CN101643707B - Microbial inoculum for degrading polycyclic aromatic hydrocarbons - Google Patents
Microbial inoculum for degrading polycyclic aromatic hydrocarbons Download PDFInfo
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- CN101643707B CN101643707B CN2008101180773A CN200810118077A CN101643707B CN 101643707 B CN101643707 B CN 101643707B CN 2008101180773 A CN2008101180773 A CN 2008101180773A CN 200810118077 A CN200810118077 A CN 200810118077A CN 101643707 B CN101643707 B CN 101643707B
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Abstract
The invention provides a microbial inoculum for degrading polycyclic aromatic hydrocarbons, which consists of two strains of light yellow mycobacterium W52 and light yellow mycobacterium M16. The microbial inoculum has the capability of degrading various polycyclic aromatic hydrocarbons, is suitable for paddy soil, vegetable garden soil and red soil in the South, moisture soil in the North and other types of soil, has remarkable effect of remediating the soil contaminated by the polycyclic aromatic hydrocarbons, and simultaneously can facilitate the growth of plants, improve the crop yield, degrade over 98 percent of tricyclic (fluorene, naphthalene, anthracene, acenaphthylene, phenanthrene) polycyclic aromatic hydrocarbons and degrade over 90 percent of tetracyclic pyrene.
Description
Technical field
The invention belongs to the microbiobacterial agent field, particularly, relate to a kind of microbial inoculum for degrading polycyclic aromatic hydrocarbons that contains pale yellow mycobacterium.
Background technology
(Polycyclic Aromatic Hydrocarbons PAHs) is meant the hydrocarbon polymer that contains two or more phenyl ring in the molecule to polycyclic aromatic hydrocarbons, can be divided into aromatic condensed ring type and fragrant non-condensed ring type.The aromatic condensed ring type is meant that phenyl ring adjacent in the molecule has the hydrocarbon polymer of two shared carbon atoms at least, as naphthalene, anthracene, phenanthrene and pyrene etc.
Polycyclic aromatic hydrocarbons is the very strong environmental pollutant of a class carinogenicity, is one of most important monitoring project in the environmental pollution.Along with coal, the widespread use of oil in industrial and agricultural production, communications and transportation and life, the polycyclic aromatic hydrocarbons of Chan Shenging has become the common organic pollutant of paying close attention in countries in the world therefrom, and the accumulation of polycyclic aromatic hydrocarbons has more and more seriously threatened human health.
Along with the fast development of domestic industry and being extensive use of of fossil oil, domestic many areas have suffered the severe contamination (Ge Chengjun etc., 2005) of polycyclic aromatic hydrocarbons.Because PAHs generally has three causing property, biological accumulation, long-distance migration and half volatile and can exist lastingly in environment, and is put into typical persistence organic pollutant (POPs) ranks, and is subjected to extensive concern (Bairdet al., 2005; Wang Peihua, 2006; Erickson et al., 1999).Pyrene is the high molecular PAHs with four phenyl ring of typical difficult degradation, be prevalent in the environment, and some derivatives of pyrene has stronger toxicity.
Microbiological deterioration PAHs is owing to have cheapness, thoroughly and the advantage of non-secondary pollution and the extremely attention of researcher and concern (Wilson and Jones, 1993; Cheng Guoling etc. 2003).As far back as nineteen twenty-eight, Tausson just separates the assimilation bacteriums that obtained three ring PAHs anthracenes and phenanthrene from the oil immersion pedotheque in oil field, Baku, and afterwards, the scientific research personnel finds the microorganism of many harmonic component PAHs that can degrade again successively.Because Fourth Ring PAHs molecular weight is difficult for greatly being degraded by microorganisms, the difficulty of obtaining of Fourth Ring PAHs degradation bacteria also increases thereupon.Over nearly twenties years, the bacterial strain of can degrade Fourth Ring PAHs pyrene and fluoranthene even five rings PAHs benzo [a] pyrene is constantly reported.Wherein, the plant division bacillus is the very important bacterium for degrading of a class.Mycobacteriumvanbaalenii PYR-1 is mycobacterium (Hetikamp, 1988 of first strain degraded pyrene; Heitkamp et al., 1988).Afterwards, the researchist finds many different mycobacteriums again, as Mycobacterium sp.strain BB1 (Boldrin et al, 1993), Mycobacterium sp.strain RJGII-135 (Schneider et al., 1996), Mycobaterium sp.strain KR20 (Rehmann et al, 1998), Mycobaterium sp.strain AP1 (Vila et al., 2001) and Mycobaterium sp.strain JLS (Miller et al, 2004), Mycobacteriumflavescens (Dean-Ross, 1996) etc.U.S. Cerniglia laboratory is the most deep to the research of Mycobacterium vanbaalenii PYR-1, has finished complete genomic examining order (http://imgjgi.doe.gov); By biology approach, complete pathways metabolism (Kim et al, 2006 of pyrene and fluoranthene have been taken the lead in cracking; Kweon et al., 2007).
Natural microorganism is generally slower to the polycyclic aromatic hydrocarbon pollution repair process, be difficult to practical application, thereby under artificial promotion condition, utilize the Degradation of inoculation inoculating microbe, remove the microorganism recovery technique of polycyclic aromatic hydrocarbons in the soil, improve the speed of degrading polycyclic aromatic hydrocarbons, and plant and microbial association reparation have become the research focus in soil remediation field.
The seventies in 20th century, the researchist carried out trial (the Ahmed and Focht that utilizes microorganism to repair polycyclic aromatic hydrocarbon pollution, 1973), the eighties, scientist recognized that microorganism is the technology that good prospect the is arranged most (Atlas of polycyclic aromatic hydrocarbons contaminated environment remediation, 1981), the nineties in 20th century, the biological restoration development of oil and polycyclic aromatic hydrocarbons contaminated contaminate environment rapidly, utilize dissimilar bacterial strains, regulate and control optimal growth conditions, obtained the repairing effect that catches people's attention.The result of study of achromobacter Achromobacter sp. and mycobacterium Mycobacterium sp. degrading polycyclic aromatic hydrocarbons shows, nutrition supply influences degradation effect, degradation effect was the highest when Achromobacter sp. supplied at phosphoric acid salt, and mycobacterium Mycobacterium sp obtains best effect (Cutright and Lee, 1994) when chlorine-containing compound is supplied.The Canadian Studies personnel adopt microbe inoculation and machinery to stir the reparation of carrying out contaminated soil, and the soil inoculation bacterial strain reaches 10
9Cfu/g soil, the solid state repairing effect is good, and total PAHs drops to 100 μ g/g soil by 1000 μ g/g soil, and benzopyrene is dropped to less than 10 μ g/g soil (Hyzy andSchepart, 1995) by 100 μ g/g.Bewley (1991) at first using gene engineering bacterium repairs gasoline contamination ground, and the potential associated problem of biological reinforced reparation are measured, for the requirement of its superiority utilization is explored.It is generally acknowledged that white rot fungus degrading polycyclic aromatic hydrocarbons ability is strong, but studies show that, filamentous fungus has the polycyclic aromatic hydrocarbons contaminated effect of efficient degradation soil equally, the original position repairing test result of natural soils shows, the effect of inoculation mycelium degraded soil polycyclic aromatic hydrocarbons is best, but the different strains difference on effect is big (Potin and Rafin, 2004).
Along with the scientific research personnel carries out the further investigation of biological restoration to contaminated soil, find that some plant can promote microorganism to the repair in the contaminated soil.Plant is in repair process, can discharge root rhizosphere secreta and the enzyme that promotes chemical reaction, the rhizosphere effect has increased the quantity of microbiological deterioration bacterium, is because the plant secretion organism provides metabolic matrix substrate (Alley and Brown, 2000) altogether for microorganism is total to metabolism on the other hand.
In sum, the biological restoration of utilizing the degrading polycyclic aromatic hydrocarbons microorganism to carry out contaminated soil has huge application potential.Mycobacterium is an important microbe groups with Fourth Ring and the strong degradation capability of above ring PAHs, the existing report of the bacterial strain screening of mycobacterium degraded PAHs, but the microbiobacterial agent of mycobacterium and the repairing effect of polycyclic aromatic hydrocarbon pollution do not appeared in the newspapers.
Summary of the invention
The purpose of this invention is to provide a kind of microbial inoculum for degrading polycyclic aromatic hydrocarbons.
In order to realize purpose of the present invention, microbial inoculum for degrading polycyclic aromatic hydrocarbons of the present invention, its effective constituent comprises:
Pale yellow mycobacterium W52;
Pale yellow mycobacterium M16.
Wherein, pale yellow mycobacterium W52 bacterial strain of the present invention, be numbered CGMCC NO.2274, its classification called after Mycobacterium gilvum, now be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, Datun Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, deposit number CGMCC NO.2274, preservation date on November 30th, 2007.
Pale yellow mycobacterium M16 bacterial strain of the present invention, be numbered CGMCC NO.2273, its classification called after Mycobacterium gilvum, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, Datun Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, deposit number CGMCC NO.2273, preservation date November 30 in 2007.
Microbial inoculum for degrading polycyclic aromatic hydrocarbons of the present invention, it comprises the component of following weight part:
Pale yellow mycobacterium W52 1-5 part;
Pale yellow mycobacterium M16 1-5 part;
Additive 10-15 part.
Preferably:
1 part of pale yellow mycobacterium W52;
1 part of pale yellow mycobacterium M16;
12 parts of additives.
Described additive is the peat composed of rotten mosses or diatomite, can add an amount of trace element simultaneously.
Pass through to add H in the above-mentioned trace element
3BO
3, MnSO
47H
2O, ZnSO
47H
2O, Cu SO
45H
2O, (NH
4)
6Mo
7O
244H
2O, Co (NO
3) 6H
2O provides.
The application of microbial inoculum for degrading polycyclic aromatic hydrocarbons of the present invention in the degraded organic aromatic compound, wherein said organic aromatic compound is a polycyclic aromatic hydrocarbons, it comprises pyrene, phenanthrene, fluoranthene, fluorenes, naphthalene, anthracene, acenaphthene.
Near the mud of the pale yellow mycobacterium W52 strains separation of the present invention Ox Mountain coke-oven plant is crouched in the Xuzhou, near pale yellow mycobacterium M16 strains separation of the present invention mud coke-oven plant, Shaoyang, Hunan.
The morphological feature of pale yellow mycobacterium W52 of the present invention is: W52 naked eyes behind 30 ℃ of constant temperature culture 5d just can be seen single clearly bacterium colony.Originally bacterium colony is oyster white on nutrition gravy flat board, and along with the increase of cell age, color is more and more darker, and last W52 bacterial strain is safran.It is round that bacterium colony is rule, middle slightly projection, smooth surface, neat in edge, quality thickness.Examine under a microscope, thalline all is shaft-like slightly crooked, does not produce spore, does not move no gemma, no pod membrane.Gramstaining is weak positive, and acid-fast stain is positive.
The physiological and biochemical property of pale yellow mycobacterium W52 of the present invention is: bacterial strain W52 can grow with 5%NaCl, can reduce nitrate, tellurite, tween hydrolysis (10d), catalase are positive, the urase positive, unfermentable glucose produces acid, can produce acid from pectinose, trehalose, fructose, semi-lactosi, rhamnosyl, can not grow for 45 ℃, abortion within the first month of pregnancy pigment, W52 can be the sole carbon source growth with oxysuccinic acid and succsinic acid, determines tentatively that in view of the above W52 is the quick growth group's of Mycobacterium a scotochromogen.
The morphological feature of pale yellow mycobacterium M16 of the present invention is: M16 naked eyes behind 30 ℃ of constant temperature culture 5d just can be seen single clearly bacterium colony.Originally bacterium colony is oyster white on nutrition gravy flat board, and along with the increase of cell age, color is more and more darker, and it is yellow that M16 finally is.It is round that bacterium colony all is rule, smooth surface, neat in edge.Behind the gramstaining, easy coloring not, acid-fast stain is all positive.Cell is shaft-like, does not produce spore, does not move no gemma.
The physiological and biochemical property of pale yellow mycobacterium M16 of the present invention is: M16 also can grow with 5%NaCl, can reduce nitrate, and tween hydrolysis (5d), catalase are positive, but the urase experiment is positive.Unfermentable glucose produces acid, can not produce acid from mannitol, but can produce acid from pectinose, rhamnosyl, sorbyl alcohol, melampyrin, trehalose, inositol, fructose, semi-lactosi.M16 can only be the sole carbon source growth with the oxysuccinic acid.Determine tentatively that in view of the above M16 is the quick growth group's of Mycobacterium a scotochromogen.
The cultural method of pale yellow mycobacterium W52 bacterial strain of the present invention:
Bacterial strain is joined in the nutrition bouillon media 30 ℃, 170rmin
-1Shaking culture 3d.
The nutrition bouillon media, composition and consumption (g/L): extractum carnis 3, peptone 10, NaCl5, agar 20 is adjusted pH7.0-7.2.
The cultural method of pale yellow mycobacterium M16 bacterial strain of the present invention:
Bacterial strain is joined the nutrition bouillon media, 30 ℃, 170rmin
-1Shaking culture 3d.
The nutrition bouillon media, composition and consumption (g/L): extractum carnis 3, peptone 10, NaCl5, agar 20 is adjusted pH7.0-7.2.
Form by 2 bacterial classifications such as pale yellow mycobacterium W52, pale yellow mycobacterium M16 of the safety of multiple three ring PAHs of degraded and Fourth Ring PAHs by microbiobacterial agent of the present invention.Have the ability of multiple polycyclic aromatic hydrocarbons in the dissolving soil, be fit to south rice soil, vegetable garden soil, red soil, and the soil types such as moisture soil in the north, the unusual effect of repairing polycyclic aromatic hydrocarbon pollution had.Simultaneously, can promote plant growth, raising crop yield.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
The preparation of embodiment 1 microbial inoculum for degrading polycyclic aromatic hydrocarbons
The fermentor cultivation liquid formula is: beef broth 0.5%, and peptone 0.5%, NaCl 0.5%, soya-bean oil 0.5%, glucose 0.5%, sal epsom 0.08%, potassium primary phosphate 0.04%, Cu, Zn, Mn, the Fe of lime carbonate 0.5%, 800 μ M stir.PH value 7.6 before the sterilization, sterilization back pH value is 7.4.
W52 and M16 bacterial strain are distinguished streak inoculation on the beef broth protein culture medium, cultivate 48h for 28-30 ℃, insert the 500mL triangular flask then respectively,, under the 180r/min, cultivate 18h at 30 ℃.Be linked in the 15L seeding tank by 1% inoculum size then, at 180r/min, pH7.5, under the air flow 0.5vvm, cultivate 24h after, be encased in the fermentor tank of 100L by 5% inoculum size again, at 200r/min, pH7.5 under the air flow 0.6vvm, cultivates 3d.
After fermentation is finished, W52, M16 and the peat composed of rotten mosses are added the peat composed of rotten mosses or the diatomite of the good bacterium of death of monks or nuns according to 1: 1: 12 ratio with bacterial strain respectively, add 0.1% trace element (H simultaneously
3BO
357mg/L, MnSO
47H
2O 43mg/L, ZnSO
47H
2O 43mg/L, CuSO
45H
2O 40mg/L, (NH
4)
6Mo
7O
244H
2O 37mg/L, Co (NO
3) 6H
2O25mg/L), aseptic pack is sealed, preservation under room temperature.
The experiment of experimental example 1 bacteria agent degrading polycyclic aromatic hydrocarbons of the present invention
The inorganic salt basic medium, composition and consumption (g/L): NH
4Cl 1.1, K
2HPO
41.0 NaCl 0.5, KCl 0.2, MgSO
47H
2O 0.2, FeSO
40.001, CaCl
20.01, add micro-mixing solutions 1ml (H again
3BO
357mg/L, MnSO
47H
2O43mg/L, ZnSO
47H
2O43mg/L, CuSO
45H
2O40mg/L, (NH
4)
6Mo
7O
244H
2O37mg/L, Co (NO
3) 6H
2O 25mg/L), add 2% agar in the solid medium.
Containing on the solid plate of polycyclic aromatic hydrocarbons, the bacterium liquid of inoculating the embodiment 1 of 10 μ L (contains bacterium 10
8Cfu/mL), 30 ℃ of cultivations.Degradation bacteria W52 and M16 the 3rd, 7,14 and the degraded circle of 21d and colony diameter see Table 1 and table 2, according to the PAHs content of dull and stereotyped unit surface, obtain degradation bacteria at last the degradation amount of solid PAHs seen Table 3.
Degraded circle and the colony diameter (mm) of table 1 degradation bacteria W52 on the nutrient agar that contains phenanthrene, fluoranthene and pyrene epitaxial
Degraded circle and the colony diameter (mm) of table 2 degradation bacteria M16 on the nutrient agar that contains phenanthrene, fluoranthene and pyrene epitaxial
The degradation amount (* 10 of table 3 degradation bacteria strains W52 and M16
-2Mg)
According to the size of observing degraded circle and bacterium colony every day, as can be seen, containing on the flat board of luxuriant and rich with fragrance epitaxial, the degraded circle of two strain bacterium is bigger, and bacterium colony is also bigger, relatively, the growth of degraded circle is slower on the flat board of fluoranthene and pyrene, degraded circle and bacterium colony are all less, and this is because phenanthrene is low-molecular-weight polycyclic aromatic hydrocarbons, more easily degraded; On the high flat board of PAHs content, the degraded circle is less, and growing way is slower.
Bacterial strain W52 reaches and bacterial strain M16 containing on the agar solid plate of pyrene degraded pyrene ability, and degraded ability luxuriant and rich with fragrance and fluoranthene then is lower than bacterial strain M16.Bacterial strain W52 degraded pyrene 7.2 * 10 when cultivating 3d
-2Mg is than the degradation amount (3.9 * 10 of bacterial strain M16
-2Mg) improve 84.5%; Bacterial strain W52 degraded pyrene 36.6 * 10 when cultivating 21d
-2Mg is than the degradation amount (20.1 * 10 of bacterial strain M16
-2Mg) improve 82.1%.Show that bacterial strain W52 is more suitable for being used for the degraded of pyrene.
Experimental example 2 bacteria agents of the present invention are to the reparation experiment of pyrene contaminated soil
In sterilization and unsterilised soil, add pyrene 200mg/kg soil, add urea 125mg/kg soil, Repone K 125mg/kg soil, potassium primary phosphate 125mg/kg soil, plantation rape and Plantula Brassicae chinensis, microbial inoculum (combination of W52 and M16) the 4g/kg soil of adding embodiment 1, W52, M16 bacterial strain use respectively as the second stage and contrast, usage quantity is similarly 4g/kg soil, plants in the greenhouse.After 2 weeks of plant-growth, 4 weeks, 6 weeks, the residual quantity of pyrene in the sampling and measuring soil is used SAS software and is carried out data processing, and the result is shown in table 4, table 5 and table 6.
Pyrene residual quantity (mg/kg) in the soil of different treatment after 2 weeks of table 4 inoculation
Annotate: on behalf of the difference between different treatment, different letters reach 0.05 level.
Pyrene residual quantity (mg/kg) after 4 weeks of table 5 inoculation in the different treatment soil
Annotate: on behalf of the difference between different treatment, different letters reach 0.05 level.
Pyrene residual quantity (mg/kg) after 6 weeks of table 6 inoculation in the different treatment soil
Table 4 is the result show: under the soil disinfection condition, the plantation Plantula Brassicae chinensis is after 2 weeks, inoculate microbial inoculum and do not inoculate microbial inoculum notable difference is arranged, not inoculating the microbial inoculum degradation rate is 51.7%, drop to 15.55mg/kg by the soil pyrene 200mg/kg before the inoculation behind the inoculation microbial inoculum W52, degradation rate reaches 92.2%, inoculation microbial inoculum M16 drops to 15.86mg/kg by the preceding soil pyrene 200mg/kg of inoculation, degradation rate is 92.1%, and inoculation microbial inoculum W52 and M16 are 94.2% by the degradation rate that the preceding soil pyrene 200mg/kg of inoculation drops to 11.64mg/kg; Behind the plantation rape, the degradation rate of inoculation microbial inoculum soil pyrene is greater than Chinese cabbage.The combination degradation rate effect of inoculation microbial inoculum W52 and M16 is best, and soil pyrene 200mg/kg drops to 7.94mg/kg, and degradation rate reaches 97.6%.
Under the unsterilised condition, the effect of bacterial strain W52 and M16 combination inoculation Plantula Brassicae chinensis degraded soil pyrene is greater than sterilising conditions, and degradation rate is 97.6%, and the pyrene degradation rate of rape inoculation W52 and M16 combination reaches 96.7%.With unsterilised, plant Plantula Brassicae chinensis during all are handled, the combination degradation rate of inoculation microbial inoculum W52 and M16 is the highest.Plantation rape, the soil pyrene concentration of not using microbial inoculum to contrast are lower than plantation Chinese cabbage, illustrate that the ability of rape self degraded pyrene is stronger.
Table 5 result shows that the pyrene residual quantity after 4 weeks of inoculation in the different treatment soil descends when all than 2.Under soil disinfection plantation Chinese cabbage and the unsterilised plantation rape of the soil condition, inoculation microbial inoculum and the significant difference of not inoculating microbial inoculum.There were significant differences between three kinds of microbial inoculums were handled.
Soil disinfection, plantation Chinese cabbage inoculation W52 and M16 microbial inoculum pyrene degradation rate 98.77%, plantation rape pyrene degradation rate 98.47%; The degradation rate 96.67% of the unsterilised plantation of soil Chinese cabbage inoculation W52 and M16 microbial inoculum pyrene, the degradation rate 98.47% of the pyrene of plantation rape.The soil pyrene degradation rate of all inoculation W52 and M16 microbial inoculum all is significantly higher than W52, M16 single inoculation.
Table 6 result shows that when the 6th week, the pyrene degradation rate of all inoculation W52 and M16 combination microbial inoculum all is significantly higher than W52, M16 single inoculation.
The pyrene degradation rate 99.83% of soil disinfection, plantation Chinese cabbage inoculation W52 and M16 microbial inoculum, plantation rape pyrene degradation rate 99.85%; The degradation rate 99.82% of the unsterilised plantation of soil Chinese cabbage inoculation W52 and M16 microbial inoculum pyrene, the degradation rate 99.86% of the pyrene of plantation rape.(bacterial strain W52 and M16 microbial inoculum can effectively be degraded equally to pyrene 2～4mg/kg) time at low levels.
Experimental example 3 bacteria agents of the present invention are to the influence of phytomass
Biomass data after the plant results of experimental example 2 are as shown in table 7.
The phytomass of table 7 different treatment (g/ basin)
In sterile soil, inoculation W52, M16, W52 and M16 combination (bacteria agent of embodiment 1) three connect the Chinese cabbage of bacterium, the biomass of rape is higher than blank, and the biomass of W52 and M16 combination inoculation is higher than single inoculation.
In the unsterilised soil, inoculation W52, M16, W52 and the rape of M16 combination microbial inoculum and the biomass of Chinese cabbage all are higher than blank.The Chinese cabbage of W52 and M16 combination inoculation, the biomass of rape are higher than W52, M16 single inoculation.The plant biomass all is higher than in the sterile soil in the unsterilised soil.Show that pyrene degradation bacteria W52 and M16 have the effect that promotes growth to plant in contaminated soil.
Experimental example 4 plant roots and cauline leaf are to the absorption of pyrene
The plant root of the different treatment that experimental example 3 obtains and the pyrene content of cauline leaf, as shown in table 8.
The pyrene content (ng/g) of plant root and cauline leaf under the table 8 different treatment condition
The result shows, still is the cauline leaf part at root no matter, and the pyrene amount of the crop accumulation of inoculation polycyclic aromatic hydrocarbon degrading bacteria all is lower than the contrast of not inoculating microbial inoculum.
Under the unsterilised condition, the cauline leaf absorption pyrene amount that Chinese cabbage is inserted microbial inoculum is higher than sterile soil Chinese cabbage suction pyrene amount, and the rape that connects bacterium is inhaled the suction pyrene amount that the pyrene amount is higher than unsterilised rape.Inoculation W52 and M16 mix bacterium agent, the suction pyrene amount of Chinese cabbage, rape significantly is lower than W52, M16 uses separately.Use polycyclic aromatic hydrocarbon degrading bacteria can reduce crop to pyrene absorbed dose.
Claims (7)
1. microbial inoculum for degrading polycyclic aromatic hydrocarbons, its effective constituent is:
Pale yellow mycobacterium (Mycobacterium gilvum) W52, preserving number CGMCC NO.2274;
Pale yellow mycobacterium (Mycobacterium gilvum) M16, preserving number CGMCC NO.2273.
2. microbial inoculum as claimed in claim 1, it comprises the component of following weight part:
Pale yellow mycobacterium W52 1-5 part;
Pale yellow mycobacterium M16 1-5 part;
Additive 10-15 part.
3. microbial inoculum as claimed in claim 2, its feature comprises the component of following weight part:
1 part of pale yellow mycobacterium W52;
1 part of pale yellow mycobacterium M16;
12 parts of additives.
4. as claim 2 or 3 described microbial inoculums, it is characterized in that described additive is the peat composed of rotten mosses or diatomite.
5. the application of the arbitrary described microbial inoculum of claim 1-4 in the degraded organic aromatic compound.
6. application as claimed in claim 5 is characterized in that, described organic aromatic compound is a polycyclic aromatic hydrocarbons.
7. application as claimed in claim 6 is characterized in that, described polycyclic aromatic hydrocarbons is pyrene, phenanthrene, fluoranthene, fluorenes, naphthalene, anthracene or acenaphthene.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101974442B (en) * | 2010-03-02 | 2011-12-07 | 北京师范大学 | Pyrene degrading bacterium and application thereof |
| CN102533578B (en) * | 2010-12-10 | 2013-12-25 | 中国农业大学 | Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon |
| CN102533575B (en) * | 2010-12-13 | 2013-10-02 | 北京师范大学 | Strain capable of degradation of benzo[alpha]pyrene |
| CN102277350B (en) * | 2011-08-06 | 2013-04-10 | 浙江大学 | Single-carrier polycyclic aromatic hydrocarbon degrading bacterium and preparation method thereof |
| CN102392012B (en) * | 2011-08-08 | 2013-05-01 | 浙江大学 | Polycyclic aromatic hydrocarbon antibacterial agent of composite carrier and preparation method of polycyclic aromatic hydrocarbon antibacterial agent |
| CN103555612B (en) * | 2013-10-14 | 2015-06-03 | 华南理工大学 | Light yellow mycobacterium and its application in degradation of oil components polycyclic aromatic hydrocarbons |
| CN105921510A (en) * | 2016-05-06 | 2016-09-07 | 南京师范大学 | Restoration method special for oryza sativa L. polluted by polycyclic aromatic hydrocarbon phenanthrene |
| CN107597825A (en) * | 2017-09-30 | 2018-01-19 | 青岛农业大学 | The method and system of in-situ chemical and Co-bioremediation polycyclic aromatic hydrocarbon pollution |
| CN109370931B (en) * | 2018-01-17 | 2022-08-12 | 轻工业环境保护研究所 | Complex microbial inoculant for efficiently degrading polycyclic aromatic hydrocarbon and application thereof |
| CN113462609B (en) * | 2021-07-30 | 2022-08-19 | 中国科学院南海海洋研究所 | Mycobacterium for efficiently degrading polycyclic aromatic hydrocarbons and application thereof |
| CN115415299A (en) * | 2022-08-29 | 2022-12-02 | 东华大学 | Method for repairing pyrene contaminated soil through low-temperature heat treatment-pyrene degradation microbial inoculum combination |
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| CN1644678A (en) * | 2004-07-21 | 2005-07-27 | 山东大学 | Bacteria for degradating thiosulfur efficiently |
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| Willumsen PA et al."Degradation of phenanthrene-analogue azaarenes by Mycobacterium gilvum strain LB307T under aerobic conditions".《Appl Microbiol Biotechnol》.2001,第56卷(第3-4期),539-544页. |
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Assignee: Xuzhou Batian Ecology Co., Ltd. Assignor: Institute of agricultural resources and regional planning, Chinese Academy of Agricultural Sciences Contract record no.: 2012320000364 Denomination of invention: Microbial inoculum for degrading polycyclic aromatic hydrocarbons Granted publication date: 20111012 License type: Exclusive License Open date: 20100210 Record date: 20120330 |
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