KR102235297B1 - Formulation of compost decomposition using microbial agent and Fertilizer manufactured by using thereof and livestock excrement - Google Patents

Abstract

The present invention relates to a compost decomposition agent using useful microorganisms and a compost prepared by using the same and livestock solids. A method for preparing a compost decomposition agent of the present invention comprises: a first step of preparing a fermented product by adding and mixing a microbial culture medium containing useful microorganisms having thermophilicity, molasses, and sodium chloride in a moisture control agent, followed by fermentation; and a second step of drying the fermented product to prepare a compost decomposition agent having a moisture content of 16 to 20% by weight. The present invention provides a compost decomposition agent and the compost prepared by using the same and livestock solids in which livestock solids are fermented faster at high temperatures so as to immediately compost the same without odor.

Description

{Formulation of compost decomposition using microbial agent and Fertilizer manufactured by using thereof and livestock excrement}

The present invention relates to a composting agent for compost using useful microorganisms and compost manufactured using livestock solids, and more particularly, to ferment livestock solids and livestock solids more quickly at high temperatures so that they can be composted immediately without odor. It relates to compost prepared by using the composting agent and livestock solids.

The annual production of livestock manure is estimated to be 46.99 million tons as of 2016. Specifically, 4 to 5 kg of pig manure and urine are released per day per normal finishing pig. 40kg~50kg per day / 1.2kg~1.5kg per day per chicken / 1.5~2.3kg per day per duck. In other words, as livestock manure of tens of thousands of dollars per day is generated every day in livestock farms where they are raised, the treatment of such livestock manure and urine is a very big problem. In particular, pig manure and urine have the largest amount of flotation among livestock manure, so there is no suitable purification method until now. Currently, these farms are treating through several stages of sedimentation tanks, but they are not suitable for large-scale treatment. In many cases, it is diluted with water and discharged to general rivers, and river pollution by these is pointed out as a big problem.

Accordingly, in order to solve environmental pollution caused by livestock manure mass-produced in livestock farms, recently livestock manure and rice husk, which functions as a moisture control agent, are mixed and stacked, and then taken out to a compost factory for processing. have.

However, in the case of manufacturing compost using livestock manure, rice husk, and sawdust, many problems arise in terms of productiveness. In other words, if livestock manure mass-produced in livestock farms is separately collected and treated, and then left unattended and stacked, fermentation does not take place. Several problems arise, such as occurring as a problem.

Thus, recently, until the production of such high-quality compost compost, sawdust or rice husk is used, but the method of fermentation by mixing while spraying the utilized liquid microorganisms is applied, but this is because the moisture content is too high. There is a problem.

Accordingly, most livestock farms use unripe livestock manure compost that has not been fully ripened for easy and quick treatment of excess livestock manure, or take it out to livestock manure organic fertilizer factories. Root rot or pestilence of crops is caused, and in particular, there is a problem that the crops are killed or disturbed by the gas generated during the fermentation process of unripe compost.

In addition, the government's policy has been revised to improve the reality that people suffer from environmental problems every year, such as disease problems and green algae problems caused by excessive fertilizer by flowing into rivers during the rainy season. In other words, in March 2020, it was legally enforced in March 2020 to remove livestock solids generated from breeding such as chickens, ducks, pigs, cattle, goats, etc. in livestock breeding after fully ripening from each farm. It is urgent to develop a composting agent utilizing useful microorganisms that can be fermented and decomposed in a short time and composted immediately.

1. Republic of Korea Patent Registration No. 10-0945629'Bulky livestock manure decomposition agent fermented with beneficial microorganisms and its manufacturing method' 2. Republic of Korea Patent Laid-Open Patent No. 10-2017-0073157'Bardwood compost with reduced odor using microbial agents and its manufacturing method'

It is an object of the present invention to accumulate various livestock solids generated in each farm every day by adjusting the moisture content while mixing in an appropriate ratio and accumulating the weed grass seeds due to the rapid fermentation in nature and high heat generation. It is to provide a homework for compost that is easily applied for compost use because of the disappearance.

In addition, we have developed a composting agent for composting that can be composted immediately because the gas generation rate is achieved quickly, and through this, it is possible to adjust the moisture content and remove odors, thereby providing compost that can be used immediately in the soil through the natural circulation farming method. have.

In order to achieve the above object, the method of preparing a composting agent for composting of the present invention is a first method of preparing a fermented product by fermenting after mixing a microbial culture solution containing useful microorganisms having thermophilic properties, molasses, and sodium chloride in a moisture control agent. And a second step of drying the fermented product to prepare a composting agent having a moisture content of 16 to 20% by weight.

In addition, when preparing the fermented product of the first step, the moisture control agent is characterized in that it is made of at least one of bulgogi husk or Albasia wood chips that can be crushed by applying pressure to rice husk and impregnated with a solution.

In addition, when preparing the fermented product of the first step, the useful microorganism having thermophilicity is characterized by having a viability at 70 to 100°C, and more preferably, it is characterized in that it is Bacillus kochii 4584 (KCTC 13552BP). .

In addition, when preparing the fermented product of the first step, 200 to 300 ml of the microorganism culture solution, 1 to 3 g of molasses, and 0.1 to 0.5 g of sodium chloride were added and mixed per 1 kg of the moisture control agent, and then 48 to 48 at 25 to 42°C. It is characterized by fermentation for 72 hours.

The compost according to the present invention is characterized in that it is prepared by putting the composting agent for compost prepared through the above manufacturing method into the livestock solids, stirring, and then fermenting after-fermenting at 40 to 75°C for 10 to 45 days.

The technical problems to be solved by the present invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned will be clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. I will be able to.

According to the present invention, a composting agent for composting is provided that allows thermophilic useful microorganisms to survive even with heat generated during fermentation of livestock solids, through which high temperature (70°C or higher) is generated during fermentation of livestock solids, resulting in the extinction of weed grass seeds. In addition, it is possible to completely ripen livestock solids more quickly, so it is possible to provide compost that can be used directly in the soil without odor.

1 is a view showing a manufacturing process chart of compost according to Example 1 of the present invention.
2 is a photograph showing the survival rate at high temperature (90° C., 100° C.) for Bacillus kochii 4584 (KCTC 13552BP), which is one of the useful microorganisms to confirm thermophilicity (test group: after inoculation of useful microorganisms Cultured, negative control: cultured without inoculation of useful microorganisms).
3 is a photograph showing the survival rate at high temperature (90° C., 100° C.) targeting Bacillus methylotrophicus , one of the useful microorganisms, to confirm thermophilicity (test group: inoculated with useful microorganisms and then cultured, negative control group: useful microorganisms Cultured without inoculation).
4 is a photograph (comparative group) showing the survival rate at high temperature (100° C.) targeting Bacillus subtilis (left) and Bacillus subterraneus (right), which are one of the useful microorganisms as homologous strains of FIGS. 2 and 3.
5 is a photograph (comparative group) showing the survival rate at high temperature (100° C.) targeting Bacillus firmus (left) and Bacillus infantis (right), which are one of the useful microorganisms as the homologous strains of FIGS. 2 and 3.

Hereinafter, preferred embodiments and experimental examples of the present invention will be described in detail with reference to the accompanying drawings, and detailed descriptions of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

The present invention relates to a method of manufacturing a composting agent for compost using useful microorganisms and a method of manufacturing compost, which will be described in detail as follows.

<The manufacturing process of compost using the composting agent for compost using the useful microorganism of the present invention>

1. The first process: fermentation production (S10)

In this process, a microbial culture solution containing useful microorganisms having thermophilic properties, molasses, and sodium chloride are added to the moisture control agent, mixed, and fermented to prepare a fermented product.

In other words, in order to maximize composting of livestock solids, the efficacy of the microbial culture medium is very important.

In other words, in order to maximize the decomposition efficiency of livestock solids of the useful microorganism culture medium, a material that can maximize the growth of useful microorganisms is required, and at this time, a moisture control agent is applied in this process.

At this time, the moisture control agent means that the moisture of the livestock meal or the microbial culture medium can be adjusted to facilitate fast fermentation, and more preferably, any one or more of bulgogi or Albasia wood chips is applied. good.

Here, the Albasia wood chip is one of the sawdust that is commonly used as a mushroom medium, and it is known that the moisture absorption rate is three times or more higher than that of the existing oak sawdust.

In addition, the expanded rice hull is prepared in a state in which solution impregnation is possible due to finely chopped rice husk by applying pressure to rice husk in a state in which solution impregnation is not possible, and will be described in detail as follows.

Rice hull refers to the hull of rice, and it supplies rice hull for the purpose of supplying oxygen to aerobic bacteria active in livestock manure in order to make livestock manure mature. In other words, rice husk is characterized by a breathable medium, so it is bulky compared to its mass, and there is a large space inside the material, so oxygen supply is easy. However, since the surface of rice husk is coated with oily ingredients, it cannot absorb moisture well, so if it is mixed with livestock manure and left unattended, it has problems such as rotting problems, resulting in an odor and becoming a breeding ground for flies and mosquitoes.

Therefore, in the present process, the rice husk is not used as it is, but it is prepared in a state in which the rice hull can be impregnated with a solution by applying a certain pressure to the rice hull and applied as a material for a moisture control agent.

As a more specific example, in order to convert the rice husk itself into a state in which solution impregnation is possible, the rice husk is put into a high-pressure screw and is divided into finely by rubbing the rice husk through spatial friction generated by applying a certain pressure. At this time, the particle size is 2 It is about ~5mm, and if the particle size is less than 2mm at this time, the particle is too small to supply oxygen because there is little internal space of the material. During the fermentation process, there is a concern that fermentation with livestock solids may not be performed well, and if it exceeds 5 mm, the particle size is large, and solution impregnation is still not easy.

The Albasia wood chip is also characterized in that its particle size is about 2 to 5 mm, and the reason is similar to that of the expanded chaff.

In the moisture control agent prepared in this way, in order to decompose livestock meal in a short time and to survive high heat, a microbial culture solution containing useful microorganisms having thermophilic properties and molasses and sodium chloride that can promote the growth of microorganisms are added together. After mixing, fermentation is performed to prepare a fermented product.

In other words, when the moisture control agent and microorganisms are simply mixed and applied to livestock solids, the microorganisms are not properly effective, and at the same time, corruption occurs. Thus, when the moisture control agent and microorganisms are first mixed and fermented for a certain period of time to produce a fermented product as in this process, the livestock solids can be decomposed for a short period of time.

At this time, as the applicable useful microorganisms, all useful microorganisms having thermophilicity can be applied, and preferably, the useful microorganisms having thermophilicity have viability at 70 to 100°C, and more preferably, the present inventors It is best to apply Bacillus kochii 4584 (KCTC 13552BP), which was deposited and received by Korea Research Institute of Bioscience and Biotechnology on June 20, 2018.

In addition, the thermophilic useful microorganism Bacillus methylotrophicus (Bacillus methylotrophicus), Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) any one or more strains further included, or the thermophilic useful microorganism Paenivacillus bar Sino norbornene sheath (Paenibacillus barcinonensis), Bacillus Maris flaviviruses (Bacillus marisflavi), Bacillus piece nipo miss (Bacillus licheniformis), Bacillus Pseudomonas mikoyi des (Bacillus pseudomycoides), Bacillus neal soniyi (Bacillus nealsonii), room melriyi Bacillus star Becky time ( Rummeliibacillus stabekisii ), Bacillus Bacillus subtilis , Bacillus velezensis , Bacillus toyonensis , Pichia manshurica , Geobacillus stearothermophilus , Cyclobacillus chic Psychrobacillus psychrodurans , Bacillus Infantis ( Bacillus infantis ) It is best to be configured to further include a general microorganism consisting of any one or more, at this time, the strains are the best that all exist in the same amount.

In other words, the microbial culture medium is characterized by containing useful microorganisms having thermophilic properties that survive at 70 to 100°C. This is when the temperature of the fermentation decomposition product temporarily reaches 70°C or higher in the process of decomposing the following livestock solids by post-ripening fermentation. In order to survive the fermentation and decomposition process, it is very important to apply a thermophilic strain. In particular, it is very important to apply a thermophilic strain. It can also be used immediately.

Accordingly, it is best to include useful microorganisms having thermophilicity in the microorganism culture solution of the present invention, but also include general microorganisms that do not have thermophilicity.

That is, the general microorganisms are strains extracted from conventional livestock manure, and have a very high resolution of livestock solids, and they survive up to 60°C during the fermentation after fermentation after the following livestock manure, and show the resolution of livestock solids up to 60°C. Then, when the temperature exceeds 60° C., the useful microorganisms having thermophilic properties act as a decomposition promoting role. When the temperature exceeds 75° C., the following composting is completed in a short time, and compost use becomes immediately possible.

Herein, the culture medium may mean all substances contained in the medium in which the strain is cultivated, for example, it may mean a substance containing metabolites or secretions that are the result of culturing the strain, or a lysate thereof, and the strain itself is also included in the culture. Can be. In addition, the culture medium may be cultured according to a method commonly used in the art.

As the medium, a conventional medium may be used, and microbial strains containing the thermophilic useful microorganisms are cultured at 20 to 35°C for 92 to 240 hours to obtain a microorganism culture solution having a concentration of ^{1×10 7 CFU/ml or more.} It is characterized in that it is prepared, and more preferably, after inoculating all of the microbial strains in the same amount in the medium, an aeration amount of 0.05 to 0.4 vvm (vol/vol/min) and a stirring speed of 100 for 100 to 120 hours at 20 to 35°C. To 200 rpm (rotary/min), internal pressure of 0.1 to 1 kgf/cm2, and the pH of the medium is most preferably 6.0 to 9.0.

The microbial culture broth is characterized in that it is prepared by fermentation after mixing and mixing 200 to 300 ㎖ per 1 kg of the moisture control agent.If less than 200 ㎖ per 1 kg of the moisture control agent is added, the content is too small to prevent proper fermentation. If it exceeds 300 ml, it is not suitable for composting due to over-fermentation. In addition, the molasses is characterized by using 1 ~ 3g per 1 kg of the moisture control agent, the sodium chloride is 0.1 ~ 0.5g each, this is to promote the growth of useful microorganisms, molasses contains less than 1 g or sodium chloride is 0.1 If it contains less than g, it is difficult to obtain the increased resolution of livestock solids because the growth promoting ability of useful microorganisms is not exhibited.If the molasses content exceeds 3g or sodium chloride content exceeds 0.5g, it is rather useful depending on the excess. There is a fear that it will interfere with the growth of microorganisms.

In addition, a specific fermentation condition applied in this process is characterized in that it is performed for 48 to 72 hours at 25 to 42°C, and the fermentation condition is a condition to maximize the effect of microorganisms, such as maintaining thermophilicity of useful microorganisms. Thus, when the fermentation conditions are out of the above, the fermentation conditions do not match and the thermophilicity of the microorganisms is rather deteriorated.

2. Second process: manufacturing composting material (S20)

In this process, the fermented product is dried to prepare a composting agent having a moisture content of 16 to 20% by weight.

In other words, the fermented product itself contains too much moisture, making it difficult to maintain a human state, and as it continues to be activated, the number of bacteria decreases, and the effect of the microorganism itself decreases. This will not work properly.

Thus, the fermented product is gradually dried for 40 to 50 hours at 20 to 25°C to have a moisture content of 16 to 20% by weight in order to maintain a human state without deterioration of the microorganisms present in the fermented product. It can be applied immediately as a composting homework of the invention.

In other words, if the moisture content is less than 16% by weight, the content is too small, making it difficult for the microorganisms to survive, or there is a risk that the microorganisms may be destroyed due to the excessive control of the moisture content, and if it exceeds 20% by weight, it is still It is not easy to maintain the human state as there is a concern about the deterioration of microorganisms.

3. 3rd process: compost manufacturing (S30)

The prepared composting agent is characterized in that it is mixed with livestock solids and then fermented after ripening to prepare final compost.

At this time, it is characterized in that it is manufactured by putting 20 to 200 kg of the composting agent per ton of livestock solid content and then fermenting after 10 to 45 days at 40 to 75°C. In order to shorten the time of fermentation after fermentation, up to 200 kg is applied. It is possible, and if it is contained in more than 200kg, the increased efficacy according to the excess content does not appear. In addition, when the content of the composting agent for composting is less than 20 kg, the content is too small, which makes it difficult to quickly compost, which is the object of the present invention.

In addition, in the case of fermentation below 40℃ under the above-mentioned post-fermentation conditions, the viability of microorganisms remaining in the composting agent decreases, so that fermentation is not properly performed. If fermentation exceeds 75℃, it is generated from the livestock solids itself. The odor makes it unsuitable for composting.

This process of fermentation after ripening takes place for 10 to 15 days or up to 45 days depending on the livestock solids, and after that time, it can be applied as compost immediately. It is possible to shorten the manufacturing time.

Here, as the livestock solid content, manure from mammals such as cattle, pigs, sheep, and goats and poultry such as pheasants, chickens, ducks, and turkeys may be used, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but these Examples and Experimental Examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.

<Example 1> Preparation of compost 1 of the present invention

After transferring the commercially purchased rice husk to the inside of the high-pressure screw through a conveyor, a high pressure of 10 kgf/cm2 was applied in the screw state to produce friction between the rice husks and crushed finely to produce a solution-impregnable expanded chaff. At this time, as high heat of 280° C. or higher was generated inside the high-pressure screw, high-pressure steam was sprayed to cool the heat to prevent heat generation, and the expanded chaff was prepared.

To 1 kg of expanded rice bran having a particle size of 5 mm thus prepared, 300 ml of a thermophilic useful microorganism culture solution, 2 g of molasses, and 0.3 g of sodium chloride were added, mixed, and fermented at 25° C. for 48 hours to prepare a fermented product.

At this time, the thermophilic useful microorganism culture medium was prepared in the form of a culture medium having a concentration of ^{1×10 7} CFU/ml by incubating the useful microorganisms of Bacillus kochii 4584 (KCTC 13552BP) at 30° C. for about 240 hours. Was used.

The fermented product thus prepared was gradually dried at 20° C. for 48 hours to prepare a composting agent for compost having a moisture content of 20% by weight, and then stored for 30 days.

Then, 200kg of the composting agent and 1 ton of livestock solids (beef meal) were mixed and then fermented at 40° C. for 15 days to prepare compost 1 according to the present invention.

<Example 2> Preparation of compost 2 of the present invention

Prepared in the same manner as in Example 1, but the useful microbial culture medium having thermophilicity is Bacillus kochii 4584 (KCTC 13552BP), Bacillus horikoshii , Bacillus methylotrophicus , After mixing the useful microorganisms of Bacillus amyloliquefaciens in the same amount, incubating at 30° C. for about 240 hours, and using the prepared in the form of a culture medium having a concentration of ^{1×10 7 CFU/ml, the present inventor's compost 2} Was prepared.

<Example 3> Preparation of compost 3 of the present invention

Prepared in the same manner as in Example 1, but applying Albasia wood chips instead of the bulging chaff, and as the useful microorganisms, Bacillus kochii 4584 (KCTC 13552BP), Bacillus horikoshii , Bacillus methyl Bacillus methylotrophicus , Bacillus amyloliquefaciens , Paenibacillus bacinonensis , Bacillus marisflavi , Bacillus marisflavi, Bacillus lichenifomis pseudo mikoyi death (Bacillus pseudomycoides), neal soniyi Bacillus (Bacillus nealsonii), Room bacillus melriyi Becky star City (Rummeliibacillus stabekisii), Bacillus Bacillus subtilis , Bacillus velezensis , Bacillus toyonensis , Pichia manshurica , Geobacillus stearothermophilus , Cyclobacillus chic Psychrobacillus psychrodurans , Bacillus Compost 3 of the present invention was prepared by applying a microorganism culture solution in which Bacillus infantis was mixed in the same amount.

<Comparative Example 1> Preparation of compost 4

Compost 4 was prepared in the same manner as in Example 1, but by applying 1 ton of livestock manure mixed with livestock solids and urine instead of the livestock solids.

<Comparative Example 2> Preparation of compost 5

Compost 5 was prepared in the same manner as in Example 1, but using a useful microorganism preparation in which Bacillus subtilis and Bacillus infantis were equally mixed as the useful microorganisms.

<Comparative Example 3> Preparation of compost 6

Compost 6 was prepared in the same manner as in Example 1, but by applying the rice husk as it was excluding the expanded rice husk manufacturing process.

<Comparative Example 4> Preparation of compost 7

After mixing 100 kg of expanded rice husk of Example 1 with 100 kg of the useful microorganism culture solution, 5 g of molasses, and 0.1 g of sodium chloride, after 30 days elapsed, 1 ton of livestock solid content was mixed and fermented after fermentation at 40°C for 15 days to compost 7 Was prepared.

<Comparative Example 5> Preparation of compost 8

It was prepared in the same manner as in Example 1, but compost 8 was prepared by applying rice bran instead of the rice husk.

<Experimental Example 1> Confirmation of thermophilicity of useful microorganisms by homogeneous strain

1. Experimental method

Bacillus kochii 4584 (KCTC 13552BP), Bacillus horikoshii strain, Bacillus amyloliquefaciens strain, Bacillus methylotrophicus strain, Bacillus subtilis strain, Bacillus subterraneus strain, Bacillus firmus strain, and Bacillus infantis strain total 8 strains (NB) for a total of 8 strains. Each inoculated to and cultured with shaking at 35° C. for 16 hours, and the cultured strain cultures were reacted at 25° C. for 1 hour. After 1 ml of the culture solution reacted for 1 hour was added to each of the two tubes, one tube of the two tubes to which 1 ml of the culture solution was added was reacted at 25°C for 3 hours, and the other tube was reacted at 90°C for 3 hours. .

Cultures of tubes reacted at 25°C and 90°C for 3 hours were plated on Nutrient medium agar plate, respectively, and then incubated at 35°C for 16 hours. The cultured Nutrient medium agar plate was photographed to measure CFU (Colony Forming Unit).

In the same manner as above, the test was also conducted under the conditions of 25°C, 75°C, 90°C, and 100°C.

In the test of the negative control group, the test was also conducted under the conditions of 25°C and 100°C in the same manner as above without inoculating the bacteria.

2. Experiment result

The experimental results were shown in Table 1 and FIGS. 2 and 3 below.

Survival rate at 25°C Survival rate at 75℃ Bacillus kochii 100% 100% Bacillus horikoshii strain 100% 100% Bacillus amyloliquefaciens strain 100% 100% Bacillus methylotrophicus strain 100% 100% Bacillus subtilis strain 100% 10% Bacillus subterraneus strain 100% 10% Bacillus firmus strain 100% 0% Bacillus infantis strain 100% 0%

As shown above in Table 1. All kochii Bacillus strain, strain Bacillus horikoshii, Bacillus amyloliquefaciens strain, Bacillus strain methylotrophicus 25 ℃ And it showed 100% survival rate at 75 ℃, as shown in Figs. 2 and 3, Bacillus kochii microorganisms exhibited a survival rate of 90% at 90 ℃, and 80% at 100 ℃, Bacillus methylotrophicus microorganisms were 90 ℃ At 90% survival rate and 70% survival rate at 100 ℃ bar, when the fermentation decomposition process at a high temperature of 70 ℃ or higher as in the present invention is applied, Bacillus kochii strain, Bacillus horikoshii strain, Bacillus amyloliquefaciens strain, which has thermophilic properties, Bacillus methylotrophicus strains were found to be suitable.

On the other hand, as shown in Table 1, Figs. 4 and 5, Bacillus subtilis and Bacillus subterraneus showed 100% survival rate at 25℃, but 10% survival rate at 75℃ and 5% survival rate at 100℃. Bacillus firmus and Bacillus infantis also showed 100% survival rate at 25℃. It can be seen that none of the Bacillus strains exhibited thermophilicity of 70° C. or more, as a bar showing a survival rate of 0% at 75°C and 0% at 100°C.

<Experimental Example 2> Compost Composting Effect

1. Experimental method

The compost saturation effect was confirmed for the composts of Examples 1 and 2, and the composts of Comparative Examples 1 to 5, and at this time, the moisture content and the _{change in hydrogen sulfide (H 2} S) concentration were measured as test items, at this time, The concentration of the test gas was measured by a gas detection tube (formerly KS I 2218:2009). In addition, 200 kg of rice husk was added to 1 ton of livestock solids (beef meal) and post-fermented at 25°C for 15 days.

As a result of the above experiment, it was shown in Table 2 below.

Moisture moisture content H ₂ S gas reduction rate (%) No treatment 43.9% - Compost 1 of Example 1 20% 80% Compost 2 of Example 2 18% 85% Compost 3 of Example 3 16% 95% Compost 4 of Comparative Example 1 70% - Compost 5 of Comparative Example 2 40% - Compost 6 of Comparative Example 3 38% 5% Compost 7 of Comparative Example 4 40% - Compost 8 of Comparative Example 5 35% 10%

As shown in Table 2 above, when comparing the moisture content, the untreated section showed 43.9%, whereas after 15 days, the compost 1 of Example 1 was 20%, the compost 2 of Example 2 was 18%, and the compost 3 of Example 3 was Was found to be as low as 16%, and it was confirmed that the composting agent of the present invention accelerated composting and stabilized rapidly. In particular, in the case of Example 3, useful microorganisms having thermophilic properties and general microorganisms were applied together, and general microorganisms having a high resolution of livestock solids up to 60°C during fermentation after fermentation showed the resolution, and then the temperature gradually increased. When the temperature exceeded 60° C., the useful microorganisms having thermophilic properties acted and exhibited the effect of promoting decomposition, and it was confirmed that the hydrogen sulfide reduction rate was the lowest.

On the other hand, the compost 4 of Comparative Example 1 is a mixture of livestock meal and urine, and the state before composting itself has a high moisture content. I did.

Compost 5 of Comparative Example 2 was obtained by applying other strains with low thermophilicity, and it was confirmed that the microorganisms were almost killed due to the high heat generated at the time of composting, which was also difficult to compost. Compost 6 of Comparative Example 3 was confirmed that the rice husk was applied as it was, and thus fermentation was not properly performed because sufficient microbial impregnation was not performed, so it was also confirmed that more time was required to compost.

Compost 7 of Comparative Example 4 is a mixed state of sprouting chaff and useful microorganism culture medium, and thus it is not maintained in a human state, so when the strain is continuously activated during storage for 30 days, when it is fertilized in a membrane, useful microorganisms cannot play a role of repellency. It was also confirmed that it was difficult to compost.

Compost 8 of Comparative Example 5 was found to have a possibility of composting compared to other Comparative Examples, but it was confirmed that more time was required for composting, since it was insufficient for composting in a short period of time.

The hydrogen sulfide reduction rate also showed similar results to the moisture content, and it was confirmed that the fertilizers 1 to 3 of Examples 1 to 3 showed high odor reducing ability in a short period of time.

<Experimental Example 3> Crop growth effect

1. Experimental method

To check the crop growth effect for the compost 1 of Example 1, the compost 2 of Example 2, and the compost 3 of Example 3, 25 ± 2 ℃ (weekly), humidity of 62 ± 5%, 15 hours of light Under the above conditions, 100 ml of water was supplied to each pot at intervals of 7 days, and the culture soil and compost of Example 1 were stirred and fertilized at 50 g of each pot.

In order to investigate the height of the above-ground part of the plant, on the 30th day after the compost treatment, the height of the above-ground part of the largest 10 specimens in each extracted sample was measured in units of 0.01cm using a VERNIER CALIPER. I did.

As for the control, grass seeds were grown as described above, but grass seeds were grown after processing compost for general gardening (company h).

2. Experiment results,

As a result of the above experiment, it was shown in Table 3 below.

Control Compost 1 of Example 1 Compost 2 of Example 2 Compost 3 of Example 3 Grass seeds 4.42±0.51 8.95±0.62 9.02±0.33 9.59±0.25

As shown in Table 3, it was confirmed that the treatment groups treated with the compost 1 of Example 1, the compost 2 of Example 2, and the compost 3 of Example 3 had remarkably superior effect of plant growth compared to the control. While the weed grass seeds were grown together, it was confirmed that the weed grass seeds did not grow in the treatments treated with the compost 1 of Example 1, the compost 2 of Example 2, and the compost 3 of Example 3.

As described above, in the present invention, it is possible to provide a composting agent for composting in which thermophilic useful microorganisms can survive even heat generated during fermentation of livestock solids.Through this, livestock solids can be completely ripened, so that they can be used directly in the soil without odor. It was confirmed through the above experiments that compost with good can also be provided.

The present invention has been looked at around preferred embodiments, and those of ordinary skill in the technical field to which the present invention pertains can implement the detailed description of the present invention and other types of embodiments within the essential technical scope of the present invention. I will be able to. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Korea Research Institute of Bioscience and Biotechnology KCTC13552BP 20180620

<110> Lbio technology co.,ltd <120> Formulation of compost decomposition using microbial agent and Fertilizer manufactured by using thereof and livestock excremen <130> Lbio-02 <150> KR 10-2019-0132249 <151> 2019-10-23 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1388 <212> DNA <213> bacillus sp. <400> 1 tgcagtcgag cgaatctgag ggagcttgct cccaaagatt agcggcggac gggtgagtaa 60 cacgtgggca acctgcctgt aagactggga taactccggg aaaccggggc taataccgga 120 taatatctat ttatacatat aattagattg aaagatggtt ctgctatcac ttacagatgg 180 gcccgcggcg cattagctag ttggtgaggt aacggctcac caaggcgacg atgcgtagcc 240 acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtaggg aatcttccgc aatggacgaa agtctgacgg agcaacgccg cgtgagtgat 360 gaaggttttc ggatcgtaaa actctgttgt tagggaagaa caagtatcgg agtaactgcc 420 ggtaccttga cggtacctaa ccagaaagcc acggctaact acgtgccagc agccgcggta 480 atacgtaggt ggcaagcgtt gtccggaatt attgggcgta aagcgcgcgc aggcggttcc 540 ttaagtctga tgtgaaagcc cacggctcaa ccgtggaggg tcattggaaa ctggggaact 600 tgagtgcaga agaggaaagt ggaattccaa gtgtagcggt gaaatgcgta gagatttgga 660 ggaacaccag tggcgaaggc gactttctgg tctgtaactg acgctgaggc gcgaaagcgt 720 ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg 780 ttagagggtt tccgcccttt agtgctgcag caaacgcatt aagcactccg cctggggagt 840 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg 900 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctc tgacaatcct 960 agagatagga ctttcccctt cgggggacag agtgacaggt ggtgcatggt tgtcgtcagc 1020 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc 1080 agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg 1140 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggatggtaca 1200 aagggctgca agaccgcgag gtttagccaa tcccataaaa ccattctcag ttcggattgt 1260 aggctgcaac tcgcctacat gaagccggaa tcgctagtaa tcgcggatca gcatgccgcg 1320 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1380 cgaagtcg 1388

Claims (7)

It is possible to impregnate the solution by applying pressure to the rice hull, and containing thermophilic useful microorganisms having viability at 70 ~ 100℃ in a moisture control agent consisting of one or more of expanded rice hull or Albasia wood chips having a particle size of 2 ~ 5mm. Microbial culture broth, molasses, sodium chloride are added, mixed, and fermented to prepare a fermented product, but 200 to 300 ml of the microbial culture broth, 1 to 3 g of molasses, and 0.1 to 0.5 g of sodium chloride are added and mixed per 1 kg of the moisture control agent. The first step of fermenting at 25 to 42°C for 48 to 72 hours to prepare a fermented product, and
A second step of drying the fermented product at 20 to 25° C. for 40 to 50 hours to prepare a composting agent for compost having a moisture content of 16 to 20% by weight; including,
Method of manufacturing composting homework.
delete delete The method of claim 1,
The thermophilic useful microorganism is characterized in that Bacillus kochii 4584 (KCTC 13552BP),
Method of manufacturing composting homework.
delete It is prepared by the manufacturing method of any one of claims 1 and 4, and includes thermophilic useful microorganisms having viability at 70 to 100°C, characterized by having a moisture content of 16 to 20% by weight,
Compost homework.
Prepared by adding and stirring the composting agent for compost of Clause 6 to the livestock solid content, but putting 20-200kg of the composting agent for composting per ton of the livestock solid content and stirring, followed by post-fermentation at 40-75°C for 10 to 45 days,
compost.

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