CN100439491C - Globular bacillus and its use - Google Patents
Globular bacillus and its use Download PDFInfo
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- CN100439491C CN100439491C CNB200610046644XA CN200610046644A CN100439491C CN 100439491 C CN100439491 C CN 100439491C CN B200610046644X A CNB200610046644X A CN B200610046644XA CN 200610046644 A CN200610046644 A CN 200610046644A CN 100439491 C CN100439491 C CN 100439491C
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- sphaericus
- bacterial strain
- bacillus
- dimethylbenzene
- water
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The Staphylococcus xylous 2J1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1486. It has excellent efficient degradable performance for dimethyl benzene and can be used to dispose waste water and waste gas containing dimethyl benzene, polluted water and soil.
Description
Technical field
The invention belongs to biological technical field, relate in particular to a globular bacillus and application thereof.
Background technology
Oil and various oil fuel excessive spills and the seepage of leakage and underground oil storage facility causes underground water to suffer the pollution of aromatic hydrocarbon, and be very serious in the world.Wherein mononuclear aromatics pollutents such as benzene, toluene and dimethylbenzene are paid close attention to by people most, they all are water-soluble best compounds in oil and the various oil fuel, have constituted organic pollutant the most common in the underground water.And they belong to volatile organic compounds, have become a big class atmospheric polluting material that is only second to particulate pollutant, and it is to atmospheric seriously polluted, and is very harmful.As important chemical material, the waste gas waste water that production plants such as organic synthesis, rubber is synthetic, fiber is synthetic, paint, dyestuff, refining of petroleum, pharmacy, Mierocrystalline cellulose produce, and the discharging in the use are the main sources of dimethylbenzene in the environment.Its volatilization gas diffuses in the face of land, current and the settling after entering atmospheric layer, brings very big harm for human body and environment.Dimethylbenzene not only has hormesis to human body, and the human central nervous system is had the acute hazard effect.Thereby the improvement xylene pollution has become an instant job.
Administering in the organic contamination, Production by Catalytic Combustion Process and extraction facture are generally adopted in the past, but these methods are not only consuming time but also expense is extremely expensive.The biological treating technology can be utilized the microbiological deterioration organic pollutant in the physical environment, can implement original position and administer, and, technical feasibility simple with its equipment, cost is cheap relatively and non-secondary pollution and generally being accepted by people, has development prospect widely.Aspect the Pollution abatement of benzene compounds, the biological treating technology is being brought into play more and more important effect.
Through years of development, biotechnology has obtained significant progress in environmental improvement, developed many new technologies, and wherein biological reinforcing technology is a hot technology.Biological reinforcing technology is exactly in order to improve the processing power of biological treatment system, in this system, add from the dominant bacteria of occurring in nature screening or the high-efficiency strain that produces by assortment of genes technology, remove a certain or a certain class objectionable impurities, with of the reaction of enhanced biological amount to a certain specific environment or specific pollutant.The key condition of implementing biological reinforcing technology is the efficient degrading bacteria that obtains at target contaminant.
Therefore, but center on screening some more valuable specificitys ground degraded dimethylbenzene and similar organic bacterial strains thereof, and be applied to the biological treating process of xylene pollution, always be the problem that this area need solve.ES2085239 discloses a strain pseudomonas (CECT 4501) and has been used for the biodegradable method of aromatic hydrocarbons, and the deficiency of this microorganism is that the selectivity of its p-Xylol is good inadequately.
Summary of the invention
The purpose of this invention is to provide the bacterial isolates and the application thereof of strain degraded dimethylbenzene.
Bacterial strain provided by the invention is Bacillus sphaericus (Bacillus sphaericus) 2J1, be designated hereinafter simply as B.sphaericus 2J1, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC) on October 10th, 2005, deposit number is CGMCC No.1486.
The physiological and biochemical property of this B.sphaericus 2J1 bacterial strain sees Table 1.
The physiological and biochemical property of table 1 B.sphaericus 2J1 bacterial strain
B.sphaericus 2J1 bacterial strain of the present invention can be simple inorganics (carbonic acid gas, water) and cell integral part through Degradation and Transformation with only carbon source and the energy of dimethylbenzene as growth and breeding.This B.sphaericus 2J1 bacterial strain can tolerate the dimethylbenzene of high density, and p-Xylol has efficient single-minded degradation property, thereby can be widely used in the biological treating of xylene pollution, especially be fit to handle the high density pollution that causes as sudden situations such as leakages, thereby can the enhanced biological treatment technology, improve processing efficiency, save the treatment time.
Biomaterial preservation explanation
B.sphaericus 2J1 bacterial strain provided by the invention is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC); Address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City Institute of Microorganism, Academia Sinica; Deposit number: CGMCC No.1486; Preservation date: on October 10th, 2005.
Embodiment
Specify the screening process and the effect of microorganism strains of the present invention below.
Separation and the cultivation of embodiment 1, Bacillus sphaericus B.sphaericus 2J1
1, the separation of bacterial strain
Gather mud sample in sewage work of Fushun No.3 Petroleum Factory.Get 1 sludge water solution on screening culture medium, add 0.1g dimethylbenzene, smear evenly, be placed in the incubator, cultivated 2 days for 30 ℃ with spreader; With transfering loop the bacterial classification that screens is forwarded to the LB substratum then, places incubator to cultivate 1 day for 30 ℃, the gained bacterial classification is the bacterial classification of degraded dimethylbenzene.
Consisting of of described screening culture medium:
(NH
4)
2SO
4 5g； K
2HPO
4 1g；
KH
2PO
4 4g； NaCl 2g；
MgSO
47H
2O 1g; Agar 15g;
Water 1000mL; PH 7.0.
2, the cultivation of bacterial strain
With transfering loop the bacterial classification that filters out is connected to and shakes in the bottle, liquid amount is 50mL, cultivates in the shaking culture case.Used substratum is that LB substratum or other natural medium all can; Culture condition is: 30 ℃ of temperature; Rotating speed 150 commentaries on classics/min; Incubation time 20h.When cell growth reaches logarithmic growth latter stage, the dense maximum value that reaches of bacterium is through centrifugal or filter and can obtain microbial inoculum.This cell can directly be used, or it is stand-by to be placed on 4 ℃ of refrigerator preservations.
The component of described LB substratum is:
Yeast extract paste extract 5g; Peptone 10g;
NaCl 10g; Agar 15g;
Water 1000mL; PH 7.0.
Therefore the B.sphaericus 2J1 bacterial strain of the present invention dimethylbenzene of can degrading under aerobic condition can be applicable to contain waste water, waste gas, the water of xylene pollution and the biological treating process of soil of dimethylbenzene.
Following embodiment specifies the effect of bacterial strain of the present invention.
Embodiment 2
The cultivation of B.sphaericus 2J1 bacterial strain is identical with embodiment 1.Get 2 and shake bottle, add 50mL sterilized water and 1mL toluene respectively.Get one of them and shake bottle as the blank group, another shakes bottle and adds cultured 2J1 microbial inoculum, and making its OD value is 4.2.Shake bottle with two and be placed on oscillatory reaction on the shaking table, the shaking table condition is: 30 ℃ of temperature; Rotating speed 150 commentaries on classics/min.Sampling analysis behind the 10h, the degradation results of dimethylbenzene sees Table 2.
Table 2 B.sphaericus 2J1 bacterial strain is handled the result who contains toluene sewage
Embodiment 3
The cultivation of B.sphaericus 2J1 bacterial strain is identical with embodiment 1.Get 2 and shake bottle, adorn the 50mL sterilized water respectively, add 4g again by the soil of 1mL xylene pollution.Get one and shake bottle as the blank group; Another shakes bottle and adds cultured B.sphaericus 2J1 microbial inoculum, and making its OD value is 5.0.Shake bottle with 2 and be placed on oscillatory reaction on the shaking table.The shaking table condition is: 30 ℃ of temperature; Rotating speed 150 commentaries on classics/min.Sampling analysis behind the 10h the results are shown in table 3.
Table 3 2J1 bacterial strain is handled the result of xylene pollution soil
Embodiment 4
The cultivation of B.sphaericus 2J1 bacterial strain is identical with embodiment 1.Get 1 and shake bottle, add the 1L sterilized water, add cultured 2J1 microbial inoculum again, making its OD value is 5.0.Add about 150mL slag toward shaking in the bottle, will shake and bottle be placed on the shaking table about shaking cultured cell 4h.The shaking table condition is: 30 ℃ of temperature; Rotating speed 150 commentaries on classics/min.Take out slag after treating on the slag to generate the mycoderm of capacity, (among the φ 2cm * H50cm), be that 30 ℃, inlet xylene concentration are 100.0mg/L in temperature, airshed is under the operational condition of 80L/h to the packing tower of packing into, and the average degradation rate of dimethylbenzene is 85.94%.
The result of embodiment 2-4 shows that B.sphaericus 2J1 bacterial strain p-Xylol of the present invention has the efficient degradation performance, can be widely used in the biological treating of xylene pollution.
Claims (2)
1, a globular bacillus (Bacillus sphaericus) 2J1, CGMCC No.1486.
2, the application of the described bacterial strain of claim 1 in containing xylene emission, waste water and soil remediation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200610046644XA CN100439491C (en) | 2006-05-19 | 2006-05-19 | Globular bacillus and its use |
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|---|---|---|---|
| CNB200610046644XA CN100439491C (en) | 2006-05-19 | 2006-05-19 | Globular bacillus and its use |
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| CN101074423A CN101074423A (en) | 2007-11-21 |
| CN100439491C true CN100439491C (en) | 2008-12-03 |
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| CNB200610046644XA Active CN100439491C (en) | 2006-05-19 | 2006-05-19 | Globular bacillus and its use |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432111B (en) * | 2011-11-24 | 2014-03-05 | 复旦大学 | Method for enhancing petroleum pollution removing effect of seawater |
| CN103333827A (en) * | 2013-06-25 | 2013-10-02 | 软控股份有限公司 | Method for screening and separating strains for treating rubber waste gas |
| CN106477731A (en) * | 2016-10-18 | 2017-03-08 | 桂林洁宇环保科技有限责任公司 | Application of the anaerobic spore-bearing bacilli in Hospital Sewage Treatment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6057268A (en) * | 1997-12-12 | 2000-05-02 | Organica, Inc. | Solid form compositions for treating natural bodies of water |
| JP2001299331A (en) * | 2000-04-19 | 2001-10-30 | Nakatani Takanari | Refuse treating medium, method for producing refuse treating medium, method for treating refuse and refuse treating machine |
| CN1370829A (en) * | 2001-02-27 | 2002-09-25 | 广州天蔚生物技术有限公司 | Bacterial spawn for treating domestic organic garbage |
-
2006
- 2006-05-19 CN CNB200610046644XA patent/CN100439491C/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6057268A (en) * | 1997-12-12 | 2000-05-02 | Organica, Inc. | Solid form compositions for treating natural bodies of water |
| JP2001299331A (en) * | 2000-04-19 | 2001-10-30 | Nakatani Takanari | Refuse treating medium, method for producing refuse treating medium, method for treating refuse and refuse treating machine |
| CN1370829A (en) * | 2001-02-27 | 2002-09-25 | 广州天蔚生物技术有限公司 | Bacterial spawn for treating domestic organic garbage |
Non-Patent Citations (2)
| Title |
|---|
| 生物过滤塔甲苯降解性能研究. 李国文等.环境科学，第22卷第2期. 2001 |
| 生物过滤塔甲苯降解性能研究. 李国文等.环境科学，第22卷第2期. 2001 * |
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| CN101074423A (en) | 2007-11-21 |
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