CN104263684B - A kind of product siderophore series bacillus and application thereof - Google Patents

A kind of product siderophore series bacillus and application thereof Download PDF

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CN104263684B
CN104263684B CN201410499488.7A CN201410499488A CN104263684B CN 104263684 B CN104263684 B CN 104263684B CN 201410499488 A CN201410499488 A CN 201410499488A CN 104263684 B CN104263684 B CN 104263684B
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peanut
bacteria suspension
siderophore
series bacillus
arachidis hypogaeae
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CN104263684A (en
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丁延芹
杜秉海
姚良同
刘娣
刘凯
王文铜
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Shandong Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

Abstract

The invention discloses a kind of product siderophore series bacillus, this bacterial strain is Illinois series bacillus (Paenibacillus illinoisensis), in preservation China Committee for Culture Collection of Microorganisms common micro-organisms on August 29 center (CGMCC) in 2014, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number: CGMCC No.9620.Bacterial strain of the present invention can produce siderophore in limit ferrum culture medium.Under potted plant and field condition, with the bacteria suspension producing siderophore bacterial strain of the present invention before peanut seeding after seed soaking and trophophase root irrigation, the melt quality in peanut growth period can be obviously improved in calacareous soil, and Folium Arachidis hypogaeae chlorophyll contents can be increased, strengthen the improving activity of root system of Semen arachidis hypogaeae, promote nitrogen, phosphorus, the accumulation of potassium in peanut plant body, improve biomass, improve peanut quality, improve peanut yield.

Description

A kind of product siderophore series bacillus and application thereof
Technical field
The present invention relates to a kind of product siderophore series bacillus and application thereof.
Background technology
Semen arachidis hypogaeae is the important oil crop of China and industrial crops, and Northern Part of China is the cultivation area that Semen arachidis hypogaeae is maximum One.In recent years, the generation of Semen arachidis hypogaeae iron deficiency disease disease is increasingly severe, and this all causes the biggest damage to yield and the quality of Semen arachidis hypogaeae Evil.At present people prevent and treat Semen arachidis hypogaeae iron deficiency disease disease and mainly rely on cultural control and the method for chemical prevention, but cultural control Method wastes time and energy, and is difficult to the generation of effective controlling disease from root, and chemical prevention and control method is big for environment pollution, and Health is also had certain injury.Therefore, utilize biological method to have prevented and treated the sick generation of Semen arachidis hypogaeae iron deficiency disease It is increasingly becoming focus of concern, and finding the microorganism resource having fine preventive and therapeutic effect to Semen arachidis hypogaeae iron deficiency disease disease is to close Key, wherein produces the screening of siderophore bacterial strain and application is one of the study hotspot of current microbiology worker.
Ferric ion is had efficiently by the class that siderophore is microorganism and Activities of Some Plants synthesizes under low ferrum stressed condition Low-molecular-weight (1-2kDa) organic compound of affinity, it can be taken by force from various water solublity and non-water-soluble compound Walk Fe3+.In recent years, siderophore microorganism biological and ecological methods to prevent plant disease, pests, and erosion in terms of plant and growth-promoting functions are produced increasingly by domestic and international researcher Concern.A lot of achievements in research discuss the mechanism of action that soil microorganism is possible in root system of plant absorbs ferrum, this mechanism Potentially include following several: (1) iron deficiency plant root exudation small molecular organic compounds, such as the compound such as phenols and flavin, These compounds the carbon source as antibacterial and (or) directly as growth of microorganism can change plant rhizosphere microbe Structure of community, and form specific microbial population at plant rhizosphere, this specificity microorganism population will be by secretion Siderophore increases the content of effective ferrum in soil, thus improves the plant absorption to ferrum.(2) some are with root system of plant symbiosis Microorganism also can improve enhancing plant and absorb ferrum, and such as root nodule bacteria (Rhizobium) can be come by noclulation Strengthen the physiological responses that plant iron is coerced；Infect the mycorrhizal fungi of root system by increasing the Nutrient Absorption area of root system of plant and dividing Secrete siderophore to improve the melt quality of plant.
Currently, with respect to producing the pseudomonas of siderophore and fungus report in terms of improving plant iron nutrition and growth-promoting relatively Many, but about series bacillus, the report in terms of the improvement of plant iron nutrition and growth-promoting is not had.
Summary of the invention
It is an object of the invention to provide a kind of product siderophore series bacillus and application, mainly changing at Iron Nutrition of Peanut Application in terms of kind and growth-promoting.
For achieving the above object, the present invention is achieved through the following technical solutions:
A kind of product siderophore series bacillus, this strain classification named Illinois series bacillus (Paenibacillus illinoisensis) YZ29, depositary institution: China Committee for Culture Collection of Microorganisms is the most micro- Bio-Centers (CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on August 29th, 2014, preservation Numbering: CGMCC No.9620.
Bacterium colony and the thalline of this bacterial strain are characterized as: the product siderophore series bacillus of the present invention is at LB plating medium (ferment Female extractum, 5g；Peptone, 10g；Sodium chloride, 10g；Distilled water, 1000mL；Agar, 15-20g；121 DEG C of sterilizing 20min； pH7.0.After cultivating 24h on), bacterium colony is rounded, and milk yellow slightly swells, neat in edge, smooth surface.Thalline is elongated rod shape, greatly Little for 0.5-0.7 × 1.2-2.0 μm, product spore, Gram-positive.
The physiological and biochemical property of this bacterial strain is: Fu-general negative, and citrate utilizes negative, nitrate reduction Negative, D-Glucose fermentation test is negative, mannose ferment negative, and L-arabinose fermentation test is positive, starch Hydrolysis experiment is positive, and gelatin liquefaction test is positive, and catalase test is positive, and oxidase test is negative.
The application in terms of improving Iron Nutrition of Peanut and growth-promoting of the above-mentioned product siderophore series bacillus.
The application process of described product siderophore series bacillus, prepares bacteria suspension, and by above-mentioned bacteria suspension, (concentration is 108cfu·mL-1, bacterium solution volume is for can all seeds of submergence) soak peanut seed 25-35min (preferably 30min) and sow afterwards；Or Person after Semen arachidis hypogaeae final singling and before blooming (or rear) use bacteria suspension pouring root；Or above-mentioned bacteria suspension is soaked peanut seed sowing After, after Semen arachidis hypogaeae final singling and before blooming, (or rear) uses bacteria suspension pouring root.
The concentration of described bacteria suspension is 108cfu·mL-1。
Consumption during pouring root is: every strain 25mL, field test every strain 200mL in pot experiment.
The preparation method of bacteria suspension: the CGMCC9620 that inclined-plane preserves is transferred to equipped with 5-10mL (preferably 10mL) liquid (pot experiment is LB culture medium to culture medium；Field test is bean sprouts medium) test tube in, be placed in constant-temperature table cultivation 12-16h (preferably 12h), temperature: 37 DEG C；Rotating speed: 180-200rpm.The bacterium solution activated is linked into 100-by 1% inoculum concentration In 200mL (preferably 200mL) culture medium, put into shaking table and cultivate 24h, temperature: 37 DEG C；Rotating speed: 180-200rpm (180rpm), so Rear 8000rpm is centrifuged 10min, abandoning supernatant, adds appropriate sterile deionized water, makes bacteria suspension.
LB culture medium: yeast extract, 5g；Peptone, 10g；Sodium chloride, 10g；Distilled water, 1000mL；121 DEG C of sterilizings 20min；pH7.0.
Bean sprouts medium: Semen Glycines Germinatus 10g, sucrose 30g, ammonium sulfate 8g, distilled water, 1000mL；121 DEG C of sterilizing 20min； pH7.0。
A kind of Biological control microbial inoculum, including above-mentioned Illinois series bacillus (Paenibacillus illinoisensis)。
The application in terms of improving Iron Nutrition of Peanut and growth-promoting of the above-mentioned bacteria agent.
Beneficial effect produced by the present invention:
Show after deliberation, potted plant with under field condition, broadcast at Semen arachidis hypogaeae with the bacteria suspension producing siderophore bacterial strain of the present invention After planting front seed soaking and trophophase root irrigation, calacareous soil can be obviously improved the melt quality in peanut growth period, And Folium Arachidis hypogaeae chlorophyll contents can be increased, strengthen the improving activity of root system of Semen arachidis hypogaeae, promote nitrogen, phosphorus, the accumulation of potassium in peanut plant body, carry High biomass, improves peanut quality, improves peanut yield.
The product siderophore series bacillus of the present invention can be obviously improved the Iron status in peanut growth period, promotes Semen arachidis hypogaeae Growth.
This research screening CGMCC9620 bacterial strain under condition of pot and field condition to Iron Nutrition of Peanut and growth-promoting effect Research, the test method used and condition, compared with existing document report, (are much reported closer to realistic application conditions Research pseudomonas and fungus be verify under the conditions of water planting the impact on Semen arachidis hypogaeae rather than in calacareous soil), this Also strongly suggested that the effect in terms of the absorption to Iron Nutrition of Peanut and growth-promoting is clearly.It addition, have no at present about Series bacillus is to the report in terms of the improvement of plant iron nutrition and growth-promoting, and this illustrates the CGMCC9620 bacterial strain that this research is sifted out Enrich the microorganism resource that preventing and treating Semen arachidis hypogaeae iron deficiency disease is sick.Series bacillus be a kind of antibacterial that can form spore it than vacation Zymomonas mobilis is easier in soil existence, is easier to play a role than fungus fast growth.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
A kind of product siderophore series bacillus, this strain classification named Illinois series bacillus (Paenibacillus illinoisensis), depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms Center (CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on August 29th, 2014, preservation is compiled Number: CGMCC No.9620.
CGMCC9620 screening process:
(1) separation and purification
This strain separation screening from Semen arachidis hypogaeae rhizosphere soil obtains.
Pedotheque picks up from hollow (35 ° of 44'N, 116 ° of 75'E) peanut cultivation ground, stockaded village, Yanzhou City of Shandong Province.By Semen arachidis hypogaeae rhizosphere Soil is carefully shaken off in sterile bag, is put in preservation by low temperature box, processes immediately after taking back laboratory.Weigh rhizosphere soil sample Product 10g, puts in 90mL sterilized water, makes Soil Slurry after vibration 30min.Draw 1mL suspension respectively, according to 10 multiple proportions After example gradient dilution, respectively take 10-4、10-5With 10-6Times diluent 200 μ L coats beef extract-peptone flat board (Carnis Bovis seu Bubali cream, 5g； Peptone, 10g；Sodium chloride, 10g；Distilled water, 1000mL；Agar, 15-20g；121 DEG C of sterilizing 20min；PH7.2-7.4) on, It is inverted in 37 DEG C of constant incubators after standing 10min and cultivates about 24h.Select representative colonies on flat board again by three zonings Collimation method purification, microorganism separator after purification is: CGMCC9620, and separator is stored in inclined-plane standby.
(2) CGMCC9620 produces the detection of siderophore performance
Bacterial strain toothpick point is connected on CAS flat board, 37 DEG C of constant incubators are cultivated 2-5d, observe periphery of bacterial colonies and produce Orange-yellow variable color circle person is the bacterial strain that can produce siderophore, if being formed without variable color circle explanation bacterial strain can not produce siderophore.
Prepared by described CAS flat board:
1. take 0.012g CAS (Chromeazuorls, sea, sea, Beijing Fine Chemical Works) to be dissolved in 10mL distilled water, and with The FeCl of 2mL1mmol/L3Solution mixes, and obtains solution a；
2. (Hexadeeyltrimethylammoniumbormide, Beijing Suo Laibao science and technology is limited to take 0.015gHDTMA Company) it is dissolved in 8mL distilled water, obtain solution b；
3. being slowly added in b solution by a solution, abundant mixing i.e. obtains dye liquor c；
4. by 10 × MM9 saline solution (Na2HPO430g, KH2PO41.5g, NaCl 2.5g, NH4Cl 5g, distilled water 500mL) 20mL, piperazine Diethanol sulfonic acid (pipes, Sigma company product) 6.04g add the clean triangular flask filling 150mL distilled water In, after mixing with mass concentration be 50%NaOH regulate pH to 6.8, add agar powder 3.2g, obtain culture medium d；
5. by dye liquor c, culture medium d and 1mmol/L CaCl2、1mmol/L MgSO4·7H2O, mass concentration are 20% Portugal Grape sugar, mass concentration are, after 10% hydrolyzed casein is sterilized separately (115 DEG C, 20min), to be placed in 50 DEG C of water-bath inside holding stand-by；
Measure above-mentioned 1mmol/L CaCl the most respectively20.2mL、1mmol/L MgSO4·7H2O 4mL, mass concentration are 20% glucose 2mL and mass concentration are that 10% hydrolyzed casein 6mL adds in culture medium d, then add dye liquor c along bottle wall, fully Shake up (not producing bubble), obtain blue qualitative detection culture medium.
Then pour in culture dish by every ware 20mL, to be solidified rear standby.
CGMCC9620 amplification culture method:
The CGMCC9620 that inclined-plane preserves is transferred to equipped with 10mL LB liquid medium (yeast extract, 5g；Peptone, 10g；Sodium chloride, 10g；Distilled water, 1000mL；121 DEG C of sterilizing 20min；pH7.0.) test tube in, be placed in constant-temperature table training Support 12h (temperature: 37 DEG C；Rotating speed: 180rpm).The bacterium solution activated is filled 200mL by what 1% inoculum concentration was linked into 1000mL LB liquid medium or bean sprouts medium (Semen Glycines Germinatus 10g, sucrose 30g, ammonium sulfate 8g, distilled water, 1000mL；121 DEG C of sterilizings 20min；pH7.0.) triangular flask in, put into shaking table cultivate 24h (temperature: 37 DEG C；Rotating speed: 180rpm).
The qualification of CGMCC9620
(1) colony characteristics and thalli morphology
Bacterium colony and the thalline of this bacterial strain are characterized as: (yeast extract, 5g on LB plating medium；Peptone, 10g；Chlorination Sodium, 10g；Distilled water, 1000mL；Agar, 15-20g；121 DEG C of sterilizing 20min；pH7.0.) to cultivate bacterium colony after 24h rounded, breast Yellow, slightly swells, neat in edge, smooth surface.Thalline is elongated rod shape, and size is (0.5-0.7) μ m (1.2-2.0) μm, produces Spore, Gram-positive.
(2) physiological and biochemical property
Fu-general negative, citrate utilizes negative, and nitrate reduction test is negative, D-Glucose fermentation examination Testing feminine gender, mannose ferment negative, L-arabinose fermentation test is positive, and the Starch Hydrolysis test positive, gelatin liquefaction tries Testing the positive, catalase test is positive, and oxidase test is negative.
(3) 16S rDNA sequence analysis
16S rDNA gene order, as shown in sequence table.By surveyed 16S rDNA sequence and the sequence in GenBank data base Row comparison, result shows: the bacterial strain of the present invention and Paenibacillus illinoisensis (AB073192) are in one together In minimum branch, its 16S rDNA gene order and the bacterial strain sequence of Paenibacillus illinoisensis (JQ579623) Row similarity is 99%, in conjunction with above-mentioned physiological and biochemical property result, identifies that the bacterial strain of the present invention is Illinois series bacillus (Paenibacillus illinoisensis)。
For clear and definite CGMCC9620 application in terms of the improvement and growth-promoting of Iron Nutrition of Peanut, carry out following test:
Pot experiment and field test
The CGMCC9620 that inclined-plane preserves is transferred to equipped with 10mL fluid medium that (pot experiment is LB culture medium, field Test is for bean sprouts medium) test tube in, be placed in constant-temperature table cultivation 12h (temperature: 37 DEG C；Rotating speed: 180rpm).To live The bacterium solution changed is linked in 200mL culture medium by 1% inoculum concentration, puts into shaking table and cultivates 24h (temperature: 37 DEG C；Rotating speed: 180rpm), then 8000rpm is centrifuged 10min, abandoning supernatant, adds appropriate sterile deionized water, makes concentration and is 108cfu·mL-1Bacteria suspension.
Above-mentioned LB culture medium: yeast extract, 5g；Peptone, 10g；Sodium chloride, 10g；Distilled water, 1000mL；121 DEG C go out Bacterium 20min；pH7.0.
Bean sprouts medium: Semen Glycines Germinatus 10g, sucrose 30g, ammonium sulfate 8g, distilled water, 1000mL；121 DEG C of sterilizing 20min； pH7.0。
Prior to seeding, by peanut seed 108cfu·mL-1Bacteria suspension in soak 30min, take out seed, by surface Water dries in the shade, sowing.Again with 10 after Semen arachidis hypogaeae final singling and before blooming8cfu·mL-1Bacteria suspension use root-pouring method respectively execute bacterium one Secondary, every strain 25mL, field test every strain 200mL in pot experiment.Matched group equivalent sterile deionized water processes.Each at Semen arachidis hypogaeae Period of duration (i.e. Seedling Stage, lower pin phase of blooming, pod phase and full fruit phase) measure peanut leaf active Fe content and chlorophyll content, Improving activity of root system, total nitrogen phosphorus potassium, statistical correlation yield factor during results.Result is as shown in the table.
The chlorophyll content of table 1 each period of duration different disposal group Semen arachidis hypogaeae leaf in pot experiment and the difference of active Fe content Different
C: blank, does not use microbial inoculum；Microbial inoculum prepared by Y:CGMCC9620.Below the average of Y process group in bracket The percentage ratio that numeric representation improves than matched group (C).Lower same.
The chlorophyll content of table 2 each period of duration different disposal group Semen arachidis hypogaeae leaf in field test and the difference of active Fe content Different
Under the conditions of soil iron deficiency, the active Fe content of Semen arachidis hypogaeae leaf can direct reaction soil microorganism supply peanut iron The situation of nutrition.Ferrum is most important to the synthesis of plant chlorophyll, and chlorophyllous synthesis precursor ALA (5-ALA) is in shape Need the participation of ferrum during one-tenth, ALA form chlorophyllous process and be also required to the participation of ferrum.Therefore, peanut leaf active iron Content and chlorophyll content can be with the Iron status of concentrated expression Semen arachidis hypogaeae.Under condition of pot, in four period of duration, The chlorophyll of the Semen arachidis hypogaeae leaf of CGMCC9620 process group and active Fe content all higher than matched group (table 1)；At field condition Under, the leaf chlorophyll of CGMCC9620 process group and active Fe content remain unchanged always than matched group in the whole period of duration of Semen arachidis hypogaeae Height (table 2), thus prove, CGMCC9620 can promote that the melt quality of Semen arachidis hypogaeae absorbs.
Table 3 improving activity of root system difference of Semen arachidis hypogaeae under each period of duration different disposal in pot experiment
TAAR: total absorption area；AAAR: enliven absorption area.
Root system of plant vigor intensity is closely related with the whole vitality of plant, the size of Peanut Root System absorption area and The intensity of improving activity of root system has direct relation with the g and D of Semen arachidis hypogaeae aerial parts.The total absorption area of root system can be the most anti- Reflect Root Absorption moisture, the size of nutrient ability, and active area of root system can reflect improving activity of root system situation.Permissible by table 3 Find out, the total absorption area of Peanut Root System of CGMCC9620 process group and to enliven absorption area all higher than matched group.Therefore, CGMCC9620 has certain help to the raising of Peanut Root System vigor.
Table 4 is each period of duration different disposal full nitrogen of group peanut plant, full phosphorus and the difference of full potassium accumulation in pot experiment
Table 5 is each period of duration different disposal full nitrogen of group peanut plant, full phosphorus and the difference of full potassium accumulation in field test
Nitrogen, phosphorus and potassium are the three big nutrients that plant is required, and in wherein nitrogen and phosphorus are plant, many is important organises The composition of compound, affects metabolic process and the growth promoter of plant, nitrogen or chlorophyllous important composition in many-side Element, the rich scarce of nitrogen has close relationship with leaf Determination of Chlorophyll content；Potassium is the activator of multiple enzyme in plant, in generation Play an important role during thanking, not only can promote photosynthesis, it is also possible to promote nitrogen metabolism, improve plant to the absorption of nitrogen and Utilize.Ferrum is not only ferritin and the required component of Mo-Fe protein in microorganism azotase, and is legume symbiosis One of essential elements of nitrogen fixation.Therefore, the photosynthesis of plant can be along with ferrum element and the raising of nitrogen element absorption ability And strengthen, grow the most vigorous, the ability of plant absorption nutrient is got back enhancing.Found out, at Semen arachidis hypogaeae by table 4 and table 5 In trophophase, nitrogen in the peanut plant body of CGMCC9620 process group, phosphorus, potassium accumulation higher than matched group, explanation The siderophore that CGMCC9620 produces, while promoting Iron Nutrition of Peanut to absorb, also promotes Semen arachidis hypogaeae to nitrogen, phosphorus, the absorption of potassium, Thus promote the growth of Semen arachidis hypogaeae.
Table 6 is the biomass of each growthdevelopmental stage different disposal group and the difference of yield factor when gathering in the crops in field test
Peanut plant Biomass and Pod Yield are the end reactions that can CGMCC9620 promote peanut growth.In field In test, the peanut pod yield increased group that CGMCC9620 processes improve 37.5%, this reports than Dey in 2004 The peanut pod yield increased group that bacterial strain PGPR2 processes improves the result of 28.3% and to get well.This more convincingly demonstrates CGMCC9620 can promote the absorption of Iron Nutrition of Peanut, thus improves peanut yield.
The Semen arachidis hypogaeae protein of different disposal group and the difference of crude fat content when table 7 is gathered in the crops in pot experiment
The Semen arachidis hypogaeae protein of different disposal group and the difference of crude fat content when table 8 is gathered in the crops in field test
Semen arachidis hypogaeae belongs to leguminous plant, is a kind of important oil plant and protein resource.Found out, in pot experiment by table 7 and table 8 With in field test, CGMCC9620 can improve the quality of Semen arachidis hypogaeae.
Although the above-mentioned detailed description of the invention to the present invention is described, but not limit to scope System, one of ordinary skill in the art should be understood that on the basis of technical scheme, and those skilled in the art need not pay Go out various amendments or deformation that creative work can make still within protection scope of the present invention.

Claims (8)

1. one kind is produced siderophore series bacillus, it is characterised in that this strain classification named Illinois series bacillus (Paenibacillus illinoisensis), depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms Center (CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on August 29th, 2014, preservation is compiled Number: CGMCC No.9620.
2. the product siderophore series bacillus application in terms of improving Iron Nutrition of Peanut and growth-promoting described in claim 1.
3. the product siderophore series bacillus application process in terms of improving Iron Nutrition of Peanut and growth-promoting described in claim 1, It is characterized in that, preparation concentration is 108cfu·mL-1Bacteria suspension, broadcasts after above-mentioned bacteria suspension is soaked peanut seed 25-35min Kind, bacterium solution volume is that all seeds of submergence are as the criterion；Or after Semen arachidis hypogaeae final singling, bloom before or Post flowering bacteria suspension pouring root；Or Person be by described bacteria suspension soak peanut seed after planting, after Semen arachidis hypogaeae final singling, bloom before or Post flowering bacteria suspension pouring root.
4. application process as claimed in claim 3, it is characterised in that consumption during pouring root is: potted plant every strain 25mL, field is every Strain 200mL.
5. application process as claimed in claim 3, it is characterised in that above-mentioned bacteria suspension is soaked peanut seed 30min.
6. application process as claimed in claim 3, it is characterised in that the preparation method of described bacteria suspension: inclined-plane is preserved CGMCC9620 is transferred in the test tube equipped with 5-10mL fluid medium, is placed in constant-temperature table cultivation 12-16h, temperature: 37 DEG C, rotating speed: 180-200rpm；The bacterium solution activated is linked in 100-200mL culture medium by 1% inoculum concentration, puts into and shake Bed cultivates 24h, temperature: 37 DEG C, rotating speed: 180-200rpm, and then 8000rpm is centrifuged 10min, abandoning supernatant, adds appropriate Sterile deionized water, make bacteria suspension；Described fluid medium is LB culture medium: yeast extract, 5g, peptone, 10g, chlorine Change sodium, 10g, distilled water, 1000mL, 121 DEG C of sterilizing 20min, pH7.0；Or bean sprouts medium: Semen Glycines Germinatus 10g, sucrose 30g, Ammonium sulfate 8g, distilled water, 1000mL, 121 DEG C of sterilizing 20min, pH7.0.
7. a Biological control microbial inoculum, it is characterised in that include the Illinois series bacillus described in claim 1 (Paenibacillus illinoisensis)。
8. the application in terms of improving Iron Nutrition of Peanut and growth-promoting of the Biological control microbial inoculum described in claim 7.
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