CN104371959B - Biological deodorizing fly eradication composite bacteria agent and its application - Google Patents
Biological deodorizing fly eradication composite bacteria agent and its application Download PDFInfo
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Abstract
The present invention provides biological deodorizing fly eradication composite bacteria agent and its application.Biological deodorizing fly eradication composite bacteria agent includes saccharomycete, lactic acid bacteria, function bacillus, photosynthetic bacteria and muscardine, and the mass ratio of each strain is the ﹕ 1 of 0.5~2 ﹕, 0.5~2.5 ﹕, 1.5~3.5 ﹕ 1.5~2.5 in order；Microorganism live bacteria sum >=109CFU/g.The function bacillus is the mix bacterium agent for including bacillus megaterium, bacillus amyloliquefaciens, bacillus subtilis, Bacillus cercus, bacillus licheniformis, bacillus pumilus and bacillus thuringiensis.And biological deodorizing fly eradication composite bacteria agent application of deodorization and fly eradication in garbage collection procedure.The biological deodorizing fly eradication composite bacteria agent of the present invention is formed by saccharomycete, lactic acid bacteria, function bacillus, photosynthetic bacteria and muscardine reasonable compatibility, safe and efficient.The deodorization and fly eradication in the removal process of house refuse are applied, deodorization and effect of killing flies are good.
Description
Technical field
The present invention relates to biological deodorizing and fly eradication bacteria agent technical field, more particularly to suitable for domestic waste
The biological deodorizing fly eradication composite bacteria agent of processing and its application.
Background technology
With the continuous growth and growth in the living standard of urban population, municipal refuse is more and more, at the recovery of rubbish
Reason turns into the serious problems of puzzlement society.Because rubbish biological absorption method is compared with existing Landfill, waste incineration method
With occupation of land less, the problems such as not producing pollution to environment, the major way of the recycling of rubbish is turned into.
Rubbish biological recovery is main to include ragging process, broken bag process, Biological Pretreatment process, once sorting process,
Fermentation procedure, secondary sorting process, baking operation, selected process, organic fertilizer process again.Ferment in Biological Pretreatment process and again
During, stench is often accompanied by, and mosquitos and flies grows.Therefore, many biological deodorants and fly spray are have developed, for biology
In preprocessing process and again fermentation process.At present, deodorant and fly spray are two kinds of separated different products, and both
Product is often incompatible, and fly eradication product can influence the performance of deodorising product, reduces deodorizing effect.Meanwhile respectively using removing
Smelly dose and two kinds of products of fly spray, complex procedures, workload can also increase.Therefore, need badly and develop one kind and have deodorization concurrently and go out
The bacteria agent mixture of two kinds of effects of fly, it is incompatible to solve existing two kinds of products of deodorant and fly spray, and fly eradication product can shadow
The performance of deodorising product is rung, reduces deodorizing effect, and use two kinds of products of deodorant and fly spray, complex procedures, work respectively
Measuring also can increased technical problem.
The content of the invention
For the drawbacks described above and problem of prior art, it is an object of the invention to provide biological deodorizing fly eradication composite bacteria agent and
It is applied.Solve that existing two kinds of products of deodorant and fly spray are incompatible, and fly eradication product can influence the performance of deodorising product, reduce
Deodorizing effect, and use two kinds of products of deodorant and fly spray respectively, complex procedures, workload also can increased technology ask
Topic.
In order to achieve the above object, the present invention provides following technical scheme：
Biological deodorizing fly eradication composite bacteria agent, including S. cervisiae, lactic acid bacteria, function bacillus, photosynthetic bacteria and white
Stiff bacterium, the mass ratio of each strain is the ﹕ 1.0 of 0.5~2.0 ﹕, 0.5~2.5 ﹕, 1.5~3.5 ﹕ 1.5~2.5 in order；Microorganism live bacteria
Sum >=109CFU/g；The function bacillus is to include bacillus megaterium, bacillus amyloliquefaciens, bacillus subtilis
Bacterium, Bacillus cercus, bacillus licheniformis, the mix bacterium agent of bacillus pumilus and bacillus thuringiensis.
Further, the mass ratio of each strain is the ﹕ 1.0 of 1.0~1.5 ﹕, 1.0~2.0 ﹕, 2.0~3.0 ﹕ 2.0 in order.
Further, the function bacillus is to include bacillus megaterium, bacillus amyloliquefaciens, bacillus subtilis
Bacterium, Bacillus cercus, bacillus licheniformis, the mix bacterium agent of bacillus pumilus and bacillus thuringiensis；It is each in order
The mass ratio of strain is 0.5~2.5: 0.5~2.0: 0.5~2.0: 0.1~1.0: 0.1~1.5: 0.1~1.5: 0.5~3.0,
Bacteria containing amount >=1.0 × 1010CFU/ grams.
Further, in the function bacillus, the mass ratio of each strain is 0.5~2.5: 0.5~1.0 in order:
0.5~2.0: 0.5~0.8: 1.0~1.5: 0.5~1.5: 1.5~3.0.It is highly preferred that the mass ratio of each strain is in order
2.5∶0.5∶1.0∶0.5∶1.0∶1.5∶3.0。
Further, the lactic acid bacteria uses Lactobacillus plantarum.The photosynthetic bacteria uses Rhodopseudomonas palustris.It is described
Muscardine uses beauveria bassiana.
Biological deodorizing fly eradication composite bacteria agent application of deodorization and fly eradication in garbage collection procedure of the present invention.
The biological deodorizing fly eradication composite bacteria agent of the present invention removes in domestic waste Biological Pretreatment and again fermentation process
The smelly and application of fly eradication.
Further, the biological deodorizing fly eradication composite bacteria agent of the present invention is diluted into obtain composite bacteria liquid, composite bacteria liquid is added
In pending rubbish, then according to Biological Pretreatment or again, zymotechnique is handled；Wherein, the composite bacteria liquid volume of addition
Ratio with pending rubbish quality is 0.5~1: 100.
Further, it is 1 by the mass ratio of biological deodorizing fly eradication composite bacteria agent and water:50~100 ratio, biology is removed
Smelly fly eradication composite bacteria agent dilutes to obtain composite bacteria liquid.
The equal reference of expansion culture medium of strain used in the biological deodorizing fly eradication composite bacteria agent of the present invention《CGMCC strain mesh
Record》.After each strain is expanded into culture respectively, mixed according to proportion compatibility.
The present invention biological deodorizing fly eradication composite bacteria agent by saccharomycete, lactic acid bacteria, function bacillus, photosynthetic bacteria and
Muscardine reasonable compatibility forms, safe and efficient.Applied the deodorization and fly eradication in the removal process of house refuse, deodorization and gone out
Fly effect is good.
In the biological deodorizing fly eradication composite bacteria agent of the present invention S. cervisiae and function bacillus can extracellular proteinase,
A variety of enzymes such as lipase and cellulase, so as to municipal domestic organic refuse of degrading.Lactobacillus-fermented production acid, can suppress cause of disease
The growth of microorganism.Simultaneously parasporal crystal (e.g., bacillus thuringiensis), its crystalline protein can be produced in function bacillus
There is toxic action to insects such as mosquito, flies.Meanwhile muscardine can invade amount reproduction in the polypide of various insects, mite class, together
When constantly produce white stiff plain (big ring grease toxoid) and calcium oxalate crystal, these materials and insect can be caused to be poisoned, find body fluid
Function changes, and upsets metabolism so that dead.Photosynthetic bacteria cell directly produces degraded ammonia and sulphur in growth course
Change the enzyme of hydrogen, it is thus possible to the gas with foreign flavor such as caused ammonia and hydrogen sulfide in fast decoupled rubbish.
The function bacillus is to include bacillus megaterium, bacillus amyloliquefaciens, bacillus subtilis, wax-like bud
Spore bacillus, bacillus licheniformis, the mix bacterium agent of bacillus pumilus and bacillus thuringiensis.In domestic waste,
Starch, albumen, carbohydrate (cellulose) and fat are main compositions, mix bacterium agent of the function bacillus as a variety of strains,
By its reasonable compatibility, it can produce amylase, protease, lipase and cellulase, it is thus possible to rubbish is thoroughly decomposed,
The formation that supernatant eliminates the unusual smell from source.Bacillus thuringiensis therein can produce parasporal crystal simultaneously, and its crystalline protein is only
There is toxic action to insects such as mosquito, flies, do not have inhibitory action to bacterium.Function bacillus coordinates other saccharomyces cerevisiaes
Bacterium, lactic acid bacteria, muscardine and photosynthetic bacteria, deodorization fly eradication function is realized jointly.
The biological deodorizing fly eradication composite bacteria agent of the present invention applies the deodorization and fly eradication in the removal process of house refuse, deodorization
It is good with effect of killing flies.The clearance of ammonia reaches more than 80%, and methylamine goes out clearance and is up to 96%, and indoles clearance is up to
82%, hydrogen sulfide removal rate is up to 95%.Mosquitos and flies clearance reaches more than 75%.
Embodiment
Below in conjunction with embodiments of the invention, technical scheme is clearly and completely described, it is clear that
Described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based on the implementation in the present invention
Example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, is belonged to
The scope of protection of the invention.
Embodiment 1
The biological deodorizing fly eradication composite bacteria agent of the embodiment of the present invention 1, including S. cervisiae, Lactobacillus plantarum, function bud
Spore bacillus, Rhodopseudomonas palustris and beauveria bassiana, the mass ratio of each strain is the ﹕ 1 of 1.5 ﹕, 1 ﹕, 2 ﹕ 2 in order；Microorganism lives
Bacterium sum >=1.0 × 109CFU/g。
Wherein, the function bacillus is to include bacillus megaterium (Bacillus megetarium), solution starch bud
Spore bacillus (Bacillus amyloliquefaciens), bacillus subtilis (Bacillus subtilis), wax-like gemma bar
Bacterium (Bacillus cereus), bacillus licheniformis (Bacillus licheniformis), bacillus pumilus
(Bacillus pumilus) and the mix bacterium agent of bacillus thuringiensis (Bacillus thuringiensis)；It is each in order
The mass ratio of strain is 0.5: 1.0: 2.0: 0.5: 1.5: 1.0: 2.0, bacteria containing amount >=1.0 × 1010CFU/ grams.
Embodiment 2
The biological deodorizing fly eradication composite bacteria agent of the embodiment of the present invention 2, including S. cervisiae, Lactobacillus plantarum, function bud
Spore bacillus, Rhodopseudomonas palustris and beauveria bassiana, the mass ratio of each strain is the ﹕ 1 of 1 ﹕, 2 ﹕, 2.5 ﹕ 2 in order；Microorganism lives
Bacterium sum >=1.0 × 109CFU/g。
Wherein, the function bacillus is to include bacillus megaterium (Bacillus megetarium), solution starch bud
Spore bacillus (Bacillus amyloliquefaciens), bacillus subtilis (Bacillus subtilis), wax-like gemma bar
Bacterium (Bacillus cereus), bacillus licheniformis (Bacillus licheniformis), bacillus pumilus
(Bacillus pumilus) and the mix bacterium agent of bacillus thuringiensis (Bacillus thuringiensis)；It is each in order
The mass ratio of strain is 1.5: 1.0: 0.5: 0.8: 1.2: 1.0: 1.5, bacteria containing amount >=1.0 × 1010CFU/ grams.
Embodiment 3
The biological deodorizing fly eradication composite bacteria agent of the embodiment of the present invention 3, including S. cervisiae, Lactobacillus plantarum, function bud
Spore bacillus, Rhodopseudomonas palustris and beauveria bassiana, the mass ratio of each strain is the ﹕ 1 of 1.5 ﹕, 1 ﹕, 2.5 ﹕ 2 in order；Microorganism
Total viable count >=1.0 × 109CFU/g。
Wherein, the function bacillus is to include bacillus megaterium (Bacillus megetarium), solution starch bud
Spore bacillus (Bacillus amyloliquefaciens), bacillus subtilis (Bacillus subtilis), wax-like gemma bar
Bacterium (Bacillus cereus), bacillus licheniformis (Bacillus licheniformis), bacillus pumilus
(Bacillus pumilus) and the mix bacterium agent of bacillus thuringiensis (Bacillus thuringiensis)；It is each in order
The mass ratio of strain is 0.5: 1.0: 1.0: 0.5: 1.5: 0.5: 2.0, bacteria containing amount >=1.0 × 1010CFU/ grams.
Embodiment 4
The biological deodorizing fly eradication composite bacteria agent of the embodiment of the present invention 4, including S. cervisiae, Lactobacillus plantarum, function bud
Spore bacillus, Rhodopseudomonas palustris and beauveria bassiana, the mass ratio of each strain is the ﹕ 1 of 1 ﹕, 1.5 ﹕, 3 ﹕ 2 in order；Microorganism lives
Bacterium sum >=1.0 × 109CFU/g。
Wherein, the function bacillus is to include bacillus megaterium (Bacillus megetarium), solution starch bud
Spore bacillus (Bacillus amyloliquefaciens), bacillus subtilis (Bacillus subtilis), wax-like gemma bar
Bacterium (Bacillus cereus), bacillus licheniformis (Bacillus licheniformis), bacillus pumilus
(Bacillus pumilus) and the mix bacterium agent of bacillus thuringiensis (Bacillus thuringiensis)；It is each in order
The mass ratio of strain is 2.5: 0.5: 1.0: 0.5: 1.0: 1.5: 3.0, bacteria containing amount >=1.0 × 1010CFU/ grams.
The source for each strain that above-described embodiment 1 uses to the biological deodorizing fly eradication composite bacteria agent of the invention of embodiment 4
It is as follows：
S. cervisiae (Saccharomyces cerevisiae) is bought in China General Microbiological culture presevation management
Center, preserving number CGMCC2.4；
Lactobacillus plantarum (Lactobacillus plantarum) is bought in China General Microbiological culture presevation management
The heart, preserving number CGMCC1.555；
Rhodopseudomonas palustris (Rhodosedoseudomouas padustris) is bought in Chinese agriculture microorganism fungus kind
Preservation administrative center (ACCC), preserving number ACCC10649；
Beauveria bassiana (Beauveria bassiana) is bought in China General Microbiological culture presevation administrative center, is protected
Tibetan number is CGMCC 3.4273；
Bacillus megaterium (Bacillus megetarium) is bought in China General Microbiological culture presevation management
The heart, preserving number CGMCC1.2393；
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are bought to be protected in Chinese industrial microorganism fungus kind
Hide administrative center, preserving number CICC20606；
Bacillus subtilis (Bacillus subtilis) is bought in China General Microbiological culture presevation administrative center,
Preserving number is CGMCC1.504；
Bacillus cercus (Bacillus cereus) is bought in China General Microbiological culture presevation administrative center, is protected
Tibetan number is CGMCC1.2224；
Bacillus licheniformis (Bacillus licheniformis) is bought in China General Microbiological culture presevation management
Center, preserving number CGMCC1.813；
Bacillus pumilus (Bacillus pumilus) is bought in China General Microbiological culture presevation administrative center, protects
Tibetan number is CGMCC1.937；
Bacillus thuringiensis uses bacillus thuringiensis subsp israelensis (Bacillus thuringiensis
Subsp.israelensis) buy in China General Microbiological culture presevation administrative center, preserving number CGMCC1.1754.
Application example in the Biological Pretreatment process of embodiment 5
The biological deodorizing fly eradication composite bacteria agent of the invention of embodiment 1 to embodiment 4 is given birth to applied to domestic waste
Deodorization and fly eradication in thing preprocessing process.
The biological deodorizing fly eradication composite bacteria agent of the invention of embodiment 1 to embodiment 4 is added to the water, dilutes to obtain compound bacteria
The mass ratio of liquid, biological deodorizing fly eradication composite bacteria agent and water is 1:100；Then composite bacteria liquid is added in pending rubbish, so
Handled afterwards according to existing biological pre-treatment process；Wherein, the composite bacteria liquid volume of addition and pending rubbish quality
Ratio is 1: 100.Gas after processing is detected.Mosquitos and flies quantity caused by detection simultaneously.
Experiment 1 is compared, the biological deodorizing fly eradication composite bacteria agent of the present invention is not added in pending rubbish, according to existing
Biological pre-treatment process handled.Gas after contrast test processing is detected.Mosquitos and flies caused by detection simultaneously
Quantity.
On the basis of the content of each composition in gas after the processing of contrast test 1, embodiment 1 is calculated to embodiment 4
The deodorizing effect of biological deodorizing fly eradication composite bacteria agent, as a result as shown in table 1, effect of killing flies is as shown in table 1.
Table 1
Wherein, the criterion of effect of killing flies is：The biological deodorizing fly eradication that embodiment 1 to embodiment 4 is used continuously is compound
After microbial inoculum 5 days, it is outstanding that the decrement of mosquitos and flies, which reaches more than 85%, and it is good to reach more than 75%.
The application example in fermentation procedure again of embodiment 6
The biological deodorizing fly eradication composite bacteria agent of the invention of embodiment 1 to embodiment 4 is given birth to applied to domestic waste
Deodorization and fly eradication in thing preprocessing process.
The biological deodorizing fly eradication composite bacteria agent of the invention of embodiment 1 to embodiment 4 is added to the water, dilutes to obtain compound bacteria
The mass ratio of liquid, biological deodorizing fly eradication composite bacteria agent and water is 1:50；Then composite bacteria liquid is added in pending rubbish, then
Handled according to existing zymotechnique again；Wherein, the ratio of the composite bacteria liquid volume of addition and pending rubbish quality is
0.5∶100.Gas after processing is detected.Mosquitos and flies quantity caused by detection simultaneously.
Experiment 2 is compared, the biological deodorizing fly eradication composite bacteria agent of the present invention is not added in pending rubbish, according to existing
Zymotechnique again handled.Gas after contrast test processing is detected.Mosquitos and flies quantity caused by detection simultaneously.
On the basis of the content of each composition in gas after the processing of contrast test 2, embodiment 1 is calculated to embodiment 4
The deodorizing effect of biological deodorizing fly eradication composite bacteria agent, as a result as shown in table 2, effect of killing flies is as shown in table 2.
Table 2
Wherein, the criterion of effect of killing flies is the same as embodiment 5.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.
Claims (5)
1. biological deodorizing fly eradication composite bacteria agent, it is characterised in that：By S. cervisiae, lactic acid bacteria, function bacillus, photosynthetic thin
Bacterium and muscardine composition, the mass ratio of each strain is the ﹕ 1.0 of 1.0 ﹕, 1.5 ﹕, 3.0 ﹕ 2.0 in order；Microorganism live bacteria sum >=
109CFU/g；
The S. cervisiae is the S. cervisiae (Saccharomyces cerevisiae) that preserving number is CGMCC2.4；
The lactic acid bacteria uses Lactobacillus plantarum, is the Lactobacillus plantarum (Lactobacillus that preserving number is CGMCC1.555
plantarum)；
The function bacillus is by bacillus megaterium, bacillus amyloliquefaciens, bacillus subtilis, wax-like gemma bar
The quality of mix bacterium agent, in order each strain that bacterium, bacillus licheniformis, bacillus pumilus and bacillus thuringiensis form
Than for 2.5: 0.5: 1.0: 0.5: 1.0: 1.5: 3.0, bacteria containing amount >=1.0 × 1010CFU/ grams；
The bacillus megaterium be preserving number be CGMCC1.2393 bacillus megaterium (Bacillus megetarium)；
The bacillus amyloliquefaciens be preserving number be CICC20606 bacillus amyloliquefaciens (Bacillus amyloliquefaciens)；
The bacillus subtilis be preserving number be CGMCC1.504 bacillus subtilis (Bacillus subtilis)；
Said waxy bacillus be preserving number be CGMCC1.2224 Bacillus cercus (Bacillus cereus)；
The bacillus licheniformis be preserving number be CGMCC1.813 bacillus licheniformis (Bacillus licheniformis)；
The bacillus pumilus be preserving number be CGMCC1.937 bacillus pumilus (Bacillus pumilus)；
The bacillus thuringiensis uses bacillus thuringiensis subsp israelensis, is the Soviet Union that preserving number is CGMCC1.1754
Cloud gold bacillus Israel subclass (Bacillus thuringiensis subsp.israelensis)；
The photosynthetic bacteria uses Rhodopseudomonas palustris, is the Rhodopseudomonas palustris that preserving number is ACCC10649
(Rhodosedoseudomouas padustris)；
The muscardine uses beauveria bassiana, be preserving number be CGMCC 3.4273 beauveria bassiana (Beauveria bassiana)。
2. biological deodorizing fly eradication composite bacteria agent as claimed in claim 1 application of deodorization and fly eradication in garbage collection procedure.
3. biological deodorizing fly eradication composite bacteria agent according to claim 2 in garbage collection procedure deodorization and fly eradication should
With, it is characterised in that：The deodorization in domestic waste Biological Pretreatment and again fermentation process of biological deodorizing fly eradication composite bacteria agent
With the application of fly eradication.
4. biological deodorizing fly eradication composite bacteria agent according to claim 3 in garbage collection procedure deodorization and fly eradication should
With, it is characterised in that：Biological deodorizing fly eradication composite bacteria agent is diluted into obtain composite bacteria liquid, composite bacteria liquid is added into pending rubbish
In, then according to Biological Pretreatment or again, zymotechnique is handled；Wherein, the composite bacteria liquid volume of addition and pending rubbish
The ratio of rubbish quality is 0.5~1: 100.
5. biological deodorizing fly eradication composite bacteria agent according to claim 4 in garbage collection procedure deodorization and fly eradication should
With, it is characterised in that：It is 1 by the mass ratio of biological deodorizing fly eradication composite bacteria agent and water:50~100 ratio, by biological deodorizing
Fly eradication composite bacteria agent dilutes to obtain composite bacteria liquid.
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| CN104371959B true CN104371959B (en) | 2017-12-05 |
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