CN102580132A - Household harmful microorganism elimination and deodorization solution and preparation method thereof - Google Patents
Household harmful microorganism elimination and deodorization solution and preparation method thereof Download PDFInfo
- Publication number
- CN102580132A CN102580132A CN2012100213070A CN201210021307A CN102580132A CN 102580132 A CN102580132 A CN 102580132A CN 2012100213070 A CN2012100213070 A CN 2012100213070A CN 201210021307 A CN201210021307 A CN 201210021307A CN 102580132 A CN102580132 A CN 102580132A
- Authority
- CN
- China
- Prior art keywords
- parts
- harmful
- household
- inoculated
- disappears
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses a household harmful microorganism elimination and deodorization solution and a preparation method thereof. The household harmful microorganism elimination and deodorization solution comprises the following components in part: 13 to 21 parts of photosynthetic bacteria, 11 to 16 parts of bacillus, 23 to 31 parts of lactic acid bacteria, 17 to 24 parts of yeast, 11 to 16 parts of nitrifying bacteria and 10 to 15 parts of actinomycetes. Meanwhile, the invention also discloses the preparation method of the household harmful microorganism elimination and deodorization solution. According to the household harmful microorganism elimination and deodorization solution disclosed by the invention, various group bacteria which are beneficial to win-win cooperation of floras and have synergism are formed by self expanding culture and mixed expanding culture; by the expanding culture acid production, the pH is reduced to 3.6 to 4.8; and various harmful microorganism elimination and deodorization extension derivatives are also generated. Therefore, various harmful microorganisms in the environments of living, working and office places of people, restaurants for business activities, hotels, stations, public toilets, household garbage cans (pools) and the like can be inhibited so as to be eliminated and the generation problem of odor is solved from the source of generating obnoxious gas (hydrogen sulfide, ammonia and the like). The household harmful microorganism elimination and deodorization solution has high harmful microorganism elimination and deodorization efficiency, high speed and strong adaptability. Meanwhile, toxic and harmful substances in the environments can be decomposed and converted into nontoxic and harmless substances.
Description
Technical field
The invention belongs to the small stores field, be specifically related to a kind of household harmful deodorant liquid and preparation method thereof that disappears.
Background technology
Along with improving constantly of economic development, social progress, the enhancing of people's environmental health consciousness and quality of life; Contacts are frequent, and the harmful deodorization problem that disappears of the restaurant of life, work, office space, commercial activity, hotel, station, public lavatory, house refuse bucket (pond) etc. is more and more paid attention to.Harmful microorganism mainly is meant harmful saprophytic microbe and harmful source of disease microorganism, common harmful saprophytic microbe: like Bacillus proteus, penicillium sp, aspergillus, Rhizopus, Aspergillus flavus, false monospore bacillus; Harmful source of disease microorganism and virus: like Yersinia pestis, yellow fever virus (black strain), anthrax brood cell bacterium, bacillus botulinus, Burkholderia mallei, Diplococcus pneumoniae, staphylococcus, streptococcus, Salmonella, shigella etc.; The general designation harmful microorganism.Harmful microorganism is to make to cause stench and the ill source of people, animal.Foul gas mainly is some sulfur-containing compounds and nitrogen-containing compound; Like hydrogen sulfide, methanthiol, thioether, ammonia etc.; Has strong impulse property abnormal flavour; Very harmful to human body can get into human body through different approaches such as respiratory tract, eye, skins, make the people feel bad, dizzy, stay and wherein cause nervous system damage for a long time.Wherein particularly evident with the toxicity of hydrogen sulfide; The people sucked 70-150mg/m3/1-2 hour, respiratory tract and eye irritation occurred, inhaled olfactory fatigue after 2-5 minute; No longer smell foul smell; Sucked 300mg/m3/1 hour, the 6-8 minute acute irritation symptoms that goes out to lose face, Long contact time causes pulmonary edema, bronchitis and pneumonia, headache, giddy, instability of gait, feels sick, vomits slightly; Suck the 1000mg/m3/ several seconds, occur acute poisoning very soon, respiratory paralysis behind the accelerated breathing and death.Therefore; Must develop a kind of efficiently, the household harmful deodorant liquid that disappears fast; Harmful microorganism with to the restaurant of people's life, work, office space, commercial activity, hotel, station, public lavatory, house refuse bucket (pond) etc. suppresses; Solve the propagation and the malodorous generation of source of disease from the source, improve its space and surrounding enviroment quality.
The life of harmful microorganism through air, animals and plants remains, house refuse, people, work activities etc. are bred and are propagated, and (except that the aseptic working environment of manual work) we can say ubiquitously in the human settlement, growing, breeds, propagating constantly; So people, animal cause healthy, be to have suppressed harmful microbe breeding and propagation because beneficial microbe is in the ascendance.One has control environment and approach harmful microorganism will to breed and propagate serious threat environmental health and people's health in a large number.In people's life, work, the environment of an activation, particularly litter basket (pond), kitchen, toilet, public lavatory etc., harmful microorganism can be made and cause pathophoresis and foul odour generation.Along with Social Culture, expanding economy, people's social interactions activity is frequent, urban population density increases, demand raw-food material amount is big and multiformity, Excreta and house refuse increase.Increase the harmful microbe route of transmission, constantly increases suppressing harmful microorganism and environment deodorant pressure.
In the human settlement, suppress both at home and abroad at present to be to use chemicals sterilization and deodorization mostly, but to use the chemical products mask malodors in harmful microorganism and the deodorization, but can not change the generation of stink or stop it to distribute.Chemicals virus killing, deodorization have side effects to people's health, also cause second environmental pollution simultaneously.Also have microbial deodorant in recent years,,, do not obtain excellent popularization and use suppressing remarkable inadequately and quick on harmful bacterium and the deodorization effect owing to the deficiency on screening, optimization, assembly, symbiosis and the production technology of probiotics.So it is low to research and develop out a kind of cost, efficient is high, and speed is fast, the household of the wide adaptability harmful deodorant liquid and preparation method thereof that disappears.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of household harmful deodorant liquid that disappears.Disappear harmful odor removal efficient of these article is high, and speed is fast, and wide adaptability, has no side effect at safety non-toxic, and is harmless to people, animal, non-secondary pollution.Another object of the present invention provides the disappear method for preparing of harmful deodorant liquid of above-mentioned household.
Technical scheme
In order to reach goal of the invention, the present invention specifically realizes like this: a kind of household harmful deodorant liquid that disappears is characterized in that comprising the component of following parts by weight:
11～16 parts of 13～21 parts of bacillus cereuss of photosynthetic bacteria
17～24 parts in 23～31 parts of yeast of lactobacillus
10～15 parts in 11～16 parts of actinomycetes of nitrobacteria
Viable count>=1 * 10 in the said bacterium liquid
8Cfu/ml.
Said photosynthetic bacteria is that Chlorobium and/or bacillus rubidus belong to and/or purple nonsulfur bacteria belongs to.
Bacillus cereus is bacillus subtilis and/or Bacillus cereus and/or Bacillus licheniformis.
Said lactobacillus is Lactobacillus and/or moral formula Lactobacillus and/or Bifidobacterium.
Said yeast is torulopsis and/or Hansenula yeast and/or beer yeast and/or candida mycoderma.
Said nitrobacteria is Nitromonas and/or Nitrosomas.
Said actinomycetes are Nocardia and/or pink mold cyst Pseudomonas and/or actinoplanes and/or streptomyces.
The disappear method for preparing of harmful deodorant liquid of a kind of household is characterized in that may further comprise the steps:
(1) activation expanding propagation:
Photosynthetic bacteria through slant activation, is inoculated in the fluid medium, cultivates 48～72h under 25～35 ℃ of conditions;
Bacillus cereus through slant activation, is inoculated in the fluid medium, cultivates 48～72h under 25～35 ℃ of conditions.
Lactobacillus through slant activation, is inoculated in the fluid medium, cultivates 48～72h under 25～35 ℃ of conditions.
Yeast through slant activation, is inoculated in the fluid medium, cultivates 48～72h under 25～35 ℃ of conditions.
Nitrobacteria through slant activation, is inoculated in the fluid medium, cultivates 72～96h under 25～35 ℃ of conditions.
Actinomycetes through slant activation, are inoculated in the fluid medium, cultivate 48～72h under 25～35 ℃ of conditions.
(2) counting mixes:
Connect this step (1) and calculate viable bacteria; Detect photosynthetic bacteria, bacillus cereus, lactobacillus, yeast, nitrobacteria, actinomycetic bacterium number, bacterium liquid is mixed obtaining mixed bacteria liquid in 13～21 parts of photosynthetic bacterias, 11～16 parts of bacillus cereuss, 23～31 parts of lactobacilluss, 17～24 parts of yeast, 11～16 parts of nitrobacterias, 10～15 parts of actinomycetic component ratios.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 9～11 parts of molasses, 90～100 parts aquesterilisa is cultivated 96～120h at 25-35 ℃ of lower seal, and harmful deodorizing stock solution obtains disappearing.
(4) the secondary symbiosis spreads cultivation:
Stock solution is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 25-35 ℃ of lower seal, obtain the household harmful deodorant liquid secondary liquid that spreads cultivation that disappears.
(5) three symbiosis spread cultivation:
The secondary symbiosis liquid that spreads cultivation is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 24-36 ℃ of lower seal, obtain the household harmful deodorant liquid finished product that disappears.
Beneficial effect: the present invention compares with method with traditional product, has following advantage:
(1) consumption is little, and consumption can be on a declining curve along with the growth of service time, so cost is low.
(2) nontoxic, harmless, no burn into have no irritating odor, noresidue, can not produce secondary pollution.
(3) suppress, eliminate harmful microorganism, decompose, be converted into nontoxic, harmless, odorless material, the source of generation of fundamentally removing to eliminate stink to poisonous, harmful, product sordes matter.
(4) efficient, quick, can in very short time, suppress smelly source rapidly, no longer produce foul smell, make surrounding air pure and fresh.
(5) in people's household, work, office space, the restaurant of commercial activity, hotel, station, public lavatory, house refuse bucket (pond) harmful microorganism grows and use in the place of producing smelly source, the harmful deodorizing effect that disappears is remarkable, wide adaptability.
The specific embodiment
Embodiment 1:
(1) activation expanding propagation:
The cultivation of A photosynthetic bacteria: the former bacterium of green sulphur bacteria is inoculated in the liquid test tube with cover, 30 ℃ with illumination cultivation 3～5 days, obtain bacteria suspension; Get 1% bacteria suspension and put in the triangular flask, its culture medium is improvement normal form culture medium (through 121 ℃ of sterilizations in 30 minutes), and the secondary that then vibrates is cultivated, 30 ℃ of temperature, and 120r/min cultivates the secondary culture that obtains bacillus rubidus behind 48～72h.
The cultivation of B bacillus cereus: the former strain of bacillus subtilis is placed on the nutrient agar; Do primary inclined plane for 36 ℃ and cultivate 24h, be inoculated into the secondary that vibrates in the nutrient broth medium then and cultivate 36 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of bacillus cereus.
The cultivation of C lactobacillus: the former strain of lactobacillus is placed on the Carnis Bovis seu Bubali cream culture medium; Do primary inclined plane for 36 ℃ and cultivate 24h, be inoculated into the secondary that vibrates in the same liquid malt extract medium then and cultivate 28 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of lactobacillus.
The saccharomycetic cultivation of D: place lactic acid-Rhizoma Solani tuber osi-dextrose culture-medium to say the former strain of torulopsis; Doing primary inclined plane for 28 ℃ cultivates; Being inoculated into the secondary that vibrates in the fluid medium of same component then cultivates; 28 ℃ of temperature, 120r/min gets saccharomycetic secondary culture behind cultivation 48～72h;
The cultivation of E nitrobacteria: nitrobacteria comprises Nitromonas and Nitrosomas; Respectively the ammonia nitrogen in the water is converted into nitrite and nitrate; At 25 ℃ of slant culture, be inoculated into then and carry out 2 grades of shaken cultivation in the triangular flask, 25 ℃ of temperature; 120r/min, cultivate behind 72～96h the secondary culture of nitrobacteria; Described Nitrosomas culture medium: ammonium sulfate 0.5g, ammonium chloride 0.3g, green vitriol 0.03g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.03g, calcium chloride 0.05g, distilled water 1000ml, pH value 7～7.2;
Described nitrifier culture medium: sodium nitrite 1g, magnesium sulfate 0.03g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.7g, potassium dihydrogen phosphate 0.24g, sodium carbonate 1g, distilled water 1000ml, pH value 7～7.2;
The actinomycetic cultivation of F: the former bacterium of Nocard's bacillus places on the improvement Gause I culture medium, does primary inclined plane for 28 ℃ and cultivates, and be inoculated into then and make shaken cultivation in the triangular flask, 28 ℃ of temperature, 120r/min gets nocardial secondary culture behind cultivation 48～72h;
(2) counting mixes
Count plate; Detect green sulphur bacteria, bacillus subtilis, lactobacillus, torulopsis, Nitromonas, anti-Nitromonas and Nocard's bacillus number; By following component: 15 parts green sulphur bacteria, 13 parts of bacillus subtilises, 25 parts lactobacillus delbrueckii, 20 parts torulopsis, 7 parts nitrifier, 7 parts denitrifying bacterium, 13 parts Nocard's bacillus, mix mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 9～11 parts of molasses, 90～100 parts aquesterilisa is cultivated 96～120h at 25-35 ℃ of lower seal, and harmful deodorizing stock solution obtains disappearing;
(4) the secondary symbiosis spreads cultivation:
Stock solution is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 25-35 ℃ of lower seal, obtain the household harmful deodorant liquid secondary liquid that spreads cultivation that disappears.
(5) three symbiosis spread cultivation:
The secondary symbiosis liquid that spreads cultivation is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 24-36 ℃ of lower seal, obtain the household harmful deodorant liquid finished product that disappears.
Embodiment 2:
(1) activation expanding propagation
The cultivation of A photosynthetic bacteria: the former bacterium of bacillus rubidus is inoculated in the liquid test tube with cover, cultivated 3～5 days down with illumination for 31 ℃, obtain bacteria suspension; Get 1% bacteria suspension and put in the triangular flask, its culture medium is Improvement type culture medium (through 121 ℃ of sterilizations in 30 minutes), and the secondary that then vibrates is cultivated, 31 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of bacillus rubidus.
The B bacillus cereus is cultivated: the former strain of Bacillus cereus places on the beef-protein medium; Doing primary inclined plane for 32 ℃ cultivates; Being inoculated into the secondary that vibrates in the same liquid Semen Maydis pulp culture medium then cultivates; 32 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of Bacillus cereus.
The cultivation of C lactobacillus: the former strain of moral formula lactobacillus is placed on the malt extract medium; Doing primary inclined plane for 32 ℃ cultivates; Being inoculated into the secondary that vibrates in the same liquid malt extract medium then cultivates; 32 ℃ of temperature, 120r/min, the secondary culture of De Deshi lactobacillus behind cultivation 48～72h.
The saccharomycetic cultivation of C: the former strain of Hansenula yeast is placed lactic acid--on Rhizoma Solani tuber osi-dextrose culture-medium; Doing primary inclined plane for 31 ℃ cultivates; Being inoculated into the secondary that vibrates in the fluid medium of same component then cultivates; 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains Hansenula yeast behind 48～72h.
The cultivation of D nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier.Respectively the ammonia nitrogen in the water is converted into nitrite and nitrate,, is inoculated into then and carries out 2 grades of shaken cultivation in the triangular flask at 31 ℃ of slant culture, 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains nitrobacteria behind 72～96h.
Described nitrococcus culture medium: sodium chloride 0.3g, magnesium sulfate 0.03g, ammonium sulfate 0.5g, calcium chloride 0.05g, green vitriol 0.03g, dipotassium hydrogen phosphate 1g, distilled water 1000ml, pH value 7～7.2.
Described nitrifier culture medium: sodium nitrite 1g, magnesium sulfate 0.03g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.75g, potassium dihydrogen phosphate 0.25g, sodium carbonate 1g, distilled water 1000ml, pH value 7～7.2.
The cultivation of E defence line bacterium: the former bacterium of pink mold cyst bacterium is placed on the improvement Gause I culture medium, do primary inclined plane for 31 ℃ and cultivate, be inoculated into then and make shaken cultivation in the triangular flask, 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains the pink mold cyst bacterium behind 48～72h.
(2) counting mixes
Count plate; Detect bacillus rubidus, Bacillus cereus, moral formula lactobacillus, Hansenula yeast, nitrifier, denitrifying bacterium and pink mold cyst bacterium number; By following component: 15 parts bacillus rubidus, 13 parts of Bacillus cereus, 25 parts moral formula lactobacillus, 20 parts Hansenula yeast, 7 parts nitrifier, 7 parts denitrifying bacterium, 13 parts pink mold cyst bacterium mix, mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 9～11 parts of molasses, 90～100 parts aquesterilisa is cultivated 96～120h at 25-35 ℃ of lower seal, and harmful deodorant liquid stock solution obtains disappearing.
(4) the secondary symbiosis spreads cultivation:
Stock solution is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 25-35 ℃ of lower seal, obtain the household harmful deodorant liquid secondary liquid that spreads cultivation that disappears
(5) three symbiosis spread cultivation:
The secondary symbiosis liquid that spreads cultivation is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 24-36 ℃ of lower seal, obtain the household harmful deodorant liquid finished product that disappears.
Embodiment 3
(1) activation expanding propagation
The cultivation of A photosynthetic bacteria: the former bacterium of purple nonsulfur bacteria is inoculated in the liquid test tube with cover, and 31 ℃ of illumination cultivation 3～5 days obtain bacteria suspension; Get 1% bacteria suspension and put in the triangular flask, its culture medium is improvement normal form culture medium (through 121 ℃ of sterilizations in 30 minutes), and the secondary that then vibrates is cultivated, 31 ℃ of temperature, and 120r/min cultivates the secondary culture that obtains purple nonsulfur bacteria behind 48～72h.
The cultivation of B bacillus cereus: the former strain of Bacillus licheniformis is placed on the malt extract medium; Doing primary inclined plane for 31 ℃ cultivates; Being inoculated into the secondary that vibrates in the same liquid malt extract medium then cultivates; 31 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of Bacillus licheniformis.
The cultivation of C lactobacillus: the former strain of bacillus bifidus is placed on the malt extract medium; Do primary inclined plane for 31 ℃ and cultivate, be inoculated into the secondary that vibrates in the same liquid malt extract medium then and cultivate 31 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of bacillus bifidus.
The saccharomycetic cultivation of D: the former strain of candida mycoderma is placed lactic acid--on Rhizoma Solani tuber osi-dextrose culture-medium; Doing primary inclined plane for 31 ℃ cultivates; Being inoculated into the secondary that vibrates in the fluid medium of same component then cultivates; 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains candida mycoderma behind 48～72h.
The cultivation of E nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier.Respectively the ammonia nitrogen in the water is converted into nitrite and nitrate,, is inoculated into then and carries out 2 grades of shaken cultivation in the triangular flask at 25 ℃ of slant culture, 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains nitrobacteria behind 48～72h.
Described Nitrosomas culture medium: ammonium sulfate 0.5g, ammonium chloride 0.3g, green vitriol 0.03g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.03g, calcium chloride 0.05g, distilled water 1000ml, pH value 7～7.2.
Described nitrifier culture medium: sodium nitrite 1g, magnesium sulfate 0.03g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.7g, potassium dihydrogen phosphate 0.24g, sodium carbonate 1g, distilled water 1000ml, pH value 7～7.2.
The actinomycetic cultivation of F: the former bacterium of actinoplanes is placed on the improvement Gause I culture medium; Do primary inclined plane for 31 ℃ and cultivate, be inoculated into then and make shaken cultivation in the triangular flask, 31 ℃ of temperature; 120r/min cultivates the secondary culture that obtains actinoplanes behind 48～72h.
(2) counting mixes
Count plate; Purple nonsulfur bacteria, Bacillus licheniformis, bacillus bifidus, candida mycoderma, nitrifier, denitrifying bacterium and actinoplanes number; By following component: 15 parts of bacillus rubiduses, 13 Bacillus licheniformis, 26 parts of bacillus bifiduss, 21 parts of candida mycodermas, 6 parts of nitrifiers, 6 parts of denitrifying bacteriums and 13 parts of actinoplanes mix, mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 9～11 parts of molasses, 90～100 parts aquesterilisa is cultivated 96～120h at 25-35 ℃ of lower seal, and harmful deodorizing stock solution obtains disappearing.
(4) the secondary symbiosis spreads cultivation:
Stock solution is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 25-35 ℃ of lower seal, obtain the household harmful deodorant liquid secondary liquid that spreads cultivation that disappears.
(5) three symbiosis spread cultivation:
The secondary symbiosis liquid that spreads cultivation is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 24-36 ℃ of lower seal, obtain the household harmful deodorant liquid finished product that disappears.
Claims (9)
1. a household harmful deodorant liquid that disappears is characterized in that comprising the component of following parts by weight:
11～16 parts of 13～21 parts of bacillus cereuss of photosynthetic bacteria
17～24 parts in 23～31 parts of yeast of lactobacillus
10～15 parts in 11～16 parts of actinomycetes of nitrobacteria.
2. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that viable count>=1 * 10 in the said probiotic bacteria liquid
8Cfu/ml.
3. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that said photosynthetic bacteria is that bacillus rubidus genus and/or Chlorobium and/or purple nonsulfur bacteria belong to.
4. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that said bacillus cereus is bacillus subtilis and/or Bacillus licheniformis and/or Bacillus cereus.
5. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that said lactobacillus is Lactobacillus and/or Bifidobacterium and/or moral formula Lactobacillus.
6. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that said yeast is Hansenula yeast and/or torulopsis and/or beer yeast and/or candida mycoderma.
7. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that said nitrobacteria is nitric acid Pseudomonas and/or nitrous acid Pseudomonas mixed vaccine.
8. a kind of household according to claim 1 harmful deodorant liquid that disappears is characterized in that said actinomycetes are pink mold cyst Pseudomonas and/or streptomyces and/or Nocardia and/or actinoplanes.
9. the household method for preparing of harmful deodorant liquid that disappears is characterized in that may further comprise the steps:
(1) activation expanding propagation:
Photosynthetic bacteria through slant activation, is inoculated in the fluid medium, cultivates 36～72h under 25～35 ℃ of conditions;
Bacillus cereus through slant activation, is inoculated in the fluid medium, cultivates 36～72h under 25～35 ℃ of conditions;
Lactobacillus through slant activation, is inoculated in the fluid medium, cultivates 36～72h under 25～35 ℃ of conditions;
Yeast through slant activation, is inoculated in the fluid medium, cultivates 36～72h under 25～35 ℃ of conditions;
Nitrobacteria through slant activation, is inoculated in the fluid medium, cultivates 36～72h under 25～35 ℃ of conditions;
Actinomycetes through slant activation, are inoculated in the fluid medium, cultivate 36～72h under 25～35 ℃ of conditions;
(2) counting mixes:
Connect this step (1) and calculate viable bacteria; Detect photosynthetic bacteria, bacillus cereus, lactobacillus, yeast, nitrobacteria, actinomycetic bacterium number; Press following component: 13～21 parts of photosynthetic bacterias, 11～16 parts of bacillus cereuss, 23～31 parts of lactobacilluss, 17～24 parts of yeast, 11～16 parts of nitrobacterias, 10～15 parts of actinomycetes mix, and obtain mixed bacteria liquid;
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 9～11 parts of molasses, 90～100 parts aquesterilisa is cultivated 96～120h at 25-35 ℃ of lower seal, and harmful deodorizing stock solution obtains disappearing;
(4) the secondary symbiosis spreads cultivation:
Stock solution is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 25-35 ℃ of lower seal, obtain the household harmful deodorant liquid secondary liquid that spreads cultivation that disappears;
(5) three symbiosis spread cultivation:
The secondary symbiosis liquid that spreads cultivation is inoculated in molasses culture medium, 1～2 of umber part of stock solution by volume, 2～3 parts of molasses, 30～35 parts of aquesterilisa are cultivated 48～72h at 24-36 ℃ of lower seal, obtain the household harmful deodorant liquid finished product that disappears.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100213070A CN102580132A (en) | 2012-01-31 | 2012-01-31 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100213070A CN102580132A (en) | 2012-01-31 | 2012-01-31 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102580132A true CN102580132A (en) | 2012-07-18 |
Family
ID=46469722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100213070A Pending CN102580132A (en) | 2012-01-31 | 2012-01-31 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102580132A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103357056A (en) * | 2013-07-22 | 2013-10-23 | 上海绿博生物科技发展有限公司 | Healthy biological spraying agent for removing peculiar smell and preparation method thereof |
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
| CN104371959A (en) * | 2014-11-18 | 2015-02-25 | 吴山功 | Biological deodorizing and fly-destroying complex microbial preparation and application thereof |
| CN104399098A (en) * | 2014-11-06 | 2015-03-11 | 临沂清宇环境资源工程设备有限公司 | Composite microbial deodorant and preparation method thereof |
| CN104840987A (en) * | 2014-11-19 | 2015-08-19 | 合肥宏士达环保科技有限责任公司 | Composite microbial deodorant and preparation method thereof |
| CN105126143A (en) * | 2015-07-27 | 2015-12-09 | 宁波江东仑斯福环保科技有限公司 | Organic waste deodorizer and preparation method thereof |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
| CN105969698A (en) * | 2016-07-13 | 2016-09-28 | 卿龙集团有限公司 | Composite microbial inoculant for water purification |
| CN106310320A (en) * | 2015-06-15 | 2017-01-11 | 仁维国际股份有限公司 | Deodorant and application thereof |
| CN107297136A (en) * | 2017-08-09 | 2017-10-27 | 深圳市清绿源环境技术股份有限公司 | A kind of complex microorganism garbage deodorant and preparation method thereof |
| CN108211692A (en) * | 2017-12-29 | 2018-06-29 | 广州广牧丰生物技术有限公司 | A kind of biological deodorant and its application |
| CN108251332A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN112791572A (en) * | 2021-03-10 | 2021-05-14 | 天水师范学院 | Ammonia removal agent for chicken farm and preparation method thereof |
| CN112795498A (en) * | 2019-11-14 | 2021-05-14 | 东莞市蓝绅环保有限公司 | Deodorant for deodorizing and purifying water and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544959A (en) * | 2009-05-05 | 2009-09-30 | 薛松晓 | Complex probiotics for environmental remediation, preparation method and application thereof |
| CN102228705A (en) * | 2011-06-15 | 2011-11-02 | 江苏新琦环保有限公司 | Biological deodourant and preparation method thereof |
-
2012
- 2012-01-31 CN CN2012100213070A patent/CN102580132A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544959A (en) * | 2009-05-05 | 2009-09-30 | 薛松晓 | Complex probiotics for environmental remediation, preparation method and application thereof |
| CN102228705A (en) * | 2011-06-15 | 2011-11-02 | 江苏新琦环保有限公司 | Biological deodourant and preparation method thereof |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103357056B (en) * | 2013-07-22 | 2016-03-23 | 上海绿博生物科技发展有限公司 | A kind of biological spray of health type removing abnormal flavour and preparation method thereof |
| CN103357056A (en) * | 2013-07-22 | 2013-10-23 | 上海绿博生物科技发展有限公司 | Healthy biological spraying agent for removing peculiar smell and preparation method thereof |
| CN103468608A (en) * | 2013-08-27 | 2013-12-25 | 重庆瑞宝环保工程有限公司 | Complex flora for sewage treatment and culture method of complex flora |
| CN104399098A (en) * | 2014-11-06 | 2015-03-11 | 临沂清宇环境资源工程设备有限公司 | Composite microbial deodorant and preparation method thereof |
| CN104371959A (en) * | 2014-11-18 | 2015-02-25 | 吴山功 | Biological deodorizing and fly-destroying complex microbial preparation and application thereof |
| CN104371959B (en) * | 2014-11-18 | 2017-12-05 | 吴山功 | Biological deodorizing fly eradication composite bacteria agent and its application |
| CN104840987A (en) * | 2014-11-19 | 2015-08-19 | 合肥宏士达环保科技有限责任公司 | Composite microbial deodorant and preparation method thereof |
| CN106310320A (en) * | 2015-06-15 | 2017-01-11 | 仁维国际股份有限公司 | Deodorant and application thereof |
| CN105126143B (en) * | 2015-07-27 | 2017-11-10 | 宁波高新区斯汀环保科技有限公司 | A kind of organic waste deodorant and preparation method thereof |
| CN105126143A (en) * | 2015-07-27 | 2015-12-09 | 宁波江东仑斯福环保科技有限公司 | Organic waste deodorizer and preparation method thereof |
| CN105779335A (en) * | 2016-03-15 | 2016-07-20 | 甘肃省农业科学院生物技术研究所 | Formula and preparation method of micro-ecological deodorant |
| CN105969698A (en) * | 2016-07-13 | 2016-09-28 | 卿龙集团有限公司 | Composite microbial inoculant for water purification |
| CN107297136A (en) * | 2017-08-09 | 2017-10-27 | 深圳市清绿源环境技术股份有限公司 | A kind of complex microorganism garbage deodorant and preparation method thereof |
| CN108211692A (en) * | 2017-12-29 | 2018-06-29 | 广州广牧丰生物技术有限公司 | A kind of biological deodorant and its application |
| CN108251332A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN112795498A (en) * | 2019-11-14 | 2021-05-14 | 东莞市蓝绅环保有限公司 | Deodorant for deodorizing and purifying water and preparation method thereof |
| CN112791572A (en) * | 2021-03-10 | 2021-05-14 | 天水师范学院 | Ammonia removal agent for chicken farm and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102580132A (en) | Household harmful microorganism elimination and deodorization solution and preparation method thereof | |
| CN102578156A (en) | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof | |
| CN103031253B (en) | Compound microorganism living bacteria agent, method for preparing same and applications | |
| CN102228705B (en) | Preparation method of biological deodourant | |
| CN103992968B (en) | Preparation method of household garbage deodorizing and sterilizing composite inoculant | |
| CN102851231B (en) | Functional strain, and microbial agent and application thereof | |
| CN101358171B (en) | Complex bacteria for preprocessing of restaurant garbage, preparation method and application thereof | |
| CN106731775A (en) | A kind of expansion type compound biological enzyme deodorant and preparation method and application | |
| CN106282038A (en) | A kind of composite efficient microbial deodorant and preparation method thereof | |
| JP2810308B2 (en) | Deodorant containing microorganisms and their cells | |
| CN104399098A (en) | Composite microbial deodorant and preparation method thereof | |
| CN104307012A (en) | Deodorant special for livestock and poultry farms and application thereof | |
| CN101269227A (en) | Biological agent for purifying air and preparation method thereof | |
| CN109609401A (en) | A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof | |
| CN102258796A (en) | Microbial deodorant and preparation method thereof | |
| CN102813948A (en) | Preparation method of composite biological deodorant | |
| CN106967643A (en) | A kind of Novel compound microbial agent and preparation method thereof | |
| CN102813949A (en) | Microbial environment protectant | |
| CN104840987A (en) | Composite microbial deodorant and preparation method thereof | |
| CN103468619A (en) | Microbial preparation for purifying air | |
| CN102872475A (en) | Biological agent for deodorizing and reducing chromium and preparation method thereof | |
| CN109092049A (en) | A kind of biologic ferment deodorant | |
| CN105255761B (en) | A kind of deodorization microbial inoculum and its application method for organic waste treatment | |
| CN107570002A (en) | Composite type deodorization agent for toilet deodorization and preparation method thereof | |
| CN108587999A (en) | A kind of screening and breeding method for lavatory and refuse depot deodorization bacterial strain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120718 |