CN108947614A - It is a kind of to utilize liquid fertilizer made of streptococcus acidi lactici fermented solution - Google Patents
It is a kind of to utilize liquid fertilizer made of streptococcus acidi lactici fermented solution Download PDFInfo
- Publication number
- CN108947614A CN108947614A CN201810605153.7A CN201810605153A CN108947614A CN 108947614 A CN108947614 A CN 108947614A CN 201810605153 A CN201810605153 A CN 201810605153A CN 108947614 A CN108947614 A CN 108947614A
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- CN
- China
- Prior art keywords
- fermentation
- liquid fertilizer
- temperature
- acidi lactici
- streptococcus acidi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
Liquid fertilizer made of streptococcus acidi lactici fermented solution is utilized the present invention relates to a kind of, the application is using liquid fertilizer made of streptococcus acidi lactici fermented solution, wherein being rich in organic nitrogen and inorganic salts nitrogen, phosphorus and potassium, while being rich in trace element magnesium and manganese, as growth factor, the growth of plant can be promoted.Liquid fertilizer is made of fermented waste fluid, will to can be used for flowers and plants, crops and trees.Fertilizer efficiency is rapidly and lasting, and production process solves the problems, such as that fermented waste fluid discharge brings environmental hazard and processing cost high without any pollution.
Description
Technical field
The present invention relates to a kind of fermented type liquid fertilizers.
Background technique
Lactobacillus-fermented can all generate a large amount of fermented waste fluid, and being discharged into rivers and lakes will cause eutrophication, lead
Wawter bloom is caused to occur, blue algae bloom.If very big energy consumption will be will cause by discharging again by sewage treatment.The present invention solves
Liquid fertilizer is made in fermentation liquid by double-barreled question, has not only been avoided to be discharged into natural environment and is polluted, but also can reduce sewage
Bring energy consumption is handled, is turned waste into wealth, additionally it is possible to generate certain economic benefit.
Summary of the invention
The technical problem to be solved by the present invention is to provide a kind of to utilize liquid made of fermentation liquid for the above-mentioned prior art
Body fertilizer, very nutritious, not only organic principle rich in, but also contain electrodeless salt component very rich can be to plant
Object provides nitrogen, phosphorus, potassium, magnesium, manganese and growth factor.
The present invention solves the above problems used technical solution are as follows: a kind of to utilize Liquid Fertilizer made of streptococcus acidi lactici fermented solution
Material, which be prepared by following steps
(1) molten by mass parts preparation potato starch 1-10%, carbohydrase 1-5%, soy meal 1-10%, protease 1-5%
In Xie Yushui, 30-180 minutes are kept the temperature at a temperature of 25-85 DEG C, obtains culture solution just liquid;
(2) yeast powder 1-10%, peptone 1-10%, glucose 1-10%, powdered beef 1-10%, phosphoric acid is further added
Potassium dihydrogen 1-10%, diammonium hydrogen citrate 1-10%, magnesium sulfate 1-5%, manganese sulfate 1-5% obtain culture solution, add as needed
Water;
(3) to culture solution high-temperature sterilization: temperature is 115 DEG C -121 DEG C, sterilization time 20-30 minutes, is then cooled to 32
℃-42℃;
(4) lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosus, lactobacillus reuteri, thermophilic is accessed into fermentation system
One of Lactobacillus lactis, Lactococcus lactis are a variety of, milk fermentation 18-36 hours in 25-50 DEG C of temperature range;
(5) the resulting fermentation liquid of step (4) is centrifuged, gained fermentation liquid is heat-treated 30-60 points at a temperature of 55-65 DEG C
Then fermentation liquid is squeezed into temporary storage tank by clock, adjust pH to 6.5- with one or more kinds of in ammonium hydroxide, sodium hydroxide, sodium bicarbonate
7.5;
(6) xanthomonas campestris, geotrichum candidum progress secondary fermentation are then added, it is canned after fermentation 25-50 hours to obtain
To the liquid fertilizer.
Specifically, the adding proportion of step (6) xanthomonas campestris is 1-5%, and the adding proportion of geotrichum candidum is 1-
5%.
Streptococcus acidi lactici fermented solution used herein refers to after lactobacillus-fermented, removes through centrifugation and ferments obtained from thallus
Supernatant.
The pH of fermentation liquid is adjusted to 6.5-7.5, makes to be in neutrality, is in order to avoid salt ionic concentration inside final fermentation liquid
It is excessively high.
The application has been previously added potassium dihydrogen phosphate 1-10%, diammonium hydrogen citrate 1-10% in culture solution, improves
The nutrients such as inorganic salts, nitrogen, phosphorus and potassium in fermentation liquid, improve the whole ion concentration of fermentation liquid.
Liquid fertilizer made from the application is rich in trace element magnesium and manganese, facilitates the improvement to soil, facilitate plant
Growth.
The application carries out secondary fermentation using xanthomonas campestris, geotrichum candidum, and xanthomonas campestris can produce solidifying
Micelle ensure that the viscosity and homogeneity of product, can prevent albumen and other species precipitates;Geotrichum candidum protein content is high,
It is wide to produce temperature, production pH range is wide, can produce high-quality protein, the supplement as soil and fertilizer.Secondary fermentation mainly for
Albumen is generated using the residual sugar of culture medium, increases the nitrogen content of fertilizer.
Compared with the prior art, the advantages of the present invention are as follows: the application is to can be used for spending using made of fermented waste fluid
Grass, crops and trees.Fertilizer efficiency is rapidly and lasting, and production process is without any pollution.It can be avoided fermented waste fluid direct emission band
Come environmental hazard and processing cost.
Specific embodiment
Present invention is further described in detail with reference to embodiments.
Embodiment 1
A kind of to utilize liquid fertilizer made of streptococcus acidi lactici fermented solution, preparation step is as follows
(1) prepare potato starch 5%, carbohydrase 1%, soy meal 2%, protease 2% by mass parts to be dissolved in the water,
180 minutes are kept the temperature at a temperature of 28 DEG C;
(2) yeast powder 1%, peptone 1%, glucose 2%, powdered beef 1%, potassium dihydrogen phosphate 2%, lemon is further added
Lemon acid hydrogen diammonium 1%, magnesium sulfate 1%, manganese sulfate 1% obtain culture solution, and system is diluted half by water supplement;
(3) to culture solution high-temperature sterilization: temperature be 115 DEG C -121 DEG C, sterilization time 30 minutes, be then cooled to 32 DEG C -
In 42 DEG C of temperature ranges;
(4) lactobacillus plantarum, Lactobacillus casei are accessed into fermentation system, milk fermentation 24 is small in 30 DEG C of temperature ranges
When;
(5) the resulting fermentation liquid of step (4) is centrifuged, supernatant is heat-treated 30-60 minutes at a temperature of 55-65 DEG C, so
After squeeze into temporary storage tank using ammonium hydroxide, sodium hydroxide adjust pH to 6.5-7.5;
(6) xanthomonas campestris 3%, the progress secondary fermentation of geotrichum candidum 2% are then added, it is canned after fermentation 48 hours
Obtain liquid fertilizer, which reaches 6.8%, after secondary fermentation N content compared to improve 0.6 before fermentation~
1.2%, phosphorus, potassium content are abundant.
Embodiment 2
A kind of to utilize liquid fertilizer made of streptococcus acidi lactici fermented solution, preparation step is as follows
(1) prepare potato starch 4%, carbohydrase 1%, soy meal 4%, protease 4% by mass parts to be dissolved in the water,
30 minutes are kept the temperature at a temperature of 60 DEG C;
(2) yeast powder 2%, peptone 4%, glucose 2%, powdered beef 4%, potassium dihydrogen phosphate 1%, lemon is further added
Lemon acid hydrogen diammonium 1%, magnesium sulfate 1%, manganese sulfate 1% obtain culture solution, water supplement, and amount of water is the 55% of system;
(3) to culture solution high-temperature sterilization: temperature be 115 DEG C -118 DEG C, sterilization time 30 minutes, be then cooled to 32 DEG C -
Within the scope of 42 DEG C;
(4) Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus are accessed into fermentation system, in 25-50 DEG C of temperature
Milk fermentation 18-36 hours in range;
(5) fermentation liquid is centrifuged, supernatant is heat-treated 50 minutes at a temperature of 55 DEG C, then squeezes into fermentation liquid temporary
Tank adjusts pH to 6.5-7.5 using sodium bicarbonate;
(6) xanthomonas campestris 2%, the progress secondary fermentation of geotrichum candidum 2% are then added, it is canned after fermentation 48 hours
Liquid fertilizer is obtained, the N content of gained liquid fertilizer is 6.6%, and phosphorus, potassium content are abundant.
Embodiment 3
A kind of to utilize liquid fertilizer made of streptococcus acidi lactici fermented solution, preparation step includes
(1) prepare potato starch 6%, carbohydrase 5%, soy meal 4%, protease 1% by mass parts to be dissolved in the water,
60 minutes are kept the temperature at a temperature of 25-85 DEG C;
(2) further be added yeast powder 2%, peptone 8%, glucose 8%, powdered beef 10%, potassium dihydrogen phosphate 4%,
Diammonium hydrogen citrate 1%, magnesium sulfate 1%, manganese sulfate 1% obtain culture solution, the water of 2 times of system of addition;
(3) to culture solution high-temperature sterilization: temperature be 115 DEG C -121 DEG C, sterilization time 30 minutes, be then cooled to 32 DEG C -
Within the scope of 42 DEG C;
(4) lactobacillus plantarum, lactobacillus acidophilus, Lactococcus lactis are accessed into fermentation system, in 50 DEG C of temperature ranges
Milk fermentation 24 hours;
(5) fermentation liquid is centrifuged, supernatant is heat-treated 30 minutes at a temperature of 65 DEG C, temporary storage tank is then squeezed into, using ammonia
Water adjusts pH to 6.5-7.5;
(6) xanthomonas campestris 2%, the progress secondary fermentation of geotrichum candidum 4% are then added, it is canned after fermentation 50 hours
Obtain the liquid fertilizer, the N content 6.8% of liquid fertilizer.
Liquid fertilizer is used for the improvement of mountain soil, can adapt to fruit tree, such as orange, the plantation of orange.
In addition to the implementation, all to use equivalent transformation or equivalent replacement the invention also includes there is an other embodiments
The technical solution that mode is formed should all be fallen within the scope of the hereto appended claims.
Claims (3)
1. a kind of utilize liquid fertilizer made of streptococcus acidi lactici fermented solution, it is characterised in that: the liquid fertilizer is by following steps system
It is standby to form
(1) prepare potato starch 1-10%, carbohydrase 1-5%, soy meal 1-10%, protease 1-5% by mass parts to be dissolved in
In water, 30-180 minutes are kept the temperature at a temperature of 25-85 DEG C;
(2) yeast powder 1-10%, peptone 1-10%, glucose 1-10%, powdered beef 1-10%, biphosphate is further added
Potassium 1-10%, diammonium hydrogen citrate 1-10%, magnesium sulfate 1-5%, manganese sulfate 1-5% obtain culture solution, as needed water supplement;
(3) to culture solution high-temperature sterilization: temperature is 115 DEG C -121 DEG C, sterilization time 20-30 minutes, is then cooled to 32 DEG C -42
℃;
(4) lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosus, lactobacillus reuteri, acidophilus cream are accessed into fermentation system
One of bacillus, Lactococcus lactis are a variety of, milk fermentation 18-36 hours in 25-50 DEG C of temperature range;
(5) the resulting fermentation liquid of step (4) is centrifuged, gained fermentation liquid is heat-treated 30-60 minutes at a temperature of 55-65 DEG C, so
Fermentation liquid is squeezed into temporary storage tank afterwards, adjusts pH to 6.5-7.5;
(6) xanthomonas campestris, geotrichum candidum progress secondary fermentation are then added, it is canned after fermentation 25-50 hours to obtain institute
The liquid fertilizer stated.
2. according to claim 1 utilize liquid fertilizer made of streptococcus acidi lactici fermented solution, it is characterised in that: in step (5)
Using the pH to adjust fermentation liquid one or more kinds of in ammonium hydroxide, sodium hydroxide, sodium bicarbonate.
3. according to claim 1 utilize liquid fertilizer made of streptococcus acidi lactici fermented solution, it is characterised in that: step (6) is wild
The adding proportion of campestris is 1-5%, and the adding proportion of geotrichum candidum is 1-5%.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109593010A (en) * | 2019-01-07 | 2019-04-09 | 天津新平台生物科技有限公司 | A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer |
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JP2001199782A (en) * | 2000-01-12 | 2001-07-24 | Marutomo:Kk | Method for producing fertilizer and compost having crumb structure |
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CN104529613A (en) * | 2014-11-29 | 2015-04-22 | 沈阳金尔露生物科技有限公司 | Organic biological complex liquid fertilizer and saline-alkali land improvement application method |
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CN106748336A (en) * | 2017-03-16 | 2017-05-31 | 新疆农神生物工程有限公司 | Liquid fertilizer containing streptococcus acidi lactici fermented solution |
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2018
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109593010A (en) * | 2019-01-07 | 2019-04-09 | 天津新平台生物科技有限公司 | A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer |
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