CN105601449A - Special continuous cropping resisting complexing agent for cucumbers - Google Patents
Special continuous cropping resisting complexing agent for cucumbers Download PDFInfo
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- CN105601449A CN105601449A CN201511018219.5A CN201511018219A CN105601449A CN 105601449 A CN105601449 A CN 105601449A CN 201511018219 A CN201511018219 A CN 201511018219A CN 105601449 A CN105601449 A CN 105601449A
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- complexing agent
- continuous cropping
- solid fungicide
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a special continuous cropping resisting complexing agent for cucumbers. The complexing agent is prepared by mixing a bacillus subtilis solid inoculant, a bacillus amyloliquefaciens solid inoculant, a streptomyces microflavus solid inoculant, a phosphor and potassium compound fertilizer serving as a biobacterial manure and a trace element additive according to the mass ratio of (18 to 25): (8 to 12): (8 to 12): (50 to 65): (0.8 to 1.2). According to the special continuous cropping resisting complexing agent disclosed by the invention, the defects of the prior art are overcome, a microbial ecological environment of the soil is improved, nutritive elements such as phosphor and potassium are provided for the growth of the cucumbers, the root system growth of crops is promoted, diseases of the cucumbers are reduced, and the yield increase of the cucumbers is effectively realized.
Description
Technical field
The present invention relates to microorganism complex preparation technical field, refer in particular to a kind of anti-continuous cropping of cucumber that is specifically designed toComplexing agent.
Background technology
In recent years, industrialized agriculture in China's development rapidly. The vegetables of many higher economic worths, as cucumber existsIn facility plastic greenhouse, start a large amount of plantations. Continuous cropping is also named continuous cropping, refers on a piece of land plantation continuously samePlant crop. Many crops are as melon, vegetables, strawberry and some Chinese herbal medicine etc., all because continuous cropping causes root of the cropPortion germ, causes crop generation disease, affects plant growth, thereby causes crop failure, can make when seriousBecome total crop failure. The main cause that causes continuous cropping disease to occur is due to plantation year after year, the pathogenic microorganism in soilAccumulation, progressively increases the weight of soil-borne disease insect pest year by year; Because grow the same crop for many years, cause in soil simultaneouslyThe nutrient deficiency of crop institute preference or unbalance; And the secretion of preceding crop root system and residue are dividingThe noxious material producing in solution process also impacts succession crop.
Along with the fast development of green cucumber, continuous cropping continuous cropping problem more and more becomes the focus that plantation family is paid close attention to,Many fields are because continuous cropping causes the underproduction, even total crop failure, and the physiological disturbance of cucumber also increases the weight of year by year, and this is notOnly cause the decline of cucumber yield, quality, also the income at impact plantation family.
Cucumber is wanted the prevention and control of the many employings of continuous cropping plantation medicine at present, but because traditional planting measure is prevented and treated methodCan not fundamentally control the generation of disease, prevention effect is very micro-; And use for a long time chemicals can make soilIn pathogen develop immunity to drugs, simultaneously excessive agricultural chemicals uses also micro-ecological environment that can spoiled soil, addsThe pollution of heavy environment, causes harmful and noxious substance doing residual in object. Some area adopts the side of graftingThe problem of formula prevention and control cucumber continuous cropping plantation. Although being that the one of solution continuous cropping obstacle is reasonable, grafting effectively arrangesExecute, but time-consuming cost is higher.
Along with the development of green planting, microbial manure starts extensive use in agricultural modernization plantation.Microbial manure improving Ecological Distribution of Soil Microorganisms structure, promote crop root growth, improve chemical fertilizer utilization,There is huge work crop occurs, improves the disease that reduces crops quality, the aspect that ensures food safetyWith.
Be badly in need of developing a kind of complex micro organism fungicide that is specifically designed to the anti-continuous cropping of cucumber based on foregoing.
Summary of the invention
The object of this invention is to provide the special anti-continuous cropping complexing agent of a kind of cucumber, this complexing agent can be by improvingThe microbial ecosystem of soil suppresses the breeding of soil germ, virus, the generation of containment disease, simultaneously energyDecompose the harmful substance in soil, secretion polysaccharide, promotes crop root growth.
For achieving the above object, the technical solution used in the present invention is: the special anti-continuous cropping complexing agent of a kind of cucumber,Described complexing agent is by bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide, thin yellow chainMould solid fungicide, microbial-bacterial fertilizer phosphorus potassium complex fertilizer and trace mineral supplement are according to mass ratio 18～25:The ratio mixture of 8～12:8～12:50～65:0.8～1.2 is made.
Preferably, described bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide and carefully yellowStreptomycete solid fungicide is prepared from by following processing step:
The preparation of A, liquid seeds liquid
A1, by the bacillus subtilis, bacillus amyloliquefaciens that preserve and streptomyces microflavus original strain respectivelyWith after plate streak activation, in the fluid nutrient medium of picking list colony inoculation after sterilizing, respectively through test tube,Triangular flask two-stage expands to cultivate makes liquid strain daughter bacteria liquid, and condition of culture is rotating speed 200～250r/min, cultivation28～30 DEG C of temperature, cultivation cycle 24h;
Fluid nutrient medium used is beef-protein medium, is the raw material by following mass percentMake: beef extract 0.45～0.55%, peptone 0.95～1.05%, sodium chloride 0.25～0.3%, surplus isWater, the pH value of described culture medium is controlled at 7.2～7.6;
A2, the koji tray culture medium that the liquid strain daughter bacteria liquid of preparation in steps A 1 is inoculated into after sterilizing are cultivated,The inoculum concentration of liquid strain daughter bacteria liquid be after sterilizing koji tray culture medium quality 3～5%, condition of culture is temperature28～30 DEG C, humidity 50%～70%, cultivation cycle 5～7 days, koji tray is cultivated sampling before discharging, with dull and stereotyped meterNumber method is measured spore content, and spore content is not less than 4,000,000,000/gram;
Wherein, the culture medium that koji tray is cultivated is made up of the dry raw material of following mass percent:
Wheat bran 75～80%, starch 10～15%, corn flour 3～5%, soya-bean cake 3～4%, potassium nitrate 0.09～0.1%,Dipotassium hydrogen phosphate 0.04～0.05%, magnesium sulfate 0.04～0.05%, sodium chloride 0.02～0.03%, koji tray is cultivatedIn the preparation, material-water ratio is 1:1.1 to culture medium :～1.2;
A3, after steps A 2 dischargings, stir koji tray and smashed, under 40～45 DEG C of conditions, be dried toBe 10～15% containing water quality percentage composition, after pulverizing, make respectively bacillus subtilis solid fungicide, separate starchBacillus solid fungicide, streptomyces microflavus solid fungicide.
Processing step and technical parameter concrete in the present invention are:
In described steps A 1, the sterilising conditions of liquid seed culture medium is that temperature is 121 DEG C, and the time is30min。
In described steps A 2, the sterilising conditions of koji tray culture medium is that temperature is 121 DEG C, and the time is 30min.
Preferably, described microbial-bacterial fertilizer phosphorus potassium complex fertilizer is selected and is carried out GB20287-2006 standard, micro-200,000,000/gram above biological potassium phosphorus or phosphorus potassium micro organism composite fertilizers of biologically effective bacterial content.
Further preferably, described microbial-bacterial fertilizer phosphorus potassium complex fertilizer is selected from Bei Jinghui and closes biological husbantry high-tech" favour is closed " board microbial bacterial agent, Shi Nong bio tech ltd, Xiamen that skill Co., Ltd produces produce" bacterium obtains rich " board that " good Tian Da " board microbial manure, Jun De bio tech ltd, Shandong produce is micro-One in biological compound fertilizer.
Preferably, in described trace mineral supplement, the content of manganese, zinc, boron, the each element of molybdenum is all not lowIn 3% of described trace mineral supplement gross mass.
Here, the trace element in described trace mineral supplement can select multiple water-soluble rear boron, zinc,Molybdenum, manganese element, with the compound of ionic forms, do not depart from essence of the present invention. Wherein comparatively preferred skillArt scheme is, in described trace mineral supplement, manganese element is selected from manganese sulfate, zinc element be selected from zinc sulfate orOne in zinc chloride, boron element is selected from the one in borax or boric acid, and molybdenum element is selected from ammonium molybdate.
Further preferably, borax or boric acid, zinc sulfate or zinc chloride, molybdenum in described trace mineral supplementThe ratio preparation that acid ammonium, manganese sulfate are 1:0.9～1.1:0.9～1.1:0.9～1.1 according to mol ratio.
Due to the utilization of technique scheme, the present invention compared with prior art has following advantages: the present inventionThe special anti-continuous cropping complexing agent of cucumber adopting, can be in solving soil-borne disease microbial continuous cropping problem,Can solve again the continuous cropping problem being caused by soil nutritional deficiency, simultaneously owing to having added phosphorus decomposing silicate-dissolving microbe bacterial manure,Having formed beneficial microbe colony at plant root, improved the micro-ecological environment of soil, is the growth of cucumberPhosphorus potassium nutrition element is provided. Use the special successive planting resistant agent of cucumber of the present invention can effectively reduce due to heavyThe underproduction that stubble problem causes, on average increases output more than 8%.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is further elaborated.
Embodiment 1
The special anti-continuous cropping complexing agent of a kind of cucumber, this complexing agent is by bacillus subtilis solid fungicide, Xie DianPowder bacillus solid fungicide, streptomyces microflavus solid fungicide, microbial-bacterial fertilizer phosphorus potassium complex fertilizer and trace unitElement additive is made according to the ratio mixture of mass ratio 20:12:10:57:1.
In this example, the bacillus subtilis solid fungicide in the special anti-continuous cropping complexing agent of this cucumber, solution starch budSpore bacillus solid fungicide and streptomyces microflavus solid fungicide are prepared from by following processing step:
The preparation of A, liquid seeds liquid
A1, by the bacillus subtilis, bacillus amyloliquefaciens that preserve and streptomyces microflavus original strain respectivelyWith after plate streak activation, the beef-protein medium (beef of picking list colony inoculation after sterilizingCream 5g, peptone 10g, sodium chloride 3g, the pH value of culture medium is controlled at 7.2～7.6, adds water and is settled to 1000ml)In, expanding to cultivate through test tube, triangular flask two-stage respectively and make liquid strain daughter bacteria liquid, condition of culture is rotating speed30 DEG C of 200r/min, cultivation temperature, cultivation cycle 24h;
The sterilising conditions of this beef-protein medium is that temperature is 121 DEG C, and the time is 30min.
A2, preparation koji tray culture medium, wherein, dry raw material according to mass percent by wheat bran 80%, form sedimentPowder 10.78%, corn flour 5%, soya-bean cake 4%, potassium nitrate 0.1%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate0.05%, sodium chloride 0.02% proportioning, adds water and mixes, and wherein material-water ratio is 1:1.1, is mixed with koji tray trainingSupport base, after sterilizing, on the koji tray culture medium of 10kg, inoculate respectively bacillus subtilis, separate starch gemma barThe seed culture fluid 500ml of bacterium, streptomyces microflavus is that 30 DEG C of temperature, humidity are 70% at condition of cultureUnder environment, cultivate 5 days;
The sterilising conditions of this koji tray culture medium is that temperature is 121 DEG C, and the time is 30min.
Koji tray samples before cultivating discharging, measures spore content with colony counting method, and spore content is not less than 4,000,000,000Individual/gram.
A3, after steps A 2 dischargings, stir koji tray and smashed, under 40 DEG C of conditions, be dried to moistureQuality percentage composition is 15%, makes respectively bacillus subtilis solid fungicide, bacillus amyloliquefaciens after pulverizingSolid fungicide, streptomyces microflavus solid fungicide.
It is raw that microbial-bacterial fertilizer phosphorus potassium complex fertilizer in this example is selected from Beijing favour symphysis thing agricultural high-tech Co., Ltd" favour is closed " board microbial bacterial agent producing.
Trace mineral supplement in this example is according to NY1428-2007 standard " trace element water-soluble fertilizer "Requirement is prepared, and wherein, the content of manganese, zinc, boron, the each element of molybdenum is the total matter of trace mineral supplement3% of amount. Wherein manganese, zinc, boron, the each element of molybdenum are selected from respectively manganese sulfate, zinc sulfate, boric acid, ammonium molybdate.
Embodiment 2
In this example, the special anti-continuous cropping complexing agent of cucumber is by bacillus subtilis solid fungicide, separates starch gemma barBacterium solid fungicide, streptomyces microflavus solid fungicide, microbial-bacterial fertilizer phosphorus potassium complex fertilizer and trace mineral supplementMake according to the ratio mixture of mass ratio 25:10:8:56.2:0.8.
Bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide and streptomyces microflavus in this exampleThe biological preparation process of solid fungicide is with embodiment 1, and its difference is: 1) beef extract-peptone training in steps A 1That supports base is formulated as beef extract 4.5g, peptone 10g, sodium chloride 2.5g, and the pH value of culture medium is controlled at7.2～7.6, add water and be settled to 1000ml; 2) in steps A 2, the dry raw material of koji tray culture medium be according toMass percent by wheat bran 78%, starch 14.78%, corn flour 4%, soya-bean cake 3%, potassium nitrate 0.1%,Dipotassium hydrogen phosphate 0.05%, magnesium sulfate 0.04%, sodium chloride 0.03% proportioning, material-water ratio is 1:1.2.
Microbial-bacterial fertilizer phosphorus potassium complex fertilizer in this example is selected from the production of Shi Nong bio tech ltd, Xiamen" good Tian Da " board microbial manure.
Trace mineral supplement in this example is according to NY1428-2007 standard " trace element water-soluble fertilizer "Requirement is prepared, and wherein, the content of manganese, zinc, boron, the each element of molybdenum is the total matter of trace mineral supplement3.5% of amount. Wherein manganese, zinc, boron, the each element of molybdenum are selected from respectively manganese sulfate, zinc chloride, boric acid, molybdic acidAmmonium.
Embodiment 3
In this example, the special anti-continuous cropping complexing agent of cucumber is by bacillus subtilis solid fungicide, separates starch gemma barBacterium solid fungicide, streptomyces microflavus solid fungicide, microbial-bacterial fertilizer phosphorus potassium complex fertilizer and trace mineral supplementMake according to the ratio mixture of mass ratio 18:10:12:59.8:1.2.
Bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide and streptomyces microflavus in this exampleThe biological preparation process of solid fungicide is with embodiment 1, and its difference is: 1) beef extract-peptone training in steps A 1That supports base is formulated as beef extract 5.5g, peptone 9.5g, sodium chloride 3g, and the pH value of culture medium is controlled at 7.5,Add water and be settled to 1000ml; 2), in steps A 2, the dry raw material of koji tray culture medium is according to quality percentageRatio is by wheat bran 80%, starch 13.78%, corn flour 3%, soya-bean cake 3%, potassium nitrate 0.09%, phosphoric acid hydrogenDipotassium 0.05%, magnesium sulfate 0.05%, sodium chloride 0.03% proportioning, material-water ratio is 1:1.23; 3) steps A 3In, after koji tray is smashed, under 45 DEG C of conditions, being dried to containing water quality percentage composition is to carry out powder after 10% againBrokenly make bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide, streptomyces microflavus solid bacteriumAgent.
Microbial-bacterial fertilizer phosphorus potassium complex fertilizer in this example is selected from the production of Jun De bio tech ltd, Shandong" bacterium obtains rich " board micro organism composite fertilizer.
Trace mineral supplement in this example is according to NY1428-2007 standard " trace element water-soluble fertilizer "Requirement is prepared, and wherein, the content of manganese, zinc, boron, the each element of molybdenum is the total matter of trace mineral supplement4% of amount. Wherein manganese, zinc, boron, the each element of molybdenum are selected from respectively manganese sulfate, zinc chloride, borax, ammonium molybdate.
Embodiment 4
In this example, negative control group is not for carrying out any processing, and medicine control group is dazomet processing, experimental groupRespectively by special cucumber anti-continuous cropping complexing agent (the special anti-continuous cropping of cucumber preparing in embodiment 1,2,3Complexing agent) before cucumber field planting, apply in planting pit equably, and be designated as respectively enforcement processed group 1, enforcement placeReason group 2, enforcement processed group 3, each test group is the planting experiment of Zuo Sange community respectively, plantation and growth periodBetween management and fertilising by carrying out according to conventional method. Several harvests commodity fruit is also used scales/electronic balance weighing, statisticsTotal output, the results are shown in Table.
In this example, selected kind is Shandong vegetables No. 21, and planting site is chosen in Suburbs of Shanghai and has planted 2 yearsThe warmhouse booth of cucumber.
The impact of the special anti-continuous cropping complexing agent of table 1 cucumber on continuous cropping cucumber output
Result by table 1 can find out, the special anti-continuous cropping complexing agent of cucumber can suppress because continuous cropping is ledThereby cause the problem of the cucumber disease underproduction, and its result is good compared with the result of chemicals (dazomet),Can improve the more than 8% of cucumber yield.
Above-described embodiment is only explanation technical conceive of the present invention and feature, and its object is to allow is familiar with this skillThe personage of art can understand content of the present invention and be implemented, and can not limit protection model of the present invention with thisEnclose, all equivalences that Spirit Essence is done according to the present invention change or modify, and all should be encompassed in protection of the present inventionIn scope.
Claims (7)
1. the special anti-continuous cropping complexing agent of cucumber, it is characterized in that, described complexing agent is to be made up according to the ratio mixture of mass ratio 18 ~ 25:8 ~ 12:8 ~ 12:50 ~ 65:0.8 ~ 1.2 of bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide, streptomyces microflavus solid fungicide, microbial-bacterial fertilizer phosphorus potassium complex fertilizer and trace mineral supplement.
2. the special anti-continuous cropping complexing agent of cucumber according to claim 1, is characterized in that, described bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide and streptomyces microflavus solid fungicide are prepared from by following processing step:
The preparation of A, liquid seeds liquid
A1, by bacillus subtilis, bacillus amyloliquefaciens and the streptomyces microflavus original strain preserved respectively with after plate streak activation, in the fluid nutrient medium of picking list colony inoculation after sterilizing, expand to cultivate through test tube, triangular flask two-stage respectively and make liquid strain daughter bacteria liquid, condition of culture is 28 ~ 30 DEG C of rotating speed 200 ~ 250r/min, cultivation temperature, cultivation cycle 24h;
Wherein, fluid nutrient medium used is beef-protein medium, to be made by the raw material of following mass percent: beef extract 0.45 ~ 0.55%, peptone 0.95 ~ 1.05%, sodium chloride 0.25 ~ 0.3%, surplus is water, the pH value of described culture medium is controlled at 7.2 ~ 7.6;
A2, the koji tray culture medium that the liquid strain daughter bacteria liquid of preparation in steps A 1 is inoculated into after sterilizing are cultivated, the inoculum concentration of liquid strain daughter bacteria liquid be after sterilizing koji tray culture medium quality 3 ~ 5%, condition of culture is 28 ~ 30 DEG C of temperature, humidity 50% ~ 70%, cultivation cycle 5 ~ 7 days, koji tray samples before cultivating discharging, measure spore content with colony counting method, spore content is not less than 4,000,000,000/gram;
Wherein, the culture medium that koji tray is cultivated is made up of the dry raw material of following mass percent:
Wheat bran 75 ~ 80%, starch 10 ~ 15%, corn flour 3 ~ 5%, soya-bean cake 3 ~ 4%, potassium nitrate 0.09 ~ 0.1%, dipotassium hydrogen phosphate 0.04 ~ 0.05%, magnesium sulfate 0.04 ~ 0.05%, sodium chloride 0.02 ~ 0.03%, in the preparation, material-water ratio is 1:1.1 to the culture medium that koji tray is cultivated: ~ 1.2;
A3, after steps A 2 dischargings, stir koji tray and smashed, under 40 ~ 45 DEG C of conditions, being dried to containing water quality percentage composition is 10 ~ 15%, makes respectively bacillus subtilis solid fungicide, bacillus amyloliquefaciens solid fungicide, streptomyces microflavus solid fungicide after pulverizing.
3. the special anti-continuous cropping complexing agent of cucumber according to claim 1, it is characterized in that, described microbial-bacterial fertilizer phosphorus potassium complex fertilizer is selected the GB20287-2006 standard of carrying out, 200,000,000/gram above biological potassium phosphorus or phosphorus potassium micro organism composite fertilizers of the effective bacterial content of microorganism.
4. according to the special anti-continuous cropping complexing agent of the cucumber described in claim 1 or 3, it is characterized in that, described microbial-bacterial fertilizer phosphorus potassium complex fertilizer is selected from the one in " good Tian Da " board microbial manure that " favour is closed " board microbial bacterial agent, Shi Nong bio tech ltd, Xiamen that Beijing favour symphysis thing agricultural high-tech Co., Ltd produces produce, " bacterium obtains rich " board micro organism composite fertilizer that Jun De bio tech ltd, Shandong produces.
5. the special anti-continuous cropping complexing agent of cucumber according to claim 1, is characterized in that, in described trace mineral supplement, the content of manganese, zinc, boron, the each element of molybdenum is all not less than 3% of described trace mineral supplement gross mass.
6. the special anti-continuous cropping complexing agent of cucumber according to claim 5, is characterized in that, in described trace mineral supplement, manganese element is selected from manganese sulfate, zinc element is selected from the one in zinc sulfate or zinc chloride, and boron element is selected from the one in borax or boric acid, and molybdenum element is selected from ammonium molybdate.
7. according to the special anti-continuous cropping complexing agent of the cucumber described in claim 5 or 6, it is characterized in that the ratio preparation that in described trace mineral supplement, borax or boric acid, zinc sulfate or zinc chloride, ammonium molybdate, manganese sulfate are 1:0.9 ~ 1.1:0.9 ~ 1.1:0.9 ~ 1.1 according to mol ratio.
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| CN108243652A (en) * | 2017-12-07 | 2018-07-06 | 山西省农业科学院生物技术研究中心 | A kind of method for treating seeds for alleviating quinoa continuous cropping obstacle |
| CN108727082A (en) * | 2018-07-10 | 2018-11-02 | 安徽六国化工股份有限公司 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
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| CN108727082A (en) * | 2018-07-10 | 2018-11-02 | 安徽六国化工股份有限公司 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
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Application publication date: 20160525 |