CN104529613A - Organic biological complex liquid fertilizer and saline-alkali land improvement application method - Google Patents
Organic biological complex liquid fertilizer and saline-alkali land improvement application method Download PDFInfo
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- CN104529613A CN104529613A CN201410717310.5A CN201410717310A CN104529613A CN 104529613 A CN104529613 A CN 104529613A CN 201410717310 A CN201410717310 A CN 201410717310A CN 104529613 A CN104529613 A CN 104529613A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention relates to a preparation method of an organic biological complex liquid fertilizer, and a saline-alkali land improvement application method. The invention belongs to the technical fields of green liquid organic fertilizers and saline-alkali land soil improvement. According to the invention, on a weight basis, 1-50 parts of an amino acid liquid fertilizer and 1-50 parts of a lactobacillus liquid fertilizer are well mixed by stirring under a normal temperature, wherein an amino acid total amount in the amino acid liquid fertilizer is 200-220g/L, and viable cell content in the lactobacillus liquid fertilizer is 1 billion/mL. According to the invention, the two organic fertilizers are effectively combined, such that a synergistic effect greater than the sum of the effects of single fertilizers can be provided for the nutrients of plants.
Description
Technical field
The present invention relates to the composite liquid method for preparing fertilizer of a kind of organic-biological and alkaline land soil improvement application process, belong to green liquid fertilizer and alkaline land soil improving technology field.
Background technology
According to China's development low-carbon (LC), circulation, Organic farming, rescue, improve, repair and plough; Guarantee national food security.The agricultural development planning ensured food safety, in line with improving quality of agricultural product, reducing the object of production cost, research farm crop green bio fertilizer becomes a kind of inexorable trend.Biological organic liquid fertilizer is also the one of biological organic fertilizer.
Biological organic fertilizer is the green animal and plant body extract being used as fertilizer.Biological organic fertilizer is a kind of nutrient green bio source of manure completely.Biological organic fertilizer is not only the effective ways adding the source of manure, and also have great role to improvement soil, specifically, biological organic fertilizer has following advantage:
(1) abundant nutrient can be provided for soil.Various animal and plant body, containing abundant nutrient, once isolate micromolecule polypeptide organism under biochemical action, can increase the organic matter in soil and nitrogen, phosphorus, potassium, calcium, magnesium and various trace element in large quantities.
(2) amino acid and milk-acid bacteria can make difficultly soluble nutrient in soil transform, and are beneficial to absorbing of crop.The organic acid that biological organic liquid fertilizer decomposes generation can make the phosphorus of insoluble in soil, potassium is converted into validity phosphorus, the potassium that crop can utilize.
(3) physical and chemical character of soil can be improved.After biological organic fertilizer is manured into soil, under the effect of microorganism, constantly decompose, except discharging mass efficient nutrient, also form soil ulmin, soil ulmin is combined with calcium and soil cementation can be made to become crumb structure, has the loosing soil of crumb structure, breathes freely, preserve moisture and fertility power is strong, regulates the performance of water, fertilizer, gas, heat; Particularly amino acid and milk-acid bacteria can regulate the pH value trend neutral soil of alkaline land soil; Very good using value is had to the improvement of alkaline land soil; The salinification being conducive to ploughing the northern area of China is repaired with this raw saliferous improvement.
(4) activity of soil microorganisms is promoted.After biological organic fertilizer is manured into soil, adds fresh organic energy substance, microorganism is bred rapidly, movable enhancing, promotes the formation of soil ulmin, the effectuation of nutrient, accelerates soil-ripening.
Current China is studied, as number of patent application: 200980158552.2 disclose a kind of method using poultry blood Preparation of amino acid liquid fertilizer and the Amino acid liquid fertilizer prepared thus biological organic liquid fertilizer is existing.1) collect poultry blood and poultry blood is ground; 2) proteolytic enzyme extracted in soybean is added in the blood of grinding, the blood adding proteolytic enzyme is put into the reactor keeping 50-70 DEG C, carry out reacting about 8-15 hour, the termination reaction when the pH value of blood is 7.5-8.0 under airtight condition, make reactant cool about 3-4 hour.3) at about 25 DEG C-80 DEG C in independent mixing tank, urea, potash, Secondary ammonium phosphate, water-based zinc and EDTA are added to the water, thoroughly this mixture of mixing is to prepare additive agent mixture solution.4) adopt blood obtained in 100 order metre filter second steps, the additive agent mixture solution that the 3rd step is obtained mixes with this filtered blood.
Safety non-pollution of the present invention, organo-mineral complexing improves effective utilization of nutritive element, can be widely used in the cash crop such as corn, cotton, rape, peanut, beans and silkworm and mulberry, tea woods, fruit tree, various vegetables.
As number of patent application: 201010503246.2 disclose a kind of processing method of organic liquid fertilizer.
Waste water, the waste residue of producing bean curd generation are dropped into fermentor tank according to weight ratio 1:0.3-0.6, and under the high temperature of 103-105 DEG C, sterilization 60-80 minute, is then cooled to 30-40 DEG C, makes substratum.Cereuisiae fermentum, subtilis are mixed with the ratio of milk-acid bacteria according to 1:1:1.2, makes mixed strains.Get above-mentioned steps 2 according to the ratio of the 1.0-1.8% of substratum weight) mixed strains, mixed strains is evenly sprayed onto in substratum.Cultivate at temperature 35-40 DEG C, until bubble-free produces in culture medium fermentation tank, namely generate organic liquid fertilizer.
But the fertilizer of above-mentioned preparation is strong to saltings specific aim, effectively can not solve the Soil-sickness Problem in agriculture production, existing milk-acid bacteria rehabilitating soil colloid and crumb structure proterties can not improve pH value; Repair and improve soil physics quality standard, amino acid in soil and lactic acid and animal/vegetable protein rehabilitating soil colloid can not be improved simultaneously, inducing plant can not be led to and produce defensin (alkaloids, flavonoid class, protein-based, organic acid and phenolic compound)
Current existing biological organic liquid fertilizer is not developed for alkaline land soil improvement specially, and its specific aim is not strong.
Summary of the invention
For above the deficiencies in the prior art, the invention provides the composite alkaline land soil improvement liquid fertilizer preparation method of a kind of organic-biological and alkaline land soil improvement application process.This biological organic liquid fertilizer achieves " turning waste into wealth ", " green ", has the function of improvement alkaline land soil physicochemical character, low-carbon (LC) safety; And organic needed for plant, micronutrient element can be supplied comprehensively.
Technical scheme of the present invention is: the composite liquid fertilizer of a kind of organic-biological, and according to the weight part of raw material, get the fertile 1-50 part of amino-acid liquid, milk-acid bacteria Liquid Fertilizer 1-50 part is in normal temperature mixing and stirring; The fertile amino acid contained total amount 200-220g/L of described amino-acid liquid; Milk-acid bacteria Liquid Fertilizer viable bacteria content 1,000,000,000/mL.
Further, described amino-acid liquid is fertile for the present invention program, and weight share forms: the feather suspension 1000 ~ 3000 parts getting uniform state; 3942 neutral proteases 5 ~ 30 parts; 1398 neutral proteinase 5 ~ 30 parts; 166 5 ~ 30 parts, actinomycetes proteolytic enzyme; Pancreatin 2 ~ 10 parts; 5 ~ 30 parts, urea; Sodium bisulfite 1-10 part; Manganous chloride tetrahydrate 1 ~ 10.
Described amino-acid liquid fertilizer is prepared by following steps:
A. poultry feather is prepared into the feather suspension of uniform state;
B. by the feather suspension placing response still for preparing, add urea successively, sodium bisulfite, pancreatin, the stirring velocity maintaining 180 turns/min stirs 2h, and be warming up to 45-47 DEG C, add 3942 neutral proteases and 1398 neutral proteinase, add zymin 166 actinomycetes proteolytic enzyme after maintaining the stirring velocity 12h of 180 turns/min and make suspension, use 30% calcium hydroxide adjust ph to 6.8-7.2, and continue enzymic hydrolysis 18h under 1.1 × 105 ~ 1.1 × 110Pa pressure after, add Manganous chloride tetrahydrate, with 12mol/L sulfuric acid adjust ph to 5.0, be heated to 76-80 DEG C of insulation 5h, pressure glass fibre filter bag machine 600 order filters, it is vegetables that the filter residue collected is rich in amino acid and various trace elements and organic matter, the green organic fertilizer of flowers, the filtrate of collecting is exactly enzymic hydrolysis poultry feather Amino acid liquid fertilizer, aseptic subpackaged warehouse-in, prepared by the amino acid contained total amount 200-220g/L of Amino acid liquid fertilizer that completes.
Further, being prepared as of feather suspension:
1) collected by poultry feather, through impurity elimination, cleaning, stop clearly to water, place air seasoning place and accept solar irradiation and dry, after its moisture about 8-10%, then place in baking oven and dry, bake out temperature is set in 47-52 DEG C and obtains dry feather;
2) the dry feather of preparation is placed the sheet being ground into 1-3mm in pulverizer, example preparation mixed solution in mass ratio, powder Yu Mao ︰ distilled water is 1 ︰ 5, mixed solution after composite is placed in colloidal mill the sheet mixed solution grinding to form 0.02-0.03mm, then sheet mixed solution is placed the feather suspension being prepared into uniform state in homogeneous machine.
Described milk-acid bacteria Liquid Fertilizer, raw material consists of according to weight part: edible white sugar 1-10 part, edible brown sugar 1-10 part, analysis for soybean powder 1-10 part, xanthan gum 1-3 part, modified starch 1-3 part, sucrose ester 1-2 part, high temperature ɑ-starch liquefacation enzyme 0.01-0.03 part, trisodium phosphate 0.001-0.003 part, dipotassium hydrogen phosphate 0.01-0.05 part, above-mentioned materials is mixed, add 73-95 part of purifying waste water, lactobacillus bulgaricus 0.01-0.02 part, thermophilus streptococcus 0.01-0.02 part.
Further, described milk-acid bacteria Liquid Fertilizer produces described milk-acid bacteria liquid fertilizer according to the following steps:
1) white sugar 1-10 part will be eaten by weight, edible brown sugar 1-10 part, analysis for soybean powder 1-10 part xanthan gum 1-3 part, modified starch 1-3 part, sucrose ester 1-2 part, high temperature ɑ-starch liquefacation enzyme 0.01-0.03 part, trisodium phosphate 0.001-0.003 part, dipotassium hydrogen phosphate 0.01-0.05 part, mixes above-mentioned materials, add 73-95 part of purifying waste water and place the suspension being prepared into uniform state in homogeneous machine, then carry out after high-temperature sterilization process as substratum.
2) inoculate one of following bacterial classification or both combinations when liquid medium being cooled to 35-40 DEG C, according to weight part, inoculation measures 0.01-0.04 part; Described bacterial classification is lactobacillus bulgaricus 0.01-0.02 part, thermophilus streptococcus 0.01-0.02 part;
3) placed in culture tank by the inoculation mixed solution of step (2), keep 35-38 DEG C to cultivate 48 hours, when pH value reaches 3.3-4.5, woven fiber glass filters, and collects the preparation that filtrate completes milk-acid bacteria liquid fertilizer;
4) by aseptic subpackaged for the milk-acid bacteria liquid fertilizer of preparation, warehouse-in.
Further, step 1) described in pyroprocessing be keep 30-120min sterilization time at 110-130 DEG C of temperature.
An alkaline land improving application process for the composite liquid fertilizer of organic-biological, carries out to execute with water punching when alkaline land soil improvement is used; The best application process of fertilizer efficiency is: land for growing field crops irrigation water is first imported join fertile pond by the composite alkaline land soil of organic-biological of preparation improvement liquid fertilizer according to: the multiple of fertile material ︰ water=1 ︰ 3000 is added to joins fertile pond; Stagnate 2-4h and carrying out land for growing field crops irrigation when liquid manure fully mixes and water temperature promotes 0.5-3 DEG C.
The composite liquid fertilizer of organic-biological uses according to 25-100 kg/acre of (667 ㎡) amount of application.
Feather of the present invention refers to poultry (chicken and duck goose).
The present invention will be further explained below:
1. the Mechanism of fertilizer efficiency of poultry feather selected by:
(1) poultry feather Advances on Effective Compositions:
In recent years, along with improving constantly of detection technique, and country is to the attention of amino acid medicinal study, research and analyse show that poultry feather contains according to Shanghai University Zhu Xian, Yang Lei: more than the 16 kinds necessary amino acid of animals and plants such as Methionin, proline(Pro), methionine(Met), Threonine, tryptophane, Histidine, glycine, Gelucystine, also containing major element, trace element, VITAMIN and UGF (magnifying thunder).Amino acid is as the monomer of protein, and its application prospect is very wide, and feather protein matter is a kind of insoluble Keratin sulfate, has stronger stability, is generally difficult to hydrolysis.
(2) accelerate growth of microorganism:
The each quasi-microorganism of occurring in nature all needs multiple nutrients material in its vital movement process, mainly comprises water, carbon source, nitrogenous source, mineral element, growth hormone etc.And growth hormone has great importance for microbial growth, because they are prothetic group compositions of enzyme classes in microorganism, once lack these materials, enzyme does not just have vigor, and the vital movement of microorganism also just stopped.And the growth hormone needed for multiple-microorganism such as the ion contained in amino acid, yeast, can promote that these microbial growths are bred better.
(3) amino acid whose bacteriostatic action:
Domestic and international agricultural experts apply the Class of Amino Acid such as tryptophane and are sprayed at action effect plant materials having and resists and eliminate farm crop germ; According to multiple medicine and pharmacology bibliographical information, polypeptide (amino acid whose general name) all has good restraining effect to multiple pathogenic microbes such as dysentery bacterium, diphtheria corynebacterium, Bacillus proteus, bacillus typhi murium, green expander roll bacterium, intestinal bacteria, streptococcus aureus, Bacillus subtilus, cured sample bacillus, eight folded bacillus.
2, selected amino acid whose nutrition Mechanism of fertilizer efficiency:
1) be first amino acid whose physiological function: amino acid is coupled together by peptide bond becomes peptide and protein.Amino acid, peptide and protein are all the histiocytic ultimate constituents of organic life entity, play very important effect to life movement.Some amino acid, except being formed except protein, also participates in the metabolic reaction that some are special, shows the life science characteristic that some is important
2) amino acid whose dissociation property defines amino acid distinctive pH value acid base equilibrium function: amino acid at crystal habit or in aqueous, does not exist with free carboxyl or amine-format, but is dissociated into zwitter-ion.In zwitter-ion, amino is with protonated (-NH
3 +) form existence, carboxyl is with dissociated state (-COO
-) exist.Under different PH conditions.Zwitterionic state also changes thereupon.Amino acid also possesses both sexes and to dissociate character and iso-electric point, when amino acid solution is at certain certain pH value, to make on certain specific amino acid molecule be with positive and negative charge equal, become zwitter-ion; Thus the pH value of balance alkaline land soil progressively tends to neutral.And amino acid is not subject to impact and the interference of Na+ and Na-ion in fused soil and pH value, and other nutritive element that amino acid links directly can be absorbed by crop.
3) Amino acid liquid fertilizer is used for farm crop, and tool is significantly improved output, oil recovery enhancement, reduction pesticide residue, the effect such as to preserve the ecological environment.
3, selected milk-acid bacteria belongs to lactic acid Cordycepps, and this bacterioid is morphologically widely different, and existing elongated rod shape, rod-short have again circular; So kind is all gram-positive microorganism, do not form gemma, and be facultative anaerobe, grow under being adapted at the low or oxygen-free environment of oxygen level, milk-acid bacteria existence and the existence demand that goes down to posterity can be completed by a large amount of release organic acid under alkaline land soil; The pH value that the organic acid that milk-acid bacteria carries and generates can improve saltings progressively tends to neutral.Along with scientist is to the further research of milk-acid bacteria improvement, repair saline-land edatope; Believe and necessarily have very large results.
Compared with prior art, advantage of the present invention is:
The present invention mainly realizes the high yield that alkaline land soil improvement (neutral pH) realizes Organic farming plantation on saltings and increases income (upgrading fall this).Under the future do not used and use chemical fertilizer less, the guarantee Organic farming of Maximum Safety can be gathered in the crops and increase income.
Problem on obstacle does in the company solved in agriculture production.Show: increase soil organic matter content and trace element.Particularly increase plant reproductive growth and the organotrophy element that must absorb of nourishing and growing: such as glucose, fructose, oligose, Nucleotide, VITAMIN, amino acid etc.These organotrophies are all be supplied to plant-growth whole process in the humification process by amino acid and milk-acid bacteria.
The effective element possessed by organic substance self suppresses the harmful microorganism in soil, improves soil microorganisms environment, reaches the chemical quality standard of repairing and improving soil.
By increasing amino acid in soil and milk-acid bacteria and animal/vegetable protein rehabilitating soil colloid and crumb structure proterties, improve P in soil H value; Reach and repair and improve soil physics quality standard.
Certain preventive and therapeutic effect is had to soil-borne disease.Because biogenic sterilant is the plant defense element utilizing some antimicrobial substance or the induction generation contained in some plant, kills or effectively suppress growing of some pathogenic bacteria.Antimicrobial compounds in plant materials is the multiple secondary metabolite with anti-microbial activity that plant materials produces, contain alkaloids, flavonoid class, protein-based, many different types such as organic acid and phenolic compound, as capillin (Capillin), Chinese honey locust glycoside (Saponin).
The organic fertilizer utilizing present method to make has that selected bacterial strain is clear and definite, effectively viable bacteria Tiny ecosystem is stable, content is many and bacterial metabolism thing effect is obvious etc. that advantage production technique is simple, comprehensive become wood low, fertilizer is applied widely.
By adopting effective combination of two kinds of fertilizers, can play to the nutritive ingredient of plant effect that 1+1 is greater than 2.
Embodiment
Embodiment 1:
A kind of organic-biological described in the present embodiment composite alkaline land soil improvement liquid fertilizer, be made up of the raw material of following weight part (kg): according to the weight part of raw material, get fertile 50 parts of amino-acid liquid, milk-acid bacteria Liquid Fertilizer 50 parts is in normal temperature (25 DEG C) mixing and stirring; The fertile amino acid contained total amount 200-220g/L of described amino-acid liquid; Milk-acid bacteria Liquid Fertilizer viable bacteria content 1,000,000,000/mL.
Mixed solution is aseptic, packing; Just complete the preparation of a kind of organic-biological composite alkaline land soil improvement fertilizer.
Wherein amino-acid liquid is fertile, is made up of: the feather suspension 3000 parts getting uniform state the raw material of following weight part (kg); 3942 neutral proteases 15 parts; 1398 neutral proteinase 7.5 parts; 166 7.5 parts, actinomycetes proteolytic enzyme; Pancreatin 3 parts; 15 parts, urea; Sodium bisulfite 10 parts; Manganous chloride tetrahydrate 3 parts.
Preparation method comprises the following steps: operate according to parts by weight of raw materials proportioning:
Step 1): after the collection of bird (chicken, duck, goose) feather, impurity elimination, cleaning (clear water); Place air seasoning place and accept solar irradiation and dry to moisture about 8-10%, then place secondary drying in baking oven, bake out temperature is set in 47-52 DEG C.
Step 2) be ground into 1-3mm by dry feather placement pulverizer; Example preparation mixture powder plumage hair ︰ distilled water=1 ︰ 5 in mass ratio, places the mixture after composite in colloidal mill and grinds to form 0.02-0.03mm mixed solution, then mixed solution is placed the feather suspension being prepared into uniform state in homogeneous machine.
Step 3) by enzymatic reaction (enzymic hydrolysis) Preparation of amino acid.Use zymin kind: 3942 neutral proteases (vigor 200000U/g); 1398 neutral proteinase (vigor 30000U/g); 166 actinomycetes proteolytic enzyme (vigor 30000U/g); Pancreatin (in pancreatin, apoenzyme vigor and the vigor of coenzyme are than being 1:60).
Step 4) the feather suspension 3 tons prepared is placed 5m
3reactor in, add urea 15kg, sodium bisulfite 10kg, the stirring velocity that pancreatin 3kg maintains 180 turns/min stirs 2h and is warming up to 45-47 DEG C and adds 3942 neutral protease 15kg and 1398 neutral proteinase 7.5kg, add zymin 166 actinomycetes proteolytic enzyme 7.5kg after maintaining the stirring velocity 12h of 180 turns/min, use 30% calcium hydroxide regulated by suspension and maintain pH6.8-7.2; Maintain 1.1 × 105 ~ 1.1 × 110Pa pressure continuation enzymic hydrolysis 18h and add Manganous chloride tetrahydrate 3kg, with 12mol/L sulphur acid for adjusting pH to 5.0, be heated to 76-80 DEG C of insulation 5h, pressure glass fibre filter bag machine 600 order filters, and the filter residue collected is rich in the green organic fertilizer that amino acid and various trace elements and organic matter are vegetables, flowers.The filtrate of collecting is exactly that enzymic hydrolysis poultry feather amino-acid liquid is fertile; Aseptic subpackagedly to put in storage.Prepared by the fertile amino acid contained total amount 200-220g/L of the amino-acid liquid that completes.
Milk-acid bacteria Liquid Fertilizer, is made up of the raw material of following weight part (kg): will eat white sugar 10 parts by weight, edible 10 parts, brown sugar, analysis for soybean powder 1 part, xanthan gum 1 part, modified starch 1 part, sucrose ester (SE) 2 parts, high temperature ɑ-starch liquefacation enzyme 0.001 part, trisodium phosphate 0.001 part, dipotassium hydrogen phosphate 0.01 part, above-mentioned materials is mixed, add and purify waste water 85, lactobacillus bulgaricus 0.01 part, thermophilus streptococcus 0.01 part
Preparation method comprises the following steps: operate according to parts by weight of raw materials proportioning:
1) white sugar 10 parts will be eaten by weight, edible 10 parts, brown sugar, analysis for soybean powder 1 part, xanthan gum 1 part, modified starch 1 part, sucrose ester (SE) 2 parts, high temperature ɑ-starch liquefacation enzyme 0.001 part, trisodium phosphate 0.001 part, dipotassium hydrogen phosphate 0.01 part, mixed by above-mentioned materials, adding purifies waste water 85 parts places the suspension being prepared into uniform state in homogeneous machine, then carries out after high-temperature sterilization process (described pyroprocessing keeps 30-120min sterilization time at 110-130 DEG C of temperature) as substratum.
2) inoculate following both bacterial classifications when above-mentioned liquid medium being cooled to 35-40 DEG C to combine, inoculum size is 0.02 part (weight part).Lactobacillus bulgaricus 0.01 part, thermophilus streptococcus 0.01 part.
3) placed in cultivation (fermentation) tank by the inoculation mixed solution of step (2), keep 35-38 DEG C to cultivate 48 hours, when pH value reaches 3.3-4.5, woven fiber glass filters, and collects the preparation that filtrate completes milk-acid bacteria liquid fertilizer.
Prepared by milk-acid bacteria Liquid Fertilizer viable bacteria content 1,000,000,000/mL of completing.
4) by aseptic subpackaged for the milk-acid bacteria liquid fertilizer of preparation, warehouse-in.
Embodiment 2:
The present embodiment, be made up of the raw material of following weight part (kg): amino-acid liquid fertile 40kg, milk-acid bacteria Liquid Fertilizer 20kg, according to weight part by fertile for above-mentioned amino-acid liquid 40kg, milk-acid bacteria Liquid Fertilizer 20kg in normal temperature (25 DEG C) mixing and stirring.Mixed solution is aseptic, packing; Just complete the preparation of a kind of organic-biological composite alkaline land soil improvement fertilizer.
A kind of amino-acid liquid described in the present embodiment is fertile, is made up of the raw material of following weight part (kg):
The feather suspension of uniform state 2000 parts; 3942 neutral proteases (vigor 200000U/g) 20 parts; 1398 neutral proteinase (vigor 30000U/g) 30 parts; 12.5 parts, 166 actinomycetes proteolytic enzyme (vigor 30000U/g); Pancreatin 5 parts.12.5 parts, urea, sodium bisulfite 7.5 parts, Manganous chloride tetrahydrate 5.
Preparation method is identical with embodiment 1 according to the operation of parts by weight of raw materials proportioning.
Milk-acid bacteria Liquid Fertilizer described in the present embodiment, is made up of the raw material of following weight part (kg): will eat white sugar 5 parts by weight, edible 5 parts, brown sugar, analysis for soybean powder 2.5 parts, xanthan gum 2 parts, modified starch 22 parts, sucrose ester (SE) 1 part, high temperature ɑ-starch liquefacation enzyme 0.01 part, trisodium phosphate 0.003 part, dipotassium hydrogen phosphate 0.02 part, above-mentioned materials is mixed, add and purify waste water 91, lactobacillus bulgaricus 0.02 part, thermophilus streptococcus 0.02 part.
Preparation method is identical with embodiment 1 according to the operation of parts by weight of raw materials proportioning.
Embodiment 3:
The composite liquid fertilizer of a kind of organic-biological of the present embodiment, is made up of the raw material of following weight part (kg):
The fertile 30kg of amino-acid liquid, milk-acid bacteria Liquid Fertilizer 40kg operates according to parts by weight of raw materials proportioning: in normal temperature (25 DEG C) mixing and stirring.Mixed solution is aseptic, packing; Just complete the preparation of a kind of organic-biological composite alkaline land soil improvement liquid fertilizer.
Amino-acid liquid is fertile, is made up of: the feather suspension of uniform state 1000 parts the raw material of following weight part (kg); 3942 neutral proteases (vigor 200000U/g) 15 parts; 1398 neutral proteinase (vigor 30000U/g) 25 parts; 18 parts, 166 actinomycetes proteolytic enzyme (vigor 30000U/g); Pancreatin 6 parts; 25 parts, urea; Sodium bisulfite 8 parts; Manganous chloride tetrahydrate 9.
Preparation method is identical with embodiment 1 according to the operation of parts by weight of raw materials proportioning.
Milk-acid bacteria Liquid Fertilizer, is made up of the raw material of following weight part (kg): will eat white sugar 5 parts by weight, edible 5 parts, brown sugar, analysis for soybean powder 2.5 parts, xanthan gum 2 parts, modified starch 22 parts, sucrose ester (SE) 1 part, high temperature ɑ-starch liquefacation enzyme 0.01 part, trisodium phosphate 0.003 part, dipotassium hydrogen phosphate 0.02 part, above-mentioned materials is mixed, add and purify waste water 91, lactobacillus bulgaricus 0.02 part, thermophilus streptococcus 0.02 part.
Preparation method is identical with embodiment 1 according to the operation of parts by weight of raw materials proportioning.
Embodiment 4: application process is tested:
One, test objective
Organic-biological composite alkaline land soil improvement liquid fertilizer is used in Muskmelon Growth rule, Resistant expression and yield result at saltings (pH value 7.54) for exploring muskmelon, design and implement this test, for saltings muskmelon green non-pollution and the production that organises provide scientific basis.
Two, test design
Adopt great Qu simultaneous test, namely whole process uses composite alkaline land soil improvement liquid fertilizer (hereinafter referred to as liquid fertilizer) of organic-biological is process, and whole process uses chemical fertilizer for contrast (ck).Fertilization Structure two processes carries out base fertilizer and using of once topdressing with liquid fertilizer and chemical fertilizer respectively.Process rate of fertilizer application is as table one.The base fertilizer of chemical fertilizer process (ck) is Chemical Mixed Fertilizer (N:P:K=22:11:12) with topdressing.The base fertilizer of liquid fertilizer process is liquid fertilizer with topdressing.Great Qu area is 667m
2; If three repetitions.
Process rate of fertilizer application tested by table one
Unit: kg/acre
Three, for examination material:
For examination melon variety: radix curcumae is fragrant, liquid fertilizer is organic-biological composite alkaline land soil improvement liquid fertilizer, and Chemical Mixed Fertilizer is that the general goods that market is bought is fertile.
Four, for examination field basic condition:
2 groups, Shui Zhai village, frontier passes and mountains town, Yanliang District, Xi'an is positioned at for examination field; Soil type is grey soil property hilltop soil.The low earth of quality, soil fertility is low, pH value 7.54; Front stubble is corn, per unit area yield 464 kilograms; Irrigation condition is medium, and it is convenient to exchange.
Five, organic-biological composite alkaline land soil improvement liquid fertilizer and common chemical fertilizer are on the impact of Muskmelon Plants proterties: (see table 2)
Table 2 liquid fertilizer and chemical fertilizer contrast Muskmelon Plants proterties and test
Six, organic-biological composite alkaline land soil improvement liquid fertilizer and common chemical fertilizer are on the impact of melon fruit proterties: (see table 3)
Table 3 liquid fertilizer and chemical fertilizer contrast melon fruit proterties and test
Seven. organic-biological composite alkaline land soil improvement liquid fertilizer and common chemical fertilizer are on the impact of melon fruit quality: (see table 4)
Table 4 liquid fertilizer and chemical fertilizer contrast melon fruit quality and test
Eight, test-results economic characters and Yield compari@(see table 5):
Table 5, different treatment muskmelon economic characters and Yield compari@(3 are repeated mean value)
Nine, test-results resistance comparative analysis (see table six)
Whole muskmelon is observed and the performance of investigation two process resistance breeding time, key resistance list 6.From table six, the process of organic-biological composite alkaline land soil improvement liquid fertilizer is conducive to improving muskmelon comprehensive resistance, and finally directly or indirectly have influence on muskmelon fruiting rate and single melon weight, the Economic Characters of table five also demonstrates this point.
Table 6 resistance compares
Unit: %
Ten, discuss
1, this test shows, in fertility or the medium field such as low, cultivation muskmelon, mu executes organic-biological composite alkaline land soil improvement liquid fertilizer 100 kilograms, can meet the demand of muskmelon to three large nutritive elements completely, realize increasing production.
2 organic-biological composite alkaline land soil improvement liquid fertilizer, nutrient are complete, and especially organic content is high, and speed is slow, and to have both the lasting period long, muskmelon both can not have been caused to overgrow, also can not cause de-fertile early ageing.
3, relative chemical fertilizer, organic-biological composite alkaline land soil improvement liquid fertilizer fertilizer efficiency is more slow, topdresses and should as far as possible early execute, under namely executing before muskmelon summit of growth occurs, would rather be fertile etc. seedling, can not the fertilizer such as seedling, to meet muskmelon normal growth demand.
11, sum up:
Organic-biological composite alkaline land soil improvement liquid fertilizer and the comparative experiments of common chemical fertilizer from muskmelon at tree characteristics (table 1), fruit properties (table 3), fruit quality (table 4), economic characters and output (table 5), resistance (table 6) aspect: Muskmelon Planting is used organic-biological composite alkaline land soil improvement liquid fertilizer and show good Comprehensive Traits.Be in particular in: growth potential is strong, output is high, fruit glossiness is good, fruit shape is attractive in appearance, disease resistance is strong, the thin meat of skin is thick, sugariness is high, aromatising flavour is dense.Illustrate that in fruits and vegetables are produced, use organic-biological composite alkaline land soil improvement liquid fertilizer decreases the pollution of chemical substance to farm environment thus, save investment of production, be conducive to promoting that green, organic farm products is produced, improve alkaline land soil simultaneously and improve farm crop resistance, optimizing quality.Therefore, Devoting Major Efforts To Developing organic-biological composite alkaline land soil improvement liquid fertilizer is the important step of Developing Sustainable Agriculture and circulation, sustainable agriculture.
Claims (9)
1. the composite liquid fertilizer of organic-biological, is characterized in that, according to the weight part of raw material, get the fertile 1-50 part of amino-acid liquid, milk-acid bacteria Liquid Fertilizer 1-50 part is in normal temperature mixing and stirring; The fertile amino acid contained total amount 200-220g/L of described amino-acid liquid; Milk-acid bacteria Liquid Fertilizer viable bacteria content 1,000,000,000/mL.
2. according to the composite liquid fertilizer of organic-biological described in claim 1, it is characterized in that, described amino-acid liquid is fertile, and weight share forms: the feather suspension 1000 ~ 3000 parts getting uniform state; 3942 neutral proteases 5 ~ 30 parts; 1398 neutral proteinase 5 ~ 30 parts; 166 5 ~ 30 parts, actinomycetes proteolytic enzyme; Pancreatin 2 ~ 10 parts; 5 ~ 30 parts, urea; Sodium bisulfite 1-10 part; Manganous chloride tetrahydrate 1 ~ 10.
3., according to the composite liquid fertilizer of organic-biological according to claim 2, it is characterized in that,
Described amino-acid liquid fertilizer is prepared by following steps:
A. poultry feather is prepared into the feather suspension of uniform state;
B. by the feather suspension placing response still for preparing, add urea successively, sodium bisulfite, pancreatin, the stirring velocity maintaining 180 turns/min stirs 2h, and be warming up to 45-47 DEG C, add 3942 neutral proteases and 1398 neutral proteinase, add zymin 166 actinomycetes proteolytic enzyme after maintaining the stirring velocity 12h of 180 turns/min and make suspension, use 30% calcium hydroxide adjust ph to 6.8-7.2, and continue enzymic hydrolysis 18h under 1.1 × 105 ~ 1.1 × 110Pa pressure after, add Manganous chloride tetrahydrate, with 12mol/L sulfuric acid adjust ph to 5.0, be heated to 76-80 DEG C of insulation 5h, pressure glass fibre filter bag machine 600 order filters, it is vegetables that the filter residue collected is rich in amino acid and various trace elements and organic matter, the green organic fertilizer of flowers, the filtrate of collecting is exactly enzymic hydrolysis poultry feather Amino acid liquid fertilizer, aseptic subpackaged warehouse-in, prepared by the amino acid contained total amount 200-220g/L of Amino acid liquid fertilizer that completes.
4. according to the composite liquid fertilizer of organic-biological according to claim 3, it is characterized in that, being prepared as of feather suspension:
1) collected by poultry feather, through impurity elimination, cleaning, stop clearly to water, place air seasoning place and accept solar irradiation and dry, after its moisture about 8-10%, then place in baking oven and dry, bake out temperature is set in 47-52 DEG C and obtains dry feather;
2) the dry feather of preparation is placed the sheet being ground into 1-3mm in pulverizer, example preparation mixed solution in mass ratio, powder Yu Mao ︰ distilled water is 1 ︰ 5, mixed solution after composite is placed in colloidal mill the sheet mixed solution grinding to form 0.02-0.03mm, then sheet mixed solution is placed the feather suspension being prepared into uniform state in homogeneous machine.
5. according to the composite liquid fertilizer of organic-biological described in claim 1, it is characterized in that, described milk-acid bacteria Liquid Fertilizer, raw material consists of according to weight part: edible white sugar 1-10 part, edible brown sugar 1-10 part, analysis for soybean powder 1-10 part, xanthan gum 1-3 part, modified starch 1-3 part, sucrose ester 1-2 part, high temperature ɑ-starch liquefacation enzyme 0.01-0.03 part, trisodium phosphate 0.001-0.003 part, dipotassium hydrogen phosphate 0.01-0.05 part, above-mentioned materials is mixed, add 73-95 part of purifying waste water, lactobacillus bulgaricus 0.01-0.02 part, thermophilus streptococcus 0.01-0.02 part.
6. according to the composite liquid fertilizer of organic-biological described in claim 5, it is characterized in that, described milk-acid bacteria Liquid Fertilizer produces described milk-acid bacteria liquid fertilizer according to the following steps:
1) white sugar 1-10 part will be eaten by weight, edible brown sugar 1-10 part, analysis for soybean powder 1-10 part xanthan gum 1-3 part, modified starch 1-3 part, sucrose ester 1-2 part, high temperature ɑ-starch liquefacation enzyme 0.01-0.03 part, trisodium phosphate 0.001-0.003 part, dipotassium hydrogen phosphate 0.01-0.05 part, mixes above-mentioned materials, add 73-95 part of purifying waste water and place the suspension being prepared into uniform state in homogeneous machine, then carry out after high-temperature sterilization process as substratum.
2) inoculate one of following bacterial classification or both combinations when liquid medium being cooled to 35-40 DEG C, according to weight part, inoculation measures 0.01-0.04 part; Described bacterial classification is lactobacillus bulgaricus 0.01-0.02 part, thermophilus streptococcus 0.01-0.02 part;
3) placed in culture tank by the inoculation mixed solution of step (2), keep 35-38 DEG C to cultivate 48 hours, when pH value reaches 3.3-4.5, woven fiber glass filters, and collects the preparation that filtrate completes milk-acid bacteria liquid fertilizer;
4) by aseptic subpackaged for the milk-acid bacteria liquid fertilizer of preparation, warehouse-in.
7., according to the composite liquid fertilizer of organic-biological according to claim 6, it is characterized in that, step 1) described in pyroprocessing be keep 30-120min sterilization time at 110-130 DEG C of temperature.
8. an alkaline land improving application process for the composite liquid fertilizer of organic-biological, is characterized in that: carry out to execute with water punching when alkaline land soil improvement is used; The best application process of fertilizer efficiency is: land for growing field crops irrigation water is first imported join fertile pond by the composite alkaline land soil of organic-biological of preparation improvement liquid fertilizer according to: the multiple of fertile material ︰ water=1 ︰ 3000 is added to joins fertile pond; Stagnate 2-4h and carrying out land for growing field crops irrigation when liquid manure fully mixes and water temperature promotes 0.5-3 DEG C.
9. according to the alkaline land improving application process of the composite liquid fertilizer of organic-biological according to claim 8, it is characterized in that: the composite liquid fertilizer of organic-biological uses according to 25-100 kg/acre of amount of application.
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