CN107216223A - A kind of liquid composite microorganism fertilizer and preparation method thereof - Google Patents
A kind of liquid composite microorganism fertilizer and preparation method thereof Download PDFInfo
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- CN107216223A CN107216223A CN201710227937.6A CN201710227937A CN107216223A CN 107216223 A CN107216223 A CN 107216223A CN 201710227937 A CN201710227937 A CN 201710227937A CN 107216223 A CN107216223 A CN 107216223A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The present invention provides a kind of liquid composite microorganism fertilizer, count by weight, the liquid composite microorganism fertilizer includes 1 10 parts of strain, 10 80 parts of amino acid fermentation waste liquid, 20 60 parts of inorganic fertilizer raw material, 5 10 parts of potassium fulvate, 15 parts of borax, 0.5 5 parts of EDTA zinc, 0.05 0.5 parts of auxiliary agent, 0.1 4 parts of pH adjusting agent, 0.1 1.5 parts of suspension thickener, 5 20 parts of water.Also provide the method for preparing above-mentioned fertilizer, both Fertilizer combination of the invention liquid fertilizer and microbial manure advantage, comprehensive nutrition using this patent fertilizer, can reduce composite fertilizer's consumption, improve utilization rate of fertilizer, raising crop yield and quality.
Description
Technical field
The invention belongs to fertilizer field, and in particular to a kind of liquid composite microorganism fertilizer and preparation method thereof.
Background technology
At present, China is in agricultural production, and some areas land management is improper, and soil organic matter content is reduced year by year, leads
The serious uneven, soil hardening of plant nutrient substance and aging phenomenon are caused, the development of China's agricultural is seriously govern.
Microbial manure is to cause crop to obtain a kind of product of specific fertilizer effect with the vital movement of microorganism, is agriculture
One kind of fertilizer is used in industry production.It is in the existing nearly 50 years history of China.Its effect is mainly conditioning soil；Reduce chemical fertilizer
Usage amount；Improve quality of agricultural product；Strengthen crop resistance and resistance；Strengthen integrated campaign of the crop to poor environment simultaneously
Ability.Experiment proves that ecological organic fertilier can make nitrate salts content of vegetable averagely reduce by 48.3~87.7%, and nitrogen, phosphorus, potassium content are carried
High 5~20% etc..Microbial manure, to people, animal, Environmental security, nontoxic, is a kind of environment-friendly type fertilizer.
In amino acids production, bulk fermentation waste water causes the severe contamination to environment from plant emissions.It is rich in industrial wastewater
Containing a large amount of water-soluble organic matters, the content of organic matter is higher than 20%, and total nutrient accounts for 6%.These waste water are unprocessed to be directly discharged into water
Body, causes environmental pollution and the destruction of the ecosystem while wasting resource.This technology is sent out using the amino acid of low cost
Ferment waste liquid does microbe carrier and prepares liquid composite microorganism fertilizer, be comprehensive nutrition, long shelf-life, do not destroy soil texture,
Fertilizer efficiency stabilization and the good liquid microbe fertilizer of the feature of environmental protection.
CN201410771848.4 discloses a kind of liquid microbe fertilizer, the component containing following parts by weight：Withered grass bud
30~50 parts of spore bacillus bacterium solution, 25-40 parts of bacillusmusilaginosiengineering bacterium solution, 20~35 parts of inorganic fertilizer, 15~30 parts of feces of livestock and poultry,
5~15 parts of malto-oligosaccharide, 5~10 parts of molasses, 1~5 part of magnesium sulfate, 2-6 parts of borax, 4-8 parts of zinc sulfate, filler 0.05~
1 part, 0.01~0.05 part of gibberellin, 0.01~0.05 part of the basic element of cell division, 40~55 parts of water.
CN 201310260550.2 discloses organic microbial manure of a kind of romaine lettuce and preparation method thereof, and it prepares former
Material include following weight than composition：It is 4-7 parts of streptomyces aureus, 0.6-0.9 parts of bacillus thuringiensis, 3-5 parts of ferment bacterium, useless
900-1100 parts of liquid precipitate thing, 240-380 parts of inorganic fertilizer, 45-55 parts of trace element, 25-32 parts of adhesive, stabilizer 0.5-
0.7 part, steady 0.1-0.3 parts of auxiliary agent of effect.
CN 201210003707.9 discloses a kind of microbial manure, by the microbial fermentation solution of following percent by volume
Composition：Described microorganism is photosynthetic bacteria, aztobacter sp, phosphorus decomposing bacillus megaterium, bacillusmusilaginosiengineering, put
Line bacterium, lactic acid bacteria；Described microbial fermentation solution volume ratio is：1-15：2-10：5-9：1-8：2-10：3-8.
It is that compound microorganism ferments liquid and embedding medium are constituted that CN 201010590247.5, which discloses composite microbiological fertilizer,
Based on volume parts ratio, compound microorganism ferments liquid: embedding medium=1: 5-1: 4；The compound microorganism ferments liquid is false for production protein
Silk yeast, streptococcus lactis, lactobacillus acidophilus, the co-incubation thing of bacillus subtilis and Slow-growing soybean rhizobia, raw fat are consolidated
Nitrogen spirillum, swamp Rhodopseudomonas, bacillus megaterium, colloid bacillus cereus and azotobacter chroococcum single nutrient solution it is compound and
Into.
Above-mentioned patent discloses each strain as the application of microbial manure, but does not utilize the distinctive function of amino acid
Cooperate with and play a role with microorganism mutual cooperation.
The content of the invention
To solve the above problems, the invention provides a kind of liquid composite microorganism fertilizer and preparation method thereof, specific skill
Art scheme is as follows：
A kind of liquid composite microorganism fertilizer, is counted by weight, and the liquid composite microorganism fertilizer includes strain 1-
10 parts, 10-80 parts of amino acid fermentation waste liquid, 20-60 parts of inorganic fertilizer raw material, 5-10 parts of potassium fulvate, 1-5 parts of borax, EDTA- zinc
0.5-5 parts, 0.05-0.5 parts of auxiliary agent, 0.1-4 parts of pH adjusting agent, 0.1-1.5 parts of suspension thickener, 5-20 parts of water.
It is preferred that, count by weight, the liquid composite microorganism fertilizer includes 2-5 parts of strain, amino acid fermentation gives up
30-55 parts of liquid, 25-50 parts of inorganic fertilizer raw material, 2-4 parts of borax, 1-2 parts of EDTA- zinc, 0.11-0.23 parts of auxiliary agent, pH adjusting agent
0.5-2 parts, 0.2-1.0 parts of suspension thickener, 10-15 parts of water.
It is preferred that, the amino acid fermentation waste liquid includes Threonine Fermentation waste liquid, fermentation waste liquid of lysine, valine fermentation
Waste liquid.
It is preferred that, the solid content weight content of the amino acid fermentation waste liquid is 30%-50%.
It is preferred that, the strain is bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, solution starch gemma
Bacillus.
It is preferred that, the inorganic fertilizer raw material includes 5-15 parts of urea, 5-10 parts of ammonium nitrate, 10-15 parts of potassium dihydrogen phosphate, sulphur
Sour potassium 5-10 parts.
It is preferred that, the auxiliary agent includes photosynthetic element, the basic element of cell division, Nafusaku, it is preferred that described including cell division
0.01~0.03 part, photosynthetic plain 0.1~0.2 part of element.
It is preferred that, it is fine that the suspension thickener includes dispersant, sodium alginate, xanthans, carboxymethyl cellulose, carboxyethyl
One or more in dimension element.
The present invention also provides a kind of preparation method of aforesaid liquid composite microbiological fertilizer, and the preparation method includes as follows
Step：
(1) amino acid fermentation waste liquid is added into reactor, starts reaction；
(2) water, inorganic fertilizer raw material etc. are added, stirs and grinds；
(3) potassium fulvate, borax, EDTA zinc, suspension thickener are sequentially added into mixed solution；
(4) pH adjusting agent is added, regulation reaction solution pH is 5.7-6.3, is slowly added to strain, auxiliary agent, circular response 4 is small
When, reaction solution is finished product.
Beneficial effects of the present invention：
(1) the recycling application of amino acid fermentation waste liquid, rich in soluble small molecular organic matter, promotes crop root life
It is long, soil organic matter content is improved, good environment is provided for microorganism growth；
(2) addition of potassium fulvate conditioner, with organic matter nutrition in waste liquid, Nutrient medium is provided for microbial growth
Plinth, protects microbial survival condition, and the stimulation from water and inorganic salts endangers.
(3) simple production process, reaction condition is gentle, and cost is low；
Fluid present invention meets effect of microbial manure：
(1) present invention is using amino acid fermentation waste liquid as main organic principle, and waste water utilization economizes on resources, Environmental Safety.It is useless
The molten organic matter of micromolecular water is rich in liquid, organic nutrition is provided directly to plant, promotes crop root growth, it is ensured that rainy weather
The accumulation of the carbohydrate of plant and nutrient absorption；Overcast and rainy still to absorb nutrition, growing way of keeping fit, effectively prevention are planted
The generation that strain is wilted.
(2) small molecule humic acid conditioner is added with, is acted synergistically with microorganism secretion thing, can improved soil granule knot
Structure；Strengthen water conservation, the fertilizer-preserving ability of soil.The soil organism, rehabilitating soil microbiota, the micro- life of soil protection can also be improved
State environment, improves soil hardening, saline and alkaline situation.
(3) romaine lettuce of this patent fertilizer is applied：1) picking time shifts to an earlier date 3-5 days；2) slow seedling is fast after plant is transplanted seedlings, growth period without
Withered phenomenon；3) more competing product fertilizer output increased 12.3%；4) romaine lettuce is fresh and tender, and the more competing product of chlorophyll improve 11.2%.
(4) advantage, comprehensive nutrition, using this patent both of the invention Fertilizer combination liquid fertilizer and microbial manure
Fertilizer, can reduce composite fertilizer's consumption, improve utilization rate of fertilizer, improve crop yield and quality, with very high social benefit,
Economic benefit and ecological benefits, are worthy of popularization.
Embodiment
The embodiment to the present invention is described further below：
The preparation of the liquid composite microorganism fertilizer of embodiment 1
(1) Threonine Fermentation waste liquid 45kg, wherein the solid content weight in Threonine Fermentation waste liquid are added into reactor
Content is 50%, starts reaction；
(2) water 12kg, urea 10kg, ammonium nitrate 7kg, potassium dihydrogen phosphate 12kg, potassium sulfate 7kg are added, stirs and grinds；
(3) potassium fulvate 5kg, borax 3kg, EDTA zinc 1.5kg, carboxymethyl cellulose are sequentially added into mixed solution
0.5kg, xanthans 0.5kg；
(4) pH adjusting agent (sodium hydroxide solution) is added, regulation reaction solution pH is 6.1, is slowly added to strain, cell division
Plain 0.02kg, photosynthetic plain 0.15kg, wherein strain are bacillus subtilis 0.5kg, bacillus megaterium 0.5kg, gel-shaped bud
Spore bacillus 1kg, bacillus amyloliquefaciens 1kg, the microbial inoculum viable count is >=2 hundred million/gram, circular response 4 hours, reaction solution is
Finished product.
Embodiment 2
(1) fermentation waste liquid of lysine 50kg, the solid content weight wherein in fermentation waste liquid of lysine are added into reactor
Content is 45%, starts reaction；
(2) water 14kg, urea 8kg, ammonium nitrate 9kg, potassium dihydrogen phosphate 10kg, potassium sulfate 6kg are added, stirs and grinds；
(3) potassium fulvate 5kg is sequentially added into mixed solution, borax 2.5kg, EDTA zinc 1.8kg, sodium alginate
0.5kg, carboxyethyl cellulose 0.5kg；
(4) pH adjusting agent (sodium hydroxide solution) is added, regulation reaction solution pH is 6.0, is slowly added to strain, cell division
Plain 0.02kg, photosynthetic plain 0.15kg, wherein strain are bacillus subtilis 1.5kg, bacillus megaterium 0.5kg, gel-shaped bud
Spore bacillus 0.5kg, bacillus amyloliquefaciens 0.5kg, the microbial inoculum viable count be >=2 hundred million/gram, circular response 4 hours, reaction solution
As finished product.
Embodiment 3
(1) valine fermented waste fluid 55kg, the solid content weight wherein in fermentation waste liquid of lysine are added into reactor
Content is 30%, starts reaction；
(2) water 14kg, urea 15kg, ammonium nitrate 10kg, potassium dihydrogen phosphate 15kg, potassium sulfate 10kg are added, stirs and grinds
It is broken；
(3) potassium fulvate 5kg is sequentially added into mixed solution, borax 2kg, EDTA zinc 1.2kg, sodium alginate
0.4kg, xanthans 0.2kg；
(4) pH adjusting agent (sodium hydroxide solution) is added, regulation reaction solution pH is 6.0, is slowly added to strain, cell division
Plain 0.15kg, photosynthetic plain 0.12kg, wherein strain are bacillus subtilis 0.8kg, bacillus megaterium 1.2kg, gel-shaped bud
Spore bacillus 0.5kg, bacillus amyloliquefaciens 0.5kg, the microbial inoculum viable count be >=2 hundred million/gram, circular response 4 hours, reaction solution
As finished product.
Comparative example
Comparative example 1：Compared with Example 1, change Threonine Fermentation waste liquid 45kg into alcoholic fermentation waste liquor 45kg, concentrate wine
Smart fermented waste fluid makes its solid content weight content identical with threonine, is 50%, other conditions are same as Example 1, is prepared into
To contrast liquid composite microbial fertilizer 1.
Comparative example 2：Compared with Example 1, potassium fulvate, other conditions and the phase of embodiment 1 are added without in step (3)
Together, contrast liquid composite microbial fertilizer 2 is prepared.
Comparative example 3：Compared with Example 1, the strain in step (4) is changed to bacillus subtilis 3kg, that is, is changed to single product
The microorganism planted, other conditions are same as Example 1, prepare contrast liquid composite microbial fertilizer 3.
Comparative example 4：Compared with Example 1, the strain in step (4) be changed to strain for bacillus subtilis 0.005kg,
Bacillus megaterium 0.05kg, bacillusmusilaginosiengineering 0.01kg, bacillus amyloliquefaciens 0.01kg, i.e., by the addition of strain
With the scale smaller of amino acid, make that strain amount is very few, amount of amino acid is excessive, and other conditions are same as Example 1, prepare pair
Than liquid composite microbial fertilizer 4.
Effect example 1
Contrast liquid prepared by the liquid composite microbial fertilizer prepared in above-described embodiment 1-3 and comparative example 1-4
Composite microbe fertilizer is applied on romaine lettuce crop (5 mu), before the sowing of romaine lettuce crop field, using the Kilograms Per Square Meter of fertilizer 0.5,
Sowed after being well mixed with seedbed soil, first site preparation and fertilization before field planting, average every mu of 5 kilograms of applying liquid composite microbe fertilizer is calculated
Fertilizer yield increase rate, detects the chlorophyll raising amount of Chinese leaf, chlorophyll is measured with chlorophyll detector, and calculates romaine lettuce
Picking time full production capacity.Specific such as table 1：
Table 1
| Sequence number | Picking time (number of days in advance) | Yield increase rate (%) | Chlorophyll incrementss (%) |
| Embodiment 1 | 5 | 12.3 | 11.2 |
| Embodiment 2 | 4 | 13.4 | 12.1 |
| Embodiment 3 | 5 | 12.7 | 11.8 |
| Comparative example 1 | 1 | 5.1 | 4.5 |
| Comparative example 2 | 0 | 3.2 | 3.8 |
| Comparative example 3 | 1 | 4.6 | 4.2 |
| Comparative example 4 | 0 | 3.8 | 3.7 |
Using the romaine lettuce of this patent fertilizer：Picking time shifts to an earlier date 4-5 days；Romaine lettuce output increased more than 12.3%；Romaine lettuce is fresh and tender,
The more competing product of chlorophyll improve more than 11.2%.
Effect example 2
Above-described embodiment 1-3 liquid composite microbial fertilizers prepared and comparative example 1-4 the contrast liquid prepared are answered
The detection method reference NYT 2321-2013 of the viable count in microorganism fertilizer are closed, and by after liquid composite microbial fertilizer 12 months
Its viable count is detected, the viable count in each liquid composite microbial fertilizer is contrasted；Detect dataware table 2：
Table 2
| Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Viable count (hundred million/gram) | 0.27 | 0.12 | 0.08 | 0.18 | 0.01 |
| Viable count (hundred million/gram, after 12 months) | 0.268 | 0.04 | 0.03 | 0.04 | 0.002 |
From Table 2, it can be seen that the viable count in bio-fertilizer prepared by comparative example is implemented less than technical solution of the present invention
Example 1 prepare bio-fertilizer in viable count, and place 12 months after, in embodiment 1 bio-fertilizer viable count maintain be basically unchanged,
And contrast the viable count in 1-4 bio-fertilizers and decline substantially, microorganism is deposited in bio-fertilizer prepared by this explanation technical solution of the present invention
Motility rate is higher, amino acid powder and plus potassium fulvate be combined as microorganism provide preferably existence nutrient environment, make microbial survival
Phase is longer.
The announcement and teaching of book according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and changed.Therefore, the invention is not limited in embodiment disclosed and described above, to the one of invention
A little modifications and changes should also be as falling into the scope of the claims of the present invention.Although in addition, being used in this specification
Some specific terms, but these terms are merely for convenience of description, do not constitute any limitation to the present invention.
Claims (9)
1. a kind of liquid composite microorganism fertilizer, it is characterised in that count by weight, the liquid composite microorganism fertilizer bag
Include 1-10 parts of strain, 10-80 parts of amino acid fermentation waste liquid, 20-60 parts of inorganic fertilizer raw material, 5-10 parts of potassium fulvate, borax 1-5
Part, 0.5-5 parts of EDTA- zinc, 0.05-0.5 parts of auxiliary agent, 0.1-4 parts of pH adjusting agent, 0.1-1.5 parts of suspension thickener, water 5-20
Part.
2. liquid composite microorganism fertilizer according to claim 1, it is characterised in that count by weight, the liquid
Composite microbiological fertilizer includes 2-5 parts of strain, 30-55 parts of amino acid fermentation waste liquid, 25-50 parts of inorganic fertilizer raw material, borax 2-4
Part, 1-2 parts of EDTA- zinc, 0.11-0.23 parts of auxiliary agent, 0.5-2 parts of pH adjusting agent, 0.2-1.0 parts of suspension thickener, water 10-15
Part.
3. liquid composite microorganism fertilizer according to claim 1, it is characterised in that the amino acid fermentation waste liquid includes
Threonine Fermentation waste liquid, fermentation waste liquid of lysine, valine fermented waste fluid.
4. liquid composite microorganism fertilizer according to claim 1, it is characterised in that the amino acid fermentation waste liquid is consolidated
Shape thing weight content is 30%-50%.
5. liquid composite microorganism fertilizer according to claim 1, it is characterised in that the strain is bacillus subtilis
Bacterium, bacillus megaterium, bacillusmusilaginosiengineering, bacillus amyloliquefaciens.
6. liquid composite microorganism fertilizer according to claim 1, it is characterised in that the inorganic fertilizer raw material includes urea
5-15 parts, 5-10 parts of ammonium nitrate, 10-15 parts of potassium dihydrogen phosphate, 5-10 parts of potassium sulfate.
7. liquid composite microorganism fertilizer according to claim 1, it is characterised in that the auxiliary agent includes photosynthetic plain, thin
Born of the same parents' mitogen, Nafusaku, it is preferred that it is described including 0.01~0.03 part of the basic element of cell division, photosynthetic plain 0.1~0.2 part.
8. liquid composite microorganism fertilizer according to claim 1, it is characterised in that the suspension thickener includes scattered
One or more in agent, sodium alginate, xanthans, carboxymethyl cellulose, carboxyethyl cellulose.
9. a kind of preparation method according to any one of the claim 1-8 liquid composite microorganism fertilizers, it is characterised in that institute
Preparation method is stated to comprise the following steps：
(1) amino acid fermentation waste liquid is added into reactor, starts reaction；
(2) water, inorganic fertilizer raw material etc. are added, stirs and grinds；
(3) potassium fulvate, borax, EDTA zinc, suspension thickener are sequentially added into mixed solution；
(4) pH adjusting agent is added, regulation reaction solution pH is 5.7-6.3, is slowly added to strain, auxiliary agent, circular response 4 hours, instead
Answer liquid as finished product.
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Cited By (14)
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| CN107935668A (en) * | 2017-10-13 | 2018-04-20 | 深圳市芭田生态工程股份有限公司 | It is a kind of to be used to improve blade face preparation of crop anti-adversity and preparation method thereof |
| CN107986882A (en) * | 2017-12-25 | 2018-05-04 | 陈益德 | A kind of energy conservation and environmental protection organic suspension liquid compound fertilizer and preparation method thereof |
| CN108002924A (en) * | 2017-12-28 | 2018-05-08 | 深圳市芭田生态工程股份有限公司 | A kind of cold-resistant microbial liquid fertilizer and preparation method thereof |
| CN108559719A (en) * | 2018-03-28 | 2018-09-21 | 华北制药集团爱诺有限公司 | A kind of liquid microbe bacterial manure |
| CN109206250A (en) * | 2018-11-13 | 2019-01-15 | 广东新维生物科技股份有限公司 | A kind of fertilizer solving the problems, such as soil root and preparation method |
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| CN110373363A (en) * | 2019-08-22 | 2019-10-25 | 福建三炬生物科技股份有限公司 | A kind of concentration microbial inoculum of bacillusmusilaginosiengineering and its preparation method and application |
| CN111995459A (en) * | 2020-07-23 | 2020-11-27 | 华南农业大学 | Organic water-retaining suspension liquid compound fertilizer and preparation method thereof |
| CN112194538A (en) * | 2020-10-10 | 2021-01-08 | 四川龙蟒福生科技有限责任公司 | Gibberellin bacterial residue treatment method and liquid fertilizer containing gibberellin bacterial residue treatment product |
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| CN114437965A (en) * | 2021-12-31 | 2022-05-06 | 河北科技师范学院 | Peanut rhizosphere microbial strain and method for improving and repairing soil by using biological bacterial fertilizer thereof |
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