CN105132300A - Method for preparing natural water ecological purification microbial inoculum - Google Patents
Method for preparing natural water ecological purification microbial inoculum Download PDFInfo
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- CN105132300A CN105132300A CN201510640034.1A CN201510640034A CN105132300A CN 105132300 A CN105132300 A CN 105132300A CN 201510640034 A CN201510640034 A CN 201510640034A CN 105132300 A CN105132300 A CN 105132300A
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Abstract
The invention discloses a method for preparing a natural water ecological purification microbial inoculum. The natural water ecological purification microbial inoculum is prepared from saccharomycetes microbial inoculum, lactic acid bacterium microbial inoculum, denitrifying bacterium microbial inoculum, bacillus subtilis microbial inoculum and photosynthetic bacterium microbial inoculum. The natural water ecological purification microbial inoculum obtained with the method can remove eutrophication materials in water effectively and has a certain bottom mud digestion effect. When the natural water ecological purification microbial inoculum is used, oxygen consumption is quite low, water can be cleared, bottom mud can be degraded, and the overall water environment of natural water can be improved.
Description
Technical field
The invention belongs to environmental microorganism engineering field, be specifically related to a kind of preparation method of natural water ecological purification microbial inoculum.
Background technology
The restoration of the ecosystem utilizing environmental microorganism technology to carry out polluted-water at present has become the main flow of water pollution Treatment process development.Its principle of work mainly relies on the natural degradation ability of the microorganism species that can be present in contaminated water body or artificial creation suitable micro-organisms degraded and absorbed condition, general adopts through the microorganism of domestication and cultivation and commercial suitable micro-organisms microbial inoculum with the removal ability of enhancement microbiological flora to pollutent.
Summary of the invention
Object of the present invention provides a kind of preparation method of natural water ecological purification microbial inoculum, and this product is obvious to the Eutrophication materials removal effect existed in water body, and has certain bed mud digestion effect.In application, oxygen-consumption is lower, can clarify water quality, degraded bed mud, has the effect of the overall water quality environment of improvement natural water.The present invention utilizes the feature that the microorganism growth generation is short, can tame, be convenient to cultivation, is absorbed the feature of a large number of nutrients, reach the nutritive substance consuming and pollute in water body, make the object that water quality improves by it in necessary for growth.The multiple degrading enzyme that the symbiosis particularly occurred in its process of growth and mutuality produce, effectively can reduce the accumulation of pollution substance in water surrounding.Utilize simultaneously part bacterium to organic digestion, the accumulation of the water-bed organogenous sediments of natural water can be reduced.A kind of excellent biological products of high effect nontoxic.
The invention provides a kind of natural water ecological purification microbial inoculum, by different combinations, difference in functionality microorganism species can be formed, specific as follows:
A flora is EM (Effectivemicroorganisms) flora, in the present invention, involved EM flora is milk-acid bacteria, and yeast, can produce the various active factor, promote the absorption to biological nutrition material, and the contaminative nutritive substance existed in water body is reduced.
B flora is cellulose degradation flora, the present invention relates to subtilis, yeast, flocculated bacteria.The Mierocrystalline cellulose of difficult degradation and other macromolecular cpd are resolved into micromolecular compound be easily biodegradable, simultaneously also for other microorganism provides nutrition, increase Microflora in environment, strengthen the degradation effect of the nutritive substance to contaminative in natural water.
C flora is nitrate radical degradation flora, and what the present invention relates to is subtilis, and denitrifying bacterium and photosynthetic bacterium reduce and eliminate the accumulation of nitrate radical effectively.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of natural water ecological purification microbial inoculum, formulated by weight by following raw material:
A kind of natural water ecological purification microbial inoculum, by following raw material by weight formulated (preferable range):
A kind of natural water ecological purification microbial inoculum, by following raw material by weight formulated (optimum value):
A preparation method for natural water ecological purification microbial inoculum, its preparation process is:
The preparation method of yeast microbial inoculum: yeast is inoculated in microzyme culture medium and carries out activation culture, the condition of activation culture is: temperature 30 DEG C ~ 32 DEG C, static gas wave refrigerator 24h ~ 48h, the inoculum size of yeast seeds is 1 ~ 2%, obtains the yeast seed liquor of activation;
Yeast seed liquor after above-mentioned activation is inoculated in the fermentor tank being placed with yeast enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: inoculum size is 5-10%, pH value controls 7.0 ~ 7.2, temperature is 30 DEG C ~ 32 DEG C, static fermentation, intermittent stirring is carried out in 1 hour in interval, fermentation 48 ~ 72h, fermentor tank tank pressure 0.02 ~ 0.05 MPa, dry acquisition yeast microbial inoculum after enlarged culturing;
Wherein, microzyme culture medium configures according to following proportioning raw materials: sucrose 50g, K
2hPO
45g, MgSO
42g, (NH
4)
2sO
43g, agar 15g, distilled water 1L.
Yeast enlarged culturing base configures according to following proportioning raw materials: shared by each composition of yeast enlarged culturing base, yeast enlarged culturing base mass percent is: molasses 2% or 1.5% or 1.0%, Rice & peanut milk 0.5% or 0.4% or 0.3%, K
2hPO
40.5%, MgSO
40.2%, (NH
4)
2sO
40.3%, be left for distilled water.
The preparation method of milk-acid bacteria microbial inoculum:
Lactobacillus inoculum is carried out activation culture in lactic acid bacteria culturing medium, and the condition of activation culture is: temperature 30 DEG C ~ 32 DEG C, and 24h ~ 48h is cultivated in 150 ~ 180rpm jolting, and inoculum size is 0.8 ~ 1.5%, obtains the lactobacillus solution of activation;
The above-mentioned lactobacillus solution by activation is inoculated in the fermentor tank being placed with milk-acid bacteria enlarged culturing base and carries out enlarged culturing, enlarged culturing condition: inoculum size is 5-10%, pH value controls 6.8 ~ 7.2, aerobic fermentation, fermentation ventilation rate is 1 ︰ 0.5 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.02 ~ 0.05 MPa, temperature 30 DEG C ± 1 DEG C, cultivate 40h ~ 50h, dry acquisition milk-acid bacteria microbial inoculum after enlarged culturing;
Wherein, lactic acid bacteria culturing medium configures according to following proportioning raw materials: it is peptone 0.5% that each composition of lactic acid bacteria culturing medium accounts for lactic acid bacteria culturing medium mass percent, extractum carnis 0.5%, yeast extract paste 0.5%, glucose 2%, lactose 2%, CaCO
31%, agar 1.5%, toluylene red 0.005%, is left for distilled water;
Milk-acid bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of milk-acid bacteria enlarged culturing base, milk-acid bacteria enlarged culturing base mass percent is: molasses 2% or 1.5% or 1.0%, corn steep liquor 0.5% or 0.4% or 0.3%, CaCO
31%, toluylene red 0.005%, is left for distilled water.
The preparation method of denitrifying bacterium microbial inoculum: denitrifying bacterium is inoculated in denitrifying bacteria substratum and carries out activation culture, the condition of activation culture is: temperature 28 DEG C ~ 32 DEG C, 100 ~ 200rpm jolting, cultivate 24h ~ 48h, inoculum size is 0.8 ~ 1.5%, obtains the denitrifying bacterium seed liquor of activation;
The denitrifying bacteria seed liquor of above-mentioned activation is inoculated in the fermentor tank being placed with denitrifying bacteria enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: by inoculum size 8 ~ 12%, pH controls 7.0 ~ 7.3, aerobic fermentation, fermentation ventilation rate 1 ︰ 0.5 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.02 ~ 0.05 MPa, temperature is 30 DEG C ~ 32 DEG C, cultivate 36 ~ 38h, dry acquisition denitrifying bacterium microbial inoculum after enlarged culturing.
Wherein, denitrifying bacteria substratum configures according to following proportioning raw materials: the mass percent that each composition of denitrifying bacteria substratum accounts for denitrifying bacteria substratum is saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, agar 1.5%, remaining is distilled water.
Denitrifying bacteria enlarged culturing base configures according to following proportioning raw materials: the mass percent of denitrifying bacteria enlarged culturing base shared by each composition of denitrifying bacteria enlarged culturing base is molasses 2% or 1.5% or 1.0%, corn steep liquor 0.5% or 0.4% or 0.3%, saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, is left for distilled water.
The preparation method of bacillus subtilis microbial agent: subtilis is inoculated in bacillus subtilis bacterium culture medium and carries out activation culture, activation culture condition: temperature is 25 ~ 30 DEG C, 100rpm ~ 200rpm vibrates cultivation, time is 20 ~ 24h, inoculum size is 2% ~ 3%, obtains the subtilis seed liquor activated;
The subtilis seed liquor of above-mentioned activation is inoculated in the fermentor tank being provided with subtilis enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: inoculum size is with 3% ~ 10%, pH controls 7.0 ~ 7.3, carry out aerobic fermentation, fermentation ventilation rate fermentation ventilation rate 1 ︰ 0.5 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.02 ~ 0.05 MPa, temperature 30 DEG C ~ 32 DEG C, cultivate 36 ~ 38h, dry acquisition bacillus subtilis microbial agent after enlarged culturing.
Wherein, bacillus subtilis bacterium culture medium configures according to following proportioning raw materials: shared by each composition of bacillus subtilis bacterium culture medium, subtilis substratum mass percent is glucose 2%, peptone 1.5%, sodium-chlor 0.5%, extractum carnis 0.05%, agar 2%, remaining is distilled water.
Subtilis enlarged culturing base configures according to following proportioning raw materials: the mass percent that each composition of subtilis enlarged culturing base accounts for as subtilis enlarged culturing base is: molasses 2% or 1.5% or 1.0%, corn steep liquor 0.5% or 0.4% or 0.3%, peptone 1.5%, sodium-chlor 0.5%, is left for distilled water.
The preparation method of photosynthetic bacterium microbial inoculum: photosynthetic bacterium is inoculated in photosynthetic bacteria culture medium and carries out activation culture, activation culture condition: temperature is 25 ~ 34 DEG C, and 100rpm ~ 200rpm vibrates cultivation, the time is 20 ~ 24h, inoculum size is 3% ~ 5%, obtains the photosynthetic bacterium seed liquor after activating;
The photosynthetic bacterium seed liquor of above-mentioned activation is inoculated in the transparent airtight container being provided with photosynthetic bacterium enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: higher to the inoculum size of photosynthetic bacterium enlarged culturing, the inoculum size of light and bacterium seed liquor is generally 20% ~ 50%, namely the ratio of light and bacterium seed liquor and photosynthetic bacterium enlarged culturing base unit weight is 1:4 ~ 1:1, and should lower than 1:5.PH7.5 ~ 8.5, intensity of illumination: 3000Lx-4000Lx is best, and namely every 25 kilograms of bacterium liquid need carry out illumination with the electricbulb of about 60 watts or sunlight, cultivate 36 ~ 38h, dryly after enlarged culturing obtains photosynthetic bacterium microbial inoculum.
Photosynthetic bacteria culture medium configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacteria culture medium accounts for photosynthetic bacteria culture medium mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, agar 2%, remaining is distilled water.
Photosynthetic bacterium enlarged culturing base configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacterium enlarged culturing base accounts for photosynthetic bacterium enlarged culturing base mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, remaining is distilled water.
By obtained yeast microbial inoculum, milk-acid bacteria microbial inoculum, denitrifying bacterium microbial inoculum, nitrite bacteria microbial inoculum, bacillus subtilis microbial agent and photosynthetic bacterium microbial inoculum, according to the obtained a kind of natural water ecological purification microbial inoculum of weight part mixing of 1 ~ 2:1 ~ 3:3 ~ 4:4 ~ 6:2 ~ 5.
Described subtilis (Bacillussubtilis), derives from Wuhan University's China typical culture collection center, deposit number CCTCCNO:2015468;
Described yeast (saccharomycetes), derives from Wuhan University's China typical culture collection center, deposit number CCTCCNO:2015469;
Described photosynthetic bacterium (photosyntheticbacteria), derives from Wuhan University's China typical culture collection center, deposit number CCTCCNO:2015470;
Described denitrifying bacterium (denitrifyingbacteria), derives from Wuhan University's China typical culture collection center, deposit number CCTCCNO:2015471;
Described milk-acid bacteria (lactobacillus), any commercial above-mentioned bacterial strains all can realize the present invention.
The present invention compared with prior art, has the following advantages:
1. this ecological purification microbial inoculum, adopt extension fermentation culture technology, obtained microbial inoculum purity high miscellaneous bacteria is less, and bacterium coordinated growth is better, and the loss amount of bacterium is little.
2. the phosphorus containg substances such as the organophosphorus that the withered grass spore genus bacillus in this ecological purification microbial inoculum composition can effectively be degraded in water body and bed mud, there is very strong restraining effect to unwanted bacterias such as the vibrios that may exist in water quality, intestinal bacteria and baculoviruss simultaneously, and have positive effect to water body purification.
3. in this ecological purification microbial inoculum, each inoculating proportion is reasonable, the effective viable bacteria content of the granule type composite microbial agent made is high, after using water body, local viable bacteria concentration is high, and between each bacterial classification, there is synergy, can be good at the effect playing microbial inoculum, extraordinary improving effect is played to bed mud and water quality.
4. this ecological purification microbial inoculum, can divide fast after using and be spread in water body, and be uniformly distributed, and directly acts on the contaminative nutritive substance improvement water body environment in water body, prevents bed mud corrupt, suppress the breeding of harmful bacteria in bed mud, available protecting water quality.
5. the yeast in this ecological purification microbial inoculum, denitrifying bacterium, photosynthetic bacterium belong to low oxygen consumption, not oxygen consumption bacterial classification, general seriously polluted water body and water bottom oxygen level all lower, this patent ecological purification microbial inoculum is used not consume water body dissolved oxygen in such a case, and water body environment oxygen level can be made to increase, the existence for bottom hydrocoles provides good environment.
Embodiment
Embodiment 1:
A preparation method for natural water ecological purification microbial inoculum, its preparation process is:
The preparation method of yeast microbial inoculum: yeast is inoculated in microzyme culture medium and carries out activation culture, the condition of activation culture is: temperature 30 DEG C, static gas wave refrigerator 24h, and the inoculum size of yeast seeds is 1%, obtains the yeast seed liquor of activation;
Yeast seed liquor after above-mentioned activation is inoculated in the fermentor tank being placed with yeast enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: inoculum size is 5%, pH value controls 7.0, temperature is 30 DEG C, static fermentation, intermittent stirring is carried out in 1 hour in interval, fermentation 48h, fermentor tank tank pressure 0.02 MPa, dry acquisition yeast microbial inoculum after enlarged culturing;
Wherein, microzyme culture medium configures according to following proportioning raw materials: sucrose 50g, K
2hPO
45g, MgSO
42g, (NH
4)
2sO
43g, agar 15g, distilled water 1L.
Yeast enlarged culturing base configures according to following proportioning raw materials: shared by each composition of yeast enlarged culturing base, yeast enlarged culturing base mass percent is: molasses 2%, Rice & peanut milk 0.5%, K
2hPO
40.5%, MgSO
40.2%, (NH
4)
2sO
40.3%, be left for distilled water.
The preparation method of milk-acid bacteria microbial inoculum:
Lactobacillus inoculum is carried out activation culture in lactic acid bacteria culturing medium, and the condition of activation culture is: temperature 30 DEG C, and 24h is cultivated in 150rpm jolting, and inoculum size is 0.8%, obtains the lactobacillus solution of activation;
The above-mentioned lactobacillus solution by activation is inoculated in the fermentor tank being placed with milk-acid bacteria enlarged culturing base and carries out enlarged culturing, enlarged culturing condition: inoculum size is 5%, pH value controls 6.8, aerobic fermentation, fermentation ventilation rate is 1 ︰ 0.5, fermentor tank tank pressure 0.02 MPa, temperature 29 DEG C, cultivate 40h, dry acquisition milk-acid bacteria microbial inoculum after enlarged culturing;
Wherein, lactic acid bacteria culturing medium configures according to following proportioning raw materials: it is peptone 0.5% that each composition of lactic acid bacteria culturing medium accounts for lactic acid bacteria culturing medium mass percent, extractum carnis 0.5%, yeast extract paste 0.5%, glucose 2%, lactose 2%, CaCO
31%, agar 1.5%, toluylene red 0.005%, is left for distilled water;
Milk-acid bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of milk-acid bacteria enlarged culturing base, milk-acid bacteria enlarged culturing base mass percent is: molasses 2%, corn steep liquor 0.5%, CaCO
31%, toluylene red 0.005%, is left for distilled water.
The preparation method of denitrifying bacterium microbial inoculum: denitrifying bacterium is inoculated in denitrifying bacteria substratum and carries out activation culture, the condition of activation culture is: temperature 28 DEG C, 100rpm jolting, and cultivate 24h, inoculum size is 0.8%, obtains the denitrifying bacterium seed liquor of activation;
The denitrifying bacteria seed liquor of above-mentioned activation is inoculated in the fermentor tank being placed with denitrifying bacteria enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: by inoculum size 8%, pH controls 7.0, aerobic fermentation, fermentation ventilation rate 1 ︰ 0.5, fermentor tank tank pressure 0.02 MPa, temperature is 30 DEG C, cultivate 36h, dry acquisition denitrifying bacterium microbial inoculum after enlarged culturing.
Wherein, denitrifying bacteria substratum configures according to following proportioning raw materials: the mass percent that each composition of denitrifying bacteria substratum accounts for denitrifying bacteria substratum is saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, agar 1.5%, remaining is distilled water.
Denitrifying bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of denitrifying bacteria enlarged culturing base, the mass percent of denitrifying bacteria enlarged culturing base is molasses 2%, corn steep liquor 0.5%, saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, is left for distilled water.
The preparation method of bacillus subtilis microbial agent: subtilis is inoculated in bacillus subtilis bacterium culture medium and carries out activation culture, activation culture condition: temperature is 25 DEG C, and 100rpm vibrates cultivation, the time is 20h, inoculum size is 2%, obtains the subtilis seed liquor activated;
The subtilis seed liquor of above-mentioned activation is inoculated in the fermentor tank being provided with subtilis enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: inoculum size is with 3%, pH controls 7.0, carry out aerobic fermentation, fermentation ventilation rate fermentation ventilation rate 1 ︰ 0.5, fermentor tank tank pressure 0.02 MPa, temperature 30 DEG C, cultivate 36h, dry acquisition bacillus subtilis microbial agent after enlarged culturing.
Wherein, bacillus subtilis bacterium culture medium configures according to following proportioning raw materials: shared by each composition of bacillus subtilis bacterium culture medium, subtilis substratum mass percent is glucose 2%, peptone 1.5%, sodium-chlor 0.5%, extractum carnis 0.05%, agar 2%, remaining is distilled water.
Subtilis enlarged culturing base configures according to following proportioning raw materials: the mass percent that each composition of subtilis enlarged culturing base accounts for as subtilis enlarged culturing base is: molasses 2%, corn steep liquor 0.5%, peptone 1.5%, sodium-chlor 0.5%, is left for distilled water.
The preparation method of photosynthetic bacterium microbial inoculum: photosynthetic bacterium is inoculated in photosynthetic bacteria culture medium and carries out activation culture, activation culture condition: temperature is 25 DEG C, and 100rpm vibrates cultivation, the time is 20h, and inoculum size is 3%, obtains the photosynthetic bacterium seed liquor after activating;
The photosynthetic bacterium seed liquor of above-mentioned activation is inoculated in the transparent airtight container being provided with photosynthetic bacterium enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: higher to the inoculum size of photosynthetic bacterium enlarged culturing, the inoculum size of light and bacterium seed liquor is generally 20%, and namely the ratio of light and bacterium seed liquor and photosynthetic bacterium enlarged culturing base unit weight is 1:4.PH7.5, intensity of illumination: 3000Lx are best, dry acquisition photosynthetic bacterium microbial inoculum after enlarged culturing.
Photosynthetic bacteria culture medium configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacteria culture medium accounts for photosynthetic bacteria culture medium mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, agar 2%, remaining is distilled water.
Photosynthetic bacterium enlarged culturing base configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacterium enlarged culturing base accounts for photosynthetic bacterium enlarged culturing base mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, remaining is distilled water.
By obtained yeast microbial inoculum, milk-acid bacteria microbial inoculum, denitrifying bacterium microbial inoculum, nitrite bacteria microbial inoculum, bacillus subtilis microbial agent and photosynthetic bacterium microbial inoculum, according to the obtained a kind of natural water ecological purification microbial inoculum of weight ratio mixing of 1.8:2.5:3.5:5:3.5.
Get the bad five class Lake Water 50 kilograms in suburbs, Wuhan and bed mud 10mm thereof, add ecological purification microbial inoculum and process.Before process, lake water pH is 7.98, COD172.3, total nitrogen 153.1mg/L, total phosphorus 2.75.Normal temperature about 20 DEG C is placed 10 days, and every milliliter, water body is containing effective bacterium 3.1 × 10
6, yeast 10%, milk-acid bacteria 5%, denitrifying bacterium 25%, subtilis accounts for 10%, photosynthetic bacterium 30%, other bacteriums 20%.After process, water body pH7.23, COD are 75.8 total nitrogens is 73.5mg/L, and total phosphorus is 1.04, and the thick 8mm of bed mud, water quality is clearly better.
Embodiment 2:
A preparation method for natural water ecological purification microbial inoculum, its preparation process is:
The preparation method of yeast microbial inoculum: yeast is inoculated in microzyme culture medium and carries out activation culture, the condition of activation culture is: temperature 31 DEG C, static gas wave refrigerator 36h, and the inoculum size of yeast seeds is 1.5%, obtains the yeast seed liquor of activation;
Yeast seed liquor after above-mentioned activation is inoculated in the fermentor tank being placed with yeast enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: inoculum size is 8%, pH value controls 7.1, temperature is 31 DEG C, static fermentation, intermittent stirring is carried out in 1 hour in interval, fermentation 60h, fermentor tank tank pressure 0.04 MPa, dry acquisition yeast microbial inoculum after enlarged culturing;
Wherein, microzyme culture medium configures according to following proportioning raw materials: sucrose 50g, K
2hPO
45g, MgSO
42g, (NH
4)
2sO
43g, agar 15g, distilled water 1L.
Yeast enlarged culturing base configures according to following proportioning raw materials: shared by each composition of yeast enlarged culturing base, yeast enlarged culturing base mass percent is: molasses 1.5%, Rice & peanut milk 0.4%, K
2hPO
40.5%, MgSO
40.2%, (NH
4)
2sO
40.3%, be left for distilled water.
The preparation method of milk-acid bacteria microbial inoculum:
Lactobacillus inoculum is carried out activation culture in lactic acid bacteria culturing medium, and the condition of activation culture is: temperature 31 DEG C, and 26h is cultivated in 165rpm jolting, and inoculum size is 1.2%, obtains the lactobacillus solution of activation;
The above-mentioned lactobacillus solution by activation is inoculated in the fermentor tank being placed with milk-acid bacteria enlarged culturing base and carries out enlarged culturing, enlarged culturing condition: inoculum size is 8%, pH value controls 7, aerobic fermentation, fermentation ventilation rate is 1 ︰ 0.7, fermentor tank tank pressure 0.03 MPa, temperature 30 DEG C, cultivate 45h, dry acquisition milk-acid bacteria microbial inoculum after enlarged culturing;
Wherein, lactic acid bacteria culturing medium configures according to following proportioning raw materials: it is peptone 0.5% that each composition of lactic acid bacteria culturing medium accounts for lactic acid bacteria culturing medium mass percent, extractum carnis 0.5%, yeast extract paste 0.5%, glucose 2%, lactose 2%, CaCO
31%, agar 1.5%, toluylene red 0.005%, is left for distilled water;
Milk-acid bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of milk-acid bacteria enlarged culturing base, milk-acid bacteria enlarged culturing base mass percent is: molasses 1.5%, corn steep liquor 0.4%, CaCO
31%, toluylene red 0.005%, is left for distilled water.
The preparation method of denitrifying bacterium microbial inoculum: denitrifying bacterium is inoculated in denitrifying bacteria substratum and carries out activation culture, the condition of activation culture is: temperature 30 DEG C, 150rpm jolting, and cultivate 36h, inoculum size is 1.2%, obtains the denitrifying bacterium seed liquor of activation;
The denitrifying bacteria seed liquor of above-mentioned activation is inoculated in the fermentor tank being placed with denitrifying bacteria enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: by inoculum size 10%, pH controls 7.1, aerobic fermentation, fermentation ventilation rate 1 ︰ 0.65 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.03 MPa, temperature is 31 DEG C, cultivate 37h, dry acquisition denitrifying bacterium microbial inoculum after enlarged culturing.
Wherein, denitrifying bacteria substratum configures according to following proportioning raw materials: the mass percent that each composition of denitrifying bacteria substratum accounts for denitrifying bacteria substratum is saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, agar 1.5%, remaining is distilled water.
Denitrifying bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of denitrifying bacteria enlarged culturing base, the mass percent of denitrifying bacteria enlarged culturing base is molasses 1.5%, corn steep liquor 0.4%, saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, is left for distilled water.
The preparation method of bacillus subtilis microbial agent: subtilis is inoculated in bacillus subtilis bacterium culture medium and carries out activation culture, activation culture condition: temperature is 27 DEG C, and 150rpm vibrates cultivation, the time is 22h, inoculum size is 2.5%, obtains the subtilis seed liquor activated;
The subtilis seed liquor of above-mentioned activation is inoculated in the fermentor tank being provided with subtilis enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: inoculum size is with 7%, pH controls 7.2, carry out aerobic fermentation, fermentation ventilation rate fermentation ventilation rate 1 ︰ 0.6, fermentor tank tank pressure 0.03 MPa, temperature 31 DEG C, cultivate 37h, dry acquisition bacillus subtilis microbial agent after enlarged culturing.
Wherein, bacillus subtilis bacterium culture medium configures according to following proportioning raw materials: shared by each composition of bacillus subtilis bacterium culture medium, subtilis substratum mass percent is glucose 2%, peptone 1.5%, sodium-chlor 0.5%, extractum carnis 0.05%, agar 2%, remaining is distilled water.
Subtilis enlarged culturing base configures according to following proportioning raw materials: the mass percent that each composition of subtilis enlarged culturing base accounts for as subtilis enlarged culturing base is: molasses 1.5%, corn steep liquor 0.4%, peptone 1.5%, sodium-chlor 0.5%, is left for distilled water.
The preparation method of photosynthetic bacterium microbial inoculum: photosynthetic bacterium is inoculated in photosynthetic bacteria culture medium and carries out activation culture, activation culture condition: temperature is 30 DEG C, and 150rpm vibrates cultivation, the time is 22h, and inoculum size is 4%, obtains the photosynthetic bacterium seed liquor after activating;
The photosynthetic bacterium seed liquor of above-mentioned activation is inoculated in the transparent airtight container being provided with photosynthetic bacterium enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: higher to the inoculum size of photosynthetic bacterium enlarged culturing, the inoculum size of light and bacterium seed liquor is generally 35%, and namely the ratio of light and bacterium seed liquor and photosynthetic bacterium enlarged culturing base unit weight is 1:2.PH8, intensity of illumination: 3500Lx are best, cultivate 37h, dry acquisition photosynthetic bacterium microbial inoculum after enlarged culturing.
Photosynthetic bacteria culture medium configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacteria culture medium accounts for photosynthetic bacteria culture medium mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, agar 2%, remaining is distilled water.
Photosynthetic bacterium enlarged culturing base configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacterium enlarged culturing base accounts for photosynthetic bacterium enlarged culturing base mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, remaining is distilled water.
By obtained yeast microbial inoculum, milk-acid bacteria microbial inoculum, denitrifying bacterium microbial inoculum, nitrite bacteria microbial inoculum, bacillus subtilis microbial agent and photosynthetic bacterium microbial inoculum, according to the obtained a kind of natural water ecological purification microbial inoculum of weight ratio mixing of 2:2.4:3.7:6:3.4.
Get the bad five class Lake Water 70 kilograms in Wuhan urban district and bed mud 50mm thereof, add ecological purification microbial inoculum and process.Before process, lake water pH is 7.84, COD161.5, total nitrogen 193.1mg/L, total phosphorus 4.95.Normal temperature about 20 DEG C is placed 10 days, and every milliliter, water body is containing effective bacterium 2.3 × 10
6, yeast 6%, milk-acid bacteria 9%, denitrifying bacterium 15%, subtilis accounts for 20%, photosynthetic bacterium 35%, other bacteriums 15%, and after process, water body pH7.12, COD are 71.2 total nitrogens is 83.5mg/L, and total phosphorus is 2.01, and the thick 38mm of bed mud, water quality is clearly better.
Embodiment 3:
A preparation method for natural water ecological purification microbial inoculum, its preparation process is:
The preparation method of yeast microbial inoculum: yeast is inoculated in microzyme culture medium and carries out activation culture, the condition of activation culture is: temperature 32 DEG C, static gas wave refrigerator 48h, and the inoculum size of yeast seeds is 2%, obtains the yeast seed liquor of activation;
Yeast seed liquor after above-mentioned activation is inoculated in the fermentor tank being placed with yeast enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: inoculum size is 10%, pH value controls 7.2, temperature is 32 DEG C, static fermentation, intermittent stirring is carried out in 1 hour in interval, fermentation 72h, fermentor tank tank pressure 0.05 MPa, dry acquisition yeast microbial inoculum after enlarged culturing;
Wherein, microzyme culture medium configures according to following proportioning raw materials: sucrose 50g, K
2hPO
45g, MgSO
42g, (NH
4)
2sO
43g, agar 15g, distilled water 1L.
Yeast enlarged culturing base configures according to following proportioning raw materials: shared by each composition of yeast enlarged culturing base, yeast enlarged culturing base mass percent is: molasses 1.0%, Rice & peanut milk 0.3%, K
2hPO
40.5%, MgSO
40.2%, (NH
4)
2sO
40.3%, be left for distilled water.
The preparation method of milk-acid bacteria microbial inoculum:
Lactobacillus inoculum is carried out activation culture in lactic acid bacteria culturing medium, and the condition of activation culture is: temperature 32 DEG C, and 48h is cultivated in 180rpm jolting, and inoculum size is 1.5%, obtains the lactobacillus solution of activation;
The above-mentioned lactobacillus solution by activation is inoculated in the fermentor tank being placed with milk-acid bacteria enlarged culturing base and carries out enlarged culturing, enlarged culturing condition: inoculum size is 10%, pH value controls 7.2, aerobic fermentation, fermentation ventilation rate is 1 ︰ 0.8, fermentor tank tank pressure 0.05 MPa, temperature 31 DEG C, cultivate 50h, dry acquisition milk-acid bacteria microbial inoculum after enlarged culturing;
Wherein, lactic acid bacteria culturing medium configures according to following proportioning raw materials: it is peptone 0.5% that each composition of lactic acid bacteria culturing medium accounts for lactic acid bacteria culturing medium mass percent, extractum carnis 0.5%, yeast extract paste 0.5%, glucose 2%, lactose 2%, CaCO
31%, agar 1.5%, toluylene red 0.005%, is left for distilled water;
Milk-acid bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of milk-acid bacteria enlarged culturing base, milk-acid bacteria enlarged culturing base mass percent is: molasses 1.0%, corn steep liquor 0.3%, CaCO
31%, toluylene red 0.005%, is left for distilled water.
The preparation method of denitrifying bacterium microbial inoculum: denitrifying bacterium is inoculated in denitrifying bacteria substratum and carries out activation culture, the condition of activation culture is: temperature 32 DEG C, 200rpm jolting, and cultivate 48h, inoculum size is 1.5%, obtains the denitrifying bacterium seed liquor of activation;
The denitrifying bacteria seed liquor of above-mentioned activation is inoculated in the fermentor tank being placed with denitrifying bacteria enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: by inoculum size 12%, pH controls 7.3, aerobic fermentation, fermentation ventilation rate 1 ︰ 0.8, fermentor tank tank pressure 0.05 MPa, temperature is 32 DEG C, cultivate 38h, dry acquisition denitrifying bacterium microbial inoculum after enlarged culturing.
Wherein, denitrifying bacteria substratum configures according to following proportioning raw materials: the mass percent that each composition of denitrifying bacteria substratum accounts for denitrifying bacteria substratum is saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, agar 1.5%, remaining is distilled water.
Denitrifying bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of denitrifying bacteria enlarged culturing base, the mass percent of denitrifying bacteria enlarged culturing base is molasses 1.0%, corn steep liquor 0.3%, saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, is left for distilled water.
The preparation method of bacillus subtilis microbial agent: subtilis is inoculated in bacillus subtilis bacterium culture medium and carries out activation culture, activation culture condition: temperature is 30 DEG C, and 200rpm vibrates cultivation, the time is 24h, inoculum size is 3%, obtains the subtilis seed liquor activated;
The subtilis seed liquor of above-mentioned activation is inoculated in the fermentor tank being provided with subtilis enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: inoculum size is with 10%, pH controls 7.3, carry out aerobic fermentation, fermentation ventilation rate fermentation ventilation rate 1 ︰ 0.8, fermentor tank tank pressure 0.05 MPa, temperature 32 DEG C, cultivate 38h, dry acquisition bacillus subtilis microbial agent after enlarged culturing.
Wherein, bacillus subtilis bacterium culture medium configures according to following proportioning raw materials: shared by each composition of bacillus subtilis bacterium culture medium, subtilis substratum mass percent is glucose 2%, peptone 1.5%, sodium-chlor 0.5%, extractum carnis 0.05%, agar 2%, remaining is distilled water.
Subtilis enlarged culturing base configures according to following proportioning raw materials: the mass percent that each composition of subtilis enlarged culturing base accounts for as subtilis enlarged culturing base is: molasses 1.0%, corn steep liquor 0.3%, peptone 1.5%, sodium-chlor 0.5%, is left for distilled water.
The preparation method of photosynthetic bacterium microbial inoculum: photosynthetic bacterium is inoculated in photosynthetic bacteria culture medium and carries out activation culture, activation culture condition: temperature is 34 DEG C, and 200rpm vibrates cultivation, the time is 24h, and inoculum size is 5%, obtains the photosynthetic bacterium seed liquor after activating;
The photosynthetic bacterium seed liquor of above-mentioned activation is inoculated in the transparent airtight container being provided with photosynthetic bacterium enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: higher to the inoculum size of photosynthetic bacterium enlarged culturing, and the inoculum size of light and bacterium seed liquor is 50%.PH8.5, intensity of illumination: 4000Lx are best, cultivate 38h, dry acquisition photosynthetic bacterium microbial inoculum after enlarged culturing.
Photosynthetic bacteria culture medium configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacteria culture medium accounts for photosynthetic bacteria culture medium mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, agar 2%, remaining is distilled water.
Photosynthetic bacterium enlarged culturing base configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacterium enlarged culturing base accounts for photosynthetic bacterium enlarged culturing base mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, remaining is distilled water.
By obtained yeast microbial inoculum, milk-acid bacteria microbial inoculum, denitrifying bacterium microbial inoculum, nitrite bacteria microbial inoculum, bacillus subtilis microbial agent and photosynthetic bacterium microbial inoculum, according to the obtained a kind of natural water ecological purification microbial inoculum of volume ratio mixing of 2:2.4:3.7:6:3.4.
In Wuhan urban district, bad five class river course slant ranges are about 50m and carry out river course reparative experiment, and measure its bed mud 78mm, add ecological purification microbial inoculum every day at 0m place, upstream, dosage processes with every 100 square metres of 60g every day.Before process, lake water pH is 7.92, COD211.3, total nitrogen 187.6mg/L, total phosphorus 4.78.About normal temperature 15-21 DEG C processes 30 days continuously, within the 30th day, measures every milliliter, water body containing effective bacterium 2.1 × 10
6, yeast 7%, milk-acid bacteria 11%, denitrifying bacterium 13%, subtilis accounts for 21%, photosynthetic bacterium 33%, other bacteriums 15%, and after process, water body pH7.1, COD are 7.14 total nitrogens is 59.5mg/L, and total phosphorus is 1.34, and the thick 52mm of bed mud, water quality is clearly better.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.
Claims (2)
1. a preparation method for natural water ecological purification microbial inoculum, is characterized in that, its preparation process is:
The preparation method of yeast microbial inoculum: yeast is inoculated in microzyme culture medium and carries out activation culture, the condition of activation culture is: temperature 30 DEG C ~ 32 DEG C, static gas wave refrigerator 24h ~ 48h, the inoculum size of yeast seeds is 1 ~ 2%, obtains the yeast seed liquor of activation;
Yeast seed liquor after above-mentioned activation is inoculated in the fermentor tank being placed with yeast enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: inoculum size is 5-10%, pH value controls 7.0 ~ 7.2, temperature is 30 DEG C ~ 32 DEG C, static fermentation, intermittent stirring is carried out in 1 hour in interval, fermentation 48 ~ 72h, fermentor tank tank pressure 0.02 ~ 0.05 MPa, dry acquisition yeast microbial inoculum after enlarged culturing;
The preparation method of milk-acid bacteria microbial inoculum: lactobacillus inoculum is carried out activation culture in lactic acid bacteria culturing medium, the condition of activation culture is: temperature 30 DEG C ~ 32 DEG C, 24h ~ 48h is cultivated in 150 ~ 180rpm jolting, and inoculum size is 0.8 ~ 1.5%, obtains the lactobacillus solution of activation;
The above-mentioned lactobacillus solution by activation is inoculated in the fermentor tank being placed with milk-acid bacteria enlarged culturing base and carries out enlarged culturing, enlarged culturing condition: inoculum size is 5-10%, pH value controls 6.8 ~ 7.2, aerobic fermentation, fermentation ventilation rate is 1 ︰ 0.5 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.02 ~ 0.05 MPa, temperature 30 DEG C ± 1 DEG C, cultivate 40h ~ 50h, dry acquisition milk-acid bacteria microbial inoculum after enlarged culturing;
The preparation method of denitrifying bacterium microbial inoculum: denitrifying bacterium is inoculated in denitrifying bacteria substratum and carries out activation culture, the condition of activation culture is: temperature 28 DEG C ~ 32 DEG C, 100 ~ 200rpm jolting, cultivate 24h ~ 48h, inoculum size is 0.8 ~ 1.5%, obtains the denitrifying bacterium seed liquor of activation;
The denitrifying bacteria seed liquor of above-mentioned activation is inoculated in the fermentor tank being placed with denitrifying bacteria enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: by inoculum size 8 ~ 12%, pH controls 7.0 ~ 7.3, aerobic fermentation, fermentation ventilation rate 1 ︰ 0.5 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.02 ~ 0.05 MPa, temperature is 30 DEG C ~ 32 DEG C, cultivate 36 ~ 38h, dry acquisition denitrifying bacterium microbial inoculum after enlarged culturing;
The preparation method of bacillus subtilis microbial agent: subtilis is inoculated in bacillus subtilis bacterium culture medium and carries out activation culture, activation culture condition: temperature is 25 ~ 30 DEG C, 100rpm ~ 200rpm vibrates cultivation, time is 20 ~ 24h, inoculum size is 2% ~ 3%, obtains the subtilis seed liquor activated;
The subtilis seed liquor of above-mentioned activation is inoculated in the fermentor tank being provided with subtilis enlarged culturing base and carries out enlarged culturing, the condition of enlarged culturing is: inoculum size is with 3% ~ 10%, pH controls 7.0 ~ 7.3, carry out aerobic fermentation, fermentation ventilation rate fermentation ventilation rate 1 ︰ 0.5 ~ 1 ︰ 0.8, fermentor tank tank pressure 0.02 ~ 0.05 MPa, temperature 30 DEG C ~ 32 DEG C, cultivate 36 ~ 38h, dry acquisition bacillus subtilis microbial agent after enlarged culturing;
The preparation method of photosynthetic bacterium microbial inoculum: photosynthetic bacterium is inoculated in photosynthetic bacteria culture medium and carries out activation culture, activation culture condition: temperature is 25 ~ 34 DEG C, and 100rpm ~ 200rpm vibrates cultivation, the time is 20 ~ 24h, inoculum size is 3% ~ 5%, obtains the photosynthetic bacterium seed liquor after activating;
The photosynthetic bacterium seed liquor of above-mentioned activation is inoculated in the transparent airtight container being provided with photosynthetic bacterium enlarged culturing base and carries out enlarged culturing, enlarged culturing condition is: inoculum size 20% ~ 50%, pH7.5 ~ 8.5, intensity of illumination: dry acquisition photosynthetic bacterium microbial inoculum after 3000Lx-4000Lx enlarged culturing;
By obtained yeast microbial inoculum, milk-acid bacteria microbial inoculum, denitrifying bacterium microbial inoculum, nitrite bacteria microbial inoculum, bacillus subtilis microbial agent and photosynthetic bacterium microbial inoculum, according to the obtained a kind of natural water ecological purification microbial inoculum of weight part mixing of 1 ~ 2:1 ~ 3:3 ~ 4:4 ~ 6:2 ~ 5.
2. the preparation method of a kind of natural water ecological purification microbial inoculum according to claim 1, is characterized in that: described microzyme culture medium configures according to following proportioning raw materials: sucrose 50g, K
2hPO
45g, MgSO
42g, (NH
4)
2sO
43g, agar 15g, distilled water 1L;
Described yeast enlarged culturing base configures according to following proportioning raw materials: shared by each composition of yeast enlarged culturing base, yeast enlarged culturing base mass percent is: molasses 2% or 1.5% or 1.0%, Rice & peanut milk 0.5% or 0.4% or 0.3%, K
2hPO
40.5%, MgSO
40.2%, (NH
4)
2sO
40.3%, be left for distilled water;
Described lactic acid bacteria culturing medium configures according to following proportioning raw materials: it is peptone 0.5% that each composition of lactic acid bacteria culturing medium accounts for lactic acid bacteria culturing medium mass percent, extractum carnis 0.5%, yeast extract paste 0.5%, glucose 2%, lactose 2%, CaCO
31%, agar 1.5%, toluylene red 0.005%, is left for distilled water;
Described milk-acid bacteria enlarged culturing base configures according to following proportioning raw materials: shared by each composition of milk-acid bacteria enlarged culturing base, milk-acid bacteria enlarged culturing base mass percent is: molasses 2% or 1.5% or 1.0%, corn steep liquor 0.5% or 0.4% or 0.3%, CaCO
31%, toluylene red 0.005%, is left for distilled water;
Described denitrifying bacteria substratum configures according to following proportioning raw materials: the mass percent that each composition of denitrifying bacteria substratum accounts for denitrifying bacteria substratum is saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, agar 1.5%, remaining is distilled water;
Described denitrifying bacteria enlarged culturing base configures according to following proportioning raw materials: the mass percent of denitrifying bacteria enlarged culturing base shared by each composition of denitrifying bacteria enlarged culturing base is molasses 2% or 1.5% or 1.0%, corn steep liquor 0.5% or 0.4% or 0.3%, saltpetre 0.2%, magnesium sulfate 0.02%, potassium hydrogen phosphate 0.05%, Seignette salt 2%, be left for distilled water
Described bacillus subtilis bacterium culture medium configures according to following proportioning raw materials: shared by each composition of bacillus subtilis bacterium culture medium, subtilis substratum mass percent is glucose 2%, peptone 1.5%, sodium-chlor 0.5%, extractum carnis 0.05%, agar 2%, remaining is distilled water;
Described subtilis enlarged culturing base configures according to following proportioning raw materials: the mass percent that each composition of subtilis enlarged culturing base accounts for as subtilis enlarged culturing base is: molasses 2% or 1.5% or 1.0%, corn steep liquor 0.5% or 0.4% or 0.3%, peptone 1.5%, sodium-chlor 0.5%, be left for distilled water
Described photosynthetic bacteria culture medium configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacteria culture medium accounts for photosynthetic bacteria culture medium mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, agar 2%, remaining is distilled water;
Described photosynthetic bacterium enlarged culturing base configures according to following proportioning raw materials: it is sodium-chlor 0.1% that each composition of photosynthetic bacterium enlarged culturing base accounts for photosynthetic bacterium enlarged culturing base mass percent, dipotassium hydrogen phosphate 0.025%, magnesium sulfate 0.025%, ammonium chloride 0.1%, sodium bicarbonate 0.1%, sodium acetate 0.25%, remaining is distilled water.
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| CN107586739A (en) * | 2017-09-30 | 2018-01-16 | 南京万德斯环保科技股份有限公司 | A kind of complex microbial inoculum for purifying river course water environment and its application |
| CN108220186A (en) * | 2017-12-20 | 2018-06-29 | 无锡市拜沃特环保科技有限公司 | A kind of denitrogenation is except algae microbial bacterial agent and preparation method thereof |
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| CN111040970A (en) * | 2019-12-27 | 2020-04-21 | 河北九华勘查测绘有限责任公司 | Compound microbial agent, preparation method thereof and application thereof in black and odorous water body remediation |
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| CN114645041A (en) * | 2022-04-21 | 2022-06-21 | 江西调水人生态环境工程有限公司 | Bottom sediment-improved composite microbial inoculum and preparation method thereof |
| CN114920364A (en) * | 2022-05-24 | 2022-08-19 | 山东章威生物科技有限公司 | Mixed-strain fermentation microbial deodorant and preparation method thereof |
| CN115611434A (en) * | 2022-09-13 | 2023-01-17 | 东莞市环洁化工有限公司 | Composite carbon source for enhancing biological denitrification of sewage treatment and production method thereof |
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Application publication date: 20151209 |