CN106883067A - The preparation method of the special anti-blight biological organic fertilizer of strawberry - Google Patents
The preparation method of the special anti-blight biological organic fertilizer of strawberry Download PDFInfo
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- CN106883067A CN106883067A CN201710091539.6A CN201710091539A CN106883067A CN 106883067 A CN106883067 A CN 106883067A CN 201710091539 A CN201710091539 A CN 201710091539A CN 106883067 A CN106883067 A CN 106883067A
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Abstract
The invention provides a kind of preparation method of the special anti-blight biological organic fertilizer of strawberry, with pig manure and maize straw as windrow, No. 1 biocontrol agent of compound microorganism ferments microbial inoculum and bacillus licheniformis ABTNL that inoculation is made up of bacillus subtilis ABTNL 2, bacillus thuringiensis ABTNL 4, bacillus megaterium and colloid bacillus cereus, fermentation, is obtained the biological organic fertilizer with anti-strawberry droop function.Acted synergistically between strain of the present invention, greatly prolong high-temperature duration, shorten the compost maturity cycle；Biological control effectively can be carried out to strawberry droop；The present invention utilizes pig manure and maize straw, and combine compound microorganism ferments microbial inoculum and biocontrol agent, the special anti-blight biological organic fertilizer of strawberry is successfully prepared using a step insertion method, realize the harmless treatment of pig manure and maize straw and recycling, reduce the use of agricultural chemicals and chemical fertilizer, greatly reduce the production cost of biological organic fertilizer.
Description
Technical field
The present invention relates to agricultural biological technical field, the system of more particularly to a kind of special anti-blight biological organic fertilizer of strawberry
Preparation Method.
Background technology
The large-scale use of chemical fertilizer serves important function to the rapid growth of Past 30 Years China agricultural production.Chemical fertilizer is big
Amount also generates serious negative effect using while agricultural production is improved to environment, and threaten China's agricultural can
Sustainable development.The unreasonable administration of chemical fertilizer causes crops quality reduction, causes soil hardening, organic matter decline, soil
And crop root Tiny ecosystem is destructurized, and soil biography crop disease is caused to be spread unchecked.In order to ensure agricultural product quality and safety and agriculture
Industry sustainable development, research novel organic fertilizer material come replace or reduce chemical fertilizer administration it is extremely urgent.Biological organic fertilizer nutrient
Comprehensively, existing various inorganic elements, there is various organic nutrients again, also containing substantial amounts of microorganism and enzyme, can improve and make produce
Amount, the generation for improving crop quality and soil micro-ecosystem system, reducing and reduce crop disease and insect.
Strawberry droop is one of the Major Diseases for having a strong impact on Strawberry industry economic benefit and sustainable development, is made in the past
Control strawberry droop with chemical bactericide, but chemical bactericide large area using easily bringing environmental pollution, disruption of ecological balance, medicine
The problems such as thing residual, food security, so using low cost, high efficiency, environment-friendly and drug residue free biological method
Prevention and control strawberry droop receives much attention.
The content of the invention
It is an object of the invention to by setting up aerobic static composting system, realize harmless treatment and the money of agricultural wastes
Sourceization is utilized, and prepares a kind of special anti-blight biological organic fertilizer of strawberry, optimizes the production technology of biological organic fertilizer, reduces production
Cost.
In order to achieve the above object, the invention provides a kind of preparation side of the special anti-blight biological organic fertilizer of strawberry
Method, with pig manure and maize straw as windrow, is inoculated with by bacillus subtilis No. ABTNL-2, bacillus thuringiensis ABTNL-4
Number, bacillus megaterium and colloid bacillus cereus composition compound microorganism ferments microbial inoculum and bacillus licheniformis No. ABTNL-1
Biocontrol agent, fermentation is obtained the biological organic fertilizer with anti-strawberry droop function.
The bacillus subtilis ABTNL-2 deposit numbers：CGMCC NO.10038, bacillus thuringiensis ABTNL-
No. 4 deposit numbers：CGMCC NO.10036, bacillus licheniformis ABTNL-1 deposit numbers：CGMCC NO.10037；It is huge
Bacillus and colloid bacillus cereus can be obtained from China General Microbiological culture presevation administrative center, it is also possible to by voluntarily dividing
From obtaining, in addition to special bacterial strain situation, this universal 2 kinds of microbial inoculums are suitable for the present invention.
Under preferred embodiment, the preparation method of the above-mentioned special anti-blight biological organic fertilizer of strawberry is concretely comprised the following steps：
The pretreatment of S1, compost raw material：Pig manure and particle diameter≤1cm maize straws are pressed 3~4.5:1~1.5 quality
Than being well mixed, it is 20~25 to control C/N:1, dry 2~3 days, to moisture content up to 50~60%, windrow is obtained, it is standby；
The preparation of S2, compound microorganism ferments microbial inoculum and biocontrol agent：
By bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, bacillus megaterium, colloid gemma
Bacillus, bacillus licheniformis No. ABTNL-1 individually carry out fermenting and producing, and 10 are reached to bacterial concentration8~109CFU·ml-1；
By bacillus subtilis ABTNL-2 bacterium solutions after fermentation, bacillus thuringiensis ABTNL-4 bacterium solutions, huge
Bacillus bacterium solution and colloid bacillus cereus bacterium solution press percent by volume 1~2:1~2：0.5~1:0.5~1 mixing, is answered
Close microbial fermentation inoculum；
The bacillus licheniformis ABTNL-1 bacterium solutions are biocontrol agent；
S3, the foundation of aerobic static composting system and the preparation of biological organic fertilizer：
By compound microorganism ferments microbial inoculum and biocontrol agent obtained in step S2, while being inoculated into windrow obtained in step S1
In, aerobic static composting system is set up, fermentation 20~30 days of becoming thoroughly decomposed obtains the special anti-blight biological organic fertilizer of strawberry；
The compound microorganism ferments microbial inoculum volume is 2.0~5.0 with the windrow mass ratio:100(g/ml)；
The biocontrol agent volume is 2.0~5.0 with the windrow mass ratio:100(g/ml).
Further optimization, the culture medium of fermentation process is respectively described in step S2：
It is bacillus subtilis No. ABTNL-2 in compound microorganism ferments microbial inoculum, bacillus thuringiensis No. ABTNL-4, huge
The medium component of Bacterium anthracoides and colloid bacillus cereus is peptone 10.0g/L, extracted beef powder 3.0g/L, sodium chloride
5.0g/L, pH to 7.4 is adjusted with 5mol/L NaOH；
The medium component that biocontrol agent bacillus licheniformis is No. ABTNL-1 is sodium chloride 10.0g/L, tryptone
10.0g/L, yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
Further optimization, the become thoroughly decomposed detailed processes of fermentation of step S3 are：Enter when windrow temperature occurs being decreased obviously first
Row turning, and it is 35~45% to keep the skin wet to moisture content, windrow temperature is raised again, and windrow temperature is decreased obviously again
When, turning again, so repeatedly 2~3 times, until windrow temperature close to or a little higher than room temperature, free from extraneous odour, and windrow hair after measured
When bud index is 30% more than or equal to 80%, moisture content, worm's ovum content is 0%, that is, obtain composite fermentation product.
When windrow germination index is more than 80%, show that compost has become thoroughly decomposed, windrow moisture content is about 30%；Windrow is used as biology
Organic fertilizer early stage product, saves backup；The present invention is become thoroughly decomposed and is fermented 20-30 days.
Think to be decreased obviously windrow temperature drop >=5 DEG C of the present invention；After turning, when windrow temperature is no longer raised, just
Without turning again.
The inventive method mainly includes two steps：
One is the application of compound microorganism ferments microbial inoculum and biocontrol agent, and substantially increasing fermentation efficiency, shortening biology has
Machine fertilizer manufacturing cycle；The anti-strawberry droop effect of fertilizer is improve simultaneously.
Two is the foundation of aerobic static composting system and the preparation of biological organic fertilizer, and the complex microorganism of above-mentioned preparation is sent out
Yeast-like fungi agent and biocontrol agent are set up the initial stage and are accessed simultaneously in composting system, complete to ferment by the production technology of a step insertion
Journey, so as to the biological organic fertilizer with anti-strawberry droop is obtained.
To sum up, compared with prior art, the advantage of the invention is that：
1st, by the use of fresh pig manure and maize straw as the raw material of production biological organic fertilizer, can synchronously realize that agricultural is useless
The harmless treatment of gurry and recycling, it is to avoid it deals with the secondary pollution caused to environment improperly.
2nd, the present invention is inoculated with homemade compound microorganism ferments microbial inoculum and biocontrol agent simultaneously at the compost initial stage, wherein, it is multiple
Closing microbial fermentation inoculum can accelerate the maturity of aerobic static composting system, shorten the manufacturing cycle of biological organic fertilizer；
And bacillus licheniformis is a kind of bacterium with broad-spectrum antibacterial activity, and ability is produced with stronger secondary metabolite,
Various antibacterial substances can be produced, the present invention adds the secondary metabolite produced by biocontrol microorganisms, can effectively to strawberry droop
Carry out biological control, it is to avoid the environmental problem and food-safety problem brought using chemical bactericide.
3rd, the present invention prepares biological organic fertilizer by a step insertion composting fermentation technology, i.e., the heap initial stage is built, inoculation is multiple simultaneously
Microbial fermentation inoculum and biocontrol agent are closed, compared to secondary fermentation, production technology is simpler, and will substantially reduce biology has
The production cost of machine fertilizer.
4th, the obtained biological organic fertilizer of the present invention has improved soil, promotes fertilizer efficiency, improves rhizosphere of strawberry Tiny ecosystem and grass
The advantages of certain kind of berries quality, enhancing strawberry resistance, the sustainable development to strawberry cultivating industry provides sound assurance.
Brief description of the drawings
Fig. 1 is aerobic static composting system temperature variation diagram；
Fig. 2 is biological and ecological methods to prevent plant disease, pests, and erosion experimental result of the biological organic fertilizer of comparative example 3 to strawberry droop
Fig. 3 is biological and ecological methods to prevent plant disease, pests, and erosion experimental result of the biological organic fertilizer of the embodiment of the present invention 2 to strawberry droop
Preservation explanation
The preservation information of biological material specimens of the present invention：The microorganism (strain) for joining evidence is ABTNL-1, Classification And Nomenclature
It is bacillus licheniformis (Bacillus licheniformis), on November 21st, 2014 by Chinese microorganism strain preservation pipe
The reason committee common micro-organisms center (abbreviation CGMCC) preservation, deposit number CGMCC NO.10037.CGMCC addresses are Beijing
The institute 3 of city Chaoyang District North Star West Road 1.
The preservation information of biological material specimens of the present invention：The microorganism (strain) for joining evidence is ABTNL-2, Classification And Nomenclature
It is bacillus subtilis (Bacillus subtilis) to be managed by Chinese microorganism strain preservation on November 21st, 2014 and entrusted
Member can common micro-organisms center (abbreviation CGMCC) preservation, deposit number CGMCC NO.10038.CGMCC addresses are court of Beijing
The institute 3 of positive area's North Star West Road 1.
The preservation information of biological material specimens of the present invention：The microorganism (strain) for joining evidence is ABTNL-4, Classification And Nomenclature
It is bacillus thuringiensis (Bacillus thuringiensis), on November 21st, 2014 by Chinese microorganism strain preservation
Administration committee common micro-organisms center (abbreviation CGMCC) preservation, deposit number CGMCC NO.10036.CGMCC addresses are north
The institute 3 of Jing Shi Chaoyang Districts North Star West Road 1.
Specific embodiment
With reference to specific embodiment and comparative example, the specific effect that the present invention is furture elucidated.
Embodiment 1
A kind of special anti-blight biological organic fertilizer of strawberry, is obtained by the production method for comprising the following steps：
(1) pretreatment of compost raw material
Fresh pig manure and particle diameter≤1cm maize straws are pressed 3:1 mass ratio is well mixed, and makes its C/N be 20, divides
Ground dries to moisture content and (holds a windrow with hand-tight, do not have liquid to flow out between finger, and windrow after loosing one's grip up to 50% standby
In bulk not loosely).
(2) preparation of compound microorganism ferments microbial inoculum and biocontrol agent
By bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, bacillus megaterium, colloid gemma
Bacillus and bacillus licheniformis No. ABTNL-1 individually carry out fermenting and producing using fermentation tank, bacterial concentration is reached 108~
109CFU·ml-1, the bacillus subtilis No. ABTNL-2 after fermentation, bacillus thuringiensis No. ABTNL-4, huge gemma bar
Bacterium and colloid bacillus cereus bacterium solution press percent by volume 2：2：1:1 mixing is used as compound microorganism ferments microbial inoculum, lichens gemma bar
Bacterium ABTNL-1 zymocyte liquids are used as biocontrol agent.
Medium component needed for the preparation of compound microorganism ferments microbial inoculum is peptone 10.0g/L, extracted beef powder 3.0g/
L, sodium chloride 5.0g/L, pH to 7.4 is adjusted with 5mol/L NaOH；Medium component needed for the preparation of biocontrol agent is sodium chloride
10.0g/L, tryptone 10.0g/L, yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
(3) foundation of aerobic static composting system and the preparation of biological organic fertilizer
The composting material that (1) pre-processes manually fully is mixed, is fitted into (100cm in the compost reaction chamber for be placed with breather pipe
× 80cm × 80cm) aerobic static composting system is set up, while the liquid microbial inoculum prepared by inoculation (2), compound microorganism ferments
Microbial inoculum and biocontrol agent are inoculated into aerobic static composting system by 2% and 5% (volume mass ratio) respectively, and specific method is such as
Under：It is layered in compost reactor and places material, and uniformly after sprinkling liquid microbial inoculum is sufficiently mixed and carries out microorganism aerobic fermentation,
Temperature rises therewith, reaches and maintain 60~75 DEG C of high temperature；Turning is carried out when windrow temperature occurs being decreased obviously first, and
The certain moisture of supplement, windrow temperature is raised again, equally, when temperature is decreased obviously, turning again, so repeatedly 2-3 times,
When windrow temperature is approached or a little higher than room temperature and free from extraneous odour, determine germination index and be about 30%, worm's ovum more than 80%, moisture content
When content is 0%, show that biological organic fertilizer early stage product has been successfully prepared, windrow is crushed, is sieved, be made powder, can
Needed to granulate etc. according to business, a kind of special anti-blight biological organic fertilizer of strawberry is obtained.
After testing, the component target of the special anti-withered biological organic fertilizer of finished product strawberry that the present embodiment is obtained is as shown in table 1：
Table 1
Embodiment 2
Step 1：The pretreatment of compost raw material
Fresh pig manure and particle diameter≤1cm maize straws are pressed 4.5:1.5 mass ratio is well mixed, and makes its C/N 25, puts down
Be spread out in ground dry it is standby up to 60% to moisture content.
Step 2：The preparation of compound microorganism ferments microbial inoculum and biocontrol agent
By bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, bacillus megaterium, colloid gemma
Bacillus and bacillus licheniformis No. ABTNL-1 individually carry out fermenting and producing using fermentation tank, bacterial concentration is reached 108~
109CFUml-1, the bacillus subtilis No. ABTNL-2 after fermentation, bacillus thuringiensis No. ABTNL-4, huge gemma bar
Bacterium and colloid bacillus cereus bacterium solution press percent by volume 1：1：0.5:0.5 mixing is used as compound microorganism ferments microbial inoculum, lichens bud
Spore bacillus ABTNL-1 zymocyte liquids are used as biocontrol agent.
Medium component needed for the preparation of compound microorganism ferments microbial inoculum is peptone 10.0g/L, extracted beef powder 3.0g/
L, sodium chloride 5.0g/L, pH to 7.4 is adjusted with 5mol/L NaOH；Medium component needed for the preparation of biocontrol agent is sodium chloride
10.0g/L, tryptone 10.0g/L, yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
Step 3：The foundation of aerobic static composting system and the preparation of biological organic fertilizer
Pretreated compost raw material are added in the solid state fermentation storehouse with agitating function, are made by mechanical agitation
It is fully mixed, respectively toward 5% (volume mass ratio) composite microbial of inoculation in solid state fermentation storehouse while mechanical agitation is mixed
Thing fermenting agent and 4% (volume mass ratio) biocontrol agent, carry out semiclosed aerobic fermentation.
Detection compost temperature, testing result is as shown in figure 1, compost 3d temperature is to reach more than 55 DEG C, and is reached in 5d
To 61.34 DEG C of highest, temperature continues 11d for more than 55 DEG C, has reached China《Decontamination of human excreta sanitary standard (GB7959-87)》
Standard (more than 55 DEG C continue more than 3d), 23d or so compost becomes thoroughly decomposed completely.Determine germination index more than 80%, moisture content about
For 30% when, worm's ovum content be 0% when, show that biological organic fertilizer early stage product has been successfully prepared, windrow is crushed, mistake
Sieve, is made powder, can be needed to granulate etc. according to business, and a kind of special anti-blight biological organic fertilizer of strawberry is obtained.
The component target of the special anti-blight biological organic fertilizer of finished product strawberry that production is obtained is as shown in table 2：
Table 2
Comparative example 1 (embodiment 2 lacks bacillus subtilis ABTNL-2)：
Step 1：The pretreatment of compost raw material
Fresh pig manure and particle diameter≤1cm maize straws are pressed 4.5:1.5 mass ratio is well mixed, make its C/N 25 it
Between, divide dried on ground it is standby up to 60% to moisture content.
Step 2：The preparation of compound microorganism ferments microbial inoculum and biocontrol agent
By bacillus thuringiensis No. ABTNL-4, bacillus megaterium, colloid bacillus cereus and bacillus licheniformis
No. ABTNL-1 individually carries out fermenting and producing using fermentation tank, bacterial concentration is reached 108~109CFUml-1, after fermentation
Bacillus thuringiensis No. ABTNL-4, bacillus megaterium and colloid bacillus cereus bacterium solution press percent by volume 1：0.5:0.5
Used as compound microorganism ferments microbial inoculum, bacillus licheniformis ABTNL-1 zymocyte liquids are used as biocontrol agent for mixing.
Medium component needed for the preparation of compound microorganism ferments microbial inoculum is peptone 10.0g/L, extracted beef powder 3.0g/
L, sodium chloride 5.0g/L, pH to 7.4 is adjusted with 5mol/L NaOH；Medium component needed for the preparation of biocontrol agent is sodium chloride
10.0g/L, tryptone 10.0g/L, yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
Step 3：The foundation of aerobic static composting system and the preparation of biological organic fertilizer
Pretreated compost raw material are added in the solid state fermentation storehouse with agitating function, are made by mechanical agitation
It is fully mixed, respectively toward 5% (volume mass ratio) composite microbial of inoculation in solid state fermentation storehouse while mechanical agitation is mixed
Thing fermenting agent and 4% (volume mass ratio) biocontrol agent, carry out semiclosed aerobic fermentation.
Compost 5d temperature is to reach more than 55 DEG C, and reaches 60.98 DEG C of highest in 6d, and temperature continues for more than 55 DEG C
7d, has reached China《Decontamination of human excreta sanitary standard (GB7959-87)》Standard (more than 55 DEG C continue more than 3d), 27d is left
Right compost becomes thoroughly decomposed completely.Determine germination index more than 80%, moisture content be about 30%, worm's ovum content for 0% when, show that biology has
Machine fertilizer early stage product has been successfully prepared, and windrow is crushed, is sieved, and is made powder, can be needed to granulate etc. according to business,
A kind of special anti-blight biological organic fertilizer of strawberry is obtained.
Summarize：As seen from the above comparison：The composite bacteria agent for lacking bacillus subtilis ABTNL-2 bacterium makes the height of compost
Warm decreased duration 4d, time lengthening of becoming thoroughly decomposed 4d, illustrate that it plays a significant role in composite bacteria agent.
Comparative example 2 (embodiment 2 lacks bacillus thuringiensis ABTNL-4)：
Step 1：The pretreatment of compost raw material
Fresh pig manure and particle diameter≤1cm maize straws are pressed 4.5:1.5 mass ratio is well mixed, and makes its C/N 25, puts down
Be spread out in ground dry it is standby up to 60% to moisture content.
Step 2：The preparation of compound microorganism ferments microbial inoculum and biocontrol agent
By bacillus subtilis No. ABTNL-2, bacillus megaterium, colloid bacillus cereus and bacillus licheniformis ABTNL-
No. 1 individually carries out fermenting and producing using fermentation tank, bacterial concentration is reached 108~109CFUml-1, the withered grass after fermentation
Bacillus No. ABTNL-2, bacillus megaterium and colloid bacillus cereus bacterium solution press percent by volume 1：0.5:0.5 mixing conduct
Compound microorganism ferments microbial inoculum, bacillus licheniformis ABTNL-1 zymocyte liquids are used as biocontrol agent.
Medium component needed for the preparation of compound microorganism ferments microbial inoculum is peptone 10.0g/L, extracted beef powder 3.0g/
L, sodium chloride 5.0g/L, pH to 7.4 is adjusted with 5mol/L NaOH；Medium component needed for the preparation of biocontrol agent is sodium chloride
10.0g/L, tryptone 10.0g/L, yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
Step 3：The foundation of aerobic static composting system and the preparation of biological organic fertilizer
Pretreated compost raw material are added in the solid state fermentation storehouse with agitating function, are made by mechanical agitation
It is fully mixed, respectively toward 5% (volume mass ratio) composite microbial of inoculation in solid state fermentation storehouse while mechanical agitation is mixed
Thing fermenting agent and 4% (volume mass ratio) biocontrol agent, carry out semiclosed aerobic fermentation.
Compost 4d temperature is to reach more than 55 DEG C, and reaches 60.48 DEG C of highest in 7d, and temperature continues for more than 55 DEG C
6d, has reached China《Decontamination of human excreta sanitary standard (GB7959-87)》Standard (more than 55 DEG C continue more than 3d), 26d is left
Right compost becomes thoroughly decomposed completely.Determine germination index more than 80%, moisture content be about 30%, worm's ovum content for 0% when, show that biology has
Machine fertilizer early stage product has been successfully prepared, and windrow is crushed, is sieved, and is made powder, can be needed to granulate etc. according to business,
A kind of special anti-blight biological organic fertilizer of strawberry is obtained.
Summarize：As seen from the above comparison：The composite bacteria agent for lacking bacillus thuringiensis No. ABTNL-4 makes the height of compost
Warm decreased duration 5d, time lengthening of becoming thoroughly decomposed 3d, illustrate that it plays a significant role in composite bacteria agent.
Comparative example 3 (other bacillus licheniformis of embodiment 2 are instead of bacillus licheniformis ABTNL-1)：
Step 1：The pretreatment of compost raw material
Fresh pig manure and particle diameter≤1cm maize straws are pressed 4.5:1.5 mass ratio is well mixed, and makes its C/N 25, puts down
Be spread out in ground dry it is standby up to 60% to moisture content.
Step 2：The preparation of compound microorganism ferments microbial inoculum and biocontrol agent
By bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, bacillus megaterium, colloid gemma
Bacillus and common bacillus licheniformis individually carry out fermenting and producing using fermentation tank, bacterial concentration is reached 108~
109CFUml-1, the bacillus subtilis No. ABTNL-2 after fermentation, bacillus thuringiensis No. ABTNL-4, huge gemma bar
Bacterium and colloid bacillus cereus bacterium solution press percent by volume 1：1：0.5:0.5 mixing as compound microorganism ferments microbial inoculum, commonly
Clothing fermentation of bacillus bacterium solution is used as biocontrol agent.
Medium component needed for the preparation of compound microorganism ferments microbial inoculum is peptone 10.0g/L, extracted beef powder 3.0g/
L, sodium chloride 5.0g/L, pH to 7.4 is adjusted with 5mol/L NaOH；Medium component needed for the preparation of biocontrol agent is sodium chloride
10.0g/L, tryptone 10.0g/L, yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
Step 3：The foundation of aerobic static composting system and the preparation of biological organic fertilizer
Pretreated compost raw material are added in the solid state fermentation storehouse with agitating function, are made by mechanical agitation
It is fully mixed, respectively toward 5% (volume mass ratio) composite microbial of inoculation in solid state fermentation storehouse while mechanical agitation is mixed
Thing fermenting agent and 4% (volume mass ratio) biocontrol agent, carry out semiclosed aerobic fermentation.
Compost 3d temperature is to reach more than 55 DEG C, and reaches 62.04 DEG C of highest in 5d, and temperature continues for more than 55 DEG C
10d, has reached China《Decontamination of human excreta sanitary standard (GB7959-87)》Standard (more than 55 DEG C continue more than 3d), 23d is left
Right compost becomes thoroughly decomposed completely.Determine germination index more than 80%, moisture content when being about 30%, worm's ovum content be when being 0%, shows biology
Organic fertilizer early stage product has been successfully prepared, and windrow is crushed, is sieved, and is made powder, can be needed to be granulated according to business
Deng a kind of prepared special anti-blight biological organic fertilizer of strawberry.
Application examples 1
Biological organic fertilizer obtained in comparative example 3 and the embodiment of the present invention 2 are contained into same amount of bacillus licheniformis
Biological organic fertilizer is uniformly admixed be connected in the sick soil of Strawberry Fusarium Wilt respectively obtained in ABTNL-1, potted plant experiment 2 by a definite date
Month, the observation incidence of disease and biological control effect as shown in Figure 2 and Figure 3, it is found that the strawberry for applying organic fertilizer of the present invention is not presented hair
Disease symptoms, and the strawberry of the biological organic fertilizer containing the common bacillus licheniformis of comparative example 3 is applied, aerial part shows as lobus cardiacus
Change is yellowish green, curling is asymmetric in ship shape, both sides, and blade is matt, and petiole and root vascular bundle become brown or dark brown, subterraneous root
It is dark brown, new root not long is presented the withered disease symptoms of strawberry.
The common bacillus licheniformis of comparative example 3 is obtained by voluntarily extracting, and by repeatedly extraction, carries out test of many times, is imitated
Fruit is similar to, and the bacillus licheniformis ABTNL-1 used with the present invention has significant difference in effect.
Detection hair is carried out by the internal antibiotic synthetic gene of bacillus licheniformis No. ABTNL-1 in biocontrol agent
Existing, the bacterial strain has a potentiality of synthesis Fengycin, Iturin or Bacillomycin, and Fengycin, Iturin and
Bacillomycin is strong fungistat, so bacillus licheniformis No. ABTNL-1 can be effectively withered to strawberry
Disease of withering carries out biological control.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any one skilled in the art in the technical scope of present disclosure, technology according to the present invention scheme and its
Inventive concept is subject to equivalent or change, should all be included within the scope of the present invention.
Claims (4)
1. the preparation method of the special anti-blight biological organic fertilizer of a kind of strawberry, it is characterised in that be with pig manure and maize straw
Windrow, is inoculated with by bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, bacillus megaterium and colloid bud
The compound microorganism ferments microbial inoculum and bacillus licheniformis ABTNL-1 biocontrol agents of spore bacillus composition, fermentation are obtained with anti-
The biological organic fertilizer of strawberry droop function.
2. the preparation method of the special anti-blight biological organic fertilizer of strawberry according to claim 1, it is characterised in that specific step
Suddenly it is：
The pretreatment of S1, compost raw material：Pig manure and particle diameter≤1cm maize straws are pressed 3~4.5:1~1.5 mass ratio is mixed
Close uniform, it is 20~25 to control C/N:1, dry 2~3 days, to moisture content up to 50~60%, windrow is obtained, it is standby；
The preparation of S2, compound microorganism ferments microbial inoculum and biocontrol agent：
By bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, bacillus megaterium, colloid gemma bar
Bacterium, bacillus licheniformis No. ABTNL-1 individually carry out fermenting and producing, and 10 are reached to bacterial concentration8~109CFU·ml-1；
By the bacillus subtilis ABTNL-2 bacterium solutions after fermentation, bacillus thuringiensis ABTNL-4 bacterium solutions, huge gemma
Bacillus bacterium solution and colloid bacillus cereus bacterium solution press percent by volume 1~2:1~2：0.5~1:0.5~1 mixing, obtains compound micro-
Bio-fermentation agents；
The bacillus licheniformis ABTNL-1 bacterium solutions are biocontrol agent；
S3, the foundation of aerobic static composting system and the preparation of biological organic fertilizer：
By compound microorganism ferments microbial inoculum and biocontrol agent obtained in step S2, while be inoculated into windrow obtained in step S1,
Aerobic static composting system is set up, fermentation 20~30 days of becoming thoroughly decomposed obtains the special anti-blight biological organic fertilizer of strawberry；
The compound microorganism ferments microbial inoculum volume is 2.0~5.0 with the windrow mass ratio:100(g/ml)；
The biocontrol agent volume is 2.0~5.0 with the windrow mass ratio:100(g/ml).
3. the preparation method of the special anti-blight biological organic fertilizer of strawberry according to claim 2, it is characterised in that step S2
The culture medium of the fermentation process is respectively：
Bacillus subtilis No. ABTNL-2, bacillus thuringiensis No. ABTNL-4, huge bud in compound microorganism ferments microbial inoculum
The medium component of spore bacillus and colloid bacillus cereus is peptone 10.0g/L, extracted beef powder 3.0g/L, sodium chloride 5.0g/
L, pH to 7.4 is adjusted with 5mol/L NaOH；
The medium component that biocontrol agent bacillus licheniformis is No. ABTNL-1 be sodium chloride 10.0g/L, tryptone 10.0g/L,
Yeast powder extracts 5.0g/L, pH to 7.0 is adjusted with 5mol/L NaOH.
4. the preparation method of the special anti-blight biological organic fertilizer of strawberry according to claim 2, it is characterised in that step S3
The detailed process of fermentation of becoming thoroughly decomposed is：Turning is carried out when windrow temperature occurs being decreased obviously first, and is kept the skin wet to moisture content
It is 35~45%, windrow temperature is raised again, when windrow temperature is decreased obviously again, turning again, so repeatedly 2~3 times,
Until windrow temperature is approached or a little higher than room temperature, free from extraneous odour, and windrow germination index is more than or equal to 80%, moisture content after measured
30%th, when worm's ovum content is 0%, that is, composite fermentation product is obtained.
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| CN108085284A (en) * | 2018-01-24 | 2018-05-29 | 大连理工大学 | Methylotrophic bacillus and the bacterial preparation process comprising the microorganism and application |
| CN110092677A (en) * | 2019-03-08 | 2019-08-06 | 大连理工大学 | A kind of non-forest land plantation ginseng fertilizer special for organic and preparation method thereof |
| CN110195027A (en) * | 2019-03-08 | 2019-09-03 | 大连理工大学 | The preparation method and application of nicotianae ZL-1 and its compost low temperature fermentation inoculum |
| CN110194694A (en) * | 2019-03-08 | 2019-09-03 | 大连理工大学 | The dedicated anti-withered biological organic fertilizer of strawberry and preparation method thereof prepared by ermine fox racoon dog waste |
| CN110668861A (en) * | 2019-11-11 | 2020-01-10 | 中国科学院亚热带农业生态研究所 | Method for composting cow dung by using myriophyllum viridis |
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| CN110195027A (en) * | 2019-03-08 | 2019-09-03 | 大连理工大学 | The preparation method and application of nicotianae ZL-1 and its compost low temperature fermentation inoculum |
| CN110194694A (en) * | 2019-03-08 | 2019-09-03 | 大连理工大学 | The dedicated anti-withered biological organic fertilizer of strawberry and preparation method thereof prepared by ermine fox racoon dog waste |
| CN110195027B (en) * | 2019-03-08 | 2022-03-01 | 大连理工大学 | Tobacco arthrobacter ZL-1 and preparation method and application of compost low-temperature fermentation microbial inoculum thereof |
| CN110668861A (en) * | 2019-11-11 | 2020-01-10 | 中国科学院亚热带农业生态研究所 | Method for composting cow dung by using myriophyllum viridis |
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Application publication date: 20170623 |