CN104307012A - Deodorant special for livestock and poultry farms and application thereof - Google Patents
Deodorant special for livestock and poultry farms and application thereof Download PDFInfo
- Publication number
- CN104307012A CN104307012A CN201410615491.0A CN201410615491A CN104307012A CN 104307012 A CN104307012 A CN 104307012A CN 201410615491 A CN201410615491 A CN 201410615491A CN 104307012 A CN104307012 A CN 104307012A
- Authority
- CN
- China
- Prior art keywords
- culture
- individual
- culture fluid
- bacterium
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a deodorant special for livestock and poultry farms and application thereof, wherein the deodorant is composed of the following strains: 106-108 lactobacillus acidophilus per milliliter, 107-109 lactobacillus reuteri per milliliter, 104-106 bacillus polymyxa per milliliter, 106-108 bacillus lentus per milliliter, 106-109 bacillus coagulans per milliliter, 104-106 enterococcus faecalis per milliliter, 107-109 enterococcus faecium per milliliter, 104-107 candida rugosa per milliliter, 104-107 candida valida per milliliter, 104-106 pseudomonas farinofermentans per milliliter, 106-108 rhodopseudomonas palustris per milliliter, 104-106 geotrichum suaveolens per milliliter, 106-108 aspergillus clavatus per milliliter, 106-108 rough spore aspergillus per milliliter and 106-109 streptomyces globisporus per milliliter. According to the invention, excrements in a poultry house and excrements for accumulative fermentation are treated through a spraying manner, so as to effectively reduce the release amount of malodorous gases, and provide a technical support for the effective treatment of malodor in the livestock and poultry farms and the environmental protection. Besides, the deodorant special for livestock and poultry farms can also be sprayed to the livestock and poultry body surfaces, to keep the livestock and poultry body surfaces clean and sanitary, and be conducive to the growth of livestock and poultry.
Description
Technical field
The present invention relates to a kind of deodorizer, specifically relate to the special deodorizer of a kind of livestock and poultry farm and application thereof, belong to environmental protection technical field.
Background technology
Along with the large-scale development of livestock and poultry breeding industry, the pollution of farm animal excrement to plant's surrounding is also on the rise, and wherein producing foul gas is one of major way.The main cause causing feces of livestock and poultry foul gas to produce is volatilization and the release of the material such as ammonia, hydrogen sulfide, methane and organic acid in feces banking process.Foul gas brings discomfort in olfactory sensation not only to plant environment resident, and has impact to the breathing of people, digestion, cardiovascular, endocrine and nervous system; Live in for a long time in odor pollution environment and also can cause the functional diseases such as anorexia, insomnia, hypomnesis; Suction stench formaldehyde and benzene have strong carcinogenesis; High concentration stench can also make contactee that pulmonary edema even death by suffocation occurs.Therefore, the reasonable process of plant's foul gas is the important step controlling plant's environmental health, improve epidemic prevention condition and minimizing environmental pollution, is also the important measures ensureing that livestock and poultry breeding industry develops in a healthy way.
At present, the method that process foul gas adopts mainly contain combustion method, oxidizing process, absorption process, absorption method, in and deodorization method etc. multiple, but all exist treatment effeciency low, produce secondary pollution, the deficiency such as high to the requirement of equipment, not easy to operate.Therefore, seeking deodorizing method that is efficient, environmental protection is the advanced subject of Present Domestic odor pollution Controlling research, and one of them is exactly microbial deodorant method.This not only can decompose feces of livestock and poultry due to microorganism to produce the foul gass such as ammonia, hydrogen sulfide and methane during the fermentation, and multiple mineral acid can be produced, formed and be unfavorable for the sour environment that putrefactive microorganisms is lived, and fundamentally degraded produces the material of foul gas.Therefore, microbial deodorant method has the incomparable superiority of traditional physical chemistry deodorizing method, progressively highlights mastery reaction at stench or toxic and harmful pollution control technology field.
Using microbe deodorizer process plant stench, can be divided into regulation and control and external regulation and control two kinds of forms in body.Regulation and control aspect is in vivo concentrated in the research of current most scholar, as using microorganism as feed additive, to increase digesting and assimilating thus reducing foul smell discharge of forage protein, but regulation and control belong to indirect deodorization in body, DeGrain, especially using microorganism as feed additive, also may have potential pathogenic, substitute and shift antibiotics resistance gene, upsetting intact animal's microbiota, producing the hidden danger such as unwanted metabolic products.The research of regulation and control aspect in vitro mainly concentrates on the detection of single deodorization bacteria selection and deodorizing effect, and the complicated component of foul gas, comprise the odorants such as ammonia, hydrogen sulfide, methane and organic acid, and the removal of these odorants needs the comprehensive function of multiple-microorganism just can reach obvious effect.
Summary of the invention
The object of the present invention is to provide the special deodorizer of a kind of livestock and poultry farm, with the Excreta of spray pattern process poultry house and heap fermentation, in addition can also be sprayed onto poultry body surface, keep poultry body surface sanitation and hygiene, be conducive to the growth of poultry.The special deodorizer of this livestock and poultry farm not only can reduce the foul gas in air, effectively can also degrade and produce the material of foul gas, the generation of odorant is weakened from root, have the incomparable advantage of traditional method and safety, to overcome, the treatment effeciency that traditional method exists is low, cost is high and there is the deficiencies such as certain potential safety hazard.
The technical solution adopted for the present invention to solve the technical problems is as follows:
The special deodorizer of a kind of livestock and poultry farm, is made up of following microorganism fungus kind: bacillus acidophilus 10
6~ 10
8individual/ml, Lactobacillus reuteri 10
7~ 10
9individual/ml, bacillus polymyxa 10
4~ 10
6individual/ml, bacillus lentus 10
6~ 10
8individual/ml, Bacillus coagulans 10
6~ 10
9individual/ml, enterococcus faecalis 10
4~ 10
6individual/ml, enterococcus faecalis 10
7~ 10
9individual/ml, fold candida bacterium 10
4~ 10
7individual/ml, Candida valida bacterium 10
4~ 10
7individual/ml, Co-158 strain 10
4~ 10
6individual/ml, Rhodopseudomonas palustris 10
6~ 10
8individual/ml, Geotrichum Suaveolens 10
4~ 10
6individual/ml, excellent inulinase 10
6~ 10
8individual/ml, rough spore inulinase 10
6~ 10
8individual/ml and styreptomyces globispotus strain 10
6~ 10
9individual/ml;
The preparation method of described bacillus acidophilus and Lactobacillus reuteri:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 8 ~ 10g, peptone 8 ~ 10g, yeast extract 4 ~ 6g, glucose 3 ~ 5g, Tween 80 0.5ml, sodium acetate 3 ~ 5g, sodium citrate 2 ~ 4g, dipotassium hydrogen phosphate 1 ~ 2g, magnesium sulfate 0.4 ~ 0.6g, manganese sulfate 0.2 ~ 0.3g, ferrous sulfate 0.1 ~ 0.2g, distilled water 1000g; All culture medium raw materials carry out sterilization treatment before use;
(2) fermentation culture: measure two parts and load in two test tubes by culture medium 5 ~ 10ml that above-mentioned raw materials is formulated, aseptically, access in two test tubes with the bacillus acidophilus on inoculating loop respectively picking culture dish and Lactobacillus reuteri, shaken cultivation 6 ~ 12h under 30 ~ 35 DEG C of conditions, add the plant extract 0.5 ~ 1g through sterilization treatment afterwards, continue cultivation 12 ~ 24h, obtain the culture of bacillus acidophilus and Lactobacillus reuteri;
The preparation method of described plant extract is: by Fructus Lycii and Fructus Mori 1:2 mixing in mass ratio, in containing the sodium chloride solution of 1 ~ 3%, soak 12 ~ 24h, soaking temperature is 35 ~ 37 DEG C, soaks and terminates rear filtration, get filtering residue; Be placed in drying baker by filtering residue and dry, make its moisture lower than 12%, bake out temperature is 55 ~ 65 DEG C, pulverizes, and crosses 60 mesh sieves, obtains the powder of Fructus Lycii and Fructus Mori; In powder, add the ethanol of 80 ~ 85%, at 60 ~ 70 DEG C of temperature, steaming and decocting 3 ~ 5h, gets filtrate, filtrate is concentrated, spraying dry, pulverizes, and crosses 60 mesh sieves, obtains plant extract;
The preparation method of described bacillus polymyxa, bacillus lentus and Bacillus coagulans:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 25 ~ 30g, peptone 5 ~ 8g, glucose 15 ~ 20g, sodium chloride 3 ~ 5g, sodium hydrogen phosphate 2 ~ 3g, magnesium chloride 0.2 ~ 0.4g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid are housed respectively by bacillus polymyxa, bacillus lentus and Bacillus coagulans on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described enterococcus faecalis and enterococcus faecalis:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 3 ~ 5g, peptone 10 ~ 15g, sodium chloride 3 ~ 5g, sucrose 8 ~ 10g, dipotassium hydrogen phosphate 1 ~ 2g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare enterococcus faecalis culture fluid and enterococcus faecalis culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that enterococcus faecalis culture fluid and enterococcus faecalis culture fluid are housed respectively with enterococcus faecalis and enterococcus faecalis on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described fold candida bacterium and Candida valida bacterium:
(1) composition of culture medium: yeast extract 2 ~ 4g, peptone 4 ~ 6g, glucose 3 ~ 6g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida bacteria culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that fold candida bacteria culture fluid and Candida valida bacteria culture fluid are housed respectively with fold candida bacterium and Candida valida bacterium on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Co-158 strain and Rhodopseudomonas palustris:
(1) composition of culture medium: ammonium chloride 2 ~ 4g, dipotassium hydrogen phosphate 0.5 ~ 1g, magnesium chloride 0.2 ~ 0.4g, sodium chloride 2 ~ 4g, yeast extract 0.5 ~ 1g, magnesium sulfate 1 ~ 2g, sodium thiosulfate 0.4 ~ 0.6g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Co-158 strain culture fluid and Rhodopseudomonas palustris culture fluid are housed respectively with Co-158 strain and Rhodopseudomonas palustris on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain:
(1) composition of culture medium: sucrose 25 ~ 30g, peptone 10 ~ 15g, magnesium sulfate 0.5 ~ 1g, potassium chloride 0.5 ~ 1g, ferrous sulfate 0.01 ~ 0.03g, dipotassium hydrogen phosphate 1 ~ 2g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Geotrichum Suaveolens culture fluid, excellent inulinase culture fluid, rough spore inulinase culture fluid and styreptomyces globispotus strain culture fluid are housed respectively with Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of the special deodorizer of a kind of livestock and poultry farm is as follows:
The liquid culture of each bacterium is added in poultry deodorizer special culture solution, make each bacterium reach above-mentioned strain concentration, mix homogeneously;
Described poultry deodorizer special culture solution is made up of following material: Carnis Bovis seu Bubali cream 10 ~ 15g, peptone 3 ~ 5g, maltose 3 ~ 5g, glucose 2 ~ 4g, sodium chloride 1 ~ 2g, potassium dihydrogen phosphate 1 ~ 2g, ferrous sulfate 2 ~ 4g, magnesium sulfate 1 ~ 2g, zinc sulfate 1 ~ 2g, distilled water 1000g.
The using method of the special deodorizer of a kind of livestock and poultry farm: every day is sooner or later each in poultry house sprays once, every day consumption 0.3 ~ 0.6kg/m
3.
Beneficial effect
The present invention is applicable to process the foul smell in livestock and poultry farm house ground Excreta, house in empty face and the excrement dirt with it of pig body; effectively can reduce the foul gas in air; and reduce the burst size of foul gas from root, for the process of livestock and poultry farm stench and environmental conservation provide technical support.The present invention, compared with traditional method, has following advantage:
(1) odor removal efficient is high: the present invention microorganism fungus kind used is all through that screening obtains, and through organic assembling, greatly can improve deodorizing effect, effectively reduce the burst size of livestock and poultry farm foul gas;
(2) safety and environmental protection: the present invention not containing chemical drugs, employing be pure microbial treatment method, can not cause secondary pollution, and each strain is all conventional strains, safety is secure;
(3) strong adaptability of strain: by the organic assembling of multiple strain and the meticulous preparation of culture fluid, ensure that deodorizer can remain efficient deodorizing effect under normality condition;
(4) play a role greatly: the excrement that the present invention both may be used for processing in poultry house is dirty, the foul gas in poultry house of can effectively degrading again in air.Meanwhile, because deodorant liquid becomes neutral, pig body is not almost stimulated, also can be sprayed onto pig surface, keep poultry body surface sanitation and hygiene, be conducive to the growth of poultry.
Specific embodiment
The present invention is further described below by way of specific embodiment.
Embodiment 1
The special deodorizer of a kind of livestock and poultry farm, is made up of following microorganism fungus kind: bacillus acidophilus 10
6individual/ml, Lactobacillus reuteri 10
7individual/ml, bacillus polymyxa 10
4individual/ml, bacillus lentus 10
6individual/ml, Bacillus coagulans 10
6individual/ml, enterococcus faecalis 10
4individual/ml, enterococcus faecalis 10
7individual/ml, fold candida bacterium 10
4individual/ml, Candida valida bacterium 10
4individual/ml, Co-158 strain 10
4individual/ml, Rhodopseudomonas palustris 10
6individual/ml, Geotrichum Suaveolens 10
4individual/ml, excellent inulinase 10
6individual/ml, rough spore inulinase 10
6individual/ml and styreptomyces globispotus strain 10
6individual/ml;
The preparation method of described bacillus acidophilus and Lactobacillus reuteri:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 8g, peptone 8g, yeast extract 4g, glucose 3g, Tween 80 0.5ml, sodium acetate 3g, sodium citrate 2g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.4g, manganese sulfate 0.2g, ferrous sulfate 0.1g, distilled water 1000g; All culture medium raw materials carry out sterilization treatment before use;
(2) fermentation culture: measure two parts and load in two test tubes by the culture medium 5ml that above-mentioned raw materials is formulated, aseptically, access in two test tubes with the bacillus acidophilus on inoculating loop respectively picking culture dish and Lactobacillus reuteri, shaken cultivation 6h under 30 DEG C of conditions, add the plant extract 0.5g through sterilization treatment afterwards, continue to cultivate 12h, obtain the culture of bacillus acidophilus and Lactobacillus reuteri;
The preparation method of described plant extract is: by Fructus Lycii and Fructus Mori 1:2 mixing in mass ratio, in containing the sodium chloride solution of 1%, soak 12h, soaking temperature is 35 DEG C, soaks and terminates rear filtration, get filtering residue; Be placed in drying baker by filtering residue and dry, make its moisture lower than 12%, bake out temperature is 55 DEG C, pulverizes, and crosses 60 mesh sieves, obtains the powder of Fructus Lycii and Fructus Mori; In powder, add the ethanol of 80%, at 60 DEG C of temperature, steaming and decocting 3h, gets filtrate, filtrate is concentrated, spraying dry, pulverizes, and crosses 60 mesh sieves, obtains plant extract;
The preparation method of described bacillus polymyxa, bacillus lentus and Bacillus coagulans:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 25g, peptone 5g, glucose 15g, sodium chloride 3g, sodium hydrogen phosphate 2g, magnesium chloride 0.2g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid are housed respectively by bacillus polymyxa, bacillus lentus and Bacillus coagulans on inoculating loop picking culture dish, carry out shaken cultivation 24h under 26 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described enterococcus faecalis and enterococcus faecalis:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 3g, sucrose 8, dipotassium hydrogen phosphate 1g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare enterococcus faecalis culture fluid and enterococcus faecalis culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that enterococcus faecalis culture fluid and enterococcus faecalis culture fluid are housed respectively with enterococcus faecalis and enterococcus faecalis on inoculating loop picking culture dish, carry out shaken cultivation 24h under 26 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described fold candida bacterium and Candida valida bacterium:
(1) composition of culture medium: yeast extract 2g, peptone 4g, glucose 3g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida bacteria culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that fold candida bacteria culture fluid and Candida valida bacteria culture fluid are housed respectively with fold candida bacterium and Candida valida bacterium on inoculating loop picking culture dish, carry out shaken cultivation 24h under 26 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Co-158 strain and Rhodopseudomonas palustris:
(1) composition of culture medium: ammonium chloride 2g, dipotassium hydrogen phosphate 0.5g, magnesium chloride 0.2g, sodium chloride 2g, yeast extract 0.5g, magnesium sulfate 1g, sodium thiosulfate 0.4g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Co-158 strain culture fluid and Rhodopseudomonas palustris culture fluid are housed respectively with Co-158 strain and Rhodopseudomonas palustris on inoculating loop picking culture dish, carry out shaken cultivation 24h under 26 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain:
(1) composition of culture medium: sucrose 25g, peptone 10g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, dipotassium hydrogen phosphate 1g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Geotrichum Suaveolens culture fluid, excellent inulinase culture fluid, rough spore inulinase culture fluid and styreptomyces globispotus strain culture fluid are housed respectively with Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain on inoculating loop picking culture dish, carry out shaken cultivation 24h under 26 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of the special deodorizer of a kind of livestock and poultry farm is as follows:
The liquid culture of each bacterium is added in poultry deodorizer special culture solution, make each bacterium reach above-mentioned strain concentration, mix homogeneously;
Described poultry deodorizer special culture solution is made up of following material: Carnis Bovis seu Bubali cream 10g, peptone 3g, maltose 3g, glucose 2g, sodium chloride 1g, potassium dihydrogen phosphate 1g, ferrous sulfate 2g, magnesium sulfate 1g, zinc sulfate 1g, distilled water 1000g.
The using method of the special deodorizer of a kind of livestock and poultry farm: every day is sooner or later each in poultry house sprays once, every day consumption 0.3kg/m
3.
Embodiment 2
The special deodorizer of a kind of livestock and poultry farm, is made up of following microorganism fungus kind: bacillus acidophilus 10
8individual/ml, Lactobacillus reuteri 10
9individual/ml, bacillus polymyxa 10
6individual/ml, bacillus lentus 10
8individual/ml, Bacillus coagulans 10
9individual/ml, enterococcus faecalis 10
6individual/ml, enterococcus faecalis 10
9individual/ml, fold candida bacterium 10
7individual/ml, Candida valida bacterium 10
7individual/ml, Co-158 strain 10
6individual/ml, Rhodopseudomonas palustris 10
8individual/ml, Geotrichum Suaveolens 10
6individual/ml, excellent inulinase 10
8individual/ml, rough spore inulinase 10
8individual/ml and styreptomyces globispotus strain 10
9individual/ml;
The preparation method of described bacillus acidophilus and Lactobacillus reuteri:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 10g, peptone 10g, yeast extract 6g, glucose 5g, Tween 80 0.5ml, sodium acetate 5g, sodium citrate 4g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.6g, manganese sulfate 0.3g, ferrous sulfate 0.2g, distilled water 1000g; All culture medium raw materials carry out sterilization treatment before use;
(2) fermentation culture: measure two parts and load in two test tubes by the culture medium 10ml that above-mentioned raw materials is formulated, aseptically, access in two test tubes with the bacillus acidophilus on inoculating loop respectively picking culture dish and Lactobacillus reuteri, shaken cultivation 12h under 35 DEG C of conditions, add the plant extract 1g through sterilization treatment afterwards, continue to cultivate 24h, obtain the culture of bacillus acidophilus and Lactobacillus reuteri;
The preparation method of described plant extract is: by Fructus Lycii and Fructus Mori 1:2 mixing in mass ratio, in containing the sodium chloride solution of 3%, soak 24h, soaking temperature is 37 DEG C, soaks and terminates rear filtration, get filtering residue; Be placed in drying baker by filtering residue and dry, make its moisture lower than 12%, bake out temperature is 65 DEG C, pulverizes, and crosses 60 mesh sieves, obtains the powder of Fructus Lycii and Fructus Mori; In powder, add the ethanol of 85%, at 70 DEG C of temperature, steaming and decocting 5h, gets filtrate, filtrate is concentrated, spraying dry, pulverizes, and crosses 60 mesh sieves, obtains plant extract;
The preparation method of described bacillus polymyxa, bacillus lentus and Bacillus coagulans:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 30g, peptone 8g, glucose 20g, sodium chloride 5g, sodium hydrogen phosphate 3g, magnesium chloride 0.4g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid are housed respectively by bacillus polymyxa, bacillus lentus and Bacillus coagulans on inoculating loop picking culture dish, carry out shaken cultivation 36h under 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described enterococcus faecalis and enterococcus faecalis:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 5g, peptone 15g, sodium chloride 5g, sucrose 10g, dipotassium hydrogen phosphate 2g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare enterococcus faecalis culture fluid and enterococcus faecalis culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that enterococcus faecalis culture fluid and enterococcus faecalis culture fluid are housed respectively with enterococcus faecalis and enterococcus faecalis on inoculating loop picking culture dish, carry out shaken cultivation 36h under 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described fold candida bacterium and Candida valida bacterium:
(1) composition of culture medium: yeast extract 4g, peptone 6g, glucose 6g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida bacteria culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that fold candida bacteria culture fluid and Candida valida bacteria culture fluid are housed respectively with fold candida bacterium and Candida valida bacterium on inoculating loop picking culture dish, carry out shaken cultivation 36h under 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Co-158 strain and Rhodopseudomonas palustris:
(1) composition of culture medium: ammonium chloride 4g, dipotassium hydrogen phosphate 1g, magnesium chloride 0.4g, sodium chloride 4g, yeast extract 1g, magnesium sulfate 2g, sodium thiosulfate 0.6g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Co-158 strain culture fluid and Rhodopseudomonas palustris culture fluid are housed respectively with Co-158 strain and Rhodopseudomonas palustris on inoculating loop picking culture dish, carry out shaken cultivation 36h under 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain:
(1) composition of culture medium: sucrose 30g, peptone 15g, magnesium sulfate 1g, potassium chloride 1g, ferrous sulfate 0.03g, dipotassium hydrogen phosphate 2g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Geotrichum Suaveolens culture fluid, excellent inulinase culture fluid, rough spore inulinase culture fluid and styreptomyces globispotus strain culture fluid are housed respectively with Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain on inoculating loop picking culture dish, carry out shaken cultivation 36h under 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of the special deodorizer of a kind of livestock and poultry farm is as follows:
The liquid culture of each bacterium is added in poultry deodorizer special culture solution, make each bacterium reach above-mentioned strain concentration, mix homogeneously;
Described poultry deodorizer special culture solution is made up of following material: Carnis Bovis seu Bubali cream 15g, peptone 5g, maltose 5g, glucose 4g, sodium chloride 2g, potassium dihydrogen phosphate 2g, ferrous sulfate 4g, magnesium sulfate 2g, zinc sulfate 2g, distilled water 1000g.
The using method of the special deodorizer of a kind of livestock and poultry farm: every day is sooner or later each in poultry house sprays once, every day consumption 0.6kg/m
3.
Embodiment 3
The special deodorizer of a kind of livestock and poultry farm, is made up of following microorganism fungus kind: bacillus acidophilus 10
7individual/ml, Lactobacillus reuteri 10
8individual/ml, bacillus polymyxa 10
5individual/ml, bacillus lentus 10
7individual/ml, Bacillus coagulans 10
7individual/ml, enterococcus faecalis 10
5individual/ml, enterococcus faecalis 10
8individual/ml, fold candida bacterium 10
5individual/ml, Candida valida bacterium 10
5individual/ml, Co-158 strain 10
5individual/ml, Rhodopseudomonas palustris 10
7individual/ml, Geotrichum Suaveolens 10
5individual/ml, excellent inulinase 10
7individual/ml, rough spore inulinase 10
7individual/ml and styreptomyces globispotus strain 10
7individual/ml;
The preparation method of described bacillus acidophilus and Lactobacillus reuteri:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 9g, peptone 9g, yeast extract 5g, glucose 4g, Tween 80 0.5ml, sodium acetate 4g, sodium citrate 3g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 0.5g, manganese sulfate 0.25g, ferrous sulfate 0.15g, distilled water 1000g; All culture medium raw materials carry out sterilization treatment before use;
(2) fermentation culture: measure two parts and load in two test tubes by the culture medium 8ml that above-mentioned raw materials is formulated, aseptically, access in two test tubes with the bacillus acidophilus on inoculating loop respectively picking culture dish and Lactobacillus reuteri, shaken cultivation 10h under 33 DEG C of conditions, add the plant extract 0.6g through sterilization treatment afterwards, continue to cultivate 18h, obtain the culture of bacillus acidophilus and Lactobacillus reuteri;
The preparation method of described plant extract is: by Fructus Lycii and Fructus Mori 1:2 mixing in mass ratio, in containing the sodium chloride solution of 2%, soak 18h, soaking temperature is 36 DEG C, soaks and terminates rear filtration, get filtering residue; Be placed in drying baker by filtering residue and dry, make its moisture lower than 12%, bake out temperature is 60 DEG C, pulverizes, and crosses 60 mesh sieves, obtains the powder of Fructus Lycii and Fructus Mori; In powder, add the ethanol of 82%, at 65 DEG C of temperature, steaming and decocting 4h, gets filtrate, filtrate is concentrated, spraying dry, pulverizes, and crosses 60 mesh sieves, obtains plant extract;
The preparation method of described bacillus polymyxa, bacillus lentus and Bacillus coagulans:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 26g, peptone 6g, glucose 16g, sodium chloride 4g, sodium hydrogen phosphate 2.5g, magnesium chloride 0.3g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid are housed respectively by bacillus polymyxa, bacillus lentus and Bacillus coagulans on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described enterococcus faecalis and enterococcus faecalis:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 4g, peptone 12g, sodium chloride 4g, sucrose 9g, dipotassium hydrogen phosphate 1.5g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare enterococcus faecalis culture fluid and enterococcus faecalis culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that enterococcus faecalis culture fluid and enterococcus faecalis culture fluid are housed respectively with enterococcus faecalis and enterococcus faecalis on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described fold candida bacterium and Candida valida bacterium:
(1) composition of culture medium: yeast extract 3g, peptone 5g, glucose 5g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida bacteria culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that fold candida bacteria culture fluid and Candida valida bacteria culture fluid are housed respectively with fold candida bacterium and Candida valida bacterium on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Co-158 strain and Rhodopseudomonas palustris:
(1) composition of culture medium: ammonium chloride 3g, dipotassium hydrogen phosphate 0.6g, magnesium chloride 0.3g, sodium chloride 3g, yeast extract 0.6g, magnesium sulfate 1.5g, sodium thiosulfate 0.5g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Co-158 strain culture fluid and Rhodopseudomonas palustris culture fluid are housed respectively with Co-158 strain and Rhodopseudomonas palustris on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain:
(1) composition of culture medium: sucrose 26g, peptone 16g, magnesium sulfate 0.6g, potassium chloride 0.6g, ferrous sulfate 0.02g, dipotassium hydrogen phosphate 1.5g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Geotrichum Suaveolens culture fluid, excellent inulinase culture fluid, rough spore inulinase culture fluid and styreptomyces globispotus strain culture fluid are housed respectively with Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of the special deodorizer of a kind of livestock and poultry farm is as follows:
The liquid culture of each bacterium is added in poultry deodorizer special culture solution, make each bacterium reach above-mentioned strain concentration, mix homogeneously;
Described poultry deodorizer special culture solution is made up of following material: Carnis Bovis seu Bubali cream 12g, peptone 4g, maltose 4g, glucose 3g, sodium chloride 1.5g, potassium dihydrogen phosphate 1.5g, ferrous sulfate 3g, magnesium sulfate 1.5g, zinc sulfate 1.5g, distilled water 1000g.
The using method of the special deodorizer of a kind of livestock and poultry farm: every day is sooner or later each in poultry house sprays once, every day consumption 0.4kg/m
3.
Test effect: deodorizer living bacteria count of the present invention is many, good deodorization effect.Test in the plant of twins (group) limited company subordinate with the deodorizer of embodiment of the present invention 1-3 preparation, result shows NH in feces
3and H
2s burst size declines 55 ~ 75% and 30 ~ 40% respectively, wherein the deodorizer best results prepared of embodiment 3, NH in feces
3and H
2s burst size declines 75% and 40% respectively.
Claims (8)
1. the special deodorizer of livestock and poultry farm, is characterized in that being made up of following microorganism fungus kind: bacillus acidophilus 10
6~ 10
8individual/ml, Lactobacillus reuteri 10
7~ 10
9individual/ml, bacillus polymyxa 10
4~ 10
6individual/ml, bacillus lentus 10
6~ 10
8individual/ml, Bacillus coagulans 10
6~ 10
9individual/ml, enterococcus faecalis 10
4~ 10
6individual/ml, enterococcus faecalis 10
7~ 10
9individual/ml, fold candida bacterium 10
4~ 10
7individual/ml, Candida valida bacterium 10
4~ 10
7individual/ml, Co-158 strain 10
4~ 10
6individual/ml, Rhodopseudomonas palustris 10
6~ 10
8individual/ml, Geotrichum Suaveolens 10
4~ 10
6individual/ml, excellent inulinase 10
6~ 10
8individual/ml, rough spore inulinase 10
6~ 10
8individual/ml and styreptomyces globispotus strain 10
6~ 10
9individual/ml.
2. the special deodorizer of a kind of livestock and poultry farm according to claim 1, is characterized in that being made up of following microorganism fungus kind: bacillus acidophilus 10
7individual/ml, Lactobacillus reuteri 10
8individual/ml, bacillus polymyxa 10
5individual/ml, bacillus lentus 10
7individual/ml, Bacillus coagulans 10
7individual/ml, enterococcus faecalis 10
5individual/ml, enterococcus faecalis 10
8individual/ml, fold candida bacterium 10
5individual/ml, Candida valida bacterium 10
5individual/ml, Co-158 strain 10
5individual/ml, Rhodopseudomonas palustris 10
7individual/ml, Geotrichum Suaveolens 10
5individual/ml, excellent inulinase 10
7individual/ml, rough spore inulinase 10
7individual/ml and styreptomyces globispotus strain 10
7individual/ml.
3. the special deodorizer of a kind of livestock and poultry farm according to claim 1-2, is characterized in that the preparation method of each bacterium:
The preparation method of described bacillus acidophilus and Lactobacillus reuteri:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 8 ~ 10g, peptone 8 ~ 10g, yeast extract 4 ~ 6g, glucose 3 ~ 5g, Tween 80 0.5ml, sodium acetate 3 ~ 5g, sodium citrate 2 ~ 4g, dipotassium hydrogen phosphate 1 ~ 2g, magnesium sulfate 0.4 ~ 0.6g, manganese sulfate 0.2 ~ 0.3g, ferrous sulfate 0.1 ~ 0.2g, distilled water 1000g; All culture medium raw materials carry out sterilization treatment before use;
(2) fermentation culture: measure two parts and load in two test tubes by culture medium 5 ~ 10ml that above-mentioned raw materials is formulated, aseptically, access in two test tubes with the bacillus acidophilus on inoculating loop respectively picking culture dish and Lactobacillus reuteri, shaken cultivation 6 ~ 12h under 30 ~ 35 DEG C of conditions, add the plant extract 0.5 ~ 1g through sterilization treatment afterwards, continue cultivation 12 ~ 24h, obtain the culture of bacillus acidophilus and Lactobacillus reuteri;
The preparation method of described plant extract is: by Fructus Lycii and Fructus Mori 1:2 mixing in mass ratio, in containing the sodium chloride solution of 1 ~ 3%, soak 12 ~ 24h, soaking temperature is 35 ~ 37 DEG C, soaks and terminates rear filtration, get filtering residue; Be placed in drying baker by filtering residue and dry, make its moisture lower than 12%, bake out temperature is 55 ~ 65 DEG C, pulverizes, and crosses 60 mesh sieves, obtains the powder of Fructus Lycii and Fructus Mori; In powder, add the ethanol of 80 ~ 85%, at 60 ~ 70 DEG C of temperature, steaming and decocting 3 ~ 5h, gets filtrate, filtrate is concentrated, spraying dry, pulverizes, and crosses 60 mesh sieves, obtains plant extract;
The preparation method of described bacillus polymyxa, bacillus lentus and Bacillus coagulans:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 25 ~ 30g, peptone 5 ~ 8g, glucose 15 ~ 20g, sodium chloride 3 ~ 5g, sodium hydrogen phosphate 2 ~ 3g, magnesium chloride 0.2 ~ 0.4g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid are housed respectively by bacillus polymyxa, bacillus lentus and Bacillus coagulans on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described enterococcus faecalis and enterococcus faecalis:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 3 ~ 5g, peptone 10 ~ 15g, sodium chloride 3 ~ 5g, sucrose 8 ~ 10g, dipotassium hydrogen phosphate 1 ~ 2g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare enterococcus faecalis culture fluid and enterococcus faecalis culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that enterococcus faecalis culture fluid and enterococcus faecalis culture fluid are housed respectively with enterococcus faecalis and enterococcus faecalis on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described fold candida bacterium and Candida valida bacterium:
(1) composition of culture medium: yeast extract 2 ~ 4g, peptone 4 ~ 6g, glucose 3 ~ 6g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida bacteria culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that fold candida bacteria culture fluid and Candida valida bacteria culture fluid are housed respectively with fold candida bacterium and Candida valida bacterium on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Co-158 strain and Rhodopseudomonas palustris:
(1) composition of culture medium: ammonium chloride 2 ~ 4g, dipotassium hydrogen phosphate 0.5 ~ 1g, magnesium chloride 0.2 ~ 0.4g, sodium chloride 2 ~ 4g, yeast extract 0.5 ~ 1g, magnesium sulfate 1 ~ 2g, sodium thiosulfate 0.4 ~ 0.6g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Co-158 strain culture fluid and Rhodopseudomonas palustris culture fluid are housed respectively with Co-158 strain and Rhodopseudomonas palustris on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain:
(1) composition of culture medium: sucrose 25 ~ 30g, peptone 10 ~ 15g, magnesium sulfate 0.5 ~ 1g, potassium chloride 0.5 ~ 1g, ferrous sulfate 0.01 ~ 0.03g, dipotassium hydrogen phosphate 1 ~ 2g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Geotrichum Suaveolens culture fluid, excellent inulinase culture fluid, rough spore inulinase culture fluid and styreptomyces globispotus strain culture fluid are housed respectively with Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain on inoculating loop picking culture dish, carry out shaken cultivation 24 ~ 36h under 26 ~ 37 DEG C of conditions, obtain the liquid culture of each bacterium respectively.
4. the special deodorizer of a kind of livestock and poultry farm according to claim 3, is characterized in that the preparation method of each bacterium:
The preparation method of described bacillus acidophilus and Lactobacillus reuteri:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 9g, peptone 9g, yeast extract 5g, glucose 4g, Tween 80 0.5ml, sodium acetate 4g, sodium citrate 3g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 0.5g, manganese sulfate 0.25g, ferrous sulfate 0.15g, distilled water 1000g; All culture medium raw materials carry out sterilization treatment before use;
(2) fermentation culture: measure two parts and load in two test tubes by the culture medium 8ml that above-mentioned raw materials is formulated, aseptically, access in two test tubes with the bacillus acidophilus on inoculating loop respectively picking culture dish and Lactobacillus reuteri, shaken cultivation 10h under 33 DEG C of conditions, add the plant extract 0.6g through sterilization treatment afterwards, continue to cultivate 18h, obtain the culture of bacillus acidophilus and Lactobacillus reuteri;
The preparation method of described plant extract is: by Fructus Lycii and Fructus Mori 1:2 mixing in mass ratio, in containing the sodium chloride solution of 2%, soak 18h, soaking temperature is 36 DEG C, soaks and terminates rear filtration, get filtering residue; Be placed in drying baker by filtering residue and dry, make its moisture lower than 12%, bake out temperature is 60 DEG C, pulverizes, and crosses 60 mesh sieves, obtains the powder of Fructus Lycii and Fructus Mori; In powder, add the ethanol of 82%, at 65 DEG C of temperature, steaming and decocting 4h, gets filtrate, filtrate is concentrated, spraying dry, pulverizes, and crosses 60 mesh sieves, obtains plant extract;
The preparation method of described bacillus polymyxa, bacillus lentus and Bacillus coagulans:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 26g, peptone 6g, glucose 16g, sodium chloride 4g, sodium hydrogen phosphate 2.5g, magnesium chloride 0.3g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that bacillus polymyxa culture fluid, bacillus lentus culture fluid and Bacillus coagulans culture fluid are housed respectively by bacillus polymyxa, bacillus lentus and Bacillus coagulans on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described enterococcus faecalis and enterococcus faecalis:
(1) composition of culture medium: Carnis Bovis seu Bubali cream 4g, peptone 12g, sodium chloride 4g, sucrose 9g, dipotassium hydrogen phosphate 1.5g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare enterococcus faecalis culture fluid and enterococcus faecalis culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that enterococcus faecalis culture fluid and enterococcus faecalis culture fluid are housed respectively with enterococcus faecalis and enterococcus faecalis on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described fold candida bacterium and Candida valida bacterium:
(1) composition of culture medium: yeast extract 3g, peptone 5g, glucose 5g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida bacteria culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that fold candida bacteria culture fluid and Candida valida bacteria culture fluid are housed respectively with fold candida bacterium and Candida valida bacterium on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Co-158 strain and Rhodopseudomonas palustris:
(1) composition of culture medium: ammonium chloride 3g, dipotassium hydrogen phosphate 0.6g, magnesium chloride 0.3g, sodium chloride 3g, yeast extract 0.6g, magnesium sulfate 1.5g, sodium thiosulfate 0.5g, distilled water 1000g form;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Co-158 strain culture fluid and Rhodopseudomonas palustris culture fluid are housed respectively with Co-158 strain and Rhodopseudomonas palustris on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively;
The preparation method of described Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain:
(1) composition of culture medium: sucrose 26g, peptone 16g, magnesium sulfate 0.6g, potassium chloride 0.6g, ferrous sulfate 0.02g, dipotassium hydrogen phosphate 1.5g, distilled water 1000g;
(2) fermentation culture: take above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain culture fluid, be distributed into test tube, often pipe 5ml, sterilizing saves backup; Aseptically, access in the test tube that Geotrichum Suaveolens culture fluid, excellent inulinase culture fluid, rough spore inulinase culture fluid and styreptomyces globispotus strain culture fluid are housed respectively with Geotrichum Suaveolens, excellent inulinase, rough spore inulinase and styreptomyces globispotus strain on inoculating loop picking culture dish, carry out shaken cultivation 30h under 30 DEG C of conditions, obtain the liquid culture of each bacterium respectively.
5. the special deodorizer of a kind of livestock and poultry farm according to claim 1-2, is characterized in that preparation method is as follows:
The liquid culture of each bacterium is added in poultry deodorizer special culture solution, make each bacterium reach above-mentioned strain concentration, mix homogeneously;
Described poultry deodorizer special culture solution is made up of following material: Carnis Bovis seu Bubali cream 10 ~ 15g, peptone 3 ~ 5g, maltose 3 ~ 5g, glucose 2 ~ 4g, sodium chloride 1 ~ 2g, potassium dihydrogen phosphate 1 ~ 2g, ferrous sulfate 2 ~ 4g, magnesium sulfate 1 ~ 2g, zinc sulfate 1 ~ 2g, distilled water 1000g.
6. the special deodorizer of a kind of livestock and poultry farm according to claim 5, is characterized in that preparation method is as follows:
The liquid culture of each bacterium is added in poultry deodorizer special culture solution, make each bacterium reach above-mentioned strain concentration, mix homogeneously;
Described poultry deodorizer special culture solution is made up of following material: Carnis Bovis seu Bubali cream 12g, peptone 4g, maltose 4g, glucose 3g, sodium chloride 1.5g, potassium dihydrogen phosphate 1.5g, ferrous sulfate 3g, magnesium sulfate 1.5g, zinc sulfate 1.5g, distilled water 1000g.
7. the special deodorizer of a kind of livestock and poultry farm according to claim 1-2, is characterized in that using method: every day is sooner or later each in poultry house sprays once, every day consumption 0.3 ~ 0.6kg/m
3.
8. the special deodorizer of a kind of livestock and poultry farm according to claim 7, is characterized in that using method: every day is sooner or later each in poultry house sprays once, every day consumption 0.4kg/m
3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410615491.0A CN104307012B (en) | 2014-11-05 | 2014-11-05 | A kind of special deodorant of livestock and poultry farm and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410615491.0A CN104307012B (en) | 2014-11-05 | 2014-11-05 | A kind of special deodorant of livestock and poultry farm and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104307012A true CN104307012A (en) | 2015-01-28 |
| CN104307012B CN104307012B (en) | 2017-08-11 |
Family
ID=52362413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410615491.0A Active CN104307012B (en) | 2014-11-05 | 2014-11-05 | A kind of special deodorant of livestock and poultry farm and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104307012B (en) |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940968A (en) * | 2015-07-23 | 2015-09-30 | 成都千方百剂科技有限公司 | Biological air freshener and preparation method thereof |
| CN105477671A (en) * | 2015-12-22 | 2016-04-13 | 湖南普泰尔微科环保科技有限公司 | Deodorizing method by matching microbial deodorizer with fog gun equipment |
| CN105497958A (en) * | 2015-12-22 | 2016-04-20 | 湖南普泰尔微科环保科技有限公司 | Method for deodorization through plant deodorant with high-pressure sprayer |
| CN105557771A (en) * | 2015-12-11 | 2016-05-11 | 安徽安泰农业开发有限责任公司 | Preparation method of spraying medicine for preventing encephalitis B of boar |
| CN106244481A (en) * | 2016-07-29 | 2016-12-21 | 河南普爱饲料股份有限公司 | A kind of compound micro-ecological preparation, its preparation method and application processed for farm animal excrement |
| CN107118995A (en) * | 2017-06-29 | 2017-09-01 | 重庆市万源禽蛋食品有限公司 | Microbial fermentation inoculum and its application and apply its livestock manure fermented method |
| CN107299068A (en) * | 2017-05-17 | 2017-10-27 | 刘宇 | A kind of swine excrement deodorant and preparation method thereof |
| CN107345214A (en) * | 2017-06-27 | 2017-11-14 | 江苏远山生物技术有限公司 | Useless blood deodorant of a kind of composite efficient microorganism cattle and sheep and preparation method thereof |
| CN107500884A (en) * | 2017-09-11 | 2017-12-22 | 大连三仪动物药品有限公司 | Compound formulation for fermented livestock excrement production biological organic fertilizer |
| CN108040888A (en) * | 2017-12-11 | 2018-05-18 | 嘉兴佳康丽生物科技有限公司 | A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method |
| CN108499353A (en) * | 2018-04-04 | 2018-09-07 | 江西万年鑫星农牧股份有限公司 | A kind of special deodorant in scale pig farm and its application |
| CN108531412A (en) * | 2018-03-20 | 2018-09-14 | 大连三净集团有限公司 | A kind of preparation method of ECMD Efficient Rings control microbial deodorant |
| CN109370934A (en) * | 2018-10-15 | 2019-02-22 | 上海国龙生物科技有限公司 | A kind of aquaculture wastewater processing microbial bacterial agent |
| CN109464375A (en) * | 2018-12-11 | 2019-03-15 | 深圳合民生物科技有限公司 | A kind of pet deodorant and its preparation method and application |
| CN109609394A (en) * | 2018-12-26 | 2019-04-12 | 大连雪龙都市农业有限公司 | A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof |
| WO2019092134A1 (en) | 2017-11-08 | 2019-05-16 | Nolivade | Composition for improving the well-being of animals |
| CN109837229A (en) * | 2019-04-11 | 2019-06-04 | 河北维尔利动物药业集团有限公司 | The preparation method of clostridium butyricum composite bacteria liquid for poultry |
| CN109988730A (en) * | 2019-04-09 | 2019-07-09 | 华南农业大学 | A kind of biocontrol microorganisms of mulberry tree disease and its application |
| WO2020004580A1 (en) * | 2018-06-28 | 2020-01-02 | 一丸ファルコス株式会社 | Body odor production-preventing agent containing lactic acid bacterium |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN111254096A (en) * | 2020-02-28 | 2020-06-09 | 山东睿智医药科技有限公司 | Method for improving indoor environmental sanitation |
| CN111821847A (en) * | 2020-07-28 | 2020-10-27 | 湖南艾凯环保科技有限公司 | Composite biological deodorant and preparation method thereof |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102688516B (en) * | 2011-11-30 | 2013-12-25 | 河南科技大学 | Deodorizing and dedusting agent for chicken farm and preparation method thereof |
-
2014
- 2014-11-05 CN CN201410615491.0A patent/CN104307012B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102688516B (en) * | 2011-11-30 | 2013-12-25 | 河南科技大学 | Deodorizing and dedusting agent for chicken farm and preparation method thereof |
Cited By (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940968A (en) * | 2015-07-23 | 2015-09-30 | 成都千方百剂科技有限公司 | Biological air freshener and preparation method thereof |
| CN105557771A (en) * | 2015-12-11 | 2016-05-11 | 安徽安泰农业开发有限责任公司 | Preparation method of spraying medicine for preventing encephalitis B of boar |
| CN105477671B (en) * | 2015-12-22 | 2017-12-08 | 湖南普泰尔环境股份有限公司 | A kind of microbial deodorant coordinates the deodorizing method of fog gun equipment |
| CN105477671A (en) * | 2015-12-22 | 2016-04-13 | 湖南普泰尔微科环保科技有限公司 | Deodorizing method by matching microbial deodorizer with fog gun equipment |
| CN105497958A (en) * | 2015-12-22 | 2016-04-20 | 湖南普泰尔微科环保科技有限公司 | Method for deodorization through plant deodorant with high-pressure sprayer |
| CN105497958B (en) * | 2015-12-22 | 2017-12-29 | 湖南普泰尔环境股份有限公司 | A kind of plant deodorant coordinates the deodorizing method of high pressure spraying machine |
| CN106244481A (en) * | 2016-07-29 | 2016-12-21 | 河南普爱饲料股份有限公司 | A kind of compound micro-ecological preparation, its preparation method and application processed for farm animal excrement |
| CN107299068A (en) * | 2017-05-17 | 2017-10-27 | 刘宇 | A kind of swine excrement deodorant and preparation method thereof |
| CN107345214A (en) * | 2017-06-27 | 2017-11-14 | 江苏远山生物技术有限公司 | Useless blood deodorant of a kind of composite efficient microorganism cattle and sheep and preparation method thereof |
| CN107118995A (en) * | 2017-06-29 | 2017-09-01 | 重庆市万源禽蛋食品有限公司 | Microbial fermentation inoculum and its application and apply its livestock manure fermented method |
| CN107118995B (en) * | 2017-06-29 | 2019-08-23 | 重庆市万源禽蛋食品有限公司 | Microbial fermentation inoculum and its application and the livestock manure fermented method for applying it |
| CN107500884A (en) * | 2017-09-11 | 2017-12-22 | 大连三仪动物药品有限公司 | Compound formulation for fermented livestock excrement production biological organic fertilizer |
| WO2019092134A1 (en) | 2017-11-08 | 2019-05-16 | Nolivade | Composition for improving the well-being of animals |
| CN108040888A (en) * | 2017-12-11 | 2018-05-18 | 嘉兴佳康丽生物科技有限公司 | A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method |
| CN108531412A (en) * | 2018-03-20 | 2018-09-14 | 大连三净集团有限公司 | A kind of preparation method of ECMD Efficient Rings control microbial deodorant |
| CN108499353A (en) * | 2018-04-04 | 2018-09-07 | 江西万年鑫星农牧股份有限公司 | A kind of special deodorant in scale pig farm and its application |
| CN108499353B (en) * | 2018-04-04 | 2021-01-01 | 江西万年鑫星农牧股份有限公司 | Special deodorant for large-scale pig farm and application of special deodorant |
| WO2020004580A1 (en) * | 2018-06-28 | 2020-01-02 | 一丸ファルコス株式会社 | Body odor production-preventing agent containing lactic acid bacterium |
| JPWO2020004580A1 (en) * | 2018-06-28 | 2021-08-05 | 一丸ファルコス株式会社 | Body odor generation inhibitor containing lactic acid bacteria |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN109370934A (en) * | 2018-10-15 | 2019-02-22 | 上海国龙生物科技有限公司 | A kind of aquaculture wastewater processing microbial bacterial agent |
| CN109370934B (en) * | 2018-10-15 | 2021-11-26 | 上海国龙生物科技有限公司 | Microbial agent for treating aquaculture sewage |
| CN109464375A (en) * | 2018-12-11 | 2019-03-15 | 深圳合民生物科技有限公司 | A kind of pet deodorant and its preparation method and application |
| CN109609394A (en) * | 2018-12-26 | 2019-04-12 | 大连雪龙都市农业有限公司 | A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof |
| CN109988730A (en) * | 2019-04-09 | 2019-07-09 | 华南农业大学 | A kind of biocontrol microorganisms of mulberry tree disease and its application |
| CN109837229A (en) * | 2019-04-11 | 2019-06-04 | 河北维尔利动物药业集团有限公司 | The preparation method of clostridium butyricum composite bacteria liquid for poultry |
| CN111254096A (en) * | 2020-02-28 | 2020-06-09 | 山东睿智医药科技有限公司 | Method for improving indoor environmental sanitation |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
| CN111821847A (en) * | 2020-07-28 | 2020-10-27 | 湖南艾凯环保科技有限公司 | Composite biological deodorant and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104307012B (en) | 2017-08-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104307012A (en) | Deodorant special for livestock and poultry farms and application thereof | |
| CN102247611B (en) | Microorganism deodorant and preparation method thereof | |
| CN100375637C (en) | Biological deodorization and purification agent, and its use | |
| CN104667320B (en) | Composite microbial deodorant for treating household garbage and preparation method of deodorant | |
| CN107189972A (en) | Microbial deodorant, preparation method and the usage | |
| CN106268287A (en) | A kind of new method reducing plant's foul gas generation | |
| CN104388363A (en) | Compound bacteria for organic refuse deodorization and reduction and preparation method thereof | |
| CN103031253A (en) | Novel compound microorganism living bacteria agent, method for preparing same and applications | |
| CN101711885A (en) | Method for preparing high-concentration biological deodorant | |
| CN102813948A (en) | Preparation method of composite biological deodorant | |
| CN106967643A (en) | A kind of Novel compound microbial agent and preparation method thereof | |
| CN103977704A (en) | Method for deodorizing garbage by utilizing composite microbial agent | |
| KR101807242B1 (en) | A composition for removing odorous materials | |
| CN103468619A (en) | Microbial preparation for purifying air | |
| CN109364737A (en) | A kind of compound deodorizer | |
| CN103357056A (en) | Healthy biological spraying agent for removing peculiar smell and preparation method thereof | |
| CN108913634A (en) | A kind of dystopy processing method of fowl and animal excrement and its complex microorganism, the dystopy fermenting bed padding used | |
| CN109576169A (en) | A kind of microbial deodorant and preparation method thereof | |
| CN106809957A (en) | A kind of plants probiotics deodorant | |
| CN105585235A (en) | Compound microbial preparation with deodorization function, preparation method and application | |
| CN108148790A (en) | A kind of mixed bacteria, carrier and the method for livestock and poultry biology dejection degradation | |
| CN110024701A (en) | A kind of ecologically raising pigs fermentation bed and its management method | |
| CN109331641A (en) | A kind of preparation method of garbage deodorant | |
| CN108947164A (en) | A kind of application method of complex microorganism preparations | |
| CN106995788A (en) | A kind of complex microorganism preparations and preparation method and application for decomposing human feces |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Jianxi Inventor after: Wu Zhiqing Inventor after: Zhao Yanping Inventor before: Wang Yongfei Inventor before: Wu Zhiqing Inventor before: Zhao Yanping |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |