CN114920364A - Mixed-strain fermentation microbial deodorant and preparation method thereof - Google Patents
Mixed-strain fermentation microbial deodorant and preparation method thereof Download PDFInfo
- Publication number
- CN114920364A CN114920364A CN202210572085.5A CN202210572085A CN114920364A CN 114920364 A CN114920364 A CN 114920364A CN 202210572085 A CN202210572085 A CN 202210572085A CN 114920364 A CN114920364 A CN 114920364A
- Authority
- CN
- China
- Prior art keywords
- bacteria
- microbial deodorant
- fermentation
- mixed
- fermentation tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses a mixed fermentation microbial deodorant, which is prepared from the following raw materials: probiotics and culture medium, wherein the probiotics comprise saccharomycetes, bacillus subtilis, bacillus licheniformis, photosynthetic bacteria, nitrobacteria, denitrifying bacteria, actinomycetes, sulfurous bacteria, aspergillus niger, aspergillus oryzae and lactic acid bacteria; the mixed fermentation produces tiny protein or peptide (antibacterial peptide) similar to bacteriocin, such as various lactobacillin and bifidus bacterin, which not only has antagonistic action on staphylococcus, clostridium, salmonella and shigella, but also has bacteriolytic action, and the lactic acid bacteria can improve water quality and inhibit vibrio in water body, and can also eliminate nitrite in water body through direct chemical action and indirect denitrification promoting action of microorganism, thereby playing a role in purifying water quality.
Description
Technical Field
The invention belongs to the technical field of microbial fermentation engineering, and particularly relates to a microbial deodorant fermented by mixed bacteria and a preparation method thereof.
Background
In the production operation process of enterprises in the sewage treatment industry, the garbage treatment industry, the livestock breeding industry, the pharmacy, the food, the chemical industry and the like, a large amount of malodorous gas is inevitably generated, and the malodorous gas causes air pollution to the enterprises and the surrounding environment and great harm to people and various organisms in the pollution range. The above-mentioned industries which easily generate malodor have a long history of using deodorizing agents and other deodorizing techniques to remove malodor, ranging from the use of physical deodorizers to chemical deodorizers, to the present biological deodorizer era (plant deodorizers and microbial deodorizers), and microbial deodorizers have their own advantages: the deodorizing effect is immediate, no secondary pollution is generated, and the production and use processes of the microbial deodorant are harmless to people and environment.
The microbial deodorant sold in the market at present has poor specialty and pertinence due to single probiotics, and the deodorization effect is not ideal.
In order to solve the above problems, an object of the present invention is to provide a microbial deodorant.
Disclosure of Invention
The invention aims to provide a mixed fermentation microbial deodorant, which is prepared from the following raw materials: probiotics and culture medium, wherein the probiotics comprise saccharomycetes, bacillus subtilis, bacillus licheniformis, photosynthetic bacteria, nitrobacteria, denitrifying bacteria, actinomycetes, sulfurous bacteria, aspergillus niger, aspergillus oryzae and lactic acid bacteria.
Preferably, the culture medium comprises cane molasses, peptone, maltooligosaccharide, magnesium sulfate, manganese sulfate, citric acid, dipotassium hydrogen phosphate, sodium acetate, beef extract, yeast extract, glycerol, tween 80, sodium chloride, calcium chloride, sodium hydroxide, potassium dihydrogen phosphate and water.
Preferably, the lactobacillus is bifidobacterium, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus.
Preferably, the raw materials are also added with fragrant fruits, such as citrus, apple, pineapple, etc.
A preparation method of mixed fermentation microbial deodorant powder is provided, wherein the preparation method comprises the following steps:
s1: cleaning the fermentation tank and carrying out steam sterilization;
s2: injecting water into the fermentation tank, and simultaneously starting a steam generator to heat the water body in the fermentation tank;
s3: adding cane molasses, peptone, fragrant fruits and malto-oligosaccharide into the fermentation tank heated in the step S2, and simultaneously turning on a stirring device;
s4: then adding one or more of manganese sulfate, citric acid, sodium acetate, magnesium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and sodium hydroxide into the fermentation tank;
s5: when the temperature in the fermentation tank rises to 35 ℃, stopping heating, screening probiotics according to the using direction of the deodorant, firstly feeding aerobic bacteria, then feeding facultative bacteria, and finally feeding anaerobic bacteria, testing the pH value, and adjusting the initial pH value to 6.5-8; the aerobic bacteria are bacillus subtilis, nitrobacteria, actinomycetes, sulfureted bacteria, aspergillus niger and aspergillus oryzae, the facultative bacteria are saccharomycetes, bacillus licheniformis and denitrifying bacteria, and the anaerobic bacteria are photosynthetic bacteria and lactic acid bacteria;
s6: after all probiotics are put in, the stirring can be stopped after the stirring is continuously carried out for 24 hours at a low speed (32r/min) in a closed state;
s7: fermenting for 48-72 hours to obtain a fermented mature microbial deodorant finished product;
s8: and filtering and canning the obtained fermented and mature microbial deodorant finished product.
Preferably, the water used in S1 is groundwater or purified water, and the temperature of the water is heated to 35 ℃.
Preferably, the addition amount of manganese sulfate, citric acid, sodium acetate, magnesium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and NaOH in the S4 is 0.01-0.1% of the total amount, and the ph value of the liquid culture medium is adjusted to 6.5-7.2.
Preferably, in S5, the aerobic bacteria are administered 48 hours later and then the anaerobic bacteria are administered.
Preferably, in S7, the ph value is used to identify whether the fermentation is mature, and the ph value of the fermentation mature is between 3 and 4.
Has the advantages that: the beneficial effects of the invention are: the invention relates to a green environment-friendly deodorant without secondary pollution, which is prepared by mixing a plurality of probiotics for fermentation, wherein the mixed fermentation is also called mixed fermentation, the mixed fermentation can utilize the combination of different metabolic capacities of the plurality of probiotics to complete the complex metabolic action which is difficult to complete by a single probiotic, can replace certain recombinant engineering bacteria to perform complex multiple metabolic reactions, improves the production efficiency, and the plurality of mixed bacteria generate various active substances such as organic acid, special enzyme systems, acid rhzomorph, hydrogen peroxide and the like for inhibiting the growth of putrefying bacteria by fermentation, wherein the hydrogen peroxide inhibits and kills gram-negative bacteria, escherichia coli and salmonella, and simultaneously, the mixed fermentation generates tiny proteins or peptides (antibacterial peptides) similar to bacteriocin such as various lactobacillin and bifidobacterin for staphylococcus, The clostridium, salmonella and shigella have antagonistic action and bacteriolytic action, and the lactic acid bacteria can improve water quality and inhibit vibrio in water, and can eliminate nitrite in water through direct chemical action and indirect denitrification promoting action of microorganisms, thereby achieving the effect of purifying water quality.
Detailed Description
The present invention is further described in the following examples, which are intended to be illustrative only, and the scope of the invention is not to be limited thereto, and all equivalent modifications which can be made by those skilled in the art based on the spirit of the present invention will fall within the scope of the present invention.
A mixed fermentation microbial deodorant is prepared from the following raw materials: the fruit flavor comprises probiotics, a culture medium and flavor fruits, wherein the flavor fruits can be one or more of apples, tangerines, pineapples and the like, and the probiotics comprise saccharomycetes, bacillus subtilis, bacillus licheniformis, photosynthetic bacteria, nitrobacteria, denitrifying bacteria, actinomycetes, sulfurous bacteria, aspergillus niger, aspergillus oryzae and lactic acid bacteria.
Specifically, the culture medium comprises cane molasses, peptone, maltooligosaccharide, magnesium sulfate, manganese sulfate, citric acid, dipotassium hydrogen phosphate, sodium acetate, beef extract, yeast extract, glycerol, tween 80, sodium chloride, calcium chloride, sodium hydroxide, potassium dihydrogen phosphate and water which are used in combination.
Specifically, the lactobacillus comprises bifidobacterium, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus.
Example one
The preparation method of the mixed fermentation microbial deodorant powder provided by the embodiment comprises the following steps:
s1: adopting a 5T fermentation tank, and cleaning and steam sterilizing the fermentation tank;
s2: injecting 3.5T water into the fermentation tank, and simultaneously starting a steam generator to heat the water body in the fermentation tank to 35 ℃;
s3: adding 150 kg of cane molasses, 30 kg of peptone, 300 kg of apples and 10 kg of malto-oligosaccharide into the fermentation tank heated in the step S2, and simultaneously starting a stirring device;
s4: adding manganese sulfate, citric acid, sodium acetate, magnesium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and sodium hydroxide into the fermentation tank, wherein the addition amount is 0.1% of the total amount, and adjusting the ph of the liquid culture medium to be 6.5;
s5: when the temperature in the fermentation tank rises to 35 ℃, stopping heating, firstly feeding bacillus subtilis such as aerobic bacteria, nitrobacteria, actinomycetes, sulfurous bacteria, aspergillus niger and aspergillus oryzae, then feeding saccharomycetes, bacillus licheniformis and denitrifying bacteria in facultative bacteria, finally feeding photosynthetic bacteria and lactic acid bacteria in anaerobic bacteria, testing the pH value, and adjusting the initial pH value to be between 6.5; the aerobic bacteria are bacillus subtilis, nitrobacteria, actinomycetes, sulfureted bacteria, aspergillus niger and aspergillus oryzae, the facultative bacteria are saccharomycetes, bacillus licheniformis and denitrifying bacteria, and the anaerobic bacteria are photosynthetic bacteria and lactic acid bacteria;
s6: after all probiotics are put in, the stirring can be stopped after the stirring is continuously carried out for 24 hours at a low speed (32r/min) in a closed state;
s7: fermenting for 48-52 hours to obtain a fermented mature microbial deodorant finished product;
s8: and filtering and canning the obtained fermented and mature microbial deodorant finished product.
Example 2
The preparation method of the mixed fermentation microbial deodorant powder provided by the embodiment comprises the following steps:
s1: adopting a 5T fermentation tank, and cleaning and steam sterilizing the fermentation tank;
s2: injecting 3T water into the fermentation tank, and simultaneously starting a steam generator to heat the water body in the fermentation tank to 35 ℃;
s3: adding 200 kg of cane molasses, 60 kg of peptone, 400 kg of pineapple and 20 kg of malto-oligosaccharide into the fermentation tank heated in the step S2, and simultaneously starting a stirring device;
s4: then adding manganese sulfate, beef extract, sodium hydroxide, yeast extract, tween 80, sodium chloride and citric acid into the fermentation tank, wherein the addition amount is 0.05 percent of the total amount, and adjusting the ph of the liquid culture medium to be 7.2;
s5: when the temperature in the fermentation tank rises to 35 ℃, stopping heating, firstly adding bacillus subtilis such as aerobic bacteria, nitrobacteria, actinomycetes, sulfurous bacteria, aspergillus niger and aspergillus oryzae, then adding saccharomycetes, bacillus licheniformis and denitrifying bacteria in facultative bacteria, finally adding photosynthetic bacteria and lactic acid bacteria in anaerobic bacteria, testing the ph value, and adjusting the initial ph value to 7.2; the aerobic bacteria are bacillus subtilis, nitrobacteria, actinomycetes, sulfurous bacteria, aspergillus niger and aspergillus oryzae, the facultative bacteria are saccharomycetes, bacillus licheniformis and denitrifying bacteria, and the anaerobic bacteria are photosynthetic bacteria and lactic acid bacteria;
s6: after all probiotics are put in, the stirring can be stopped after the stirring is continuously carried out for 24 hours at a low speed (32r/min) in a closed state;
s7: fermenting for 55-62 hours to obtain a fermented mature microbial deodorant finished product;
s8: and filtering and canning the obtained fermented and mature microbial deodorant finished product.
Example 3
The preparation method of the mixed fermentation microbial deodorant powder provided by the embodiment comprises the following steps:
s1: adopting a 5T fermentation tank, and cleaning and steam sterilizing the fermentation tank;
s2: injecting 3.5T water into the fermentation tank, and simultaneously starting a steam generator to heat the water body in the fermentation tank to 35 ℃;
s3: adding 140 kg of cane molasses, 40 kg of peptone, 250 kg of citrus and 5 kg of malto-oligosaccharide into the fermentation tank heated in the step S2, and simultaneously starting a stirring device;
s4: adding manganese sulfate, glycerol, tween 80 and citric acid into the fermentation tank, wherein the addition amount is 0.01% of the total amount, and adjusting the ph of the liquid culture medium to be 6.9;
s5: when the temperature in the fermentation tank rises to 35 ℃, stopping heating, firstly feeding bacillus subtilis such as aerobic bacteria, nitrobacteria, actinomycetes, sulfurous bacteria, aspergillus niger and aspergillus oryzae, then feeding saccharomycetes, bacillus licheniformis and denitrifying bacteria in facultative bacteria, finally feeding photosynthetic bacteria and lactic acid bacteria in anaerobic bacteria, testing the pH value, and adjusting the initial pH value to 6.9; the aerobic bacteria are bacillus subtilis, nitrobacteria, actinomycetes, sulfurous bacteria, aspergillus niger and aspergillus oryzae, the facultative bacteria are saccharomycetes, bacillus licheniformis and denitrifying bacteria, and the anaerobic bacteria are photosynthetic bacteria and lactic acid bacteria;
s6: after all probiotics are put in, the stirring can be stopped by continuously stirring for 24 hours at a low speed (32r/min) in a closed state;
s7: fermenting for 65-72 hours to obtain a fermented and mature microbial deodorant finished product;
s8: and filtering and canning the obtained fermented and mature microbial deodorant finished product.
Examples of the experiments
After 1L of sewage is prepared and the microbial deodorant prepared in example 1 is applied for 0.5h, the degradation rate of ammonia reaches 90.1%, the degradation rate of hydrogen sulfide reaches 88.9%, the degradation rate of styrene reaches 92.1%, the odor concentration is reduced from 549 before use to 19%, and the odor is degraded by 96.5%.
After 1L of sewage was prepared and the microbial deodorant of example 2 was applied for 0.5 hour, the degradation rate of ammonia reached 98.6%, the degradation rate of hydrogen sulfide reached 93.8%, the degradation rate of styrene reached 92.6%, the odor concentration decreased from 553 before use to 25%, and the odor was degraded by 95.4%.
After 1L of sewage was prepared and the microbial deodorant of example 3 was applied for 0.5 hour, the hydrogen sulfide was reduced by 90.2%, the odor concentration was reduced from 531 to 10 before use, and the odor was degraded by 98.3%.
The mixed fermentation microbial deodorant is a product obtained by mixing a plurality of different probiotics together and fermenting by sharing a culture medium, is also called mixed fermentation, can use the combination of different metabolic capacities of the plurality of probiotics to complete the complex metabolic action which is difficult to complete by a single probiotic, can replace certain recombinant engineering bacteria to perform complex various metabolic reactions, improves the production efficiency, and has many advantages that the pure culture is difficult to achieve; various mixed bacteria generate various active substances such as organic acid, special enzyme system, acidomycin, hydrogen peroxide and the like through fermentation to inhibit the growth of putrefying bacteria, wherein the hydrogen peroxide inhibits and kills gram-negative bacteria, escherichia coli and salmonella. Meanwhile, the mixed bacteria fermentation produces tiny proteins or peptides (antibacterial peptides) similar to bacteriocins, such as various lactobacillin and bifidus bacterin, and the mixed bacteria fermentation has antagonistic action and bacteria dissolving action on staphylococcus, clostridium, salmonella and shigella, and the lactic acid bacteria can improve water quality and inhibit vibrios in water body, and can eliminate nitrite in the water body through direct chemical action and indirect denitrification promoting action of microorganisms, thereby achieving the effect of purifying water quality.
The embodiments described above are preferred embodiments of the present invention, and not all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (9)
1. A mixed fermentation microbial deodorant is characterized by being prepared from the following raw materials: probiotics and culture medium, wherein the probiotics comprise saccharomycetes, bacillus subtilis, bacillus licheniformis, photosynthetic bacteria, nitrobacteria, denitrifying bacteria, actinomycetes, sulfurous bacteria, aspergillus niger, aspergillus oryzae and lactic acid bacteria.
2. The microbial deodorant according to claim 1, wherein the culture medium comprises a combination of plural kinds of cane molasses, peptone, malto-oligosaccharide, magnesium sulfate, manganese sulfate, citric acid, dipotassium hydrogen phosphate, sodium acetate, beef extract, yeast extract, glycerin, tween 80, sodium chloride, calcium chloride, sodium hydroxide, potassium dihydrogen phosphate, and water.
3. The microbial deodorant according to claim 1, wherein the lactic acid bacteria are lactic acid bacteria of the Bifidobacterium genus, Lactobacillus plantarum, enterococcus faecalis, Lactobacillus acidophilus.
4. The mixed fermentation microbial deodorant according to claim 1, wherein: the raw materials also contain fragrant fruits such as Mandarin orange, fructus Mali Pumilae, and fructus Ananadis Comosi.
5. A preparation method of mixed fermentation microbial deodorant powder is characterized by comprising the following steps:
s1: cleaning the fermentation tank and carrying out steam sterilization;
s2: injecting water into the fermentation tank, and simultaneously starting a steam generator to heat the water body in the fermentation tank;
s3: adding cane molasses, peptone, fragrant fruits and malto-oligosaccharide into the fermentation tank heated in the step S2, and simultaneously turning on a stirring device;
s4: then adding one or more of manganese sulfate, citric acid, sodium acetate, magnesium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and sodium hydroxide into the fermentation tank;
s5: when the temperature in the fermentation tank rises to 35 ℃, stopping heating, screening probiotics according to the using direction of the deodorant, firstly feeding aerobic bacteria, then feeding facultative bacteria, and finally feeding anaerobic bacteria, testing the pH value, and adjusting the initial pH value to 6.5-8; the aerobic bacteria are bacillus subtilis, nitrobacteria, actinomycetes, sulfurous bacteria, aspergillus niger and aspergillus oryzae, the facultative bacteria are saccharomycetes, bacillus licheniformis and denitrifying bacteria, and the anaerobic bacteria are photosynthetic bacteria and lactic acid bacteria;
s6: after all probiotics are put in, the stirring can be stopped by continuously stirring for 24 hours at a low speed (32r/min) in a closed state;
s7: fermenting for 48-72 hours to obtain a fermented and mature microbial deodorant finished product;
s8: and filtering and canning the obtained fermented and mature microbial deodorant finished product.
6. The method for preparing a mixed fermentation microbial deodorant powder according to claim 5, characterized in that: the water used in S1 is groundwater or purified water, and the water temperature is heated to 35 ℃.
7. The method for preparing the microbial deodorant powder fermented by mixed bacteria according to claim 5, characterized in that: the addition amount of manganese sulfate, citric acid, sodium acetate, magnesium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and NaOH in the S4 is 0.01-0.1% of the total amount, and the pH value of the liquid culture medium is adjusted to 6.5-7.2.
8. The method for preparing a mixed fermentation microbial deodorant powder according to claim 5, characterized in that: in the step S5, the aerobic bacteria are put in 48 hours, and then the anaerobic bacteria are put in.
9. The method for preparing the microbial deodorant powder fermented by mixed bacteria according to claim 5, characterized in that: and in the S7, the ph value is used for identifying whether the fermentation is mature, and the ph value of the fermentation mature is between 3 and 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210572085.5A CN114920364A (en) | 2022-05-24 | 2022-05-24 | Mixed-strain fermentation microbial deodorant and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210572085.5A CN114920364A (en) | 2022-05-24 | 2022-05-24 | Mixed-strain fermentation microbial deodorant and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114920364A true CN114920364A (en) | 2022-08-19 |
Family
ID=82811452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210572085.5A Pending CN114920364A (en) | 2022-05-24 | 2022-05-24 | Mixed-strain fermentation microbial deodorant and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114920364A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115948286A (en) * | 2022-11-28 | 2023-04-11 | 郑州昌诺机电科技有限公司 | Microbial agent for anhydrous closestool base material and preparation process |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101029346B1 (en) * | 2009-10-28 | 2011-04-13 | 주식회사 엠투원 | Combined microorganism fermented liquors having the effect of reducing odor gas and antibacterial effect, and methods of using the same |
| CN102578156A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof |
| CN105132300A (en) * | 2015-09-30 | 2015-12-09 | 湖北金润德环保技术有限公司 | Method for preparing natural water ecological purification microbial inoculum |
| CN108251332A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN108310962A (en) * | 2018-04-12 | 2018-07-24 | 北京昊业同宇科技有限公司 | Preparation method, deodorant and its application method of complex microorganism deodorant |
| CN108404647A (en) * | 2018-03-27 | 2018-08-17 | 湖南省蓝正微生物科技开发有限公司 | Complex microorganism deodorant |
| CN109607818A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of microorganism improver of water quality and preparation method thereof |
| CN110591966A (en) * | 2019-09-30 | 2019-12-20 | 四川明湖环保科技有限公司 | Environment deodorization composite microbial inoculum and preparation method thereof |
-
2022
- 2022-05-24 CN CN202210572085.5A patent/CN114920364A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101029346B1 (en) * | 2009-10-28 | 2011-04-13 | 주식회사 엠투원 | Combined microorganism fermented liquors having the effect of reducing odor gas and antibacterial effect, and methods of using the same |
| CN102578156A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof |
| CN105132300A (en) * | 2015-09-30 | 2015-12-09 | 湖北金润德环保技术有限公司 | Method for preparing natural water ecological purification microbial inoculum |
| CN108251332A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN108404647A (en) * | 2018-03-27 | 2018-08-17 | 湖南省蓝正微生物科技开发有限公司 | Complex microorganism deodorant |
| CN108310962A (en) * | 2018-04-12 | 2018-07-24 | 北京昊业同宇科技有限公司 | Preparation method, deodorant and its application method of complex microorganism deodorant |
| CN109607818A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of microorganism improver of water quality and preparation method thereof |
| CN110591966A (en) * | 2019-09-30 | 2019-12-20 | 四川明湖环保科技有限公司 | Environment deodorization composite microbial inoculum and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 黄仁术 等: "几种除臭微生物的高效组合筛选", 湖北农业科学, vol. 50, no. 4, pages 2844 - 2846 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115948286A (en) * | 2022-11-28 | 2023-04-11 | 郑州昌诺机电科技有限公司 | Microbial agent for anhydrous closestool base material and preparation process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102177670B1 (en) | Composition for removing odor and, manufacturing method thereof | |
| US6025187A (en) | Combinations of bacillus and lactobacillus species for producing protein from waste | |
| KR101946461B1 (en) | A preparation for producing of fermented liquid fertilizer with effect reducing odor of livestock waste, and fermented liquid fertilizer device for reducing odor of livestock waste using the same | |
| KR102174591B1 (en) | Treating method and treating equipment for Food Waste | |
| KR101734140B1 (en) | Composition for reducing odor gas with antimicrobial effect and preparation method thereof | |
| CN108456650A (en) | A kind of complex microorganism deodorizing microorganism and its preparation method and application | |
| CN102258796A (en) | Microbial deodorant and preparation method thereof | |
| CN109182157A (en) | A kind of composite bacteria preparation and preparation method thereof improving breeding environment | |
| WO2023217056A1 (en) | Composite microbial deodorant, method for preparing same, and use thereof | |
| CN111903838A (en) | Yeast culture and compound lactobacillus preparation and preparation method thereof | |
| CA2555516A1 (en) | Food additive for human and animal and uses thereof | |
| CN109182177A (en) | A kind of compound probiotic deodorant and the preparation method and application thereof | |
| US4264448A (en) | Method for bacteriological treatment of manure and high bod industrial wastes | |
| KR20150066228A (en) | A method of organic waste treatment using microbial carriers | |
| US11680003B2 (en) | Treatment of animal and poultry waste to reduce odor | |
| KR101191326B1 (en) | Method for treating organic waste using carrier | |
| CN109603514A (en) | A kind of microbial deodorant and preparation method thereof | |
| CN114920364A (en) | Mixed-strain fermentation microbial deodorant and preparation method thereof | |
| CN108611301B (en) | Composite biological deodorant and preparation method thereof | |
| AU2017211260B2 (en) | A process for the production of a bacterially enriched animal feed composition | |
| KR20180105593A (en) | Microbial agent having decomposition of organic waste and excellent deodorization and its manufacturing method | |
| JP2010088310A (en) | Microorganism having organic waste-decomposing action, microorganism composition, decomposing method of organic waste, and manufacturing method of compost | |
| CN107373024A (en) | Animal feed additive and its preparation method and application | |
| KR101993762B1 (en) | Liquefied deodorant and fabricating method thereof | |
| JP5445821B2 (en) | Microorganisms exhibiting organic waste decomposing action, microbial composition, organic waste decomposing method, and compost producing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |