CN108727082A - A kind of bacillus bacterium solution stablizes store method and bio-feritlizer - Google Patents
A kind of bacillus bacterium solution stablizes store method and bio-feritlizer Download PDFInfo
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- CN108727082A CN108727082A CN201810750176.7A CN201810750176A CN108727082A CN 108727082 A CN108727082 A CN 108727082A CN 201810750176 A CN201810750176 A CN 201810750176A CN 108727082 A CN108727082 A CN 108727082A
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- bacillus
- bacterium solution
- bacillus bacterium
- store method
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to microorganisms technical field, it is specifically related to a kind of bacillus bacterium solution and stablizes store method and bio-feritlizer.Main technical schemes of the present invention：Bacillus bacterium solution stablizes store method：Added with soluble composite fertilizer in the bacillus bacterium solution.In addition the microbial manure that store method obtains is stablized by the bacillus bacterium solution.After the present invention in bacillus bacterium solution by adding soluble composite fertilizer stirring and dissolving, it can inhibit the viable bacteria in zymotic fluid and prevent the secondary fermentation of product bacterium, and it can prevent miscellaneous bacteria from growing simultaneously, it is rotten so as to prevent zymotic fluid from occurring, zymotic fluid stability is improved, that is, realizes bacillus bacterium solution room temperature preservation.The present invention is especially suitable for the preservations of production bacillus biological fertilizer field zymotic fluid or centrifugate of fermenting.
Description
Technical field
The invention belongs to microorganisms technical field, it is specifically related to a kind of bacillus bacterium solution and stablizes store method and life
Object fertilizer.
Background technology
Since zymotic fluid is not easy permanent preservation, it is usually the case that after one time fermentation, need in time to fermentation
Liquid is handled, especially hot summer, if zymotic fluid is handled not in time, is easily occurred rotten.Currently, zymotic fluid
Processing mode be timely carry out bacterium, liquid separation, mainly plate compression or continuous centrifugal separation by way of complete,
Or directly moisture is removed by the way of freeze-drying.These three modes, which are handled, is required for the time, plate compression or continuous
It is relatively short that formula centrifuges the time, but there are filtrate or centrifugate, and filtrate, there is also perishable to ask for centrifugate
Topic, is not suitable with room temperature preservation；And be lyophilized and need freeze dryer, less efficient, single freeze-drying amount is limited, difficult in batch production process
Exempt from can there is a problem of that zymotic fluid places what long generation was gone bad.
Invention content
In order to solve the above technical problem, the present invention provides a kind of bacillus bacterium solutions to stablize store method and bio-fertilizer
Material.
In order to achieve the object of the present invention, present invention employs following technical schemes：
A kind of bacillus bacterium solution stablizes store method, added with soluble composite fertilizer in the bacillus bacterium solution.
Further scheme：Nitrogenous fertilizer source is in urea, monoammonium phosphate, ammonium chloride, ammonium sulfate in the solubility composite fertilizer
Any one or several combinations, phosphate fertilizer source is monoammonium phosphate, any one of Diammonium phosphate (DAP) in the solubility composite fertilizer
Or several combination；The source of potash fertilizer is any one of potassium chloride, potassium sulfate or several groups in the solubility composite fertilizer
It closes.
Further scheme：Solubility composite fertilizer additive amount is the 5~25% of the bacillus bacterium solution gross mass.
Further scheme：The bacillus bacterium solution is zymotic fluid or fermentation centrifugate.
Further scheme：The bacillus bacterium solution includes bacillus subtilis, bacillus amyloliquefaciens, lichens gemma
In bacillus, bacillus megaterium, bacillus pumilus, Bacillus sphaericus, bacillus stearothermophilus, bacillus thuringiensis
Any one or several combinations.
It is a kind of to stablize the microbial manure that store method obtains by the bacillus bacterium solution.
The beneficial effects of the present invention are：
(1) after the present invention in bacillus bacterium solution by adding soluble composite fertilizer stirring and dissolving, can inhibit to ferment
Viable bacteria in liquid and the secondary fermentation for preventing product bacterium, and can prevent miscellaneous bacteria from growing simultaneously, so as to prevent from fermenting
Rotten, raising zymotic fluid stability, i.e. realization bacillus bacterium solution room temperature preservation occur for liquid.Especially suitable for producing bacillus
The preservation of biological fertilizer field zymotic fluid or centrifugate of fermenting.
(2) the additive amount control of the soluble composite fertilizer of the present invention bacillus living can be inhibited to grow and to gemma sheet
Body does not have killing effect, while the additive amount control of composite fertilizer can inhibit varied bacteria growing, to prevent zymotic fluid rotten.
(3) addition of the soluble composite fertilizer of the present invention can make bacillus bacterium solution be applied to field of planting, with solvable
Property composite fertilizer facilitates plant absorption and does not pollute the environment as stabilizer.
Specific implementation mode
More specific detail is made to technical solution of the present invention with reference to embodiment：
Embodiment 1
Fermentation of bacillus liquid 100Kg, soluble nitrogen phosphorus potassium complex fertilizer 13.8Kg are taken, wherein the composition of soluble composite fertilizer
For：Urea 2.2Kg, sodium chloride 2.3Kg, Diammonium phosphate (DAP) 2.5Kg, monoammonium phosphate 2.5Kg, potassium sulfate 2.0Kg, potassium chloride
2.3Kg).Then soluble nitrogen phosphorus potassium complex fertilizer is dissolved in fermentation centrifugate, is uniformly mixed, you can room temperature preservation or inspection
Packaging manufacture.
Embodiment 2
With embodiment 1, except that soluble nitrogen phosphorus potassium complex fertilizer 12.8Kg, wherein the ratio of components of soluble composite fertilizer
Example be：Ammonium chloride 4.5Kg, Diammonium phosphate (DAP) 4.5Kg, potassium chloride 3.8Kg.
Embodiment 3
With embodiment 2, except that soluble nitrogen phosphorus potassium complex fertilizer 25Kg, wherein the group of soluble composite fertilizer becomes：
Ammonium chloride 8.79Kg, Diammonium phosphate (DAP) 8.79Kg, potassium chloride 7.42Kg.
Embodiment 4
Fermentation of bacillus centrifugate 100Kg, soluble nitrogen phosphorus potassium complex fertilizer 10.8Kg are taken, wherein soluble composite fertilizer
Group becomes：Urea 3.6Kg, Diammonium phosphate (DAP) 3.6Kg, potassium sulfate 3.6Kg.
Embodiment 5
With embodiment 4, except that soluble nitrogen phosphorus potassium complex fertilizer 5.0Kg, wherein the group of soluble composite fertilizer becomes：
Urea 1.67Kg, Diammonium phosphate (DAP) 1.67Kg, potassium sulfate 1.67Kg.
It is that stability of the bacillus bacterium solution after preserving 6 months is as shown in table 1 below in above-described embodiment below：
Table 1
| Group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Stability | Do not go bad | Do not go bad | Do not go bad | Do not go bad | Do not go bad |
Claims (6)
1. a kind of bacillus bacterium solution stablizes store method, it is characterised in that：Added with solubility in the bacillus bacterium solution
Composite fertilizer.
2. bacillus bacterium solution as described in claim 1 stablizes store method, it is characterised in that：In the solubility composite fertilizer
Nitrogenous fertilizer source is any one of urea, monoammonium phosphate, ammonium chloride, ammonium sulfate or several combinations, the solubility composite fertilizer
Middle phosphate fertilizer source is any one of monoammonium phosphate, Diammonium phosphate (DAP) or several combinations；Potash fertilizer in the solubility composite fertilizer
Source is any one of potassium chloride, potassium sulfate or several combinations.
3. bacillus bacterium solution as described in claim 1 stablizes store method, it is characterised in that：The solubility composite fertilizer adds
Dosage is the 5~25% of the bacillus bacterium solution gross mass.
4. bacillus bacterium solution as described in claim 1 stablizes store method, it is characterised in that：The bacillus bacterium solution is
Zymotic fluid or fermentation centrifugate.
5. bacillus bacterium solution as described in claim 1 stablizes store method, it is characterised in that：In the bacillus bacterium solution
Including bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium, bacillus pumilus, spherical bud
Any one of spore bacillus, bacillus stearothermophilus, bacillus thuringiensis or several combinations.
6. a kind of being stablized the microbial manure that store method obtains by Claims 1 to 5 any one of them bacillus bacterium solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810750176.7A CN108727082A (en) | 2018-07-10 | 2018-07-10 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810750176.7A CN108727082A (en) | 2018-07-10 | 2018-07-10 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108727082A true CN108727082A (en) | 2018-11-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810750176.7A Pending CN108727082A (en) | 2018-07-10 | 2018-07-10 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039210A (en) * | 2015-06-29 | 2015-11-11 | 北京沃土天地生物科技有限公司 | Complex lively bacterial preparation as well as water soluble fertilizer and application thereof |
| CN105601449A (en) * | 2015-12-30 | 2016-05-25 | 顺达康（苏州）农业科技发展有限公司 | Special continuous cropping resisting complexing agent for cucumbers |
| CN106348884A (en) * | 2016-08-31 | 2017-01-25 | 五洲丰农业科技有限公司 | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer |
| FR3004448B1 (en) * | 2013-04-12 | 2017-12-08 | Polyor Sarl | SEPARATE BUT INTEGRAL PACKAGING OF STARTER FERTILIZER BASED ON BACTERIA |
| CN107814633A (en) * | 2017-12-01 | 2018-03-20 | 河北兴乔能源科技有限公司 | A kind of preparation method of efficient liquid bio-feritlizer |
-
2018
- 2018-07-10 CN CN201810750176.7A patent/CN108727082A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3004448B1 (en) * | 2013-04-12 | 2017-12-08 | Polyor Sarl | SEPARATE BUT INTEGRAL PACKAGING OF STARTER FERTILIZER BASED ON BACTERIA |
| CN105039210A (en) * | 2015-06-29 | 2015-11-11 | 北京沃土天地生物科技有限公司 | Complex lively bacterial preparation as well as water soluble fertilizer and application thereof |
| CN105601449A (en) * | 2015-12-30 | 2016-05-25 | 顺达康（苏州）农业科技发展有限公司 | Special continuous cropping resisting complexing agent for cucumbers |
| CN106348884A (en) * | 2016-08-31 | 2017-01-25 | 五洲丰农业科技有限公司 | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer |
| CN107814633A (en) * | 2017-12-01 | 2018-03-20 | 河北兴乔能源科技有限公司 | A kind of preparation method of efficient liquid bio-feritlizer |
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Application publication date: 20181102 |