CN103898019A - Continuous cropping resistant composite microbial agent and preparation method thereof - Google Patents
Continuous cropping resistant composite microbial agent and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of biological fertilizers, and in particular relates to a continuous cropping resistant composite microbial agent and a preparation method thereof. The continuous cropping resistant composite microbial agent is prepared from the following raw materials in percentage by weight: 20-60% of composite microbial agent, 2-10% of trace elements, 20-70% of calcium carbonate and 5-10% of drying agent. The preparation method for the continuous cropping resistant composite microbial agent comprises the following steps: (1) culture of bacterial liquid of various bacilli; (2) preparation of the composite microbial agent; (3) preparation of the continuous cropping resistant composite microbial agent. The production process provided by the invention is refined; the product provided by the invention can promote quick growth of crops, adjust the vital movement of the crops, increase both production and income, obviously improve the quality of fruits, and decompose organic matters and toxic and harmful matters so as to form a protective barrier in the root environment of the crops and enhance the stress resistance of the crops.
Description
Technical field
The invention belongs to bio-feritlizer technical field, relate in particular to a kind of anti-continuous cropping complex micro organism fungicide and preparation method thereof.
Background technology
In recent years, increasingly serious along with global Desertification, desertification situation, can plough soil fewer and feweri, and the contradiction that China has a large population and a few land is more outstanding, and raise the multiple-cropping index (continuous cropping farming) is unique way of dealing with problems.But the continuous cropping diseases such as consequent soil-borne disease cause crop to drop in production over a large area, have no harvest.General sickness rate is at 10-30%, and plant is usually withered, causes the disconnected ridge that is short of seedling, and seriously can reach 80-90%, and even full garden death, causes total crop failure, and continuous cropping disease is a kind of destructive disease.Continuous cropping disease becomes the emphasis problem of agricultural experts and vast farmers research and concern, but never has for a long time great breakthrough.
Under the envrionment conditions of continuous cropping, in soil, there is germ insect, also have these " dirty " things of toxic substance to exist, the pathogenic micro-organisms such as fungus and bacterium are through amount reproduction, the constantly root system of infringement plant, cause crop to be subject to the harm such as blight, leaf blight, virus disease, had a strong impact on plant growth.
Continuous cropping is all very large to the harm of vegetables and fruit, continuous cropping continuous cropping makes many crops as bad in plant growths such as leguminous plants, melon, vegetables, strawberry and some herbal medicine, as peach kind 4 years, always childlike, stature, quality are also bad, the chief reason development and growth of root system that has been exactly the bacteria effect that hands down by heredity of continuous cropping, so peach can not grown.In same piece of land, plant continuously same crop, can bring so serious consequence to be because, plant is in process of growth, secrete some objectionable impuritiess, these objectionable impuritiess accumulate in the middle of soil, 1 year accumulation part, if the words of planting continuously, within several years, get off, it is many that objectionable impurities just accumulates, and in cultivating continuously, the root system of crop infringement is just more serious like this.What occur now macerates root, dead root, and also having root-rot phenomenon is exactly more seriously the impact of this respect.But, germ is also selective to the harm of farm crop, such as the germ of harm cucumber might not be harmful to peanut, if but on a plot, plant continuously cucumber, disease will accumulate continuously, is exactly the soil-borne disease that common people often say.The method of avoiding the common use of continuous cropping is the rotation of crops, and kind cucumber, will just plant capsicum next year in this year, now for the ease of unified management and sale, all advocates the single kind of regional plantation, and like this, the problem of continuous cropping is just difficult to solve.
Microbiobacterial agent is the one of microbial fertilizer, and production cost is low, and effect is good, and therefore its effect in agricultural is familiar with by people gradually.Microbiobacterial agent is not only applicable to growing vegetables, and the disease that solves fruit tree is also produced effect.Vegetables can also rotate crops, and are exactly within 10 years 8 years, not move place after under fruit tree kind, and it is more that harmful germ accumulates, and more needs to eliminate disease.But existing microbiobacterial agent effect in anti-continuous cropping process is not very desirable.
Summary of the invention
First technical problem to be solved by this invention is: the deficiency existing for prior art, and provide one can improve crop to Nutrient Absorption rate, decompose hazardous and noxious substances, promote the anti-continuous cropping complex micro organism fungicide of plant root growth.
Second technical problem to be solved by this invention is: the deficiency existing for prior art, and provide one can improve crop to Nutrient Absorption rate, decompose hazardous and noxious substances, promote the preparation method of the anti-continuous cropping complex micro organism fungicide of plant root growth.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
A kind of anti-continuous cropping complex micro organism fungicide is to be prepared from by the raw material that comprises following weight percent: the calcium carbonate of the complex micro organism fungicide of 20～60wt%, the trace element of 2～10wt%, 20～70wt%, the siccative of 5～10wt%.
Preferably, described complex micro organism fungicide is two or more the compound in subtilis, Bacillus licheniformis, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus and bacillus amyloliquefaciens.
Preferably, described trace element is one or more the composition in chelated copper, chelated iron, chelated zinc, Chelated Manganese, boric acid and ammonium molybdate.
Preferably, described siccative is the one in wilkinite, white carbon black and anti-hard caking agent.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
A preparation method for anti-continuous cropping complex micro organism fungicide, comprises the following steps:
(1) cultivation of various genus bacillus bacterium liquid: be 20 ℃～35 ℃ in temperature, under air tight condition, by the bacterial classification of subtilis, Bacillus licheniformis, bacillus megaterium, colloid bacillus cereus, bacillus laterosporus and bacillus amyloliquefaciens inoculation, activation culture 10～36 hours in activation medium respectively, then cultured bacterial classification is fermented and expanded numerous 24～72 hours respectively in production fermention medium, in described activation culture and the numerous process of fermentation expansion, constantly shake or stir, obtaining the bacterium liquid of above-mentioned various genus bacillus.
(2) preparation of complex micro organism fungicide: by the bacterium liquid of the various genus bacillus of step (1) gained respectively through diatomite support or light calcium carbonate carrier adsorption, oven dry, pulverizing, obtain the bacterium powder of various genus bacillus, get the bacterium powder of two or more above-mentioned genus bacillus, mix, obtain complex micro organism fungicide.
(3) preparation of anti-continuous cropping complex micro organism fungicide: the complex micro organism fungicide of step (2) gained is mixed with the calcium carbonate crushing, siccative, trace element, obtain anti-continuous cropping complex micro organism fungicide.
Wherein, the quality percentage composition of described complex micro organism fungicide and each raw material is: complex micro organism fungicide 20～60wt%; Trace element 2～10wt%; Calcium carbonate 20～70wt%; Siccative 5～10wt%.
Wherein, described trace element is one or more the composition in chelated copper, chelated iron, chelated zinc, Chelated Manganese, boric acid and ammonium molybdate; Described siccative is the one in wilkinite, white carbon black, anti-hard caking agent.
Wherein, described activation medium is that mass percent is peptone, beef leaching thing, sodium-chlor, agar and the sterilized water of 10:3:5:15:1000; Described production fermention medium is that mass percent is peptone, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, potassium primary phosphate and the sterilized water of 200:10:5:400:300:100:10:4:2000; The pH of described activation medium and production fermention medium is 7.2～7.8.
Improve as one, first by described activation medium with produce fermention medium high-temperature sterilization after 30～40 minutes at 121 ℃ respectively, then add the bacterial classification of above-mentioned various genus bacillus to cultivate.
Improve as one, after in step (3), described complex micro organism fungicide being dried, pulverized, then mix with the trace element crushing, calcium carbonate, siccative, obtain anti-continuous cropping complex micro organism fungicide.
Owing to having adopted technique scheme, the invention has the beneficial effects as follows:
The present invention can promote crop Fast Growth, in the present invention, contained complex micro organism fungicide is bacillus, the bacillus such as subtilis are profitable strain, this profitable strain produces a large amount of plant endogenous enzymes in metabolic process, can obviously improve the specific absorption of crop to nutritive elements such as nitrogen phosphorus potassium, research shows that product of the present invention can improve utilization rate of nitrogen fertilizer 10～20%, phosphate fertilizer utilization efficiency 15～30%, potassium utilization rate 10～25%.
The present invention can regulate the vital movement of crop, increasing both production and income, profitable strain also can promote crop root growth, fibrous root is increased, plant endogenous enzyme and plant-growth regulator that beneficial microbe colony metabolism produces enter in plant materials via root system, promote leaf photosynthesis, regulate nutritive element to flow toward fruit, swollen fruit effect of increasing production is obvious.
The present invention can obviously improve fruit quality, and profitable strain can reduce nitrate content in plant materials, and the heavy metal that can degrade in soil can improve Vc content in fruit and the content of soluble sugar; Profitable strain also can improve the content of indispensable amino acid in fruit (Methionin and methionine(Met)), vitamin B complex and unsaturated fatty acids etc., and in the present invention, also contain trace element, can supply with plant vital activity required, its fruit mouthfeel is good, and storage endurance, selling price are high.
The present invention can decomposing organic matter matter and hazardous and noxious substances, profitable strain can disengage highly active amylase, the macromolecular substance that difficulty is decomposed resolves into available small-molecule substance, can accelerate the decomposition of organic substance, for crop is manufactured available nutrient and power is provided, suppress the growth and breeding of the harmful microbes such as harmful bacteria, pathogenic bacteria and can decompose continuous cropping hazardous and noxious substances, thus control continuous cropping.
The present invention makes the rhizospheric environment of crop form protective barrier; after profitable strain is manured into soil; breeding becomes dominant microflora rapidly; control rhizosphere nutrition and the resource of crop; make the pathogenic bacterias such as continuous cropping, root-rot, vertical withered, gummosis, grey mold lose living space and condition, make root system of plant cell wall thickening, fibrosis, lignifying; and form the two silicon layers of colloid, form the firm barrier that stops pathogenic bacteria invasion and attack.
The present invention can strengthen crop anti-adversity, and the moisture retention of profitable strain is very strong, can strengthen buffering power of soil, and water conservation insulation, strengthens crop drought resistance, cold tolerance; Also can strengthen blade protection film simultaneously, opposing pathogen infection, disease-resistant, pest-resistant.
The present invention adopts substratum that various genus bacillus are inoculated respectively, fermented, adsorb, be dried, pulverize and obtain various genus bacillus bacterium powder by press filtration through the bacterium liquid that obtains of fermentation, be mixed to get complex micro organism fungicide according to fixed ratio, again itself and trace element, calcium carbonate, siccative are mixed according to a certain percentage to the anti-continuous cropping complex micro organism fungicide making, its production technique refining, be rich in multiple beneficial bacterium, mainly for the problem such as soil poison accumulation, soil-borne disease that continuous cropping brings at present.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment mono-
The peptone that is 10:3:5:15:1000 by mass percent, beef leaching thing, sodium-chlor, the peptone of the activation medium of agar and sterilized water and mass percent 200:10:5:400:300:100:10:4:2000, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, the production fermention medium of potassium primary phosphate and sterilized water is high-temperature sterilization 30 minutes at 121 ℃ respectively, the pH of described activation medium and production fermention medium is 7.5, respectively subtilis and Bacillus licheniformis are inoculated in activation medium, 30 ℃ of activation culture 15 hours in isothermal vibration incubator, then the fermentation in production fermention medium of cultured each bacterial classification is expanded numerous 24 hours, stirred once every 2 hours during this time, make respectively subtilis bacterium liquid and bacillus licheniformis bacterium liquid, respectively by above-mentioned two kinds of bacterium liquid absorption, press filtration, dry, pulverize, make subtilis powder and Bacillus licheniformis powder.
Subtilis powder and Bacillus licheniformis powder are mixed according to the ratio of 1:1, make complex micro organism fungicide; Chelated copper, chelated zinc and ammonium molybdate are mixed according to the ratio of 2:1:2, make trace element, pulverize, for subsequent use.
Get the trace element after above-mentioned complex micro organism fungicide and pulverizing, get again the calcium carbonate and the siccative wilkinite that crush appropriate, each raw material mixes and obtains anti-continuous cropping complex micro organism fungicide, wherein the weight percent of each raw material is: complex micro organism fungicide 60wt%, trace element 5wt%, calcium carbonate 25%, wilkinite 10%.
Embodiment bis-
The peptone that is 10:3:5:15:1000 by mass percent, beef leaching thing, sodium-chlor, the peptone of the activation medium of agar and sterilized water and mass percent 200:10:5:400:300:100:10:4:2000, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, the production fermention medium of potassium primary phosphate and sterilized water is high-temperature sterilization 35 minutes at 121 ℃ respectively, the pH of described activation medium and production fermention medium is 7.2, respectively bacillus laterosporus and bacillus megaterium are inoculated in activation medium, 20 ℃ of activation culture 36 hours in isothermal vibration incubator, then the fermentation in production fermention medium of cultured each bacterial classification is expanded numerous 72 hours, stirred once every 2 hours during this time, make respectively bacillus laterosporus bacterium liquid and Bacillus megatherium bacterium liquid, respectively by above-mentioned two kinds of bacterium liquid absorption, press filtration, dry, pulverize, make bacillus laterosporus powder and bacillus megaterium powder.
Bacillus laterosporus powder and bacillus megaterium powder are mixed according to the ratio of 5:1, make complex micro organism fungicide; Chelated iron, Chelated Manganese and boric acid are mixed according to the ratio of 1:2:2, make trace element, pulverize, for subsequent use.
Get the trace element after above-mentioned complex micro organism fungicide and pulverizing, get again the calcium carbonate and the siccative white carbon black that crush appropriate, each raw material mixes and obtains anti-continuous cropping complex micro organism fungicide, wherein the weight percent of each raw material is: complex micro organism fungicide 20wt%, trace element 2wt%, calcium carbonate 70%, white carbon black 8%.
Embodiment tri-
The peptone that is 10:3:5:15:1000 by mass percent, beef leaching thing, sodium-chlor, the peptone of the activation medium of agar and sterilized water and mass percent 200:10:5:400:300:100:10:4:2000, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, the production fermention medium of potassium primary phosphate and sterilized water is high-temperature sterilization 33 minutes at 121 ℃ respectively, the pH of described activation medium and production fermention medium is 7.4, respectively colloid bacillus cereus and bacillus amyloliquefaciens are inoculated in activation medium, 28 ℃ of activation culture 30 hours in isothermal vibration incubator, then the fermentation in production fermention medium of cultured each bacterial classification is expanded numerous 65 hours, stirred once every 2 hours during this time, make respectively colloid bacillus cereus bacterium liquid and bacillus amyloliquefaciens bacterium liquid, respectively by above-mentioned two kinds of bacterium liquid absorption, press filtration, dry, pulverize, make colloid bacillus cereus powder and bacillus megaterium powder.
Colloid bacillus cereus powder and bacillus amyloliquefaciens powder are mixed according to the ratio of 5:1, make complex micro organism fungicide; Chelated copper, Chelated Manganese and chelated zinc are mixed according to the ratio of 2:1:1, make trace element, pulverize, for subsequent use.
Get the trace element after above-mentioned complex micro organism fungicide and pulverizing, get again the calcium carbonate and the siccative anti-hard caking agent that crush appropriate, each raw material mixes and obtains anti-continuous cropping complex micro organism fungicide, wherein the weight percent of each raw material is: complex micro organism fungicide 30wt%, trace element 4wt%, calcium carbonate 60%, anti-hard caking agent 6%.
Embodiment tetra-
The peptone that is 10:3:5:15:1000 by mass percent, beef leaching thing, sodium-chlor, agar, the peptone of the activation medium of sterilized water and mass percent 200:10:5:400:300:100:10:4:2000, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, the production fermention medium of potassium primary phosphate and sterilized water is high-temperature sterilization 30 minutes at 121 ℃ respectively, and described activation medium and the pH that produces fermention medium are 7.6, respectively by subtilis, Bacillus licheniformis, bacillus laterosporus, bacillus megaterium, the bacterial classification of colloid bacillus cereus and bacillus amyloliquefaciens is inoculated in activation medium, 35 ℃ of activation culture 20 hours in isothermal vibration incubator, then phase expands the fermentation in production fermention medium of cultured bacterial classification numerous 34 hours, opened every 2 hours during this time once and stir, make respectively various genus bacillus bacterium liquid, respectively bacterium liquid is adsorbed, press filtration, dry, pulverize, make the bacterium powder of various genus bacillus.
Subtilis powder, Bacillus licheniformis powder, bacillus laterosporus powder, bacillus megaterium powder, colloid bacillus cereus powder, bacillus amyloliquefaciens powder are mixed according to the ratio of 1:1:8:2:1:1, make complex micro organism fungicide; Chelated zinc, boric acid, Chelated Manganese are mixed according to the ratio of 2:1:1, make trace element, pulverize, for subsequent use.
Get the trace element after above-mentioned complex micro organism fungicide and pulverizing, get again the calcium carbonate and the siccative wilkinite that crush appropriate, each raw material mixes and obtains anti-continuous cropping complex micro organism fungicide, wherein the weight percent of each raw material is: complex micro organism fungicide 40wt%, trace element 10wt%, calcium carbonate 45%, wilkinite 5%.
Embodiment five
The peptone that is 10:3:5:15:1000 by mass percent, beef leaching thing, sodium-chlor, agar, the peptone of the activation medium of sterilized water and mass percent 200:10:5:400:300:100:10:4:2000, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, the production fermention medium of potassium primary phosphate and sterilized water is high-temperature sterilization 40 minutes at 121 ℃ respectively, and described activation medium and the pH that produces fermention medium are 7.7, respectively by subtilis, Bacillus licheniformis, bacillus laterosporus, bacillus megaterium, the bacterial classification of colloid bacillus cereus and bacillus amyloliquefaciens is inoculated in activation medium, 35 ℃ of activation culture 24 hours in isothermal vibration incubator, then phase expands the fermentation in production fermention medium of cultured bacterial classification numerous 48 hours, opened every 2 hours during this time once and stir, make respectively various genus bacillus bacterium liquid, respectively bacterium liquid is adsorbed, press filtration, dry, pulverize, make the bacterium powder of various genus bacillus.
Subtilis powder, Bacillus licheniformis powder, bacillus laterosporus powder, bacillus megaterium powder, colloid bacillus cereus powder, bacillus amyloliquefaciens powder are mixed according to the ratio of 1:2:9:2:2:1, make complex micro organism fungicide; Just chelated zinc, chelated iron and Chelated Manganese mix according to the ratio of 2:2:1, make trace element, pulverize, for subsequent use.
Get the trace element after above-mentioned complex micro organism fungicide and pulverizing, get again the calcium carbonate and the siccative white carbon black that crush appropriate, each raw material mixes and obtains anti-continuous cropping complex micro organism fungicide, wherein the weight percent of each raw material is: complex micro organism fungicide 35wt%, trace element 5wt%, calcium carbonate 55%, white carbon black 5%.
Embodiment six
The peptone that is 10:3:5:15:1000 by mass percent, beef leaching thing, sodium-chlor, agar, the peptone of the activation medium of sterilized water and mass percent 200:10:5:400:300:100:10:4:2000, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, the production fermention medium of potassium primary phosphate and sterilized water is high-temperature sterilization 35 minutes at 121 ℃ respectively, and described activation medium and the pH that produces fermention medium are 7.8, respectively by subtilis, Bacillus licheniformis, bacillus laterosporus, bacillus megaterium, the bacterial classification of colloid bacillus cereus and bacillus amyloliquefaciens is inoculated in activation medium, 35 ℃ of activation culture 32 hours in isothermal vibration incubator, then phase expands the fermentation in production fermention medium of cultured bacterial classification numerous 50 hours, opened every 2 hours during this time once and stir, make respectively various genus bacillus bacterium liquid, respectively bacterium liquid is adsorbed, press filtration, dry, pulverize, make the bacterium powder of various genus bacillus.
Subtilis powder, Bacillus licheniformis powder, bacillus laterosporus powder, bacillus megaterium powder, colloid bacillus cereus powder, bacillus amyloliquefaciens powder are mixed according to the ratio of 2:1:7:1:2:1, make complex micro organism fungicide; Just chelated zinc, chelated iron, chelated copper mix according to the ratio of 1:2:2, make trace element, pulverize, for subsequent use.
Get the trace element after above-mentioned complex micro organism fungicide and pulverizing, get again the calcium carbonate and the siccative anti-hard caking agent that crush appropriate, each raw material mixes and obtains anti-continuous cropping complex micro organism fungicide, wherein the weight percent of each raw material is: complex micro organism fungicide 30wt%, trace element 5wt%, calcium carbonate 60%, anti-hard caking agent 5%.
Test one (cucumber experiment in cultivation in plastic greenhouse)
Test is divided into control group district, test group one district and test group 2nd district, and every district area is 40 ㎡, 240 strains of every district's plantation cucumber.Control group is executed common chemical fertilizer (mainly nitrogenous, phosphorus, three kinds of elements of potassium) (3kg/ ㎡), test group one district executes the anti-continuous cropping complex micro organism fungicide (3kg/ ㎡) in above-described embodiment two, and test group 2nd district execute the anti-continuous cropping complex micro organism fungicide (3kg/ ㎡) in above-described embodiment four.Plant continuously cucumber 6 years for the plastic greenhouse of testing, soil fertility is medium.
Cucumber yield, the fruit quality results and analysis in control group district, test group one district and test group 2nd district:
| ? | Output (kg) | Soluble sugar (%) | Nitrate (mg/kg) | Vc（mg/kg） |
| Control group district | 202 | 1.8 | 68 | 73 |
| Test group one district | 243 | 2.0 | 55 | 95 |
| Test group 2nd district | 264 | 2.2 | 51 | 98 |
As can be seen from the above table, compared with using common chemical fertilizer, after using the present invention, cucumber can be increased production more than 20%, the soluble sugar ratio containing in cucumber used common chemical fertilizer height 2～4 degree, Vc content ratio has been used common chemical fertilizer height more than 30%, nitrate content ratio is used common chemical fertilizer and has been reduced more than 20%, and cucumber fruits quality is obviously improved.
Test two (field corn planting experiments)
Test is divided into control group district, test group one district and test group 2nd district, and every district area is 30 ㎡, maize planting 180 strains of every district.Control group is executed common chemical fertilizer (mainly nitrogenous, phosphorus, three kinds of elements of potassium) (3kg/ ㎡), test group one district executes the anti-continuous cropping complex micro organism fungicide (3kg/ ㎡) in above-described embodiment three, and test group 2nd district execute the anti-continuous cropping complex micro organism fungicide (3kg/ ㎡) in above-described embodiment six.For the continuous maize planting in farmland tested 8 years, soil fertility was medium.
Results and analysis:
| ? | Output (kg) |
| Control group district | 24 |
| Test group one district | 30 |
| Test group 2nd district | 32 |
As can be seen from the above table, compared with using common chemical fertilizer, use the present invention after corn can increase production more than 25%, its output has obviously improved.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. an anti-continuous cropping complex micro organism fungicide, it is characterized in that being prepared from by the raw material that comprises following weight percent: the calcium carbonate of the complex micro organism fungicide of 20～60wt%, the trace element of 2～10wt%, 20～70wt%, the siccative of 5～10wt%.
2. anti-continuous cropping complex micro organism fungicide as claimed in claim 1, is characterized in that: described complex micro organism fungicide is two or more the compound in subtilis, Bacillus licheniformis, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus and bacillus amyloliquefaciens.
3. anti-continuous cropping complex micro organism fungicide as claimed in claim 1, is characterized in that: described trace element is one or more the composition in chelated copper, chelated iron, chelated zinc, Chelated Manganese, boric acid and ammonium molybdate.
4. anti-continuous cropping complex micro organism fungicide as claimed in claim 1, is characterized in that: described siccative is the one in wilkinite, white carbon black and anti-hard caking agent.
5. a preparation method for anti-continuous cropping complex micro organism fungicide, comprises the following steps:
(1) cultivation of various genus bacillus bacterium liquid: be 20 ℃～35 ℃ in temperature; under air tight condition; by the bacterial classification of subtilis, Bacillus licheniformis, bacillus megaterium, colloid bacillus cereus, bacillus laterosporus and bacillus amyloliquefaciens inoculation, activation culture 10～36 hours in activation medium respectively; then cultured bacterial classification is fermented and expanded numerous 24～72 hours respectively in production fermention medium; in described activation culture and the numerous process of fermentation expansion, constantly shake or stir, obtaining the bacterium liquid of above-mentioned various genus bacillus;
(2) preparation of complex micro organism fungicide: by the bacterium liquid of the various genus bacillus of step (1) gained respectively through diatomite support or light calcium carbonate carrier adsorption, oven dry, pulverizing; obtain the bacterium powder of various genus bacillus; get the bacterium powder of two or more above-mentioned genus bacillus; mix, obtain complex micro organism fungicide;
(3) preparation of anti-continuous cropping complex micro organism fungicide: the complex micro organism fungicide of step (2) gained is mixed with the calcium carbonate crushing, siccative, trace element, obtain anti-continuous cropping complex micro organism fungicide.
6. the preparation method of anti-continuous cropping complex micro organism fungicide as claimed in claim 5, is characterized in that the quality percentage composition of described complex micro organism fungicide and each raw material is: complex micro organism fungicide 20～60wt%; Trace element 2～10wt%; Calcium carbonate 20～70wt%; Siccative 5～10wt%.
7. the preparation method of anti-continuous cropping complex micro organism fungicide as claimed in claim 6, is characterized in that: described trace element is one or more the composition in chelated copper, chelated iron, chelated zinc, Chelated Manganese, boric acid and ammonium molybdate; Described siccative is the one in wilkinite, white carbon black, anti-hard caking agent.
8. the preparation method of anti-continuous cropping complex micro organism fungicide as claimed in claim 5, is characterized in that: described activation medium is that mass percent is peptone, beef leaching thing, sodium-chlor, agar and the sterilized water of 10:3:5:15:1000; Described production fermention medium is that mass percent is peptone, extractum carnis, magnesium chloride, Semen Maydis powder, sucrose, starch, ferrous sulfate, potassium primary phosphate and the sterilized water of 200:10:5:400:300:100:10:4:2000; The pH of described activation medium and production fermention medium is 7.2～7.8.
9. the preparation method of anti-continuous cropping complex micro organism fungicide as claimed in claim 5, it is characterized in that: first by described activation medium with produce fermention medium high-temperature sterilization after 30～40 minutes at 121 ℃ respectively, then add the bacterial classification of above-mentioned various genus bacillus to cultivate.
10. the preparation method of anti-continuous cropping complex micro organism fungicide as claimed in claim 5, it is characterized in that: after in step (3), described complex micro organism fungicide being dried, pulverized, mix with the trace element crushing, calcium carbonate, siccative again, obtain anti-continuous cropping complex micro organism fungicide.
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| CN104478616A (en) * | 2014-12-29 | 2015-04-01 | 安徽飞天农用生物科技股份有限公司 | High-content inorganic biological bacterial fertilizer and preparation method thereof |
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