CN103980026B - A kind of microbial manure that promotes rice seedling root growth and preparation method thereof - Google Patents
A kind of microbial manure that promotes rice seedling root growth and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of microbial manure that promotes rice seedling root growth and preparation method thereof. First the present invention is fermented respectively and is made bacterium liquid by Friedlander's bacillus, bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and Paenibacillus polymyxa bacterial classification, then its mixing is made to mixed culture fermentation bacterium liquid; Finally in organic materials, add mixed fertilizer, mixed culture fermentation bacterium liquid and sterilized water, fermentation, after fermentation ends, mixes fertilizer after fermentation and humic acid, the peat composed of rotten mosses, prepares microbial manure through pair roller squash type comminutor. Microbial manure of the present invention can significantly promote growing of rice seedling root system, strengthens the impact of opposing poor environment on Root Growth of Rice, allows rice seedling reach the object of strong root, strong seedling.
Description
Technical field
The present invention relates to agricultural fertilizer field, in particular a kind of microbial manure that promotes rice seedling root growthAnd preparation method thereof.
Background technology
Rice Production belongs to labor-intensive production, and along with Chinese labour force cost constantly increases, rice mechanization is produced,Particularly mechanized planting, by a main trend of industry development after being. In order to be applicable to machine rice transplantation, paddy rice is educatingWhen seedling, require root system want many and become blanket shape, be so just conducive to transport and rice transplanting. But in raising rice seedlings process sometimesWhen the factor such as matrix, temperature that is subject to affects, root growth is very poor, brings some problems to machine rice transplantation.
Paddy rice is a little less than in the time of seedling, easily there is rice shoot cold hardiness in early rice particularly, and rice shoot is slim and frahile, and bottom one to three leaf blade tip is normalBe shrivelled shape, disease is more, and therefore the rotten kind of seedling rot etc. need a kind of dedicated fertilizer of raising rice seedlings, can promote rice shootGrowth, the generation that can alleviate again disease, is conducive to again carrying out of mechanized planting. At present, although produce upper about micro-lifeThe product of organic fertilizer material is a lot, but be directed to raising rice seedlings microbial fertilizer special less, particularly short root,Strong root aspect.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and a kind of promotion rice seedling root system is providedMicrobial manure of growth and preparation method thereof.
Technical scheme of the present invention is as follows:
Promote a preparation method for the microbial manure of rice seedling root growth, its step is as follows:
(1) fermentation of Friedlander's bacillus
The Friedlander's bacillus bacterial strain list bacterium colony activating on LB solid medium is transferred to dress on superclean benchIn the triangular flask that the volume that has 10mL beef extract-peptone fluid nutrient medium is 50mL, in 30 DEG C, 180rpm conditionLower shaken cultivation 20h; Then being inoculated in the volume that 400mL beef-protein medium is housed is 1000mLTriangular flask in, shaken cultivation 20h under 180rpm, 30 DEG C of conditions, is inoculated in afterwards 20L fermented and cultured is housedIn the mechanical agitation type fermentation tank of base, fermentation condition is set as: dissolved oxygen 100%, mixing speed 350rpm, fermentation temperature30 DEG C, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living spores concentration, guarantees the bud of livingSpore concentration >=1.0 × 108cfu/mL；
(2) bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and how sticky class brood cellThe fermentation of bacillus
By the lichem bacillus strain list bacterium colony activating respectively on LB solid medium, bacillus amyloliquefaciens plantSubspecies, He Yu field Dell Ford bacterium or Paenibacillus polymyxa are transferred to 10mLLB liquid are housed on superclean benchThe volume of body culture medium is in the triangular flask of 50mL, shaken cultivation 20h under 30 DEG C, 180rpm condition; Then willIt is inoculated in and is equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, 180rpm, 30Shaken cultivation 20h under DEG C condition, is inoculated in the mechanical agitation type fermentation tank that 20L fermentation medium is housed afterwards,Fermentation condition is set as: dissolved oxygen 100%, mixing speed 350rpm, 30 DEG C of fermentation temperatures, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration >=1.0 × 108cfu/mL；
(3) preparation of mixed culture fermentation bacterium liquid
Friedlander's bacillus, bacillus licheniformis, bacillus amyloliquefaciens that fermentation in (1) and (2) is obtained are plantedThing subspecies, He Yu field Dell Ford bacterium, Paenibacillus polymyxa mix according to volume ratio 1.5:1:1:1:0.5, mix,Make mixed culture fermentation bacterium liquid;
(4) preparation of microbial manure
Rape cake, soybean cake are mixed as organic materials according to weight 1:1, add azophoska according to purity nitrogen (urea):Pure phosphorus (calcium superphosphate): pure potassium (potassium sulfate)=3:1:1 (weight ratio) mixes as mixed fertilizer, by organic materials andMixed fertilizer mixed, and mixes rear interpolation mixed culture fermentation bacterium liquid, adds sterilized water simultaneously, fermentation cylinder for fermentation 1 week; Send outAfter ferment finishes, fertilizer after fermentation and humic acid, the peat composed of rotten mosses are mixed, prepare microbial manure through pair roller squash type comminutor,And detect living spores, organic matter and the moisture of microbial manure, make living spores content >=1.0 × 108Cfu/g, organicMatter content >=60%, moisture is controlled between 8-10%, after the assay was approved packaging.
The formula of described fermentation medium is: soy meal 5g/L, corn flour 5g/L, yeast extract 5g/L, brown sugar50g/L, calcium carbonate 1g/L, MnSO45mg/L, pH7.0-7.2, prepares with sterile distilled water.
Described preparation method, in step (4), every kg organic materials adds 12-15g mixed fertilizer; Every kg fertilizerMaterial adds 1ml mixed culture fermentation bacterium liquid; Every kg organic materials adds the sterilized water of 200ml.
Described preparation method, in step (4), after fermentation, the weight ratio of fertilizer and humic acid, the peat composed of rotten mosses is 10:1:2.
The microbial manure of the promotion rice seedling root growth that described preparation method makes.
Microbial manure of the present invention can significantly promote growing of rice seedling root system, strengthens opposing poor environment to waterThe impact of rice root growth, allows rice seedling reach the object of strong root, strong seedling.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
The microorganism fungus kind of using in embodiment is purchased from Chinese industrial microorganism fungus kind preservation center and Chinese agriculture microorganismDSMZ.
Embodiment 1
1, microorganism fungus kind fermentation
(1) fermentation of Friedlander's bacillus
The Friedlander's bacillus bacterial strain list bacterium colony activating on LB solid medium is transferred to dress on superclean benchIn the triangular flask that the volume that has 10mL beef extract-peptone fluid nutrient medium is 50mL, in 30 DEG C, 180rpm conditionLower shaken cultivation 20h. Then being inoculated in the volume that 400mL beef-protein medium is housed is 1000mLTriangular flask in, shaken cultivation 20h under 180rpm, 30 DEG C of conditions, is inoculated in afterwards 20L fermented and cultured is housedIn the mechanical agitation type fermentation tank of base, fermentation condition is set as: dissolved oxygen 100%, mixing speed 350rpm, fermentation temperature30 DEG C, fermentation time 48h, pH6.8-7.2. The formula of wherein said fermentation medium is: soy meal 5g/L, cornPowder 5g/L, yeast extract 5g/L, brown sugar 50g/L, calcium carbonate 1g/L, MnSO45mg/L，pH7.0-7.2，Prepare with sterile distilled water. After fermentation is reached home, sampling detects living spores concentration, guarantees living spores concentration >=1.0 × 108cfu/mL。
(2) bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and how sticky class brood cellThe fermentation of bacillus
By the lichem bacillus strain list bacterium colony activating respectively on LB solid medium, bacillus amyloliquefaciens plantSubspecies, He Yu field Dell Ford bacterium or Paenibacillus polymyxa are transferred to 10mLLB liquid are housed on superclean benchThe volume of body culture medium is in the triangular flask of 50mL, shaken cultivation 20h under 30 DEG C, 180rpm condition. Then willIt is inoculated in and is equipped with in the triangular flask that the volume of 400mL beef-protein medium is 1000mL, 180rpm, 30Shaken cultivation 20h under DEG C condition, is inoculated in afterwards 20L fermentation medium (with fermentation medium in 1) is housedMechanical agitation type fermentation tank in, fermentation condition is set as: dissolved oxygen 100%, mixing speed 350rpm, fermentation temperature30 DEG C, fermentation time 48h, pH6.8-7.2. After fermentation is reached home, sampling detects living spores concentration, guarantees the bud of livingSpore concentration >=1.0 × 108cfu/mL。
Friedlander's bacillus, bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, crane that described fermentation is obtainedYu Tian Dell Ford bacterium, Paenibacillus polymyxa mix according to volume ratio 1.5:1:1:1:0.5, mix, and make mixingZymocyte liquid.
2, the preparation of microbial manure
Rape cake, soybean cake are mixed as organic materials according to weight 1:1, add azophoska according to purity nitrogen (urea):Pure phosphorus (calcium superphosphate): pure potassium (potassium sulfate)=3:1:1 (weight ratio) mixes as mixed fertilizer, every kg organic matterMaterial adds 14g mixed fertilizer, mixes rear interpolation mixed culture fermentation bacterium liquid, and every kg organic fertilizer adds 1ml mixed culture fermentation bacterium liquid,Simultaneously every kg organic materials added the sterilized water of 200ml, fermentation cylinder for fermentation 1 week. After fermentation ends, will fermentRear fertilizer and humic acid, the peat composed of rotten mosses mix according to weight ratio 10:1:2, prepare microbial manure through pair roller squash type comminutor,And detect living spores, organic matter and the moisture of microbial manure, make living spores content >=1.0 × 108Cfu/g, organicMatter content >=60%, moisture is controlled between 8-10%, after the assay was approved packaging.
Embodiment 2
Carry out paddy rice contrast test in greenhouse, base, Fuyang, Zhejiang Province of China Paddy Rice Inst, 3 processing, CK are established in test:Chemical fertilizer contrast, is the mixed fertilizer in microbial manure; A: use organic materials--rape cake, soybean cake are according to heavyThe mixture that amount mixes than 1:1; B: use microbial manure prepared by embodiment 1. Microbial manure consumption: paddy riceWhen seedling, add 500g, Routine Management by the every dish of common seedling dish (60 × 25). After 20 days, observe rice seedling growthSituation, confirms whether growth-promoting bacterial strain has the effect that promotes Root Growth of Rice. Find through observing, along with rice seedling growthThe prolongation of time, uses microbial manure processing (B) prepared by embodiment 1, and its growing way is much better than to use chemical fertilizer (CK)And the rice seedling (table 1) of organic materials (A).
Table 1 is used microbial manure prepared by embodiment 1 impact on rice root and overground part growth
With CK comparison, use after microbial manure prepared by embodiment 1 rice root length, weight of root system, on the groundPortion's dry weight has increased respectively 46%, 41% and 33%. With A comparison, use after microbial manure prepared by embodiment 1,Rice root length, weight of root system, overground part dry weight have increased respectively 21%, 24% and 21%. Promotion of the present inventionThe microbial manure of rice seedling root growth is suitable for the paddy machinery rice transplanting rice shoot trainings such as China double cropping of rice early rice, the single harvest riceEducate demand, can improve the resistance of rice seedling, healthy and strong rice shoot after use, rice shoot disease obviously reduces, and root length is aobviousWork increases, and growth evenly, reaches the requirement of transplanting.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert,And all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (5)
1. a preparation method who promotes the microbial manure of rice seedling root growth, is characterized in that,Its step is as follows:
(1) fermentation of Friedlander's bacillus
The Friedlander's bacillus bacterial strain list bacterium colony activating on LB solid medium is turned on superclean benchReceive and be equipped with in the triangular flask that the volume of 10mL beef extract-peptone fluid nutrient medium is 50mL, in 30 DEG C,Shaken cultivation 20h under 180rpm condition; Then be inoculated in the cultivation of 400mL beef extract-peptone is housedThe volume of base is in the triangular flask of 1000mL, and shaken cultivation 20h under 180rpm, 30 DEG C of conditions afterwards willIt is inoculated in the mechanical agitation type fermentation tank that 20L fermentation medium is housed, and fermentation condition is set as: dissolved oxygen100%, mixing speed 350rpm, 30 DEG C of fermentation temperatures, fermentation time 48h, pH6.8-7.2; Fermentation is arrivedReach after terminal, sampling detects living spores concentration, guarantees living spores concentration >=1.0 × 108cfu/mL；
(2) bacillus licheniformis, bacillus amyloliquefaciens plant subspecies, He Yu field Dell Ford bacterium and how stickyThe fermentation of class bacillus
By the lichem bacillus strain list bacterium colony activating respectively, separate starch gemma bar on LB solid mediumBacterium plant subspecies, He Yu field Dell Ford bacterium or Paenibacillus polymyxa are transferred to and are equipped with on superclean benchThe volume of 10mLLB fluid nutrient medium is in the triangular flask of 50mL, under 30 DEG C, 180rpm condition, vibratesCultivate 20h; Then being inoculated in the volume that 400mL beef-protein medium is housed is 1000mLTriangular flask in, shaken cultivation 20h under 180rpm, 30 DEG C of conditions, is inoculated in afterwards and 20L is housed sends outIn the mechanical agitation type fermentation tank of ferment culture medium, fermentation condition is set as: dissolved oxygen 100%, and mixing speed 350rpm,30 DEG C of fermentation temperatures, fermentation time 48h, pH6.8-7.2; After fermentation is reached home, sampling detects living sporesConcentration, guarantees living spores concentration >=1.0 × 108cfu/mL；
(3) preparation of mixed culture fermentation bacterium liquid
Friedlander's bacillus, bacillus licheniformis, solution starch gemma that fermentation in (1) and (2) is obtainedBacillus plant subspecies, He Yu field Dell Ford bacterium, Paenibacillus polymyxa mix according to volume ratio 1.5:1:1:1:0.5Close, mix, make mixed culture fermentation bacterium liquid;
(4) preparation of microbial manure
Rape cake, soybean cake are mixed as organic materials according to weight 1:1, add azophoska according to purity nitrogen:Pure phosphorus: the weight ratio of pure potassium is that 3:1:1 mixes as mixed fertilizer, mixes organic materials and mixed fertilizer,Mix rear interpolation mixed culture fermentation bacterium liquid, add sterilized water simultaneously, fermentation cylinder for fermentation 1 week; After fermentation ends,Fertilizer after fermentation and humic acid, the peat composed of rotten mosses are mixed, prepare microbial manure through pair roller squash type comminutor, and inspectionLiving spores, organic matter and the moisture of micrometer bio-feritlizer, make living spores content >=1.0 × 108Cfu/g, hasMachine matter content >=60%, moisture is controlled between 8-10%, after the assay was approved packaging.
2. preparation method according to claim 1, is characterized in that, the joining of described fermentation mediumFang Wei: soy meal 5g/L, corn flour 5g/L, yeast extract 5g/L, brown sugar 50g/L, calcium carbonate 1g/L,MnSO45mg/L, pH7.0-7.2, prepares with sterile distilled water.
3. preparation method according to claim 1, is characterized in that, in step (4), and every kgOrganic materials adds 12-15g mixed fertilizer; Every kg organic materials adds 1ml mixed culture fermentation bacterium liquid; Every kg is organicMaterial adds the sterilized water of 200ml.
4. preparation method according to claim 1, is characterized in that, in step (4), after fermentationThe weight ratio of fertilizer and humic acid, the peat composed of rotten mosses is 10:1:2.
5. the promotion rice seedling root growth making according to the preparation method described in claim 1～4Microbial manure.
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| CN107382622B (en) * | 2017-08-17 | 2021-08-10 | 山东天鹿生物科技有限公司 | Microbial agent containing bacillus polymyxa and application thereof |
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| CN113307661A (en) * | 2021-04-20 | 2021-08-27 | 河南省农业科学院植物营养与资源环境研究所 | Microbial fertilizer for field and preparation method thereof |
| CN114467646B (en) * | 2022-01-23 | 2023-03-03 | 黑龙江八一农垦大学 | Bacterium and substrate for seedling culture and seedling culture method |
| CN116391726B (en) * | 2023-05-26 | 2023-09-05 | 西北农林科技大学深圳研究院 | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits |
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