CN106116773B - The technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation - Google Patents
The technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation Download PDFInfo
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- CN106116773B CN106116773B CN201610508055.2A CN201610508055A CN106116773B CN 106116773 B CN106116773 B CN 106116773B CN 201610508055 A CN201610508055 A CN 201610508055A CN 106116773 B CN106116773 B CN 106116773B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention belongs to technical field of fertilizers, disclose the technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation, it includes the following steps: that step 1) collects discarded thallus, step 2 hydrolyzes discarded thallus, step 3) prepares fertilizer components A, step 4) prepares fertilizer components B, and step 5) prepares Liquid organic fertilizer.Discarded thallus is utilized in present invention process, realizes waste utilization, and the Liquid organic fertilizer fertilizer efficiency of preparation is lasting.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to discard thallus preparation Liquid organic fertilizer using amino acid fermentation
Technique.
Background technique
Currently, biofermentation enterprise generates a large amount of waste during fermenting and producing various amino acid, due to fermentation
There is COD, SO in amino acid waste4 2-And NH3The features such as N content is high and acid strong, in addition, also containing a large amount of in waste
Mycoprotein, reduced sugar, ammonia nitrogen, if any discharge not only causes serious environmental pollution, but also waste precious resources.
If waste liquid handled, investment is huge, does not also meet recycling economy development requirement.Fermenting and producing amino acid waste is comprehensive
Conjunction uses, and for biofermentation amino acid Sustainable Development of Enterprises, has important strategic importance.Biofermentation is produced
The problem of amino acid waste liquid is processed into organic-inorganic complex fertilizer, that is, the processing for solving waste water is difficult, pollutes environment, and to enterprise
Industry brings significant economic benefit.Applicant early period patented technology " it is a kind of using biofermentation amino acid wastewater prepare it is organic-
Waste liquid is applied in fertilizer preparation by the technique of inorganic composite mixed fertilizer ", solves the problems, such as liquid waste processing, and be prepared for fertilizer, but
It is that the technology still has certain defect, for example, mycoprotein can not be efficiently used, using concentration and evaporation step waste of energy
Deng.
It is Main Stage that the development of China's fertilizer and application history, which are from farmyard manure to inorganic fertilizer is used, because of farmyard manure dirt
Dye source is more, and freight volume is big, and operationally time-consuming and laborious and effect is not particularly evident；And inorganic fertilizer composition is single, utilization rate
It is lower, and often will cause soil hardening and with soil erosion polluted source, influence ecological environment.Currently, market is adopted more
With the inorganic composite fertilizer etc. of Nitrogen-and Phosphorus-containing potassium, the fertilizer efficiency of the compound fertilizer is comprehensive, not lasting but there is also fertilizer efficiency, is easy loss etc. and lacks
It falls into；Importantly, the price of compound fertilizer is higher at present, biggish burden is brought to peasant, how to reduce fertilizer cost, improved
Farmers' income is the agricultural modernization technical issues that need to address.
Summary of the invention
In order to overcome the technological deficiency of the prior art, the object of the present invention is to provide discard thallus system using amino acid fermentation
The technique of standby Liquid organic fertilizer, the discarded thallus of the technology utilization, realizes waste utilization, the Liquid organic fertilizer fertilizer efficiency of preparation is held
Long, and it is environment friendly and pollution-free.
The present invention completes by following technical solution:
The technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation comprising following steps:
Step 1) collects discarded thallus: amino acid zymotic fluid is made using microbial fermentation, filtering fermentating liquid is collected discarded
Filtered solution is used to extract amino acid by thallus and filtered solution, and the waste water that extraction process generates is spare；
Step 2 hydrolyzes discarded thallus: discarded thallus naturally dry being subsequently placed in reactor tank, after being added and drying
Discarded thallus phase homogenous quantities 5mol/L hydrochloric acid, at a temperature of 60 DEG C stirring hydrolysis 24 hours, mixing speed be 300 turns/
Min, in ammonium hydroxide and remaining hydrochloric acid after reaction terminating, the pH for controlling solution is 6.8-7.2；
Step 3) prepares fertilizer components A: broomcorn straw is ground into broomcorn straw powder, is added in the solution of step 2,
It stirs evenly, stands 12 hours, then successively add dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose, 500 turns/
Minutes stirring 3 minutes, then 100 DEG C are heated to the heating rate of 5 DEG C/min, then keep the temperature 30min, cooled to room temperature,
According to 10%(v/v) inoculum concentration inoculating compound bacterium agent A, in pH6.8-7.2, the condition fermentation 36 that temperature is 28-30 DEG C is small for control
When, obtain fertilizer components A；Wherein, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and
The mass ratio of glucose is 25-40:120-180:15-20:10-12:5-7:5-7:2-3；
Step 4) prepare fertilizer components B: broomcorn straw is crushed to obtain broomcorn straw powder, then with wheat bran, manioc waste with
And fishbone dust is mixed evenly to obtain mixture, and the waste water for accounting for the step 1) of twice of mass of mixture is added, and impregnates 12 hours,
Boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind composite bacteria agent B, be 28-30 DEG C of condition in temperature
Fermentation 36 hours, obtains fertilizer components B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 3-7:
2-5:2-5:1-2；
Step 5) prepares Liquid organic fertilizer: fertilizer components A and fertilizer components B being mixed according to the mass ratio of 2-3:3-5, is stirred
Mix uniformly, -4 DEG C of preservations to get.
Preferably, composite bacteria agent A's the preparation method comprises the following steps: by trichoderma aureoviride, pseudomonas aeruginosa, candidiasis point
It Pei Yang be not 1 × 10 to concentration7The bacterium solution of a/ml, then according to 2:2:1 volume ratio mixing to get.
Preferably, the composite bacteria agent B's the preparation method comprises the following steps: by arthrobacterium, bacillus megaterium, bacillus subtilis,
It is 1 × 10 that azotobacter chroococcum, which is cultivated respectively to concentration,8The bacterium solution of a/ml, then according to 3:3:2:2 volume ratio mixing to get.
Preferably, the trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is that CGMCC No.5721(is visible
CN103361284A)；The candidiasis is ATCC No.22023.
Preferably, the arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, described
Bacillus subtilis is CGMCC No.0954, and the azotobacter chroococcum is ATCC No.4412.
Above-mentioned bacterial strains can be obtained from collection or the purchase of other commercial channel.
The beneficial effect that the present invention obtains includes the following aspects:
Direct hydrolysis of the present invention discards thallus as fermentation raw material, provides ammonium chloride and amino acid nitrogen source abundant,
It can not only be used for microbial fermentation nutriment, also can be used as Fertilizer application；
The present invention by fermentation wastes apply all to fertilizer preparation in, avoid to waste be evaporated concentration and
Subtract dirty processing, reduce energy waste, improves utilization rate, and be prepared for fertilizer, kill two birds with one stone；
Bacterial strain compatibility of the present invention is reasonable, using different microbial inoculum different fermentations processing modes, so that different microbial inoculums are in best
Fermentation condition, avoid part bacterial strain cannot waste water prepare culture solution in survive；
The present invention passes through the agricultural waste material of effective use naturally occurring, in conjunction with microbial technique, so that fertilizer cost
Cheap, fertilizer efficiency is more lasting；
By screening and reasonable compatibility, so that fertilizer has fixed nitrogen water conservation, increasing crop yield increases income microbial inoculum of the present invention, also has
Standby certain weeding function, it is environment friendly and pollution-free.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
The technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation comprising following steps:
Step 1) collects discarded thallus: amino acid zymotic fluid is made using microbial fermentation, filtering fermentating liquid is collected discarded
Filtered solution is used to extract amino acid by thallus and filtered solution, and the waste water that extraction process generates is spare；
Step 2 hydrolyzes discarded thallus: discarded thallus naturally dry being subsequently placed in reactor tank, after being added and drying
Discarded thallus phase homogenous quantities 5mol/L hydrochloric acid, at a temperature of 60 DEG C stirring hydrolysis 24 hours, mixing speed be 300 turns/
Min, in ammonium hydroxide and remaining hydrochloric acid after reaction terminating, the pH for controlling solution is 6.8-7.2；
Step 3) prepares fertilizer components A: broomcorn straw is ground into broomcorn straw powder, is added in the solution of step 2,
It stirs evenly, stands 12 hours, then successively add dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose, 500 turns/
Minutes stirring 3 minutes, then 100 DEG C are heated to the heating rate of 5 DEG C/min, then keep the temperature 30min, cooled to room temperature,
According to 10%(v/v) inoculum concentration inoculating compound bacterium agent A, in pH6.8-7.2, the condition fermentation 36 that temperature is 28-30 DEG C is small for control
When, obtain fertilizer components A；Wherein, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and
The mass ratio of glucose is 25:120:15:10:5:5:2；
Step 4) prepare fertilizer components B: broomcorn straw is crushed to obtain broomcorn straw powder, then with wheat bran, manioc waste with
And fishbone dust is mixed evenly to obtain mixture, and the waste water for accounting for the step 1) of twice of mass of mixture is added, and impregnates 12 hours,
Boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind composite bacteria agent B, be 28-30 DEG C of condition in temperature
Fermentation 36 hours, obtains fertilizer components B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 3:2:
2:1；
Step 5) prepares Liquid organic fertilizer: fertilizer components A and fertilizer components B being mixed according to the mass ratio of 2:3, stirring is equal
It is even, -4 DEG C of preservations to get.
Wherein, the composite bacteria agent A's the preparation method comprises the following steps: by trichoderma aureoviride, pseudomonas aeruginosa, candidiasis distinguish
Culture to concentration is 1 × 107The bacterium solution of a/ml, then according to 2:2:1 volume ratio mixing to get；The composite bacteria agent B's
The preparation method comprises the following steps: by arthrobacterium, bacillus megaterium, bacillus subtilis, azotobacter chroococcum cultivate respectively to concentration be 1 ×
108The bacterium solution of a/ml, then according to 3:3:2:2 volume ratio mixing to get.
The trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；The Candida
Bacterium is ATCC No.22023.
The arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, the withered grass bud
Spore bacillus is CGMCC No.0954, and the azotobacter chroococcum is ATCC No.4412.
Embodiment 2
The technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation comprising following steps:
Step 1) collects discarded thallus: amino acid zymotic fluid is made using microbial fermentation, filtering fermentating liquid is collected discarded
Filtered solution is used to extract amino acid by thallus and filtered solution, and the waste water that extraction process generates is spare；
Step 2 hydrolyzes discarded thallus: discarded thallus naturally dry being subsequently placed in reactor tank, after being added and drying
Discarded thallus phase homogenous quantities 5mol/L hydrochloric acid, at a temperature of 60 DEG C stirring hydrolysis 24 hours, mixing speed be 300 turns/
Min, in ammonium hydroxide and remaining hydrochloric acid after reaction terminating, the pH for controlling solution is 6.8-7.2；
Step 3) prepares fertilizer components A: broomcorn straw is ground into broomcorn straw powder, is added in the solution of step 2,
It stirs evenly, stands 12 hours, then successively add dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose, 500 turns/
Minutes stirring 3 minutes, then 100 DEG C are heated to the heating rate of 5 DEG C/min, then keep the temperature 30min, cooled to room temperature,
According to 10%(v/v) inoculum concentration inoculating compound bacterium agent A, in pH6.8-7.2, the condition fermentation 36 that temperature is 28-30 DEG C is small for control
When, obtain fertilizer components A；Wherein, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and
The mass ratio of glucose is 40:180:20:12:7:7:3；
Step 4) prepare fertilizer components B: broomcorn straw is crushed to obtain broomcorn straw powder, then with wheat bran, manioc waste with
And fishbone dust is mixed evenly to obtain mixture, and the waste water for accounting for the step 1) of twice of mass of mixture is added, and impregnates 12 hours,
Boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind composite bacteria agent B, be 28-30 DEG C of condition in temperature
Fermentation 36 hours, obtains fertilizer components B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 7:5:
5:2；
Step 5) prepares Liquid organic fertilizer: fertilizer components A and fertilizer components B being mixed according to the mass ratio of 3:5, stirring is equal
It is even, -4 DEG C of preservations to get.
Composite bacteria agent A's the preparation method comprises the following steps: trichoderma aureoviride, pseudomonas aeruginosa, candidiasis are cultivated respectively
It is 1 × 10 to concentration7The bacterium solution of a/ml, then according to 2:2:1 volume ratio mixing to get.
Composite bacteria agent B's the preparation method comprises the following steps: by arthrobacterium, bacillus megaterium, bacillus subtilis, the brown fixed nitrogen of circle
It is 1 × 10 that bacterium, which is cultivated respectively to concentration,8The bacterium solution of a/ml, then according to 3:3:2:2 volume ratio mixing to get.
The trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；The Candida
Bacterium is ATCC No.22023.
The arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, the withered grass bud
Spore bacillus is CGMCC No.0954, and the azotobacter chroococcum is ATCC No.4412.
Embodiment 3
The field test effect of organic fertilizer prepared by the embodiment of the present invention 1: by taking wheat as an example.
Control group: Common compound fertilizer (N-P-K content 15: 14: 12)；
Test group: the organic fertilizer of Common compound fertilizer (N-P-K content 15: 14: 12)+embodiment 1 preparation.
Experimental method: experimental plot Winter Wheat Planted, the equal 10 mu of ground of the experimental plot area that two groups of fertilizer are handled respectively are selected.
Control group: Common compound fertilizer usage amount is 35kg, and test group: Common compound fertilizer usage amount is 25kg+10kg implementation
Organic fertilizer prepared by example 1；Other planting conditions are identical；Wheat is harvested simultaneously, measures wheat per mu yield and rate of growth；Together
When detect wheat grains per spike, mass of 1000 kernel.Experimental result: referring to table 1.
Table 1
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 27.2 | 45.8 | 355 |
| Test group | 28.9 | 47.3 | 376 |
Same treatment conditions, second year multiple cropping wheat, experimental result: referring to table 2.
Table 2
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 26.5 | 45.1 | 350 |
| Test group | 29.6 | 47.7 | 383 |
Conclusion: by table 1, it was found that, test group wheat grains per spike, mass of 1000 kernel and per mu yield are apparently higher than control group,
Has statistical significance；And the effect of high-yield of second year is more preferably, illustrates that organic fertilizer better effect is lasting, flora rises in soil
The effect of fixed nitrogen water conservation getting fat is arrived.
Herbicidal effect: test group compared with the control group, fertilising 1 month after: the weeds such as purslane, barnyard grass, herba digitariae, iron amaranth grass
Quantity significantly reduce, wherein purslane, herba digitariae reduce 50% or more, and barnyard grass reduces 60% or more；Herbicidal effect is good,
It can replace or reduce the use of herbicide.
The contents of the present invention are not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention
And to any equivalent transformation that technical solution of the present invention is taken, all are covered by the claims of the invention.
Claims (2)
1. the technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation comprising following steps:
Step 1) collects discarded thallus: amino acid zymotic fluid is made using microbial fermentation, filtering fermentating liquid collects discarded thallus
And filtered solution, filtered solution is used to extract amino acid, the waste water that extraction process generates is spare；
Step 2 hydrolyzes discarded thallus: discarded thallus naturally dry is subsequently placed in reactor tank, it is useless after being added and drying
The hydrochloric acid for abandoning the 5mol/L of thallus phase homogenous quantities, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min,
With, with remaining hydrochloric acid, the pH for controlling solution is 6.8-7.2 in ammonium hydroxide after reaction terminating；
Step 3) prepares fertilizer components A: broomcorn straw being ground into broomcorn straw powder, is added in the solution of step 2, is stirred
Uniformly, 12 hours are stood, then successively adds dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose, 500 revs/min
Stirring 3 minutes, then is heated to 100 DEG C with the heating rate of 5 DEG C/min, then keeps the temperature 30min, cooled to room temperature, according to
10% inoculum concentration inoculating compound bacterium agent A, control are fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtain fertilizer
Expect component A；Wherein, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose
Mass ratio is 25-40:120-180:15-20:10-12:5-7:5-7:2-3；
Step 4) prepare fertilizer components B: broomcorn straw is crushed to obtain broomcorn straw powder, then with wheat bran, manioc waste and fish
Bone meal is mixed evenly to obtain mixture, and the waste water for accounting for the step 1) of twice of mass of mixture is added, and impregnates 12 hours, then boil
15min is boiled, is cooled to room temperature, according to 10% inoculum concentration kind composite bacteria agent B, the condition fermentation 36 for being 28-30 DEG C in temperature is small
When, obtain fertilizer components B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 3-7:2-5:2-5:
1-2；
Step 5) prepares Liquid organic fertilizer: fertilizer components A and fertilizer components B being mixed according to the mass ratio of 2-3:3-5, stirring is equal
It is even, -4 DEG C of preservations to get；
Composite bacteria agent A's the preparation method comprises the following steps: being cultivated trichoderma aureoviride, pseudomonas aeruginosa, candidiasis respectively to dense
Degree is 1 × 107The bacterium solution of a/ml, then according to 2:2:1 volume ratio mixing to get；
Composite bacteria agent B's the preparation method comprises the following steps: by arthrobacterium, bacillus megaterium, bacillus subtilis, azotobacter chroococcum point
It Pei Yang be not 1 × 10 to concentration8The bacterium solution of a/ml, then according to 3:3:2:2 volume ratio mixing to get.
2. technique according to claim 1, which is characterized in that the trichoderma aureoviride is ACCC No.32248；Verdigris is false single
Born of the same parents bacterium is CGMCC No.5721；The candidiasis is ATCC No.22023；The arthrobacterium is CGMCC No.7779,
The bacillus megaterium is CGMCC No.3770, and the bacillus subtilis is CGMCC No.0954, the brown fixed nitrogen of circle
Bacterium is ATCC No.4412.
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| CN106613840A (en) * | 2016-12-07 | 2017-05-10 | 广西南宁科茂企业信息咨询有限公司 | Vegetable soilless culture nutrient solution |
| CN109970495A (en) * | 2017-12-28 | 2019-07-05 | 许传高 | A kind of technique preparing organic fertilizer using amino acid fermentation tail washings |
| CN109354521A (en) * | 2018-09-26 | 2019-02-19 | 广东省农业科学院农业资源与环境研究所 | A kind of Amino acid liquid fertilizer mother liquor and preparation method thereof |
| CN110759754B (en) * | 2019-11-05 | 2022-05-31 | 山东润德生物科技有限公司 | Harmless treatment and resource utilization method of glucosamine fermentation bacterium residues |
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| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
| CN104261947B (en) * | 2014-10-07 | 2016-06-29 | 内蒙古阜丰生物科技有限公司 | A kind of fertilizer utilizing Threonine Fermentation garbage to prepare |
| CN106045701A (en) * | 2016-06-07 | 2016-10-26 | 内蒙古阜丰生物科技有限公司 | Compound fertilizer prepared from waste through fermentation with threonine |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104261947B (en) * | 2014-10-07 | 2016-06-29 | 内蒙古阜丰生物科技有限公司 | A kind of fertilizer utilizing Threonine Fermentation garbage to prepare |
| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
| CN106045701A (en) * | 2016-06-07 | 2016-10-26 | 内蒙古阜丰生物科技有限公司 | Compound fertilizer prepared from waste through fermentation with threonine |
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