CN106083490A - Utilize biological organic fertilizer prepared by glutamic acid fermentation discarded object - Google Patents
Utilize biological organic fertilizer prepared by glutamic acid fermentation discarded object Download PDFInfo
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- CN106083490A CN106083490A CN201610400307.XA CN201610400307A CN106083490A CN 106083490 A CN106083490 A CN 106083490A CN 201610400307 A CN201610400307 A CN 201610400307A CN 106083490 A CN106083490 A CN 106083490A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/90—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
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- Organic Chemistry (AREA)
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- Pest Control & Pesticides (AREA)
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Abstract
The invention belongs to biofermentation industry fermentation wastes process field, disclose the biological organic fertilizer utilizing glutamic acid fermentation discarded object to prepare, it is prepared in accordance with the following steps: step 1) step 1) glutamic acid fermentation discarded object filters, step 2) prepare nitrogen source, step 3) prepares diazotroph preparation, step 4) prepares weeding biologic product, and step 5) prepares soil conditioner, and step 6) mixes and is dried.Biological organic fertilizer effect of the present invention is lasting, and flora and soil conditioner serve fixed nitrogen in soil and protect the effects such as potassium, improve soil quality, it is achieved that sustainable development.
Description
Technical field
The invention belongs to biofermentation industry fermentation wastes process field, particularly relate to glutamic acid fermentation discarded object
The biological organic fertilizer of preparation.
Background technology
Glutamic acid, is a kind of acidic amino acid.Intramolecular contains two carboxyls, and chemical name is alpha-amido glutaric acid.Paddy ammonia
Acid is inner Suo Xun discovery in 1856, is clear crystal, has delicate flavour, be present in a large number in grain protein, content in animal brain
Also more.Account for critical role during glutamic acid protein metabolism in vivo, participate in animal, plant and microorganism
Many important chemical reactions.Sodium glutamate is commonly called as monosodium glutamate, is important tasty agents, has humidification to fragrance.Sodium glutamate
It is widely used in food flavor, both can be used alone, can be used in combination with other amino acid etc. again.For in food, there is flavouring effect.
With the development of amino acid fermentation technology, China has become as the production and consumption big country of monosodium glutamate, but taste
In essence production process, the wastewater flow rate that discharged is big, and the mother liquor that fermented gournet powder liquid discharges after extracting glutamic acid through waiting electricity has CODCr
Height, BOD5 are high, thalline content is high, sulfate radical (using instead before sulfuric acid adjusts pH is chlorion) content height, ammonia-nitrogen content are high and pH value
(1.5-3.2) low " five high one low " feature.It is the industrial wastewater that a kind of difficulty of governance is very big.Owing to can not effectively administer taste
Essence waste water, many Gourmet Powder Factories are put into the row of whole nation major polluting sources unit, and the improvement of gourmet powder waste water has become as restriction monosodium glutamate
The great difficult problem of manufacturing enterprise's development.The a large amount of thalline containing in waste water, it is a kind of single cell protein, containing abundant albumen
Matter.
The development of China's fertilizer and application history, be to be Main Stage from farm manure to using inorganic fertilizer, because farm manure is dirty
Dye source is many, and freight volume is big, operationally wastes time and energy and effect is not obvious especially；And inorganic fertilizer composition is single, utilization rate
Relatively low, and often cause soil compaction and with soil erosion polluted source, influence ecological environment.At present, market is adopted more
With the inorganic composite fertilizer etc. of Nitrogen-and Phosphorus-containing potassium, the fertilizer efficiency of this composite fertilizer is comprehensive, but it is not lasting to there is also fertilizer efficiency, and easy loss etc. lacks
Fall into；The more important thing is, the price of current composite fertilizer is higher, brings bigger burden to peasant, how to reduce fertilizer cost, improves
Farmers' income is the agricultural modernization technical issues that need to address." one utilizes glutamic acid to send out to patented technology before applicant
Bio-bacterial manure prepared by ferment discarded object " uses the mode of hydrolysis mycoprotein to prepare amino acid chelate fertilizer, but the method is suitable for
Preparing chelating fertilizer, microbial inoculum therein only serves booster action, and is not pure biological organic fertilizer, and effect is single.
Content of the invention
In order to overcome the defect of prior art, the invention provides the biology utilizing glutamic acid fermentation discarded object to prepare organic
Fertilizer, this biological organic fertilizer is effectively utilized glutamic acid fermentation discarded object, reduces fertilizer production cost, it is achieved that turn waste into wealth, and
And fertilizer efficiency is lasting, has both weeding function.
For achieving the above object, the present invention is by the following technical solutions:
Utilizing biological organic fertilizer prepared by glutamic acid fermentation discarded object, it is prepared in accordance with the following steps: step 1) glutamic acid
Fermentation wastes filters, step 2) prepare nitrogen source, step 3) prepares diazotroph preparation, and step 4) prepares weeding biologic product,
Step 5) prepares soil conditioner, and step 6) mixes and is dried.
Specifically, it is prepared in accordance with the following steps:
Step 1) glutamic acid fermentation discarded object filters: the glutamic acid fermentation discarded object producing fermentable glutamic acid filters,
Collect mycoprotein and waste water；
Step 2) prepare nitrogen source: drying mycoprotein, pulverizer is ground into powder, is subsequently placed in retort, adds
The hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
Should use in ammoniacal liquor after terminating and remaining hydrochloric acid, the pH of control solution is 6.8-7.2；
Step 3) prepares diazotroph preparation: pulverizes wheat stalk and obtains wheat stalk powder, then with wheat bran, rapeseed dregs and
Fishbone dust mixing and stirring obtains compound, adds the waste water of the step 1) accounting for compound twice quality, soaks 12 hours, then
Boil 15min, be cooled to room temperature, according to 10%(v/v) the compound fixed nitrogen bacterium solution of inoculum concentration inoculation, at the bar that temperature is 28-30 DEG C
Part ferments 36 hours, obtains diazotroph preparation；Wherein, the mass ratio of wheat stalk powder, wheat bran, rapeseed dregs and fishbone dust is
5-9:4-7:3-4:2-3；Described compound fixed nitrogen bacterium solution is prepared in accordance with the following steps: by bacillus megaterium, arthrobacterium, circle
Brown nitrogen-fixing bacteria, bacillus polymyxa cultivate respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume of 5:4:3:3
Than mixing, to obtain final product；
Step 4) prepares weeding biologic product: wheat stalk is ground into wheat stalk powder, adds step 2 to) solution in, stir
Mix uniformly, stands 12 hours, then interpolation dregs of beans, vinasse, oyster shell whiting, potassium dihydrogen phosphate and glucose successively, 500 revs/min
Clock stirs 3 minutes, then the programming rate with 5 DEG C/min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, press
According to 10%(v/v) inoculum concentration inoculation compound herbicide bacterium solution, control at pH6.8-7.2, temperature be 28-30 DEG C condition fermentation 36
Hour, obtain weeding biologic product；Wherein, wheat stalk powder, step 2) solution, dregs of beans, vinasse, oyster shell whiting, biphosphate
The mass ratio of potassium and glucose is 8-12:70-90:10-12:5-6:5-6:3-4:1-2；
Described compound herbicide bacterium solution is prepared in accordance with the following steps: cultivate trichoderma aureoviride, candidiasis to concentration respectively
It is 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:1, to obtain final product；
Step 5) prepares soil conditioner: stir humic acid, peat soil and attapulgite according to the mass ratio mixing of 2:1:1
Mix uniformly, then grind, cross 200 mesh sieves and i.e. obtain soil conditioner；
Step 6) mixes and is dried: by diazotroph preparation, weeding biologic product, soil conditioner according to 8-12:6-9:2-3's
Mass ratio mixes, and stirs, and then freeze-drying is prepared as dry powder, to obtain final product.
Preferably, described bacillus megaterium is CGMCC No.3770, and described arthrobacterium is CGMCC No.7779, described
Azotobacter chroococcum is ATCC No.4412, and bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC No.22023.
Above-mentioned bacterial strains all can be bought from preservation center or other commercial channel and obtain.
The beneficial effect that the present invention obtains includes the following aspects:
Fermentation wastes is applied all in fertilizer preparation by the present invention, it is to avoid be evaporated to waste liquid concentrating and subtracting at dirt
Reason, decreases energy waste, improves utilization rate, and be prepared for fertilizer, kill two birds with one stone；The discarded bacterium of direct hydrolysis of the present invention
Body protein, as fermentation raw material, provides abundant ammonium chloride and amino acid nitrogen source, can not only be used for fermentable nutriment, also
Can be as Fertilizer application；Bacterial strain compatibility is reasonable, uses different biologic product different fermentations processing mode so that different strains is in
Optimal fermentation condition, it is to avoid part bacterial strain can not be survived in nutrient solution prepared by high-concentration waste water；The present invention is by effectively
Utilizing the agricultural waste material of naturally occurring, in conjunction with microbial technique so that fertilizer cost is cheap, fertilizer efficiency is more lasting；Soil
Conditioner, as nutriment and carrier, can possess water conservation fertilizer conservation function to nutrients release adjusting function, improves soil, and to soil
The multiple middle and trace element of middle release, increases soil fertility, moreover it is possible to use as microbe carrier, kills two birds with one stone；Microbial inoculum of the present invention
Through screening and reasonable compatibility so that fertilizer possesses fixed nitrogen water conservation, and increasing crop yield increases income, and is also equipped with certain weeding function,
Environment friendly and pollution-free.
Detailed description of the invention
In order to make the present invention realize technological means, creation characteristic, reach purpose and be easy to understand with effect, tie below
Close specific embodiment, the present invention is expanded on further.
Embodiment 1
Utilizing biological organic fertilizer prepared by glutamic acid fermentation discarded object, it is prepared in accordance with the following steps: step 1) glutamic acid
Fermentation wastes filters, step 2) prepare nitrogen source, step 3) prepares diazotroph preparation, and step 4) prepares weeding biologic product,
Step 5) prepares soil conditioner, and step 6) mixes and is dried.
Specifically, it is prepared in accordance with the following steps:
Step 1) glutamic acid fermentation discarded object filters: the glutamic acid fermentation discarded object producing fermentable glutamic acid filters,
Collect mycoprotein and waste water；
Step 2) prepare nitrogen source: drying mycoprotein, pulverizer is ground into powder, is subsequently placed in retort, adds
The hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
Should use in ammoniacal liquor after terminating and remaining hydrochloric acid, the pH of control solution is 6.8-7.2；
Step 3) prepares diazotroph preparation: pulverizes wheat stalk and obtains wheat stalk powder, then with wheat bran, rapeseed dregs and
Fishbone dust mixing and stirring obtains compound, adds the waste water of the step 1) accounting for compound twice quality, soaks 12 hours, then
Boil 15min, be cooled to room temperature, according to 10%(v/v) the compound fixed nitrogen bacterium solution of inoculum concentration inoculation, at the bar that temperature is 28-30 DEG C
Part ferments 36 hours, obtains diazotroph preparation；Wherein, the mass ratio of wheat stalk powder, wheat bran, rapeseed dregs and fishbone dust is
5:4:3:2；Described compound fixed nitrogen bacterium solution is prepared in accordance with the following steps: by bacillus megaterium, arthrobacterium, the brown fixed nitrogen of circle
Bacterium, bacillus polymyxa cultivate respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 5:4:3:3,
Obtain；
Step 4) prepares weeding biologic product: wheat stalk is ground into wheat stalk powder, adds step 2 to) solution in, stir
Mix uniformly, stands 12 hours, then interpolation dregs of beans, vinasse, oyster shell whiting, potassium dihydrogen phosphate and glucose successively, 500 revs/min
Clock stirs 3 minutes, then the programming rate with 5 DEG C/min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, press
According to 10%(v/v) inoculum concentration inoculation compound herbicide bacterium solution, control at pH6.8-7.2, temperature be 28-30 DEG C condition fermentation 36
Hour, obtain weeding biologic product；Wherein, wheat stalk powder, step 2) solution, dregs of beans, vinasse, oyster shell whiting, biphosphate
The mass ratio of potassium and glucose is 8:70:10:5:5:3:1；
Described compound herbicide bacterium solution is prepared in accordance with the following steps: cultivate trichoderma aureoviride, candidiasis to concentration respectively
It is 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:1, to obtain final product；
Step 5) prepares soil conditioner: stir humic acid, peat soil and attapulgite according to the mass ratio mixing of 2:1:1
Mix uniformly, then grind, cross 200 mesh sieves and i.e. obtain soil conditioner；
Step 6) mixes and is dried: by diazotroph preparation, weeding biologic product, soil conditioner according to the mass ratio of 8:6:2
Mixing, stirs, and then freeze-drying is prepared as dry powder, to obtain final product.
Preferably, described bacillus megaterium is CGMCC No.3770, and described arthrobacterium is CGMCC No.7779, described
Azotobacter chroococcum is ATCC No.4412, and bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC No.22023.
Embodiment 2
Utilizing biological organic fertilizer prepared by glutamic acid fermentation discarded object, it is prepared in accordance with the following steps: step 1) glutamic acid
Fermentation wastes filters, step 2) prepare nitrogen source, step 3) prepares diazotroph preparation, and step 4) prepares weeding biologic product,
Step 5) prepares soil conditioner, and step 6) mixes and is dried.
Specifically, it is prepared in accordance with the following steps:
Step 1) glutamic acid fermentation discarded object filters: the glutamic acid fermentation discarded object producing fermentable glutamic acid filters,
Collect mycoprotein and waste water；
Step 2) prepare nitrogen source: drying mycoprotein, pulverizer is ground into powder, is subsequently placed in retort, adds
The hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
Should use in ammoniacal liquor after terminating and remaining hydrochloric acid, the pH of control solution is 6.8-7.2；
Step 3) prepares diazotroph preparation: pulverizes wheat stalk and obtains wheat stalk powder, then with wheat bran, rapeseed dregs and
Fishbone dust mixing and stirring obtains compound, adds the waste water of the step 1) accounting for compound twice quality, soaks 12 hours, then
Boil 15min, be cooled to room temperature, according to 10%(v/v) the compound fixed nitrogen bacterium solution of inoculum concentration inoculation, at the bar that temperature is 28-30 DEG C
Part ferments 36 hours, obtains diazotroph preparation；Wherein, the mass ratio of wheat stalk powder, wheat bran, rapeseed dregs and fishbone dust is
9:7:4:3；Described compound fixed nitrogen bacterium solution is prepared in accordance with the following steps: by bacillus megaterium, arthrobacterium, the brown fixed nitrogen of circle
Bacterium, bacillus polymyxa cultivate respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 5:4:3:3,
Obtain；
Step 4) prepares weeding biologic product: wheat stalk is ground into wheat stalk powder, adds step 2 to) solution in, stir
Mix uniformly, stands 12 hours, then interpolation dregs of beans, vinasse, oyster shell whiting, potassium dihydrogen phosphate and glucose successively, 500 revs/min
Clock stirs 3 minutes, then the programming rate with 5 DEG C/min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, press
According to 10%(v/v) inoculum concentration inoculation compound herbicide bacterium solution, control at pH6.8-7.2, temperature be 28-30 DEG C condition fermentation 36
Hour, obtain weeding biologic product；Wherein, wheat stalk powder, step 2) solution, dregs of beans, vinasse, oyster shell whiting, biphosphate
The mass ratio of potassium and glucose is 12:90:12:6:6:4:2；
Described compound herbicide bacterium solution is prepared in accordance with the following steps: cultivate trichoderma aureoviride, candidiasis to concentration respectively
It is 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 2:1, to obtain final product；
Step 5) prepares soil conditioner: stir humic acid, peat soil and attapulgite according to the mass ratio mixing of 2:1:1
Mix uniformly, then grind, cross 200 mesh sieves and i.e. obtain soil conditioner；
Step 6) mixes and is dried: by diazotroph preparation, weeding biologic product, soil conditioner according to the mass ratio of 12:9:3
Mixing, stirs, and then freeze-drying is prepared as dry powder, to obtain final product.
Preferably, described bacillus megaterium is CGMCC No.3770, and described arthrobacterium is CGMCC No.7779, described
Azotobacter chroococcum is ATCC No.4412, and bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC No.22023.
Embodiment 3
The field test effect of the biological organic fertilizer of the embodiment of the present invention 1 preparation: as a example by wheat.
Control group: Common compound fertilizer (N-P-K content is 15: 15: 15)；
Test group: the biological organic fertilizer of Common compound fertilizer (N-P-K content is 15: 15: 15)+embodiment 1 preparation.
Experimental technique: select experimental plot Winter Wheat Planted, the equal 10 mu of ground of experimental plot area that two groups of fertilizer is processed respectively.
Control group: Common compound fertilizer usage amount is 40kg, test group: Common compound fertilizer usage amount is that 30kg+10kg implements
The Chemical Mixed Fertilizer of example 1 preparation；Other planting conditions are identical；Gather in the crops wheat simultaneously, measure wheat per mu yield and rate of growth；With
When detection wheat grains per spike, mass of 1000 kernel.Experimental result: with reference to table 1.
Table 1
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 27.3 | 46.7 | 369 |
| Test group | 31.8 | 48.3 | 397 |
Same treatment conditions, Second Year multiple cropping wheat, experimental result: with reference to table 2.
Table 2
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 26.7 | 46.2 | 356 |
| Test group | 32.2 | 48.5 | 411 |
Conclusion: compare discovery by table 1-2, test group wheat grains per spike, mass of 1000 kernel and per mu yield are apparently higher than control group, tool
Standby statistical significance；And the effect of high-yield of Second Year is more preferably, illustrate that biological organic fertilizer effect is more longlasting, flora and soil conditioning
Agent serves fixed nitrogen in soil and protects the effects such as potassium, improves soil quality, it is achieved that sustainable development.
Herbicidal effect: test group is compared with control group, after applying fertilizer 1 month: lady's-grass, lamb's-quarters, purslane, barnyard grass, iron amaranth grass etc.
The quantity of weeds significantly reduces, and wherein, lady's-grass, purslane all decrease 53%, and barnyard grass decreases 65%；Herbicidal effect is good, can generation
Replace or reduce the use of herbicide, and environmentally safe.
Present disclosure is not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention
And the conversion of any equivalence that technical solution of the present invention is taked, the claim being the present invention is covered.
Claims (4)
1. utilizing biological organic fertilizer prepared by glutamic acid fermentation discarded object, it is prepared in accordance with the following steps: step 1) paddy ammonia
Acid fermentation discarded object filters, step 2) prepare nitrogen source, step 3) prepares diazotroph preparation, and step 4) prepares the biological system of weeding
Agent, step 5) prepares soil conditioner, step 6) mixing and drying.
2. biological organic fertilizer according to claim 1, it is characterised in that described biological organic fertilizer is prepared in accordance with the following steps
And obtain:
Step 1) glutamic acid fermentation discarded object filters: the glutamic acid fermentation discarded object producing fermentable glutamic acid filters,
Collect mycoprotein and waste water；
Step 2) prepare nitrogen source: drying mycoprotein, pulverizer is ground into powder, is subsequently placed in retort, adds
The hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
Should use in ammoniacal liquor after terminating and remaining hydrochloric acid, the pH of control solution is 6.8-7.2；
Step 3) prepares diazotroph preparation: pulverizes wheat stalk and obtains wheat stalk powder, then with wheat bran, rapeseed dregs and
Fishbone dust mixing and stirring obtains compound, adds the waste water of the step 1) accounting for compound twice quality, soaks 12 hours, then
Boil 15min, be cooled to room temperature, according to 10%(v/v) the compound fixed nitrogen bacterium solution of inoculum concentration inoculation, at the bar that temperature is 28-30 DEG C
Part ferments 36 hours, obtains diazotroph preparation；Wherein, the mass ratio of wheat stalk powder, wheat bran, rapeseed dregs and fishbone dust is
5-9:4-7:3-4:2-3；
Step 4) prepares weeding biologic product: wheat stalk is ground into wheat stalk powder, adds step 2 to) solution in, stir
Mix uniformly, stands 12 hours, then interpolation dregs of beans, vinasse, oyster shell whiting, potassium dihydrogen phosphate and glucose successively, 500 revs/min
Clock stirs 3 minutes, then the programming rate with 5 DEG C/min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, press
According to 10%(v/v) inoculum concentration inoculation compound herbicide bacterium solution, control at pH6.8-7.2, temperature be 28-30 DEG C condition fermentation 36
Hour, obtain weeding biologic product；Wherein, wheat stalk powder, step 2) solution, dregs of beans, vinasse, oyster shell whiting, biphosphate
The mass ratio of potassium and glucose is 8-12:70-90:10-12:5-6:5-6:3-4:1-2；
Step 5) prepares soil conditioner: stir humic acid, peat soil and attapulgite according to the mass ratio mixing of 2:1:1
Mix uniformly, then grind, cross 200 mesh sieves and i.e. obtain soil conditioner；
Step 6) mixes and is dried: by diazotroph preparation, weeding biologic product, soil conditioner according to 8-12:6-9:2-3's
Mass ratio mixes, and stirs, and then freeze-drying is prepared as dry powder, to obtain final product.
3. biological organic fertilizer according to claim 2, it is characterised in that described compound fixed nitrogen bacterium solution is made in accordance with the following steps
Standby and obtain: bacillus megaterium, arthrobacterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108Individual/
The bacterium solution of ml, then according to the volume ratio mixing of 5:4:3:3, to obtain final product.
4. biological organic fertilizer according to claim 2, it is characterised in that described compound herbicide bacterium solution is made in accordance with the following steps
Standby and obtain: trichoderma aureoviride, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the body of 2:1
The mixing of long-pending ratio, to obtain final product.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106146088A (en) * | 2016-06-30 | 2016-11-23 | 内蒙古阜丰生物科技有限公司 | Utilize the method that amino acids production waste liquid prepares fertilizer |
| CN106187423A (en) * | 2016-06-30 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation garbage |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
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| CN104261947A (en) * | 2014-10-07 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Fertilizer prepared by utilizing threonine fermented wastes |
| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104261947A (en) * | 2014-10-07 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Fertilizer prepared by utilizing threonine fermented wastes |
| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106146088A (en) * | 2016-06-30 | 2016-11-23 | 内蒙古阜丰生物科技有限公司 | Utilize the method that amino acids production waste liquid prepares fertilizer |
| CN106187423A (en) * | 2016-06-30 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation garbage |
| CN106146088B (en) * | 2016-06-30 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | The method for preparing fertilizer using amino acids production waste liquid |
| CN106187423B (en) * | 2016-06-30 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation waste |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
| CN107382622B (en) * | 2017-08-17 | 2021-08-10 | 山东天鹿生物科技有限公司 | Microbial agent containing bacillus polymyxa and application thereof |
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| CN106083490B (en) | 2019-06-14 |
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