CN103980026A - Microbial fertilizer capable of promoting growth of rice seedling roots and preparation method thereof - Google Patents
Microbial fertilizer capable of promoting growth of rice seedling roots and preparation method thereof Download PDFInfo
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- CN103980026A CN103980026A CN201410214678.XA CN201410214678A CN103980026A CN 103980026 A CN103980026 A CN 103980026A CN 201410214678 A CN201410214678 A CN 201410214678A CN 103980026 A CN103980026 A CN 103980026A
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Abstract
The invention discloses a microbial fertilizer capable of promoting the growth of rice seedling roots and a preparation method thereof. The preparation method comprises the following steps: firstly, respectively fermenting klebsiella pneumoniae, bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, Heyu delftia and panebacillus polymyxa strains so as to obtain bacterial liquids, and then mixing the bacterial liquids so as to obtain a mixed fermentation bacterial liquid; and finally adding a mixed fertilizer, the mixed fermentation bacterial liquid and sterile water to organic materials, fermenting, then uniformly mixing the fertilizer obtained after fermentation with humic acid and grass carbon, and using a double-roll extrusion type pelletizer for preparing the microbial fertilizer. The microbial fertilizer disclosed by the invention is capable of promoting growth and development of rice seedling roots, resisting the influence of adverse environments on the growth of the rice seedling roots and achieving the purposes of strengthening roots and seedlings.
Description
Technical field
The present invention relates to agricultural fertilizer field, in particular a kind of microbial fertilizer that promotes rice seedling root growth and preparation method thereof.
Background technology
Rice Production belongs to labor-intensive industry, and along with Chinese labour force cost constantly increases, rice mechanization production, particularly mechanized planting, by a main trend of industry development after being.In order to be applicable to machine rice transplantation, paddy rice requires root system want many and become blanket shape when seedling, just be conducive to like this transportation and rice transplanting.Yet when the factors such as matrix, temperature that are sometimes subject in raising rice seedlings process affect, root growth is very poor, to machine rice transplantation, brings some problems.
Paddy rice is a little less than when seedling, easily there is rice shoot cold hardiness in early rice particularly, rice shoot is slim and frahile, bottom one to three leaf blade tip is often shrivelled shape, disease is more, the rotten kind of seedling rot etc., therefore the dedicated fertilizer of a kind of raising rice seedlings of needs, can promote seedling growth, can alleviate the generation of disease again, be conducive to again carrying out of mechanized planting.At present, although produce a lot of about the product of microorganism organic fertilizer, be directed to raising rice seedlings microbial fertilizer special less, particularly at short root, strengthen aspect root.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and a kind of microbial fertilizer that promotes rice seedling root growth and preparation method thereof is provided.
Technical scheme of the present invention is as follows:
Promote a preparation method for the microbial fertilizer of rice seedling root growth, its step is as follows:
(1) fermentation of Klebsiella pneumonia
The Klebsiella pneumonia bacterial strain list bacterium colony activating on LB solid medium is transferred to and is equipped with in the triangular flask that the volume of 10mL beef extract-peptone liquid nutrient medium is 50mL on Bechtop, shaking culture 20h under 30 ℃, 180rpm condition; Then be inoculated in and be equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, shaking culture 20h under 180rpm, 30 ℃ of conditions, be inoculated in afterwards in the mechanical agitation type fermentor tank that 20L fermention medium is housed, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, 30 ℃ of leavening temperatures, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration>=1.0 * 10
8cfu/mL;
(2) fermentation of Bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and Paenibacillus polymyxa
By the lichem bacillus strain list bacterium colony activating respectively on LB solid medium, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium or Paenibacillus polymyxa, on Bechtop, be transferred to and be equipped with in the triangular flask that the volume of 10mLLB liquid nutrient medium is 50mL, shaking culture 20h under 30 ℃, 180rpm condition; Then be inoculated in and be equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, shaking culture 20h under 180rpm, 30 ℃ of conditions, be inoculated in afterwards in the mechanical agitation type fermentor tank that 20L fermention medium is housed, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, 30 ℃ of leavening temperatures, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration>=1.0 * 10
8cfu/mL;
(3) preparation of mixed fermentation bacterium liquid
Klebsiella pneumonia, Bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium, Paenibacillus polymyxa that fermentation in (1) and (2) is obtained mix according to volume ratio 1.5:1:1:1:0.5, mix, make mixed fermentation bacterium liquid;
(4) preparation of microbial fertilizer
Rapeseed cake, soybean cake are mixed as organic materials according to weight 1:1, add azophoska according to purity nitrogen (urea): pure phosphorus (calcium superphosphate): pure potassium (potassium sulfate)=3:1:1 (weight ratio) mixes as mixed fertilizer, organic materials and mixed fertilizer are mixed, mix rear interpolation mixed fermentation bacterium liquid, add sterilized water, fermentation cylinder for fermentation 1 week simultaneously; After fermentation ends, fertilizer after fermentation and humic acid, the peat composed of rotten mosses are mixed, through pair roller extruding type tablets press, prepare microbial fertilizer, and detect living spores, organic matter and the moisture content of microbial fertilizer, make living spores content>=1.0 * 10
8cfu/g, organic content>=60%, moisture content is controlled between 8-10%, after the assay was approved packing.
The formula of described fermention medium is: soyflour 5g/L, Semen Maydis powder 5g/L, yeast extract 5g/L, brown sugar 50g/L, calcium carbonate 1g/L, MnSO
45mg/L, pH7.0-7.2, prepares with sterile distilled water.
Described preparation method, in step (4), every kg organic materials adds 12-15g mixed fertilizer; Every kg organic fertilizer adds 1ml mixed fermentation bacterium liquid; Every kg organic materials adds the sterilized water of 200ml.
Described preparation method, in step (4), after fermentation, the weight ratio of fertilizer and humic acid, the peat composed of rotten mosses is 10:1:2.
The microbial fertilizer of the promotion rice seedling root growth that described preparation method makes.
Microbial fertilizer of the present invention can significantly promote growing of rice seedling root system, strengthens the impact of opposing poor environment on Root Growth of Rice, allows rice seedling reach the object of strong root, strong seedling.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
The microbial strains of using in embodiment is purchased from Chinese industrial microbial strains preservation center and Chinese agriculture microbial strains preservation center.
Embodiment 1
1, microbial strains fermentation
(1) fermentation of Klebsiella pneumonia
The Klebsiella pneumonia bacterial strain list bacterium colony activating on LB solid medium is transferred to and is equipped with in the triangular flask that the volume of 10mL beef extract-peptone liquid nutrient medium is 50mL on Bechtop, shaking culture 20h under 30 ℃, 180rpm condition.Then be inoculated in and be equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, shaking culture 20h under 180rpm, 30 ℃ of conditions, be inoculated in afterwards in the mechanical agitation type fermentor tank that 20L fermention medium is housed, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, 30 ℃ of leavening temperatures, fermentation time 48h, pH6.8-7.2.The formula of wherein said fermention medium is: soyflour 5g/L, Semen Maydis powder 5g/L, yeast extract 5g/L, brown sugar 50g/L, calcium carbonate 1g/L, MnSO
45mg/L, pH7.0-7.2, prepares with sterile distilled water.After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration>=1.0 * 10
8cfu/mL.
(2) fermentation of Bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and Paenibacillus polymyxa
By the lichem bacillus strain list bacterium colony activating respectively on LB solid medium, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium or Paenibacillus polymyxa, on Bechtop, be transferred to and be equipped with in the triangular flask that the volume of 10mLLB liquid nutrient medium is 50mL, shaking culture 20h under 30 ℃, 180rpm condition.Then be inoculated in and be equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, shaking culture 20h under 180rpm, 30 ℃ of conditions, be inoculated in afterwards in the mechanical agitation type fermentor tank that 20L fermention medium (fermention medium in same 1) is housed, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, 30 ℃ of leavening temperatures, fermentation time 48h, pH6.8-7.2.After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration>=1.0 * 10
8cfu/mL.
The Klebsiella pneumonia that described fermentation is obtained, Bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium, Paenibacillus polymyxa mix according to volume ratio 1.5:1:1:1:0.5, mix, and make mixed fermentation bacterium liquid.
2, the preparation of microbial fertilizer
Rapeseed cake, soybean cake are mixed as organic materials according to weight 1:1, add azophoska according to purity nitrogen (urea): pure phosphorus (calcium superphosphate): pure potassium (potassium sulfate)=3:1:1 (weight ratio) mixes as mixed fertilizer, every kg organic materials adds 14g mixed fertilizer, mix rear interpolation mixed fermentation bacterium liquid, every kg organic fertilizer adds 1ml mixed fermentation bacterium liquid, simultaneously every kg organic materials added the sterilized water of 200ml, fermentation cylinder for fermentation 1 week.After fermentation ends, fertilizer after fermentation and humic acid, the peat composed of rotten mosses are mixed according to weight ratio 10:1:2, through pair roller extruding type tablets press, prepare microbial fertilizer, and detect living spores, organic matter and the moisture content of microbial fertilizer, make living spores content>=1.0 * 10
8cfu/g, organic content>=60%, moisture content is controlled between 8-10%, after the assay was approved packing.
Embodiment 2
In greenhouse, base, Fuyang, China Paddy Rice Inst Zhejiang Province, carry out paddy rice simultaneous test, 3 processing, CK are established in test: chemical fertilizer contrast, is the mixed fertilizer in microbial fertilizer; The mixture that A: use organic materials--rapeseed cake, soybean cake mix according to weight ratio 1:1; B: the microbial fertilizer of using embodiment 1 preparation.Microbial fertilizer consumption: add 500g, Routine Management by the every dish of common seedling dish (60 * 25) during raising rice seedlings.After 20 days, observe rice seedling growing state, confirm whether growth-promoting bacterial strain has the effect that promotes Root Growth of Rice.Through observing, find, along with the prolongation of rice seedling growth time, use the microbial fertilizer of embodiment 1 preparation and process (B), its growing way is much better than to use the rice seedling (table 1) of chemical fertilizer (CK) and organic materials (A).
The impact of the microbial fertilizer that table 1 is used embodiment 1 preparation on rice root and overground part growth
With CK comparison, to use after the microbial fertilizer of embodiment 1 preparation, rice root length, weight of root system, overground part dry weight have increased respectively 46%, 41% and 33%.With A comparison, to use after the microbial fertilizer of embodiment 1 preparation, rice root length, weight of root system, overground part dry weight have increased respectively 21%, 24% and 21%.The microbial fertilizer of promotion rice seedling root growth of the present invention is suitable for the paddy machinery rice transplanting seedling cultivation demands such as China double cropping of rice early rice, the single harvest rice, after use, can improve the resistance of rice seedling, healthy and strong rice shoot, rice shoot disease obviously reduces, root length significantly increases, growth evenly, reaches the requirement of transplanting.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (5)
1. a preparation method who promotes the microbial fertilizer of rice seedling root growth, is characterized in that, its step is as follows:
(1) fermentation of Klebsiella pneumonia
The Klebsiella pneumonia bacterial strain list bacterium colony activating on LB solid medium is transferred to and is equipped with in the triangular flask that the volume of 10mL beef extract-peptone liquid nutrient medium is 50mL on Bechtop, shaking culture 20h under 30 ℃, 180rpm condition; Then be inoculated in and be equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, shaking culture 20h under 180rpm, 30 ℃ of conditions, be inoculated in afterwards in the mechanical agitation type fermentor tank that 20L fermention medium is housed, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, 30 ℃ of leavening temperatures, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration>=1.0 * 10
8cfu/mL;
(2) fermentation of Bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and Paenibacillus polymyxa
By the lichem bacillus strain list bacterium colony activating respectively on LB solid medium, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium or Paenibacillus polymyxa, on Bechtop, be transferred to and be equipped with in the triangular flask that the volume of 10mLLB liquid nutrient medium is 50mL, shaking culture 20h under 30 ℃, 180rpm condition; Then be inoculated in and be equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, shaking culture 20h under 180rpm, 30 ℃ of conditions, be inoculated in afterwards in the mechanical agitation type fermentor tank that 20L fermention medium is housed, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, 30 ℃ of leavening temperatures, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration>=1.0 * 10
8cfu/mL;
(3) preparation of mixed fermentation bacterium liquid
Klebsiella pneumonia, Bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium, Paenibacillus polymyxa that fermentation in (1) and (2) is obtained mix according to volume ratio 1.5:1:1:1:0.5, mix, make mixed fermentation bacterium liquid;
(4) preparation of microbial fertilizer
Rapeseed cake, soybean cake are mixed as organic materials according to weight 1:1, add azophoska according to purity nitrogen: pure phosphorus: the weight ratio of pure potassium is that 3:1:1 mixes as mixed fertilizer, organic materials and mixed fertilizer are mixed, mix rear interpolation mixed fermentation bacterium liquid, add sterilized water, fermentation cylinder for fermentation 1 week simultaneously; After fermentation ends, fertilizer after fermentation and humic acid, the peat composed of rotten mosses are mixed, through pair roller extruding type tablets press, prepare microbial fertilizer, and detect living spores, organic matter and the moisture content of microbial fertilizer, make living spores content>=1.0 * 10
8cfu/g, organic content>=60%, moisture content is controlled between 8-10%, after the assay was approved packing.
2. preparation method according to claim 1, is characterized in that, the formula of described fermention medium is: soyflour 5g/L, Semen Maydis powder 5g/L, yeast extract 5g/L, brown sugar 50g/L, calcium carbonate 1g/L, MnSO
45mg/L, pH7.0-7.2, prepares with sterile distilled water.
3. preparation method according to claim 1, is characterized in that, in step (4), every kg organic materials adds 12-15g mixed fertilizer; Every kg organic fertilizer adds 1ml mixed fermentation bacterium liquid; Every kg organic materials adds the sterilized water of 200ml.
4. preparation method according to claim 1, is characterized in that, in step (4), after fermentation, the weight ratio of fertilizer and humic acid, the peat composed of rotten mosses is 10:1:2.
5. the microbial fertilizer of the promotion rice seedling root growth making according to the preparation method described in claim 1～4.
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Cited By (7)
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| CN106336320A (en) * | 2016-08-16 | 2017-01-18 | 朱兰英 | Preparation method of fertilizer for improving harmful phytophagous nematode in soil |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
| CN107520248A (en) * | 2017-09-12 | 2017-12-29 | 贵州省草业研究所 | A kind of method that crops repair farmland mercury contaminated soil |
| CN112898095A (en) * | 2021-03-22 | 2021-06-04 | 广西农垦西江乳业有限公司 | Preparation method of cow dung organic fertilizer |
| CN113307661A (en) * | 2021-04-20 | 2021-08-27 | 河南省农业科学院植物营养与资源环境研究所 | Microbial fertilizer for field and preparation method thereof |
| CN114467646A (en) * | 2022-01-23 | 2022-05-13 | 黑龙江八一农垦大学 | Bacterium and substrate for seedling culture and seedling culture method |
| CN116391726A (en) * | 2023-05-26 | 2023-07-07 | 西北农林科技大学深圳研究院 | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits |
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