CN102276326A - Sargassum organic liquid fertilizer and preparation method thereof - Google Patents

Sargassum organic liquid fertilizer and preparation method thereof Download PDF

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Publication number
CN102276326A
CN102276326A CN2011101845247A CN201110184524A CN102276326A CN 102276326 A CN102276326 A CN 102276326A CN 2011101845247 A CN2011101845247 A CN 2011101845247A CN 201110184524 A CN201110184524 A CN 201110184524A CN 102276326 A CN102276326 A CN 102276326A
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preparation
marine alga
fermentation
organic liquid
liquid fertilizer
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CN102276326B (en
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张树清
刘培京
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Institute of Agricultural Resources and Regional Planning of CAAS
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Institute of Agricultural Resources and Regional Planning of CAAS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention discloses a sargassum organic liquid fertilizer and a preparation method thereof. The preparation method specially comprises the following steps: (1) ingredient treatment: adding 0.1-0.6 mass part of complex enzyme and 0.1-0.4 mass part of biological leaven to 100 mass parts of sargassum smashing liquid containing 70%-90% of water and then evenly mixing; (2) aerobic fermentation: putting the ingredient obtained in the step (1) in a fermentation tank, carrying out aerobic fermentation for 5-8 days at the temperature of 40-60 DGE C at the pH of 5-7 so as to obtain a fermentation broth; (3) filtration: fertilizing the fermentation broth obtained in the step (2) so as to obtain filtrate; and (4) complex formulation: blending the nutrition in the filtrate obtained in the step (3) so that the contents of nitrogen, phosphorus and potassium in the filtrate meet the following formula: N+P2O5+K2O>=100g/L. The preparation method disclosed by the invention has the advantages of quick fermentation, short production period and high fermentation degree, and is suitable for a large-scale production method for preparing the sargassum organic liquid fertilizer by virtue of a biological fermentation method.

Description

A kind of marine alga organic liquid fertilizer and preparation method thereof
Technical field
The invention belongs to the manufacturing field of organic fertilizer and organic fertilizer, be specifically related to a kind of marine alga organic liquid fertilizer and preparation method thereof.
Background technology
In marine algas such as sea-tangle, bulk kelp, bladder wrack, extra large capsule algae, kelp, be rich in the useful various inorganicss of crop growth, organism and many biologically active substances, be extraordinary agricultural fertilizer.The be applied to fertilizer of marine alga on agricultural is had a long history, many in the world maritime nations just gather marine alga a long time ago and manufacture compost, are about to pile up in heaps or be embedded in the heatable adobe sleeping platform because of stormy waves is flushed to brown alga on the seashore, make its spontaneous fermentation, make seaweed fertilizer, its agricultural produce effect is obvious.
Under the modern industrialization condition, the production method of seaweed fertilizer generally can be divided into: chemical hydrolysis (being the potassium hydroxide hydrolysis method), physics extraction method, biological fermentation process (being the enzyme liberating method).The production method of seaweed fertilizer is very big to the influence of its active substance and nutrient composition content, has both made nutrient composition content identical, and active different its fertility differences are also very big.Domestic and international most producers mainly adopt chemical hydrolysis production seaweed fertilizer at present, adopt the shortcoming of chemical extraction method maximum to be that highly basic high temperature can destroy the activity of marine alga endogenous substance; The physics extraction method is to adopt high pressure low temperature refrigerative technology to reach the purpose of alginic cell wall fragmentation, this extracting method gained seaweed fertilizer is better than the chemical extraction method, but the high pressure low temperature environment still can destroy biologically active substance in the marine alga and nutritive substance, the fertilizer efficiency of its product is still not fully up to expectations, and physical purification technical matters complexity, cost are too high.The biological fermentation ratio juris is that to utilize microorganism be the plurality of enzymes that produces in the metabolic process of nutrient with marine alga etc., the macromolecular substance that constitutes marine alga is degraded into small molecules, water miscible material, because fermenting process does not have the highly basic and the high temperature of chemical method, the high pressure and the low temperature that also do not have the physics method, therefore intactly kept biologically active substance and the nutritive substance in the marine alga to greatest extent, helped being utilized by plant absorbing.
To sum up, the best way of production seaweed fertilizer is to adopt biological fermentation process, secondly is the physics method, and the poorest is the chemical extraction method of carrying out with strong acid or highly basic.But biological fermentation process production seaweed fertilizer also has many shortcomings: such as the fermentation difficulty of macromolecular substance such as the seaweed fiber of difficult degradation in the marine alga, alginate calcium, fucoidin, protein, pectin, general fermentation about 14 days, the time of fermenting is long, causes the production cycle long; Fermentation degree is not high, and the marine alga utilization ratio is not high, causes problems such as the environmental pollution and the wasting of resources.And the technology of biological fermentation process production seaweed fertilizer, because its technology and application mechanism are lacked deep systematic study, only limit to both at home and abroad the laboratory study stage of fumbling at present, the preparation technology of mature and feasible is not applied to large-scale production.
Present various seaweed fertilizer are because the limitation of production technique separately, and the available nutrient content of seaweed fertilizer is not high, and wherein active substance lacks, and causes fertility not high, and effect is bad.
Summary of the invention
The objective of the invention is defective, provide a kind of fermentation fast, with short production cycle at above-mentioned prior art, the attenuation degree height, and the biological fermentation process of suitable large-scale production prepares the method for marine alga organic liquid fertilizer.
Another object of the present invention provides a kind of marine alga organic liquid fertilizer that adopts method for preparing.
For achieving the above object, technical scheme of the present invention is:
A kind of preparation method of marine alga organic liquid fertilizer, described preparation method are biological fermentative preparation method, specifically may further comprise the steps:
(1) batching is handled: the water content to 100 mass parts is in the marine alga pulverizing liquid of 70%-90%, adds the prozyme of 0.1-0.6 mass parts and the bio-fermentation agent of 0.1-0.4 mass parts, mixing;
(2) aerobic fermentation: step (1) gained batching is packed in the fermentor tank, is 40-60 ℃ in temperature, and pH is 5-7, aerobic fermentation 5-8 days, fermented liquid;
(3) filter:, get filtrate step (2) gained filtering fermentation liquor;
(4) composite: as nitrogen, phosphorus, potassium nutrition in step (3) the gained filtrate to be allocated, made the content of its nitrogen, phosphorus, potassium reach N+P 2O 5+ K 2O 〉=100g/L.
The water-insoluble of described filtrate is below 3%.
Described marine alga is pulverized liquid and is selected from sea-tangle and pulverizes liquid, bulk kelp and pulverize liquid and sargassun and pulverize at least a in the liquid.
Described prozyme is selected from two or more in cellulase, polygalacturonase and the proteolytic enzyme.
Described bio-fermentation agent is the fungal organism starter or/and the bacterium living beings starter, and described fungal organism starter is selected from least a in yeast, aspergillus niger and the aspergillus oryzae, and described bacterium living beings starter is that subtilis is or/and Bacillus licheniformis.
Described step (3) filtering fermentation liquor uses 80-150 purpose filter screen to filter.
During described step (4) is composite, use the content of urea allotment N, use the content of potassium primary phosphate allotment P and K.
A kind of marine alga organic liquid fertilizer that is prepared from according to above-mentioned preparation method.
The invention has the beneficial effects as follows:
Biological process disclosed by the invention prepares the method for marine alga organic liquid fertilizer, can be according to the problem of macromolecular substance such as the seaweed fiber of difficult degradation in the marine alga, alginate calcium, fucoidin, protein, pectin fermentation difficulty, the bio-fermentation agent of the prozyme and 1 ‰-4 ‰ of adding 1 ‰-6 ‰ in batching, accelerate the fermentation of marine alga, can shorten fermentation time 4-7 days than the common fermentation method, can obviously reduce the production cycle, enhance productivity; Simultaneously, bio-fermentation agent is thoroughly degraded the material of above-mentioned fermentation difficulty, makes it to be decomposed into the micromolecular polysaccharide that helps plant absorbing, monose, nitrogenous compound etc., can improve resource utilization, alleviates to the pollution of environment with to problems such as the wastings of resources.
The marine alga organic liquid fertilizer of the inventive method preparation, the micromolecular polysaccharide that macromolecular substance such as seaweed fiber, alginate calcium, fucoidin, protein, pectin is broken down into help plant absorbing, monose, nitrogenous compound etc., main active substances such as the cytokinin that simultaneously can also keep in the marine alga to be contained, plant hormone, Plant hormones regulators,gibberellins, dormin, ethene, trimethyl-glycine, polyamines, reaching the stimulation crop growth, improve crop disease-resistant, drought resisting, anti-adversity ability such as cold-resistant; Contain higher organic matter in the marine alga organic liquid fertilizer, the structure of can improving the soil.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but not as a limitation of the invention.
The preparation method of marine alga organic liquid fertilizer of the present invention specifically may further comprise the steps:
(1) batching is handled: the water content to 100 mass parts is in the marine alga pulverizing liquid of 70%-90%, adds the prozyme of 0.1-0.6 mass parts and the bio-fermentation agent of 0.1-0.4 mass parts, mixing;
(2) aerobic fermentation: step (1) gained batching is packed in the fermentor tank, is 40-60 ℃ in temperature, and pH is 5-7, aerobic fermentation 5-8 days, fermented liquid;
(3) filter:, get filtrate step (2) gained filtering fermentation liquor;
(4) composite: as nitrogen, phosphorus, potassium nutrition in step (3) the gained filtrate to be allocated, made the content of its nitrogen, phosphorus, potassium reach N+P 2O 5+ K 2O 〉=100g/L.
The water-insoluble of described filtrate is below 3%.
Described marine alga is pulverized liquid and is selected from sea-tangle and pulverizes liquid, bulk kelp and pulverize liquid and sargassun and pulverize at least a in the liquid.
Described prozyme is selected from two or more in cellulase, polygalacturonase and the proteolytic enzyme.
Described bio-fermentation agent is the fungal organism starter or/and the bacterium living beings starter, and described fungal organism starter is selected from least a in yeast, aspergillus niger and the aspergillus oryzae, and described bacterium living beings starter is that subtilis is or/and Bacillus licheniformis.
Described step (3) filtering fermentation liquor uses 80-150 purpose filter screen to filter.
During described step (4) is composite, use the content of urea allotment N, use the content of potassium primary phosphate allotment P and K.
A kind of marine alga organic liquid fertilizer that is prepared from according to above-mentioned preparation method.
According to above-mentioned preparation method, according to every index of following embodiment 1-6, the marine alga organic liquid fertilizer of preparation embodiment 1-6; As a comparison, embodiment 7-10 is the organic liquid fertilizer of traditional zymotic technology and preparation thereof.
Be specially:
Figure BDA0000073466120000041
Last table data as can be seen, embodiment 7,8 and 9,10 uses the traditional zymotic technologies, does not add prozyme and bio-fermentation agent.Embodiment 7,8 compares with embodiment 4,1, and fermentation time is identical, but the Lalgine of its gained fertilizer and organic content difference are very big, illustrates among the embodiment 7,8 that most material does not have and can decompose in the marine alga; Embodiment 9,10 compares with embodiment 4,1, and fermentation time extends to 15 days, and the Lalgine and the organic content of its gained fertilizer increase, but compares with embodiment 4,1, still has many materials not have and can decompose among the embodiment 9,10 in the marine alga.Need to prove N+P in each embodiment fertilizer 2O 5+ K 2The content of O, composite certain content, the therefore N+P of several embodiment of reaching in step 4 later 2O 5+ K 2O content is more or less the same.
Select embodiment 4,1 for use; 7,8 and 9,10 preparation gained fertilizer carry out the wheat pot experiment of fertilizer result of use, its result shows: under the rate of fertilizer situation identical with application process, the result of use of embodiment 4,1 fertilizer is best, and the biological yield of wheat uses the processing of embodiment 7,8 and 9,10 to improve 12% and 9% respectively; Grain yield improves 13% and 9% respectively.Test gathering in the crops the potted plant soil in back, the result shows, uses the potted plant soil of embodiment 4,1 fertilizer, and the content of its soil organism uses the potted plant soil of embodiment 7,8 and 9,10 to improve 24%, 19% and 18%, 15% respectively.And, the potted plant soil of use embodiment 4,1 fertilizer, soil indexs such as its crumb structure, unit weight also are better than using the potted plant soil of embodiment 7,8 and 9,10.
Need to prove that the per-cent that the present invention mentions is mass percent.
Above-described embodiment, the present invention embodiment several more preferably just, the common variation that those skilled in the art carries out in the technical solution of the present invention scope and replacing all should be included in protection scope of the present invention.

Claims (8)

1. the preparation method of a marine alga organic liquid fertilizer, it is characterized in that: described preparation method is biological fermentative preparation method, specifically may further comprise the steps:
(1) batching is handled: the water content to 100 mass parts is in the marine alga pulverizing liquid of 70%-90%, adds the prozyme of 0.1-0.6 mass parts and the bio-fermentation agent of 0.1-0.4 mass parts, mixing;
(2) aerobic fermentation: step (1) gained batching is packed in the fermentor tank, is 40-60 ℃ in temperature, and pH is 5-7, aerobic fermentation 5-8 days, fermented liquid;
(3) filter:, get filtrate step (2) gained filtering fermentation liquor;
(4) composite: as nitrogen, phosphorus, potassium nutrition in step (3) the gained filtrate to be allocated, made the content of its nitrogen, phosphorus, potassium reach N+P 2O 5+ K 2O 〉=100g/L.
2. the preparation method of a kind of marine alga organic liquid fertilizer according to claim 1 is characterized in that: the water-insoluble of described filtrate is below 3%.
3. the preparation method of a kind of marine alga organic liquid fertilizer according to claim 1 is characterized in that: described marine alga is pulverized liquid and is selected from sea-tangle and pulverizes liquid, bulk kelp and pulverize liquid and sargassun and pulverize at least a in the liquid.
4. the preparation method of a kind of marine alga organic liquid fertilizer according to claim 1 is characterized in that: described prozyme is selected from two or more in cellulase, polygalacturonase and the proteolytic enzyme.
5. the preparation method of a kind of marine alga organic liquid fertilizer according to claim 1, it is characterized in that: described bio-fermentation agent is that the fungal organism starter is or/and the bacterium living beings starter, described fungal organism starter is selected from least a in yeast, aspergillus niger and the aspergillus oryzae, and described bacterium living beings starter is that subtilis is or/and Bacillus licheniformis.
6. the preparation method of a kind of marine alga organic liquid fertilizer according to claim 1 is characterized in that: described step (3) filtering fermentation liquor, use 80-150 purpose filter screen to filter.
7. the preparation method of a kind of marine alga organic liquid fertilizer according to claim 1 is characterized in that: during described step (4) is composite, use the content of urea allotment N, use the content of potassium primary phosphate allotment P and K.
8. marine alga organic liquid fertilizer that is prepared from according to each preparation method of claim 1-7.
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CN102584432A (en) * 2012-02-05 2012-07-18 青岛丰泰海洋生物科技有限公司 Total-nutrient kelp oligosaccharide biological synergistic fertilizer and preparation method thereof
CN102731176A (en) * 2012-06-28 2012-10-17 威海市世代海洋生物科技有限公司 Production method for seaweed bio-composite organic liquid fertilizer
CN103204713A (en) * 2013-01-09 2013-07-17 中国海洋大学生物工程开发有限公司 Method for promoting high efficiency decomposition of enteromorpha powder by using enzymatically hydrolyzed sea-tangle residues and application of product
ES2504817A1 (en) * 2013-04-05 2014-10-08 Mariano DÍEZ RUIZ Procedure for obtaining a fertilizer (Machine-translation by Google Translate, not legally binding)
CN104177168A (en) * 2014-07-24 2014-12-03 四川光大制药有限公司 Organic fertilizer prepared from antiviral granule medicine dregs and preparation method thereof
CN104230402A (en) * 2014-08-26 2014-12-24 威海市世代海洋生物科技有限公司 Storage method of fresh kelps
CN104355749A (en) * 2014-11-11 2015-02-18 烟台嘉特生物技术有限公司 Production method of microbial fermented seaweed fertilizer
CN104774094A (en) * 2015-04-01 2015-07-15 大连理工大学 Preparation method of compound microbial seaweed fertilizer
CN104860731A (en) * 2015-04-03 2015-08-26 苏州仁成生物科技有限公司 Preparation method for bio-organic fertilizer capable of improving taste quality of cherry tomato
CN105110847A (en) * 2015-08-04 2015-12-02 鲍克胜 Preparation process for composite fertilizer
CN105622293A (en) * 2016-02-01 2016-06-01 青岛海大生物集团有限公司 Method for preparing salt-resistant alkali-resistant special algal biological water-soluble fertilizer by enzymatic hydrolysis fermentation
CN105906389A (en) * 2016-06-06 2016-08-31 战怡静 Preparation method and product of efficient yield-increasing seaweed fertilizer
CN106278493A (en) * 2016-07-25 2017-01-04 五洲丰农业科技有限公司 The classification enzymatic isolation method preparation method containing oligosaccharide seaweed organic fertilizer
CN106478304A (en) * 2016-11-10 2017-03-08 广西丞朔生物科技有限公司 Composite liquefied fertilizer and preparation method thereof
CN106518530A (en) * 2016-11-10 2017-03-22 广西丞朔生物科技有限公司 Compound organic fluid fertilizer and preparation method thereof
CN107619799A (en) * 2017-04-13 2018-01-23 济南航晨生物科技有限公司 A kind of agricultural marine alga intermediate extraction specific complex microbial bacterial agent and preparation method thereof
CN107628888A (en) * 2017-11-13 2018-01-26 华南农业大学 A kind of seaweed liquid fertilizer with drought resisting function and preparation method and application
CN107827544A (en) * 2017-10-26 2018-03-23 安徽华联肥业有限公司 A kind of production method of the comprehensive brown alga fermentation liquid-based bio-organic liquid fertilizer material of nutrition
CN108203729A (en) * 2017-12-28 2018-06-26 广州市科能化妆品科研有限公司 A kind of preparation method of bulk kelp anti-oxidation peptide
CN109090938A (en) * 2018-08-28 2018-12-28 北京金天龙创新科技有限公司 A kind of linseed root leaf tea, flax pillow fillers and preparation method thereof
CN109704858A (en) * 2019-01-21 2019-05-03 李永兴 A kind of method of temperate condition production alga fertilizer
CN110338374A (en) * 2019-07-30 2019-10-18 威海长青海洋科技股份有限公司 A method of brown alga extracting solution is prepared using the enzyme-linked fermentation method of microorganism
CN110590446A (en) * 2019-09-25 2019-12-20 青岛佰蓝海藻有限公司 Method for preparing liquid seaweed fertilizer by using kelp as raw material
CN110759786A (en) * 2019-12-16 2020-02-07 张林平 Seaweed water-soluble fertilizer containing fruit and vegetable juice and preparation method thereof
CN110922247A (en) * 2019-12-02 2020-03-27 烟台万丰生物科技有限公司 Organic water-soluble fertilizer and processing technology thereof
CN111165517A (en) * 2019-12-29 2020-05-19 山东劲脉植物细胞信息技术有限公司 Natural organic-inorganic anti-freezing preparation and production method thereof
CN114380642A (en) * 2022-01-29 2022-04-22 哈尔滨皮加诺生物科技有限公司 Microbial agent composition and application thereof in crop cultivation
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CN104774094A (en) * 2015-04-01 2015-07-15 大连理工大学 Preparation method of compound microbial seaweed fertilizer
CN104860731A (en) * 2015-04-03 2015-08-26 苏州仁成生物科技有限公司 Preparation method for bio-organic fertilizer capable of improving taste quality of cherry tomato
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CN105622293A (en) * 2016-02-01 2016-06-01 青岛海大生物集团有限公司 Method for preparing salt-resistant alkali-resistant special algal biological water-soluble fertilizer by enzymatic hydrolysis fermentation
CN105906389A (en) * 2016-06-06 2016-08-31 战怡静 Preparation method and product of efficient yield-increasing seaweed fertilizer
CN106278493B (en) * 2016-07-25 2018-03-09 五洲丰农业科技有限公司 It is classified the method that enzymatic isolation method prepares the seaweed organic fertilizer containing oligosaccharides
CN106278493A (en) * 2016-07-25 2017-01-04 五洲丰农业科技有限公司 The classification enzymatic isolation method preparation method containing oligosaccharide seaweed organic fertilizer
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CN107628888A (en) * 2017-11-13 2018-01-26 华南农业大学 A kind of seaweed liquid fertilizer with drought resisting function and preparation method and application
CN108203729A (en) * 2017-12-28 2018-06-26 广州市科能化妆品科研有限公司 A kind of preparation method of bulk kelp anti-oxidation peptide
CN109090938A (en) * 2018-08-28 2018-12-28 北京金天龙创新科技有限公司 A kind of linseed root leaf tea, flax pillow fillers and preparation method thereof
CN109704858A (en) * 2019-01-21 2019-05-03 李永兴 A kind of method of temperate condition production alga fertilizer
CN110338374A (en) * 2019-07-30 2019-10-18 威海长青海洋科技股份有限公司 A method of brown alga extracting solution is prepared using the enzyme-linked fermentation method of microorganism
CN110590446A (en) * 2019-09-25 2019-12-20 青岛佰蓝海藻有限公司 Method for preparing liquid seaweed fertilizer by using kelp as raw material
CN110922247A (en) * 2019-12-02 2020-03-27 烟台万丰生物科技有限公司 Organic water-soluble fertilizer and processing technology thereof
CN110759786A (en) * 2019-12-16 2020-02-07 张林平 Seaweed water-soluble fertilizer containing fruit and vegetable juice and preparation method thereof
CN111165517A (en) * 2019-12-29 2020-05-19 山东劲脉植物细胞信息技术有限公司 Natural organic-inorganic anti-freezing preparation and production method thereof
CN114380642A (en) * 2022-01-29 2022-04-22 哈尔滨皮加诺生物科技有限公司 Microbial agent composition and application thereof in crop cultivation
CN114380642B (en) * 2022-01-29 2022-09-20 哈尔滨皮加诺生物科技有限公司 Microbial agent composition and application thereof in crop cultivation
CN114538972A (en) * 2022-04-13 2022-05-27 广州世睿生物科技有限公司 Peptide-containing seaweed active substance and method for extracting seaweed active substance

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