CN1370829A - Bacterial spawn for treating domestic organic garbage - Google Patents
Bacterial spawn for treating domestic organic garbage Download PDFInfo
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- CN1370829A CN1370829A CN 01107596 CN01107596A CN1370829A CN 1370829 A CN1370829 A CN 1370829A CN 01107596 CN01107596 CN 01107596 CN 01107596 A CN01107596 A CN 01107596A CN 1370829 A CN1370829 A CN 1370829A
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Abstract
The present invention relates to a mixture of several kinds of bacterial spawns, especially bacterial spawn mixture capable of treating domestic organic garbage. The mixture consists of Bacillus megaterium Bacillus cereus, Bacillus laterosporus, Bacillus lichen formis, Bacillus thuringiensis, Bacillus pumilus, Bacillus thermophilus and Bacillus subtilis generating proteinanse, lipase, amylase, chitinase, cellulase, oxidase, etc. separately to decompose macromolecular matter into low molecular matters and to convert the low molecular matters into CO2, H2O and small amount of NH3 of exhaust while releasing energy and eliminating bad smell and pollution. The product invention is used to reduce amount of garbage.
Description
The present invention relates to the mixture of several bacterial classifications, especially several bacterial classification mixtures of handling domestic organic garbage.
Along with the continuous growth and the growth in the living standard of urban population, domestic waste every year increases rapidly with 8-10%'s, and wherein over half is organic waste.The method that this class rubbish is handled in present countries in the world mainly contains landfill method, burning method and composting process, but this several method all has its weak point, or takies a large amount of soils, or causes surface water and phreatic pollution, or causes the pollution of air and surrounding environment.
The object of the present invention is to provide the several bacterial classifications that can handle domestic organic garbage, these effective bacterial classifications can produce proteolytic enzyme, lipase, amylase, chitin enzyme (chitinase), cellulase, oxydase etc. respectively, thereby make macromolecular substance (main component of domestic organic garbage) be become lower-molecular substance by microbiological degradation, behind these lower-molecular substances of microorganism panning, be converted into CO
2, H
2O and minor N H
3(and releasing energy) discharges with the form of gas and steam, effectively eliminates odor pollution, and makes domestic organic garbage (solid-state form) reach decrement.
The present invention is mixed by certain proportioning by following bacterial classification to finish its goal of the invention: bacillus megaterium (Bacillus megetarium), bacillus cereus (Bacillus cereus), bacillus laterosporus (Bacillus laterosporus), Bacillus licheniformis (Bacilluslicheniformis), bacillus thuringiensis (Bacillus thuringiensis), bacillus pumilus (bacillus pumilus), bacstearothermophilus (bacillusstearothermophilus), subtilis (bacillus subtilis), its proportioning in order is 0.1-2.5: 0.5-1.5: 0.1-1.5: 0.1-2.0: 0.1-3: 0.1-2: 0.1-2.5: 0.1-2.5 (proportioning of each bacterial classification is to be unit with weight), eight kinds of biologically active substances that effective microbe is composited after pure culture, bacteria containing amount 〉=2.0 * 10
11Individual/gram, face when using and mix.
Bacterial classification of the present invention is a wild-type, and its type species have at the bacterial classification center of preserving, and above bacterial classification is that US mode DSMZ all has preservation in Institute of Microorganism, Academia Sinica and ATCC (American Type Culture Collection).The bacterial classification that U.S.'s strain library is preserved is if patent bacterial classification (patentstrain) has been established a capital numbering, be a bacterial strain of clostridium acetobutylicum or claim strain (strain) that as Clostridium acetobutylicum ATCC 824 wild-type bacterial classification or type culture then differ to establish a capital needs numbering.Wild-type bacterial classification involved in the present invention can reach the effect of effective processing domestic organic garbage.Patent of the present invention is based on the synergy of mixed strains and flora is set up the stable ecosystem when the disaggregating treatment domestic organic garbage the basis.And this stable ecosystem can not influence its treatment effect because of external microorganism (carrying in the domestic organic garbage).
The mycology feature of the bacterial classification of each bacterial classification is as follows:
Below be bacillus megaterium (Bacillus megetarium) 1) formal name used at school; Bacillus megaterium (Bacillus megetarium) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) bacillus megaterium (Bacillus megetarium) 3) other title (popular name, strain name, kind name);
TW-1 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 1.2-1.5, long (micron) 2-5.Gram-reaction+.In the protoplasma not colored particles+.Gemma: ellipse, middle life.Catalase+.Catalase+.Anaerobic growth+.Methyl red test+.The V.P reaction-.Growth temperature (℃) 5-45.7% NaCl growth.The growth of PH5.7 substratum.Glucose, wood sugar, pectinose, N.F,USP MANNITOL produce acid.Utilize not aerogenesis of glucose.The starch hydrolysis.Utilize Citrate trianion.Nitrate reduction.Decompose casein.Hydrolysis tyrosine.Gelatine liquefication.TW-1 bacillus megaterium (Bacillus megetarium) is decomposed greengrocery, meat dish class in 24 hours substantially, and is to decompose the meat dish class, more obvious with other bacterial classification result of use.
Below be bacillus cereus (Bacillus cereus) 1) formal name used at school;
Bacillus cereus (Bacillus cereus) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) bacillus cereus (Bacillus cereus) 3) other title (popular name, strain name, kind name);
TW-2 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 1.0-1.2, long (micron) 3-5.Gram-reaction+.In the protoplasma not colored particles+.Gemma: ellipse, inferior end is given birth to.Catalase+.Catalase+.Anaerobic growth+.Methyl red test+.The V.P reaction+.Growth temperature (℃) 10-50.7% NaCl growth.The growth of PH5.7-9 substratum.Glucose produces acid.Pectinose, wood sugar, N.F,USP MANNITOL do not produce acid.Utilize not aerogenesis of glucose.The starch hydrolysis.Do not utilize Citrate trianion.Nitrate reduction.Decompose casein.Hydrolysis tyrosine.Gelatine liquefication.TW-2 bacillus cereus (Bacillus cereus) decomposes greengrocery, meat dish class in 20 hours substantially, and is to decompose the meat dish class, more obvious with other bacterial classification result of use.
Following content is an example 1 with bacillus laterosporus (Bacillus laterosporus)) formal name used at school;
Bacillus laterosporus (Bacillus laterosporus) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) bacillus laterosporus (Bacillus laterosporus) 3) other title (popular name, strain name, kind name);
TW-3 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 0.5-0.8, long (micron) 2-5.Gram-reaction+.Gemma: ellipse, expand, middle Sheng-Ya holds and gives birth to.Catalase+.Catalase+.Anaerobic growth+.Methyl red test+.The V.P reaction-.Growth temperature (℃) 15-45.5% NaCl growth.The growth of PH5.7 substratum.Glucose produces acid.Pectinose, wood sugar, N.F,USP MANNITOL do not produce acid.Utilize not aerogenesis of glucose.Hydrolyzed starch not.Do not utilize Citrate trianion.Nitrate reduction.Decompose casein.Hydrolysis tyrosine.Gelatine liquefication.TW-3 bacillus laterosporus (Bacillus laterosporus) decomposes greengrocery, meat dish class in 24 hours substantially, and is to decompose greengrocery, more obvious with other bacterial classification result of use.
Below be Bacillus licheniformis (Bacillus licheniformis) 1) formal name used at school;
Bacillus licheniformis (Bacillus licheniformis) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) Bacillus licheniformis (Bacillus licheniformis) 3) other title (popular name, strain name, kind name);
TW-4 (TW is the phonetic alphabet abbreviation of the luxuriant Bioisystech Co., Ltd in sky, and numeral is numbering) 4) description of evaluation and Monitoring techniques;
Thalline: wide (micron) 0.6-1.0, long (micron) 1.5-3.Gram-reaction+.In the protoplasma not colored particles-.Gemma: ellipse, inferior end is given birth to or end is given birth to.Catalase+.Catalase+.Anaerobic growth+.Methyl red test-.The V.P reaction+.Growth temperature (℃) 15-55.7% NaCl growth.The growth of PH5.7-9 substratum.Glucose, pectinose, wood sugar, N.F,USP MANNITOL produce acid.Utilize not aerogenesis of glucose.The starch hydrolysis.Utilize Citrate trianion.Propionic salt utilizes.Nitrate reduction.Decompose casein.Gelatine liquefication.TW-4 Bacillus licheniformis (Bacillus licheniformis) is decomposed greengrocery, meat dish class in 20 hours substantially, and is to decompose meat dish class and carbohydrate, more obvious with other bacterial classification result of use.
Below be bacillus thuringiensis (Bacillus thuringiensis) 1) formal name used at school;
Bacillus thuringiensis (Bacillus thuringiensis) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) bacillus thuringiensis (Bacillus thuringiensis) 3) other title (popular name, strain name, kind name);
TW-5 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 1.0-1.2, long (micron) 3-5.Gram-reaction+.In the protoplasma not colored particles+.Gemma: ellipse, middle Sheng-Ya are held and are given birth to.Catalase+.Catalase+.Anaerobic growth+.Methyl red test+.The V.P reaction-.Growth temperature 10-45 (℃).5-7% NaCl growth.The growth of PH5.7-9 substratum.Glucose produces acid.Pectinose, wood sugar, N.F,USP MANNITOL do not produce acid.Utilize not aerogenesis of glucose.Hydrolyzed starch not.Citrate trianion utilization or do not utilize.Nitrate reduction.Decompose casein.Hydrolysis tyrosine.Gelatine liquefication.TW-5 bacillus thuringiensis (Bacillus thuringiensis) is decomposed greengrocery, meat dish class in 24 hours substantially, and is based on decomposing protein, more obvious with other bacterial classification result of use.
Below be bacillus pumilus (bacillus pumilus) 1) formal name used at school;
Bacillus pumilus (bacillus pumilus) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) bacillus pumilus (bacillus pumilus) 3) other title (popular name, strain name, kind name);
TW-6 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 0.6-0.7, long (micron) 2-3.Gram-reaction+.Gemma: ellipse, middle life.Catalase+.Catalase+.Anaerobic growth-.The V.P reaction+.Growth temperature (℃) 5-50.7% NaCl growth.Glucose, wood sugar produce acid.Pectinose, N.F,USP MANNITOL produce acid.Hydrolyzed starch not.Utilize Citrate trianion.Do not reduce nitrate.Decompose casein.Not hydrolysis tyrosine.TW-6 bacillus pumilus (bacillus pumilus) is decomposed greengrocery, meat dish class in 24 hours substantially, and is to decompose chitin, more obvious with other bacterial classification result of use.
Below be bacstearothermophilus (bacillus stearothermophilus) 1) formal name used at school;
Bacstearothermophilus (bacillus stearothermophilus) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) bacstearothermophilus (bacillus stearothermophilus) 3) other title (popular name, strain name, kind name);
TW-7 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 0.6-1.0, long (micron) 2-3.Gram-reaction+.Gemma: ellipse, end is given birth to.Catalase+.Catalase+.Anaerobic growth+.The V.P reaction-.Growth temperature (℃) 30-75.5% NaCl growth.Glucose, wood sugar, pectinose, N.F,USP MANNITOL produce acid.Utilize not aerogenesis of glucose.Hydrolyzed starch.Do not utilize Citrate trianion.Nitrate reduction.Decompose casein.Not hydrolysis tyrosine.TW-7 bacstearothermophilus (bacillus stearothermophilus) is decomposed greengrocery, meat dish class in 24 hours substantially, and is to decompose particularly fats of meat dish class, more obvious with other bacterial classification result of use.
Below be subtilis (bacillus subtilis) 1) formal name used at school;
Subtilis (bacillus subtilis) 2) classification;
Bacillaceae (Bacillaceae) bacillus (bacillus) subtilis (bacillus subtilis) 3) other title (popular name, strain name, kind name);
TW-8 (TW is the phonetic alphabet abbreviation of day luxuriant Bioisystech Co., Ltd, and numeral is numbering) 4) strain properties is described;
Thalline: wide (micron) 0.7-0.8, long (micron) 2-3.Gram-reaction+.Gemma: ellipse, middle life.Catalase+.Catalase+.Anaerobic growth-.The V.P reaction+.Growth temperature (℃) 5-55.7% NaCl growth.Glucose, wood sugar produce acid.Pectinose, N.F,USP MANNITOL produce acid.Hydrolyzed starch.Utilize Citrate trianion.Nitrate reduction.Decompose casein.Not hydrolysis tyrosine.TW-8 subtilis (bacillus subtilis) is decomposed greengrocery, meat dish class in 20 hours substantially, and is to decompose meat dish class and carbohydrate, more obvious with other bacterial classification result of use.
Above-mentioned characteristic description to each bacterial classification is selected from following document:
1. uncle's Jie Shi Bacteria Identification handbook (the 8th edition) (Bergey ' s Manual of DeterminativeBacteriology).
2. uncle's Jie Shi systematic bacteriology handbook (new edition) first roll (Bergey ' s Manual ofSystematic Bacteriology, Vol.1.1984).Bacterial classification is through continuous passage and simultaneous test, and the bacterial classification that promptly goes down to posterity (inclined-plane passed for 30 generations) with set out bacterial classification and its taxonomy proterties of type culture and physicochemical character visible change (consistence) does not take place all, illustrates that its inherited character is stable.
Effective bacterial classification of the present invention is to adopt following method separation and Culture:
1. the source of bacterial classification:
TW-1-TW-8 is distributed widely in the soil, (as temperature, humidity, nutritive deficiency) can form the gemma of dormant state when culture condition and natural environmental condition are not suitable for, the comparatively high temps short period of time stimulate or suitable nutrition, temperature, humidity condition under, gemma is sprouted into nourishing body by dormant state.Different geographical ecotopes has bigger difference as the quantity and the comfort zone of bacterial classification in lean soil, vegetable garden soil, barnyard manure, the geothermal area soil etc.General lean soil bacterium amount is few, and the temperature tolerance of bacterial classification is higher in barnyard manure and the geothermal area soil.Winter, the bacterium amount obviously was less than other season.
2. the separation condition of each bacterial classification and separation method
The TW-1-TW-8 bacterial classification is by separating in the nature soil.
Scalp about 2-3 centimetre in table soil when method 1. is separated earlier, gather soil again, gather the soil of 5 points in one about 500 square centimeters the zone, pack into after soil sample is mixed and adopt in the handmade paper bag, and indicate and gather date (summary) and place (summary).Soil is air-dry, porphyrize.Sample behind the porphyrize is spread on the aseptic kraft paper, is coated with the even hypsokinesis in shop and goes unnecessary sample particle, will contain the sample scraps of paper again at substratum (glucose 1%, extractum carnis 1%, peptone 1%, NaCl 0.5%, agar 2%, PH7.2 sterilized 25 minutes down for 121 ℃.) go up and to flick its back side with finger, being positioned over 45 ℃ of thermostat containers then and cultivating 24-72 hour, institute's bacterium colony that obtains divides pure back acquisition pure bacterium colony through repeatedly diluting method of scoring.It is stand-by to be inoculated in slant medium (it is the same to fill a prescription).
Scalp about 2-3 centimetre in table soil when method 2. is separated earlier, gather soil again, gather the soil of 5 points in one about 500 square centimeters the zone, pack into after soil sample is mixed and adopt in the handmade paper bag, and indicate and gather date (summary) and place (summary).Soil is air-dry, porphyrize.Sample is placed (glucose 1%, extractum carnis 1%, peptone 1%, NaCl 0.5%, PH7.2,121 ℃ of down sterilizations 25 minutes) in the nutrient solution 45 ℃ cultivated 24 hours, put room temperature and be placed on after 3 days in 100 ℃ of boiling water 3-5 minute, cultivate through 45 ℃ of thermostat containers with plate streak or plate isolation dilution method and to obtain single bacterium colony in 24-72 hour, institute's bacterium colony that obtains divides pure back acquisition pure bacterium colony through repeatedly diluting method of scoring.It is stand-by to be inoculated in slant medium (it is the same to fill a prescription).
3. the actual conditions during each culture of strains
Substratum: peptone (polypeptone): 1% extractum carnis: 0.5% NaCl:0.5% oral glucose: 1% agar powder: 1.7% PH:7.20.1Mpa sterilization 25 minutes.Cultivate with (TW1-TW8) for strain inclined plane.Liquid nutrient medium: peptone (polypeptone): 1% extractum carnis: 0.5% tryptone: 1%K
2HPO
43H
2O:0.2% (NH
4)
2SO
4: 1.5% NaCl:0.5%MgSO
47H
2O:0.07% oral glucose: 1% corn steep liquor 1.5%PH7.2 0.1Mpa, 25 minutes (TW1-TW8) that sterilize.Liquid nutrient medium is used for cultivating in a large number bacterial classification.
All bacterial classifications are mixed culture not all.
4. the mixing match between each bacterial classification:
Because also all differences of contained organic content so the proportioning between each bacterial classification is indefinite, but change to some extent according to different inclusion, thereby reach best effect in the different biologic garbages.As the domestic organic garbage thrown in based on starch and vegetables, decomposing this moment must breed more based on the bacterial classification of starch and vegetables, and more when the domestic organic garbage of throwing in meat (or being called meat dish), decompose this moment and can in system, preponderate again based on the bacterial classification of protein and lipid.In other words, because the content difference of domestic organic garbage, the quantity of each bacterial classification is dynamic in system.
The bacterium quantitative proportion that contains between each bacterial classification is: bacillus megaterium (Bacillus megetarium): Bacillus cercus (Bacillus cereus): bacillus laterosporus (Bacilluslaterosporus): bacillus licheniformis (Bacillus licheniformis): bacillus thuringiensis (Bacillus thuringiensis): bacillus pumilus (bacillus pumilus): bacillus stearothermophilus (bacillus stearothermophilus): bacillus subtilis (bacillus subtilis)=0.1-2.5: 0.5-1.5: 0.1-1.5: 0.1-2.0: 0.1-3.0: 0.1-2: 0.1-2.5: 0.1-2.5
Proportioning commonly used is: 0.7-1.7: 0.5-1.5: 0.2-1.2: 0.1-1.1: 0.8-1.8: 0.5-1.5: 0.6-1.6: 0.6-1.6.
Best proportioning between each bacterial classification is: bacillus megaterium (Bacillus megetarium): Bacillus cercus (Bacillus cereus): bacillus laterosporus (Bacillus laterosporus): bacillus licheniformis (Bacillus licheniformis): bacillus thuringiensis (Bacillusthuringiensis): bacillus pumilus (bacillus pumilus): bacillus stearothermophilus (bacillus stearothermophilus): bacillus subtilis (bacillus subtilis)=1.2: 1.0: 0.7: 0.6: 1.3: 1.0: 1.1: 1.1.
Microbial strains is decomposed the technological line of domestic organic garbage:
The combination of screening of microbial strains → obtain effective bacterial classification → slant culture → shake-flask culture → fermentor tank (enlarged culturing) → bacterial classification → and strain substrate drop into domestic organic garbage handler (in the domestic organic garbage handler, decomposing domestic organic garbage) → resistates → organic fertilizer (going back native soil) together.
The biological organic garbage handler mainly is by alr mode organic waste effectively to be mixed with bacterial classification, improves the efficient of decomposing rubbish.
Bacterial classification is joined in the microorganism organic garbage disposal of strain substrate (bacterium bed), drop into domestic organic garbage again, begin after a few hours to decompose, 12-20 hour basic the decomposition fully.Decompose the back overwhelming majority and discharge as gas, elimination factor can reach more than 95%.And under low temperature, middle temperature, pyritous condition, all can decompose organic waste with fast speeds.Bio-matrix in organic garbage disposal effectively the time length be 2-6 month, cleaning in promptly 2-6 month is (during can add a small amount of bacterial classification as required) once.
Effectively bacterial classification can produce proteolytic enzyme, lipase, amylase, chitin enzyme (chitinase), cellulase, oxydase etc. respectively, thereby make macromolecular substance (main component of domestic organic garbage) be become lower-molecular substance by microbiological degradation, behind these lower-molecular substances of microorganism panning, be converted into CO
2, H
2O and minor N H
3(and releasing energy) discharged with the form of gas and steam, and effectively eliminated odor pollution, makes domestic organic garbage (solid-state form) reach decrement.
The major ingredient of domestic organic garbage---macromolecular substance is become lower-molecular substance by microbiological degradation, behind these lower-molecular substances of microorganism panning, in 12-24 hour it 95% is transformed into CO
2, H
2O, small amount of N H
3And energy, discharge main frame with gas and water vapor form and enter spray equipment.Through water spray system to CO
2, NH
3Absorption and filtration, make emission gases reach national specified discharge standard.The material that domestic organic garbage remainder 5% is not decomposed (with the dust shape) mixes with bio-matrix.Bacterial classification decomposes the principle and the characteristics of domestic organic garbage:
(1) principle:
Effectively bacterial classification can produce proteolytic enzyme, lipase, amylase, chitin enzyme (chitinase), cellulase, oxydase etc. respectively, thereby make macromolecular substance (main component of domestic organic garbage) be become lower-molecular substance by microbiological degradation, behind these lower-molecular substances of microorganism panning, be converted into CO
2, H
2O and minor N H
3(and releasing energy) discharged with the form of gas and steam, and effectively eliminated odor pollution, makes domestic organic garbage (solid-state form) reach decrement.
(2) bacterial classification characteristics:
A. eight kinds of biologically active substances that effective microbe is composited after pure culture, bacteria containing amount 〉=1.0-
2.0 * 10
11Individual/gram.
B. definite through checking, to humans and animals safety, harmless, nontoxic, no teratogenecity.(acute oral
Toxicity test LD
50＞5g/kg; The Ames method detects negative).
C. temperature range big (15-70 ℃ of scope), i.e. equal energy under low temperature, middle temperature, hot conditions
Decompose domestic organic garbage.
D. some intractable waste there is stronger capacity of decomposition.
E.PH (4-9) wide accommodation, domestic organic garbage promptly acid and alkalescence all can be decomposed.
F. the salts substances of high density (0.7M) does not influence its capacity of decomposition.
G. the stabilization characteristics of genetics of bacterial classification.
(3) microbial strains special case
The ammonification of TW-1 bacterial classification tool.TW-2 bacterial classification tool decomposes the organo phosphorous compounds effect.TW-4 bacterial classification tool deamination.The TW-5 bacterial classification forms parasporal crystal in the cell at later stages.The TW-6 bacterial classification decomposes chitin.
The present invention by separate at nature, screening obtained one group and had the microorganism (wherein some bacterium is the organic degradation bacteria of intractable) of decomposing all kinds of domestic organic garbage abilities, can effectively decompose the waste of the chicken and duck flesh of fish, shrimp and crab shells, eggshell, vegetables skin, shell, melon and fruit peel, hogwash or the like.Study by the characteristics life history of above effective microbe being carried out growth temperature, oxygen consumption demand, salt tolerance, PH subject range, nutritional requirement etc. and bacterial classification again, after organic assembling, just obtained the microorganism species of domestic organic garbage " elimination type ".
The present invention is further illustrated below by embodiment.
Embodiment 1:
When the composition of domestic organic garbage mainly was protein, the ratio between each bacterial classification was
TW1∶TW2∶TW3∶TW4∶TW5∶TW6∶TW7∶TW8＝1.5∶1.0∶0.5∶1.0∶1.0∶1.0∶1.0∶1.0，
It decomposes effect: good.
Embodiment 2:
When the composition of domestic organic garbage mainly was fat, the ratio between each bacterial classification was
TW1∶TW2∶TW3∶TW4∶TW5∶TW6∶TW7∶TW8＝1.0∶1.0∶0.7∶0.4∶1.2∶1.0∶1.6∶1.1，
It decomposes effect: good.
Embodiment 3:
When the composition of domestic organic garbage mainly is carbohydrate (starch, sugar, Mierocrystalline cellulose etc.), each
Ratio between the bacterial classification is
TW1∶TW2∶TW3∶TW4∶TW5∶TW6∶TW7∶TW8＝1.2∶1.0∶0.4∶1.0∶0.6∶1.0∶1.4∶1.4，
It decomposes effect: good.
Embodiment 4:
When the composition of domestic organic garbage mainly was chitin, the ratio between each bacterial classification was
TW1∶TW2∶TW3∶TW4∶TW5∶TW6∶TW7∶TW8＝1.0∶1.0∶0.7∶0.6∶1.1∶1.6∶1.0∶1.0，
It decomposes effect: good.
Embodiment 5:
When the composition of domestic organic garbage mainly was the mixing domestic organic garbage, the ratio between each bacterial classification was
TW1∶TW2∶TW3∶TW4∶TW5∶TW6∶TW7∶TW8＝1.2∶1.0∶0.7∶0.6∶1.3∶1.0∶1.1∶1.1，
It decomposes effect: good.
The microorganism organic garbage disposal according to day treating refuse amount difference different models is arranged respectively, but its principle is identical with structure.This machine divides main frame and annex two partly.
Partly for loading casing (volume ratio maximum), loading the casing middle part is stir shaft, has paddle on the axle at the center of main frame, and stir shaft drives stirring system through the reducing motor traction by chain gear domestic organic garbage and bio-matrix are stirred.
Two bright wind hand-holes and an exhausting hole are arranged at the top of loading casing, rubbish is when being decomposed into steam and gas, by vacuum fan with steam and gas by force outside the shedder, two bright wind constantly flow into fresh air under the drive of vacuum fan, allow at the rubbish and the continuous ingress of air of bio-matrix that stir, thereby reach the de-odorised purpose.
Load the outer wall of casing, encased, and, regulate automatically loading spin manifold temperature according to the setting of intelligence instrument by the flexible film exothermic material.Intelligence instrument also can be provided with and control automatically stirring system and exhausting system except that temperature is regulated automatically simultaneously.
Annex is a water spray system.Extract steam and gas out through vacuum fan, after the water spray system spray absorbs and filters, enter water drain through pipeline again.
The operational process of organic garbage disposal:
1, open the main frame case lid, (its weight ratio is 80-120: 1: 40-60), the stirring system of machining makes wood chip, bacterial classification and water uniform distribution to drop into bio-matrix (wood chip), domestic organic garbage decomposition agent (bacterial classification) and water.
2, according to the quantitative domestic organic garbage of different type of machines input, (stirring is set at: stirred 2-3 minute, stopped 20 minutes to start stirring system, exhausting system and heating system; Exhausting is set air quantity: 6-8 rice
3/ minute, temperature is set in 40 ℃-45 ℃).
3, under the condition that machine is set, the major ingredient of domestic organic garbage---macromolecular substance is become lower-molecular substance by microbiological degradation, behind these lower-molecular substances of microorganism panning, in 12-24 hour it 95% is transformed into CO
2, H
2O, small amount of N H
3And energy, discharge main frame with gas and water vapor form and enter water spray system.Through water spray system to CO
2, NH
3Absorption and filtration, make emission gases reach national specified discharge standard.The material that domestic organic garbage remainder 5% is not decomposed (with the dust shape) is mixed with bio-matrix.
4, can drop into quantitative domestic organic garbage by said procedure every day.
5, the complete machine operation is after 4-6 month, and life matrix also is decomposed.When life matrix near pasty state, when garbage decomposition speed is slack-off, need to change bio-matrix and domestic organic garbage decomposition agent.The resistates that is replaced out can be done fertilizer and be used for arable farming.
Effect data when mixed bacterial classification decomposes various rubbish under the situation of embodiment 5.
The ratio of mixture of its bacterial classification is:
TW1∶TW2∶TW3∶TW4∶TW5∶TW6∶TW7∶TW8＝1.2∶1.0∶0.7∶0.6∶1.3∶1.0∶1.1∶1.1。
The test-results of embodiment 5
*The input amount G0=60 kilogram at microbial bacteria and matrix initial stage
*The resistates total amount G1=807 kilogram that takes out after the off-test
*The organic waste total amount G=15000 kilogram elimination factor (total amount reduction rate) that duration of test drops into is:
WR=[1-(G1-G0)/G] * 100%=[1-(807-60)/15000] * 1000%
| Time (my god) | Rubbish is formed (kilogram) | Input amount (kilogram) | Domestic organic garbage is formed classification | Effect |
| Amount to 150 days | Greengrocery | About 6000 | Green vegetables, Caulis et Folium Brassicae capitatae, celery, leek, Peel of Chinese Waxgourd, tomato, mushroom root, the old root of garlic sprouts, sweet potato, shepherd's purse, Radix Dauci Sativae, lotus root section, romaine lettuce, black bamboo shoot, bean seedlings, bean sprouts root etc. | Well |
| Animal, fish viscera and chicken giblets | About 4000 | Pluck, fish head, fish tail, eel bone, pig lung, sepia bone plate, pig bone, pork skin, chicken head, chicken and duck tail etc. | Well | |
| The fruit resistates | About 800 | Apple pear skin, nuclear, orange peel, orange peel, shaddock skin, Pericarpium Musae, pineapple peel, Watermelon rind etc. | Well | |
| Prepared food | About 3600 | The chicken and duck flesh of fish that wheaten food, rice food, steamed bun, cruller, fry are fried etc. | Well | |
| Other | About 600 | Ovum crusta Gallus domesticus, potato, sweet potato, crab shell, crab pin, bamboo shoot shell, shrimp shell etc. | Well | |
| Amount to | About 15000 | 807 kilograms of resistatess (comprising matrix etc.) |
≈95％
Claims (7)
1. handle the bacterial classification of domestic organic garbage, it is by following bacterial classification mixture according to a certain ratio: bacillus megaterium (Bacillus megetarium), bacillus cereus (Bacillus cereus), bacillus laterosporus (Bacillus laterosporus), Bacillus licheniformis (Bacilluslicheniformis), bacillus thuringiensis (Bacillus thuringiensis), bacillus pumilus (bacillus pumilus), bacstearothermophilus (bacillusstearothermophilus), subtilis (bacillus subtilis), its proportioning in order is 0.1-2.5: 0.5-1.5: 0.1-1.5: 0.1-2.0: 0.1-3: 0.1-2: 0.1-2.5: 0.1-2.5, eight kinds of biologically active substances that effective microbe is composited after pure culture, bacteria containing amount 〉=1.0-2.0 * 10
11Individual/gram, face when using and mix.
2. the bacterial classification of processing domestic organic garbage according to claim 1, it is characterized in that: its substratum is: peptone: 1% extractum carnis: 0.5% NaCl:0.5% oral glucose: 1% agar powder: 1.7% PH:7.20.1Mpa sterilization 25 minutes, cultivate usefulness for strain inclined plane, all bacterial classifications are mixed culture not all.
3. the bacterial classification of processing domestic organic garbage according to claim 1 is characterized in that: its liquid nutrient medium is: peptone: 1% extractum carnis: 0.5% tryptone: 1%K
2HPO
43H
2O:0.2% (NH
4)
2SO
4: 1.5% NaCl:0.5%MgSO
47H
2The O:0.07% oral glucose: 1% corn steep liquor 1.5%PH7.2 0.1Mpa, to sterilize 25 minutes, all bacterial classifications are mixed culture not all.
4. the bacterial classification of processing domestic organic garbage according to claim 1 is characterized in that: the proportioning between each bacterial classification is: 0.7-1.7: 0.5-1.5: 0.2-1.2: 0.1-1.1: 0.8-1.8: 0.5-1.5: 0.6-1.6: 0.6-1.6.
5. the bacterial classification of processing domestic organic garbage according to claim 1 is characterized in that: the proportioning between each bacterial classification is: 1.2: 1.0: 0.7: 0.6: 1.3: 1.0: 1.1: 1.1.
6. according to the bacterial classification of the described processing domestic organic garbage of claim 1, it is characterized in that: the optimal temperature scope all can be decomposed domestic organic garbage at 15-70 ℃.
7. the bacterial classification of processing domestic organic garbage according to claim 1, it is characterized in that: the investing method of bacterial classification is: bacterial classification is dropped into bio-matrix wood chip and water, its part by weight is 1: 80-120: 40-60, stirring makes wood chip, bacterial classification and water uniform distribution, puts into organic waste again and decomposes.
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| CN100439491C (en) * | 2006-05-19 | 2008-12-03 | 中国石油化工股份有限公司 | Globular bacillus and its use |
| ES2328758A1 (en) * | 2005-08-09 | 2009-11-17 | Entorn Quimic, S.L. | Accelerating enzymatic bacteriological compound of the decomposition of the organic matter. (Machine-translation by Google Translate, not legally binding) |
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| CN100413541C (en) * | 2005-12-19 | 2008-08-27 | 西北农林科技大学 | Agricultural waste deodorizing bacterial prepn and its production process |
| CN100439491C (en) * | 2006-05-19 | 2008-12-03 | 中国石油化工股份有限公司 | Globular bacillus and its use |
| CN100383235C (en) * | 2006-05-31 | 2008-04-23 | 北京嘉博文生物科技有限公司 | Method for resource circulating treatment of dining-kitchen organic waste using compound bacteria |
| CN101941851A (en) * | 2010-08-31 | 2011-01-12 | 北京嘉博文生物科技有限公司 | Technology and process for preparing biochemical humic acid by using kitchen waste |
| CN101941851B (en) * | 2010-08-31 | 2012-12-26 | 北京嘉博文生物科技有限公司 | Technology and process for preparing biochemical humic acid by using kitchen waste |
| CN102010843A (en) * | 2010-09-02 | 2011-04-13 | 浙江大学 | Food waste degradation and elimination type microbial bacterial agent, preparation method thereof and bacteria used thereby |
| CN102010843B (en) * | 2010-09-02 | 2011-12-28 | 浙江大学 | Food waste degradation and elimination type microbial bacterial agent, preparation method thereof and bacteria used thereby |
| CN102925357B (en) * | 2011-08-10 | 2015-02-04 | 无锡中泉生物科技有限公司 | Composite microbial inoculum for harmless processing, and its application |
| CN102925357A (en) * | 2011-08-10 | 2013-02-13 | 无锡中泉生物科技有限公司 | Composite microbial inoculum for harmless processing, and its application |
| CN103159511A (en) * | 2011-12-16 | 2013-06-19 | 河北农业大学 | Bacillus thuringiensis solid fermentation process and application of same in agriculture |
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| CN104307841A (en) * | 2014-08-29 | 2015-01-28 | 广东源盛生态环保科技有限公司 | Low-cost method for killing domestic garbage infectious disease source |
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