CN109182194A - One plant of Yang Ling rhizobium for promoting coronule flower growth and its cultural method and application - Google Patents
One plant of Yang Ling rhizobium for promoting coronule flower growth and its cultural method and application Download PDFInfo
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Abstract
The Yang Ling rhizobium and its cultural method that promote coronule flower growth the invention discloses one plant and application.The bacterial strain number of the Yang Ling rhizobium is cv8401, is CGMCC No.15532 in the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center.It is demonstrated experimentally that plant dross rate, plant height, hay yield and seed production are dramatically increased than not connecing bacterium control after facet syndrom inoculation Yang Ling rhizobium cv8401 CGMCC No.15532.The present invention has broad application prospects in leguminous plant planting industry.
Description
Technical field
The Yang Ling rhizobium for promoting coronule flower growth the present invention relates to one plant in field of agricultural microorganism and its cultural method
With application.
Background technique
Facet syndrom (Coronilla varia) is the herbaceos perennial of pulse family Coronilla, is grown thickly with crawling, branches and leaves
Dense, resistance is strong, is preferable covering green manure and the valuable high protein forage grass of ruminant.The root system of facet syndrom is sent out
It reaches, except main root lateral root is also very flourishing, string can be laterally walked in soil layer, well developed root system has root nodule, and nitrogen fixing capacity is strong, breeding
Rate is high, and plant is luxuriant, and grass yield is high, and general arable land can mow three times every year, and coverage is big, can quickly form turf, be one
The good plant that conserves water and soil of kind.Facet syndrom adapts to North of Yangtze River and the vast area south of the Yangtze River in China, and in certain model
The Changjiang river is crossed in enclosing.Facet syndrom not only can improve soil with culture fertility, and can beautify the environment, and reduce pollution.
Nitrogen is nutrient important in plant growth, and crops are real dependent on the volume increase obtained of application chemical nitrogen fertilizer
It is to be turned the nitrogen in air as the nitrogen-fixing microorganism acquired by cost, and in soil using consuming the energy and pollution environment on border
The process for turning to the ammonia of plant available is both efficient and environmental sound.Rhizobium (rhizobia) are that one kind is widely present in
Bacterium in soil, they can form symbiosis with leguminous plant, form root by the root or stem of infecting leguminous plant
Nitrogen in air is converted to the high-quality nitrogen-glutamic acid and paddy ammonia phenol that plant available utilizes in the form of homobium by tumor
Amine substance.The research and application of Rhizobium leguminosarum have more than 100 years history, almost all advocate to beans in worldwide
Section crop carries out legume inoculation.Rhizobium and legume symbiosis system be the known strongest system of biological nitrogen fixation ability it
One, annual amount of nitrogen fixation accounts for 65% or more of the total amount of nitrogen fixation of global biology, develops and utilizes leguminous plant and rhizobium symbiotic nitrogen fixation
Effect has a high potential.
Summary of the invention
The technical problem to be solved by the present invention is to how remarkably promote facet syndrom to increase and/or increase production.
In order to solve the above technical problems, the present invention provides one plant of rhizobium.
Rhizobium provided by the present invention are Yang Ling rhizobium (Rhizobium yanglingense), and bacterial strain number is
Cv8401, the bacterial strain were preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on 03 30th, 2018
The heart (abbreviation CGMCC, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), deposit number are CGMCC No.15532, with
It is referred to as Yang Ling rhizobium cv8401 down.
Yang Ling rhizobium cv8401 has 16s rDNA sequence shown in sequence 1 in sequence table.
The culture of Yang Ling rhizobium cv8401 also belongs to protection scope of the present invention.
The culture of Yang Ling rhizobium cv8401 provided by the present invention can be by Yang Ling rhizobium cv8401 in microorganism
The all substances in culture vessel cultivated in culture medium.The culture includes the Yang Ling rhizobium cv8401 and institute
State the metabolin of Yang Ling rhizobium cv8401.
In the culture of above-mentioned Yang Ling rhizobium cv8401, the microbiological culture media can be trained for solid medium or liquid
Support base.
In order to solve the above technical problems, the present invention also provides the microbial inoculums for promoting leguminous plant growth and/or volume increase.
It is provided by the present invention promote leguminous plant growth and/or volume increase microbial inoculum, containing Yang Ling rhizobium cv8401 or/
With the culture of Yang Ling rhizobium cv8401.
In above-mentioned microbial inoculum, promotion leguminous plant growth and/or volume increase concretely improve leguminous plant plant height and/or
It improves leguminous plant hay yield and/or improves seeds of leguminous plant yield.
The active constituent of above-mentioned microbial inoculum can be the culture of Yang Ling rhizobium cv8401 or/and Yang Ling rhizobium cv8401,
The active constituent of above-mentioned microbial inoculum can also contain other biological ingredient or abiotic component, the other active components ability of above-mentioned microbial inoculum
Field technique personnel can be according to microbial inoculum to leguminous plant plant height and/or leguminous plant hay yield and/or seeds of leguminous plant yield
Facilitation effect determines.
The microbial inoculum may also include carrier.The carrier can be solid carrier or liquid-carrier.The solid carrier is mine
Object material, biomaterial；The mineral material can for turf, clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica and
At least one of diatomite；The biomaterial be the stalks of all kinds of crops, loose shell, straw, peanut shell, corn flour, bean powder,
At least one of starch, turf and excrement of animal；The liquid-carrier can be water；In the microbial inoculum, Yang Ling rhizobium
The culture of cv8401 or/and Yang Ling rhizobium cv8401 can be trained with the fermentation liquid of the living cells, living cells that are cultured, cell
Support the filtrate of object or the form presence of cell and the mixture of filtrate.The dosage form of the microbial inoculum can be a variety of dosage forms, such as liquor, cream
Agent, suspending agent, pulvis, granule, wettable powder or water dispersible granules.
As needed, surfactant (such as polysorbas20, Tween 80), adhesive, stabilization can be also added in the microbial inoculum
Agent (such as antioxidant), pH adjusting agent.
The culture of Yang Ling rhizobium cv8401, Yang Ling rhizobium cv8401 promotes leguminous plant growth and/or volume increase
Microbial inoculum preparation promote leguminous plant growth and/or volume increase product in application and promote leguminous plant growth and/or increase
Application in production all belongs to the scope of protection of the present invention.
In above-mentioned application, promotion leguminous plant growth and/or volume increase concretely improve leguminous plant plant height and/or
It improves leguminous plant hay yield and/or improves seeds of leguminous plant yield.
In above-mentioned application, it is described promote leguminous plant growth and/or volume increase product can for promote leguminous plant growth and/
Or the bio-feritlizer of the microbial inoculum increased production or the microbial inoculum for containing the promotion leguminous plant growth and/or volume increase.
In the application, the leguminous plant can be Coronilla plant, such as facet syndrom.
In order to solve the above technical problems, the present invention also provides a kind of methods for cultivating Yang Ling rhizobium cv8401.
The method of culture Yang Ling rhizobium cv8401 provided by the present invention, including in the culture medium for cultivating rhizobium
The step of middle culture Yang Ling rhizobium cv8401.
The culture medium for cultivating rhizobium can be prepared as follows: glycerine 3-5ml, mannitol 2-5g,
Yeast extract 0.8-1g, K2HPO40.5-1g, anhydrous MgSO40.1-0.2g, CaSO4·2H2O 0.1-0.2g, NaCl 0.1-
The 0.2g, (NH that mass content is 1%4)6Mo7O24·4H2O aqueous solution 1-1.5ml, the H that mass content is 1%3BO3Aqueous solution
1-1.5ml, agar 20g are settled to 1000ml with water, and tune pH value is 6.8-7.0, and sterilizing obtains the culture for cultivating rhizobium
Base.
In order to solve the above technical problems, the present invention also provides a kind of sides for preparing Yang Ling rhizobium cv8401 microbial inoculum
Method.
The method provided by the present invention for preparing Yang Ling rhizobium cv8401 microbial inoculum, including by Yang Ling rhizobium cv8401
And/or the step of ingredient of the culture of Yang Ling rhizobium cv8401 as the microbial inoculum.
It is demonstrated experimentally that after Yang Ling rhizobium cv8401 is inoculated with facet syndrom, dross rate, plant height, plant hay weight
Dramatically increase with seed production than not connecing bacterium control: dross rate increases by 326.47% than not connecing bacterium control, plant height ratio
Bacterium control is not connect and increases by 106.03%, and plant hay yield increases by 83.28% than not connecing bacterium control, and seed production ratio does not connect bacterium pair
According to increase by 75.44%.Present invention practicability with higher and replicability, are expected to develop into Yang Ling rhizobium application field
In a new excellent combination technique.The present invention has broad application prospects in facet syndrom planting industry.
Preservation explanation
Strain name: Yang Ling rhizobium (Rhizobium yanglingense)
Strain number: cv8401
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on 03 30th, 2018
Collection is registered on the books number: CGMCC No.15532
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified
Conventional method.The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Rhizobium (Rhizobium sp.) ACCC19628 in following embodiments was concealed in China on 2 1st, 1987
Microbiological Culture Collection administration committee agricultural microorganism center (abbreviation ACCC, address: ZhongGuanCun south Street, Haidian District, BeiJing City
No. 12, INST OF AGRICULTURAL RESOURCES, postcode 100081), the public can be from from the collection day
China Committee for Culture Collection of Microorganisms agricultural microorganism center obtains the bacterial strain.Hereinafter referred to as rhizobium ACCC19628.
The preparation method of solid medium in following embodiments is as follows: glycerine 5ml, mannitol 5g, yeast extract 1g,
K2HPO40.5g, anhydrous MgSO40.2g, CaSO4·2H2O 0.2g, NaCl the 0.1g, (NH that mass content is 1%4)6Mo7O24·4H2O aqueous solution 1ml, the H that mass content is 1%3BO3Aqueous solution 1ml, agar 20g are settled to 1000ml with water,
Tune pH value is 6.8-7.0,121 DEG C of sterilizing 30min.
The preparation method of fluid nutrient medium in following embodiments is as follows: glycerine 5ml, mannitol 5g, yeast extract 1g,
K2HPO40.5g, anhydrous MgSO40.2g, CaSO4·2H2O 0.2g, NaCl the 0.1g, (NH that mass content is 1%4)6Mo7O24·4H2O solution 1ml, the H that mass content is 1%3BO3Aqueous solution 1ml is settled to 1000ml with water, and tune pH value is
6.8-7.0 121 DEG C of sterilizing 30min.
The separation of embodiment 1, Yang Ling rhizobium (Rhizobium yanglingense) cv8401 CGMCC No.15532
And identification
1, the separation of bacterial strain
It acquires facet syndrom root nodule within the border from Shaanxi Province, (takes mannitol 10g, yeast extract 1g, dipotassium hydrogen phosphate with plate
0.5g, calcium sulfate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.1g, the ammonium molybdate aqueous solution 1ml and quality that mass content is 1% contain
Amount is 1% boric acid aqueous solution 1ml, 0.5% Congo red 1ml, and 20g agar is settled to 1L, pH value 6.8-7.0 with water) scribing line
Isolated bacterial strain cv8401.
2, the identification of bacterial strain
2.1, Morphological Identification
Logarithmic growth phase will be in, and bacterium colony size is stablized, above-mentioned steps 1 separate and the bacterial strain cv8401 that purifies into
Row single colonie state description, main includes size, color, transparency, wettability, the bacterium colony surface state, colony edge of bacterium colony
State.On the other hand, to the bacterial strain cv8401 for being in logarithmic growth phase, optical microphotograph sem observation thallus is used after smear staining
Form.
The result shows that bacterial strain cv8401 thallus is short and small rod-shaped, size is 0.5~0.9 micron × 1.2~3.0 microns, not
It is different with the thalli morphology of culture basal growth.Contain Poly-β-hydroxybutyric Acid salt particle.Gram-negative, no gemma have flagellum,
Movement, it is aerobic.25~30 DEG C of optimum growth temperature, optimal pH 6.0~9.0.Bacterium colony is rounded, neat in edge, protrusion, and colourless half
Transparent or light milky white, it is sticky.Color is not inhaled in Congo red culture, abundant extracellular viscous being often accompanied by containing the growth-gen on sugar culture-medium
Liquid.
2.2,16S rDNA sequence homology analysis
Using the 16S rDNA segment of the resulting bacterial strain cv8401 of colony polymerase chain reaction (PCR) method amplification step 1, related reagent is by full formula
King Company provides.To 16S rRNA genetic fragment expand and cloning and sequencing the result shows that, the 16S of bacterial strain cv8401
RDNA has the nucleotide sequence of sequence 1 in sequence table.The 16S rDNA and Yang Ling rhizobium Rhizobium of bacterial strain cv8401
The similitude of the 16S rRNA gene (Sequence ID:JF273845.1) of yanglingense is up to 100%.
2.3, physiological and biochemical property is identified
With reference to " common bacteria system identification handbook " (east show pearl, wonderful Beijing English common bacteria system identification handbook of Cai: section
Publishing house, 2011.) and " Microbiology Experiment " (Beijing Shen Ping, Fan Xiurong, Li Guang force Microbiology Experiment (third edition):
Higher Education Publishing House, 1999.) " Berger bacterial identification manual " the 8th edition (volume science such as the Buchanan R.E., N.E. Ji Bensi
Publishing house, 1984.) physiological and biochemical property of measurement bacterial strain cv8401.The result shows that bacterial strain cv8401 is chmosynthetic heterotrophs, it can benefit
Use the salt of multiple kinds of carbohydrate and organic acid as carbon source, as can using glucose, lactose, D-ribose, D-arabinose,
Mannitol, maltose, D- galactolipin, fructose, trehalose and inositol；Peptone, yeast juice, glutamic acid, ammonium salt, nitric acid can be utilized
Salt and most amino acid can be used as nitrogen source；The Congo red micro- suction color of suction colour response；Litmus milk reaction produces acid, there is serum ring；BTB is produced
Acid；It is unable to hydrolyzed casein；3- ketone group lactose is not utilized；Acid is produced from mannitol；It is abundant being often accompanied by containing growth on sugar culture-medium
Extracellular mucus；Precipitating is not generated in calcium glycerophosphate culture medium.
In view of above-mentioned form, analysis of physio biochemical characteristics and 16s rDNA sequence homology analysis as a result, step 1 is separated
It purifies obtained bacterial strain cv8401 and is accredited as Yang Ling rhizobium (Rhizobium yanglingense).The Yang Ling rhizobium
(Rhizobium yanglingense) cv8401 was preserved in Chinese microorganism strain preservation management on 03 30th, 2018
Committee's common micro-organisms center (abbreviation CGMCC, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), deposit number
For CGMCC No.15532, hereinafter referred to as Yang Ling rhizobium cv8401.
Embodiment 2, promote coronule flower growth Yang Ling rhizobium cv8401 microbial inoculum preparation
1, the inclined-plane culture of Yang Ling rhizobium cv8401
Yang Ling rhizobium cv8401 is inoculated in solid medium and carries out inclined-plane culture, cultivates 72h at 28 DEG C, is obtained tiltedly
The Yang Ling rhizobium cv8401 of face culture.
2, the activation of strain
The Yang Ling rhizobium cv8401 of picking step 1 inclined-plane culture, is inoculated in 500ml fluid nutrient medium, 28
60h is cultivated at DEG C, obtains Yang Ling rhizobium cv8401 bacterium solution.
3, seed tank culture
The Yang Ling rhizobium cv8401 bacterium solution for taking 6L step 2, is accessed in 100L seeding tank (containing 60L Liquid Culture
Base) seed culture is carried out, shaken cultivation 60h is cultivated at 28 DEG C, 150rpm, obtains Yang Ling rhizobium cv8401 seed liquor.
4, fermentation tank culture
(it will contain in the Yang Ling rhizobium cv8401 seed liquor access 1000L fermentor of step 3 in 10% ratio (volume ratio)
Have 600L fluid nutrient medium) fermented and cultured is carried out, the shaken cultivation 60h at 28 DEG C, 150rpm obtains Yang Ling rhizobium cv8401
Fermentation liquid, the content of cv8401 is 2 × 10 in the Yang Ling root nodule fermented liquid9cfu/mL。
5, the preparation of Yang Ling rhizobium cv8401 microbial inoculum
It crushes, sieves with 100 mesh sieve after turf is spontaneously dried, be then 6.8 with limewash tune pH value, go out at 121 DEG C
Bacterium 60min obtains sterile turf.
The Yang Ling rhizobium cv8401 fermentation liquid of step 4 is uniformly mixed with the sterile turf, then is increased under the conditions of 28 DEG C
Culture 48h is grown, Yang Ling rhizobium cv8401 microbial inoculum, packing storage are obtained.Qualified, Yang Ling rhizobium cv8401 through detection finished product
Cv8401 bacterium number content in microbial inoculum is 2.0 × 108cfu/g。
Embodiment 3, Yang Ling rhizobium (Rhizobium yanglingense) cv8401 CGMCC No.15532 and root nodule
The Piglet s colibacillosis of bacterium ACCC19628 inoculation facet syndrom
The rhizobium ACCC19628 isolated from the domestic facet syndrom root nodule in Shaanxi Province, China using the applicant as
Control carries out following tests:
One, the preparation of rhizobium ACCC19628 microbial inoculum
1, inclined-plane culture
Rhizobium ACCC19628 is inoculated in solid medium and carries out inclined-plane culture, cultivates 72h at 28 DEG C.
2, the activation of strain
The rhizobium ACCC19628 of picking step 1 inclined-plane culture is inoculated in the fluid nutrient medium of 500mL, at 28 DEG C
60h is cultivated, rhizobium ACCC19628 bacterium solution is obtained.
3, seed tank culture
The rhizobium ACCC19628 bacterium solution for taking 6L step 2, is accessed in 100L seeding tank (containing 60L Liquid Culture
Base) seed culture is carried out, shaken cultivation 60h is cultivated at 30 DEG C, 120rpm, obtains rhizobium ACCC19628 seed liquor.
4, fermentation tank culture
(it will contain in the rhizobium ACCC19628 seed liquor access 1000L fermentor of step 3 in 10% ratio (volume ratio)
Have 600L fluid nutrient medium) fermented and cultured is carried out, the shaken cultivation 60h at 28 DEG C, 150rpm obtains rhizobium ACCC19628
Fermentation liquid.
5, the preparation of Rhizobial strain (Rhizobium sp.) ACCC19628 microbial inoculum
It crushes, sieves with 100 mesh sieve after turf is spontaneously dried, be then 6.8 with limewash tune pH value, go out at 121 DEG C
Bacterium 60min obtains sterile turf.
The rhizobium ACCC19628 fermentation liquid of step 4 and the sterile turf are mixed, then Multiplying culture under the conditions of 28 DEG C
48h obtains rhizobium ACCC19628 microbial inoculum, packing storage.It is qualified through detection finished product, in the rhizobium ACCC19628 microbial inoculum
Living bacteria count content is 2.0 × 108cfu/g。
Two, promote the field efficacy experiment of coronule flower growth
Test site is in Tongliao City, Inner Mongolia Autonomous Region.This test uses RANDOMIZED BLOCK DESIGN, is randomly provided three processing
Area, each treatment region set three cells and repeat.Three treatment regions are respectively Bu Jiejun control treatment area, the processing of cv8401 microbial inoculum
Area, ACCC19628 microbial inoculum treatment region.Each cell size is 4 × 5m2, soil pH value 7.6.
Yang Ling rhizobium cv8401 microbial inoculum prepared by embodiment 2 is mixed with coronule flower seed according to the mass ratio of 1:10,
It mixes thoroughly, obtained seed is known as cv8401- coronule flower seed.
Rhizobium ACCC19628 microbial inoculum prepared by step 1 is mixed with coronule flower seed according to the mass ratio of 1:10, is mixed
Even, obtained seed is known as ACCC19628- coronule flower seed.
Sterile turf is mixed with coronule flower seed according to the mass ratio of 1:10, is mixed thoroughly, obtained seed is known as without Juncao
Charcoal-coronule flower seed.
Above-mentioned seed is uniformly seeded into soil according to Quarter Design, and type of seeding is drilling, line-spacing 10cm.It is above-mentioned each
For treatment region in addition to the seed sowed is different, other conditions are identical.Wherein, the seed of Bu Jiejun control treatment area sowing is
Sterile turf-coronule flower seed；The seed of cv8401 microbial inoculum treatment region sowing is cv8401- coronule flower seed；ACCC19628 bacterium
The seed of agent treatment region sowing is ACCC19628- coronule flower seed.
When sowing current year seed maturation and when second year seed maturation, each under ground portion dross rate is recorded respectively and (is referred to as tied
Ratio of outflow), aerial part plant height, hay yield and seed production, calculate the average value of each group, obtain 2 years average knots
Ratio of outflow, aerial part plant average height (abbreviation plant average height), average hay yield and average seed production.
The test result of table 1, Yang Ling rhizobium cv8401 microbial inoculum, rhizobium ACCC19628 microbial inoculum inoculation facet syndrom
The results are shown in Table 1, shows:
1, the facet syndrom of cv8401 microbial inoculum treatment region is compared with the facet syndrom in Bu Jiejun control treatment area, 2 years average knots
The average hay yield that the average plant height that ratio of outflow increases by 326.47%, two year increases by 106.03%, two year increases
83.28%, 2 years average seed productions increase by 75.44%.
2, the facet syndrom of cv8401 microbial inoculum treatment region is compared with the facet syndrom of ACCC19628 microbial inoculum treatment region, 2 years flat
The hay yield that the average plant height that equal dross rate increases by 81.25%, two year increases by 46.59%, two year increases by 48.40%,
2 years average seed productions increase by 42.57%.
The above results show that Yang Ling rhizobium cv8401 of the invention to facet syndrom, has more than rhizobium ACCC19628
It is significant to promote growth and effect of increasing production.
The present invention is had been described in detail above.To those skilled in the art, do not depart from spirit of the invention and
Range, and without carrying out under unnecessary experimental conditions, can synchronization parameters, concentration and under the conditions of, it is real in a wider range
Apply the present invention.Although The present invention gives particular embodiments, it is understood that, the present invention can be improved further.
In short, pressing the principle of the present invention, the application is intended to include any change, purposes or improvement of the present invention, including departing from this Shen
Please in the open scope, and the change carried out with routine techniques known in the art.By the range of following attached claims,
It can carry out the application of some essential characteristics.
<110>INST OF AGRICULTURAL RESOURCES
<120>one plants of Yang Ling rhizobium for promoting coronule flower growth and its cultural method and application
<130> GNCFH182036
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1372
<212> DNA
<213>Yang Ling rhizobium (Rhizobium yanglingense)
<400> 1
agggggcgac tacacatgca agtcgagcgc cccgcaaggg gagcggcaga cgggtgagta 60
acgcgtggga acgtaccctt tactacggaa taacgcaggg aaacttgtgc taataccgta 120
tgtgcccttc gggggaaaga tttatcggta agggatcggc ccgcgttgga ttagctagtt 180
ggtggggtaa aggcctacca aggcgacgat ccatagctgg tctgagagga tgatcagcca 240
cattgggact gagacacggc ccaaactcct acgggaggca gcagtgggga atattggaca 300
atgggcgcaa gcctgatcca gccatgccgc gtgagtgatg aaggccctag ggttgtaaag 360
ctctttcacc ggagaagata atgacggtat ccggagaaga agccccggct aacttcgtgc 420
cagcagccgc ggtaatacga agggggctag cgttgttcgg aattactggg cgtaaagcgc 480
acgtaggcgg acatttaagt caggggtgaa atcccagagc tcaactctgg aactgccttt 540
gatactgggt gtctggagta tggaagaggt gagtggaatt ccgagtgtag aggtgaaatt 600
cgtagatatt cggaggaaca ccagtggcga aggcggctca ctggtccatt actgacgctg 660
aggtgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg 720
atgaatgtta gccgtcggca agtttacttg tcggtggcgc agctaacgca ttaaacattc 780
cgcctgggga gtacggtcgc aagattaaaa ctcaaaggaa ttgacggggg cccgcacaag 840
cggtggagca tgtggtttaa ttcgaagcaa cgcgcagaac cttaccagcc cttgacatgc 900
ccggctacct acagagatgt agggttccct tcggggaccg ggacacaggt gctgcatggc 960
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aaccctcgcc 1020
cttagttgcc agcattcagt tgggcactct aaggggactg ccggtgataa gccgagagga 1080
aggtggggat gacgtcaagt cctcatggcc cttacgggct gggctacaca cgtgctacaa 1140
tggtggtgac agtgggcagc gagcacgcga gtgtgagcta atctccaaaa gccatctcag 1200
ttcggattgc actctgcaac tcgagtgcat gaagttggaa tcgctagtaa tcgcggatca 1260
gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt 1320
tggttttacc cgaaggtagt gcgctaaccg caaggaggca gctaacctcg ag 1372
Claims (10)
1. Yang Ling rhizobium, bacterial strain number is cv8401, in China Committee for Culture Collection of Microorganisms's common micro-organisms
The deposit number at center is CGMCC No.15532.
2. the culture of Yang Ling rhizobium described in claim 1 is to train Yang Ling rhizobium described in claim 1 in microorganism
Support the substance in the culture vessel cultivated in base.
3. promoting the microbial inoculum of leguminous plant growth and/or volume increase, it is characterised in that: the microbial inoculum contains described in claim 1
Yang Ling rhizobium or/and culture as claimed in claim 2.
4. microbial inoculum according to claim 3, it is characterised in that: the leguminous plant is Coronilla plant.
5. Yang Ling rhizobium described in claim 1, culture as claimed in claim 2 or microbial inoculum as claimed in claim 3 exist
Preparation promotes the application in the product of leguminous plant growth and/or volume increase.
6. application according to claim 5, it is characterised in that: the leguminous plant is Coronilla plant.
7. Yang Ling rhizobium described in claim 1, culture as claimed in claim 2 or microbial inoculum as claimed in claim 3 exist
Promote the application in leguminous plant growth and/or volume increase.
8. application according to claim 7, it is characterised in that: the leguminous plant is Coronilla plant.
9. the method for preparing Yang Ling rhizobium described in claim 1, including by Yang Ling rhizobium described in claim 1 with
The step of being cultivated in the culture medium for cultivating rhizobium.
10. the method for preparing the microbial inoculum of claim 3 or 4, including by Yang Ling rhizobium described in claim 1 and/or power
Benefit require 2 described in component of the culture as the microbial inoculum the step of.
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| CN115287229A (en) * | 2022-08-04 | 2022-11-04 | 兰州大学 | Rhizobium capable of promoting growth, nodulation and nitrogen fixation of alfalfa beans and application of rhizobium |
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| CN115287229A (en) * | 2022-08-04 | 2022-11-04 | 兰州大学 | Rhizobium capable of promoting growth, nodulation and nitrogen fixation of alfalfa beans and application of rhizobium |
| CN115287229B (en) * | 2022-08-04 | 2023-05-12 | 兰州大学 | Rhizobium capable of promoting growth of alfalfa beans and nodulation and nitrogen fixation and application thereof |
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