CN106914128A - A kind of biological deodorant and preparation method thereof - Google Patents

A kind of biological deodorant and preparation method thereof Download PDF

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Publication number
CN106914128A
CN106914128A CN201710295446.5A CN201710295446A CN106914128A CN 106914128 A CN106914128 A CN 106914128A CN 201710295446 A CN201710295446 A CN 201710295446A CN 106914128 A CN106914128 A CN 106914128A
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bacillus pumilus
deodorant
biological deodorant
inoculated
seed liquor
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Inventor
任瑞霞
李楠
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Sino Forest Landscape (beijing) Eco Polytron Technologies Inc
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Sino Forest Landscape (beijing) Eco Polytron Technologies Inc
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/74General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84Biological processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/48Sulfur compounds
    • B01D53/52Hydrogen sulfide
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/54Nitrogen compounds
    • B01D53/58Ammonia
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2251/00Reactants
    • B01D2251/95Specific microorganisms
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2258/00Sources of waste gases
    • B01D2258/02Other waste gases
    • B01D2258/0266Other waste gases from animal farms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The invention discloses a kind of biological deodorant and preparation method thereof.A kind of biological deodorant provided by the present invention, the component comprising following mass percent:Bacillus pumilus zymotic fluid 10~80%, yucca extract 1~3%, ginkgo biloba p.e 1~3%, green-tea extract 1~2%, citric acid 1~2%, malic acid 1~2%, lactic acid 3~6%, essence 0.1~1%, balance of distilled water.Provided by the present invention is that one kind can suppress harmful bacterial growth, improves rubbish ecological environment, eliminates the biological deodorant of foul gas.

Description

A kind of biological deodorant and preparation method thereof
Technical field
The present invention relates to biological deodorizing technical field, and in particular to a kind of biological deodorant and preparation method thereof.
Background technology
With the development of China's animal husbandry, the scale of cultivation and intensive degree also more and more higher, environmental problem also by Gradually it is exposed.In especially existing aquaculture, the handling problems of foul smell are not focused on, bad shadow is caused to its surrounding enviroment Ring.The foul smell of aquaculture discharge is mainly hydrogen sulfide, ammonia, volatile fatty acid, trimethylamine, methane, scatol, thio-alcohol etc. Mixture.On the one hand these materials pollute to air, on the other hand produce baneful influence to nearby residents, or even can also Jeopardize the health of people.Domestic and international existing plant's waste gas disposal technology generally requires to increase equipment investment, and cost of investment is high, It is difficult to promote.Therefore, how both economical rationality, can effectively control the stench of plant again, can be held as current guarantee animal husbandry Supervention opens up problem in the urgent need to address.
Deodorant in the market, four classes can be divided into by deodorant mechanism:(1) sense organ is deodorant;(2) physical deodorization;(3) change Learn deodorant;(4) biological deodorizing.With microorganism come it is deodorant be a relatively new method, with significant advantage:Safety collar Protect, with low cost, treatment effeciency is high, it is to avoid secondary pollution etc..At this stage, microbial deodorant field has made some progress, together When also have deficiency.The patent application of such as Application No. 201510973241.9 discloses a Plant Extracts with microorganism Compound deodorizer, but strain and plant extracts described in patent is excessively complicated, and cost is too high in practical application, this use Mixed Microbes, tunning stability is poor, and batch production is not guaranteed;Furthermore first plant extracts and bacterium are mixed in this patent Ferment again afterwards, such bacterium may greatly reduce plant and carry by the deodorant ingredient degradation of plant extracts into other compositions Take the effect of thing.
Using being a kind of environment-friendly composite biological deodorant, all the components are environment-friendly composition to the present invention, and comprehensively Sense organ is deodorant, the advantage of chemical deodorizing and biological deodorizing, can quickly, thoroughly eradicate foul smell.Existing biological deodorant effect It is uneven, it is relatively costly, so, regarding to the issue above, being badly in need of one kind can improve rubbish life to suppressing harmful bacterial growth State environment, eliminates the microbial bacterial agent of the generation of foul gas.
The content of the invention
It is not enough in order to make up the above, the invention discloses a kind of biological deodorant deodorant for aquaculture and its preparation side Method, for it is quick, persistently solve the foul smell problem of open plant, environment-friendly, non-secondary pollution.
To achieve these goals, the present invention is achieved through the following technical solutions:
A kind of biological deodorant, the component comprising following mass percent:
Bacillus pumilus zymotic fluid 10~80%, yucca extract 1~3%, ginkgo biloba p.e 1~3%, green tea are carried Take thing 1~2%, citric acid 1~2%, malic acid 1~2%, lactic acid 3~6%, essence 0.1~1%, balance of distilled water.
The bacillus pumilus zymotic fluid is prepared by a method comprising the following steps and obtains:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is inoculated into seed liquor training Inoculated and cultured is carried out in foster base, bacillus pumilus seed liquor is obtained;
(2) bacillus pumilus fermentation:The bacillus pumilus seed liquor that the step (1) is obtained is inoculated into fermentation training Fermented and cultured is carried out in foster base, that is, obtains bacillus pumilus zymotic fluid;
The fermentation medium is by the material composition including following concentration:Sucrose 5.0-20.0g/L, sodium nitrate 1.0-10g/ L, ammonium chloride 1.0-10g/L, dipotassium hydrogen phosphate 2.0-20g/L, potassium dihydrogen phosphate 1.0-5.0g/L, green vitriol 0.1- 1.0g/L, magnesium sulfate 0.2-2.0g/L, yeast extract 0.2-1.0g/L.
The seed liquid culture medium is by the material composition including following mass concentration:Peptone 10.0g/L, beef extract 3.0g/L, sodium chloride 5.0g/L.
The inoculated and cultured condition is:Temperature be 37 ± 1 DEG C, pH value be 7.2 ± 0.2 under conditions of incubation time be 20 ~24h.
The condition of the fermented and cultured is:In 0.5~1m of throughput3/ h, cultivates under conditions of 35~37 DEG C of cultivation temperature 18~24h.
The preparation method of the biological deodorant, comprises the following steps:Take the component of following mass percent concentration:It is short and small Fermentation of bacillus liquid 10~80%, yucca extract 1~3%, ginkgo biloba p.e 1~3%, green-tea extract 1~2%, Citric acid 1~2%, malic acid 1~2%, lactic acid 3~6%, essence 0.1~1%, balance of distilled water, mix, and obtain final product described Biological deodorant.
The bacillus pumilus zymotic fluid is prepared by a method comprising the following steps and obtains:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is inoculated into seed liquor training Inoculated and cultured is carried out in foster base, bacillus pumilus seed liquor is obtained;
(2) bacillus pumilus fermentation:The bacillus pumilus seed liquor that the step (1) is obtained is inoculated into fermentation training Fermented and cultured is carried out in foster base, that is, obtains bacillus pumilus zymotic fluid;
The fermentation medium is by the material composition including following concentration:Sucrose 5.0-20.0g/L, sodium nitrate 1.0-10g/ L, ammonium chloride 1.0-10g/L, dipotassium hydrogen phosphate 2.0-20g/L, potassium dihydrogen phosphate 1.0-5.0g/L, green vitriol 0.1- 1.0g/L, magnesium sulfate 0.2-2.0g/L, yeast extract 0.2-1.0g/L.
Application of the described biological deodorant in aquaculture is deodorant falls within protection scope of the present invention.
The application is that the deodorant is carried out into volume ratio with water for 1:5~1:30 dilution, sprays at least 7 days.
One of feature of the invention is the formula of fermentation medium, and the formula has following features:
(1) nutrient media components typically all contains carbon source, nitrogen source and other trace elements, bacteria culture media carbon:Nitrogen ratio is 10: 1 or so, if certain culture medium is excessive, can cause to waste and environmental pollution in application process, because culture medium is also in itself Organic matter, thus we choose be nutrient media components the most basic.
(2) in environment, the abundant environment of the especially excrement inherently organic contamination in plant's environment, and nitrogen source, The main component of foul smell is ammonia, and nitrogen source includes nitrate nitrogen and ammoniacal nitrogen, and they exist in natural environment and turn and ammonia between Change is acted on.If the nitrogen source of culture medium is excessive, the nitrogen source in environment can be increased on the contrary, so as to counteract part deodorizing effect, no Can be the maximum performance of our deodorizing effect, therefore we also examine the nitrogen content in aquaculture environment when nitrogen source is selected Worry is entered, and the nitrogen content in culture medium is adjusted, and having reached can meet growth, result that also will not be superfluous.
(3) component of culture medium determines the color and luster after medium sterilization, fermentation, and we, as carbon source, are added from sucrose Micro yeast extract, the culture medium after sterilizing is presented milky, and the clean sense in place is not interfered with actual applications.
Except the formula of fermentation medium has superiority, the strain that the present invention is used also has an enormous advantage.This hair It is bright only with a kind of bacterium, i.e. bacillus pumilus, this strain is used field of deodorization is fresh, and the advantage using a strain exists In:Single bacterium prepares tunning stabilization, the product quality stabilization that batch production is obtained, and single bacterium is prepared without cultivating simultaneously Several bacterium, while also just eliminating the step of by a certain percentage mixing different bacterium, greatly reduce cost, while also simplifying Preparation process.
The present invention adds a certain amount of citric acid, malic acid and lactic acid in the formula of deodorant, these three organic acids To person poultry harmless, their molecular weight are small, and the speed that foul smell molecule reacts.The sour content added in the present invention is and supports The ammonia content grown in an environment is closely related, and three kinds of additions of acid of present invention selection had both met deodorant demand, and right Strain is not injured, and will not reduce the activity of bacterium.Meanwhile, the environment according to different breeding can slightly adjust the consumption of acid.
Specific embodiment
There is chemical reagent in specific embodiment:Sodium chloride, sucrose, sodium nitrate, ammonium chloride, dipotassium hydrogen phosphate, di(2-ethylhexyl)phosphate Hydrogen potassium, green vitriol, magnesium sulfate, citric acid, malic acid, lactic acid, purchased from Chemical Reagent Co., Ltd., Sinopharm Group.
There is biological reagent in specific embodiment:Peptone, beef extract, yeast extract is tried purchased from Chinese medicines group chemistry Agent Co., Ltd.
Yucca extract, ginkgo biloba p.e, green-tea extract, purchased from Henan Lv Bang bio tech ltd
Embodiment 1:Biological deodorant and preparation method thereof
The preparation method of biological deodorant:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is (micro- purchased from Chinese industrial Biological inoculum preservation administrative center) it is inoculated into seed liquid culture medium and is cultivated, obtain bacillus pumilus seed liquor;
Seed liquid culture medium is by the material composition including following mass concentration:Peptone 10.0g/L, beef extract 3.0g/ L, sodium chloride 5.0g/L.Modulation seed liquor medium pH is 7.2, and sterilising temp is 121 DEG C, and sterilization time is 20min.Access bacterium After kind, cultivation temperature is 37 DEG C, and incubation time is 24h, standby;
(2) bacillus pumilus fermentation:Bacillus pumilus seed liquor in step (1) is inoculated into fermentation medium Fermented and cultured is carried out, that is, obtains bacillus pumilus zymotic fluid.It is the above-mentioned hair of cumulative volume 60% to load loading amount in fermentation tank Ferment culture medium, after sterilizing, when culture medium temperature is reduced to 37 DEG C, inoculation accounts for the bacillus pumilus seed liquor of culture volume 5%, Fermentation condition is:Rotating speed 150rpm, throughput 0.7m3/ h, tank pressure 0.02Mpa, 37 DEG C of cultivation temperature, incubation time is 18h.
Fermentation medium is by the material composition including following concentration:Sucrose 10g/L, sodium nitrate 6g/L, ammonium chloride 5g/L, phosphorus Sour hydrogen dipotassium 10g/L, potassium dihydrogen phosphate 3g/L, green vitriol 0.6g/L, magnesium sulfate 1.0g/L, yeast extract 0.7g/ L;
Fermentation medium sterilising temp is 121 DEG C, and sterilization time is 20min.
(3) biological deodorant is prepared:The component of following mass percent concentration is taken respectively:Bacillus pumilus zymotic fluid 50%th, yucca extract 2%, ginkgo biloba p.e 2%, green-tea extract 1.5%, citric acid 1.5%, malic acid 1.5%, breast Acid 5%, essence 0.5%, balance of distilled water, are mixed evenly to prepare biological deodorant.
Embodiment 2:Biological deodorant and preparation method thereof
The preparation method of biological deodorant:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is inoculated into seed liquor training Cultivated in foster base, obtained bacillus pumilus seed liquor;
Seed liquid culture medium is by the material composition including following mass concentration:Peptone 10.0g/L, beef extract 3.0g/ L, sodium chloride 5.0g/L.Modulation seed liquor medium pH is 7.0, and sterilising temp is 121 DEG C, and sterilization time is 20min.Access bacterium After kind, cultivation temperature is 36 DEG C, and incubation time is 20h, standby;
(2) bacillus pumilus fermentation:Bacillus pumilus seed liquor in step (1) is inoculated into fermentation medium Fermented and cultured is carried out, that is, obtains bacillus pumilus zymotic fluid.It is the above-mentioned hair of cumulative volume 50% to load loading amount in fermentation tank Ferment culture medium, after sterilizing, when culture medium temperature is reduced to 37 DEG C, inoculation accounts for the bacillus pumilus seed of culture volume 10% Liquid, fermentation condition is:Rotating speed 150rpm, throughput 0.5m3/ h, tank pressure 0.01Mpa, 35 DEG C of cultivation temperature, incubation time is 18h。
Fermentation medium is by the material composition including following concentration:Sucrose 5.0g/L, sodium nitrate 1.0g/L, ammonium chloride 1.0g/L, dipotassium hydrogen phosphate 2.0g/L, potassium dihydrogen phosphate 1.0g/L, green vitriol 0.1g/L, magnesium sulfate 0.2g/L, ferment Female medicinal extract 0.2g/L;
Fermentation medium sterilising temp is 121 DEG C, and sterilization time is 20min;
(3) biological deodorant is prepared:The component of following mass percent concentration is taken respectively:Bacillus pumilus zymotic fluid 10%th, yucca extract 1%, ginkgo biloba p.e 1%, green-tea extract 1%, citric acid 1%, malic acid 1%, lactic acid 3%, Essence 0.1%, balance of distilled water, are mixed evenly to prepare biological deodorant.
Embodiment 3:Biological deodorant and preparation method thereof
The preparation method of biological deodorant:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is inoculated into seed liquor training Cultivated in foster base, obtained bacillus pumilus seed liquor;Seed liquid culture medium is by the material group including following mass concentration Into:Peptone 10.0g/L, beef extract 3.0g/L, sodium chloride 5.0g/L.Modulation seed liquor medium pH is 7.4, sterilising temp It it is 121 DEG C, sterilization time is 20min.After accessing strain, cultivation temperature is 38 DEG C, and incubation time is 24h, standby;
(2) bacillus pumilus fermentation:Bacillus pumilus seed liquor in step (1) is inoculated into fermentation medium Fermented and cultured is carried out, that is, obtains bacillus pumilus zymotic fluid.It is the above-mentioned hair of cumulative volume 70% to load loading amount in fermentation tank Ferment culture medium, after sterilizing, when culture medium temperature is reduced to 45 DEG C, inoculation accounts for the bacillus pumilus seed liquor of culture volume 1%, Fermentation condition is:Rotating speed 200rpm, throughput 1m3/ h, tank pressure 0.03Mpa, 37 DEG C of cultivation temperature, incubation time is 24h.;
Fermentation medium is by the material composition including following concentration:Sucrose 20.0g/L, sodium nitrate 10g/L, ammonium chloride 10g/ L, dipotassium hydrogen phosphate 20g/L, potassium dihydrogen phosphate 5.0g/L, green vitriol 1.0g/L, magnesium sulfate 2.0g/L, yeast extract 1.0g/L;
Fermentation medium sterilising temp is 121 DEG C, and sterilization time is 20min;
(3) biological deodorant is prepared:The component of following mass percent concentration is taken respectively:Bacillus pumilus zymotic fluid 80%th, yucca extract 3%, ginkgo biloba p.e 3%, green-tea extract 2%, citric acid 2%, malic acid 2%, lactic acid 6%, Essence 1%, balance of distilled water, are mixed evenly to prepare biological deodorant.
Embodiment 4:Plant's Deodorization Experiment 1
Experimental Area is independent pigsty.The deodorant that embodiment 1 is produced carries out 1 with water:After the dilution of 20 (volume ratios), With atomizers spray, each sprinkling is once, as shown in the table using the effect after 7 days sooner or later.
1 deodorizing effect of biological deodorant of table
Foul smell composition Concentration before implementation Concentration after implementation Clearance
2.07ppm 0.40ppm 81%
0.03ppm 0ppm 100%
Sense organ Stink is obvious Foul smell is reduced /
Mosquitos and flies situation A lot Reduce about 80% /
The biological deodorant to embodiment 2 and embodiment 3 carries out plant's Deodorization Experiment in aforementioned manners, as a result without significantly Difference.
From above-mentioned, the content that biological deodorant of the invention has good deodorizing effect, ammonia to plant is reduced Original 1/5th are arrived, not detect, to ammonia, hydrogen sulfide removal effect is significant, the sense organ of people can also be felt hydrogen sulfide content Feel obvious improvement, mosquito has also been lacked a lot.
Embodiment 5:Plant's Deodorization Experiment 2
Experimental Area is independent pigsty.The deodorant that embodiment 1 is produced carries out 1 with water:After the dilution of 30 (volume ratios), With atomizers spray, each sprinkling is once, as shown in the table using the effect after 30 days sooner or later.
2 deodorizing effects of biological deodorant of table
Foul smell composition Concentration before implementation Concentration after implementation Clearance
2.10ppm 0.30ppm 86%
0.04ppm 0ppm 100%
Sense organ Stink is obvious Foul smell is reduced /
Mosquitos and flies situation A lot Reduce about 80% /
From above-mentioned, the content that biological deodorant of the invention has good deodorizing effect, ammonia to plant is reduced Original 1/7th are arrived, not detect, to ammonia, hydrogen sulfide removal effect is significant, the sense organ of people can also be felt hydrogen sulfide content Feel obvious improvement, mosquito has also been lacked a lot.
The biological deodorant to embodiment 2 and embodiment 3 carries out plant's Deodorization Experiment in aforementioned manners, as a result without significantly Difference.
Embodiment 6:Plant's Deodorization Experiment 3
Experimental Area is independent pigsty.The deodorant that embodiment 1 is produced carries out 1 with water:After the dilution of 5 (volume ratios), With atomizers spray, each sprinkling is once, as shown in the table using the effect after 10 days sooner or later.
3 deodorizing effects of biological deodorant of table
Foul smell composition Concentration before implementation Concentration after implementation Clearance
2.16ppm 0.22ppm 90%
0.04ppm 0ppm 100%
Sense organ Stink is obvious Foul smell is reduced /
Mosquitos and flies situation A lot Reduce about 80% /
From above-mentioned, the content that biological deodorant of the invention has good deodorizing effect, ammonia to plant is reduced Original 1/10th are arrived, not detect, to ammonia, hydrogen sulfide removal effect is significant, the sense organ of people can also be felt hydrogen sulfide content Feel obvious improvement, mosquito has also been lacked a lot.
The biological deodorant to embodiment 2 and embodiment 3 carries out plant's Deodorization Experiment in aforementioned manners, as a result without significantly Difference.
The new deodorant and its preparation method that this more special environment is developed the present invention be directed to plant, The working environment of the staff of plant is not only improved, is also obviously improved the growing environment quality of domestic animal, Have a very big significance.

Claims (9)

1. a kind of biological deodorant, it is characterised in that the component comprising following mass percent:
Bacillus pumilus zymotic fluid 10~80%, yucca extract 1~3%, ginkgo biloba p.e 1~3%, green-tea extract 1~2%, citric acid 1~2%, malic acid 1~2%, lactic acid 3~6%, essence 0.1~1%, balance of distilled water.
2. biological deodorant according to claim 1, it is characterised in that:The bacillus pumilus zymotic fluid is by bag Include what the method for following steps was prepared:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is inoculated into seed liquid culture medium In carry out inoculated and cultured, obtain bacillus pumilus seed liquor;
(2) bacillus pumilus fermentation:The bacillus pumilus seed liquor that the step (1) is obtained is inoculated into fermentation medium In carry out fermented and cultured, that is, obtain bacillus pumilus zymotic fluid;
The fermentation medium is by the material composition including following concentration:Sucrose 5.0-20.0g/L, sodium nitrate 1.0-10g/L, chlorine Change ammonium 1.0-10g/L, dipotassium hydrogen phosphate 2.0-20g/L, potassium dihydrogen phosphate 1.0-5.0g/L, green vitriol 0.1- 1.0g/L, magnesium sulfate 0.2-2.0g/L, yeast extract 0.2-1.0g/L.
3. biological deodorant according to claim 2, it is characterised in that:The seed liquid culture medium is by including following quality The material composition of concentration:Peptone 10.0g/L, beef extract 3.0g/L, sodium chloride 5.0g/L.
4. biological deodorant according to claim 2, it is characterised in that:The inoculated and cultured condition is:It is 37 in temperature ± 1 DEG C, pH value be 7.2 ± 0.2 under conditions of incubation time be 20~24h.
5. biological deodorant according to claim 2, it is characterised in that:The condition of the fermented and cultured is:In throughput 0.5 ~1m318~24h is cultivated under conditions of/h, 35~37 DEG C of cultivation temperature.
6. in claim 1-5 any described biological deodorant preparation method, comprise the following steps:Take following quality percentage The component of specific concentration:Bacillus pumilus zymotic fluid 10~80%, yucca extract 1~3%, ginkgo biloba p.e 1~3%, Green-tea extract 1~2%, citric acid 1~2%, malic acid 1~2%, lactic acid 3~6%, essence 0.1~1%, balance of distillation Water, mixes, and obtains final product the biological deodorant.
7. preparation method according to claim 6, it is characterised in that:The bacillus pumilus zymotic fluid is by including What the method for following steps was prepared:
(1) preparation of seed liquor:By preserving number for the bacillus pumilus strain of CICC 21901 is inoculated into seed liquid culture medium In carry out inoculated and cultured, obtain bacillus pumilus seed liquor;
(2) bacillus pumilus fermentation:The bacillus pumilus seed liquor that the step (1) is obtained is inoculated into fermentation medium In carry out fermented and cultured, that is, obtain bacillus pumilus zymotic fluid;
The fermentation medium is by the material composition including following concentration:Sucrose 5.0-20.0g/L, sodium nitrate 1.0-10g/L, chlorine Change ammonium 1.0-10g/L, dipotassium hydrogen phosphate 2.0-20g/L, potassium dihydrogen phosphate 1.0-5.0g/L, green vitriol 0.1- 1.0g/L, magnesium sulfate 0.2-2.0g/L, yeast extract 0.2-1.0g/L.
8. application of any described biological deodorant in aquaculture is deodorant in claim 1-5.
9. the application according to claim 8, it is characterised in that:The application is that the deodorant is carried out into volume with water Than being 1:5~1:30 dilution, sprays at least 7 days.
CN201710295446.5A 2017-04-28 2017-04-28 A kind of biological deodorant and preparation method thereof Pending CN106914128A (en)

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CN107638795A (en) * 2017-10-18 2018-01-30 贵州德莱易生物科技有限公司 One kind de-tastes degerming spray
CN108097032A (en) * 2017-12-29 2018-06-01 广州广牧丰生物技术有限公司 A kind of farm's deodorant and the method that poultry production performance is improved with the deodorant
CN108246094A (en) * 2017-12-29 2018-07-06 广州广牧丰生物技术有限公司 A kind of composite highly effective liquid bio deodorant and preparation method thereof
CN109646361A (en) * 2019-02-27 2019-04-19 山东信得科技股份有限公司 A kind of pet complex biological deodorization spray agent and preparation method thereof
CN110180874A (en) * 2019-06-24 2019-08-30 湖南省土壤肥料研究所 A kind of new and effective fragrant deodorant of biology system

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