by a forklift for fermentation. On the 3rd day after being introduced into the fermentation tank, monitoring and checking of the temperature change situation was started. When the temperature reached 55°C, turning was conducted with a chain-and-slat type fertilizer turner once a day; when the temperature reached greater than 65°C, turning was conducted twice a day; and when the temperature fallen below 45°C, the turning was stopped. This process taken 10 days in summer and 12 days in winter. After completion of the high-temperature fermentation stage, the content was taken out from the fermentation tank by a forklift for a secondary post-aging fermentation, where the manure fermentation stage was completed after 30 days of natural stacking. The obtained product was the fermented product of livestock and poultry manure, which was the basic raw material of a biological organic fertilizer.
2020100160 30 Jan 2020
Example 4
The fermented product of livestock and poultry manure obtained in Example 3, as the basic raw material of the biological organic fertilizer, was subjected to detection for an organic matter content. The organic matter content of the fermented manure raw material was 28%, the organic matter content of the humic acid was 85%, and the mass ratio of the fermented product of livestock and poultry manure, the humic acid and the composite microbial agent prepared in Example 2 was 78.5:21.3:0.2. The above materials were fully mixed to obtain a biological organic fertilizer (powder).
Example 5
The powder biological organic fertilizer obtained in Example 4 was granulated by a granulator to obtain a granular biological organic fertilizer.
According to Example 3 of the present invention, it can be seen that the composite microbial agent provided by the present invention enables rapid fermentation of the livestock and poultry manure for a required fermentation time of 10 days in summer and 12 days in winter.
The above description is only preferred embodiments of the present invention. It should be pointed out that, for those of ordinary skills in the art, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be considered as falling into the claimed scope of the present invention.
In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word “comprise” or variations such as “comprises” or “comprising” is used in an inclusive sense, i.e. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.
Claims (5)
- (1) dehydrating livestock and poultry manure to obtain dehydrated livestock and poultry manure with a water content of 55%-60%;1. A composite microbial agent, comprising the following components in parts by mass: 1.5-2.5 parts of Bacillus amyloliquefaciens, 0.5-1 parts of Bacillus licheniformis, 3-4 parts of Bacillus subtilis, 1.5-2 parts of Bacillus megaterium, and 1.5-2.5 parts of Aspergillus oryzae.
- (2) mixing a compostable waste, a composite microbial agent and the dehydrated livestock and poultry manure obtained in the step (1), and then subjecting the mixture to a first fermentation to obtain a first fermented product, wherein the temperature for the first fermentation is 50°C-70°C; and (3) subjecting the first fermented product obtained from the step (2) to a second post-aging fermentation to obtain a fermented product of the livestock and poultry manure, wherein the content of organic matters in the fermented product of the livestock and poultry manure is 25%-30%.2. The composite microbial agent according to claim 1, wherein the living bacteria count of the Bacillus amyloliquefaciens is > 2 x 109 cfu/g;the living bacteria count of the Bacillus licheniformis is > 0.5 x 109 cfu/g;the living bacteria count of the Bacillus subtilis is > 1 x 109 cfu/g;the living bacteria count of the Bacillus megaterium is > 2 x 109cfu/g;the living spore number of the Aspergillus oryzae is > 10 x 109 cfu/g.
- 3. A fermented product of livestock and poultry manure, which is prepared from the following raw materials in parts by mass: 0.15-0.35 parts of the composite microbial agent according to claim 1, 6-19.85 parts of a compostable waste and 79.8-93.85 parts of dehydrated livestock and poultry manure, preferably, wherein the compostable waste comprises one or more of rice husk, straw and wheat bran.
- 4. A method for preparing the fermented product of livestock and poultry manure according to claim 3, comprising the following steps:
- 5. A biological organic fertilizer, which is prepared by mixing 74.7-84.9 parts of the fermented product of the livestock and poultry manure according to claim 3, 15-25 parts of a humic acid, and 0.1-0.3 parts of the composite microbial agent according to claim 1.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910269687.1A CN111793570B (en) | 2019-04-04 | 2019-04-04 | Compound microbial agent, bio-organic fertilizer, and preparation method and application thereof |
| CN201910269687.1 | 2019-04-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020100160A4 true AU2020100160A4 (en) | 2020-03-12 |
Family
ID=69724794
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020100160A Ceased AU2020100160A4 (en) | 2019-04-04 | 2020-01-30 | Composite microbial agent, biological organic fertilizer, and preparation method and use thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN111793570B (en) |
| AU (1) | AU2020100160A4 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114275906A (en) * | 2021-12-10 | 2022-04-05 | 安徽安泰农业开发有限责任公司 | Composite microbial inoculum for treating sewage of pig farm and preparation method thereof |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375714B (en) * | 2020-11-26 | 2022-09-13 | 河北一然生物科技股份有限公司 | Composite microecological preparation suitable for low-temperature rapid fermentation of livestock and poultry manure |
| CN112725239B (en) * | 2021-02-05 | 2023-04-21 | 宁夏大学 | Decomposition agent for degrading cellulose and deodorizing function as well as preparation method and application thereof |
| CN113105278A (en) * | 2021-04-12 | 2021-07-13 | 昆明根之力生物科技有限公司 | Agricultural microbial enzyme, biological organic fertilizer and preparation method |
| CN115216415B (en) * | 2021-04-16 | 2023-12-26 | 中国农业大学 | Microbial inoculum and application thereof in preparation of fertilizer |
| CN115215690A (en) * | 2021-04-16 | 2022-10-21 | 中国农业大学 | Livestock manure treatment method, microbial inoculum and application |
| CN113735652A (en) * | 2021-08-27 | 2021-12-03 | 河北昱鼎生物科技有限公司 | Efficient organic material decomposing agent and preparation method and application thereof |
| CN113620752A (en) * | 2021-09-06 | 2021-11-09 | 广东汇先丰农牧有限公司 | Chicken manure organic fertilizer rich in microbial strains and preparation method thereof |
| CN113789282A (en) * | 2021-09-30 | 2021-12-14 | 北京饕餮环保科技有限公司 | High-temperature aerobic composite microbial agent for kitchen waste treatment and preparation method thereof |
| CN113773146A (en) * | 2021-10-19 | 2021-12-10 | 广西壮族自治区农业科学院 | Biological organic fertilizer for Shanghai Qing and preparation method thereof |
| CN114736046A (en) * | 2022-05-18 | 2022-07-12 | 济南新未来生物技术有限公司 | Process method for preparing liquid suspended fertilizer by converting livestock and poultry manure |
| CN115521181A (en) * | 2022-11-09 | 2022-12-27 | 辽宁拓展农业发展有限公司 | Fertilizer containing beneficial bacteria and preparation method thereof |
| CN117209553A (en) * | 2023-07-21 | 2023-12-12 | 江苏海洋大学 | Probiotic agent for improving wall breaking rate of porphyra yezoensis by combined strain fermentation and fermentation method thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005014828A2 (en) * | 2003-08-01 | 2005-02-17 | Basf Plant Science Gmbh | Process for the production of fine chemicals in plants |
| CN101774828B (en) * | 2010-01-28 | 2012-09-05 | 浙江省农业科学院 | Composting method for biologically dehydrating livestock manure |
| CN103058772B (en) * | 2013-01-15 | 2015-07-01 | 内蒙古永业生物技术有限责任公司 | Preparation method for high-activity humic acid biofertilizer |
| CN106493167B (en) * | 2015-09-07 | 2019-06-25 | 粮华生物科技（北京）有限公司 | Bacillus licheniformis and microbial inoculum and their application and heavy metal-passivated method |
| CN106747732A (en) * | 2016-12-19 | 2017-05-31 | 陕西绿科生物科技有限公司 | A kind of bio-feritlizer |
| CN107619799A (en) * | 2017-04-13 | 2018-01-23 | 济南航晨生物科技有限公司 | A kind of agricultural marine alga intermediate extraction specific complex microbial bacterial agent and preparation method thereof |
| CN109516845A (en) * | 2018-12-29 | 2019-03-26 | 湖南润丰达生态环境科技有限公司 | A kind of livestock and poultry feces organic fertilizer fermentation complex function packet and its preparation method and application |
-
2019
- 2019-04-04 CN CN201910269687.1A patent/CN111793570B/en active Active
-
2020
- 2020-01-30 AU AU2020100160A patent/AU2020100160A4/en not_active Ceased
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114275906A (en) * | 2021-12-10 | 2022-04-05 | 安徽安泰农业开发有限责任公司 | Composite microbial inoculum for treating sewage of pig farm and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111793570B (en) | 2022-03-15 |
| CN111793570A (en) | 2020-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020100160A4 (en) | Composite microbial agent, biological organic fertilizer, and preparation method and use thereof | |
| CN101786911B (en) | Microorganism and enzyme powder composite biological agent for microbial organic fertilizer | |
| CN109679860B (en) | Composite microbial inoculum for treating garden green waste and preparation method and application thereof | |
| CN100387551C (en) | Method of producing active nutritional fertilizer using old domostic garbage | |
| CN102924185B (en) | Chicken manure fermented fertilizer | |
| AU2020103489A4 (en) | A straw-decomposing bacterial agent and its application | |
| CN105296394A (en) | Microbial decomposition agent for animal wastes and straws and preparation method thereof | |
| TWI410394B (en) | Fermented Fertilizers Containing Bamboo Active Ingredients and Their Manufacturing Methods | |
| CN104261911A (en) | Method for preparing organic fertilizer by using plant residues as raw material | |
| CN101798241B (en) | Method for producing microbial organic fertilizer by using sludge from sewage plant | |
| CN103694004B (en) | A kind of preparation method of seaweed bio organic fertilizer | |
| CN107522541A (en) | Biological organic fertilizer produced using chicken manure and furfural dregs and preparation method thereof | |
| CN101781153A (en) | Organic fertilizer for killing underground injurious insect and preparation method thereof | |
| CN112759479A (en) | Sheep manure organic biological fertilizer and preparation method thereof | |
| CN104250119A (en) | Method for producing bio-organic fertilizer through fermentation wastewater and agricultural waste | |
| CN108794189A (en) | A kind of preparation method of NEW TYPE OF COMPOSITE bio-feritlizer | |
| CN112028687A (en) | Kitchen waste prepared liquid organic fertilizer and production process thereof | |
| CN103708864A (en) | Biological bacterial fertilizer for saline land crops, and preparation method thereof | |
| CN112457076A (en) | Formula for preparing organic fertilizer and method for preparing organic fertilizer by using same | |
| CN108017428A (en) | A kind of straw decomposition microbial inoculum and its application | |
| CN108410772A (en) | A kind of preparation method of straw biological decomposing agent | |
| CN102786327A (en) | Biological organic fertilizer produced from abamectin fermentation waste residue and preparation method thereof | |
| CN109232131A (en) | A kind of biological environmental production fertilizer | |
| CN104355919A (en) | Botanical insecticidal bio-fertilizer | |
| JP2003055081A (en) | Sludge fermented fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |