A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa
Technical field
The present invention relates to a kind of microbial bacterial agent and its application, more particularly to a kind of containing the micro- of Paenibacillus polymyxa
Bacteria agent and its application, belong to microbial technology field.
Background technology
The crumb structure of soil is the important indicator of soil fertility, and the destruction of soil granular structure causes soil water-retaining, protected
Fertile ability and permeability reduce, and cause soil hardening.Soil granular structure is that electronegative soil clay particle and organic matter pass through band
What the polyvalent cation connection of positive electricity formed.Soil particle is connected into bulky grain by polyvalent cation soil hardening in the form of key bridge
Form soil granular structure.Cation in soil is based on the calcium of divalent, magnesium ion, when phosphate fertilizer is excessively applied into soil, phosphorus
The cation such as calcium, magnesium combines to form insoluble phosphate in phosphate anion and soil in fertilizer, that is, wastes phosphate fertilizer, destroy again
Soil granular structure, cause soil hardening.
Same crops are solely planted for a long time, are easy to cause the glutinous weight of the soil texture in farmland, and then cause soil
Hardened, crumb structure is seriously damaged;If continuing kind of a same crops, the root growth of crops is slow, dead rate height.
How to overcome long-term continuous cropping to cause soil hardening to cause plantling strain dead, turn into the important research class in this area
Topic.
The content of the invention
The present invention is solves the shortcomings of the prior art, there is provided a kind of microbial bacterial agent containing Paenibacillus polymyxa and
It is applied, to realize following goal of the invention:
(1)Improve soil hardening problem caused by long-term continuous cropping;(2)Reduce dead of the seedling strain of the Planting Crops in hardened soil
Rate.
In order to solve the above technical problems, using following technical scheme:
A kind of microbial bacterial agent containing Paenibacillus polymyxa, described microbial inoculum include following raw material components:Dregs of beans, mushroom residue,
Stalk, polyglutamic acid, poly-aspartic-acid, sodium selenite, boric acid, ferrous sulfate, bacillus subtilis bacterium solution, lichens gemma bar
Bacterium bacterium solution and bacillus amyloliquefaciens bacterium solution.
Described microbial inoculum also includes following raw material components:Paenibacillus polymyxa bacterium solution and He Yu fields Dell Ford bacterium bacterium
Liquid.
Described microbial inoculum includes the raw material components of following parts by weight:Dregs of beans 24-27 parts, mushroom residue 10-12 parts, stalk 6.5-
7.5 parts, polyglutamic acid 1.5-2.2 parts, poly-aspartic-acid 1.3-1.6 parts, sodium selenite 1.1-1.4 parts, boric acid 0.4-0.6
Part, ferrous sulfate 0.4-0.6 parts, bacillus subtilis bacterium solution 2-3 parts, Bacillus licheniformis liquid 2.5-3.5 parts, solution starch
Bacillus bacterium solution 2.5-3.5 parts, Paenibacillus polymyxa bacterium solution 0.8-1.2 parts, He Yu fields Dell Ford bacterium bacterium solution 4.4-
4.7 part.
Described microbial inoculum includes the raw material components of following parts by weight:25 parts of dregs of beans, 11 parts of mushroom residue, 7 parts of stalk, polyglutamic
2 parts of acid, 1.5 parts of poly-aspartic-acid, 1.2 parts of sodium selenite, 0.5 part of boric acid, 0.5 part of ferrous sulfate, bacillus subtilis bacterium
2 parts of liquid, 2.5 parts of Bacillus licheniformis liquid, 3 parts of bacillus amyloliquefaciens bacterium solution, 1 part of Paenibacillus polymyxa bacterium solution,
4.5 parts of He Yu fields Dell Ford bacterium bacterium solution.
Described dregs of beans is the graininess dregs of beans crushed, and wherein particle diameter is that 0.1-0.15mm dregs of beans particle accounts for 30%, particle diameter
14% is accounted for for 0.07-0.09 mm dregs of beans particle, particle diameter is that 0.03-0.05mm dregs of beans particle accounts for 40%.
It is 300 mesh that described mushroom residue, which is crushed to fineness,;
Described stalk is:The sorghum green grass or young crops straw of not long sorghum fringe, moisture are dried in 15-18%, it is 0.8-1mm to be crushed to particle diameter
Graininess.
Described bacillus subtilis bacterium solution:Viable bacteria containing bacillus subtilis 5 × 109CFU/g;
Described Bacillus licheniformis liquid:Viable bacteria containing bacillus licheniformis 11.2 × 109CFU/g;
Described bacillus amyloliquefaciens bacterium solution:Viable bacteria containing bacillus amyloliquefaciens 7.5 × 109CFU/g;
Described Paenibacillus polymyxa bacterium solution:Viable bacteria containing Paenibacillus polymyxa 22 × 109CFU/g;
Described He Yu fields Dell Ford bacterium bacterium solution:The Dell of field containing He Yu Ford bacterium viable bacteria 17 × 109CFU/g。
A kind of application of the microbial bacterial agent containing Paenibacillus polymyxa, described microbial bacterial agent move for plantling strain
Used when planting the plot into soil hardening.
The amount of application of described microbial bacterial agent is 200-220g/ caves;Plantlet of transplant survival rate is significantly improved after.
Compared with prior art, the beneficial effects of the invention are as follows:
(1)The present invention substantially improves soil hardening problem caused by long-term continuous cropping;Using 4 months, the soil weight was from 3.5-3.6g/
㎝3It is reduced to 1.68 g/ ㎝3, soil porosity improved to 58.5% from 28-29.5, and soil granular structure mean size improves
To 3.5-3.6mm;
(2)The present invention is substantially reducing at dead rate of seedling strain of Planting Crops in hardened soil, and 1.5-5.5% is reduced to from 31%.
Embodiment
Bacillus subtilis preserving number CICC10071 of the present invention, described bacillus licheniformis preserving number are ACCC
02002, the bacillus amyloliquefaciens preserving number CICC10036, the Paenibacillus polymyxa preserving number CICC10288, institute
Shu Heyu fields Dell Ford bacterium preserving number CICC10492, above-mentioned strain derives from commercially available.
A kind of microbial bacterial agent containing Paenibacillus polymyxa of embodiment 1
25 parts of dregs of beans, 11 parts of mushroom residue, 7 parts of stalk, 2 parts of polyglutamic acid, 1.5 parts of poly-aspartic-acid, 1.2 parts of sodium selenite,
0.5 part of boric acid, 0.5 part of ferrous sulfate, 2 parts of bacillus subtilis bacterium solution, 2.5 parts of Bacillus licheniformis liquid, solution starch bud
3 parts of spore bacillus bacterium solution, 1 part of Paenibacillus polymyxa bacterium solution, 4.5 parts of He Yu fields Dell Ford bacterium bacterium solution;
Described dregs of beans is the graininess dregs of beans crushed, and wherein particle diameter is that 0.1-0.15mm dregs of beans particle accounts for 30%, and particle diameter is
0.07-0.09 mm dregs of beans particle accounts for 14%, and particle diameter is that 0.03-0.05mm dregs of beans particle accounts for 40%;
It is 300 mesh that described mushroom residue, which is crushed to fineness,;
Described stalk is:The sorghum green grass or young crops straw of not long sorghum fringe, moisture are dried in 15-18%, it is 0.8-1mm to be crushed to particle diameter
Graininess;
Described bacillus subtilis bacterium solution:Viable bacteria containing bacillus subtilis 5 × 109CFU/g;
Described Bacillus licheniformis liquid:Viable bacteria containing bacillus licheniformis 11.2 × 109CFU/g;
Described bacillus amyloliquefaciens bacterium solution:Viable bacteria containing bacillus amyloliquefaciens 7.5 × 109CFU/g;
Described Paenibacillus polymyxa bacterium solution:Viable bacteria containing Paenibacillus polymyxa 6 × 109CFU/g;
Described He Yu fields Dell Ford bacterium bacterium solution:The Dell of field containing He Yu Ford bacterium viable bacteria 10 × 109CFU/g。
Preparation method:First dregs of beans, mushroom residue and stalk are well mixed and obtain solid material, then by bacillus subtilis
Bacterium solution, Bacillus licheniformis liquid, bacillus amyloliquefaciens bacterium solution, Paenibacillus polymyxa bacterium solution and He Yu fields Dell Ford bacterium
Bacterium solution is sprayed into solid material successively, after stirring, sequentially adds polyglutamic acid, poly-aspartic-acid, sodium selenite, boric acid
And ferrous sulfate, microbial bacterial agent is made.
The single factor analysis experiment of the dregs of beans granularity of embodiment 2
Microbial bacteria is prepared using a kind of microbial bacterial agent containing Paenibacillus polymyxa described in embodiment 1 and preparation method thereof
Agent, the granularity for only changing dregs of beans carry out embodiment 2-4;Specifically it is shown in Table 1;
The granularity of the dregs of beans of table 1
The dregs of beans particles that embodiment 2-4 is respectively adopted prepare microbial bacterial agent, and embodiment 1-4 microbial inoculum is under the conditions of 25 DEG C
Total viable bacteria storage rate of microbial inoculum is detected after placing 20 days, 20 days;Concrete outcome is shown in Table 2;
The viable bacteria storage rate of the microbial bacterial agent of table 2
As seen from the above table, the viable bacteria storage rate highest of microorganism, it is preferred embodiment under the conditions of the granularity of embodiment 1.
The single factor analysis experiment of the Paenibacillus polymyxa content of embodiment 5
Microbial bacteria is prepared using a kind of microbial bacterial agent containing Paenibacillus polymyxa described in embodiment 1 and preparation method thereof
Agent, the strain viable bacteria amount for only changing Paenibacillus polymyxa bacterium solution carry out embodiment 5-8;Specifically it is shown in Table 3;
The strain viable bacteria amount of the Paenibacillus polymyxa bacterium solution of table 3
The single factor analysis experiment of the He Yu fields Dell Ford bacterial content of embodiment 9
Microbial bacteria is prepared using a kind of microbial bacterial agent containing Paenibacillus polymyxa described in embodiment 1 and preparation method thereof
Agent, the strain viable bacteria amount for only changing He Yu fields Dell Ford bacterium bacterium solution carry out embodiment 9-12;Specifically it is shown in Table 4;
The strain viable bacteria amount of the He Yu fields Dell Ford bacterium bacterium solution of table 4
The microbial bacterial agent application effect of embodiment 13 detects
It is applied to long-term continuous cropping plantation eggplant using embodiment 5-12 microbial bacterial agent to cause in the plot of soil hardening, specifically
Using as follows:
It is more than season to plant eggplant 10 for described plot, continuous continuous cropping, and soil granular structure is seriously damaged, hardened serious, soil
For earth crumb structure mean size in 0.2-0.4mm, the soil weight is 3.5-3.6g/ ㎝3, soil porosity 28-29.5%;
Eggplant seedling strain is transplanted to above-mentioned plot, dead rate of seedling strain is higher;Planted using embodiment 5-12 microbial bacterial agent, significantly
The seedling strain death rate is reduced, concrete application method is:During transplanting, seedling strain retains 4-5 piece true leaves, is sprinkled into the seedling cave of every seedling strain
200g microbial bacterial agents, after being mixed with soil, seedling strain is put into, pours into 100ml water, then earthing fills and leads up seedling cave;Plantation 1 month
Afterwards, seedling strain survival condition is counted;Specifically it is shown in Table 5;
Table 5
From table 5,
(1)The single factor analysis experiment of Paenibacillus polymyxa content:
It is 5-8 to be related to embodiment, is as a result shown, embodiment 7 is preferred embodiment;
(2)The single factor analysis experiment of He Yu fields Dell Ford bacterial content:
It is 9-12 to be related to embodiment, is as a result shown, embodiment 10 is preferred embodiment;
Synthesis obtains, and Paenibacillus polymyxa number of viable is preferably 22 × 10 in Paenibacillus polymyxa bacterium solution9CFU/g, crane
Yu Tian Dell Ford bacterium bacterium solution Zhong Heyu fields Dell Ford bacterium number of viable is preferably 17 × 109CFU/g。
A kind of microbial bacterial agent containing Paenibacillus polymyxa of embodiment 14
25 parts of dregs of beans, 11 parts of mushroom residue, 7 parts of stalk, 2 parts of polyglutamic acid, 1.5 parts of poly-aspartic-acid, 1.2 parts of sodium selenite,
0.5 part of boric acid, 0.5 part of ferrous sulfate, 2 parts of bacillus subtilis bacterium solution, 2.5 parts of Bacillus licheniformis liquid, solution starch bud
3 parts of spore bacillus bacterium solution, 1 part of Paenibacillus polymyxa bacterium solution, 4.5 parts of He Yu fields Dell Ford bacterium bacterium solution;
Described dregs of beans is the graininess dregs of beans crushed, and wherein particle diameter is that 0.1-0.15mm dregs of beans particle accounts for 30%, and particle diameter is
0.07-0.09 mm dregs of beans particle accounts for 14%, and particle diameter is that 0.03-0.05mm dregs of beans particle accounts for 40%;
It is 300 mesh that described mushroom residue, which is crushed to fineness,;
Described stalk is:The sorghum green grass or young crops straw of not long sorghum fringe, moisture are dried in 15-18%, it is 0.8-1mm to be crushed to particle diameter
Graininess;
Described bacillus subtilis bacterium solution:Viable bacteria containing bacillus subtilis 5 × 109CFU/g;
Described Bacillus licheniformis liquid:Viable bacteria containing bacillus licheniformis 11.2 × 109CFU/g;
Described bacillus amyloliquefaciens bacterium solution:Viable bacteria containing bacillus amyloliquefaciens 7.5 × 109CFU/g;
Described Paenibacillus polymyxa bacterium solution:Viable bacteria containing Paenibacillus polymyxa 22 × 109CFU/g;
Described He Yu fields Dell Ford bacterium bacterium solution:The Dell of field containing He Yu Ford bacterium viable bacteria 17 × 109CFU/g。
Preparation method:First dregs of beans, mushroom residue and stalk are well mixed and obtain solid material, then by bacillus subtilis
Bacterium solution, Bacillus licheniformis liquid, bacillus amyloliquefaciens bacterium solution, Paenibacillus polymyxa bacterium solution and He Yu fields Dell Ford bacterium
Bacterium solution is sprayed into solid material successively, after stirring, sequentially adds polyglutamic acid, poly-aspartic-acid, sodium selenite, boric acid
And ferrous sulfate, microbial bacterial agent is made.
Through testing, cause the ground of soil hardening applied to long-term continuous cropping plantation eggplant using the microbial bacterial agent of the present embodiment
In block, specific method is identical with embodiment 13;As a result show, dead rate of eggplant is only 1.5%;After plantation 4 months, the soil weight
From 3.5-3.6g/ ㎝3It is reduced to 1.68 g/ ㎝3, soil porosity improved to 58.5% from 28-29.5, and soil granular structure is put down
Equal size is improved to 3.5-3.6mm.
In summary, compared with existing microbial bacterial agent, continuous cropping plant is planted using microbial bacterial agent of the present invention, hence it is evident that
Improve soil granular structure, improved soil is hardened.
In addition to specified otherwise, ratio of the present invention is mass ratio, and described percentage is mass percent.
Finally it should be noted that:The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can be with
Technical scheme described in foregoing embodiments is modified, or equivalent substitution is carried out to which part technical characteristic.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements made etc., it should be included in the guarantor of the present invention
Within the scope of shield.Citing described above for best mode for carrying out the invention, wherein the part do not addressed in detail is this area
The common knowledge of those of ordinary skill;Protection scope of the present invention is defined by the content of claim, any based on the present invention's
Technical inspiration and the equivalent transformation carried out, also within protection scope of the present invention.