CN106007958A - Disease-resistant organic fertilizer microorganism inoculant - Google Patents
Disease-resistant organic fertilizer microorganism inoculant Download PDFInfo
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- CN106007958A CN106007958A CN201610342352.4A CN201610342352A CN106007958A CN 106007958 A CN106007958 A CN 106007958A CN 201610342352 A CN201610342352 A CN 201610342352A CN 106007958 A CN106007958 A CN 106007958A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/70—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting wettability, e.g. drying agents
Abstract
The invention relates to a disease-resistant organic fertilizer microorganism inoculant. The disease-resistant organic fertilizer microorganism inoculant is prepared from the following components in parts by weight: 5 to 10 parts of bacillus thuringiensis, 5 to 10 parts of bacillus amyloliquefaciens, 5 to 10 parts of streptomyces microflavus, 3 to 5 parts of thermophilic sporotrichum, 3 to 5 parts of pseudomonas chlororaphis, 20 to 40 parts of modified zeolite powder, 1.5 to 5 parts of fulvic acid potassium powder, 0.5 to 5 parts of sodium alga acid, 0.5 to 1 parts of water-retaining agent, 1 to 3 parts of polyaspartic acid, 3 to 6 parts of corn starch, 0.5 to 3 parts of tea saponin, 0.5 to 2 parts of complex enzyme preparation, 1 to 5 parts of sodium lignin sulfonate, 0.5 to 1 parts of citric acid, and 0.5 to 1 parts of sodium hydrogen sulfite. The disease-resistant organic fertilizer microorganism inoculant has the advantages that the disease-resistant property and immunity of crops are improved, the soil is loosened, the ecological environment is improved, the pesticide residue and macromolecule organic matter are effectively degraded, and the output and quality of crops are improved.
Description
Technical field
The invention belongs to microbial manure, relate to a kind of disease-resistant organic fertilizer fermenting agent.
Background technology
Chemical fertilizer is one of principal element improving grain yield, and the most too much and irrational using causes soil plate
Knot, soil fertility decline, disease is serious and quality declines.On the other hand, along with science and technology and economic development, people are to life
The requirement of level is also improving constantly, and agricultural product propose variation, high quality, innoxious demand, green safety food
Focus and the fashion of consumption are become.Improve environment, develop eco-agriculture, in the urgent need to improving planting technology, thoroughly change with
Toward relying on a large amount of use fertilizer and pesticide to improve crop yield, produce the practice of a large amount low-quality product, strictly limit pesticide, change
Fertile usage amount.Microbial manure product has improvement soil because of it, reduces the pesticide residues of crop products, stimulates crop
Growth and the raising multi-functional such as crop quality and applied more and more widely, become and develop eco-agriculture, produce green
One of main fertilising kind of food, application area expands year by year, demonstrates good development prospect.
At present, due to a large amount of uses of chemical pesticides various in agricultural production, number of types of pest and disease damage is to this kind of
Medicine creates serious drug resistance, causes prevention effect constantly to reduce.It is reported, the pest species of ten Nian Lai China outburst
Double above.Meanwhile, some pesticide remain in soil, plant and water body to some extent, cause health
Potential threat.When China's many agricultural byproducts export to foreign countries, usually meet with external " green wall because pesticide residues exceed standard
Build " to close down, annual loss is up to 10,000,000,000 dollars, causes the extensive concern of the whole society.Pesticide residues and pathogenic bacteria Drug resistance
It is on the rise Deng series of problems, is the bottleneck restricting the road that agricultural industry turns to Green Development, forces people in science as far as possible
While applied chemistry pesticide, reinforcement Variety Disease-resistance Journal of Sex Research, also constantly seeking new prophylactico-therapeutic measures.Biological control is as planting
One important measures of thing plant disease management system have its inherent advantages, and Biological control environment is harmonious, pollution-free, noresidue, make
Complicated by mechanism, it is not likely to produce Drug resistance, meets the market demand to green agricultural product, therefore develop environmental protection, efficiently biology
Pesticide is significant.
Summary of the invention
The present invention relates to a kind of disease-resistant organic fertilizer fermenting agent, this disease-resistant organic fertilizer fermenting agent can strengthen crops and resist
Characteristic of disease and immunity, it is possible to chesson, improve the ecological environment, can effectively degrading pesticide residues and larger molecular organics, and
Yield and the quality of crops can be improved.
Specifically, the present invention relates to a kind of disease-resistant organic fertilizer fermenting agent, be made up of the component of following weight portion: Su Yun
Gold bacillus 5-10 part, bacillus amyloliquefaciens 5-10 part, streptomyces microflavus 5-10 part, sporotrichum thermophile 3-5 part, green pin vacation unit cell
Bacterium 3-5 part, modified zeolite powder 20-40 part, potassium fulvic acid powder 1.5-5 part, sodium alginate 0.5-5 part, water-retaining agent 0.5-1 part is poly-
Aspartic acid 1-3 part, corn starch 3-6 part, tea saponin 0.5-3 part, compound enzymic preparation 0.5-2 part, sodium lignin sulfonate 1-5
Part, citric acid 0.5-1 part, sodium sulfite 0.5-1 part.
In one preferred embodiment of the invention, described disease-resistant organic fertilizer fermenting agent is by the component of following weight portion
Make: Bacillus thuringiensis 6 parts, bacillus amyloliquefaciens 6 parts, streptomyces microflavus 8 parts, sporotrichum thermophile 4 parts, Pseudomonas chlororaphis
4 parts, modified zeolite powder 30 parts, potassium fulvic acid powder 4 parts, sodium alginate 3 parts, water-retaining agent 0.6 part, poly-aspartate 2 parts, Semen Maydis forms sediment
5 parts of powder, tea saponin 2 parts, compound enzymic preparation 1 part, sodium lignin sulfonate 3 parts, citric acid 0.7 part, sodium sulfite 0.8 part.
In one preferred embodiment of the invention, described disease-resistant organic fertilizer fermenting agent is by the component of following weight portion
Make: Bacillus thuringiensis 5 parts, bacillus amyloliquefaciens 5 parts, streptomyces microflavus 5 parts, sporotrichum thermophile 3 parts, Pseudomonas chlororaphis
3 parts, modified zeolite powder 20 parts, potassium fulvic acid powder 1.5 parts, sodium alginate 0.5 part, water-retaining agent 0.5 part, poly-aspartate 1 part, beautiful
Rice starch 3 parts, tea saponin 0.5 part, compound enzymic preparation 0.5 part, sodium lignin sulfonate 1 part, citric acid 0.5 part, sodium sulfite
0.5 part.
In one preferred embodiment of the invention, described disease-resistant organic fertilizer fermenting agent is by the component of following weight portion
Make: Bacillus thuringiensis 10 parts, bacillus amyloliquefaciens 10 parts, streptomyces microflavus 10 parts, sporotrichum thermophile 5 parts, green pin vacation list
Born of the same parents bacterium 5 parts, modified zeolite powder 40 parts, potassium fulvic acid powder 5 parts, sodium alginate 5 parts, water-retaining agent 1 part, poly-aspartate 3 parts, Semen Maydis
Starch 6 parts, tea saponin 3 parts, compound enzymic preparation 2 parts, sodium lignin sulfonate 5 parts, citric acid 1 part, sodium sulfite 1 part.
In one preferred embodiment of the invention, described water-retaining agent is polyacrylamide and the polyacrylic acid of equivalent
Sodium.
In one preferred embodiment of the invention, described compound enzymic preparation is cellulase, amylase and lignin
Enzyme, the weight ratio of three is 3:1:1.
In one preferred embodiment of the invention, the preparation process of described modified zeolite powder includes: by natural boiling
Stone powder adds stirring 3-6h in concentrated nitric acid solution, filters, and is dried；Then zeolite uses syllogic gradient calcine, specifically calcines
Operation is: (a) carries out calcining 30-60min at 500 DEG C；B () is warmed up to 800 DEG C with the heating rate of 8-12 DEG C/min, and
Insulation calcining 30-60min at a temperature of Gai；C () is warmed up to 1200 DEG C with the heating rate of 5-8 DEG C/min, and protect at such a temperature
Temperature calcining 30-60min, after calcining terminates, naturally cools to room temperature；Zeolite is placed in the ammonium nitrate solution of 1mol/L, heating
To 80-100 DEG C, continuously stirred reaction 5-8h, reaction is filtered after terminating, is washed, is dried, carries out micronizing, obtain modified boiling
Stone powder.
In one preferred embodiment of the invention, described disease-resistant organic fertilizer fermenting agent is prepared as granulated
Shape, or further granule is loaded in capsule.
Another aspect of the present invention relates to the preparation method of a kind of disease-resistant organic fertilizer fermenting agent, it is characterised in that include as
Lower step:
(1) each component raw material is weighed by described weight standby；
(2) first by load weighted Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, green pin vacation unit cell
Send into after bacterium, poly-aspartate, tea saponin, citric acid, sodium sulfite mix homogeneously in granulating disc or rotary drum granulator,
And coordinate and add appropriate steam pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by modified zeolite powder, potassium fulvic acid powder, sodium alginate, water-retaining agent, compound enzymic preparation, sodium lignin sulfonate at batch mixing
In machine after mixing, add granule and the corn starch of preparation in step (2), send into granulating disc after mix homogeneously or rotary drum is made
In grain machine, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, to obtain final product.
Third aspect present invention relates to the using method of described disease-resistant organic fertilizer fermenting agent, including by fermenting agent
10-15 part, bagasse 80-120 part, Pericarppium arachidis hypogaeae 30-50 part, bean cake 30-50 part, chicken manure 30-50 part and water 30-50 part are at room temperature
Under carry out compost fermentation, fermentation time 10-15 days, drying and crushing obtains disease-resistant organic fertilizer, and part is weight portion；By described fertilizer
It is applied in soil.
Bacillus thuringiensis (Bacillus thuringiensis is called for short Bt), can produce two big toxoids, i.e. endotoxin
(parasporal crystal) and extracellular toxin, make insect stop taking food, and last insect is because of hungry and dead, and extracellular toxin effect is slow, is sloughing off
Skin and abnormal time effect substantially, the two is the peak period of RNA synthesis period, and extracellular toxin can suppress the RNA depending on DNA to be polymerized
Enzyme.This medicine effect is slow, and after pests about 2 days just can take effect, about 1 day lasting period.To Lepidoptera, coleoptera, Diptera,
The insecticide such as Hymenoptera, Homoptera, and the arthropod such as animals and plants nematicide, acarian has specific cytotoxicity, and to non-
The most therefore, Bt insecticide has single-minded, efficiently with to advantages such as person poultry safeties target organism safety.
Described bacillus amyloliquefaciens can produce multiple α-amylase and protease, with bacillus subtilis in form, training
Support feature and physio-biochemical characteristics aspect is closely similar；Belonging to facultative anaerobe, bacterium colony is in LB culture medium and beef extract-peptone
In faint yellow opaque colony, rough surface in culture medium, having protuberance, edge is irregular, the most chromogenic in multiple culture medium
Element；Mycoderma is had to be formed when liquid culture is static；Gram’s staining is positive, shaft-like, can form endogenous spore, ovalize, and two
Holding blunt circle, sporangium is not expanded, and middle life is raw to time end, has mobility.Antagonistic protein class, lipopeptide antibiotic etc. can be produced many
Planting antibacterial substance, the antibacterial substance of secretion can destroy the structure of harmful pathogen, suppresses growth of pathogenic bacteria, has extensively suppression
Fungus and the function of antibacterial, and can degrade high starch and the confession plant utilization of height coarse-fibred macromolecular substances.
Streptomyces microflavus is streptomyces actinomycetes.Containing auxin, antibiotics, phenylacetic acid, succinum in its metabolite
Growth regulator composition necessary to the plant growths such as acid and the basic element of cell division, has conversion Nitrogen In Soils, phosphorus, potassium, improves soil
Earth fertility, reduces fertilizer amount, suppresses germ reproduction, and diseases prevention is kept a full stand of seedings；Stimulate cell division, promote that crop takes root, germinates, becomes
Ripe, improve crop yield；Promoting active substance synthesis, the quality etc. significantly improving agricultural product acts on, and reaches " increasing both production and income, guarantor
Water support field, suppression disease, improve quality " quadruple effect unification effect.
Sporotrichum thermophile draws and belongs to thermophilic filamentous fungi for Moniliales, Moniliaceae, sporotrichum thermophile.There is decomposition fiber
The characteristic of element, is often known as biological active substances, can effectively improve soil, culture fertility, makes crop obtain higher yield
And fine quality.They are possible not only to regulate the ecological environment of crop root soil microorganism, increase soil beneficial microorganism
Population and quantity, and the occurrence frequency of crop root disease can also be reduced, receive the effect of fungus treatment.
Pseudomonas chlororaphis, the one of antibacterial biocontrol microorganisms, Classification system is Pseudomonas choloeaphtis.Should
It is used on Fructus Capsici, Nicotiana tabacum L., Phytophthora capsici, black shank pathogen can be prevented and treated.Physiological and biochemical property is for can produce iridescent
Element, can hydrolyze arginine dihydrolase, lipase, oxidase, catalase, citrate, gelatin, not hydrolyze starch.Thalline
For shaft-like, 0.3 μm～0.8 μ m 1.0 μm～1.1 μm, Gram-negative, without spore, single polar flagella, can move.?
Can form 1.2mm bacterium colony after cultivating 24h in KMB culture medium, bacterium colony can produce orange pigment, circular, and rat is smooth, relatively
Thickness, easily provokes, neat in edge.
Above-mentioned Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, Pseudomonas chlororaphis are permissible
The method using this area conventional is cultivated, it is possible to use commercial goods.
Detailed description of the invention
Embodiment 1
Dispensing is: Bacillus thuringiensis 6 parts, bacillus amyloliquefaciens 6 parts, streptomyces microflavus 8 parts, sporotrichum thermophile 4 parts, and green pin is false
Zymomonas mobilis 4 parts, modified zeolite powder 30 parts, potassium fulvic acid powder 4 parts, sodium alginate 3 parts, water-retaining agent 0.6 part, poly-aspartate 2 parts,
Corn starch 5 parts, tea saponin 2 parts, compound enzymic preparation 1 part, sodium lignin sulfonate 3 parts, citric acid 0.7 part, sodium sulfite 0.8
Part；
Water-retaining agent is polyacrylamide and the sodium polyacrylate of equivalent；
Compound enzymic preparation is cellulase, amylase and lignoenzyme, and the weight ratio of three is 3:1:1；
The preparation process of described modified zeolite powder includes: natural zeolite powder adds stirring 5h in concentrated nitric acid solution, filters, dry
Dry；Then zeolite uses syllogic gradient calcine, and concrete calcination operation is: (a) carries out calcining 45min at 500 DEG C；(b)
It is warmed up to 800 DEG C with the heating rate of 10 DEG C/min, and insulation calcining 45min at such a temperature；C () is with the intensification of 6 DEG C/min
Ramp to 1200 DEG C, and insulation calcining 45min at such a temperature, after calcining terminates, naturally cool to room temperature；Zeolite is put
In the ammonium nitrate solution of 1mol/L, being heated to 90 DEG C, continuously stirred reaction 6h, reaction is filtered after terminating, is washed, is dried, enters
Row micronizing, obtains modified zeolite powder.
Preparation method comprises the steps:
(1) each component raw material is weighed by described weight standby；
(2) first by load weighted Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, green pin vacation unit cell
Send into after bacterium, poly-aspartate, tea saponin, citric acid, sodium sulfite mix homogeneously in granulating disc or rotary drum granulator,
And coordinate and add appropriate steam pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by modified zeolite powder, potassium fulvic acid powder, sodium alginate, water-retaining agent, compound enzymic preparation, sodium lignin sulfonate at batch mixing
In machine after mixing, add granule and the corn starch of preparation in step (2), send into granulating disc after mix homogeneously or rotary drum is made
In grain machine, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, obtains fermenting agent；
(4) by fermenting agent 13 parts, bagasse 100 parts, Pericarppium arachidis hypogaeae 40 parts, bean cake 40 parts, chicken manure 40 parts and 40 parts of water at room temperature
Under carry out compost fermentation, fermentation time 12 days, drying and crushing obtains disease-resistant organic fertilizer；By described organic fertilizer application in soil it is
Can.
Embodiment 2
Dispensing is: Bacillus thuringiensis 5 parts, bacillus amyloliquefaciens 5 parts, streptomyces microflavus 5 parts, sporotrichum thermophile 3 parts, and green pin is false
Zymomonas mobilis 3 parts, modified zeolite powder 20 parts, potassium fulvic acid powder 1.5 parts, sodium alginate 0.5 part, water-retaining agent 0.5 part, poly-aspartate
1 part, corn starch 3 parts, tea saponin 0.5 part, compound enzymic preparation 0.5 part, sodium lignin sulfonate 1 part, citric acid 0.5 part, sulfurous
0.5 part of hydrogen sodium of acid；
Water-retaining agent is polyacrylamide and the sodium polyacrylate of equivalent；
Compound enzymic preparation is cellulase, amylase and lignoenzyme, and the weight ratio of three is 3:1:1；
The preparation process of described modified zeolite powder includes: natural zeolite powder adds stirring 3h in concentrated nitric acid solution, filters, dry
Dry；Then zeolite uses syllogic gradient calcine, and concrete calcination operation is: (a) carries out calcining 60min at 500 DEG C；(b)
It is warmed up to 800 DEG C with the heating rate of 12 DEG C/min, and insulation calcining 60min at such a temperature；C () is with the intensification of 8 DEG C/min
Ramp to 1200 DEG C, and insulation calcining 60min at such a temperature, after calcining terminates, naturally cool to room temperature；Zeolite is put
In the ammonium nitrate solution of 1mol/L, being heated to 100 DEG C, continuously stirred reaction 8h, reaction is filtered after terminating, is washed, is dried, enters
Row micronizing, obtains modified zeolite powder.
Preparation method comprises the steps:
(1) each component raw material is weighed by described weight standby；
(2) first by load weighted Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, green pin vacation unit cell
Send into after bacterium, poly-aspartate, tea saponin, citric acid, sodium sulfite mix homogeneously in granulating disc or rotary drum granulator,
And coordinate and add appropriate steam pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by modified zeolite powder, potassium fulvic acid powder, sodium alginate, water-retaining agent, compound enzymic preparation, sodium lignin sulfonate at batch mixing
In machine after mixing, add granule and the corn starch of preparation in step (2), send into granulating disc after mix homogeneously or rotary drum is made
In grain machine, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, obtains fermenting agent；
(4) by fermenting agent 10 parts, bagasse 80 parts, Pericarppium arachidis hypogaeae 30 parts, bean cake 30 parts, chicken manure 30 parts and 30 parts of water at normal temperatures
Carrying out compost fermentation, fermentation time 10 days, drying and crushing obtains disease-resistant organic fertilizer；By described organic fertilizer application in soil it is
Can.
Embodiment 3
Dispensing is: Bacillus thuringiensis 10 parts, bacillus amyloliquefaciens 10 parts, streptomyces microflavus 10 parts, and sporotrichum thermophile 5 parts is green
Pin pseudomonas 5 parts, modified zeolite powder 40 parts, potassium fulvic acid powder 5 parts, sodium alginate 5 parts, water-retaining agent 1 part, poly-aspartate 3
Part, corn starch 6 parts, tea saponin 3 parts, compound enzymic preparation 2 parts, sodium lignin sulfonate 5 parts, citric acid 1 part, sodium sulfite 1
Part；
Water-retaining agent is polyacrylamide and the sodium polyacrylate of equivalent；
Compound enzymic preparation is cellulase, amylase and lignoenzyme, and the weight ratio of three is 3:1:1；
The preparation process of described modified zeolite powder includes: natural zeolite powder adds stirring 3h in concentrated nitric acid solution, filters, dry
Dry；Then zeolite uses syllogic gradient calcine, and concrete calcination operation is: (a) carries out calcining 30min at 500 DEG C；(b)
It is warmed up to 800 DEG C with the heating rate of 8 DEG C/min, and insulation calcining 30min at such a temperature；C () is with the intensification speed of 5 DEG C/min
Rate is warmed up to 1200 DEG C, and insulation calcining 30min at such a temperature, after calcining terminates, naturally cools to room temperature；Zeolite is placed in
In the ammonium nitrate solution of 1mol/L, being heated to 80 DEG C, continuously stirred reaction 5h, reaction is filtered after terminating, is washed, is dried, carries out
Micronizing, obtains modified zeolite powder.
Preparation method comprises the steps:
(1) each component raw material is weighed by described weight standby；
(2) first by load weighted Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, green pin vacation unit cell
Send into after bacterium, poly-aspartate, tea saponin, citric acid, sodium sulfite mix homogeneously in granulating disc or rotary drum granulator,
And coordinate and add appropriate steam pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by modified zeolite powder, potassium fulvic acid powder, sodium alginate, water-retaining agent, compound enzymic preparation, sodium lignin sulfonate at batch mixing
In machine after mixing, add granule and the corn starch of preparation in step (2), send into granulating disc after mix homogeneously or rotary drum is made
In grain machine, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, obtains fermenting agent；
(4) by fermenting agent 15 parts, bagasse 120 parts, Pericarppium arachidis hypogaeae 50 parts, bean cake 50 parts, chicken manure 50 parts and 50 parts of water at room temperature
Under carry out compost fermentation, fermentation time 15 days, drying and crushing obtains disease-resistant organic fertilizer；By described organic fertilizer application in soil it is
Can.
Comparative example 1(omits poly-aspartate)
Dispensing is: Bacillus thuringiensis 6 parts, bacillus amyloliquefaciens 6 parts, streptomyces microflavus 8 parts, sporotrichum thermophile 4 parts, and green pin is false
Zymomonas mobilis 4 parts, modified zeolite powder 30 parts, potassium fulvic acid powder 4 parts, sodium alginate 3 parts, water-retaining agent 0.6 part, corn starch 5 parts, tea
Saponin 2 parts, compound enzymic preparation 1 part, sodium lignin sulfonate 3 parts, citric acid 0.7 part, sodium sulfite 0.8 part；Other are with implementing
Example 1.
Comparative example 2 (omission sodium lignin sulfonate)
Dispensing is: Bacillus thuringiensis 6 parts, bacillus amyloliquefaciens 6 parts, streptomyces microflavus 8 parts, sporotrichum thermophile 4 parts, and green pin is false
Zymomonas mobilis 4 parts, modified zeolite powder 30 parts, potassium fulvic acid powder 4 parts, sodium alginate 3 parts, water-retaining agent 0.6 part, poly-aspartate 2 parts,
Corn starch 5 parts, tea saponin 2 parts, compound enzymic preparation 1 part, citric acid 0.7 part, sodium sulfite 0.8 part；Other same embodiments
1。
Comparative example 3(omits the step using modification of ammonium nitrate zeolite)
The preparation process of described modified zeolite powder includes: natural zeolite powder adds stirring 5h in concentrated nitric acid solution, filters, dry
Dry；Then zeolite uses syllogic gradient calcine, and concrete calcination operation is: (a) carries out calcining 45min at 500 DEG C；(b)
It is warmed up to 800 DEG C with the heating rate of 10 DEG C/min, and insulation calcining 45min at such a temperature；C () is with the intensification of 6 DEG C/min
Ramp to 1200 DEG C, and insulation calcining 45min at such a temperature, after calcining terminates, naturally cool to room temperature；Carry out ultra micro
Pulverize, obtain modified zeolite powder；
Other are with embodiment 1.
Test example 1 bacteriostatic test
Use steel ring method carry out bacteriostatic test, will activation after Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, addicted to
Hot side spore is mould, Pseudomonas chlororaphis and five composite bacteria agent capables carry out bacteriostatic test, three, each sample respectively with cucumber fusarium axysporum
Repeat.Each bacterial strain is cultivated in corresponding culture medium 2-3 days (190r/min, 28 DEG C) and makes fermentation liquid, by cultured Fructus Cucumidis sativi
It is 10 that wilt makes spore concentration6The bacteria suspension of individual/mL.Take cucumber fusarium axysporum bacteria suspension 0.1mL and be applied to PDA plate
On, on the solid medium of each flat board, then put the steel ring of two prior sterilizings, then add in each steel ring
Above-mentioned each fermented liquid of 0.25mL and composite bacteria liquid, each process in triplicate, only to connect cucumber fusarium axysporum bacteria suspension
Plate is comparison.Cultivate and the antibacterial situation of routine observation for 28 DEG C.The results are shown in Table 1, "+" represent that Antagonistic reaction is positive, "+" get over multilist
Show that inhibition is the biggest.
Table 1
Result of the test in table 1 shows, Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, green pin
Pseudomonas and composite bacteria agent capable all have fungistatic effect to cucumber fusarium axysporum, and wherein composite bacteria agent capable effect is the strongest.
Test example 2 Fructus Cucumidis sativi field test
Embodiment of the present invention 1-3 is used respectively and fertilizer prepared by comparative example 1-3 in the experimental plot of unit are,
15kg/ mu, then per unit transplants cucumber seedlings 50 strain, observes diseased plant number, to check disease-resistant organic fertilizer fermenting agent of the present invention
Protection effect, experimental result is as shown in table 2.
Table 2
The result of table 2 shows, the product of embodiment of the present invention 1-3 has an obvious protection effect to cucumber seedlings, and comparative example
1-3 can make preventive effect due to the step eliminating poly-aspartate, sodium lignin sulfonate or omission modification of ammonium nitrate zeolite
Decline, illustrate that each component is the most collaborative.
Test example 3 Chinese cabbage field experiment
Embodiment of the present invention 1-3 is used respectively and fertilizer conduct prepared by comparative example 1-3 in the experimental plot of unit are
Base fertilizer 15kg/ mu, matched group is used the commercially available fertilizer of equivalent, is planted Chinese cabbage, and record yield；Each group, except the executed end
Fertile different, manuring late and management are the most identical.
Table 3
| Comparison | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Average product (kg/ mu) | 4210 | 5265 | 5300 | 5280 | 4430 | 4550 | 4500 |
The data of table 3 show, product of the present invention can substantially increase the per mu yield of Chinese cabbage.
Claims (7)
1. a disease-resistant organic fertilizer fermenting agent, is made up of the component of following weight portion: Bacillus thuringiensis 5-10 part, solves starch bud
Spore bacillus 5-10 part, streptomyces microflavus 5-10 part, sporotrichum thermophile 3-5 part, Pseudomonas chlororaphis 3-5 part, modified zeolite powder 20-
40 parts, potassium fulvic acid powder 1.5-5 part, sodium alginate 0.5-5 part, water-retaining agent 0.5-1 part, poly-aspartate 1-3 part, corn starch
3-6 part, tea saponin 0.5-3 part, compound enzymic preparation 0.5-2 part, sodium lignin sulfonate 1-5 part, citric acid 0.5-1 part, sulfurous acid
Hydrogen sodium 0.5-1 part.
Disease-resistant organic fertilizer fermenting agent the most according to claim 1, described water-retaining agent is the polyacrylamide of equivalent and gathers
Sodium acrylate.
Disease-resistant organic fertilizer fermenting agent the most according to claim 1, described compound enzymic preparation be cellulase, amylase and
Lignoenzyme, the weight ratio of three is 3:1:1.
Disease-resistant organic fertilizer fermenting agent the most according to claim 1, the preparation process bag of wherein said modified zeolite powder
Include: natural zeolite powder is added stirring 3-6h in concentrated nitric acid solution, filters, be dried；Then zeolite use syllogic gradient forge
Burning, concrete calcination operation is: (a) carries out calcining 30-60min at 500 DEG C；B () is heated up with the heating rate of 8-12 DEG C/min
To 800 DEG C, and insulation calcining 30-60min at such a temperature；C () is warmed up to 1200 DEG C with the heating rate of 5-8 DEG C/min, and
Insulation calcining 30-60min, after calcining terminates, naturally cools to room temperature at such a temperature；Zeolite is placed in the ammonium nitrate of 1mol/L
In solution, it is heated to 80-100 DEG C, continuously stirred reaction 5-8h, reaction is filtered after terminating, is washed, dry, carries out micronizing,
Obtain modified zeolite powder.
Disease-resistant organic fertilizer fermenting agent the most according to claim 1, is prepared into grain shape, or general further
Grain loads in capsule.
6. according to the preparation method of a kind of disease-resistant organic fertilizer fermenting agent described in any one of claim 1-5, it is characterised in that
Comprise the steps:
(1) each component raw material is weighed by described weight standby；
(2) first by load weighted Bacillus thuringiensis, bacillus amyloliquefaciens, streptomyces microflavus, sporotrichum thermophile, green pin vacation unit cell
Send into after bacterium, poly-aspartate, tea saponin, citric acid, sodium sulfite mix homogeneously in granulating disc or rotary drum granulator,
And coordinate and add appropriate steam pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by modified zeolite powder, potassium fulvic acid powder, sodium alginate, water-retaining agent, compound enzymic preparation, sodium lignin sulfonate at batch mixing
In machine after mixing, add granule and the corn starch of preparation in step (2), send into granulating disc after mix homogeneously or rotary drum is made
In grain machine, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, to obtain final product.
The using method of disease-resistant organic fertilizer fermenting agent the most according to claim 1, including by fermenting agent 10-15 part,
Bagasse 80-120 part, Pericarppium arachidis hypogaeae 30-50 part, bean cake 30-50 part, chicken manure 30-50 part and water 30-50 part carry out heap at normal temperatures
Fertile fermentation, fermentation time 10-15 days, drying and crushing obtains disease-resistant organic fertilizer, and part is weight portion；By described organic fertilizer application in soil
In earth.
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