CN107619799A - A kind of agricultural marine alga intermediate extraction specific complex microbial bacterial agent and preparation method thereof - Google Patents
A kind of agricultural marine alga intermediate extraction specific complex microbial bacterial agent and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to fertilizer and microbial technology field, and in particular to a kind of agricultural marine alga intermediate extraction specific complex microbial bacterial agent, and the preparation method of the agricultural marine alga intermediate extraction specific complex microbial bacterial agent.Agricultural marine alga intermediate extraction specific complex microbial bacterial agent uses a variety of different types of bacteriums and fungi, and respectively after deep fermentation, processing obtains corresponding bacterium powder.Then bacterium powder is compounded, obtains agricultural marine alga intermediate extraction specific complex microbial bacterial agent.It is the particular approach of the present invention to the addition of complex micro organism fungicide can lift product entirety fertilizer efficiency while agricultural marine alga intermediate recovery rate is improved.
Description
Technical field
The invention belongs to technical field of fertilizers and microbial technology field, and in particular to a kind of agricultural marine alga intermediate extraction
The preparation method of specific complex microbial bacterial agent.
Background technology
Agricultural marine alga intermediate is using the plant rich in marine alga or marine algae extract as raw material, passes through fermentation, soda acid technique
Or the agricultural biological fertilizer that fertilizer compounding process is produced.Agricultural marine alga intermediate has improvement soil physical property, influences root
Border microbial population, promote function, the growths to crops such as crop photosynthesis, raising crop anti-adversity that there is good rush
Enter effect.
It is however, industrial at present generally using chemical reaction flows marine alga intermediates such as acid treatments.It is heavy in its extraction process
Drop and filtering technique are wasted time and energy, and the marine alga extracted is unstable, easy decomposition, and recovery rate and viscosity are generally than relatively low.It is above-mentioned
Factor largely limits should and promoting for marine alga.
Agricultural probiotics is vital movement improvement soil, the microecological balance of plant by microorganism, causes plant to obtain
To a kind of active beneficial microbe of specific benign fertilizer effect.Agricultural probiotics can improve roots of crops Micro Ecosystem,
The insoluble phosphorus of rich content, potassium and trace element in soil are discharged, or the material that crops are difficult by turns
Effective nutritional ingredient is turned to, promotes plant growth.Meanwhile some agricultural probiotics can also reduce the generation of soil-borne disease of crop,
Alleviate fertilizer imbalance between supply and demand, eliminate the adverse effect to environment, played a great role for agricultural sustainable development.
The content of the invention
In order to solve the technical problem of agricultural marine alga intermediate extraction, the invention provides a kind of agricultural marine alga intermediate to carry
The specific complex microbial bacterial agent taken, and preparation method thereof.
The agricultural marine alga intermediate extraction specific complex microbial bacterial agent carries out deep-layer liquid hair respectively by more than ten kind bacterial strains
Ferment, gained zymotic fluid are prepared as bacterium powder respectively, by certain proportion mix agricultural marine alga intermediate extract it is special compound
Microbial bacterial agent.Strain includes cellulase producing strain, production amylase strain, yielding lipase bacterial strain, production nuclease bacterial strain, production fruit
Glue enzyme bacterial strain and bacteria produced proteinase strain.
Above-mentioned cellulase producing strain includes bacillus subtilis, Trichoderma viride, Trichoderma harzianum, trichoderma reesei, Kang Shi wood
One or more of mixtures in mould；Described production amylase strain include bacillus amyloliquefaciens, goose source penicillium oxalicum, tiltedly
One or more of mixtures in sleeping mould, Paecilomyces thermaphila bacterium；Described yielding lipase bacterial strain include bacillus cereus,
One or more of mixtures in bacillus thuringiensis, bacillus subtilis；Described production nuclease bacterial strain includes huge
One or more of mixtures in bacillus, bacillus licheniformis, bacillus pumilus, atrophy bacillus；Described
Produce one or more of mixing in pectase bacterial strain Campinas series bacillus, aspergillus oryzae, aspergillus niger, bacillus polymyxa
Thing；Described bacteria produced proteinase strain includes bacillus subtilis, bacillus megaterium, bacillus laterosporus, bafillus natto
In one or more of mixtures.
Each producing enzyme microbial inoculum proportion is with weight in the special complex micro organism fungicide formula of agricultural marine alga intermediate extraction
Part meter is as follows：
Cellulase-producing microbial inoculum 30-50
Produce amylase microbial inoculum 20-40
Yielding lipase microbial inoculum 10-20
Produce nuclease microbial inoculum 10-20
Produce pectase microbial inoculum 5-10
Bacteria produced proteinase agent 5-10
Preferable each producing enzyme microbial inoculum proportion is as follows in parts by weight：
Cellulase-producing microbial inoculum 35-45
Produce amylase microbial inoculum 25-35
Yielding lipase microbial inoculum 12-18
Produce nuclease microbial inoculum 12-18
Produce pectase microbial inoculum 5-8
Bacteria produced proteinase agent 5-8
Above-mentioned each microbial inoculum preparation technology is as follows：
1）Cellulase producing strain preparation method：
Increase bacterium culture medium formula：Glucose 0.5-1.0%, yeast extract 0.02-0.06%, peptone 0.5-1.0%, NaCl
0.2-0.6%, KH2PO40.2-0.5%, MgSO4·7H2O 0.01-0.06%, sodium carboxymethylcellulose 0.2-0.8 %, tween
80 0.4-0.8%, pH 7.2 ~ 7.4.
Fermentation condition：Ventilation ratio is 1.0-1.5, and 35-40 DEG C of cultivation temperature, speed of agitator 160-200 rpm, culture 24-48 are small
When, for gained zymotic fluid after plate compression, 45-55 DEG C of drying and crushing of bacterial sediment obtains microbial inoculum.
2）Produce amylase strain preparation method：
Increase bacterium culture medium formula：Glucose 0.5-1.0%, yeast extract 0.02-0.06%, peptone 0.5-1.0%, NaCl
0.2-0.6%, KH2PO40.2-0.5%, MgSO4·7H2O 0.01-0.06%, soluble starch 1.0-2.0%, pH 7.2 ~
7.4。
Fermentation condition：Same cellulase producing strain；
3）Yielding lipase bacterial strain preparation method：
Increase bacterium culture medium formula：Glucose 0.5-1.0%, yeast extract 0.02-0.06%, peptone 0.5-1.0%, NaCl
0.2-0.6%, KH2PO40.2-0.5%, MgSO4·7H2O 0.01-0.06%, soybean oil 0.5-1.0%, pH 7.2 ~ 7.4.
Fermentation condition：Same cellulase producing strain；
4）Produce nuclease bacterial strain preparation method：
Increase bacterium culture medium formula：Glucose 0.5-1.0%, yeast extract 0.02-0.06%, peptone 0.5-1.0%, NaCl
0.2-0.6%, KH2PO40.2-0.5%, MgSO4·7H2O 0.01-0.06%, soybean lecithin 0.01-0.05%, pH 7.2 ~
7.4。
Fermentation condition：Same cellulase producing strain；
5）Produce pectase bacterial strain preparation method：
Increase bacterium culture medium formula：Glucose 0.5-1.0%, yeast extract 0.02-0.06%, peptone 0.5-1.0%, NaCl
0.2-0.6%, KH2PO40.2-0.5%, MgSO4·7H2O 0.01-0.06%, pectin 0.1-0.5%, pH 7.2 ~ 7.4.
Fermentation condition：Same cellulase producing strain；
6）Bacteria produced proteinase strain preparation method：
Increase bacterium culture medium formula：Glucose 0.5-1.0%, yeast extract 0.02-0.06%, dregs of beans 1-2%, peptone 0.5-
1.0%, NaCl 0.2-0.6%, KH2PO40.2-0.5%, MgSO4·7H2O 0.01-0.06%, pH 7.2 ~ 7.4.
Fermentation condition：Same cellulase producing strain；
Above-mentioned agricultural marine alga intermediate extracts special complex micro organism fungicide compound microorganism bacterium powder application method：Take
1-5 part mix bacterium agents, the sea-tangle dry powder of 5-10 mesh is crushed to 1-5 parts glucose, 1-5 parts peptone, 1-10 parts, be well mixed
After be added in 100 parts of weight waters, 25-50 DEG C is sufficiently stirred and is incubated 0.5-8 hours, produces compound probiotic and extracts agricultural sea
Algae intermediate.
The present invention chooses a variety of different types of bacteriums and fungi and prepares complex micro organism fungicide.By marine alga content and glue
Degree detection proves, is obviously improved using the recovery rate of alginic acid in the agricultural marine alga intermediate of complex micro organism fungicide extraction,
And the composition species and content of organic molecule are obviously improved.The principle for producing above beneficial effect is：It is involved in the present invention
Each strain can produce a variety of digestive enzymes, decompose cellulose, starch, fat, nucleic acid, pectin and protein in sea-tangle etc. into
Point；Meanwhile extraction conditions of the present invention are gentle, recovery rate is high, and active ingredient retains comprehensive.
In addition, using the complex micro organism fungicide extraction marine alga intermediate in containing it is a variety of have to crops it is disease-resistant or
The probiotic microorganisms of growth-promoting functions, disease can be effectively prevented after.At the same time, the product can effectively lift soil fertilizer
Power, there is facilitation to root growth, cauline leaf growth and Fruit.Produced using the marine alga of complex micro organism fungicide extraction
Thing is of fine qualities, Nonpoisonous, non-environmental-pollution, has good water-retaining property, output increased, and significantly reduces the pest and disease damage morbidity of plant
Rate, promote multiple kinds of crops increasing both production and income.
Brief description of the drawings
Fig. 1 extracts agricultural marine alga intermediate HPLC analysis results by embodiment 2
Fig. 2 extracts marine alga intermediate HPLC analysis results by comparative example 1
Embodiment
The present invention is further described with reference to specific embodiment, so that those skilled in the art knows more about
The present invention, but and it is not so limited the present invention.
Embodiment 1
Described complex micro organism fungicide contains following strain, in parts by weight：
Bacillus subtilis 30
Trichoderma viride 20
Bacillus amyloliquefaciens 12
Bacillus cereus 12
Bacillus licheniformis 15
Aspergillus oryzae 3
Aspergillus niger 3
Bafillus natto 5
By 5 portions of above-mentioned microbial inoculums, with 3 parts of glucose, 3 parts of peptones, 7 parts of sea-tangle dry powder being crushed to below 10 mesh, mix
It is added to after uniformly in 100 parts of weight waters, 45 DEG C are sufficiently stirred and are incubated 6 hours, produce compound probiotic and extract agricultural marine alga
Intermediate.
Its marine alga acid content of acid cure Precipitation Determination is used as 1.8%, it is 25.7% to convert into butt sea-tangle recovery rate.
Embodiment 2
Described complex micro organism fungicide contains following strain, in parts by weight：
Trichoderma harzianum 25
Trichoderma viride 20
Bacillus amyloliquefaciens 15
Penicillium decumbens 10
Bacillus licheniformis 10
Bacillus thuringiensis 5
Bacillus megaterium 3
Bacillus pumilus 2
Aspergillus oryzae 5
Bacillus laterosporus 5
By 3 portions of above-mentioned microbial inoculums, with 4 parts of glucose, 4 parts of peptones, 9 parts of sea-tangle dry powder being crushed to below 10 mesh, mix
It is added to after uniformly in 100 parts of weight waters, 50 DEG C are sufficiently stirred and are incubated 8 hours, produce compound probiotic and extract agricultural marine alga
Intermediate.
Its marine alga acid content of acid cure Precipitation Determination is used as 2.5%, it is 27.8% to convert into butt sea-tangle recovery rate.
Embodiment 3
Described complex micro organism fungicide contains following strain, in parts by weight：
Bacillus subtilis 20
Trichoderma reesei 10
Koning trichoderma 10
Bacillus amyloliquefaciens 15
Goose source penicillium oxalicum 10
Penicillium decumbens 5
Bacillus thuringiensis 10
Bacillus licheniformis 5
Atrophy bacillus 3
Bacillus polymyxa 2
Aspergillus oryzae 5
Bacillus laterosporus 3
Bafillus natto 2
By 5 portions of above-mentioned microbial inoculums, with 3 parts of glucose, 5 parts of peptones, 8 parts of sea-tangle dry powder being crushed to below 10 mesh, mix
It is added to after uniformly in 100 parts of weight waters, 40 DEG C are sufficiently stirred and are incubated 8 hours, produce compound probiotic and extract agricultural marine alga
Intermediate.
Its marine alga acid content of acid cure Precipitation Determination is used as 2.1%, it is 26.3% to convert into butt sea-tangle recovery rate.
Comparative example 1
Comparative example 1 is chemical reaction flows marine alga, including following steps：
Add the water of 30 times of sea-tangle weight, and add 1.5% formaldehyde to soak 3 hours.Immersion adds 15% hydrogen-oxygen after terminating
Change sodium solution and be more than 9.0 to pH, digested.After digestion, the water dilution of 2-3 times of volume is added, is then filtered with vavuum pump.Again
Watery hydrochloric acid is slowly added into feed liquid until there is flocculent deposit, then stands 10-12 hours.Most stand in liquid and slowly add backward
Enter watery hydrochloric acid, regulation pH is about 1.5-2.0, and marine alga is condensed into acid coagulum.Determining its equivalent butt sea-tangle recovery rate is
21.4%。
Choose same batch sea-tangle, shred, crush after cross 10 mesh sieves, be divided into 4 parts of sea-tangle dry powder.Apply and implement respectively
It is as follows that example 1,2,3 and comparative example 1 handle sea-tangle dry powder acquired results：
It follows that after extracting the processing of specific complex microbial bacterial agent using agricultural marine alga intermediate, its butt alginic acid
Yield is significantly higher than control group；In addition, the marine alga intermediate organic molecule species of special bacteria agent extraction gained is also significantly more than
Control group chemical method.
Claims (5)
1. a kind of preparation process of agricultural marine alga intermediate extraction specific complex microbial bacterial agent is as follows：By more than ten kind bacterial strain difference
Deep fermentation is carried out, gained zymotic fluid is prepared as bacterium powder respectively, and agricultural marine alga intermediate is mixed to obtain by certain proportion
Extract special complex micro organism fungicide.
2. method as claimed in claim 1, it is characterised in that in described agricultural marine alga intermediate specific complex microbial bacterial agent
Strain include cellulase producing strain, production amylase strain, yielding lipase bacterial strain, production nuclease bacterial strain, production pectase bacterial strain
With the mixture of bacteria produced proteinase strain；
3. method as claimed in claim 2, it is characterised in that described cellulase producing strain includes bacillus subtilis, green
One or more of mixtures in color trichoderma, Trichoderma harzianum, trichoderma reesei, koning trichoderma；Described production amylase strain bag
Include one or more of mixtures in bacillus amyloliquefaciens, goose source penicillium oxalicum, penicillium decumbens, Paecilomyces thermaphila bacterium；Institute
The yielding lipase bacterial strain stated includes one or more of in bacillus cereus, bacillus thuringiensis, bacillus subtilis
Mixture；Described production nuclease bacterial strain includes bacillus megaterium, bacillus licheniformis, bacillus pumilus, atrophy gemma
One or more of mixtures in bacillus；Described production pectase bacterial strain includes Campinas series bacillus, aspergillus oryzae, black
One or more of mixtures in aspergillus, bacillus polymyxa；Described bacteria produced proteinase strain includes bacillus subtilis, huge
One or more of mixtures in Bacterium anthracoides, bacillus laterosporus, bafillus natto；
4. method as claimed in claim 1, it is characterised in that described agricultural marine alga intermediate extracts special complex microorganism
Each producing enzyme microbial inoculum proportion is as follows in parts by weight in microbial inoculum formula：
Cellulase-producing microbial inoculum 30-50
Produce amylase microbial inoculum 20-40
Yielding lipase microbial inoculum 10-20
Produce nuclease microbial inoculum 10-20
Produce pectase microbial inoculum 5-10
Bacteria produced proteinase agent 5-10
5. method as claimed in claim 1, it is characterised in that described agricultural marine alga intermediate extracts special complex microorganism
Microbial inoculum compound microorganism bacterium powder application method is：1-5 part mix bacterium agents are taken, with 1-5 parts glucose, 1-5 parts peptone, 1-10 parts
The sea-tangle dry powder of 5-10 mesh is crushed to, is added to after well mixed in 100 parts of weight waters, 25-50 DEG C is sufficiently stirred and is incubated
0.5-8 hours, produce compound probiotic and extract agricultural marine alga intermediate.
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