KR101946461B1 - A preparation for producing of fermented liquid fertilizer with effect reducing odor of livestock waste, and fermented liquid fertilizer device for reducing odor of livestock waste using the same - Google Patents

Abstract

The present invention relates to an agent for producing fermented liquid fertilizer capable of reducing the odors of livestock manure; and a fermented liquid fertilizer circulation apparatus for reducing the odors of livestock manure using the same. In particular, the agent for producing fermented liquid fertilizer capable of reducing the odors of livestock manure comprises a novel Bacillus kochii strain 4584 (KCTC 13552BP) which is formed of a base sequence represented by SEQ ID NO: 1 and has the effect of reducing the odors of livestock manure. According to the present invention, the fermented liquid fertilizer produced through mass propagation of nitrifying bacteria is continuously circulated to the slurry pit of a livestock house, resulting in domination of beneficial bacteria inside the slurry pit of the livestock house, thereby effectively reducing the odors of manure generated inside the livestock house as well as propagation of harmful bacteria, and accordingly the rearing environment in the livestock house may be dramatically improved. Furthermore, as a liquid phase, which is produced from turning the livestock manure in 120-150 days amount of manure produced daily into liquid fertilizer by using beneficial bacteria, is circulated through a pigpen and a fermenter without propagation of harmful bacteria and without the separate use of disinfectants or antibiotics commonly used, the odors of livestock manure can be eliminated or reduced in a rapid and economic manner.

Description

FIELD OF THE INVENTION The present invention relates to a non-fermented liquid fermented liquid fertilizer, and a fermented liquid fertilizer device for reducing odor of a livestock manure. of livestock waste using the same}

The present invention relates to a preparation for fermentation broth production having an effect of reducing odor of livestock manure, and a non-decaying circulation apparatus for fermentation broth for reducing odor of livestock manure using the same. More particularly, And then circulating the microorganism to the housing slurry pit to ignite the nitrifying bacteria microorganisms remaining in the housing slurry pit to reduce odor of the manure generated in the housing, It is possible to proliferate beneficial microorganisms of nitrifying bacteria even in the basic amount of disinfectant or antibiotic used normally without using sludge decomposition effect and proliferation of harmful microorganisms, thereby improving odor and environment of livestock manure And the fermentation broth is circulated Industrial use, to a livestock manure odor reduction for fermented non-volatile circulator capable of economically removed or reduced.

Livestock Environment Improvement Organization The Livestock Environment and Technology Research Institute recently summarized the problem of odor in the livestock facilities with a large number of complaints and the countermeasure technology. Complaints related to odors account for about 60% of civil complaints stemming from animal husbandry management. Measures against odors are an urgent task to be solved by one of the livestock environmental problems.

The difficulty of countermeasures in the livestock industry is that the source of the odor is wide, the odor is difficult to collect, and because it is a complex odor, a technique capable of responding to odor substances having different properties is required. At low concentrations, the air volume is large, and the concentration and air volume vary greatly depending on the source.

The cost of measures against odors does not contribute to the production of livestock products. On the other hand, when costs are incurred, the management burden is caused.

In Japan, deodorization technology has been developed for odors generated in sewage treatment facilities. The deodorization system using bio-odor method using microorganisms is an effective technology as a countermeasure for civil complaints. However, since the facility cost is required, it is introduced to a joint use type composting center and a place where complaints are required.

On the other hand, the stench ventilation odor has recently become a target of complaints due to changes in the environment surrounding the livestock farms, and it is necessary to take measures against odor of low concentration and large amount of air such as housing environment. In Europe and the United States, there are many barns and cages in the barn house.

A related art is Korean Patent No. 10-1120095. The patent discloses a method of passing citrus wood sawdust and manure composting together with odor-reducing microorganisms to pass or adsorb malodorous odor.

Korean Patent Laid-Open Publication No. 10-2012-0068194 also discloses a method for producing microbial cells comprising a mixture of bamboo leaves and pine needles, indigenous microorganisms consisting of Lactobacillus parakefih and Candida glabrata, and anhydrous glucose Discloses a method for removing odor of an animal manure by mixing the composition with an animal manure fertilizer in an amount of 10 to 30% by weight. However, in the case of the above-mentioned patent, the treatment efficiency of artificially cultivated microorganisms shows a large variation according to the degree of rotting of the manure, and the material used for reducing the odor of the livestock manure is difficult to supply and is not suitable for large-scale livestock manure treatment .

Korean Patent No. 10-0378667 discloses a method of mixing a composition of a malodor decomposing microorganism with 5 to 10% livestock manure, mixing them with sawdust, rice husk, and litter powder to aerobically ferment the compost.

Korean Patent No. 10-0848677 discloses a method for producing a microorganism which comprises 30 to 50 parts by weight of humus earth, 10 to 30 parts by weight of wood clay, 5 to 20 parts by weight of burnt lime, 5 to 20 parts by weight of lung fossil, 0.5 to 2 parts by weight of a starch is mixed and fermented at a temperature of 50 to 60 for 5 to 7 weeks. However, in the case of the patent, the amount of excipients to be processed is large compared to the amount of manure to be treated, which is very inefficient and requires mechanical equipment investment required for large-scale treatment, which is impractical. Therefore, in order to efficiently treat livestock manure, development of a method for eliminating or reducing the odor of livestock manure more quickly and economically is required.

Korean Registered Patent No. 10-1120095 " Korean Patent Publication No. 10-2012-0068194 'Composition for Removing Odor from Livestock Manure, Fertilizer Using the Same, Korean Patent No. 10-0378667 'Rapid desizing and composting method of livestock manure and the like and composition thereof' Korean Patent No. 10-0848677 'Method of producing compost'

It is an object of the present invention to reduce the odor of manure generated in a housing and to prevent the disinfection through degradation of sludge and the proliferation of harmful microorganisms as the animal manure is circulated for 120 days or more, A non-fermented liquid manufacturing preparation having an odor reduction effect of an animal manure which can remove or reduce the odor and environment improvement of the livestock manure more quickly and economically by allowing nitrification bacteria microorganisms to grow in the basic amount, And to provide a non-decaying circulation apparatus for a fermentation broth.

In order to accomplish the above object, the present invention provides a fermentation broth preparation preparation having an odor reduction effect of an animal manure of the present invention, which comprises a nucleotide sequence represented by SEQ ID NO: 1 and has a microbial Bacillus cochlea kochii strain 4584 (KCTC 13552BP) or a culture thereof.

The preparation is also useful as a nitrifying bacteria useful microorganism, such as Bacillus psychrodurans , Staphylococcus pasteuri , Bacillus velezensis , Geobacillus stearothermophilus , Bacillus binary Shen aft of, but containing a mixed culture, including any further one or more, and containing a culture of the mixed culture as an active ingredient, more preferably Lactobacillus Kasei (Lactobacillus as the nitrification bacteria beneficial microorganisms (bacillus ginsengihumi) casei), trophy kusu (bacillus methylotrophicus) of the Bacillus methyl, Lactobacillus parapa than varnish (Lactobacillus parafarraginis), Bacillus subtilis (Bacillus subtilis), acetonitrile bakteo Rover you N-Sys (Acetobacter lovaniensis), Bacillus amyl Lowry query Pacific Enschede (bacillus amyloliquefaciens ) is further added. It is Jing.

In another aspect of the present invention, there is provided a fermentation liquid circulation apparatus for reducing animal offal odor, comprising an animal manure transported from a housing slurry pit (10), a fermentation broth preparation preparation and a chelated phyllosilicate as a carbon source, %, And mixing the mixture; An aerobic fermentation liquid non-aerobic tank 200 for fermenting an animal manure mixed with the preparation for fermentation non-production supplied from the raw water tank 100 to produce an aerobic fermentation liquid ratio; And a fermentation liquid non-feeding pipe (300) for feeding the aerobic fermentation broth of the aerobic fermentation broth (200) to the housing slurry pit (10), wherein the aerobic fermentation broth ratio supplied to the housing slurry pit (10) Is mixed with manure and is supplied to the housing slurry pit 10 through the raw water tank 100, the aerobic fermentation liquid non-feeding tank 200 and the fermentation liquid non-feeding pipe 300, and is repeatedly circulated.

The aerobic fermentation liquid non-aerobic tank 200 is composed of four fermentation tanks 220, 230, 240 and 250, a sedimentation tank 260 and an active water tank 270.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. It will be possible.

According to the present invention, the fermentation broth produced by the massive proliferation of the microorganisms capable of nitrifying bacteria is continuously circulated by the slurry pits of the housing, so that the useful microorganisms in the housing slurry pit are ignited and the odor of the manure generated in the housing is effectively reduced As the propagation of harmful microorganisms is also suppressed, the environment for housing is greatly improved.

In addition, since the liquid manure that is livable as the beneficial microorganism is circulated through the pesticide and the fermentation tank, the livestock manure over 120 days ~ 150 days of the daily manure amount is circulated. Therefore, without the addition of the disinfectant or the antibiotic which is conventionally used without the growth of harmful microorganisms, The odor of the manure can be removed or reduced more quickly and economically.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a view showing an apparatus for circulating fermentation broth for reducing odor in an animal manure using a fermentation broth preparation according to Example 1 of the present invention. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Detailed descriptions of well-known functions and constructions that may unnecessarily obscure the gist of the present invention will be omitted.

1, the fermentation liquid non-circulating apparatus for reducing odor of an animal manure according to the present invention comprises a preparation for fermentation broth non-manufacturing preparation conveyed from a microorganism incubator 280 to an animal manure conveyed from a housing slurry pit 10, Fermented livestock manure mixed with a raw water tank 100 in which chelated phyllotaxis is added in an amount of 2 to 5% by weight based on the weight of the preparation and mixed with a preparation for fermentation broth non-production supplied from the raw water tank 100 And an aerobic fermentation liquid non-aerobic tank (200) for producing aerobic fermentation liquid ratio and a fermentation liquid non-feed pipe (300) for feeding the aerobic fermentation liquid ratio of the aerobic liquefaction tank to the housing slurry pit. The fermentation liquid ratio is re-mixed with the livestock manure and supplied again to the housing slurry pit 10 through the raw water tank 100, the aerobic fermentation liquid non-feeding tank 200 and the fermentation liquid non-feeding pipe 300 to be repeatedly circulated It is characterized.

At this time, 2 to 5% of the chelated phyllotaxis is injected into the carbon source as an effective ingredient of the non-fermentation broth preparation, and useful microorganisms are proliferated at a high rate. In addition, the odor of the housing is effectively reduced due to the addition of specific nitrifying bacteria microorganisms, and in particular, the fermentation liquid non-circulating device for reducing the odor of the animal manure according to the present invention has the advantage that the manure The environment of the poultry slurry pit 10 is improved as the liquid pellets and the fermentation livestock manure sludge are recycled as the beneficial microorganism is circulated, so that the poultry manure can be sterilized without additional use of disinfectant or antibiotics commonly used without proliferation of harmful microorganisms Can be removed or reduced more quickly and economically.

In the present invention, the treatment for livestock manure over 120 days to 150 days of daily manure amount is proposed. However, if the amount of useful microorganism is increased by 2 times, 3 times, 10 times or more, , And it is also possible to treat livestock manure in a short period of time with a smaller amount of input.

Hereinafter, as one embodiment of the fermentation liquid non-circulating device for reducing odor of an animal manure of the present invention, there is provided an effective ingredient of a specific fermentation broth preparation preparation used in a fermentation liquid non- circulating device for reducing odor of an animal manure Specifically, it is as follows.

(Major components of fermentation liquid circulation system for odor reduction in livestock manure)

1) The housing slurry pit 10, the livestock manure transfer pipe 30, the solid-liquid separator 20,

When the state of livestock manure stored in the housing slurry pit 10 is examined, COD is 30,000 to 40,000 ppm, SS is 50,000 to 60000 ppm, and TN is the amount of livestock manure discharged from the housing slurry pit 10, 9000 to 10000 ppm.

The livestock manure collected in the housing slurry pit 10 is conveyed to the raw water tank 100 through the livestock manure conveying pipe 30 and the solid liquid separator 20 is installed in the middle of the livestock manure conveying pipe 30 , The solid livestock manure and the liquid livestock manure filtrate are separated by the solid-liquid separator (20) and only the liquid livestock manure having the solid livestock manure (solid content) of 20% or more removed is transferred to the raw water tank (100) do. At this time, the solid-liquid separated solid matter is collected at least once a day, composted and composted, and the liquid livestock manure has a COD of 15,000 to 20,000 ppm, a SS of 25,000 to 30,000 ppm, and a T-N of 8,000 to 8,500 ppm.

2) The raw water tank 100, the microorganism incubator 280,

The liquid fermented manure stored in the raw water tank 100 is mixed with a specific fermentation broth preparation preparation and carbon source fed through the microorganism incubator 280 and mixed. At this time, 2 to 5% by weight, based on the weight of the livestock manure, It is characterized by the rapid growth of useful microorganisms in accordance with the consumption of nitrogen and coal. In addition, humic acid is added to the raw water tank 100 as a carbon source in an amount of 2 to 5% by weight based on the weight of the livestock manure, thereby contributing to the growth of the beneficial microorganism.

Herein, the chelated phyllite is referred to herein as being produced so as to increase the absorption capacity of the phyllite by pulverizing the pulverized particles of phyllite. The phyllite rocks have hydrolytic activity by hydrothermal alteration and / It is an ore produced through processes such as denaturation, weathering, and corrosion, and various minerals are appropriately mixed. Since the nano-sized fine spaces are formed in the phyllite rock, the physical adsorption power and the chemical cationic cyanide action are very excellent. The content of germanium (ge) is 0.11ppm, which is higher than the germanium content of ginseng and hot spring water. It also neutralizes or removes toxic substances by physical adsorption of phyllite and chemical cationic action.

Humic acid is a high molecular substance having a molecular weight of 10,000 to 100,000 which is solidified in an acidic condition and liquidified under an alkaline condition. The humic acid improves digestion and absorption to improve nutrition absorption, promotes circulation of blood, It is known that it has the function of dissolving constituent molecular protein and carbohydrate of substance and decomposing it and killing it and excreting it out of the body, and acting to prevent decay and oxidation.

When the weight of each of the chelated phyllotoxins or the humic acid is less than 2% by weight, it is difficult to obtain the ability to multiply the beneficial microorganism according to the addition. If the weight of the chelated phyllotactic acid or humic acid exceeds 5% by weight, The productivity of the useful microorganisms is lowered in each of the following fermentation tanks, so that there is a possibility that the increased effect due to the addition of the additional substances may not be obtained.

In addition, the useful microorganism incubator (280) includes a compound useful microorganism preparation for producing a specific fermentation broth having an odor reducing effect of an animal manure, wherein the effective ingredient of the preparation for non fermentation broth is selected from the group consisting of The culture of the mixture is applied.

The nitrifying microorganism used herein is characterized by using a novel microorganism Bacillus kochii strain 4584 (KCTC 13552BP), which is composed of the nucleotide sequence shown in SEQ ID NO: 1 and has an odor reducing effect on animal manure .

In other words, the inventors of the present invention have found that the new microorganism has excellent odor reduction effect on animal manure, and the inventors of the present invention have found that such a Bacillus cochii strain was introduced into Korean Institute of Biotechnology (KRIBB) on June 20, (Accession No .: KCTC 13552BP).

In addition, the nitrifying bacteria microorganism may be added to the new microorganism Bacillus kochii strain 4584 (KCTC 13552BP) with Bacillus psychrodurans , Staphylococcus pasteuri , Bacillus cereus, Bacillus velezensis , Geobacillus stearothermophilus , and bacillus ginsengihumi . More preferably, the mixed strains may be composed of Bacillus amylori A bacterium belonging to the genus Bacillus such as bacillus amyloliquefaciens , Lactobacillus casei , bacillus methylotrophicus , Lactobacillus parafarraginis , Bacillus subtilis , one member selected from (Acetobacter lovaniensis) And also to add more configurations.

Although the above-mentioned specific nitrifying bacteria-use microorganism-mixed strains have been mentioned to date to promote the growth of crops, there is no use of the malodor reducing ability, and as the mixture is in the above mixture state, So that the desired effect of the present invention can be obtained.

As used herein, the term culture may mean a substance including all the substances contained in a medium cultured with the strain, for example, a substance including a metabolite or secretion resulting from the culture of the strain, or a lysate thereof The strain itself may also be contained in the culture. The culture can also be cultured according to a method commonly used in the art.

The medium can be a conventional medium, and the nitrifying bacteria can be cultured at 23 to 35 ° C for 92 to 240 hours to prepare a culture of the useful microorganism mixture at a concentration of 1 × 10 6 CFU / ml or more , More preferably all of the above-mentioned nitrifying bacteria microorganism strains are inoculated in the same amount, and then aeration amount of 0.05 to 0.4 vvm (vol / vol / min.) At 20 to 35 ° C for 100 to 120 hours, 100 to 200 rpm (rotary / min.) And an internal pressure of 0.1 to 1 kgf / cm 2, and the pH of the medium is most preferably 6.0 to 9.0.

The fermentation broth preparation preparation thus prepared may contain 100% by weight of the culture or may further comprise an excipient. In the case of the excipient, a carbon source or a nitrogen source or a mixture thereof may be used, and starch, amino acid, vitamin C, vitamin E, glucose and glycerin may be used singly or in combination of two or more. . The starch may be used to prevent film formation due to sugar components. The starch may be used in an amount of 10 to 20% by volume, but is not limited thereto. When the amount of starch is increased, microorganisms are diluted and the total number of bacteria is lowered. It can be used for pH control of vitamin C (ascorbic acid) and for vitamin use. The vitamin C may be used in an amount of 0.1 to 0.5% by volume, but is not limited thereto. The vitamin C may affect the growth and activity of the strain by excessively decreasing the pH at the time of overdose. The glucose or glycerin may also be used to stabilize microorganisms.

The thus prepared fermentation broth preparation preparation is stored and supplied to the microorganism incubator 280. The microorganism incubator 280 quickly stabilizes the aerobic fermentation broth 200 in the initial stage, The odor reduction of the housing and the improvement of the environment are influenced by the activity of the mixed strains provided in the microorganism incubator 280.

Described below is how the aerobic fermentation broth ratio is supplied to the housing slurry pit 10 and how much microbial activity is achieved in the present invention. Therefore, it is important to maintain a balance between the amount of animal manure produced and the ability to treat microorganisms, and it is very important to manage microorganisms through the microorganism incubator 280.

The fermentation broth preparation preparation is mixed with the liquid animal manure in the raw water tank 100 so that the fermentation broth preparation preparation is mixed in an amount of 0.1 to 30% by weight based on the livestock manure weight By weight, and more preferably 1 to 20% by weight. If the microorganism preparation contains less than 0.1% by weight of the microorganism preparation, the malodor removing effect of the animal manure may be insignificant. When the microorganism preparation is more than 30% by weight, the effect is almost the same as that of the case.

Examples of the animal manure include but are not limited to mammals such as cows, pigs, sheep, goats, and poultry such as pheasants, chickens, ducks, and turkeys.

3) aerobic fermentation broth (200)

At least four aerobic fermentation broilers (200) are used. This is because at least four aerobic fermentation broths are continuously installed, the odor of livestock manure is effectively removed while controlling the reaction temperature and air supply The nitrification reaction is accelerated to improve the performance of the pot, and the harmful microorganisms are killed. More preferably, the aerobic fermentation broth (200) of the present invention uses 4 to 6 fermentation tanks, and it is difficult to obtain a certain number of bacteria when only 4 antibiotics are used. And when it is used in excess of 6, it is difficult to obtain the increased efficacy of the additional fermenter.

1, the aerobic fermentation broiler 220 according to an embodiment of the present invention includes a fermentation tank 220, 230, 240, 250, a sedimentation tank 260, and an active water tank 270 The livestock manure mixed with the preparation for fermentation broth preparation of the raw water tank 100 is manufactured by the fermentation tank 220, the fermentation tank 230, the fermentation tank 240, the fermentation tank 250 and the settling tank 260 in this order, The produced aerobic fermentation broth is separated from the remaining solids through the settling tank 260, and then stores the aerobic fermentation broth in a liquid form in the active water tank 270.

At this time, the fermentation tanks 220, 230, 240 and 250 stabilize the organic matter and the malodor component of the livestock manure mixed with the preparation for fermentation broth production under aerobic conditions, To supply oxygen for 1 hour.

The oxygen supply, that is, the aeration can be performed through the oxidizer, and the oxidizer can be installed in the fermentors 220, 230, 240 and 250 with high efficiency of oxygen transfer efficiency, It is good to reduce the power cost. Since the concentration of the organic substance is high even when the livestock manure is subjected to solid-liquid separation, a large amount of air is required to obtain a stable fermentation liquid ratio. Therefore, it is desirable to reduce the power ratio of the blower by using a high-efficiency diffuser. If the oxygen supply is adjusted according to the time of bubble control, it is not necessary to install the parcel.

In order to minimize the rate of bacterial mortality of the nitrifying bacteria microorganism mixture, which is an effective component of the non-fermentation broth preparation of the present invention, and to maximize their activity and reduce malodor, the fermentation tank 220 The reaction temperature is 30 ~ 1.0 × 10 ² In to a ^{50 ℃ ~ 9.0 × 10 2 CFU} / ml , and that nitrification bacteria beneficial microorganism mixture concentration is created, the reaction temperature of the fermentation tank 230 is 1.0 × 10 to a 30 ~ 50 ℃ ³ ~ 9.0 × 10 ⁴ CFU / ml and so that the nitrification bacteria beneficial microorganism mixture concentration is created, the reaction temperature of the fermentation tank 240, from 30 to a 50 ℃ to ^{1.0 × 10 5 ~ 9.0 × 10} 5 nitrification bacteria in the CFU / ml concentration And the reaction temperature of the final fermenter (250) is set at 40 to 60 ° C to produce a useful microorganism mixture of nitrifying bacteria at a concentration of 1.0 × 10 ⁶ to 9.0 × 10 ⁶ CFU / ml. Thus, the harmful microorganism Escherichia coli Or to kill Salmonella.

4) The fermentation liquid supply pipe 300, the circulation storage tank 400,

The fermentation liquid non-feeding pipe 300 supplies the aerobic fermentation liquid ratio of the active water tank 270 to the housing slurry pit 10 and circulates the aerobic fermentation liquid to the housing slurry pit 10, To be circulated.

As described above, the aerobic fermentation broth produced by massive growth of nitrifying bacteria microorganisms is continuously circulated to the housing slurry pit 10, whereby beneficial microorganisms in the housing slurry pit 10 are dominated, It is diluted and the proliferation of harmful microorganisms is suppressed, thus the odor is reduced and the breeding environment is greatly improved. In addition, by repeatedly circulating the aerobic fermentation broth through the housing slurry pit 10, degradation of the microorganism occurs, the animal manure is not rotted, the odor is reduced, and the breeding environment is drastically improved, thereby preventing disease and providing the optimal environment. Disease-improving effect and environmental friendliness can be realized.

In addition, the total treating capacity of the raw water tank 100 and the aerobic fermentation broth 200 can be stored for 120 days to 150 days based on 5 kg of the daily dose of 1 fermented milk per day And when the aerobic fermentation broth is not treated 3 months later, aerobic fermentation broth can be treated without solid-liquid separation of the waste manure. Therefore, after three months, the extracorporeal extinction is completed and the solid-liquid separator 20 is not necessary.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. However, these Examples and Experimental Examples are for illustrative purposes only and do not limit the scope of protection of the present invention.

<Example 1> Production of preparation 1 for non-fermentation broth having an odor reducing effect of the livestock manure of the present invention

A new microorganism Bacillus kochii strain 4584 (KCTC 13552BP) consisting of a nucleotide sequence represented by SEQ ID NO: 1 as a nitrifying bacteria microorganism and having an odor reduction effect of an animal manure was prepared, Cultured to prepare a culture product having a concentration of 1 × 10 8 CFU / ml to prepare Formulation 1 for preparation of non-fermented broth of the present invention containing it as an active ingredient.

Example 2 Production of a preparation 2 for fermentation broth production having an odor reducing effect of an animal manure of the present invention

A new microorganism Bacillus kochii 4584 strain (KCTC 13552BP), Bacillus psychrodurans , Staphylococcus faast strains, which is composed of the nucleotide sequence represented by SEQ ID NO: 1 and has an odor reducing effect on an animal manure, The same amount of nitrifying bacteria microorganisms of Staphylococcus pasteuri , Bacillus velezensis , Geobacillus stearothermophilus and bacillus ginsengihumi were mixed in the same amount, Cultured for 240 hours to prepare a culture at a concentration of 1 x 10 &lt; 8 &gt; CFU / ml to prepare Formulation 2 for the non-fermented broth preparation of the present invention containing it as an active ingredient.

<Example 3> Production of preparation 3 for non-fermented broth having an odor reduction effect of the livestock manure of the present invention

Useful microorganisms of Formulation 2 of Example 2 include Lactobacillus casei , bacillus methylotrophicus , Lactobacillus parafarraginis , Bacillus subtilis , Acetobacter lovaniensis and bacillus amyloliquefaciens were added in an equal amount to prepare Formulation 3 for the non-fermented liquid preparation of the present invention.

<Example 4> Use of a fermentation liquid non-circulating device for reducing odor of livestock manure using an agent 2 for preparation of fermentation broth having an odor reducing effect of an animal manure of the present invention

Each day, 5kg of pork per day was put into three places of slurry pit (10).

The money contained in three places of the housing slurry pit 10 is transferred to the raw water tank 100 through the livestock manure transferring pipe 30 so as to be transferred to the housing slurry pit 10 and the livestock manure transferring pipe 30, The separator 20 is provided to remove the solid content of the swine so that only liquid money is transferred to the circulating water tank 100.

Also, the preparation for fermentation broth preparation of Example 3, which is contained in the microorganism incubator 280, is also transferred to the raw water tank 100 to about 1 to 1.2 tons per 100 ton of the liquid form poultry, 120 liters were put together and mixed.

In the raw water tank 100 in which the liquid fodder, the fermentation broth preparation preparation, and the carbon source are mixed, the fermentation tanks 220 and 230 (hereinafter, referred to as &quot; fermentation tanks &quot;) for reducing the odor of the liquid feces and maximizing the activity of the non- 240) 250, a settling tank 260, and an active water tank 270 to produce an aerobic fermentation broth ratio.

The thus generated aerobic fermentation absorber is fed to the circulating storage vessel 400 through the fermentation liquid feed pipe 300 and then supplied to the housing slurry pit 10 again. And 5kg per day, so that the above process is repeatedly circulated to reduce the odor of swine, and at the same time, circulation treatment of the storage material of livestock manure for more than 120 days results in the disinfection of the sludge and the disinfectant or antibiotic The odor of livestock manure was removed without any additional use.

At this time, when the above-mentioned repeated circulation process is completed after treating the 120-minute money amount (about 3 months later), the aerobic fermentation broth can be treated without solid-liquid separation of the pig manure, do.

&Lt; Comparative Example 1 > Preparation of preparation 1 for non-fermented broth preparation

Lactobacillus casei , bacillus methylotrophicus , Lactobacillus parafarraginis , Bacillus subtilis , and Bacillus subtilis were cultivated in the same manner as in Example 2, , Acetobacter lovaniensis , and Bacillus amyloliquefaciens were used to prepare Formulation 1 for non fermentation broth.

&Lt; Comparative Example 2 > Preparation of agent 2 for preparation of fermentation broth

(Japanese Patent Application Laid-Open No. 10-1670651), and more particularly, to a method for producing Lactobacillus parafarrhinis Lactobacillus paracasei , Lactobacillus paracasei , Lactobacillus tolerans , Lactobacillus spp ., Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Needle N-Sys (Acetobacter lovaniensis), acetonitrile bakteo peroxy thiooxidans (Acetobacter peroxydans), Pichia peomen Tansu (Pichia fermentans), Candida ethanol Rica (Candida ethanolica), Saccharomyces Rommie Cobb cis Scoring Enigma (Saccharomycopsis schoenii), thio Bacillus thio oxy Providence (T hiobacillus thiooxidans ), Bacillus thuringiensis Bacillus thuringiensis , Weissella cibaria , Weissella paramesenteroides and Bacillus amyloliquefaciens were mixed and cultured at 25 ° C. for 120 hours A preparation 2 for preparation of a fermentation broth containing an effective microbial culture at a concentration of 1 x 10 &lt; ⁷ &gt; CFU / ml as an active ingredient was prepared.

<Experimental Example 1> Determination of odor reduction ability of livestock manure

1. Experimental Method

The preparation for fermentation broths of Examples 1, 2 and 3 and the preparation for fermentation broth of Comparative Examples 1 and 2 were added to the animal manure in an amount of 20% by weight based on the livestock manure weight, The gas concentration of hydrogen sulfide (H ₂ S) and the ammonia (NH ₃ ) gas concentration were measured during fermentation.

At this time, the concentration of the test gas was measured by a gas detection tube (formerly KS I 2218: 2009).

2. Experimental results

The results of the experiment are shown in Table 1 below.

Decrease of hydrogen sulfide (%) Ammonia reduction rate (%) Comparative Example 1 70.1% 65.2% Comparative Example 2 75.1% 70.9% Example 1 80.2% 80.0% Example 2 85.2% 85.0% Example 3 89.5% 89.6%

As shown in Table 1, it was confirmed that the preparation for preparation of non-fermented broths of Examples 1, 2, and 3 of the present invention exhibited a high odor-reducing ability in a short period of time even at a small number of bacteria.

&Lt; Experimental Example 2 >

1. Experimental Method

The malodor of the swine was reduced through the method of using the fermentation liquid non-circulating device for reducing animal offal odor in Example 4, and the activity of the non-fermentation liquid preparation preparation of Example 2 produced for each fermentation tank And the ammonia (NH ₃ ) gas concentration was measured). As a comparative example, a fermentation broth preparation preparation of Comparative Example 1 was used (Comparative Example 3) or a fermentation tank of the aerobic fermentation broth 200 was used in a circulation apparatus of Example 4, (Comparative Examples 4 and 5), and their activities were confirmed together.

2. Experimental results

The results of the experiment are shown in Table 2 below.

Bacteria of useful microorganism mixed strains Ammonia reduction rate (%) Whether E. coli or Salmonella is detected In the fermenter 220 of Example 4, 1.2 x 10 &lt; ² &gt; CFU / ml 85.2% none The fermenter (230) of Example 4 2.1 x 10 &lt; ³ &gt; CFU / ml 89.7% none In the fermenter (240) of Example 4, 2.2 x 10 &lt; ⁵ &gt; CFU / ml 92.3% none The fermenter (250) of Example 4 2.2 x 10 &lt; ⁶ &gt; CFU / ml 98.6% none Comparative Example 3 2.2 x 10 &lt; ⁸ &gt; CFU / ml 85.2% none Comparative Example 4 2.2 x 10 &lt; ⁶ &gt; CFU / ml 98.6% Detected Comparative Example 5 2.2 x 10 &lt; ⁶ &gt; CFU / ml 98.6% Detected

As shown in Table 2, it was confirmed that the strain of Comparative Example 3 increased the number of bacteria through the non-aerobic fermentation broth, but the rate of decrease of ammonia was not so high compared to the number of increased bacteria. In Comparative Example 4 (using one fermenter) In the case of 5 (using 3 fermentation tanks), the microorganism activity in the fermentation tank is maintained, but when the fermentation process has been performed in a fermenter for a long time, it is confirmed that harmful microorganisms (E. coli, Salmonella) On the other hand, in Example 4 of the present invention, harmful microorganisms were not detected, and odor reduction ability was excellent. Thus, it was confirmed that the present invention can be used as a desired circulation apparatus.

Thus, the fermentation broth produced by the massive growth of nitrifying bacteria microorganisms is continuously circulated by the slurry pits of the housing, so that the useful microorganisms in the housing slurry pits are ignited, and the odor of the manure generated in the housing is effectively reduced, It is also confirmed that the animal husbandry environment is greatly improved due to the suppression of the proliferation. Also, as the animal manure is circulated for more than 120 days, the odor of the animal manure is increased without the addition of the usual disinfectant or antibiotics without proliferation of harmful microorganisms It can be removed or reduced more quickly and economically.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined by the appended claims. It will be possible. The scope of the present invention is defined by the appended claims, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

10: Housing slurry feet
20: Solid-liquid separator
30: Livestock manure transfer pipe
100: Water tank
200: aerobic fermentation broth
220, 230, 240, 250: Fermenter
260: settling tank
270: active water tank
280: Microorganism incubator
300: fermentation liquid supply pipe
400: Circulating storage
Institution name: Korea Biotechnology Research Institute
Accession number: KCTC13552BP
Checked on: 20180620

<110> LBio Technology Co., Ltd <120> A preparation for fermented liquid fertilizer with          effect reducing odor of livestock waste, and fermented liquid          fertilizer device for reducing odor of livestock waste using the          same <130> SP20181017KR <160> 1 <170> KoPatentin 3.0 <210> 1 <211> 1388 <212> DNA <213> Bacillus sp. <400> 1 tgcagtcgag cgaatctgag ggagcttgct cccaaagatt agcggcggac gggtgagtaa 60 cacgtgggca acctgcctgt aagactggga taactccggg aaaccggggc taataccgga 120 taatatctat ttatacatat aattagattg aaagatggtt ctgctatcac ttacagatgg 180 gcccgcggcg cattagctag ttggtgaggt aacggctcac caaggcgacg atgcgtagcc 240 acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtaggg aatcttccgc aatggacgaa agtctgacgg agcaacgccg cgtgagtgat 360 gaaggttttc ggatcgtaaa actctgttgt tagggaagaa caagtatcgg agtaactgcc 420 ggtaccttga cggtacctaa ccagaaagcc acggctaact acgtgccagc agccgcggta 480 atacgtaggt ggcaagcgtt gtccggaatt attgggcgta aagcgcgcgc aggcggttcc 540 ttaagtctga tgtgaaagcc cacggctcaa ccgtggaggg tcattggaaa ctggggaact 600 tgagtgcaga agaggaaagt ggaattccaa gtgtagcggt gaaatgcgta gagatttgga 660 ggaacaccag tggcgaaggc gactttctgg tctgtaactg acgctgaggc gcgaaagcgt 720 ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg 780 ttagagggtt tccgcccttt agtgctgcag caaacgcatt aagcactccg cctggggagt 840 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg 900 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctc tgacaatcct 960 agagatagga ctttcccctt cgggggacag agtgacaggt ggtgcatggt tgtcgtcagc 1020 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc 1080 agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg 1140 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggatggtaca 1200 aagggctgca agaccgcgag gtttagccaa tcccataaaa ccattctcag ttcggattgt 1260 aggctgcaac tcgcctacat gaagccggaa tcgctagtaa tcgcggatca gcatgccgcg 1320 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1380 cgaagtcg 1388

Claims (5)

A raw water tank 100 for feeding 2 to 5% by weight of livestock manure discharged from the housing slurry pit 10, a fermentation broth preparation preparation and a chelated phyllosilicate, which is a carbon source, based on the weight of the preparation;
An aerobic fermentation liquid non-aerobic tank 200 for fermenting an animal manure mixed with the preparation for fermentation non-production supplied from the raw water tank 100 to produce an aerobic fermentation liquid ratio; And
And a fermentation liquid supply pipe (300) for supplying the aerobic fermentation liquid ratio of the aerobic fermentation liquid non-aerobic tank (200) to the housing slurry pit (10)
The aerobic fermentation broth supplied to the housing slurry pit 10 is mixed with the livestock manure to be supplied to the housing slurry pit 100 through the raw water tank 100, the aerobic fermentation liquid non- 10, and is repeatedly circulated,
The preparation for fermentation broth preparation for use in the raw water tank 100 is composed of a new microorganism Bacillus kochii 4584 strain (KCTC 13552BP) having a nucleotide sequence represented by SEQ ID NO: 1 and having an odor reducing effect on animal manure Characterized in that it comprises a cell or a culture thereof,
Fermentation liquid non - circulation device for livestock manure odor reduction.
The method according to claim 1,
The preparation is a nitrifying bacteria useful microorganism, such as Bacillus psychrodurans , Staphylococcus pasteuri , Bacillus velezensis , Geobacillus stearothermophilus , And a bacterium belonging to the genus Bacillus ginsengihumi , wherein the bacterium comprises a culture of the above-mentioned mixed strain as an active ingredient,
Fermentation liquid non - circulation device for livestock manure odor reduction.
3. The method of claim 2,
Examples of the nitrifying bacteria useful microorganisms include Lactobacillus casei , bacillus methylotrophicus , Lactobacillus parafarraginis , Bacillus subtilis , Acetobacter spp. lovaniensis , and bacillus amyloliquefaciens . The present invention also relates to a method for producing the above-
Fermentation liquid non - circulation device for livestock manure odor reduction.
delete The method according to claim 1,
The aerobic fermentation liquid non-aerobic tank 200 is composed of four fermentation tanks 220, 230, 240 and 250, a settling tank 260 and an active water tank 270.
Fermentation liquid non - circulation device for livestock manure odor reduction.

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