CN116391726A - Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits - Google Patents
Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits Download PDFInfo
- Publication number
- CN116391726A CN116391726A CN202310604073.0A CN202310604073A CN116391726A CN 116391726 A CN116391726 A CN 116391726A CN 202310604073 A CN202310604073 A CN 202310604073A CN 116391726 A CN116391726 A CN 116391726A
- Authority
- CN
- China
- Prior art keywords
- hhb
- zwp15
- fermentation
- biocontrol microbial
- delftia lacustris
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 59
- 230000000443 biocontrol Effects 0.000 title claims abstract description 48
- 244000298697 Actinidia deliciosa Species 0.000 title claims abstract description 46
- 235000009436 Actinidia deliciosa Nutrition 0.000 title claims abstract description 46
- 150000001875 compounds Chemical class 0.000 title claims abstract description 31
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 27
- 230000000694 effects Effects 0.000 claims abstract description 24
- 230000005764 inhibitory process Effects 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 14
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 11
- 230000002195 synergetic effect Effects 0.000 claims abstract description 8
- 241001600129 Delftia Species 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 47
- 230000004151 fermentation Effects 0.000 claims description 47
- 235000010633 broth Nutrition 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 16
- 239000000306 component Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 230000000813 microbial effect Effects 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 230000002265 prevention Effects 0.000 claims description 11
- 239000002054 inoculum Substances 0.000 claims description 6
- 229920001592 potato starch Polymers 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 3
- 244000105624 Arachis hypogaea Species 0.000 claims description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 3
- 235000018262 Arachis monticola Nutrition 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 235000020232 peanut Nutrition 0.000 claims description 3
- 239000002552 dosage form Substances 0.000 claims description 2
- 239000012681 biocontrol agent Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- 239000003814 drug Substances 0.000 abstract description 5
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 26
- 201000010099 disease Diseases 0.000 description 19
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 11
- 239000000725 suspension Substances 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 238000011081 inoculation Methods 0.000 description 8
- 230000003042 antagnostic effect Effects 0.000 description 7
- 230000003902 lesion Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000193830 Bacillus <bacterium> Species 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- 239000004563 wettable powder Substances 0.000 description 5
- 235000009434 Actinidia chinensis Nutrition 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 230000003449 preventive effect Effects 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- JJLJMEJHUUYSSY-UHFFFAOYSA-L Copper hydroxide Chemical compound [OH-].[OH-].[Cu+2] JJLJMEJHUUYSSY-UHFFFAOYSA-L 0.000 description 2
- 239000005750 Copper hydroxide Substances 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 229910001956 copper hydroxide Inorganic materials 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- PVTHJAPFENJVNC-MHRBZPPQSA-N kasugamycin Chemical compound N[C@H]1C[C@H](NC(=N)C(O)=O)[C@@H](C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H]1O PVTHJAPFENJVNC-MHRBZPPQSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 239000012880 LB liquid culture medium Substances 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 108700009559 Pseudomonas syringae HrpZ Proteins 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000010242 baoji Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000004709 eyebrow Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000004562 water dispersible granule Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a compound biocontrol microbial inoculum for preventing and treating kiwi fruit bacterial canker. The strain of the compound biocontrol microbial inoculum isDelftia lacustrisZWP15Bacillus proteolyticus Hhb.019，Delftia lacustrisZWP15Bacillus proteolyticusHhb.019 is compatible and has synergistic inhibition effect on pathogenic bacteria of kiwifruit bacterial canker. The field control effect of the compound biocontrol microbial inoculum is 73.89 percent, which is superior to the control effect of single strainAnd has the control effect equivalent to that of the common medicines on the market. The compound biocontrol microbial inoculum has low preparation cost, is safer than the traditional control method, is nontoxic and harmless to the environment, and can be industrially produced.
Description
Technical Field
The invention belongs to the technical field of microbial resource utilization, relates to application of microorganisms and fermentation products thereof in plant disease control, and in particular relates to a compound biocontrol microbial inoculum for controlling kiwi fruit bacterial canker.
Background
The bacterial canker of the kiwifruit is formed by pathogenic variants of pseudomonas syringae kiwifruitPseudomonas syringae pv. actinidiae，Psa) The damage to the kiwi fruit is caused, the damage is caused, the influence period is long, the epidemic frequency is high, the prevention and control difficulty is high, and the healthy development of the kiwi fruit industry is severely restricted. At present, the prevention and treatment of bacterial canker of kiwi fruits mainly adopts chemical prevention and treatment, copper preparations and antibiotics are the most commonly used and better-effective medicaments in the prevention and treatment of canker, the use effectiveness of the two medicaments is limited along with the increasing prominence of the problem of 3R, and the use of streptomycin on kiwi fruits is forbidden in some European countries.
The biological control is a plant disease and pest control method which integrates the advantages of safety, high efficiency, difficult generation of drug resistance and the like, and meets the requirements of agricultural development in a new period. The biocontrol microbial agent prepared by microorganisms is an important component for biological control, but the biocontrol microbial agent has popularization and application values only with the characteristics of adapting to field production practice, such as broad antibacterial spectrum, high control efficiency, stable control efficiency and the like. The single-strain biocontrol microbial inoculum has strong environmental dependence and single action mechanism, cannot exert biocontrol potential to the greatest extent, is unfavorable for application and popularization, and can more efficiently control plant diseases and insect pests by utilizing the synergistic effect of multiple biocontrol mechanisms after compounding multiple biocontrol microbial inoculum so as to reduce cost and improve benefit.
At present, the excellent effects exhibited by the compound biocontrol bacteria are shown in tobacco black shank, fusarium wilt, banana leaf-penetrating nematode disease, apple ring rot and soybean root rotThe disease, jujube black spot, gray mold and other plant diseases are reported successively. Aiming at the pathogenic variation of the kiwi fruits from pseudomonas syringaePsa) The prevention and treatment of the bacterial canker of the kiwi fruits are not mature in technology and widely applied compound biocontrol bactericides.
Disclosure of Invention
The invention aims to solve the technical problems: the chemical agent or single strain biocontrol microbial inoculum is difficult to realize the efficient and safe control of the kiwi fruit bacterial canker; the copper preparation is still the main control of bacterial canker of kiwi fruits, and the available biocontrol resources are deficient.
In order to solve the problems in the prior art, the invention explores a new thought for preventing and controlling plant diseases, namely, the novel thought is scientifically compounded with biocontrol bacteria to exert the synergistic effect of various biocontrol mechanisms. The method can not only reduce the dependence of single biocontrol bacteria on the environment and participate in disease control more efficiently, but also provide new biocontrol product materials for the biological control of kiwi fruit bacterial canker.
Based on the technical thought, the invention firstly determines the compatibility of the kiwi fruit bacterial canker antagonistic bacteria combination, and then determines the kiwi fruit bacterial canker pathogenic bacteria of the combination on the basis of being compatiblePsaFinally, the control effect of the antagonistic bacteria combination on kiwi fruit bacterial canker is measured through in vitro and field control experiments
The invention provides a compound biocontrol microbial agent. The compound biocontrol microbial inoculum is used for preventing and treating kiwi fruit bacterial canker, and the adopted compound strain isDelftia lacustrisZWP15Bacillus proteolyticusHhb.019. The invention is obtained by the plate opposite and Psa inhibition tests,Delftia lacustrisZWP15Bacillus proteolyticusHhb.019 is compatible and has synergistic inhibition effect on pathogenic bacteria of kiwifruit bacterial canker. The effective components of the compound biocontrol microbial inoculum are as followsDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusCombination of hhb.019 fermentation broths. The invention utilizes the method of vacuum penetration of the isolated leaves and wound inoculation of the isolated branches to make sure that the compound biocontrol microbial inoculum is used for treating bacterial canker of kiwi fruitsPreventing effect.
Further, the present invention usesDelftia lacustrisThe preservation number of ZWP15 strain is CGMCC No. 2331Bacillus proteolyticusThe preservation number of Hhb.019 is CGMCC No.24797. Both strains were purchased from the China general microbiological culture Collection center (China Committee for culture Collection).
As one of the preferred embodiments of the compound biocontrol microbial inoculum, the invention comprises the following components in volume ratioDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusThe combination ratio of Hhb.019 fermentation broths is 1:1.
The invention further discloses the strainDelftia lacustris ZWP15、Bacillus proteolyticusThe fermentation medium composition and process parameters of hhb.019.
Specifically, the preparation ofDelftia lacustrisThe culture medium components of the ZWP15 fermentation broth are as follows: the weight percentage of potato starch is 0.571, the cotton seed cake powder is 0.5, the sodium chloride is 0.5, the dipotassium hydrogen phosphate is 0.25, and the balance is water; the fermentation parameters are as follows: temperature 24 ℃, rotational speed 205 r/min, initial pH value 7.0, theDelftia lacustrisZWP15 inoculum size 2%, fermentation duration 20.196 h.
Specifically, the preparation ofBacillus proteolyticusThe culture medium components of the Hhb.019 fermentation broth are: 1.0% of potato starch, 2.509% of peanut cake powder, 0.5% of sodium chloride and the balance of water; the fermentation parameters are as follows: temperature 28 ℃, rotating speed 234 r/min, initial pH value 7.0, theBacillus proteolyticusHhb.019 inoculum size 1.183% and fermentation time 40 h.
Based on the technical idea of the invention, a person skilled in the art can combine the prior art with the followingDelftia lacustrisZWP15 fermentation broth, describedBacillus proteolyticusThe Hhb.019 fermentation broth is compounded according to a specific proportion (preferably 1:1), and the compounded biocontrol microbial agent is processed into an agriculturally acceptable dosage form by adding an agriculturally used auxiliary agent or carrier and then is applied.
In addition, the invention discloses application of the compound biocontrol microbial inoculum in preventing and treating kiwi fruit bacterial canker. The compound biocontrol microbial agentThe strain isDelftia lacustrisZWP15Bacillus proteolyticus Hhb.019，Delftia lacustrisZWP15Bacillus proteolyticusHhb.019 is compatible and has synergistic inhibition effect on pathogenic bacteria of kiwifruit bacterial canker. Further, the effective components of the compound biocontrol microbial inoculum are as followsDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusCombination of hhb.019 fermentation broths. In terms of volume ratio, theDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusThe combination ratio of Hhb.019 fermentation broths is 1:1. Related toDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusThe process for preparing the hhb.019 fermentation broth is described above.
Compared with the prior art, the compound biocontrol microbial inoculum for preventing and treating kiwi fruit bacterial canker has the following beneficial effects or advantages:
the invention firstly determines the compatibility of different strain combinations, then determines the inhibition effect on pathogenic bacteria on the basis of definitely being compatible, and finally combines the in-vitro and field prevention effects to provide the compound biocontrol microbial inoculum for preventing and treating kiwi fruit bacterial canker. The theoretical method provides a technical approach for developing and preparing the plant disease and insect pest biocontrol microbial inoculum.
StrainDelftia lacustrisZWP15Bacillus proteolyticusHhb.019 is derived from healthy kiwi fruit rhizosphere soil, and is nontoxic and harmless to human and livestock, crops and ecological environment. The optimized fermentation medium has the advantages of cheap and easily obtained components, simple and easy operation of the fermentation method, low preparation cost of fermentation liquor, safe components and capability of meeting the requirement of large-scale production.
The invention obtains the strain through screeningDelftia lacustrisZWP15Bacillus proteolyticusHhb.019 has good compatibility and is pathogenic bacteria for kiwi fruit bacterial cankerPsaAn antagonistic combination with a strong M228 inhibition effect. The prevention and treatment test shows that the compound biocontrol microbial inoculum can be directly used for biological prevention and treatment of kiwi fruit bacterial canker, the prevention and treatment effect of the compound biocontrol microbial inoculum (10 times liquid of fermentation liquor) on kiwi fruit bacterial canker is 73.89%, and the compound biocontrol microbial inoculum and the commercial 6% kasugamycin wettable powder are 100 times liquid andthe control effect of the 100 times liquid of 46% copper hydroxide wettable powder is equivalent. The compound biocontrol microbial inoculum provided by the invention is an excellent kiwi fruit bacterial canker biological control resource.
Drawings
FIG. 1 shows the strainDelftia lacustrisZWP15Bacillus proteolyticusCompatibility determination of hhb.019. ZWP15 indicates the strainDelftia lacustrisZWP15, hhb.019 shows strainBacillus proteolyticusHhb.019, ZWP15+Hhb.019 is expressed in the strain containingDelftia lacustrismu.L of ZWP15 was added dropwise to the plateBacillus proteolyticusBacterial suspension of Hhb.019 strain, hhb.019+ZWP15 is shown in the strain-containing strainBacillus proteolyticusmu.L of Hhb.019 was added dropwise to the plateDelftia lacustrisBacterial suspension of ZWP15 strain.
FIG. 2 shows the strainDelftia lacustris ZWP15、Bacillus proteolyticusHhb.019 and combinations thereof against kiwi fruit bacterial canker pathogensPsaM228 in-dish inhibition results.
FIG. 3 shows the strainDelftia lacustris ZWP15、Bacillus proteolyticusHhb.019 and combination thereof, after vacuum infiltration inoculation by an in vitro leaf disc, against bacterial canker pathogens of kiwi fruitsPsaPreventive effect of M228.
FIG. 4 shows the strainDelftia lacustris ZWP15、Bacillus proteolyticusHhb.019 and combinations thereof to kiwi bacterial canker pathogens after inoculation of ex vivo shootsPsaPreventive effect of M228.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the following examples. It should be understood that the detailed description and specific examples are intended for purposes of illustration only and are not intended to limit the scope of the invention.
In the context of the various embodiments of the present invention,Delftia lacustristhe culture medium for activating ZWP15 is a PDA plate;Bacillus proteolyticusthe culture medium used for Hhb.019 activation is LB plate; pathogenic bacteria of bacterial canker of kiwifruitPsaMedium for M228 activationIs an LB plate.
If no special description exists, the culture conditions of the bacterial liquid are 28 ℃ and 220 r/min; the preparation method of the bacterial suspension comprises centrifuging the bacterial solution at 8000 r/min for 2 min, centrifuging with sterile water, washing twice, and re-suspending the bacterial suspension.
Example 1
Method for streaking preserved kiwi fruit bacterial canker antagonistic bacteriaDelftia lacustrisZWP15Bacillus proteolyticusHhb.019 was inoculated as a test strain onto a plate, respectively. The strain ZWP15 is cultured for 2-3 days at 28 ℃ by using a PDA culture medium and the strain Hhb.019 is cultured by using an LB culture medium, so that an activated strain to be tested is obtained.
And respectively inoculating the strain ZWP15 and the strain Hhb.019 to be detected into LB liquid culture medium, and carrying out shaking culture for 16-18 h at the constant temperature of 28 ℃ and 220 r/min by a shaking table. The cell concentration was adjusted to 1×10 using an ultraviolet spectrophotometer 8 CFU/mL（OD 600 =1.0), the strain liquid to be tested is obtained.
And (3) when the PDA culture medium is cooled to 45 ℃, mixing the prepared bacterial liquid with the PDA culture medium according to the proportion of 1:9, pouring the mixture into a flat plate, and dripping 10 mu L of bacterial liquid to be detected into the center of the culture medium after the mixture is dried. All strains were tested as test strains and challenge strains in two-by-two crossover. Repeating the treatment for 3 times, airing, placing in a 28 ℃ incubator, culturing for 2-5 d, and judging the compatibility of the two strains by observing the existence and the size of the inhibition zone, namely, judging whether the two strains co-grow. The inhibition zone is not obvious or smaller, namely the representative compatibility is better. As shown in figure 1 of the drawings,Delftia lacustrisZWP15Bacillus proteolyticusHhb.019 combination (marked as BD 18) has unobvious inhibition zone and good compatibility.
Example 2
Preparation of strains separatelyPsaM228, ZWP15 and Hhb.019 bacterial liquid. Bacterial solutions of the bacterial strains ZWP15 and Hhb.019 in the antagonistic bacterial combination BD18 with good compatibility are mixed together according to the ratio of 1:1 by using a bacteriostasis circle method, and after uniform mixing, the concentration of the bacterial solution is adjusted to 1 multiplied by 10 by using a spectrophotometer 8 CFU/mL. Cooling PDA culture medium to about 45deg.C, diluting pathogenic bacteriaPsa Mixing M228 bacterial liquid and PDA culture medium in the volume ratio of 1:9, pouring into a plate, and airingPunching (diameter d= mm) the center point of the culture medium after drying, respectively adding 10 mu L of bacteria liquid to be tested (ZWP 15 bacteria liquid, hhb.019 bacteria liquid and BD18 mixed bacteria liquid) into the small holes, repeating each group of treatment for 3 times, airing, placing in a 28 ℃ incubator for culturing for 2-3 d, and measuring the diameter of a bacteriostasis ring by using a cross method, wherein the diameter is shown in figure 2.
The measured diameter of the inhibition zone of the combination BD18 is 33.10+/-0.85 and mm, the diameter of the inhibition zone of the strain ZWP15 is 25.33+/-0.29 and mm, and the diameter of the inhibition zone of the strain Hhb.019 is 24.33+/-0.76 and mm. The Duncan's new complex pole difference method tests prove that the diameter of the combined BD18 inhibition zone is obviously larger than that of a single colony (p < 0.05), and the combined BD18 has obvious synergistic effect.
Example 3
Collecting healthy newly-grown fully-spread leaves with uniform size and consistent growth vigor from different kiwi fruit potted seedlings, performing surface disinfection (soaking in 0.6% NaClO solution for 6-8 min, washing with sterile water until no pungent smell exists, placing on filter paper for airing), and preparing a leaf disc by using a sterile puncher (diameter d=11 mm), wherein the main veins are avoided.
Preparation of strains separatelyPsaBacterial suspensions M228, ZWP15, hhb.019 and BD18 (mixing ratio 1:1), the concentration of the bacterial suspension was adjusted to 1X 10 4 CFU/mL was used for infestation. Sterile water is used as a negative control,Psam228 bacterial suspension is used as positive control, a leaf disc is placed in bacterial liquid, vacuum infiltration is carried out (0.1 MPa,10 s and 3 times), antagonistic bacteria ZWP15, hhb.019 and BD18, 24 and h are respectively inoculated first, and thenPsaM228 bacterial suspension.
The leaf discs are washed by sterile water, the water on the surfaces of the leaf discs is sucked by sterile filter paper, she Zhengmian is clung downwards to 0.8% water agar plates, 10-15 leaf discs are arranged on each water agar plate, and each treatment is repeated for 3 times.
The leaf discs were placed in a climatic incubator for moisture culture (photoperiod L/D:18 h/6 h; diurnal temperature: 16 ℃ C./10 ℃ C.; relative humidity: 90%) and after 4 days of inoculation, the leaf disc disease condition was observed and photographed for recording, the disease spot area was measured using imageJ software, and the preventive effect was calculated using the following formula.
Preventive effect = (control plaque area-treated plaque area)/control plaque area×100%
As shown in FIG. 3, the combination BD18, 24, h was inoculated prior to pathogenic bacteria inoculationPsaAt M228, leaf surface lesions were 9.86% in duty cycle, significantly lower than 21.97% of single species ZWP15, 18.06% of Hhb.019 and 54.46% of positive control. Calculated that the disease spots were prevented by the combination BD18 of 81.72% which is higher than 59.67% of ZWP15 and 66.78% of Hhb.019 of the single strain, there was a significant difference (p<0.05）。
Example 4
Collecting branches of current-year-old healthy kiwi fruits, cutting the branches into branches with the length of 10 cm after surface sterilization (the method is the same as that of example 3), and sealing the two ends by paraffin.
Preparation of strains separatelyPsaBacterial suspensions of M228, ZWP15, HHB.019 and BD18 (mixing ratio 1:1) (method same as example 3), the bacterial suspension concentration was adjusted to 1X 10 8 CFU/mL was used for inoculation. Sterile water is used as negative control, pathogenic bacteriaPsaM228 is a positive control, a wound (1 mm wide to phloem) is manufactured by using a sterile blade, 10 mu L of bacterial liquid is dripped into the wound, antagonistic bacteria ZWP15, hhb.019 and BD18, 24 h are inoculated firstly, and then inoculation is carried outPsaM228 bacterial suspension, 15 shoots per group were treated.
After the bacterial liquid permeated into the branches, the branches were placed in a climatic incubator for moisture culture (conditions are the same as in example 3), the disease condition was observed after inoculation of 20 d, the length of the lesions was measured and the results were recorded, and the preventive effect was calculated using the following formula. The results are shown in FIG. 4.
Preventive effect = (control lesion length-treatment lesion length)/control lesion length x 100%
For the combined BD18 group, the average lesion length was 1.50 mm, lower than 4.67 mm of strain hhb.019, 1.53 mm of strain ZWP15, and significantly lower than 13.6 mm of the positive control.
The combined BD18 has 88.97% of prevention effect on isolated branch lesions, which is higher than 88.73% of single strain ZWP15, and is significantly better than 65.69% (p) of strain Hhb.019<0.05). As shown by the experimental results, the combination BD18 can be used for treating bacterial canker pathogenic bacteria of kiwi fruitsPsaThe expansion of M228 plays a significant role in inhibition.
Example 5
The test sites of the field control effect are the Shanxi Baoji city and eyebrow county, yingfirst Zhenqian village (34 degrees 11 '15' N,107 degrees 44 '15' E), the orchard cultivation management level and the soil fertility are medium, and the cultivation conditions of all test communities are uniform. Peripheral land area kiwi fruit bacterial peach canker is highly developed. The tested plants are 4-5-year-old Xu Xiang kiwi fruit trees, and the growth vigor is consistent. The medicine is applied 1 time after fruit picking in 2021 autumn (middle and late 10 months), before leaf falling in winter (upper 11 months) and before flowering in spring (upper 3 months in the next year) and 3 times in total. The application adopts a shower drying method, and the application amount is 1.0 per plant L; each group was treated with 20 strains, repeated 3 times, 60 total strains. At 2022, 15/03, the number of the plants to be treated was investigated and recorded, and the disease rate and the control effect were calculated according to the following formula, and the results are shown in table 1.
Disease rate = number of disease plants/total number of investigation ×100%
Control effect= (control disease rate-treatment disease rate)/control disease rate×100%
Table 1, the field control effect of different medicaments on kiwi fruit bacterial canker
As can be seen from Table 1, the 10-time liquid of the compound biocontrol microbial inoculum BD18 fermentation liquid has a control effect of 73.89% on bacterial canker of kiwi fruit in the field, which is superior to 67.42% of 10-time liquid of ZWP15 fermentation liquid in a control group and 63.03% of 10-time liquid of Hhb.019 fermentation liquid, and is significantly superior to 53.28% of 100-time liquid of commercial bacillus subtilis wettable powder. The composition has no significant difference (p < 0.05) with 75.31% of 100-time liquid of 46% copper hydroxide water dispersible granule and 79.01% of 100-time liquid of 6% kasugamycin wettable powder and 73.46% of 100-time liquid of bacillus amyloliquefaciens wettable powder. The compound biocontrol microbial agent BD18 provided by the invention can be used for preventing and treating bacterial canker of kiwi fruits in fields.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments obtained without inventive effort by a person skilled in the art, which are related deductions and substitutions made by the person skilled in the art under the condition of the inventive concept, are within the scope of protection of the present invention.
Claims (10)
1. The application of the compound biocontrol microbial inoculum in the prevention and treatment of kiwi fruit bacterial canker is characterized in that the strain of the compound biocontrol microbial inoculum isDelftia lacustrisZWP15Bacillus proteolyticus Hhb.019，Delftia lacustrisZWP15Bacillus proteolyticusHhb.019 is compatible and has synergistic inhibition effect on pathogenic bacteria of kiwifruit bacterial canker.
2. The use according to claim 1, wherein the effective components of the compounded biocontrol agent areDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusCombination of hhb.019 fermentation broths.
3. The use according to claim 2, characterized in that the following are given in terms of volume ratioDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusThe combination ratio of Hhb.019 fermentation broths is 1:1.
4. Use according to claim 2 or 3, characterized in that the preparation of the saidDelftia lacustrisThe culture medium components of the ZWP15 fermentation broth are as follows: the weight percentage of potato starch is 0.571, the cotton seed cake powder is 0.5, the sodium chloride is 0.5, the dipotassium hydrogen phosphate is 0.25, and the balance is water;
the fermentation parameters are as follows: temperature 24 ℃, rotational speed 205 r/min, initial pH value 7.0, theDelftia lacustrisZWP15 inoculum size 2%, fermentation duration 20.196 h.
5. Use according to claim 2 or 3, characterized in that the preparation of the saidBacillus proteolyticusThe culture medium components of the Hhb.019 fermentation broth are: potato starch in mass percent1.0 percent, 2.509 percent of peanut cake powder, 0.5 percent of sodium chloride and the balance of water;
the fermentation parameters are as follows: temperature 28 ℃, rotating speed 234 r/min, initial pH value 7.0, theBacillus proteolyticusHhb.019 inoculum size 1.183% and fermentation time 40 h.
6. The compound biocontrol microbial agent is characterized by being used for preventing and treating bacterial canker of kiwi fruits, and the strain isDelftia lacustrisZWP15Bacillus proteolyticus Hhb.019，Delftia lacustrisZWP15Bacillus proteolyticusHhb.019 is compatible and has synergistic inhibition effect on pathogenic bacteria of kiwifruit bacterial canker, and the effective components of the compound biocontrol microbial inoculum are as followsDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusCombination of hhb.019 fermentation broths.
7. The compound biocontrol microbial agent of claim 6, wherein, based on volume ratio, the following is presentDelftia lacustrisZWP15 fermentation brothBacillus proteolyticusThe combination ratio of Hhb.019 fermentation broths is 1:1.
8. The compounded biocontrol microbial agent of claim 6 or 7, wherein the preparation of theDelftia lacustrisThe culture medium components of the ZWP15 fermentation broth are as follows: the weight percentage of potato starch is 0.571, the cotton seed cake powder is 0.5, the sodium chloride is 0.5, the dipotassium hydrogen phosphate is 0.25, and the balance is water;
the fermentation parameters are as follows: temperature 24 ℃, rotational speed 205 r/min, initial pH value 7.0, theDelftia lacustrisZWP15 inoculum size 2%, fermentation duration 20.196 h.
9. The compounded biocontrol microbial agent of claim 6 or 7, wherein the preparation of theBacillus proteolyticusThe culture medium components of the Hhb.019 fermentation broth are: 1.0% of potato starch, 2.509% of peanut cake powder, 0.5% of sodium chloride and the balance of water;
the fermentation parameters are as follows: temperature of 28 DEG CSpeed 234 r/min, initial pH 7.0, theBacillus proteolyticusHhb.019 inoculum size 1.183% and fermentation time 40 h.
10. The compounded biocontrol microbial agent of claim 6, wherein said compounded biocontrol microbial agent is processed into an agriculturally acceptable dosage form for administration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310604073.0A CN116391726B (en) | 2023-05-26 | 2023-05-26 | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310604073.0A CN116391726B (en) | 2023-05-26 | 2023-05-26 | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116391726A true CN116391726A (en) | 2023-07-07 |
| CN116391726B CN116391726B (en) | 2023-09-05 |
Family
ID=87020100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310604073.0A Active CN116391726B (en) | 2023-05-26 | 2023-05-26 | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116391726B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116987648A (en) * | 2023-09-28 | 2023-11-03 | 西北农林科技大学深圳研究院 | Bacillus pumilus and application thereof in kiwi fruit canker prevention and treatment |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320364A (en) * | 2013-07-03 | 2013-09-25 | 湖南农业大学 | Delftia sp. and application thereof to repairing butachlor phytotoxicity |
| CN103980047A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof |
| CN103980026A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer capable of promoting growth of rice seedling roots and preparation method thereof |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
| CN107475155A (en) * | 2017-09-15 | 2017-12-15 | 黑龙江宝基农业科技股份有限公司 | The soil remediation composite bacteria agent and its soil remediation method for soil fertilizer medicine residual of degrading |
| CN107841474A (en) * | 2017-11-24 | 2018-03-27 | 浙江大学 | A kind of pond life Delftiatsuruhatensis and its application in rice green smut preventing and treating |
| CN109355222A (en) * | 2018-11-06 | 2019-02-19 | 上海交通大学 | There is the bacillus of antagonism to rice leaf spot bacteria and separate and apply |
| CN110669687A (en) * | 2019-09-06 | 2020-01-10 | 华南农业大学 | Application of bacillus proteolicus in preventing and treating pathogenic bacteria and diseases depending on quorum sensing signal molecules |
| CN114736828A (en) * | 2022-04-27 | 2022-07-12 | 西北农林科技大学深圳研究院 | Biocontrol strain and application thereof in plant disease control |
-
2023
- 2023-05-26 CN CN202310604073.0A patent/CN116391726B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320364A (en) * | 2013-07-03 | 2013-09-25 | 湖南农业大学 | Delftia sp. and application thereof to repairing butachlor phytotoxicity |
| CN103980047A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof |
| CN103980026A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer capable of promoting growth of rice seedling roots and preparation method thereof |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
| CN107475155A (en) * | 2017-09-15 | 2017-12-15 | 黑龙江宝基农业科技股份有限公司 | The soil remediation composite bacteria agent and its soil remediation method for soil fertilizer medicine residual of degrading |
| CN107841474A (en) * | 2017-11-24 | 2018-03-27 | 浙江大学 | A kind of pond life Delftiatsuruhatensis and its application in rice green smut preventing and treating |
| CN109355222A (en) * | 2018-11-06 | 2019-02-19 | 上海交通大学 | There is the bacillus of antagonism to rice leaf spot bacteria and separate and apply |
| CN110669687A (en) * | 2019-09-06 | 2020-01-10 | 华南农业大学 | Application of bacillus proteolicus in preventing and treating pathogenic bacteria and diseases depending on quorum sensing signal molecules |
| CN114736828A (en) * | 2022-04-27 | 2022-07-12 | 西北农林科技大学深圳研究院 | Biocontrol strain and application thereof in plant disease control |
Non-Patent Citations (3)
| Title |
|---|
| 梁雪杰;郭殿豪;刘邮洲;唐永清;乔俊卿;杜艳;: "一株戴尔福特菌NF83-1的鉴定及评价", 植物保护学报, no. 02, pages 248 - 254 * |
| 盛存波;安德荣;鲁燕汶;全鑫;: "一株抗猕猴桃溃疡病的芽孢杆菌分离和筛选研究初报", 中国农学通报, no. 12, pages 346 - 348 * |
| 邵宝林;王成华;刘露希;张婧;庄启国;朱天辉;: "猕猴桃溃疡病生防芽孢杆菌B2的鉴定及应用", 中国农学通报, no. 26, pages 103 - 108 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116987648A (en) * | 2023-09-28 | 2023-11-03 | 西北农林科技大学深圳研究院 | Bacillus pumilus and application thereof in kiwi fruit canker prevention and treatment |
| CN116987648B (en) * | 2023-09-28 | 2023-11-28 | 西北农林科技大学深圳研究院 | Bacillus pumilus and application thereof in kiwi fruit canker prevention and treatment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116391726B (en) | 2023-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3428658B2 (en) | Antimicrobial microbial preparation, its production method and treatment method | |
| He et al. | Viability evaluation of alginate-encapsulated Pseudomonas putida Rs-198 under simulated salt-stress conditions and its effect on cotton growth | |
| CN101124915B (en) | Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method | |
| JP3536180B2 (en) | Compositions and methods for controlling plant diseases | |
| CN111172080A (en) | Bacillus belgii and application thereof | |
| PL175708B1 (en) | Application of streptomyces wyec 108 in fighting against plants pathogens | |
| CN110157638B (en) | Paenibacillus kribbensis YY-1 and application thereof | |
| CN116391726B (en) | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits | |
| CN112760248B (en) | Streptomyces lincolnensis capable of preventing and treating peach brown rot and application thereof | |
| CN109169712B (en) | Composite biological control agent and preparation method and application thereof | |
| CN116536207A (en) | Bacillus atrophaeus WLKYSY-4, biological microbial inoculum and application thereof | |
| CN115058358B (en) | Salt-tolerant bacillus and application thereof | |
| CN114350552B (en) | Bacillus pumilus and application thereof in control of medlar diseases | |
| CN106967656B (en) | Bacillus licheniformis and application thereof | |
| CN115595278B (en) | Ginseng disease-preventing growth-promoting bacterial fertilizer and application thereof | |
| CN111808785B (en) | Honghuadan endophytic Bacillus belgii and application thereof | |
| CN113545367B (en) | Photosynthetic bacteria live bacteria powder and preparation method and application thereof | |
| CN114916563B (en) | Bacillus amyloliquefaciens and application of synergist thereof | |
| CN116622537B (en) | Bacillus amyloliquefaciens ZA7-31, composite microbial inoculum, biocontrol preparation and application | |
| CN116240136B (en) | Leuconostoc mesenteroides WZ-44 for antagonizing erwinia amylovora and Asian erwinia amylovora and application thereof | |
| CN117384807B (en) | Bacillus mycoides HDMM 2 and application thereof | |
| FR2695929A1 (en) | Enriched microgranules, usable in combination with microbial inocula, their production and their application in agriculture. | |
| KR100227573B1 (en) | Controlling cyperus weeds with scochyta sp. ferm bp-5176 | |
| CN117821292A (en) | Bacillus amyloliquefaciens JK-2 and culture method and application thereof | |
| CN117757664A (en) | Biocontrol bacterium for preventing and treating grape root rot and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |