CN103357056A - Healthy biological spraying agent for removing peculiar smell and preparation method thereof - Google Patents
Healthy biological spraying agent for removing peculiar smell and preparation method thereof Download PDFInfo
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- CN103357056A CN103357056A CN2013103100399A CN201310310039A CN103357056A CN 103357056 A CN103357056 A CN 103357056A CN 2013103100399 A CN2013103100399 A CN 2013103100399A CN 201310310039 A CN201310310039 A CN 201310310039A CN 103357056 A CN103357056 A CN 103357056A
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- bacillus subtilis
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Abstract
The invention provides a healthy spraying agent for removing peculiar smell. The healthy spraying agent comprises the following raw materials: by weight, 10-20% of bacillus subtilis, 20-30% of lactobacillus plantarum, and 50-70% of Rhodopseudomonas palustris. The invention also provides a method of the above spraying agent, comprising the following steps: Step 1, providing a bacillus subtilis strain culture solution; Step 2, providing a Rhodopseudomonas palustris strain culture solution; and Step 3, mixing bacillus subtilis and Rhodopseudomonas palustris to obtain the spraying agent. According to the preparation method of the healthy biological spraying agent for removing peculiar smell in a house kitchen and a sewer of a toilet, effects of compound microorganisms can be utilized to decompose organic matters in sewage and bad smell. By the use of the spraying agent, organic matters in kitchen or a sewer of a toilet can be decomposed, peculiar smell is minimized within a short period, environmental health is improved, growth of harmful bacteria in sewage can be inhibited, breeding of pathogenic bacteria is inhibited, and diseases are prevented.
Description
Technical field
The present invention relates to environmental technology field, relate in particular to a kind of biological spray of health type of removing abnormal flavour and preparation method thereof.
Background technology
Can there be the sanitary sewage of scrubbing more or less every day in each family, can be drained by the most sewage of flushing cleaning, but also has a fraction of sewage, and dirt is stayed lower water pipe bent position, and long-term accumulation month after month produces abnormal flavour easily.Toilet and kitchen often can emit bad smell, and especially after the rain, it is more dense that this taste becomes, and directly has influence on our daily life.A lot of nifves all are from sewer in fact.If do not remove these unpleasant tastes, the one, can pollute indoor environment, another can cause such as insects such as Blatta seu periplaneta, damp worms.So how to remove these abnormal flavour, become many housewives' in summer a difficult problem.
Present home-use deodorant products, main chemical classes product is main, main component such as maleic acid, ferrous chloride, photocatalyst etc.The agent of a kind of multipurpose high-efficiency environment-friendly deodorant is disclosed such as patent CN102206556A, made by potassium carbonate, sodium tetraborate, sodium bicarbonate, sodium lauryl sulphate, it is characterized in that: the weight proportion of each component is: potassium carbonate 10-30kg, sodium tetraborate 20-60Kg, sodium bicarbonate 20-50Kg, sodium lauryl sulphate 3-8kg are applicable to the various places that the stinks such as kitchen, room, factory, hospital, lavatory, sewage treatment plant, destructor plant pollute.Patent CN102526784A discloses a kind of deodorant for toilet method for making for another example, and component is C11-C14 sodium alkyl benzene sulfonate, hydrochloric acid, ferrous chloride, zinc chloride, aluminum chloride, sodium chloride, magnesium chloride, lignin, kieselguhr, water.
Yet chemical deodorizing has certain limitation, and every kind of chemical products all is product with strong points, when meeting other complicated chemical matrix, just can lose efficacy; Use after the chemical products, chemical residue is always arranged in water body, it may bring side effect or new pollution; But use the chemical products mask malodors, but can not change the generation of stink or stop it to distribute.
Summary of the invention
For some limitation of chemical deodorizing, all be product with strong points such as every kind of chemical products, when meeting other complicated chemical matrix, just can lose efficacy; Use after the chemical products, chemical residue is always arranged in water body, it may bring side effect or new pollution; But use the chemical products mask malodors, but can not change the generation of stink or stop the problems such as it distributes, the invention provides a kind of biological spray of health type of removing abnormal flavour and preparation method thereof, it comprises following technical scheme:
First aspect of the present invention provides a kind of biological spray of health type of removing abnormal flavour, and according to percentage by weight, raw material comprises:
Bacillus subtilis (Bacillus subtilis) 10-20wt%;
Lactobacillus plantarum (Lactobacillus plantarum) 20-30wt%;
Rhodopseudomonas palustris (Rhodopseudomonas palustris) 50-70wt%.
The biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, can also contain the microorganism that other can be used for removing abnormal flavour, such as bacillus acidophilus, saccharomyces cerevisiae etc., form but be preferably by bacillus subtilis, Lactobacillus plantarum and Rhodopseudomonas palustris, and do not comprise other strains.
Wherein, take the gross weight of described spray as benchmark, the percentage by weight of bacillus subtilis, Lactobacillus plantarum and Rhodopseudomonas palustris is respectively 10～20wt%, 20-30wt%, 50～70wt%, is preferably 15-26wt%, 22-32wt%, 40-65wt%.
The biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, spray can also contain water, take the gross weight of spray as benchmark, the percentage by weight 0～20wt% of water.
The biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, preferably, the content of microbial inoculum reaches respectively, bacillus subtilis 0.5-1 * 10
10Cfu/ml, Lactobacillus plantarum 1-5 * 10
8Cfu/ml, Rhodopseudomonas palustris 1-2 * 10
9Cfu/ml.
Second aspect of the present invention provides a kind of method for preparing the biological spray of health type of above-mentioned removing abnormal flavour, and described method comprises the steps:
Step 1 provides the Bacillus subtilis strain culture fluid;
Step 2 provides the Rhodopseudomonas palustris bacteria culture fluid;
Step 3 is mixed bacillus subtilis, Rhodopseudomonas palustris, obtains described spray.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 1, the raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 1-3wt%, culture medium 97-99wt%, at a certain temperature, carry out aerobic fermentation.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 1, the fermentation time of bacillus subtilis is preferably 1-5 days, more preferably 1-4 days, most preferably is 1-3 days.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 1, in the sweat of described bacillus subtilis, temperature is preferably arbitrary temp or the temperature range that is controlled in the 20-40 ° of C scope.
The used culture medium matrix component of incubation is preferably at least two kinds of mixture of bean cake powder, starch, yeast powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, sterilized water, and its percentage by weight is preferably: bean cake powder 1.5-30wt%, Semen Maydis powder 2-40wt%, starch 0-15wt%, yeast powder 0-10wt%, peptone 0-5wt%, molasses 0-15wt%, sodium hydrogen phosphate 0-2wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-3wt%, water 10-99wt%.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 2, the raw material of anaerobism illumination fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 5-10wt%, culture medium 90-95wt%, at a certain temperature, carry out anaerobism illumination fermentation.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 2, the fermentation time of Rhodopseudomonas palustris is 5-10 days, more preferably 6-9 days, most preferably is 6-8 days.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 2, in the sweat of described Rhodopseudomonas palustris, temperature is preferably arbitrary temp or the temperature range that is controlled in the 20-40 ° of C scope.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 2, the used culture medium matrix component of incubation is preferably at least two kinds of mixture of yeast powder, peptone, sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its weight percent proportioning is: yeast powder 0-1wt%, peptone 0-1wt%, sodium acetate 0-3wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-1wt%, water 90-99wt%.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 3, lactobacillus bacterium liquid adopts conventional microorganism culturing means to carry out liquid culture, medium optimization is MRS, and temperature is preferably arbitrary temp or the temperature range that is controlled in the 20-40 ° of C scope, and incubation time is preferably anaerobism and cultivated 1-5 days, more preferably 2-4 days, most preferably be 3 days.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 3, with bacillus subtilis, Lactobacillus plantarum, Rhodopseudomonas palustris and water, 10～20wt%, 20-30wt%, 50-70wt%, 0-20wt% mix by weight percentage.
The preparation method of the biological spray of the health type of the removing abnormal flavour that the present invention is above-mentioned, wherein, in the step 3, preferably, the content of microbial inoculum reaches respectively, bacillus subtilis 0.5-1 * 10
10Cfu/ml, Lactobacillus plantarum 1-5 * 10
8Cfu/ml, Rhodopseudomonas palustris 1-2 * 10
9Cfu/ml, preferably, the bacterium amount of each bacterium is carried out regulating and controlling by centrifugal concentrating or interpolation water.
Above-mentioned technical scheme has following advantage or beneficial effect:
The preparation method of the biological spray of the health type of removing abnormal flavour provided by the invention can be utilized the effect of complex microorganism, and to the Organic substance in the sewage, foul smell decomposes, and reaches the effect of abnormal flavour and harmless treatment.Both can decompose the Organic substance in kitchen or the lavatory drain, reduce in a short time abnormal flavour, improve environmental sanitation, suppress again the growth of the harmful bacteria in the sewage, suppress the breeding of pathogenic antibacterial, prevent the generation of disease.
The specific embodiment
Below describe several preferred embodiments of the present invention in detail:
In following examples, used strain is:
Described bacillus subtilis is the preservation of Chinese agriculture microorganism fungus kind preservation administrative center, strains A CCC10619; Described Rhodopseudomonas palustris is the preservation of Chinese agriculture microorganism fungus kind preservation administrative center, bacterial strain ACCC10649; Described Lactobacillus plantarum, the preservation of Chinese agriculture microorganism fungus kind preservation administrative center, bacterial strain ACCC11118.
But those skilled in the art can be understood that, the content of description according to the present invention also can adopt other available bacillus subtilis, Lactobacillus plantarum, Rhodopseudomonas palustris to be prepared.Therefore, above-mentioned specific bacterial strain can not be interpreted as limitation of the scope of the invention.
Embodiment 1
Raw material forms:
Bacillus subtilis 10wt%;
Lactobacillus plantarum 25wt%;
Rhodopseudomonas palustris 60wt%;
Water 5wt%
Preparation method:
Step 1:
The raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 1wt%, culture medium 99wt%, carried out 30 ℃ of aerobic fermentations 2 days.Wherein, described culture medium is, by weight percentage, the mixture of bean cake powder 3wt%, Semen Maydis powder 2wt%, starch 0wt%, yeast powder 0.5wt%, peptone 0.5wt%, molasses 0.5wt%, sodium hydrogen phosphate 0.5wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, water 92.9wt%.
Step 2,
The raw material of anaerobic fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 10wt%, culture medium 90wt%, carry out 30 ℃ of anaerobism illumination fermentations 7 days.Described culture medium is to be by weight percentage the mixture of yeast powder 1wt%, peptone 0wt%, sodium acetate 3wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, water 95.9wt%.
Step 3,
Adopt conventional microorganism culturing means to carry out liquid culture Lactobacillus plantarum bacterium liquid, culture medium is MRS, and incubation time is that 30 ℃ of anaerobism were cultivated 3 days.Again bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, water are carried out the composite spray that makes take the gross weight of spray as benchmark by following bacteria containing amount and weight ratio, bacillus subtilis 10wt%, Lactobacillus plantarum 25wt%, Rhodopseudomonas palustris 60wt%, water 5wt%, wherein, bacillus subtilis bacterium amount reaches 1 * 10
10Cfu/ml, Lactobacillus plantarum bacterium amount reach 1 * 10
8Cfu/ml, Rhodopseudomonas palustris bacterium amount reach 1 * 10
9Cfu/ml.
Embodiment 2
Raw material forms:
Bacillus subtilis 20wt%;
Lactobacillus plantarum 25wt%;
Rhodopseudomonas palustris 50wt%;
Water 5wt%
Preparation method:
Step 1:
The raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 3wt%, culture medium 97wt%, carried out 30 ℃ of aerobic fermentations 2 days.Wherein, described culture medium is, by weight percentage, the mixture of bean cake powder 3.5wt%, Semen Maydis powder 2.5wt%, starch 0wt%, yeast powder 0wt%, peptone 0.75wt%, molasses 1.0wt%, sodium hydrogen phosphate 0.3wt%, potassium dihydrogen phosphate 0.08wt%, magnesium sulfate 0wt%, water 91.87wt%.
Step 2,
The raw material of anaerobic fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 20wt%, culture medium 80wt%, carry out 30 ℃ of anaerobism illumination fermentations 7 days.Described culture medium is to be by weight percentage the mixture of yeast powder 1.5wt%, peptone 0.5wt%, sodium acetate 4wt%, potassium dihydrogen phosphate 0.05wt%, magnesium sulfate 0.05wt%, water 93.9wt%.
Step 3,
Adopt conventional microorganism culturing means to carry out liquid culture Lactobacillus plantarum bacterium liquid, culture medium is MRS, and incubation time is that 30 ℃ of anaerobism were cultivated 3 days.Again bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, water are carried out the composite spray that makes take the gross weight of spray as benchmark by following bacteria containing amount and weight ratio, bacillus subtilis 20wt%, Lactobacillus plantarum 25wt%, Rhodopseudomonas palustris 50wt%, water 5wt%, wherein, bacillus subtilis bacterium amount reaches 5 * 10
9Cfu/ml, Lactobacillus plantarum bacterium amount reach 2 * 10
8Cfu/ml, Rhodopseudomonas palustris bacterium amount reach 5 * 10
8Cfu/ml.
The deodorizing effect experiment:
Experimental example 1: get certain city's water channel sewage, this water channel is black, and distributes stench, and testing its BOD is 1250mg/L, gets the smelly sewage of 1L and is sprayed into the biological spray 1ml of the present invention, and after 4-8 hour, foul smell disappears, and obviously reduces by the subjective appreciation stench.
Experimental example 2: be 1mg/m at content of formaldehyde
3, area is 20m
2New decorating house in, spray the biological spray of 10ml, after 2-6 days, Detection of Air Quality content of formaldehyde≤0.10mg/m
3
Experimental example 3: be 5mg/m at ammonia level
3, area is 15m
2Pigsty in, spray the biological spray of 100ml, after 2-6 days, Detection of Air Quality ammonia level≤0.50mg/m
3
Embodiment 4: be 0.4mg/m at benzene content
3newly purchase in the automobile, spray the biological spray of 10ml, after 2-6 days, Detection of Air Quality benzene content≤0.05mg/m
3
The preparation method of the biological spray of the health type of removing abnormal flavour provided by the invention can be utilized the effect of complex microorganism, and the Organic substance in the sewage, foul smell are decomposed, and reaches the effect of abnormal flavour and harmless treatment.Both can decompose the Organic substance in kitchen or the lavatory drain, reduce in a short time abnormal flavour, improve environmental sanitation, suppress again the growth of the harmful bacteria in the sewage, suppress the breeding of pathogenic antibacterial, prevent the generation of disease.
More than specific embodiments of the invention are described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of doing under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (10)
1. biological spray of the health type of removing abnormal flavour is characterized in that according to percentage by weight, raw material comprises:
Bacillus subtilis 10-20wt%;
Lactobacillus plantarum 20-30wt%;
Rhodopseudomonas palustris 50-70wt%.
2. the biological spray of the health type of described removing abnormal flavour according to claim 1 is characterized in that, also comprises water, take the gross weight of spray as benchmark, and the percentage by weight 0-20wt% of water.
3. one kind prepares the as claimed in claim 1 method of spray, it is characterized in that, comprises following step:
Step 1 provides the Bacillus subtilis strain culture fluid;
Step 2 provides the Rhodopseudomonas palustris bacteria culture fluid;
Step 3 is mixed bacillus subtilis, Rhodopseudomonas palustris, obtains described spray.
4. method according to claim 3 is characterized in that, in the step 1, and the raw material of aerobic fermentation following weight percentage ratio: Bacillus subtilis strain 1-3wt%, culture medium 97-99wt%, at a certain temperature, carried out aerobic fermentation 1-5 days.
5. method according to claim 4, it is characterized in that, culture medium is at least two kinds of mixture for bean cake powder, starch, yeast powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, sterilized water, and its weight percent proportioning is: bean cake powder 1.5-30wt%, Semen Maydis powder 2-40wt%, starch 0-15wt%, yeast powder 0-10wt%, peptone 0-5wt%, molasses 0-15wt%, sodium hydrogen phosphate 0-2wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-3wt%, water 10-99wt%.
6. method according to claim 3, it is characterized in that, in the step 2, the raw material of anaerobism illumination fermentation following weight percentage ratio: Rhodopseudomonas palustris strain 5-10wtwt%, culture medium 90-95wt%, at a certain temperature, carry out anaerobism illumination fermentation 5-10 days.
7. method according to claim 6, it is characterized in that, culture medium is at least two kinds of mixture of yeast powder, peptone, sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its weight percent proportioning is: yeast powder 0-1wt%, peptone 0-1wt%, sodium acetate 0-3wt%, potassium dihydrogen phosphate 0-2wt%, magnesium sulfate 0-1wt%, water 90-99wt%.
8. according to claim 4 or 6 described methods, it is characterized in that described temperature is preferably arbitrary temp or the temperature range that is controlled in the 20-40 ° of C scope.
9. method according to claim 3 is characterized in that, in the step 3, with bacillus subtilis, Lactobacillus plantarum, Rhodopseudomonas palustris and water, 10-20wt%, 20-30wt%, 50-70wt%, 0-20wt% mix by weight percentage.
10. method according to claim 3 is characterized in that, in the step 3, the content of microbial inoculum reaches respectively, bacillus subtilis 0.5-1 * 10
10Cfu/ml, Lactobacillus plantarum 1-5 * 10
8Cfu/ml, Rhodopseudomonas palustris 1-2 * 10
9Cfu/ml.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104248911A (en) * | 2014-05-13 | 2014-12-31 | 江苏绿华生物工程有限公司 | Biological deodorant and preparation method thereof |
| CN106754520A (en) * | 2016-12-27 | 2017-05-31 | 上海创博生态工程有限公司 | It is a kind of to control to be harmful to biological agent of miscellaneous bacteria quantity and preparation method thereof in winter pig house |
| CN108165502A (en) * | 2017-12-15 | 2018-06-15 | 长沙理工大学 | One plant of lactobacillus plantarum for being used to improve fresh-cut lettuce flavor |
| CN109010166A (en) * | 2018-07-05 | 2018-12-18 | 上海普缇康生物技术有限公司 | A kind of plant source small molecule cleaning agent for mouth cavity and preparation method thereof |
| CN109293732A (en) * | 2018-10-23 | 2019-02-01 | 宣城市福贝宠物食品有限公司 | Method for removing peculiar smell of yeast protein |
| CN110813075A (en) * | 2019-08-27 | 2020-02-21 | 上海普乐拜格健康科技有限公司 | Biological agent for eliminating peculiar smell of wet garbage and preparation method thereof |
| CN111111423A (en) * | 2019-12-30 | 2020-05-08 | 井冈山市傲新华富育种有限公司 | Odor treatment spray for farm, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109010166A (en) * | 2018-07-05 | 2018-12-18 | 上海普缇康生物技术有限公司 | A kind of plant source small molecule cleaning agent for mouth cavity and preparation method thereof |
| CN109293732A (en) * | 2018-10-23 | 2019-02-01 | 宣城市福贝宠物食品有限公司 | Method for removing peculiar smell of yeast protein |
| CN110813075A (en) * | 2019-08-27 | 2020-02-21 | 上海普乐拜格健康科技有限公司 | Biological agent for eliminating peculiar smell of wet garbage and preparation method thereof |
| CN111111423A (en) * | 2019-12-30 | 2020-05-08 | 井冈山市傲新华富育种有限公司 | Odor treatment spray for farm, and preparation method and application thereof |
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| CN103357056B (en) | 2016-03-23 |
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