CN102578156A - Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof - Google Patents
Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof Download PDFInfo
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Abstract
The invention discloses domestic garbage harmful microorganism elimination deodorization liquid and a preparation method thereof. The domestic garbage harmful microorganism elimination deodorization liquid comprises probiotics components as follows: photosynthetic bacteria accounting for 11 to 17 parts, bacillus accounting for 9 to 13 parts, lactic acid bacteria accounting for 24 to 31 parts, saccharomycete accounting for 17 to 23 parts, nitrifiers accounting for 8 to 12 parts, acidithiobacillus accounting for 8 to 12 parts and ray fungi accounting for 10 to 15 parts. Meanwhile, the invention further discloses the preparation method of the domestic garbage harmful microorganism elimination deodorization liquid. The domestic garbage harmful microorganism elimination deodorization liquid performs self symbiotic propagation to generate group bacteria containing a plurality of beneficial bacteria that can achieve co-existence, co-prosperity and synergism; propagation can produce acid, so that the pH value is decreased to reach 3.6 to 4.8; and various beneficial derivatives for harmful microorganism elimination deodorization are generated. Various harmful microorganisms in domestic garbage can be inhibited so as to be eliminated, so that the generation of malodorous gas can be solved from the source of malodorous gas (hydrogen sulfide, ammonia gas and the like). The harmful microorganism elimination deodorization effect is good, the speed is high, and the suitability is strong. Meanwhile, toxic and harmful substances are converted to nontoxic and harmless substances facilitating growth and absorption of plants during the decomposing process of domestic garbage.
Description
Technical field
The invention belongs to field of environment protection, be specifically related to a kind of house refuse harmful deodorant liquid and preparation method thereof that disappears.
Background technology
Along with the improving constantly of society, economic development, people's quality of life, assanation consciousness strengthen, the harmful deodorizing problem that disappears of urban and rural domestic refuse is more and more paid attention to.Harmful microorganism mainly is meant harmful saprophytic microbe and harmful pathogenic microorganism, and common harmful saprophytic microbe is like proteus, mould, aspergillus, rhizopus, aspergillus flavus, false monospore bacillus.Harmful pathogeny microorganism and virus as: Yersinia pestis, yellow fever virus (black strain), anthrax brood cell bacterium, clostridium botulinum, pseudomonas mallei, Diplococcus pneumopniae, staphylococcus, streptococcus, salmonella, Shigella etc.; The general designation harmful microorganism.Harmful microorganism is to cause the ill source that produces with stench of people, animal.Foul gas mainly is some sulfur-containing compounds and nitrogen-containing compound; Like hydrogen sulphide, methyl mercaptan, thioether, ammonia etc.; Has strong impulse property peculiar smell; Very harmful to human body can get into human body through different approaches such as respiratory tract, eye, skins, make the people feel bad, dizzy, stay and wherein cause nervous system damage for a long time.Wherein particularly evident with the toxicity of hydrogen sulphide, the people sucks 70-150mg/m
3Respiratory tract and eye irritation appear in/1-2h, inhale olfactory fatigue after 2-5 minute, no longer smell foul smell, suck 300mg/m
3/ 1h, the 6-8min acute irritation symptoms that goes out to lose face, slightly Long contact time cause pulmonary edema, bronchitis and pneumonia, headache, giddy, instability of gait, feel sick, vomiting; Suck 1000mg/m
3Acute poisoning appears in/the several seconds very soon, respiratory paralysis behind the accelerated breathing and death.Therefore; Must develop a kind of efficient, fast, the house refuse of the wide adaptability harmful deodorant liquid that disappears; Harmful microorganism with to people's house refuse suppresses, and solves the propagation of pathogeny and the generation of stench from the source, improves people's life, production space and surrounding enviroment quality thereof.
The life of harmful microorganism through air, animals and plants remains, house refuse, people, work activities etc. are bred and are propagated, and (except that the aseptic working environment of manual work) we can say ubiquitously in the human settlement, growing, breeds, propagating constantly; So people, animal cause healthy, be to have suppressed harmful microbe breeding and propagation because beneficial microbe is in the ascendance.If adapt circumstance and approach are arranged, harmful microorganism will breed and propagate in a large number, serious threat assanation and people's health.Wherein house refuse is harmful microbe collection seedbed.In house refuse basket (pond), refuse collection, transhipment and garbage disposal plant etc., harmful microorganism can be made and cause transmission and foul odour generation.Along with Social Culture, expanding economy, urban population density increases, demand raw-food material amount is big and diversity, excreta and house refuse constantly increase.Increase the harmful microbe route of transmission, constantly increases with the generation pressure of eliminating stench in house refuse, suppressing harmful microorganism.
In garbage disposal, before rubbish focuses on, be to use chemicals sterilization and deodorizing mostly, but use the chemical products mask malodors at present, but can not change the generation of stink or stop it to distribute, also cause second environmental pollution simultaneously.Rubbish focuses in the field the processing of rubbish, generally adopts garbage loading embeading or incineration treatment of garbage, garbage loading embeading: the land occupation resource also influences surrounding enviroment and water source simultaneously; Waste incineration: the labor intensive and the energy, increase CO2 emission, particulates emission dust is contaminated environment also.This dual mode is not the good mode of the energy-saving and emission-reduction of house refuse.Mode with the microbial deodorant deodorizing also appears in recent years; Because screening, optimization, assembly, symbiosis and fermentation engineering, the technologic deficiency of beneficial bacterium; Suppressing remarkable inadequately and quick on harmful microorganism and the deodorizing effect, do not obtain excellent popularization and use.So; Inhibition is harmful to saprophytic bacteria and harmful pathogenic bacteria to house refuse, useful degraded, the elimination stench of solution house refuse produce from the source; Must research and develop the low cost that can suppress harmful bacterium, eliminate the domestic rubbish disposal of stench generation; High efficiency, wide adaptability, product of energy-saving and emission-reduction simultaneously and preparation method thereof.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of house refuse harmful deodorant liquid that disappears.Disappear harmful odor removal efficient of these article is high, and speed is fast, and wide adaptability, has no side effect at safety non-toxic, and is harmless to people, animal, non-secondary pollution.
Another object of the present invention provides the disappear preparation method of harmful deodorant liquid of above-mentioned house refuse.
Technical scheme: in order to reach goal of the invention, the present invention specifically realizes like this: a kind of house refuse harmful deodorant liquid that disappears comprises the component of following weight portion:
9～13 parts of 11～17 parts of bacillus of photosynthetic bacteria
17～23 parts in 24～31 parts of saccharomycete of lactic acid bacteria
8～12 parts on 8～12 parts of sulphur bars of nitrifying bacteria bacterium
8～12 parts in actinomycetes
Number of live bacteria of probiotics>=5 * 10 in the said harmful deodorant liquid that disappears
8Cfu/ml.
Said photosynthetic bacteria is bacillus rubidus genus and/or Chlorobium and/or purple nonsulfur bacteria genus.
Said bacillus is bacillus subtilis and/or bacillus licheniformis and/or Bacillus cereus.
Wherein, said lactic acid bacteria is moral formula Lactobacillus and/or lactobacillus and/or Bifidobacterium.
Said saccharomycete is Hansenula yeast and/or torulopsis and/or brewer's yeast and/or Candida.
Said nitrifying bacteria is nitrobacteria and/or Nitrosomas.
Said sulphur bar bacterium is Thiobacillus thioxidans and/or Thiobacillus ferrooxidans and/or grate sulfur thiobacillus.
Said actinomycetes are pink mold cyst Pseudomonas and/or actinoplanes and/or streptomyces and/or Nocardia.
The disappear preparation method of harmful deodorant liquid of a kind of house refuse is characterized in that may further comprise the steps:
(1) activation is expanded numerous:
Photosynthetic bacteria through slant activation, is inoculated in the liquid nutrient medium, cultivates 48～72h under 24～36 ℃ of conditions.
Bacillus through slant activation, is inoculated in the liquid nutrient medium, cultivates 48～72h under 24～36 ℃ of conditions.
Lactic acid bacteria through slant activation, is inoculated in the liquid nutrient medium, cultivates 48～72h under 24～36 ℃ of conditions.
Saccharomycete through slant activation, is inoculated in the liquid nutrient medium, cultivates 48～72h under 24～36 ℃ of conditions.
Nitrifying bacteria through slant activation, is inoculated in the liquid nutrient medium, cultivates 48～72h under 24～36 ℃ of conditions.
Sulphur bar bacterium through slant activation, is inoculated in the liquid nutrient medium, cultivates 48～72h under 24～36 ℃ of conditions.
Actinomycetes through slant activation, are inoculated in the liquid nutrient medium, cultivate 48～72h under 24～36 ℃ of conditions.
(2) counting mixes:
Connect this step (1) and calculate viable bacteria, detect photosynthetic bacteria, bacillus, lactic acid bacteria, saccharomycete, nitrifying bacteria, sulphur bar bacterium, actinomycetic bacterium number; Obtain mixed bacteria liquid by 11～17 parts of photosynthetic bacterias, 9～13 parts of bacillus, 24～31 parts of lactic acid bacterias, 17～23 parts of saccharomycete, 8～12 parts of nitrifying bacterias, 8～12 parts of sulphur bar bacteriums, 10～15 parts of actinomycetic components mixing.
(3) symbiosis spreads cultivation:
Connect this step (2) mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 10～13 parts of molasses, 80～90% aqua sterilisa is cultivated 96～120h at 24-36 ℃ of lower seal, obtains the house refuse harmful deodorant liquid finished product stoste that disappears.
Beneficial effect: the present invention compares with method with traditional product, has following advantage:
(1) consumption is little, and consumption can be on a declining curve along with the growth of service time, so cost is low.
(2) nontoxic, harmless, no burn into have no irritating odor, noresidue, can not produce secondary pollution.
(3) suppress, eliminate the harmful microorganism in the house refuse fast, elimination causes gets on to eliminate the generation of stink in ill harmful bacterium source simultaneously from root, get into beneficial bacterium benign decomposition harmless, odorless.
(4) efficient, quick, can in very short time, suppress smelly source rapidly, no longer produce foul smell, make surrounding air pure and fresh.
(5) in house refuse bucket (pond), collection rotation, garbage disposal or vein engineering, use the harmful deodorizing that disappears, benign decomposition effect highly significant.
Embodiment
Embodiment 1:
(1) activation is expanded numerous:
The cultivation of A photosynthetic bacteria: the former bacterium of bacillus rubidus is inoculated in the liquid test tube with cover, 30 ℃ with illumination cultivation 3～5 days, obtain bacteria suspension; Get 1% bacteria suspension and put in the triangular flask, its medium is improvement normal form medium (through 121 ℃ of sterilizations in 30 minutes), and the secondary that then vibrates is cultivated, 30 ℃ of temperature, and 120r/min cultivates the secondary culture that obtains bacillus rubidus behind 48～72h.
The cultivation of B bacillus: the former bacterial classification of bacillus subtilis is placed on the nutrient agar; Do primary inclined plane for 36 ℃ and cultivate 24h, be inoculated into the secondary that vibrates in the nutrient broth medium then and cultivate 36 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of bacillus.
The cultivation of C lactic acid bacteria: the former bacterial classification of lactobacillus delbrueckii is placed on the beef extract medium; Do primary inclined plane for 28 ℃ and cultivate 24h; Being inoculated into the secondary that vibrates in the same liquid malt extract medium then cultivates; 28 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of lactic acid bacteria.
The saccharomycetic cultivation of D: the former bacterial classification of Hansenula yeast is placed on lactic acid-potato-dextrose culture-medium; Doing primary inclined plane for 28 ℃ cultivates; Being inoculated into the secondary that vibrates in the liquid nutrient medium of same component then cultivates; 28 ℃ of temperature, 120r/min gets saccharomycetic secondary culture behind cultivation 48～72h.
The cultivation of E nitrifying bacteria: nitrifying bacteria comprises nitrococcus and nitrifier; Respectively the ammonia nitrogen in the water is converted into nitrite and nitrate; At 25 ℃ of slant culture, be inoculated into then and carry out 2 grades of shaken cultivation in the triangular flask, 25 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of nitrifying bacteria.
Described Nitrosomas medium: ammonium sulfate 0.5g, ammonium chloride 0.3g, green vitriol 0.03g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.03g, calcium chloride 0.05g, distilled water 1000ml, pH value 7～7.2.
Described nitrifier medium: natrium nitrosum 1g, magnesium sulfate 0.03g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.7g, potassium dihydrogen phosphate 0.24g, sodium carbonate 1g, distilled water 1000ml, pH value 7～7.2.
The cultivation of F sulphur bar bacterium: Thiobacillus thioxidans is inoculated into Starky-Na
2S
2O
3On the agar solid culture medium, do primary inclined plane for 30 ℃ and cultivate, be inoculated into then in the Waksman medium, the secondary that vibrates is cultivated, 30 ℃ of temperature, and 200r/min cultivates 48～72h, when treating that bacterium liquid pH value reaches 2.0 left and right sides, promptly gets the secondary culture.
Said Thiobacillus Starky-Na
2S
2O
3Agar solid culture medium: sulfate of ammoniac 0.2g, epsom salt 0.5g, calcium chloride dihydrate 0.126g, elemental sulfur 10g, agar 30g, distilled water 1000ml, pH value 2～2.5.
Said Thiobacillus Waksman medium: sulfate of ammoniac 0.2g, three water dipotassium hydrogen phosphate 3g, epsom salt 0.5g, calcium chloride dihydrate 0.126g, elemental sulfur 10g, agar 30g, distilled water 1000ml, pH value 3～3.5.
The actinomycetic cultivation of G: the former bacterium of pink mold cyst bacterium places on the improvement Gause I medium, does primary inclined plane for 28 ℃ and cultivates, and be inoculated into then and make shaken cultivation in the triangular flask, 28 ℃ of temperature, 120r/min gets nocardial secondary culture behind cultivation 48～72h.
(2) counting mixes:
Count plate; Detect bacillus rubidus, bacillus subtilis, lactobacillus delbrueckii, Hansenula yeast, nitrifier, denitrifying bacterium, Thiobacillus thioxidans and pink mold cyst bacterium bacterium number; Mix by 15 parts bacillus rubiduses, 13 parts of bacillus subtilises, 24 parts lactobacillus delbrueckii, 21 parts Hansenula yeast, 5 parts nitrifier, 5 parts denitrifying bacterium, 9 parts Thiobacillus thioxidans, 8 parts the component of pink mold cyst bacterium, mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 10～13 parts of molasses, 80～90 parts aqua sterilisa is cultivated 96～120h at 24-36 ℃ of lower seal, obtains the house refuse harmful deodorant liquid that disappears.
Embodiment 2:
(1) activation is expanded numerous:
The cultivation of A photosynthetic bacteria: the former bacterium of green sulphur bacteria is inoculated in the liquid test tube with cover, cultivated 3～5 days down with illumination for 31 ℃, obtain bacteria suspension; Get 1% bacteria suspension and put in the triangular flask, its medium is Improvement type medium (through 121 ℃ of sterilizations in 30 minutes), and the secondary that then vibrates is cultivated, 31 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of green sulphur bacteria.
The B bacillus is cultivated: the former bacterial classification of bacillus licheniformis places on the beef-protein medium; Doing primary inclined plane for 32 ℃ cultivates; Being inoculated into the secondary that vibrates in the same liquid corn steep liquor medium then cultivates; 32 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of bacillus licheniformis.
The cultivation of C lactic acid bacteria: the former bacterial classification of lactobacillus is placed on the malt extract medium; Do primary inclined plane for 32 ℃ and cultivate, be inoculated into the secondary that vibrates in the same liquid malt extract medium then and cultivate 32 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of lactobacillus.
The saccharomycetic cultivation of D: the former bacterial classification of torulopsis is placed lactic acid--on potato-dextrose culture-medium; Doing primary inclined plane for 31 ℃ cultivates; Being inoculated into the secondary that vibrates in the liquid nutrient medium of same component then cultivates; 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains torulopsis behind 48～72h.
The cultivation of E nitrifying bacteria: nitrifying bacteria comprises nitrococcus and nitrifier.Respectively the ammonia nitrogen in the water is converted into nitrite and nitrate,, is inoculated into then and carries out 2 grades of shaken cultivation in the triangular flask at 31 ℃ of slant culture, 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains nitrifying bacteria behind 48～72h.
Described nitrococcus medium: sodium chloride 0.3g, magnesium sulfate 0.03g, ammonium sulfate 0.5g, calcium chloride 0.05g, green vitriol 0.03g, dipotassium hydrogen phosphate 1g, distilled water 1000ml, pH value 7～7.2.
Described nitrifier medium: natrium nitrosum 1g, magnesium sulfate 0.03g, ammonium sulfate 0.01g, dipotassium hydrogen phosphate 0.75g, potassium dihydrogen phosphate 0.25g, sodium carbonate 1g, distilled water 1000ml, pH value 7～7.2.
The cultivation of F sulphur bar bacterium: Thiobacillus ferrooxidans is inoculated on the 9K solid culture medium; Doing primary inclined plane for 30 ℃ cultivates; Being inoculated into the secondary that vibrates in the liquid nutrient medium with component then cultivates; 30 ℃ of temperature, 120r/min cultivates the secondary culture that obtains Thiobacillus ferrooxidans behind 48～72h.
Said Thiobacillus Starky-Na
2S
2O
3Agar solid culture medium: sulfate of ammoniac 0.2g, epsom salt 0.5g, calcium chloride dihydrate 0.126g, elemental sulfur 10g, agar 30g, distilled water 1000ml, pH value 2～2.5.
Said Thiobacillus Waksman medium: sulfate of ammoniac 0.2g, three water dipotassium hydrogen phosphate 3g, epsom salt 0.5g, calcium chloride dihydrate 0.126g, elemental sulfur 10g, agar 30g, distilled water 1000ml, pH value 3～3.5.
The actinomycetic cultivation of G: the former bacterium of actinoplanes is placed on the improvement Gause I medium; Do primary inclined plane for 31 ℃ and cultivate, be inoculated into then and make shaken cultivation in the triangular flask, 31 ℃ of temperature; 120r/min cultivates the secondary culture that obtains deep red micromonospora behind 36～72h.
(2) counting mixes:
Count plate; Detect green sulphur bacteria, bacillus licheniformis, lactobacillus, torulopsis, nitrifier, denitrifying bacterium, Thiobacillus ferrooxidans, actinoplanes bacterium number; By 15 parts green sulphur bacterias, 13 parts bacillus licheniformis, 24 parts lactobacillus, 21 parts torulopsis, 5 parts nitrifier, 5 parts denitrifying bacterium, 9 parts Thiobacillus ferrooxidans, 8 parts actinoplanes, get mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 10～13 parts of molasses, 80～90% aqua sterilisa is cultivated 96～120h at 24-36 ℃ of lower seal, obtains the house refuse harmful deodorant liquid finished product stoste that disappears.
Embodiment 3
(1) disorderly expansion alive is numerous
The cultivation of A photosynthetic bacteria: the former bacterium of purple nonsulfur bacteria is inoculated in the liquid test tube with cover 31 ℃ of light
According to cultivating 3～5 days, obtain bacteria suspension; Get 1% bacteria suspension and put in the triangular flask, its medium is improvement normal form medium (through 121 ℃ of sterilizations in 30 minutes), and the secondary that then vibrates is cultivated, 31 ℃ of temperature, and 120r/min cultivates the secondary culture that obtains purple nonsulfur bacteria behind 48～72h.
The B bacillus is cultivated: the former bacterial classification of cured appearance sporeformer places on the beef-protein medium; Doing primary inclined plane for 32 ℃ cultivates; Being inoculated into the secondary that vibrates in the same liquid corn steep liquor medium then cultivates; 32 ℃ of temperature, 120r/min, cultivate behind 48～72h the secondary culture of cured appearance sporeformer.
The cultivation of C lactic acid bacteria: the former bacterial classification of Bifidobacterium is placed on the malt extract medium; Do primary inclined plane for 31 ℃ and cultivate, be inoculated into the secondary that vibrates in the same liquid malt extract medium then and cultivate 31 ℃ of temperature; 120r/min, cultivate behind 48～72h the secondary culture of Bifidobacterium.
The saccharomycetic cultivation of D: the former bacterial classification of Candida is placed lactic acid--on potato-dextrose culture-medium; Doing primary inclined plane for 31 ℃ cultivates; Being inoculated into the secondary that vibrates in the liquid nutrient medium of same component then cultivates; 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains Candida behind 48～72h.
The cultivation of E nitrifying bacteria: nitrifying bacteria comprises nitrococcus and nitrifier.Respectively the ammonia nitrogen in the water is converted into nitrite and nitrate,, is inoculated into then and carries out 2 grades of shaken cultivation in the triangular flask at 25 ℃ of slant culture, 31 ℃ of temperature, 120r/min cultivates the secondary culture that obtains nitrifying bacteria behind 48～72h.
Described Nitrosomas medium: ammonium sulfate 0.5g, ammonium chloride 0.3g, green vitriol 0.03g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.03g, calcium chloride 0.05g, distilled water 1000ml, pH value 7～7.2.
Described nitrifier medium: natrium nitrosum 1g, magnesium sulfate 0.03g, magnesium sulfate 0.01g, dipotassium hydrogen phosphate 0.7g, potassium dihydrogen phosphate 0.24g, sodium carbonate 1g, distilled water 1000ml, pH value 7～7.2.
The cultivation of F sulphur bar bacterium: the former bacterium of grate sulfur thiobacillus is inoculated on the TC10 solid culture medium; Do primary inclined plane for 30 ℃ and cultivate, be inoculated into then and make shaken cultivation in the triangular flask, 30 ℃ of temperature; 120r/min cultivates the secondary culture that obtains grate sulfur thiobacillus behind 48～72h.
Said Thiobacillus Starky-Na
2S
2O
3Agar solid culture medium: sulfate of ammoniac 0.2g, epsom salt 0.5g, calcium chloride dihydrate 0.126g, elemental sulfur 10g, agar 30g, distilled water 1000ml, pH value 2～2.5.
Said Thiobacillus Waksman medium: sulfate of ammoniac 0.2g, three water dipotassium hydrogen phosphate 3g, epsom salt 0.5g, calcium chloride dihydrate 0.126g, elemental sulfur 10g, agar 30g, distilled water 1000ml, pH value 3～3.5.
The cultivation of G defence line bacterium: the former bacterium of Nocard's bacillus is placed on the improvement Gause I medium; Do primary inclined plane for 31 ℃ and cultivate, be inoculated into then and make shaken cultivation in the triangular flask, 31 ℃ of temperature; 120r/min obtains nocardial secondary culture behind cultivation 48～72h.
(2) counting mixes
Count plate; Detect purple nonsulfur bacteria, cured appearance sporeformer, Bifidobacterium, Candida, nitrifier, denitrifying bacterium, grate sulfur thiobacillus and Nocard's bacillus bacterium number; Mix by mass fraction; 15 parts of purple nonsulfur bacterias, 13 parts of cured appearance sporeformer, 25 parts of Bifidobacteriums, 20 parts of Candidas, 5 parts of nitrifiers, 5 parts of denitrifying bacteriums, 9 parts of grate sulfur thiobacillus, 8 parts of Nocard's bacilluss get mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 10～13 parts of molasses, 80～90% aqua sterilisa is cultivated 96～120h at 24-36 ℃ of lower seal, obtains the house refuse harmful deodorizing finished product stoste that disappears.
Claims (10)
1. a house refuse harmful deodorant liquid that disappears is characterized in that comprising the component of following weight portion:
9～13 parts of 11～17 parts of bacillus of photosynthetic bacteria
17～23 parts in 24～31 parts of saccharomycete of lactic acid bacteria
8～12 parts on 8～12 parts of sulphur bars of nitrifying bacteria bacterium
8～12 parts in actinomycetes.
2. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that viable count>=5 * 10 in the said probio liquid
8Cfu/ml.
3. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said photosynthetic bacteria is that bacillus rubidus genus and/or Chlorobium and/or purple nonsulfur bacteria belong to.
4. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said bacillus is bacillus subtilis and/or bacillus licheniformis and/or Bacillus cereus.
5. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said lactic acid bacteria is lactobacillus and/or Bifidobacterium and/or moral formula Lactobacillus.
6. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said saccharomycete is Hansenula yeast and/or torulopsis and/or brewer's yeast and/or Candida.
7. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said nitrifying bacteria is nitric acid Pseudomonas and/or nitrous acid Pseudomonas.
8. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said sulphur bar bacterium is Thiobacillus thioxidans and/or Thiobacillus ferrooxidans and/or grate sulfur thiobacillus.
9. a kind of house refuse according to claim 1 harmful deodorant liquid that disappears is characterized in that said actinomycetes are pink mold cyst Pseudomonas and/or streptomyces and/or Nocardia and/or actinoplanes.
10. the house refuse preparation method of harmful deodorant liquid that disappears is characterized in that may further comprise the steps:
(1) activation is expanded numerous:
Photosynthetic bacteria through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 24～36 ℃ of conditions.
Bacillus through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 24～36 ℃ of conditions.
Lactic acid bacteria through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 24～36 ℃ of conditions.
Saccharomycete through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 24～36 ℃ of conditions.
Nitrifying bacteria through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 24～36 ℃ of conditions.
Sulphur bar bacterium through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 24～36 ℃ of conditions.
Actinomycetes through slant activation, are inoculated in the liquid nutrient medium, cultivate 36～72h under 24～36 ℃ of conditions.
(2) counting mixes:
Connect this step (1) and calculate viable bacteria, detect photosynthetic bacteria, bacillus, lactic acid bacteria, saccharomycete, nitrifying bacteria, sulphur bar bacterium, actinomycetic bacterium number; Mix by 11～17 parts of photosynthetic bacterias, 9～13 parts of bacillus, 24～31 parts of lactic acid bacterias, 17～23 parts of saccharomycete, 8～12 parts of nitrifying bacterias, 8～12 parts of sulphur bar bacteriums, 10～15 parts of actinomycetic components, obtain mixed bacteria liquid.
(3) symbiosis spreads cultivation:
Connect this step (2) mixed bacteria liquid is inoculated in molasses culture medium, 10～13 of umber parts of mixed bacteria liquids by volume, 10～13 parts of molasses, 80～90% aqua sterilisa is cultivated 96～120h at 24-36 ℃ of lower seal, obtains the house refuse harmful deodorant liquid finished product stoste that disappears.
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